CN104857435B - A kind of Traditional Chinese medicine compound composition with antitumor activity and its preparation method and application - Google Patents
A kind of Traditional Chinese medicine compound composition with antitumor activity and its preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of Traditional Chinese medicine compound compositions with antitumor activity and preparation method thereof and its application, the Traditional Chinese medicine compound composition is by 12~36 parts of radix pseudostellariae, 10~30 parts of Radix Ophiopogonis, 20~60 parts of oldenlandia diffusa, 20~60 parts of Sculellaria barbata, 10~30 parts of 15~45 parts of edible tulip and curcuma zedoary are made.Show through pharmacological evaluation and clinical trial, Traditional Chinese medicine compound composition provided by the invention with antitumor activity, proportioning is scientific and reasonable, has anticancer removing toxic substances, dissipating phlegm and removing blood stasis, supplementing qi and nourishing yin and other effects, strong killing effect is respectively provided with to Several Kinds of Malignancy cell, is had wide range of applications；And cAMP contents and promotion tumor necrosis factor in adjustable body（INF）Generation, improve tumor patient immunity, extend life quality, and safe, toxic side effect is low.Preparation method provided by the invention, stable quality, effective component extracting content is high, and operability is strong, is suitble to industrialized production.
Description
Technical field
The present invention relates to a kind of Chinese medicines, and in particular to a kind of Traditional Chinese medicine compound composition and its preparation with antitumor activity
Methods and applications.
Background technology
Tumour has become the number one killer for 21 century threatening human survival, has more than 600 ten thousand people to die of every year on average various swollen
Knurl disease.China is one of world's cancer country occurred frequently, and is presented a rapidly rising trend every year.Tumour is clinically treated at present
Drug mainly has chemicals, but since chemotherapeutics toxicity is big, the also kill of non-selectivity while tumour cell is killed
Human autoimmunes' cell such as leucocyte, lymphocyte, cause patient usually due to because autoimmunity inferior capabilities be complicated by infection,
Patient uses erious adverse reaction, and the course for the treatment of is long, costly, there is larger limitation in Clinical practice.
Traditional Chinese medicine has unique advantage in terms of tumour is treated, and the natural class drug using Chinese medicine as representative has poison is secondary to make
With the features such as small, action target spot is various, tolerance is good, more and more it is taken seriously in terms of tumour is treated.But report at present
Antitumor drug dosage is larger, and impurity component is more.
Therefore, it is good to research and develop a kind of antitumous effect, dosage is few, and treatment effect well is respectively provided with to kinds of tumors
Fruit, and the low antineoplastic Chinese traditional medicine of adverse reaction is of great significance.
Invention content
Goal of the invention：Present invention aims to solve the deficiencies of the prior art, and provides a kind of it is a kind of proportioning it is scientific and reasonable, curative effect is true
It cuts, Clinical practice safety, the low Traditional Chinese medicine compound composition with antitumor activity of adverse reaction, another object of the present invention is
The preparation method of the Traditional Chinese medicine compound composition with antitumor activity is provided and its treats malignancy disease drug preparing
In application.
Technical solution：In order to achieve the goal above, the technical solution that the present invention takes is：
A kind of Traditional Chinese medicine compound composition with antitumor activity, it is made of the bulk pharmaceutical chemicals of following parts by weight：Crown prince
12~36 parts of ginseng, 10~30 parts of Radix Ophiopogonis, 20~60 parts of oldenlandia diffusa, 20~60 parts of Sculellaria barbata, 15~45 parts of edible tulip, curcuma zedoary
10~30 parts.
Preferably, the above-described Traditional Chinese medicine compound composition with antitumor activity, it is by following parts by weight
Several bulk pharmaceutical chemicals are made：12~24 parts of radix pseudostellariae, 10~20 parts of Radix Ophiopogonis, 20~40 parts of oldenlandia diffusa, 20~40 parts of Sculellaria barbata,
15~30 parts of edible tulip, 10~20 parts of curcuma zedoary.
As more preferred scheme, the above-described Traditional Chinese medicine compound composition with antitumor activity, it is by following heavy
The bulk pharmaceutical chemicals of amount number are made：12 parts of radix pseudostellariae, 10 parts of Radix Ophiopogonis, 20 parts of oldenlandia diffusa, 20 parts of Sculellaria barbata, 15 parts of edible tulip,
10 parts of curcuma zedoary.
