CN104857435A - Chinese herbal compound composition with antineoplastic activity as well as preparation method and application thereof - Google Patents
Chinese herbal compound composition with antineoplastic activity as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses Chinese herbal compound composition with antineoplastic activity as well as a preparation method and an application thereof. The Chinese herbal compound composition is prepared from 12-36 parts of heterophylly falsestarwort roots, 10-30 parts of radix ophiopogonis, 20-60 parts of hedyotis diffusa, 20-60 parts of barbed skullcap herb, 15-45 parts of Indian iphigenia bulb and 10-30 parts of rhizoma curcumae. A pharmacology experiment and a clinical experiment indicate that the Chinese herbal compound composition with the antineoplastic activity is scientific and reasonable in matching and has efficacy of resisting cancer, removing toxicity, reducing phlegm, removing stasis and tonifying qi and yin, has a strong killing function on malignant tumor cells and is wide in application range; the content of cAMP (cyclic adenosine monophosphate) in a body can be adjusted, TNF (tumor necrosis factor) generation is accelerated, the immunity of a tumor patient is improved, the lifetime is prolonged, the safety is high, and the toxic and side effects are low. According to the preparation method, the quality is stable, the content of effective extracted ingredients is high, the operatability is high, and the Chinese herbal compound composition is suitable for industrial mass production.
Description
Technical field
The present invention relates to a kind of Chinese medicine, be specifically related to a kind of Traditional Chinese medicine compound composition with anti-tumor activity and its preparation method and application.
Background technology
Tumor has become the number one killer that 21 century threatens human survival, has more than 600 ten thousand people to die from various tumor disease every year on average.China is one of world's cancer country occurred frequently, and presents a rapidly rising trend every year.The medicine for the treatment of tumor clinically at present mainly contains chemical drugs, but because chemotherapeutics toxicity is large, while killing tumor cell also non-selectivity kill the human autoimmune such as leukocyte, lymphocyte cell, cause patient usually due to because of the accompanying infection of autoimmune inferior capabilities, patient uses erious adverse reaction, and the course for the treatment of is long, costly, in Clinical practice, larger limitation is had.
Chinese medicine has unique advantage in treatment tumor, is that the natural class medicine of representative has the features such as toxic and side effects is little, action target spot is various, better tolerance with Chinese medicine, more and more comes into one's own in treatment tumor.But the antitumor drug dosage of report is comparatively large at present, and impurity component is more.
Therefore, research and develop a kind of antitumous effect good, dosage is few, all has good therapeutic effect to kinds of tumors, and the low antineoplastic Chinese traditional medicine of untoward reaction is significant.
Summary of the invention
Goal of the invention: the object of the invention is to solve the deficiencies in the prior art, there is provided a kind of proportioning scientific and reasonable, determined curative effect, Clinical practice safety, the Traditional Chinese medicine compound composition of what untoward reaction was low have anti-tumor activity, another object of the present invention is to provide this preparation method with the Traditional Chinese medicine compound composition of anti-tumor activity and the application in preparation treatment malignancy disease medicine thereof.
Technical scheme: in order to realize above object, the technical scheme that the present invention takes is:
Have a Traditional Chinese medicine compound composition for anti-tumor activity, it is made up of the crude drug of following parts by weight: Radix Pseudostellariae 12 ~ 36 parts, Radix Ophiopogonis 10 ~ 30 parts, Herba Hedyotidis Diffusae 20 ~ 60 parts, Herba Scutellariae Barbatae 20 ~ 60 parts, Pseudobulbus Cremastrae Seu Pleiones 15 ~ 45 parts, Rhizoma Curcumae 10 ~ 30 parts.
Preferably, the above-described Traditional Chinese medicine compound composition with anti-tumor activity, it is made up of the crude drug of following parts by weight: Radix Pseudostellariae 12 ~ 24 parts, Radix Ophiopogonis 10 ~ 20 parts, Herba Hedyotidis Diffusae 20 ~ 40 parts, Herba Scutellariae Barbatae 20 ~ 40 parts, Pseudobulbus Cremastrae Seu Pleiones 15 ~ 30 parts, Rhizoma Curcumae 10 ~ 20 parts.
