CN104758977B - A kind of alginate dressing for treating bedsore and preparation method thereof - Google Patents
A kind of alginate dressing for treating bedsore and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of alginate dressing for treating bedsore and preparation method thereof, the dressing has two-layer composite, i.e., is made up of bottom and surface layer, and bottom is alginate composite film, and surface layer is natural rubber film layer；Its preparation method, comprise the following steps：A, shark collagen solution is prepared；B, coaxial electrostatic spinning outer layer solution is prepared；C, coaxial electrostatic spinning sandwich layer solution is prepared；D, spinning；E, it is crosslinked；F, impregnate；G, epoxy natural rubber film is prepared；H, it is compound.Dressing prepared by the present invention has good biocompatibility, there are controllable growth conciliation, good hygroscopicity and anti-inflammatory, the antibiotic property of synthesising biological material again, the local supply of blood flow of wound, promotion organization growth can be improved, accelerate wound healing, good multifunctionality is shown, while there is good porosity, it is soft comfortable, compliance is good, it is easy to use, it is adapted in modern biomedical field, is particularly used with fields such as nursing treating patients with decubitus.
Description
Technical field
The invention belongs to medical product technical field, more particularly it relates to a kind of alginate for treating bedsore
Medical dressing, further relate to the preparation method of the dressing.
Background technology
Bedsore is also known as pressure ulcer or pressure sore, is one of common complication of internal medicine, is due to nerve and blood circulation
Obstacle, local organization continuous ischemia, malnutrition and occur soft tissue necrosis, it is that body is common by internal cause and external cause
Complicated pathologic process caused by effect, it is a kind of very refractory disease, once occurring, course for the treatment of length, easily recurrence, can causes from table
Skin brings great pain to the destruction of hypodermis, muscle, or even B＆J to patient, severe patient can scabies secondary infection, draw
Play septicemia and cause death.
In the U.S., there is bedsore complication in 4.3% inpatient, and its annual medical expense is up to multi-million dollar,
Britain also spends several hundred million pounds to be used to treat patients with bedsore every year, and China there is no complete statistics in this respect, but because of China
Populous, bed patient is more, and the bedsore incidence of disease is also constantly in high numerical value.For treating patients with decubitus, all do both at home and abroad a large amount of
Research work, but so far, still not finding the medicine and method of very effective treatment and nursing.Due to following
Cause in a series of Diseases, including after I, II, III and IV phase diabetic, brain damage stupor, hemiplegia, hemiplegia or
Paralytic patient, fracture-dislocation of cervical spine merge paralytic patient, thoracic spine fracture and dislocation merges paraplegia, burst fracture of lumbar merges paraplegia and suffered from
Person, osteoporosis cause patient that longer-term is liied in bed in fracture patient, late tumor patient and other affected treatments etc., have big
There is concurrent bedsore situation in amount, and therefore, the prophylactic treatment of bedsore and nursing turn into an arduousness and important work, Ye Shihu
The a great problem that science and engineering exists and be not fully solved in making for a long time.
At present, the conventional method of clinical treatment bedsore includes therapeutic method of surgery, drug treatment and outer coating method.
Operative treatment is mainly using skin flaps reparation after thorough debridement, but this method is not appropriate for for many patients；Medicine is treated
Method, including medicine for oral administration and externally applied drug, the shortcomings that also generally existing effect is not obvious enough.
Because outer coating method is simple to operation, and the injury to patient is minimum, and therefore, many clinical researches are also given greatly
Concern.Traditional wound dressing include cotton yarn bandage, bandage/package bandage, specialized dressing, dipping cotton yarn, slit close bar alternate product with
And emergency processing dressing sundry goods etc., major embodiment stop blooding, are fungi-proofing, preventing wound adhesion and simply wrap up function.Obviously, it is traditional
Wound dressing can not meet to require in treating patients with decubitus.
Therefore, the exploitation of bedsore specialized dressing and treatment method is also focused in some research work, such as：Patent publication No.