Traditional Chinese medicine compound composition of the present invention with antitumor activity, by the extract of each bulk pharmaceutical chemicals and pharmaceutically
Acceptable carrier is prepared into the drug of oral liquid, granule, capsule or Tabules.
The preparation method of Traditional Chinese medicine compound composition of the present invention with antitumor activity, includes the following steps：
(1) take curcuma zedoary in parts by weight, add in the water of 8~16 times of amounts of curcuma zedoary medicinal material, using extraction by steam distillation 1~
3 hours, collect volatile oil；
(2) radix pseudostellariae, Radix Ophiopogonis, oldenlandia diffusa, Sculellaria barbata and edible tulip are taken in parts by weight, are added in step (1) water and are steamed
The curcuma zedoary dregs of a decoction after steam distillation add in the ethyl alcohol of total 6~12 times of volumes of medicinal material weight, reflux or continuous circumfluence extraction 2~3
It is secondary, 1~3 hour every time, merge extracting solution, be concentrated under reduced pressure under 50~80 degrees Celsius, obtain large pore resin absorption column on concentrate, first
It is eluted with water, it is 20%~70% ethanol elution then to use volumetric concentration successively；Collect the ethyl alcohol for merging that volumetric concentration is 55%
Eluent is concentrated to give extract；
(3) it is prepared by the volatile oil that step (1) is taken to obtain, the extract and pharmaceutically acceptable carrier that step (2) obtains
Into the drug of oral liquid, granule, capsule or Tabules.
The extract of each raw material of Traditional Chinese medicine compound composition provided by the invention with antitumor activity is made oral
During liquid, the extract of each raw material is added into distillation water dissolution, is filtered, adds in syrup, pH value is adjusted, oral liquid is made.
Tablet is made in the extract of each raw material of Traditional Chinese medicine compound composition provided by the invention with antitumor activity
When, the extract and lactose or cornstarch of each raw material, magnesium stearate lubricant is added in when needing, is uniformly mixed, whole grain, so
Tablet is made in tabletting afterwards.
Capsule is made in the extract of each raw material of Traditional Chinese medicine compound composition provided by the invention with antitumor activity
When the extract and carrier lactose or cornstarch of each raw material are uniformly mixed, whole grain is then encapsulated that capsule is made.
Granule is made in the extract of each raw material of Traditional Chinese medicine compound composition provided by the invention with antitumor activity
When, the extract and diluent lactose or cornstarch of each raw material are uniformly mixed, whole grain, it is dry, granule is made.
Preferably, the preparation method of the above-described Traditional Chinese medicine compound composition with antitumor activity, step
(1) macroporous absorbent resin described in is D101 resins.
Traditional Chinese medicine compound composition application in preparation of anti-tumor drugs of the present invention with antitumor activity.
Preferably, the Traditional Chinese medicine compound composition provided by the invention with antitumor activity is preparing anti-lung cancer,
Application in gastric cancer, liver cancer, colon cancer, breast cancer, cervical carcinoma or human tongue carcinoma drug.
Chinese medicine composition provided by the invention with anti-lung cancer activity, radix pseudostellariae nature and flavor：Sweet, slight bitter；Cold nature., return
Through：The spleen channel, lung channel.Effect：Help spleen and lung；Nourishing generate fluid.It cures mainly：It is weakness of the spleen and the stomach；Loss of appetite；Lassitude；Impairment of both QI and YIN；
Dry cough phlegm is few and the convalescent stage of febrile disease deficiency of vital energy Tianjin is hindered；Interior heat is thirsty.
Radix Ophiopogonis sweet slight bitter trembles with fear, enters lung channel, stomach, the heart channel of Hang-Shaoyin.With Yin nourishing and lung moistening, relieving restlessness that clears away heart-fire, reinforcing stomach reg fluid effect, tool
There is treatment dryness of the lung dry cough, haematemesis is spat blood, consumptive lung disease, lung carbuncle, and consumptive disease dysphoria with smothery sensation is quenched one's thirst, pyreticosis Tianjin wound, dry throat, constipation effect.
