CN104840748A - Traditional Chinese medicine composition capable of resisting brain cancer activity and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition capable of resisting brain cancer activity and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition capable of resisting brain cancer activity and a preparation method and an application thereof. The traditional Chinese medicine composition is prepared by 10 to 30 parts of giant typhonium rhizome, 15 to 45 parts of edible tulip, 3 to 9 parts of centipede, 10 to 30 parts of arisacma consanguineum, 10 to 30 parts of herba lycopi, and 10 to 30 parts of rhizome alismatis. After proofing by pharmacological experiments, the traditional Chinese medicine composition capable of resisting the brain cancer activity has the advantages that the proportion is scientific and reasonable, the usage of the medicine is strict, and the effects of resisting cancers and detoxifying, dissipating phlegm and removing blood stasis, and the like are realized; the strong killing function on the cells of brain cancer inside and outside the human body is realized, the generation of tumor necrosis factors is promoted, and the immunity of a tumor patient is improved; the safety is high, and the toxic and side effect is low. The preparation method has the advantages that the quality is stable, the content of extracted effective ingredients is high, the operability is high, and the preparation method is suitable for industrial and large-batch production.
Description
Technical field
The present invention relates to a kind of Chinese medicine, be specifically related to a kind of to there is Chinese medicine composition of anti-brain cancer activity and its preparation method and application.
Background technology
Intracranial tumor is also known as the cerebral tumor, and its unknown etiology as yet, tumor occurs from brain, meninges, and hypophysis cerebri, cranial nerve, cerebrovascular and embryonal rest organizer, be called Primary intracranial tumor.By the Malignant tumor of bonal metastasis of other organs and tissues of health to intracranial person, be called Secondary cases intracranial tumor.
Intracranial tumor prevalence global statistics is 40,/10 ten thousand.Illustrate that the sickness rate of intracranial tumor is not low, significant.With regard to the sickness rate of general tumour, cerebroma occupies the 5th (6.31%), only lower than stomach, uterus, mammary gland, esophageal tumor.Adult, cerebroma accounts for 2% of general tumour sum.If treat the drug main chemical drugs of the brain cancer clinically at present, but because chemotherapeutics toxicity is large, while killing tumor cell also non-selectivity kill the human autoimmune such as leukocyte, lymphocyte cell, cause patient usually due to because of the accompanying infection of autoimmune inferior capabilities, patient uses erious adverse reaction, and the course for the treatment of is long, costly, in Clinical practice, larger limitation is had.
Chinese medicine has unique advantage in treatment tumor, is that the natural class medicine of representative has the features such as toxic and side effects is little, action target spot is various, better tolerance with Chinese medicine, more and more comes into one's own in treatment tumor.But the anti-brain cancer drug medication amount of report is comparatively large at present, and impurity component is more, and onset is slower.
Therefore, research and develop a kind of anti-brain cancer effective, dosage is few, has good therapeutic effect to the brain cancer, and the low antineoplastic Chinese traditional medicine of untoward reaction is significant.
Summary of the invention
Goal of the invention: the object of the invention is to solve the deficiencies in the prior art, there is provided a kind of proportioning scientific and reasonable, determined curative effect, Clinical practice safety, the Chinese medicine composition of anti-brain cancer activity that what untoward reaction was low have, another object of the present invention is to provide this preparation method with the Chinese medicine composition of anti-brain cancer activity and the application in preparation treatment brain cancer disease medicament thereof.
Technical scheme: in order to realize above object, the technical scheme that the present invention takes is:
Have a Chinese medicine composition for anti-brain cancer activity, it is made up of the crude drug of following parts by weight:
Rhizoma Typhonii (processed) 10 ~ 30 parts, Pseudobulbus Cremastrae Seu Pleiones 15 ~ 45 parts, Scolopendra 3 ~ 9 parts, Rhizoma Arisaematis (processed) 10 ~ 30 parts, Herba Lycopi 10 ~ 30 parts, Rhizoma Alismatis 10 ~ 30 parts.