As being more preferably scheme, the above-described Traditional Chinese medicine compound composition with anti-tumor activity, it is made up of the crude drug of following parts by weight: Radix Pseudostellariae 12 parts, Radix Ophiopogonis 10 parts, Herba Hedyotidis Diffusae 20 parts, Herba Scutellariae Barbatae 20 parts, Pseudobulbus Cremastrae Seu Pleiones 15 parts, Rhizoma Curcumae 10 parts.
The Traditional Chinese medicine compound composition with anti-tumor activity of the present invention, is prepared into the medicine of oral liquid, granule, capsule or Tabules by the extract of each crude drug and pharmaceutically acceptable carrier.
The preparation method with the Traditional Chinese medicine compound composition of anti-tumor activity of the present invention, it comprises the following steps:
(1) get Rhizoma Curcumae by weight, add the water of Rhizoma Curcumae medical material 8 ~ 16 times amount, adopt extraction by steam distillation 1 ~ 3 hour, collect volatile oil;
(2) Radix Pseudostellariae is got by weight, Radix Ophiopogonis, Herba Hedyotidis Diffusae, Herba Scutellariae Barbatae and Pseudobulbus Cremastrae Seu Pleiones, add the Rhizoma Curcumae medicinal residues after step (1) vapor distillation, add the ethanol of total medical material weight 6 ~ 12 times of volumes, backflow or continuous circumfluence extraction 2 ~ 3 times, each 1 ~ 3 hour, merge extractive liquid, 50 ~ 80 degrees Celsius of lower concentrating under reduced pressure, obtain macroporous adsorptive resins on concentrate, first washing with water, is then 20% ~ 70% ethanol elution by volumetric concentration successively; Collecting merging volumetric concentration is the ethanol elution of 55%, concentrates and to obtain extract;
(3) get the volatile oil that step (1) obtains, the extract that step (2) obtains and pharmaceutically acceptable carrier are prepared into the medicine of oral liquid, granule, capsule or Tabules.
When the extract with each raw material of the Traditional Chinese medicine compound composition of anti-tumor activity provided by the invention is made oral liquid, the extract adding distil water of each raw material is dissolved, filter, add syrup, adjust ph, make oral liquid.
When the extract with each raw material of the Traditional Chinese medicine compound composition of anti-tumor activity provided by the invention is made tablet, the extract of each raw material and lactose or corn starch, add magnesium stearate lubricant when needing, mix homogeneously, granulate, then tablet made by tabletting.
The extract of each raw material and carrier lactose or corn starch mix homogeneously when the extract with each raw material of the Traditional Chinese medicine compound composition of anti-tumor activity provided by the invention makes capsule, granulate, then encapsulatedly makes capsule.
When the extract with each raw material of the Traditional Chinese medicine compound composition of anti-tumor activity provided by the invention makes granule, the extract of each raw material and diluent lactose or corn starch mix homogeneously, granulate, dry, make granule.
Preferably, the above-described preparation method with the Traditional Chinese medicine compound composition of anti-tumor activity, the macroporous adsorbent resin described in step (1) is D101 resin.
The Traditional Chinese medicine compound composition with anti-tumor activity of the present invention is preparing the application in antitumor drug.
Preferably, the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention in the anti-pulmonary carcinoma of preparation, gastric cancer, hepatocarcinoma, colon cancer, breast carcinoma, the application in cervical cancer or human tongue carcinoma medicine.
The Chinese medicine composition with anti-lung cancer activity provided by the invention, Radix Pseudostellariae nature and flavor: sweet, micro-hardship; Cold nature., Gui Jing: spleen channel, lung meridian.Effect: mend strengthening spleen and lung; Supplementing QI for promoting the production of body fluid.Cure mainly: weakness of the spleen and stomach; Inappetence; Lassitude; Impairment of both QI and YIN; Dry cough expectorant is few, and the convalescent stage of febrile disease deficiency of vital energy Tianjin wound; Interior-heat is thirsty.
Radix Ophiopogonis, sweet micro-hardship, cold, enters lung meridian, stomach warp, heart channel.Have nourishing YIN and moistening the lung, clear away heart-fire relieving restlessness, reinforcing stomach reg fluid effect, and have treatment dryness of the lung dry cough, spit blood, spitting of blood, consumptive lung disease, lung abscess, asthenia dysphoria with smothery sensation, quenches one's thirst, and calentura Tianjin is hindered, dry mouth and throat, constipation effect.