Oldenlandia diffusa bitter sweet is trembled with fear.Enter the heart channel of Hang-Shaoyin, Liver Channel, the spleen channel.With clearing heat and detoxicating；Dampness removing acts on, and has treatment lung heat
It breathes with cough；Abscess of throat；Acute appendicitis；Furuncle sore；Venomous snake bite；Puckery pain of heat gonorrhea；Oedema；Dysentery；Enteritis；Jaundice with damp-heat pathogen；Cancerous swelling work(
Effect.
Edible tulip nature and flavor：It is sweet in flavor；Micro-pungent；It is cold in nature；Return liver warp；Stomach；Lung channel.With clearing heat and detoxicating；Dispersing swelling and dissipating binds act on,
With treatment ulcer malignant sore；Scrofula tuberculosis；Pharyngalgia larynx numbness effect.
Sculellaria barbata nature and flavor：Acrid flavour；It is bitter；It is cold in nature.Effect：It is clearing heat and detoxicating；Dissipate stasis of blood hemostasis；Inducing diuresis to remove edema.It cures mainly：Heat toxin carbuncle
It is swollen；It has sore throat；Lung carbuncle；Acute appendicitis；Scrofula；Venomous snake bite；Traumatic injury；It spits blood；Bleeding from five sense organs or subcutaneous tissue；Blood strangury；Oedema；Ascites and cancer.
Curcuma zedoary acrid flavour, hardship, temperature.Return liver, the spleen channel.With blood-breaking, Xiao Ji Zhi Tong, dispelling wind and eliminating dampness, swelling and pain relieving effect.
For rheumatalgia, intermittent headache pain, chest and hypochondrium, diarrhea pain；Traumatic injury, blood stasis and swelling pain treatment are controlled in external application.
More than all medicines share has good anticancer removing toxic substances, dissipating phlegm and removing blood stasis, supplementing qi and nourishing yin and other effects, has and inhibits well
Growth of tumour cell and the effect that apoptosis of tumor cells can be promoted.
Advantageous effect：Traditional Chinese medicine compound composition provided by the invention with antitumor activity compared to the prior art with
Advantages below：
1st, the present invention is according to traditional Chinese medical theory, and theoretical using diagnosis and treatment carries out Chinese prescription screening, and what is provided has
The Traditional Chinese medicine compound composition proportioning of antitumor activity is scientific and reasonable, has good anticancer removing toxic substances, dissipating phlegm and removing blood stasis, supplementing qi and nourishing yin etc.
Effect.The experimental results showed that the Traditional Chinese medicine compound composition provided by the invention with antitumor activity inhibits swollen with good
Tumor cell growth and the effect that apoptosis of tumor cells can be promoted, and strong killing is respectively provided with to Several Kinds of Malignancy cell and is made
With having wide range of applications.
2nd, the experimental results showed that the Traditional Chinese medicine compound composition provided by the invention with antitumor activity is with righting reinforcing
With immune function facilitation, and in adjustable body cAMP contents and promote tumor necrosis factor (INF) generation, play more targets
Point, multiple organ treat the effect of tumour, can reduce tumor patient pain, extend life cycle, improve the effect of life in patients,
And through long-term experiment research shows that, the Traditional Chinese medicine compound composition provided by the invention with antitumor activity is safe, and poison is secondary
It acts on low.
3rd, the extract of the Traditional Chinese medicine compound composition provided by the invention with antitumor activity can be carried conveniently with pharmacy
Body is prepared into a variety of dosage forms, clinically convenient to take；Chinese medicine compound prescription combination provided by the invention simultaneously with antitumor activity
The preparation method of object, operability is strong, designs extraction and purification process especially in accordance with each herbal medicine active ingredient chemical property, obtains
The extract active constituent content that arrives is high, and impurity is few, and with absorb it is fast, drug effect can be played rapidly, clinically dosage is few etc.
Advantage, and process stabilizing are suitble to industrialized production.
Specific embodiment：
With reference to specific embodiment, the present invention is furture elucidated, it should be understood that these embodiments be merely to illustrate the present invention and
It is not used in and limits the scope of the invention, after the present invention has been read, those skilled in the art are to the various shapes of equal value of the present invention
The modification of formula falls within the application range as defined in the appended claims.