Preferably, the above-described Chinese medicine composition with anti-brain cancer activity, it is made up of the crude drug of following parts by weight: Rhizoma Typhonii (processed) 10 ~ 20 parts, Pseudobulbus Cremastrae Seu Pleiones 15 ~ 30 parts, Scolopendra 3 ~ 6 parts, Rhizoma Arisaematis (processed) 10 ~ 20 parts, Herba Lycopi 10 ~ 20 parts, Rhizoma Alismatis 10 ~ 20 parts.
Preferably, the above-described Chinese medicine composition with anti-brain cancer activity, it is made up of the crude drug of following parts by weight: Rhizoma Typhonii (processed) 10 parts, Pseudobulbus Cremastrae Seu Pleiones 15 parts, Scolopendra 3 parts, Rhizoma Arisaematis (processed) 10 parts, Herba Lycopi 10 parts, Rhizoma Alismatis 10 parts.
Preferably, the above-described Chinese medicine composition with anti-brain cancer activity, is prepared into the medicine of oral liquid, granule, capsule or Tabules by the extract of each crude drug and pharmaceutically acceptable carrier.
The preparation method with the Chinese medicine composition of anti-brain cancer activity of the present invention, it comprises the following steps:
(1) Rhizoma Typhonii (processed) is got by weight, Pseudobulbus Cremastrae Seu Pleiones, Rhizoma Arisaematis (processed), Herba Lycopi, Rhizoma Alismatis, adds the ethanol of total medical material weight 6 ~ 15 times of volumes, soak 24 ~ 48 hours, then ultrasonic, backflow or continuous circumfluence extraction 2 ~ 3 times, each 1 ~ 3 hour, merge extractive liquid, concentrated, macroporous resin column on concentrate, uses water successively, 20% ethanol ~ 60% ethanol elution, collect 40 ~ 50% ethanol elution, concentrated, obtain extract;
(2) get Scolopendra by weight, with petroleum ether continuous circumfluence extraction 2 ~ 3 times, each 1 ~ 3 hour, obtain petroleum ether extract, concentrate drying, obtain Scolopendra extract;
(3) get the extract that step (1) obtains, the Scolopendra extract that step (2) obtains and pharmaceutically acceptable carrier are prepared into the medicine of oral liquid, granule, capsule or Tabules.
The application of Chinese medicine composition in the anti-brain cancer medicine of preparation with anti-brain cancer activity of the present invention.
When the extract with each raw material of the Chinese medicine composition of anti-brain cancer activity provided by the invention is made oral liquid, the extract adding distil water of each raw material is dissolved, filter, add syrup, adjust ph, make oral liquid.
When the extract with each raw material of the Chinese medicine composition of anti-brain cancer activity provided by the invention is made tablet, the extract of each raw material and lactose or corn starch, add magnesium stearate lubricant when needing, mix homogeneously, granulate, then tablet made by tabletting.
The extract of each raw material and carrier lactose or corn starch mix homogeneously when the extract with each raw material of the Chinese medicine composition of anti-brain cancer activity provided by the invention makes capsule, granulate, then encapsulatedly makes capsule.
When the extract with each raw material of the Chinese medicine composition of anti-brain cancer activity provided by the invention makes granule, the extract of each raw material and diluent lactose or corn starch mix homogeneously, granulate, dry, make granule.
The Chinese medicine composition with anti-brain cancer activity provided by the invention, Rhizoma Typhonii (processed) is pungent, temperature; Poisonous.Return stomach, Liver Channel.Function with cure mainly: dispel the wind expectorant, and arresting convulsion is jerked, detoxicating and resolving stagnation of pathogens pain relieving.Stop up for middle wind-phlegm, facial hemiparalysis, syncope due to accumulation of phlegm is had a headache, migraine and general headache, sore throat pharyngalgia, tetanus; Venom etc.