CN1593675A proposes a kind of bedsore dressing new method, is to use 2% acetic acid wash bedsore wound, then with the covering of Triterpene acid gauze and
Aseptic dressing is wrapped up, and obtains therapeutic effect；Patent publication No. CN101461960A proposes a kind of bedsore biological dressing, its mainly into
It is divided into chitosan, polyhexamethylene guanide, alum, two blooms, sesame oil, Gan Bo etc. to be mixed, the anti-inflammatory for various wounds stops
Blood, antibiotic and sterilizing, it is dry and comfortable prevent adhesion, promote skin histology heal and repair；Patent CN publication numbers 102885859A proposes a kind of
The medicine of bedsore is treated, pasty state is mixed into by Dragon bone(fried) powder, Yunnan Baiyao powder, 75% alcohol, first cleaned in treatment with hydrogen peroxide
Bedsore sore face, after irradiated with far IR curing instrument, then medicine is applied on sore face and plays therapeutic action；Patent CN publication numbers
103271794A propose it is a kind of based on bedsore wound biological antibiotic application, the application include chitosan, acetic acid, lysozyme,
The compositions such as gelatin, chlorhexidine acetate, aerosil and water, by ventilative base material, non-woven synthetic's silk, biological antibiotic layer, paper
Matter separation layer forms, for treating bedsore wound and burn and scald, Postoperative infection.
Research shows, keeps the moist environment of bedsore wound, controls the transmission situation of oxygen and water, forms closed micro-loop
Border, and possess can composite for born muscle antiphlogosis, promote cell growth condition, for treatment bedsore it is most important.Obviously, it is above-mentioned several
Item bedsore patent is most of simply simply using alginate, some Chinese and western drugses, the compound and simple therapy of simple material
Mode, design effectively new pattern compress is not considered how further, by considering the factor of physics and chemistry, biology,
On the one hand the moistening of compression position microenvironment is kept, controls surface of a wound effluent, stable wound temperature change；On the other hand, control
The transmitance of oxygen and vapor, the suitable partial pressure of oxygen in compression position is maintained, promotes microcirculation to be formed；Simultaneously in view of antibacterial with preventing
Only infect, construct the barrier of one layer of defense against bacterial intrusion in skin surface, strengthen the anti-inflammatory power of tissue；Then, further
Consider to utilize with promotion wound healing, the growth of fibroblast and endothelial cell can be stimulated and promote keratinocyte proliferation
Composition, reach strengthen wound healing with lifted therapeutic effect purpose.
At present, China market has that the high-end dressing for being used to treat bedsore of independent intellectual property right is also seldom, and can be comprehensive
Conjunction is considered to maintain bedsore wound moistening and oxygen, moisture microenvironment, considers using the anti-infection of high-efficiency antimicrobial and further to apply
Modern bedsore dressing, almost blank are prepared to the material that can speed up wound healing.Therefore, new high-end bedsore is studied
Dressing technology of preparing and preparation method, are extremely important.
The content of the invention
It is an object of the invention to provide alginate dressing of a treatment bedsore and preparation method thereof, its therapeutic effect is good,
It is convenient to prepare, and is easy to promote.
To achieve the above object, the technical scheme taken of the present invention is：
A kind of alginate medical dressing for treating bedsore, the dressing has two-layer composite, i.e., by bottom and surface layer
Form, bottom is alginate composite film, and surface layer is natural rubber film layer.
, should 1. the natural rubber film layer is the epoxy natural rubber material film that epoxidation level reaches 30~40%
The thickness of film is 0.04~0.1 millimeter；The alginate composite film is by sodium alginate, Sodium Polyacrylate, shark collagen
The fiber that albumen, pigskin collagen, chitosan quaternary ammonium salt, basic fibroblast growth factor (bFGF) different materials are formed
Film, the thickness of the film is 1~5 millimeter；The tunica fibrosa is to be arranged under the overlay to form by filament, and the filament has core, shell knot
Structure, wherein, shell is made up of sodium alginate, Sodium Polyacrylate, shark collagen, pigskin collagen material；Sandwich layer is by shell
Glycan quaternary ammonium salt and growth factor material are formed, and tunica fibrosa surface is coated with the material layer of chloride containing calcium and zinc chloride and by big gun
The bletilla of system, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle, Asian puccoon extract material layer.