Implementation column 1
1. a kind of Traditional Chinese medicine compound composition with antitumor activity, it is made of the bulk pharmaceutical chemicals of following parts by weight：Too
12 grams of son ginseng, 10 grams of Radix Ophiopogonis, 20 grams of oldenlandia diffusa, 20 grams of Sculellaria barbata, 15 grams of edible tulip, 10 grams of curcuma zedoary.
2. the preparation method of the Traditional Chinese medicine compound composition of the present invention with antitumor activity, including following step
Suddenly：
(1) 10 grams of curcuma zedoary is taken in parts by weight, the water of 12 times of amounts of curcuma zedoary medicinal material is added in, using extraction by steam distillation 2
Hour, collect volatile oil；
(2) 12 grams of radix pseudostellariae, 10 grams of Radix Ophiopogonis, 20 grams of oldenlandia diffusa, 20 grams of Sculellaria barbata, edible tulip 15 are taken in parts by weight
Gram, add in the curcuma zedoary dregs of a decoction after step (1) steam distillation, add in the ethyl alcohol of total 10 times of volumes of medicinal material weight, reflux or
Continuous circumfluence extraction 2 times 3 hours every time, merges extracting solution, is concentrated under reduced pressure under 50~80 degrees Celsius, obtains macropore on concentrate and inhales
Attached resin column, is first eluted with water, and it is 20%~70% ethanol elution then to use volumetric concentration successively；Collecting merging volumetric concentration is
55% ethanol eluate, is concentrated to give extract；
(3) volatile oil that step (1) is taken to obtain, the extract that step (2) obtains add in lactose, and cornstarch mixing is equal
It is even, magnesium stearate lubricant is added in, is uniformly mixed, tablet is made in whole grain, then tabletting.(lot number：001)
Implementation column 2
1. a kind of Traditional Chinese medicine compound composition with antitumor activity, it is made of the bulk pharmaceutical chemicals of following parts by weight：Too
24 grams of son ginseng, 20 grams of Radix Ophiopogonis, 40 grams of oldenlandia diffusa, 40 grams of Sculellaria barbata, 30 grams of edible tulip, 20 grams of curcuma zedoary.
2. the preparation method of the Traditional Chinese medicine compound composition of the present invention with antitumor activity, including following step
Suddenly：
(1) 20 grams of curcuma zedoary is taken in parts by weight, the water of 10 times of amounts of curcuma zedoary medicinal material is added in, using extraction by steam distillation 3
Hour, collect volatile oil；
(2) 24 grams of radix pseudostellariae, 20 grams of Radix Ophiopogonis, 40 grams of oldenlandia diffusa, 40 grams of Sculellaria barbata, edible tulip 30 are taken in parts by weight
Gram, add in the curcuma zedoary dregs of a decoction after step (1) steam distillation, add in the ethyl alcohol of total 12 times of volumes of medicinal material weight, reflux or
Continuous circumfluence extraction 3 times 2 hours every time, merges extracting solution, is concentrated under reduced pressure under 50~80 degrees Celsius, obtains macropore on concentrate and inhales
Attached resin column, is first eluted with water, and it is 20%~70% ethanol elution then to use volumetric concentration successively；Collecting merging volumetric concentration is
55% ethanol eluate, is concentrated to give extract；
(3) then the volatile oil that step (1) is taken to obtain, the extract that step (2) obtains add in diluent lactose or corn
Starch is uniformly mixed, whole grain, dry, and granule is made.(lot number：002)
Implementation column 3
1. a kind of Traditional Chinese medicine compound composition with antitumor activity, it is made of the bulk pharmaceutical chemicals of following parts by weight：Too
36 grams of son ginseng, 30 grams of Radix Ophiopogonis, 60 grams of oldenlandia diffusa, 60 grams of Sculellaria barbata, 45 grams of edible tulip, 30 grams of curcuma zedoary.