Pseudobulbus Cremastrae Seu Pleiones nature and flavor: sweet in the mouth; Micro-pungent; Cold in nature; Return Liver Channel; Stomach warp; Lung meridian.There is heat-clearing and toxic substances removing; Dispersing swelling and dissipating binds effect, has treatment carbuncle malignant boil; Scrofula tuberculosis; Pharyngalgia sore throat effect.
Rhizoma Arisaematis (processed) is Rhizoma Arisaematis processed product, and bitter in the mouth is pungent, warm in nature, people's lung, liver, spleen channel, function: drying dampness to eliminate phlegm, expelling wind for relieving convulsion, dissolving lump and resolving mass, Repercusion analgesia.
Herba Lycopi's nature and flavor slightly warm in nature, bitter in the mouth, pungent.Function cures mainly blood circulation promoting and blood stasis dispelling, line water detumescence.For menoxenia, amenorrhea, dysmenorrhea, postnatal blood stasis stomachache,
Scolopendra nature and flavor: acrid in the mouth; Warm in nature; Poisonous.Gui Jing: Liver Channel.Effect: expelling wind and relieving convulsion; Removing obstruction in the collateral to relieve pain; Dispersing pathogen accumulation.Cure mainly: infantile convulsion, epilepsy; Spasm is twitched; Apoplexy; Tetanus; Rheumatoid arthritis stubborn; Migraine and general headache; Venom; Skin infection; Scrofula.
Rhizoma Alismatis nature and flavor: sweet, light, cold in nature; Gui Jing: return kidney channel, urinary bladder channel; Effect: promoting diuresis to eliminate damp pathogen, expel the heat-evil treating stranguria; Cure mainly: the puckery pain of dysuria, pyretic stranguria, edema distension, have loose bowels, dizziness due to fluid-retention, seminal emission.
All medicines share effects such as having good anticancer removing toxic substances, dissipating phlegm and removing blood stasis above, can be used for the treatment of the strongly fragrant poison of wind-phlegm, brain cancer disease caused by deficiency of both QI and YIN.
Beneficial effect: compared to the prior art the Chinese medicine composition with anti-brain cancer activity provided by the invention has the following advantages:
1, the present invention is according to Chinese medical theory, and adopt the theory of determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs to carry out Chinese prescription screening, the Chinese medicine composition proportioning with anti-brain cancer activity provided is scientific and reasonable, and medication is rigorous, has the effects such as good anticancer removing toxic substances, dissipating phlegm and removing blood stasis.Experimental result shows, the Chinese medicine composition with anti-brain cancer activity provided by the invention has well suppression brain cancer cell growth and can promote the apoptotic effect of brain tumor.
2, experimental result shows that the Chinese medicine composition with anti-brain cancer activity provided by the invention has the body resistance strengthening and constitution consolidating and immunologic function facilitation, play Mutiple Targets, the effect of multiple organ treatment tumor, tumor patient misery can be reduced, extend life cycle, improve the effect of life in patients, and show through long-term experiment research, the Chinese medicine composition safety with anti-brain cancer activity provided by the invention is high, and toxic and side effects is low.
3, the extract with the Chinese medicine composition of anti-brain cancer activity provided by the invention can facilitate and pharmaceutical carriers is prepared into multiple dosage form, taking convenience clinically; The preparation method with the Chinese medicine composition of anti-brain cancer activity provided by the invention simultaneously, workable, especially according to each herbal medicine effective ingredient chemical property design extraction and purification process, the extract active constituent content obtained is high, impurity is few, and have absorb fast, drug effect can be played rapidly, the advantages such as dosage is few clinically, and process stabilizing, be applicable to industrialized great production.
Detailed description of the invention:
The present invention is illustrated further below in conjunction with specific embodiment, these embodiments should be understood only be not used in for illustration of the present invention and limit the scope of the invention, after having read the present invention, the amendment of those skilled in the art to the various equivalent form of value of the present invention has all fallen within the application's claims limited range.