A kind of preparation method for the alginate medical dressing for treating bedsore, comprises the following steps：
A, shark collagen solution is prepared
The mixture dry product of sharkskin and bone is taken, a certain amount of NaOH solution immersion is added after crushing, is drained, adds petroleum ether
After immersion, drain, after adding acetic acid solution extraction, centrifugation, take supernatant to add metabisulfite solution to precipitate, sediment is through distilled water
Washing, freeze-drying, obtain sharkskin and bone mixing collagen, are dissolved in certain density acetic acid solution, obtain shark
Isin glue collagen solution.
B, coaxial electrostatic spinning outer layer solution is prepared
The solution for taking step A to obtain, sodium alginate, Sodium Polyacrylate, pigskin collagen are added, is uniformly mixed,
Obtain a kind of composite solution containing alginate mixture；
C, coaxial electrostatic spinning sandwich layer solution is prepared
Chitosan quaternary ammonium salt is dissolved in distilled water, adds basic fibroblast growth factor (bFGF), stirring is equal
It is even, obtain a kind of composite solution containing chitosan quaternary ammonium salt and growth factor；
D, spinning
Using coaxial electrostatic spinning method, the alginate composite solution that step B is obtained is allowed as the outer of coaxial electrostatic spinning
Layer solution, allows the composite solution for containing chitosan quaternary ammonium salt and growth factor that step C is obtained as in coaxial electrostatic spinning
Layer solution, carries out coaxial spinning, obtains filament with nucleocapsid double-decker based on alginate and by filament
The tunica fibrosa of composition；
E, it is crosslinked
The tunica fibrosa that step D is obtained immerses some time in chloride mixture solution, in hot blast condition after taking-up
The lower dry some time, obtain cross-linked alginate compound；
F, impregnate
The cross-linked alginate compound that step E is obtained is immersed containing the bletilla striata, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle, Asian puccoon composition
The some time is placed in compound Chinese medicine water cooking liquid, takes out and certain time is dried under the conditions of hot blast, obtain the friendship containing traditional Chinese medicine ingredients
Join alginate compound；
G, epoxy natural rubber film is prepared
The tape casting is used to prepare thickness as 0.04~0.1 millimeter of epoxy natural rubber film；
H, it is compound
The epoxy natural rubber Film laminated that the compound that step F is obtained obtains with step G, that is, obtain described control
Treat the alginate dressing of bedsore.
It is preferred that step A in the mixture dry product of sharkskin and bone be sharkskin and Shark cartilage ratio be respectively 6~
7:4~3 mixtures, 5% dry product is less than by drying to water content；It is addition 1 after fineness 20-50 mesh that the dry product, which crushes,:15
The 0.1N/L NaOH solutions of quality, soak 5 hours at a temperature of 8 DEG C, taking-up drains；Add 1:10 petroleum ether, at a temperature of 8 DEG C
Immersion 12 hours, with distillation water washing after drain；Again plus 1:10 2.5%-3.5% acetic acid solution and at 8 DEG C, stirring speed
Spend and stirred 48 hours for 300~500rpm；Centrifuged by 40000 turns/min, take supernatant to add 1mol/L sodium sulphate, by heavy
Form sediment, filter, distillation water washing, then adding 1:10 2.5%-3.5% acetic acid solution dissolving, uses molecular cut off as 100kDa
Milipore filter ultrafiltration, filtrate freeze-drying, obtains sharkskin and cartilage mixing collagen, then takes 2% acetic acid solution to dissolve, and is made into
The protein concentration of original containing epoxy glue is 0.3% solution
It is preferred that step B described in sodium alginate addition be 0.4~0.8%, sodium alginate and Sodium Polyacrylate
Weight ratio is 1:0.2~0.4, the weight ratio of sodium alginate and pigskin collagen is 1:0.05~0.08, the pigskin collagen is
For molecular weight in 10D to 30D low molecule collagen, mixing speed is 300~500rpm, is stirred 20 minutes.
It is preferred that step C described in chitosan quaternary ammonium salt and growth factor composite solution in chitosan quaternary ammonium salt be shell
The quaternised modified product of glycan, content are 0.3~0.5%；Basic fibroblast growth factor (bFGF) content is
0.001%, mixing speed is 300~500rpm, is stirred 10 minutes.