2. the preparation method of the Traditional Chinese medicine compound composition of the present invention with antitumor activity, including following step
Suddenly：
(1) 30 grams of curcuma zedoary is taken in parts by weight, adds in the water of 8 times of amounts of curcuma zedoary medicinal material, it is small using extraction by steam distillation 3
When, collect volatile oil；
(2) 36 grams of radix pseudostellariae, 30 grams of Radix Ophiopogonis, 60 grams of oldenlandia diffusa, 60 grams of Sculellaria barbata, edible tulip 45 are taken in parts by weight
Gram, add in the curcuma zedoary dregs of a decoction after step (1) steam distillation, add in the ethyl alcohol of total 12 times of volumes of medicinal material weight, reflux or
Continuous circumfluence extraction 3 times 2 hours every time, merges extracting solution, is concentrated under reduced pressure under 50~80 degrees Celsius, obtains macropore on concentrate and inhales
Attached resin column, is first eluted with water, and it is 20%~70% ethanol elution then to use volumetric concentration successively；Collecting merging volumetric concentration is
55% ethanol eluate, is concentrated to give extract；
(3) then the volatile oil that step (1) is taken to obtain, the extract that step (2) obtains are mixed with lactose, cornstarch
With whole grain is then encapsulated that capsule is made.(lot number：003)
4 antitumor pharmacological evaluation of implementation column
Traditional Chinese medicine compound composition with antitumor activity is to the experimental study of human tumour inhibiting effect in nude mouse
(1) to gastric carcinoma cells (MGC in nude mouse-803) cell inhibiting effect
Experimental method：By the MGC of adherent growth in Tissue Culture Flask-803Cell, is prepared into cell suspension, and adjustment cell is dense
It spends, is counted under mirror, 5 × 10 are inoculated in every mouse right fore armpit6A MGC-803Cell is inoculated with 24, is randomly divided into altogether
Treatment group (Traditional Chinese medicine compound composition provided by the invention with antitumor activity, lot number 001, gastric infusion 2g/kg) and sky
White group (waiting capacity physiological saline), every group 10.After inoculation 48 hours, medication continuous 21 days, after drug withdrawal, puts to death animal observation
MGC-803Cell growing state and claims knurl weight in nude mouse.Specific experiment the results are shown in Table 1.
Table 1 is to MGC in nude mouse-803The inhibiting effect of cell
(2) to the inhibiting effect of human liver cancer in nude mouse (SMMC-7451) cell
Experimental method：SMMC-7451 cell suspensions are prepared, adjust cell concentration, armpit inoculates SMMC- before every mouse right side
7451 cells 5 × 106A/0.2ml is inoculated with 20 altogether, and it is (provided by the invention with antitumor activity to be randomly divided into treatment group
Traditional Chinese medicine compound composition, 002. gastric infusion of lot number, 2g/kg) and blank group (waiting capacity physiological saline), every group 10,24 is small
Shi Hou is started medication, and once a day, continuous 24 days, after drug withdrawal, is put to death animal, is observed tumour growth situation and take tumor tissue
It weighs, specific experiment the results are shown in Table 2.
Table 2 is to the inhibiting effect of SMMC-7451 cells in nude mouse
(3) to the inhibiting effect of people's casing slime gland cancer in nude mouse
Knurl source nude mice is taken, takes out tumor tissue, is cut into the fritter of 0.3-0.4mm, tumor tissue is seeded in the back skin of nude mice
Under, it is inoculated with 20 altogether, 24 days after inoculation, being randomly divided into treatment group, (Chinese medicine provided by the invention with antitumor activity is answered
Square composition, lot number 003, gastric infusion, 2g/kg) and blank group (waiting capacity physiological saline), every group 10, continuous use 21
My god, it in putting to death animal for 24 hours after the last administration, takes tumor tissue and weighs, calculate inhibiting rate (%), specific experiment the results are shown in Table 3.
Table 3 is to the inhibiting effect of people's casing slime gland cancer in nude mouse
(4) to the inhibiting effect of lung cancer in nude mouse (MCF-7) cell
Experimental method：Lung cancer (MCF-7) cell suspension is prepared, adjusts cell concentration, armpit inoculates MCF-7 before every mouse right side
5 × 106/0.2ml of cell is inoculated with 20, is randomly divided into treatment group's (Chinese medicine provided by the invention with antitumor activity altogether
Compound, 001. gastric infusion of lot number, 2g/kg) and blank group (waiting capacity physiological saline), after every group 10,24 hours,
It starts medication, once a day, continuous 24 days, after drug withdrawal, puts to death animal, observe tumour growth situation and take tumor tissue and weigh,
Specific experiment the results are shown in Table 4.