Implementation column 1
1. have a Chinese medicine composition for anti-brain cancer activity, it is made up of the crude drug of following parts by weight: Rhizoma Typhonii (processed) 10 grams, Pseudobulbus Cremastrae Seu Pleiones 15 grams, Scolopendra 3 grams, Rhizoma Arisaematis (processed) 10 grams, Herba Lycopi 10 grams, Rhizoma Alismatis 10 grams.
2. the preparation method with the Chinese medicine composition of anti-brain cancer activity of the present invention, it comprises the following steps:
(1) Rhizoma Typhonii (processed) 10 grams is got by weight, Pseudobulbus Cremastrae Seu Pleiones 15 grams, Rhizoma Arisaematis (processed) 10 grams, Herba Lycopi 10 grams, Rhizoma Alismatis 10 grams, adds the ethanol of total medical material weight 15 times of volumes, soak 48 hours, then ultrasonic, continuous circumfluence extraction 3 times, each 1 hour, merge extractive liquid, concentrated, macroporous resin column on concentrate, uses water successively, 20% ethanol ~ 60% ethanol elution, collect 40 ~ 50% ethanol elution, concentrated, obtain extract;
(2) get Scolopendra 3 grams by weight, with petroleum ether continuous circumfluence extraction 3 times, each 2 hours, obtain petroleum ether extract, concentrate drying, obtain Scolopendra extract;
(3) get the extract that step (1) obtains, the Scolopendra extract that step (2) obtains, add lactose, corn starch mix homogeneously, adds magnesium stearate lubricant, mix homogeneously, granulate, and then tablet made by tabletting.(lot number: 001)
Implementation column 2
1. have a Chinese medicine composition for anti-brain cancer activity, it is made up of the crude drug of following parts by weight: Rhizoma Typhonii (processed) 20 grams, Pseudobulbus Cremastrae Seu Pleiones 30 grams, Scolopendra 6 grams, Rhizoma Arisaematis (processed) 20 grams, Herba Lycopi 20 grams, Rhizoma Alismatis 20 grams.
2. the preparation method with the Chinese medicine composition of anti-brain cancer activity of the present invention, it comprises the following steps:
(1) Rhizoma Typhonii (processed) 20 grams is got by weight, Pseudobulbus Cremastrae Seu Pleiones 30 grams, Rhizoma Arisaematis (processed) 20 grams, Herba Lycopi 20 grams, Rhizoma Alismatis 20 grams, adds the ethanol of total medical material weight 10 times of volumes, soak 24 hours, then ultrasonic, supersound extraction 2 times, each 2 hours, merge extractive liquid, concentrated, macroporous resin column on concentrate, uses water successively, 20% ethanol ~ 60% ethanol elution, collect 40 ~ 50% ethanol elution, concentrated, obtain extract;
(2) get Scolopendra 6 grams by weight, with petroleum ether continuous circumfluence extraction 2 times, each 2 hours, obtain petroleum ether extract, concentrate drying, obtain Scolopendra extract;
(3) get the extract that step (1) obtains, the Scolopendra extract that step (2) obtains, then add diluent lactose or corn starch mix homogeneously, granulate, dry, make granule.(lot number: 002)
Implementation column 3
1. have a Chinese medicine composition for anti-brain cancer activity, it is made up of the crude drug of following parts by weight: Rhizoma Typhonii (processed) 30 grams, Pseudobulbus Cremastrae Seu Pleiones 45 grams, Scolopendra 9 grams, Rhizoma Arisaematis (processed) 30 grams, Herba Lycopi 30 grams, Rhizoma Alismatis 30 grams.