Herba Hedyotidis Diffusae hardship is sweet, cold.Enter heart channel, Liver Channel, spleen channel.There is heat-clearing and toxic substances removing; Dampness removing effect, has treatment dyspnea and cough due to lung-heat; Laryngopharynx swelling and pain; Intestinal carbuncle; Furuncle and phyma skin infection; Venom; The puckery pain of pyretic stranguria; Edema; Dysentery; Intestinal is scorching; Jaundice due to damp-heat; Cancerous protuberance effect.
Pseudobulbus Cremastrae Seu Pleiones nature and flavor: sweet in the mouth; Micro-pungent; Cold in nature; Return Liver Channel; Stomach warp; Lung meridian.There is heat-clearing and toxic substances removing; Dispersing swelling and dissipating binds effect, has treatment carbuncle malignant boil; Scrofula tuberculosis; Pharyngalgia sore throat effect.
Herba Scutellariae Barbatae nature and flavor: acrid in the mouth; Bitter; Cold in nature.Effect: heat-clearing and toxic substances removing; Dissipating blood stasis stops blooding; Inducing diuresis to remove edema.Cure mainly: pyretic toxicity carbuncle; Have sore throat; Lung abscess; Intestinal carbuncle; Scrofula; Venom; Traumatic injury; Spit blood; Epistaxis; Stranguria with blood; Edema; Ascites and cancer.
Rhizoma Curcumae acrid in the mouth, hardship, temperature.Return liver, spleen channel.There is removing blood stasis circulation of qi promoting, removing food stagnancy pain relieving, expelling wind and removing dampness, reducing swelling and alleviating pain effect.For rheumatalgia, wind syndrome of head pain, chest and hypochondrium, diarrhoea pain; Traumatic injury is controlled in external, and swelling and pain due to blood stasis is treated.
All medicines share effects such as having good anticancer removing toxic substances, dissipating phlegm and removing blood stasis, supplementing QI and nourishing YIN above, have the growth of good inhibition tumor cell and the effect that can promote apoptosis of tumor cells.
Beneficial effect: compared to the prior art the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention has the following advantages:
1, the present invention is according to Chinese medical theory, and adopt the theory of determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs to carry out Chinese prescription screening, the Traditional Chinese medicine compound composition proportioning with anti-tumor activity provided is scientific and reasonable, has the effects such as good anticancer removing toxic substances, dissipating phlegm and removing blood stasis, supplementing QI and nourishing YIN.Experimental result shows, the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention has the growth of good inhibition tumor cell and can promote the effect of apoptosis of tumor cells, and to Several Kinds of Malignancy cell, all there is strong killing action, have wide range of applications.
2, experimental result shows that the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention has the body resistance strengthening and constitution consolidating and immunologic function facilitation, and the generation of cAMP content and promotion tumor necrosis factor (INF) in adjustable body, play Mutiple Targets, the effect of multiple organ treatment tumor, tumor patient misery can be reduced, extend life cycle, improve the effect of life in patients, and show through long-term experiment research, the Traditional Chinese medicine compound composition safety with anti-tumor activity provided by the invention is high, and toxic and side effects is low.
3, the extract with the Traditional Chinese medicine compound composition of anti-tumor activity provided by the invention can facilitate and pharmaceutical carriers is prepared into multiple dosage form, taking convenience clinically; The preparation method with the Traditional Chinese medicine compound composition of anti-tumor activity provided by the invention simultaneously, workable, especially according to each herbal medicine effective ingredient chemical property design extraction and purification process, the extract active constituent content obtained is high, impurity is few, and have absorb fast, drug effect can be played rapidly, the advantages such as dosage is few clinically, and process stabilizing, be applicable to industrialized great production.
Detailed description of the invention:
The present invention is illustrated further below in conjunction with specific embodiment, these embodiments should be understood only be not used in for illustration of the present invention and limit the scope of the invention, after having read the present invention, the amendment of those skilled in the art to the various equivalent form of value of the present invention has all fallen within the application's claims limited range.