Table 4 is to the inhibiting effect of MCF-7 cells in nude mouse
By above the experimental results showed that：Traditional Chinese medicine compound composition provided by the invention with antitumor activity is to people's stomach
Cancer, liver cancer, people's casing slime gland cancer, in Pulmonary carcinoma nude mice body transplanting significantly inhibit, show provided by the invention have
There is very strong anti-human malignant tumour in the Traditional Chinese medicine compound composition body of antitumor activity, can be used for malignant tumour disease
The treatment of disease.
(5) external anti-human Dendritic cell experiment
Cell strain：Human tongue cancer cell line is provided by Nanjing University of Traditional Chinese Medicine's pharmacological evaluation room；96 orifice plates are public purchased from Costar
Department；RMPI1640 culture mediums are purchased from Gibco companies；DMEM culture mediums are purchased from Gibco companies；Newborn bovine serum is purchased from the Hangzhou four seasons
Green company；Tetrazolium bromide (MTT) and DMSO are purchased from Amresco companies；
Laboratory apparatus is as follows used in following embodiment：
Laboratory apparatus：Superclean bench (Suzhou purifies model SW-CJ-IFD), CO2Incubator (SANYO models：XD-
101), microplate reader BIO-RAD (Model NO.550Serial NO.16971).
Experimental method is as follows used in following embodiment：
Experimental method：The Traditional Chinese medicine compound composition that the embodiment of the present invention 1 is prepared is carried out using improvement mtt assay external
Antitumor cell is tested：Human tongue cancer cell line (Tca-8113) with pancreatin is digested, counts, be made a concentration of 5 × 104
The cell suspension of a/ml.100 μ l cell suspensions will be added in 96 orifice plates per hole (per hole 5 × 103A cell), it is subsequently placed in 37
DEG C, 5%CO2It is cultivated 24 hours in incubator；Drug is diluted to required different gradient concentrations with non-fully culture medium, is added per hole
Enter the 100 corresponding pastille culture mediums of μ L, while set up negative control group, vehicle control group and positive controls, then by 96 orifice plates
37 DEG C are placed in, 5%CO2It is cultivated 72 hours in incubator.Then 20 μ L MTT (5mg/ml) are added in per hole, continue culture 4 hours,
Culture is terminated, discards culture medium, adds in 150 μ L DMSO dissolvings per hole, the gently mixing of shaking table 10 minutes.λ=4570nm,
Absorbance, that is, OD values per hole are detected with microplate reader under two wavelength of 620nm, using the average value of each multiple holes as the OD of this group of cell
Value calculates the inhibiting rate of each drug.
Experimental result shows the Traditional Chinese medicine compound composition that the embodiment of the present invention 1 is prepared, and has to Human tongue cancer cell line
There is stronger inhibiting effect, each dosage group presents preferable dose-effect relationship.Experimental result is as shown in table 5.
Table 5 is to the exercising result of Human tongue cancer cell line
Implementation column 5 has experiment of the Traditional Chinese medicine compound composition of antitumor activity to immunoregulation effect
(1) to the influence of mouse IgA, IgG, IgM content
Experimental method：Take ICR small white mouses 30,18~22g of weight, be randomly divided into Normal group, lotus knurl control group and
Treatment group, traditional vaccination S180 sarcomas, 24 hours after inoculation, treatment group, which gavages the present invention, has the Chinese medicine compound prescription of antitumor activity
Composition (lot number 001) 0.3ml/ only (2g/kg), take a blood sample within the 15th day by small white mouse eyeball.IgA, IgG, IgM content are measured, specifically
Experimental result is shown in Table 6.
Table 6 is to the influence (mg/dl of mouse IgA, IgG, IgM content)
*P<0.01 (compared with Normal group) * * P<0.05 (compared with lotus knurl control group)
By with upper table 6 the experimental results showed that：Tumor-bearing mice Immunoglobulin IgA, IgG, IgM content are significantly lower than normal
Control mice；The present invention have antitumor activity Traditional Chinese medicine compound composition can improve the low IgA of tumor-bearing mice,
IgG, IgM content show that the Traditional Chinese medicine compound composition provided by the invention with antitumor activity, can with immunologic enhancement
To improve the immune function of tumor patient itself, so as to be conducive to the improvement of Quality of Life of Tumor Patients and itself antitumor work
With.