2. the preparation method with the Chinese medicine composition of anti-brain cancer activity of the present invention, it comprises the following steps:
(1) Rhizoma Typhonii (processed) 30 grams is got by weight, Pseudobulbus Cremastrae Seu Pleiones 45 grams, Rhizoma Arisaematis (processed) 30 grams, Herba Lycopi 30 grams, Rhizoma Alismatis 30 grams, adds the ethanol of total medical material weight 8 times of volumes, soak 48 hours, then ultrasonic, reflux, extract, 2 times, each 3 hours, merge extractive liquid, concentrated, macroporous resin column on concentrate, uses water successively, 20% ethanol ~ 60% ethanol elution, collect 40 ~ 50% ethanol elution, concentrated, obtain extract;
(2) get Scolopendra 6 grams by weight, with petroleum ether continuous circumfluence extraction 3 times, each 1 hour, obtain petroleum ether extract, concentrate drying, obtain Scolopendra extract;
(3) get the extract that step (1) obtains, the Scolopendra extract that step (2) obtains, then and lactose, corn starch mixing all with, granulate, then encapsulatedly make capsule.(lot number: 003)
Implementation column 4 active Immunotherapy of Cancer Induced
(1) to the inhibitory action of brain cancer cell strain GBM8401 in nude mouse
Experimental technique: prepare brain cancer cell strain GBM8401 cell suspension, adjustment cell concentration, every Mus right front axil subcutaneous vaccination FTC-133 cell 5 × 10
6individual/0.2ml, inoculates 24 altogether, is divided into the treatment group (Chinese medicine composition with anti-brain cancer activity that the embodiment of the present invention 1 provides at random, lot number 001. gastric infusion, 2g/kg) and blank group (waiting capacity normal saline), often organize 12, after 24 hours, start medication, once a day, continuous 24 days, after drug withdrawal, put to death animal, observe tumor growth situation and take tumor tissue and weigh, specific experiment the results are shown in Table 1.
The inhibitory action of brain cancer cell strain GBM8401 cell in table 1 pair nude mouse
(2) In Vitro Anti brain cancer active testing
Cell strain: brain cancer cell GBM8401; 96 orifice plates are purchased from Costar company; RMPI1640 culture medium is purchased from Gibco company; DMEM culture medium is purchased from Gibco company; New-born calf serum is purchased from Hangzhou Ilex purpurea Hassk.[I.chinensis Sims company; Tetrazolium bromide (MTT) and DMSO are purchased from Amresco company.
The experimental apparatus that following examples use is as follows:
Experimental apparatus: superclean bench (Suzhou purification model SW-CJ-IFD), CO
2incubator (SANYO model: XD-101), microplate reader BIO-RAD (Model NO.550Serial NO.16971).
The experimental technique that following examples use is as follows:
Experimental technique: use improvement mtt assay to carry out In Vitro Anti human brain cancer cells experiment to the Chinese medicine composition that the embodiment of the present invention 1 prepares: being digested by brain cancer cell GBM8401 pancreatin, count, making concentration is 5 × 10
4the cell suspension of individual/ml.Hole every in 96 orifice plates is added 100 μ l cell suspension (every holes 5 × 10
3individual cell), be then placed in 37 DEG C, 5%CO
2cultivate 24 hours in incubator; With non-fully culture medium dilution medicine to required different gradient concentrations, every hole adds the corresponding pastille culture medium of 100 μ L, sets up negative control group, Vehicle controls group and positive controls simultaneously, then 96 orifice plates is placed in 37 DEG C, 5%CO
2cultivate 72 hours in incubator.Then every hole adds 20 μ L MTT (5mg/ml), continues cultivation 4 hours, and stop cultivating, discard culture medium, every hole adds 150 μ L DMSO and dissolves, and shaking table mixes for 10 minutes gently.Under λ=4570nm, 620nm two wavelength, detect absorbance and the OD value in every hole by microplate reader, using the meansigma methods in each multiple hole as the OD value of this group cell, calculate the suppression ratio of each medicine.
The Chinese medicine composition that the experimental result display embodiment of the present invention 1 prepares, have stronger inhibitory action to human brain cancer cells GBM8401, each dosage group presents good dose-effect relationship.Experimental result is as shown in table 2.