It is preferred that step D described in coaxial electrostatic spinning method, be with the alginate composite solution obtained by step B
As the outer layer solution of spinning, resulting chitosan-containing quaternary ammonium salt and growth factor composite solution are used as spinning using in step C
Internal layer solution, voltage be 30kV, the distance between fixed anode and negative electrode be 18cm, temperature is 28~30 DEG C of condition
Under, coaxial electrostatic spinning is carried out, filament faint yellow, with core shell structure is obtained on receiver board and is made up of filament
Alginate fiber film.
It is preferred that step E described in chloride mix solution be the mixing water containing 3% calcium chloride and 0.5% zinc chloride
Solution, after immersion standing time be 20 to 30 minutes, then take out, in 35~40 DEG C of hot-airs dry, drying time be 1~
2 hours.
It is preferred that step F described in compound Chinese medicine water cooking liquid, be bletilla, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle, Asian puccoon each 10
Gram, 120 grams of water are added, after soaking 20 minutes, boils 25 minutes, pours out liquid, the dregs of a decoction add water 100 grams, boil 20 points again
Clock, merge liquid twice, add 200 milliliters of water constant volume, turn into compound Chinese medicine water cooking liquid；Cross-linked alginate compound immerses Chinese medicine
Standing time is 2 minutes after water cooking liquid, then takes out drying, is dried in 35~40 DEG C of hot-airs, and drying time is 1~2 small
When.
It is preferred that step G described in epoxy natural rubber film be epoxidation day that epoxidation level is 30~40%
T PNR, it is dissolved in tetrahydrofuran, is configured to 1% solution, cleaning is then coated on not in sticky paper, by 68 by the tape casting
~70 DEG C of hot-air is dried, and drying time is 40 minutes, and the thickness of acquisition is 0.04~0.1 millimeter of epoxy natural rubber
Film.
Sharkskin and Shark cartilage used in the present invention are current market sales of products, such as attain Mei Da by Beihai
The product that marine organisms Products Co., Ltd is sold with trade name shark cartilage powder and sharkskin powder.
The milipore filter that the present invention uses is current market sales of product, such as is supplied by Beijing Tao Shi milipore filters company
With trade name SFP2680 milipore filters or Guangzhou Chao Yu membrane separation techniques Co., Ltd with trade name CYG90S polysulphone super-filter membrane pins
The product sold.
If the Shark cartilage content that uses of the present invention is less than 30%, can make the anti-oxidant of dressing, Green Tea Extract and
Antiinflammation reduces；If the Shark cartilage content that the present invention uses is higher than 40%, Anti-angiogenesis factors content occurs
It is too high, unfavorable wound healing.Therefore, the Shark cartilage content that uses of the present invention is suitable preferably 32 for 30~40%
~38%, more preferably 34~36%.
It is current market sales of product that the sodium alginate that the present invention uses, which is, such as is had by Qingdao Huanghai Sea bio-pharmaceuticals
The product or seaweed industry Co., Ltd is extra large with trade name everyday by Lianyun Harbour that limit responsible company is sold with trade name sodium alginate
The product of mosanom sale.
The Sodium Polyacrylate that the present invention uses is current market sales of product, such as limited by Shandong KeYu water process
What company was sold with trade name Sodium Polyacrylate or by Jiaozuo City Tai Feng Fine Chemical Co., Ltd with trade name Sodium Polyacrylate
Product.
The pigskin collagen that the present invention uses is by biomedical engineering research institute of Ji'nan University patented technology
(CN1470640) pigskin collagen prepared.
If the sodium alginate content that the present invention uses is less than 0.4%, follow-up electrostatic spinning can be made not carry out；
If the sodium alginate content that the present invention uses is higher than 0.8%, occur that dissolving is not easy problem.Therefore, the present invention makes
Sodium alginate content be 0.4~0.8% be it is suitable, preferably 0.5~0.8%, more preferably 0.6~0.7%.
If the sodium polyacrylate content that the present invention uses is less than 0.2, the water imbibition for the dressing that can be prepared is not by
Profit influences；If the sodium polyacrylate content that the present invention uses is higher than 0.4, the dissolving of follow-up collagen can be made by shadow
Ring.Therefore, the sodium polyacrylate content that uses of the present invention is suitable preferably 0.3~0.4% for 0.2~0.4, more excellent
Selection of land is 0.4%.