(2) to the influence of cAMP contents in rat plasma
Experimental method：Wistar big white mouse 45,150~180g of weight are taken, male and female dual-purpose is randomly divided into normal control
Group, lotus knurl control group and treatment group, traditional vaccination S180 sarcomas, after 24 hours, treatment group, which gavages the present invention, has antitumor work
Property Traditional Chinese medicine compound composition (lot number 002), 2ml/ only (2g/kg), continuous 14 days, Normal group gavage wait capacity physiology salt
Water, the 15th day, Rat Heart blood sampling measured cAMP contents, the results are shown in Table 7.
Influence of the table 7 to rat plasma cAMP contents
*P<0.05 (compared with lotus knurl control group)
By table 7 the experimental results showed that：Traditional Chinese medicine compound composition provided by the invention with antitumor activity, can make lotus
The low cAMP contents of knurl mouse improve (* P<0.05) Chinese medicine provided by the invention with antitumor activity, is further demonstrated that
Compound has the function of to significantly improve tumor patient immune function, is beneficial to the rehabilitation of tumor disease.
(3) raising that tumor necrosis factor (INF) induces is acted on
Experimental method：Wistar big white mouse 20 are taken, are randomly divided into control group and treatment group, every animal is injected intraperitoneally
10mg BCG vaccines, the 80 μ gLPS of intraperitoneal injection in the 15th day, take a blood sample after 90 minutes.Treatment group, which gavages the present invention, has antitumor work
Property Traditional Chinese medicine compound composition 2ml/ only (lot number 001,2g/kg), continuous 14 days, control group, which gavages, waited capacity physiological saline.
Test serum is taken, is diluted to various concentration；10% calf serums of target cell L929, RPM1-1640 culture solution tune
Whole is 2 × 106/ml cells, is added in 96 porocyte culture plates；100 μ l/ holes, then add the test serum 100 of different extension rates
μ l/ holes；37 DEG C are put, 5%CO2It is cultivated 18 hours in incubator.Result judgement：With the maximum dilution multiple of 50% target cell death
For INF potency.Concrete outcome is shown in Table 8.
The facilitation that table 8 induces rat INF
*P<0.001 (compared with Normal group)
By table 8 the experimental results showed that：Traditional Chinese medicine compound composition provided by the invention with antitumor activity can be apparent
Promote inducing for INF, (P<0.001), illustrate that the Traditional Chinese medicine compound composition provided by the invention with antitumor activity can pass through
Induced tumors necrosin INF is antitumor to achieve the purpose that.
The experimental results showed that, the Traditional Chinese medicine compound composition provided by the invention with antitumor activity is to tumour trouble by above
The immune function of person has raising effect, and cAMP contents and the generation for promoting tumor necrosis factor (INF) in adjustable body, can
To enhance patient itself anti-tumor function.
And the Traditional Chinese medicine compound composition clinical test results provided by the invention with antitumor activity are shown：To mammary gland
Cancer chemotherapy patient 60 and Chemotherapy of Cervical Cancer patient 50, take the Chinese medicine provided by the present invention with antitumor activity and answer
Square composition (embodiment of the present invention 1), through clinical observation in 2 months, takes the tumour of Traditional Chinese medicine compound composition provided by the invention
Patient, clinical symptoms, which have, to be obviously improved, and Life quality of lung cancer patients is improved, and toxicity significantly subtracts caused by chemotherapy
Gently, show the Traditional Chinese medicine compound composition provided by the present invention with antitumor activity to immune function with protection and improve
Effect, and human peripheral blood is as with protective effect, clinic observation effective percentage is respectively 83.5% and 80.7%.
The present invention carries out proportioning prescription using the theory of diagnosis and treatment, passes through more than experimental result according to traditional Chinese medical theory
Show the Traditional Chinese medicine compound composition provided by the invention with antitumor activity with anticancer removing toxic substances, dissipating phlegm and removing blood stasis, supplementing qi and nourishing yin
Effect has the growth and transferance for inhibiting tumour, and the drug resistance of energy reversing tumor cell, promotes withering for tumour cell
It dies, and without obvious toxic-side effects, illustrates that the Traditional Chinese medicine compound composition provided by the invention with antitumor activity can be used for making
Standby antineoplastic new drug.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of Traditional Chinese medicine compound composition with antitumor activity, which is characterized in that it by following parts by weight bulk pharmaceutical chemicals
It is made：12~36 parts of radix pseudostellariae, 10~30 parts of Radix Ophiopogonis, 20~60 parts of oldenlandia diffusa, 20~60 parts of Sculellaria barbata, edible tulip 15~
45 parts, 10~30 parts of curcuma zedoary；
Tumour is lung cancer, gastric cancer, colon cancer, cervical carcinoma, breast cancer or Dendritic cell.