Implementation column 1
1. have a Traditional Chinese medicine compound composition for anti-tumor activity, it is made up of the crude drug of following parts by weight: Radix Pseudostellariae 12 grams, Radix Ophiopogonis 10 grams, Herba Hedyotidis Diffusae 20 grams, Herba Scutellariae Barbatae 20 grams, Pseudobulbus Cremastrae Seu Pleiones 15 grams, Rhizoma Curcumae 10 grams.
2. the preparation method with the Traditional Chinese medicine compound composition of anti-tumor activity of the present invention, it comprises the following steps:
(1) get Rhizoma Curcumae 10 grams by weight, add the water of Rhizoma Curcumae medical material 12 times amount, adopt extraction by steam distillation 2 hours, collect volatile oil;
(2) Radix Pseudostellariae 12 grams is got by weight, Radix Ophiopogonis 10 grams, Herba Hedyotidis Diffusae 20 grams, Herba Scutellariae Barbatae 20 grams, Pseudobulbus Cremastrae Seu Pleiones 15 grams, adds the Rhizoma Curcumae medicinal residues after step (1) vapor distillation, add the ethanol of total medical material weight 10 times of volumes, backflow or continuous circumfluence extraction 2 times, each 3 hours, merge extractive liquid, 50 ~ 80 degrees Celsius of lower concentrating under reduced pressure, macroporous adsorptive resins on concentrate, first washing with water, is then 20% ~ 70% ethanol elution by volumetric concentration successively; Collecting merging volumetric concentration is the ethanol elution of 55%, concentrates and to obtain extract;
(3) get the volatile oil that step (1) obtains, the extract that step (2) obtains adds lactose, and corn starch mix homogeneously, adds magnesium stearate lubricant, mix homogeneously, granulate, and then tablet made by tabletting.(lot number: 001)
Implementation column 2
1. have a Traditional Chinese medicine compound composition for anti-tumor activity, it is made up of the crude drug of following parts by weight: Radix Pseudostellariae 24 grams, Radix Ophiopogonis 20 grams, Herba Hedyotidis Diffusae 40 grams, Herba Scutellariae Barbatae 40 grams, Pseudobulbus Cremastrae Seu Pleiones 30 grams, Rhizoma Curcumae 20 grams.
2. the preparation method with the Traditional Chinese medicine compound composition of anti-tumor activity of the present invention, it comprises the following steps:
(1) get Rhizoma Curcumae 20 grams by weight, add the water of Rhizoma Curcumae medical material 10 times amount, adopt extraction by steam distillation 3 hours, collect volatile oil;
(2) Radix Pseudostellariae 24 grams is got by weight, Radix Ophiopogonis 20 grams, Herba Hedyotidis Diffusae 40 grams, Herba Scutellariae Barbatae 40 grams, Pseudobulbus Cremastrae Seu Pleiones 30 grams, adds the Rhizoma Curcumae medicinal residues after step (1) vapor distillation, add the ethanol of total medical material weight 12 times of volumes, backflow or continuous circumfluence extraction 3 times, each 2 hours, merge extractive liquid, 50 ~ 80 degrees Celsius of lower concentrating under reduced pressure, macroporous adsorptive resins on concentrate, first washing with water, is then 20% ~ 70% ethanol elution by volumetric concentration successively; Collecting merging volumetric concentration is the ethanol elution of 55%, concentrates and to obtain extract;
(3) get the volatile oil that step (1) obtains, the extract that step (2) obtains, then add diluent lactose or corn starch mix homogeneously, granulate, dry, make granule.(lot number: 002)
Implementation column 3
1. have a Traditional Chinese medicine compound composition for anti-tumor activity, it is made up of the crude drug of following parts by weight: Radix Pseudostellariae 36 grams, Radix Ophiopogonis 30 grams, Herba Hedyotidis Diffusae 60 grams, Herba Scutellariae Barbatae 60 grams, Pseudobulbus Cremastrae Seu Pleiones 45 grams, Rhizoma Curcumae 30 grams.