If the pigskin collagen content that uses of the present invention is less than 0.05, nursing skin and wound can not be played
Effect；If the collagen content that uses of the present invention is higher than 0.08, the problem of influenceing electrostatic spinning occurs.Therefore,
The collagen content that the present invention uses be 0.05~0.08 be it is suitable, preferably 0.06~0.08, more preferably
0.07~0.08.
The chitosan quaternary ammonium salt that the present invention uses is current market sales of product, such as by the green refreshing bioengineering in Nantong
The product that Co., Ltd is sold with trade name chitosan quaternary ammonium salt.
The bFGF growth factors that the present invention uses are by Bio-engineering Institute of Jinan University's patented technology (CN1500809)
The bFGF growth factors of preparation.
If the chitosan quaternary ammonium salt content that the present invention uses is less than 0.3%, dressing can be made not embodied anti-
Bacterium antiinflammation；If the chitosan quaternary ammonium salt content that the present invention uses is higher than 0.5%, has influence on growth factor and play just
Chang Xiaoneng.Therefore, the chitosan quaternary ammonium salt content that uses of the present invention for 0.3~0.5% is suitable, preferably 0.4~
0.5%.
It is current market sales of product that the calcium chloride that the present invention uses, which is, such as limited by Shandong Eastcom chemical industry
Product that company is sold with trade name calcium chloride or the production sold by Weifang City Divine Land Chemical Co., Ltd. with trade name calcium chloride
Product.
The zinc chloride that the present invention uses is current market sales of product, such as is had by Hebei province Jinzhou City Ji Tian Xinye
Limit company is with trade name zinc chloride or the product sold by Guangzhou Reagent Company with trade name zinc chloride.
If the time that tunica fibrosa is immersed in mixed liquor in the present invention is less than 20 minutes, tunica fibrosa can be made because being crosslinked not
Influence the performance of dressing enough；If tunica fibrosa immerse mixed liquor in time be higher than 30 minutes, can because be crosslinked excessively and
Reduce the imbibition effect of dressing.Therefore, immersion standing time for using of the present invention is 20 to 30 minute, most preferably 25 minutes.
The Chinese herbal medicine bletilla striata that the present invention uses, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle, Asian puccoon are current market sales of products, example
The product such as sold by Guangzhou north and south row prepared slices of Chinese crude drugs Co., Ltd with the trade name bletilla striata, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle, Asian puccoon.
The epoxy natural rubber that the present invention uses is by Product processing research institute of Chinese Academy of Tropical Agricultural Sciences patent skill
The epoxy natural rubber of art (CN101654488) production.
If the epoxidation level for the epoxy natural rubber that the present invention uses is less than 30%, the close of dressing can be influenceed
Close effect；If the epoxidation level for the epoxy natural rubber that the present invention uses is higher than 40%, film breathable occurs
Property is too low, and vapor is not easy to distribute, and influences facilitation of the dressing to wound healing.Therefore, the epoxidation that the present invention uses
The epoxidation level of natural rubber be 30~40% be it is suitable, preferably 31~37%, more preferably 32~35%.
In above step, in hot-air drying equipment dry article reach water content less than by weight 5% with
Under.Centrifugation apparatus, the drying equipment used in above step be in the art it is usually used, sold in the market
The product sold.The water content of the article determines to obtain using Weighing method is dried.The electrostatic spinning that the present invention uses is set
Standby is the rotary drum electrostatic spinning using the manufacture of biomedical engineering research institute of Ji'nan University patent (CN103060932A) technology
Machine.
The beneficial effects of the invention are as follows：
1. using part of the shark collagen as dressing, the good anti-oxidant, anti-of shark collagen is both utilized
Free radical and anti-inflammatory with promote bedsore wound Healing, use again the angiogenesis inhibitor of Shark cartilage collagen and polysaccharide because
Sub- active component, the control surface of a wound will not produce hyperblastosis in agglutination, be formed without obvious scar.
2. using sodium alginate and main part of the Sodium Polyacrylate as dressing, the good imbibition of alginate is both utilized
Performance, again using the high-hygroscopicity of Sodium Polyacrylate, ensures that the surface of a wound is constantly in moist environment, had with promoting skin growth
Beneficial to the healing of the surface of a wound.Meanwhile pigskin low molecule collagen is also further utilized, play the work for promoting surface of a wound health healing
With.