2. the Traditional Chinese medicine compound composition according to claim 1 with antitumor activity, which is characterized in that it is by following heavy
The bulk pharmaceutical chemicals of amount number are made：12~24 parts of radix pseudostellariae, 10~20 parts of Radix Ophiopogonis, 20~40 parts of oldenlandia diffusa, Sculellaria barbata 20~
40 parts, 15~30 parts of edible tulip, 10~20 parts of curcuma zedoary.
3. the Traditional Chinese medicine compound composition according to claim 2 with antitumor activity, which is characterized in that it is by following heavy
The bulk pharmaceutical chemicals of amount number are made：12 parts of radix pseudostellariae, 10 parts of Radix Ophiopogonis, 20 parts of oldenlandia diffusa, 20 parts of Sculellaria barbata, 15 parts of edible tulip,
10 parts of curcuma zedoary.
4. the Traditional Chinese medicine compound composition according to any one of claims 1 to 3 with antitumor activity, which is characterized in that
The extract of each bulk pharmaceutical chemicals and pharmaceutically acceptable carrier are prepared into oral liquid, granule, capsule or Tabules
Drug.
5. claims 1 to 3 any one of them has the preparation method of the Traditional Chinese medicine compound composition of antitumor activity, feature
It is to include the following steps：
(1) curcuma zedoary is taken by claims 1 to 3 any one of them parts by weight, adds in the water of 8~16 times of amounts of curcuma zedoary medicinal material, adopt
With extraction by steam distillation 1~3 hour, volatile oil is collected；
(2) radix pseudostellariae, Radix Ophiopogonis, oldenlandia diffusa, Sculellaria barbata and mountain are taken by claims 1 to 3 any one of them parts by weight
Arrowhead adds in the curcuma zedoary dregs of a decoction after step (1) steam distillation, adds in the ethyl alcohol of total 6~12 times of volumes of medicinal material weight, reflux
Or continuous circumfluence extraction 2~3 times, 1~3 hour every time, merge extracting solution, be concentrated under reduced pressure, must concentrate under 50~80 degrees Celsius
Large pore resin absorption column on object, is first eluted with water, and it is 20%~70% ethanol elution then to use volumetric concentration successively；It collects and merges
Volumetric concentration is 55% ethanol eluate, is concentrated to give extract；
(3) volatile oil that step (1) is taken to obtain, the extract and pharmaceutically acceptable carrier that step (2) obtains are prepared into mouth
Take the drug of liquid, granule, capsule or Tabules.
6. the preparation method of the Traditional Chinese medicine compound composition with antitumor activity described in claim 5, which is characterized in that step
(2) macroporous absorbent resin described in is D101 resins.
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| CN107233449A (en) * | 2017-07-28 | 2017-10-10 | 青海九康中药饮片有限公司 | A kind of Traditional Chinese medicine compound composition with antitumor activity and its preparation method and application |
| CN109331146A (en) * | 2018-11-23 | 2019-02-15 | 厦门市中医院 | A kind of herbal mixture for treating liver cancer |
| CN111759993B (en) * | 2020-08-25 | 2021-12-17 | 江西国光药业有限公司 | Traditional Chinese medicine composition for treating liver cancer and preparation method thereof |
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| CN102357202A (en) * | 2011-10-17 | 2012-02-22 | 南京中医药大学 | Traditional Chinese medicine cancer toxin prescription for treating liver cancer and application thereof in pharmacy |
| CN103768534A (en) * | 2014-02-17 | 2014-05-07 | 南京中医药大学 | Traditional Chinese medicinal composition with anti-tumor activity |
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| CN103768534A (en) * | 2014-02-17 | 2014-05-07 | 南京中医药大学 | Traditional Chinese medicinal composition with anti-tumor activity |
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| 周仲瑛教授‘癌毒’学术思想探析;程海波等;《中华中医药杂志》;20100630;第25卷(第6期);第868页2.辨证用药原则段 * |
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