2. the preparation method with the Traditional Chinese medicine compound composition of anti-tumor activity of the present invention, it comprises the following steps:
(1) get Rhizoma Curcumae 30 grams by weight, add the water of Rhizoma Curcumae medical material 8 times amount, adopt extraction by steam distillation 3 hours, collect volatile oil;
(2) Radix Pseudostellariae 36 grams is got by weight, Radix Ophiopogonis 30 grams, Herba Hedyotidis Diffusae 60 grams, Herba Scutellariae Barbatae 60 grams, Pseudobulbus Cremastrae Seu Pleiones 45 grams, adds the Rhizoma Curcumae medicinal residues after step (1) vapor distillation, add the ethanol of total medical material weight 12 times of volumes, backflow or continuous circumfluence extraction 3 times, each 2 hours, merge extractive liquid, 50 ~ 80 degrees Celsius of lower concentrating under reduced pressure, macroporous adsorptive resins on concentrate, first washing with water, is then 20% ~ 70% ethanol elution by volumetric concentration successively; Collecting merging volumetric concentration is the ethanol elution of 55%, concentrates and to obtain extract;
(3) get the volatile oil that step (1) obtains, the extract that step (2) obtains, then and lactose, corn starch mixing all with, granulate, then encapsulatedly make capsule.(lot number: 003)
Implementation column 4 antitumor pharmacological evaluation
There is the Traditional Chinese medicine compound composition of anti-tumor activity to the experimentation of people's tumor inhibition effect in nude mouse
(1) to gastric carcinoma cells (MGC in nude mouse
-803) inhibitory action of cell
Experimental technique: by the MGC of adherent growth in Tissue Culture Flask
-803cell, is prepared into cell suspension, and adjustment cell concentration, counts under mirror, in every Mus right fore armpit subcutaneous vaccination 5 × 10
6individual MGC
-803cell, inoculates 24 altogether, it is divided at random treatment group (Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention, lot number 001, gastric infusion 2g/kg), and blank group (waiting capacity normal saline), often organizes 10.Inoculate after 48 hours, medication, continuous 21 days, after drug withdrawal, put to death animal and observe MGC
-803cell growing state claim tumor weight in nude mouse.Specific experiment the results are shown in Table 1.
MGC in table 1 pair nude mouse
-803the inhibitory action of cell
(2) to the inhibitory action of people's hepatocarcinoma (SMMC-7451) cell in nude mouse
Experimental technique: preparation SMMC-7451 cell suspension, adjustment cell concentration, every Mus right front axil subcutaneous vaccination SMMC-7451 cell 5 × 10
6individual/0.2ml, inoculates 20 altogether, is divided into the treatment group (Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention at random, lot number 002. gastric infusion, 2g/kg) and blank group (waiting capacity normal saline), often organize 10, after 24 hours, start medication, once a day, continuous 24 days, after drug withdrawal, put to death animal, observe tumor growth situation and take tumor tissue and weigh, specific experiment the results are shown in Table 2.
The inhibitory action of SMMC-7451 cell in table 2 pair nude mouse
(3) to the inhibitory action of people's casing slime adenocarcinoma in nude mouse
Get tumor source nude mice, take out tumor tissue, be cut into the fritter of 0.3-0.4mm, tumor tissue is seeded in the dorsal sc of nude mice, inoculate 20 altogether, in latter 24 days of inoculation, be divided into treatment group (Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention, lot number 003 at random, gastric infusion, 2g/kg) with blank group (waiting capacity normal saline), often organize 10, continuous use 21 days, after last administration, 24h puts to death animal, take tumor tissue to weigh, calculate suppression ratio (%), specific experiment the results are shown in Table 3.
The inhibitory action of people's casing slime adenocarcinoma in table 3 pair nude mouse
(4) to the inhibitory action of pulmonary carcinoma in nude mouse (MCF-7) cell
Experimental technique: preparation pulmonary carcinoma (MCF-7) cell suspension, adjustment cell concentration, every Mus right front axil subcutaneous vaccination MCF-7 cell 5 × 106/0.2ml, inoculate 20 altogether, be divided into the treatment group (Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention at random, lot number 001. gastric infusion, 2g/kg) and blank group (waiting capacity normal saline), often organize 10, after 24 hours, start medication, once a day, continuous 24 days, after drug withdrawal, put to death animal, observe tumor growth situation and take tumor tissue and weigh, specific experiment the results are shown in Table 4.