3. utilizing coaxial spining technology, using chitosan quaternary ammonium salt and growth factor as inner nuclear layer, slow releasing function is produced, both
Anti-inflammation is played, and can enough promotes skin to grow；Using electrostatic spinning technique, by alginate and shark collagen, low point
The spinned fiber of sub- collagen, fibre structure is controlled, adjust gas permeability, and form good microbial barrier, the performance of dust.
In addition, the nanofiber prepared has multifunctionality, possesses up to 100m2/ more than g specific surface area, there is good imbibition to inhale
It is moist, the effluent of wound can be effectively absorbed, the biotic environment of the surface of a wound can be ensured, is advantageous to the healing of the surface of a wound, mitigates disease
The pain of people.
4. being combined using zinc with calcium divalent ion, as crosslinking agent, part sodium alginate is set to pass through ion exchange, generation two
Valency ionomer complex compound, enable dressing that there is good physical and mechanical properties and water indissolubility, meanwhile, covered using dressing
The surface of a wound produces ion exchange, discharges zinc ion, zinc ion permeates in the surface of a wound, accelerates epidermal cell movement, and improve with contacting
The power of regeneration of cell, wound healing.
5. applied using the compound Chinese medicine water cooking liquid dipping containing the Chinese herbal medicine bletilla striata, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle, Asian puccoon composition
Material, utilize the good astringing to arrest bleeding of these Chinese medicines, scattered stasis detumescence, blood activating and promoting tissue regeneration, the removing toxic substances for the treatment of sore and wound healing growth
Effect, further improve healing speed.
6. research shows, the intrusion of extraneous bacterium can be effectively completely cut off using seal dressing, infection rate is greatly reduced, and
Prevent infective wound surface spread of germs and cause nosocomialtion, meanwhile, containing rich in the wound fluid that closed environment stores
Rich macrophage, lymphocyte, monocyte etc., the host phagocytes for being advantageous to leucocyte mediation play a role, and strengthen
Local bactericidal and tissue regeneration ability.The present invention utilizes the good air-tightness of epoxidezed rubber, and there is conciliation oxygen to be steamed with water
The effect of impervious speed, the epoxy natural rubber film that thickness is 0.04~0.1 millimeter is used to maintain to create as outermost layer
The relatively closed and moist environment in face, strengthen leukocyte function, accelerate epithelialization, granulation formation, cellulose and the necrosis of the surface of a wound
The degraded of material, suppress the breeding and diffusion of bacterium, so as to greatly shorten the time of wound healing.
Dressing prepared by the present invention is a kind of compound, has machinery, physics and chemistry, the biology of modern dressing
Can, the good biocompatibility of existing natural biologic material, there is the controllable growth of synthesising biological material to reconcile again, good hygroscopicity
With anti-inflammatory, antibiotic property, the local supply of blood flow of wound can be improved, promotion organization growth, accelerate wound healing, shown good
Multifunctionality, while there is good porosity, soft comfortable, compliance is good, easy to use, is adapted in modern biotechnology
Medical domain, particularly used with fields such as nursing treating patients with decubitus, the alginate dressing for preparing of the present invention can be applied to wrap
Stupor, hemiplegia, hemiplegia or paralytic patient, fracture of cervical spine is caused to take off after including I, II, III and IV phase diabetic, brain damage
Position merges paralytic patient, thoracic spine fracture and dislocation merges paraplegia, burst fracture of lumbar merges paralytic patient, osteoporosis causes fracture
Treatment and nursing of the concurrent bedsore such as patient that longer-term is liied in bed in patient, late tumor patient and other affected treatments etc. are used
On the way.
Brief description of the drawings
Fig. 1 is the structural representation of alginate medical dressing of the embodiment of the present invention.
Fig. 2 is the filametntary structural representation of the embodiment of the present invention.