The inhibitory action result of table 2 couple human brain cancer cells GBM8401
Shown by above experimental result: the Chinese medicine composition with anti-brain cancer activity provided by the invention has obvious inhibitory action to transplanting in people's brain cancer nude mouse, the external increment that also obviously can suppress brain cancer cell simultaneously, show in the Chinese medicine composition object with anti-brain cancer activity provided by the invention and the external effect all with the very strong anti-brain cancer, may be used for the treatment of brain cancer disease.Implementation column 5 has the Chinese medicine composition of anti-brain cancer activity to the experiment of immunoregulation effect
(1) on the impact of mice IgA, IgG, IgM content
Experimental technique: get ICR white mice 30, body weight 18 ~ 22g, be divided into Normal group at random, lotus tumor matched group and treatment group, traditional vaccination brain cancer cell, inoculate latter 24 hours, treatment group gavages Chinese medicine composition (lot number 001) 0.3ml/ with anti-brain cancer activity (2g/kg) that the embodiment of the present invention 1 prepares, white mice eyeball blood sampling in the 15th day.Measure IgA, IgG, IgM content, specific experiment the results are shown in Table 3.
Table 3 is on the impact (mg/dl of mice IgA, IgG, IgM content
)
* P<0.01 (comparing with Normal group) * * P<0.05 (comparing with lotus tumor matched group)
Shown by the experimental result of above table 3: the mice of the inoculation brain cancer, Immunoglobulin IgA, IgG, IgM content are starkly lower than normal control mice; The Chinese medicine composition that the present invention has an anti-brain cancer activity can significantly improve IgA, IgG, IgM content of mice, show that the Chinese medicine composition with anti-brain cancer activity provided by the invention has stronger immunologic enhancement, the immunologic function of tumor patient self can be improved, thus be conducive to improving Quality of Life of Tumor Patients and promoting self antitumor action.
(2) to the raising effect that tumor necrosis factor (INF) induces
Experimental technique: get Wistar rat 20, be divided into matched group and treatment group at random, every animal equal lumbar injection 10mg bacillus calmette-guerin vaccine, the 15th day intraperitoneal injection 80 μ gLPS, took a blood sample after 90 minutes.Treatment group gavages the embodiment of the present invention 1 and only (lot number 001,2g/kg) has the Chinese medicine composition 2ml/ of anti-brain cancer activity, continuous 14 days, and matched group such as to gavage at the capacity normal saline.
Get test serum, be diluted to variable concentrations; Target cell L929 10% calf serum, RPM1-1640 culture fluid are adjusted to 2 × 10
6/ ml cell, adds in 96 porocyte culture plates; 100 μ l/ holes, then the test serum 100 μ l/ hole adding different extension rate; Put 37 DEG C, 5%CO
2cultivate 18 hours in incubator.Result judges: tire as INF with the maximum dilution multiple of 50% target cell death.Concrete outcome is in table 4.
The table 4 pair facilitation that rat INF induces
* P<0.001 (comparing with Normal group)
Shown by table 4 experimental result: the Chinese medicine composition with anti-brain cancer activity provided by the invention can obviously promote inducing of INF, (P<0.001), the Chinese medicine composition with anti-brain cancer activity provided by the invention is described by induced tumors necrosin INF to reach antineoplastic object.
Shown by above experimental result, the immunologic function of Chinese medicine composition to tumor patient with anti-brain cancer activity provided by the invention has raising effect, and can promote the generation of tumor necrosis factor (INF), can strengthen patient self anti-tumor function.
The present invention is according to Chinese medical theory, the theory of determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs is adopted to carry out proportioning prescription, medication is rigorous, shown by above experimental result, the Chinese medicine composition with anti-brain cancer activity provided by the invention has the effects such as anticancer removing toxic substances, eleminating phlegm and freeing channels, there is the growth and transferance that suppress brain cancer tumor, and without obvious toxic-side effects, illustrate that the Chinese medicine composition with anti-brain cancer activity provided by the invention may be used for preparing the novel drugs of the anti-brain cancer.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. have a Chinese medicine composition for anti-brain cancer activity, it is characterized in that, it is made up of the crude drug of following parts by weight:
Rhizoma Typhonii (processed) 10 ~ 30 parts, Pseudobulbus Cremastrae Seu Pleiones 15 ~ 45 parts, Scolopendra 3 ~ 9 parts, Rhizoma Arisaematis (processed) 10 ~ 30 parts, Herba Lycopi 10 ~ 30 parts, Rhizoma Alismatis 10 ~ 30 parts.