The inhibitory action of MCF-7 cell in table 4 pair nude mouse
Shown by above experimental result: the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention is to people's gastric cancer, hepatocarcinoma, people's casing slime adenocarcinoma, transplant in Pulmonary carcinoma nude mice body and there is obvious inhibitory action, show the effect in the Traditional Chinese medicine compound composition body with anti-tumor activity provided by the invention with very strong anti-human malignant tumor, may be used for the treatment of malignancy disease.
(5) In Vitro Anti human tongue carcinoma experiment
Cell strain: Human tongue cancer cell line is provided by Nanjing University of Traditional Chinese Medicine's pharmacological evaluation room; 96 orifice plates are purchased from Costar company; RMPI1640 culture medium is purchased from Gibco company; DMEM culture medium is purchased from Gibco company; New-born calf serum is purchased from Hangzhou Ilex purpurea Hassk.[I.chinensis Sims company; Tetrazolium bromide (MTT) and DMSO are purchased from Amresco company;
The experimental apparatus that following examples use is as follows:
Experimental apparatus: superclean bench (Suzhou purification model SW-CJ-IFD), CO
2incubator (SANYO model: XD-101), microplate reader BIO-RAD (Model NO.550Serial NO.16971).
The experimental technique that following examples use is as follows:
Experimental technique: use improvement mtt assay to carry out extracorporeal anti-tumor cell experiment to the Traditional Chinese medicine compound composition that the embodiment of the present invention 1 prepares: being digested by Human tongue cancer cell line (Tca-8113) pancreatin, count, making concentration is 5 × 10
4the cell suspension of individual/ml.Hole every in 96 orifice plates is added 100 μ l cell suspension (every holes 5 × 10
3individual cell), be then placed in 37 DEG C, 5%CO
2cultivate 24 hours in incubator; With non-fully culture medium dilution medicine to required different gradient concentrations, every hole adds the corresponding pastille culture medium of 100 μ L, sets up negative control group, Vehicle controls group and positive controls simultaneously, then 96 orifice plates is placed in 37 DEG C, 5%CO
2cultivate 72 hours in incubator.Then every hole adds 20 μ L MTT (5mg/ml), continues cultivation 4 hours, and stop cultivating, discard culture medium, every hole adds 150 μ L DMSO and dissolves, and shaking table mixes for 10 minutes gently.Under λ=4570nm, 620nm two wavelength, detect absorbance and the OD value in every hole by microplate reader, using the meansigma methods in each multiple hole as the OD value of this group cell, calculate the suppression ratio of each medicine.
The Traditional Chinese medicine compound composition that the experimental result display embodiment of the present invention 1 prepares, all have stronger inhibitory action to Human tongue cancer cell line, each dosage group presents good dose-effect relationship.Experimental result is as shown in table 5.
The exercising result of table 5 pair Human tongue cancer cell line
Implementation column 5 has the experiment of Traditional Chinese medicine compound composition to immunoregulation effect of anti-tumor activity
(1) on the impact of mice IgA, IgG, IgM content
Experimental technique: get ICR white mice 30, body weight 18 ~ 22g, be divided into Normal group at random, lotus tumor matched group and treatment group, traditional vaccination S180 sarcoma, inoculate latter 24 hours, treatment group gavages the present invention and only (2g/kg) has Traditional Chinese medicine compound composition (lot number 001) 0.3ml/ of anti-tumor activity, white mice eyeball blood sampling in the 15th day.Measure IgA, IgG, IgM content, specific experiment the results are shown in Table 6.
Table 6 is on the impact (mg/dl of mice IgA, IgG, IgM content
)
* P<0.01 (comparing with Normal group) * * P<0.05 (comparing with lotus tumor matched group)
Shown by the experimental result of above table 6: tumor-bearing mice Immunoglobulin IgA, IgG, IgM content are starkly lower than normal control mice; The Traditional Chinese medicine compound composition that the present invention has anti-tumor activity can improve low IgA, IgG, IgM content of tumor-bearing mice, show that the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention has immunologic enhancement, the immunologic function of tumor patient self can be improved, thus be conducive to improvement and self antitumor action of Quality of Life of Tumor Patients.
(2) on the impact of cAMP content in rat plasma
Experimental technique: get Wistar rat 45, body weight 150 ~ 180g, male and female dual-purpose, be divided into Normal group at random, lotus tumor matched group and treatment group, traditional vaccination S180 sarcoma, after 24 hours, treatment group gavages the Traditional Chinese medicine compound composition (lot number 002) that the present invention has anti-tumor activity, and 2ml/ only (2g/kg), continuous 14 days, Normal group such as to gavage at the capacity normal saline, the 15th day, and Rat Heart is taken a blood sample, measure cAMP content, the results are shown in Table 7.
Table 7 is on the impact of rat plasma cAMP content
* P<0.05 (comparing with lotus tumor matched group)
Shown by the experimental result of table 7: the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention, the cAMP content that tumor-bearing mice can be made low improves (* P<0.05), show that the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention has the effect significantly improving tumor patient immunologic function further, be of value to the rehabilitation of tumor disease.
(3) to the raising effect that tumor necrosis factor (INF) induces
Experimental technique: get Wistar rat 20, be divided into matched group and treatment group at random, every animal equal lumbar injection 10mg bacillus calmette-guerin vaccine, the 15th day intraperitoneal injection 80 μ gLPS, took a blood sample after 90 minutes.Treatment group gavages the present invention and only (lot number 001,2g/kg) has the Traditional Chinese medicine compound composition 2ml/ of anti-tumor activity, continuous 14 days, and matched group such as to gavage at the capacity normal saline.
Get test serum, be diluted to variable concentrations; Target cell L929 10% calf serum, RPM1-1640 culture fluid are adjusted to 2 × 106/ml cell, add in 96 porocyte culture plates; 100 μ l/ holes, then the test serum 100 μ l/ hole adding different extension rate; Put 37 DEG C, 5%CO
2cultivate 18 hours in incubator.Result judges: tire as INF with the maximum dilution multiple of 50% target cell death.Concrete outcome is in table 8.
The table 8 pair facilitation that rat INF induces
* P<0.001 (comparing with Normal group)
Shown by table 8 experimental result: the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention can obviously promote inducing of INF, (P<0.001), the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention is described by induced tumors necrosin INF to reach antineoplastic object.
Shown by above experimental result, the immunologic function of Traditional Chinese medicine compound composition to tumor patient with anti-tumor activity provided by the invention has raising effect, and the generation of cAMP content and promotion tumor necrosis factor (INF) in adjustable body, patient self anti-tumor function can be strengthened.
And the Traditional Chinese medicine compound composition clinical test results display with anti-tumor activity provided by the invention: to mammary cancer chemotherapy patient 60 example and Chemotherapy of Cervical Cancer patient 50 example, all take the Traditional Chinese medicine compound composition (embodiment of the present invention 1) with anti-tumor activity provided by the present invention, through clinical observation in 2 months, take the tumor patient of Traditional Chinese medicine compound composition provided by the invention, clinical symptoms tool has clear improvement, Life quality of lung cancer patients is improved, and the toxicity that chemotherapy causes obviously alleviates, show the effect that the Traditional Chinese medicine compound composition with anti-tumor activity provided by the present invention has protection to immunologic function and improves, and human peripheral blood resembles and has protective effect, clinic observation effective percentage is respectively 83.5% and 80.7%.
The present invention is according to Chinese medical theory, the theory of determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs is adopted to carry out proportioning prescription, shown by above experimental result, the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention has anticancer removing toxic substances, dissipating phlegm and removing blood stasis, supplementing QI and nourishing YIN effect, there is growth and the transferance of Tumor suppression, and the drug resistance of energy reversing tumor cell, impel the apoptosis of tumor cell, and without obvious toxic-side effects, illustrate that the Traditional Chinese medicine compound composition with anti-tumor activity provided by the invention may be used for preparing antineoplastic new medicine.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (8)
1. have a Traditional Chinese medicine compound composition for anti-tumor activity, it is characterized in that, it is made up of the crude drug of following parts by weight: Radix Pseudostellariae 12 ~ 36 parts, Radix Ophiopogonis 10 ~ 30 parts, Herba Hedyotidis Diffusae 20 ~ 60 parts, Herba Scutellariae Barbatae 20 ~ 60 parts, Pseudobulbus Cremastrae Seu Pleiones 15 ~ 45 parts, Rhizoma Curcumae 10 ~ 30 parts.