2. the Chinese medicine composition with anti-brain cancer activity according to claim 1, it is characterized in that, it is made up of the crude drug of following parts by weight: Rhizoma Typhonii (processed) 10 ~ 20 parts, Pseudobulbus Cremastrae Seu Pleiones 15 ~ 30 parts, Scolopendra 3 ~ 6 parts, Rhizoma Arisaematis (processed) 10 ~ 20 parts, Herba Lycopi 10 ~ 20 parts, Rhizoma Alismatis 10 ~ 20 parts.
3. the Chinese medicine composition with anti-brain cancer activity according to claim 2, it is characterized in that, it is made up of the crude drug of following parts by weight: Rhizoma Typhonii (processed) 10 parts, Pseudobulbus Cremastrae Seu Pleiones 15 parts, Scolopendra 3 parts, Rhizoma Arisaematis (processed) 10 parts, Herba Lycopi 10 parts, Rhizoma Alismatis 10 parts.
4. the Chinese medicine composition with anti-brain cancer activity according to any one of claims 1 to 3, is characterized in that, the extract of each crude drug and pharmaceutically acceptable carrier is prepared into the medicine of oral liquid, granule, capsule or Tabules.
5. the preparation method with the Chinese medicine composition of anti-brain cancer activity described in any one of claims 1 to 3, is characterized in that comprising the following steps:
(1) Rhizoma Typhonii (processed) is got, Pseudobulbus Cremastrae Seu Pleiones, Rhizoma Arisaematis (processed), Herba Lycopi, Rhizoma Alismatis by the parts by weight described in any one of claims 1 to 3,
, add the ethanol of total medical material weight 6 ~ 15 times of volumes, soak 24 ~ 48 hours, then ultrasonic, backflow or continuous circumfluence extraction 2 ~ 3 times, each 1 ~ 3 hour, merge extractive liquid, concentrated, macroporous resin column on concentrate, uses water successively, 20% ethanol ~ 60% ethanol elution, collect 40 ~ 50% ethanol elution, concentrated, obtain extract;
(2) get Scolopendra by the parts by weight described in any one of claims 1 to 3, with petroleum ether continuous circumfluence extraction 2 ~ 3 times, each 1 ~ 3 hour, obtain petroleum ether extract, concentrate drying, obtain Scolopendra extract;
(3) get the extract that step (1) obtains, the Scolopendra extract that step (2) obtains and pharmaceutically acceptable carrier are prepared into the medicine of oral liquid, granule, capsule or Tabules.
6. the preparation method with the Chinese medicine composition of anti-brain cancer activity according to claim 5, is characterized in that, the macroporous resin described in step (2) is D101 type resin.
7. the application of the Chinese medicine composition with anti-brain cancer activity described in any one of claims 1 to 3 in the anti-brain cancer medicine of preparation.
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| CN113288934A (en) * | 2020-03-25 | 2021-08-24 | 厦门大学 | Herba lycopi extract, preparation method and application thereof |
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| CN1246358A (en) * | 1999-08-10 | 2000-03-08 | 辛长山 | Medical capsule for treating cerebroma and its preparing process |
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2015
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1246358A (en) * | 1999-08-10 | 2000-03-08 | 辛长山 | Medical capsule for treating cerebroma and its preparing process |
Non-Patent Citations (1)
| Title |
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| 霍介格等: "周仲瑛***的临证思路探析", 《上海中医药杂志》 * |
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| CN113288934A (en) * | 2020-03-25 | 2021-08-24 | 厦门大学 | Herba lycopi extract, preparation method and application thereof |
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