2. the Traditional Chinese medicine compound composition with anti-tumor activity according to claim 1, it is characterized in that, it is made up of the crude drug of following parts by weight: Radix Pseudostellariae 12 ~ 24 parts, Radix Ophiopogonis 10 ~ 20 parts, Herba Hedyotidis Diffusae 20 ~ 40 parts, Herba Scutellariae Barbatae 20 ~ 40 parts, Pseudobulbus Cremastrae Seu Pleiones 15 ~ 30 parts, Rhizoma Curcumae 10 ~ 20 parts.
3. the Traditional Chinese medicine compound composition with anti-tumor activity according to claim 2, is characterized in that, it is made up of the crude drug of following parts by weight: Radix Pseudostellariae 12 parts, Radix Ophiopogonis 10 parts, Herba Hedyotidis Diffusae 20 parts, Herba Scutellariae Barbatae 20 parts, Pseudobulbus Cremastrae Seu Pleiones 15 parts, Rhizoma Curcumae 10 parts.
4. the Traditional Chinese medicine compound composition with anti-tumor activity according to any one of claims 1 to 3, is characterized in that, the extract of each crude drug and pharmaceutically acceptable carrier is prepared into the medicine of oral liquid, granule, capsule or Tabules.
5. the preparation method with the Traditional Chinese medicine compound composition of anti-tumor activity described in any one of claims 1 to 3, is characterized in that comprising the following steps:
(1) get Rhizoma Curcumae by the parts by weight described in any one of claims 1 to 3, add the water of Rhizoma Curcumae medical material 8 ~ 16 times amount, adopt extraction by steam distillation 1 ~ 3 hour, collect volatile oil;
(2) Radix Pseudostellariae is got, Radix Ophiopogonis, Herba Hedyotidis Diffusae by the parts by weight described in any one of claims 1 to 3, Herba Scutellariae Barbatae and Pseudobulbus Cremastrae Seu Pleiones, add the Rhizoma Curcumae medicinal residues after step (1) vapor distillation, add the ethanol of total medical material weight 6 ~ 12 times of volumes, backflow or continuous circumfluence extraction 2 ~ 3 times, each 1 ~ 3 hour, merge extractive liquid, 50 ~ 80 degrees Celsius of lower concentrating under reduced pressure, obtain macroporous adsorptive resins on concentrate, first washing with water, is then 20% ~ 70% ethanol elution by volumetric concentration successively; Collecting merging volumetric concentration is the ethanol elution of 55%, concentrates and to obtain extract;
(3) get the volatile oil that step (1) obtains, the extract that step (2) obtains and pharmaceutically acceptable carrier are prepared into the medicine of oral liquid, granule, capsule or Tabules.
6. the preparation method with the Traditional Chinese medicine compound composition of anti-tumor activity according to claim 5, is characterized in that, the macroporous adsorbent resin described in step (1) is D101 resin.
7. the Traditional Chinese medicine compound composition with anti-tumor activity described in any one of claims 1 to 3 is preparing the application in antitumor drug.
8. the Traditional Chinese medicine compound composition with anti-tumor activity described in any one of claims 1 to 3 in the anti-pulmonary carcinoma of preparation, gastric cancer, hepatocarcinoma, colon cancer, cervical cancer, the application in breast carcinoma or Dendritic cell medicine.
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| CN105233173A (en) * | 2015-11-11 | 2016-01-13 | 梁占强 | Composition for treating tumors of livers, gall and pancreas and preparation method thereof |
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| CN105233173A (en) * | 2015-11-11 | 2016-01-13 | 梁占强 | Composition for treating tumors of livers, gall and pancreas and preparation method thereof |
| CN107233449A (en) * | 2017-07-28 | 2017-10-10 | 青海九康中药饮片有限公司 | A kind of Traditional Chinese medicine compound composition with antitumor activity and its preparation method and application |
| CN109331146A (en) * | 2018-11-23 | 2019-02-15 | 厦门市中医院 | A kind of herbal mixture for treating liver cancer |
| CN111759993A (en) * | 2020-08-25 | 2020-10-13 | 江西国光药业有限公司 | Traditional Chinese medicine composition for treating liver cancer and preparation method thereof |
| CN111759993B (en) * | 2020-08-25 | 2021-12-17 | 江西国光药业有限公司 | Traditional Chinese medicine composition for treating liver cancer and preparation method thereof |
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