Embodiment
Embodiment 1
2. as shown in Figure 1 and Figure 2, a kind of alginate medical dressing for treating bedsore, the dressing have two-layer compound knot
Structure, i.e., it is made up of bottom and surface layer, bottom is alginate composite film 2, and surface layer is natural rubber film layer 1.Natural rubber film
Layer 1 is the epoxy natural rubber material film that epoxidation level reaches 30~40%, and the thickness of the film is 0.04~0.1 milli
Rice；Alginate composite film 2 is by sodium alginate, Sodium Polyacrylate, shark collagen, pigskin collagen, chitosan
The tunica fibrosa that quaternary ammonium salt, basic fibroblast growth factor (bFGF) different materials are formed, the thickness of the film is 1~5 millimeter；
Tunica fibrosa is to be arranged under the overlay to form by filament, and the filament has core 4, the structure of shell 3, and wherein shell is by sodium alginate, poly- third
Olefin(e) acid sodium, shark collagen, pigskin collagen material are formed；Sandwich layer is by chitosan quaternary ammonium salt and growth factor material structure
Into material layer of the tunica fibrosa surface coated with chloride containing calcium and zinc chloride and the bletilla by processing, the radix paeoniae rubrathe, dragon's blood, soil
Momordica cochinchiensis, Asian puccoon extract material layer.
A kind of preparation method for the alginate medical dressing for treating bedsore, comprises the following steps：
A, the content ratio for taking sharkskin that smashing fineness is 20 mesh and Shark cartilage is 6.7:3.4 mixture dry product,
Add 1:The 0.1N/L NaOH solutions of 15 mass, soak 5 hours at a temperature of 8 DEG C, taking-up drains；Add 1:10 petroleum ether, 8
Soaked 12 hours at a temperature of DEG C, with distillation water washing after drain；Again plus 1:10 2.5% acetic acid solution, at 8 DEG C, stirring speed
Stir 48 hours under conditions of degree 300rpm, centrifuged under conditions of 40000 turns/min；Supernatant is taken to add 1mol/L sodium sulphate,
By precipitating, filtering, distilling water washing, then add 1:10 2.5% acetic acid solution dissolving, using Beijing Tao Shi milipore filters company
The molecular cut off of supply is 100kDa milipore filter ultrafiltration, filtrate freeze-drying, obtains sharkskin and cartilage mixing collagen egg
In vain, then the dissolving of 2% acetic acid solution is taken to be made into the solution that the protein concentration of original containing epoxy glue is 0.3%；
B, the solution for taking step A to prepare, under conditions of low whipping speed 300rpm, sodium alginate 0.4% is added；Add poly-
The weight ratio of PAA, sodium alginate and Sodium Polyacrylate is 1:0.2；Add pigskin collagen, sodium alginate and pigskin
The weight ratio of collagen is 1:0.05, stir 20 minutes；
C, using distilled water as solvent, basic fibroblast growth factor (bFGF), low whipping speed 300rpm bar are taken
Under part, chitosan quaternary ammonium salt and growth factor composite solution are prepared, chitosan quaternary ammonium salt content is given birth to for 0.3%, bFGF in solution
Long factor content is 0.001%, is stirred 10 minutes；
D, using in step B obtained by alginate composite solution be used as the outer layer solution of spinning, with resulting in step C
Internal layer solution as spinning of chitosan-containing quaternary ammonium salt and growth factor composite solution, coaxial electrostatic spinning is carried out, in voltage
It is 18cm for the distance between 30kV, fixed anode and negative electrode, under conditions of temperature is 28~30 DEG C, carries out coaxial electrostatic spinning
Silk, obtains filament faint yellow, with core shell structure and the alginate fiber film being made up of filament on receiver board；
E, using distilled water as solvent, using calcium chloride and zinc chloride, the chlorine containing 3% calcium chloride and 0.5% zinc chloride is prepared
Compound mixed liquor, the nano fibrous membrane obtained by D steps is immersed in mixed liquor, then takes out drying.It is 20 to immerse standing time
Minute, dried in 35 DEG C of hot-airs, drying time is 2 hours；
F, each 10 grams of the bletilla striata, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle, Asian puccoon Chinese medicine are taken, adds 120 grams of water, after soaking 20 minutes,
Boil 25 minutes, pour out liquid, the dregs of a decoction add water 100 grams, boil again 20 minutes, merge liquid twice, add the milli of water constant volume 200
Rise, turn into compound Chinese medicine water cooking liquid；Cross-linked alginate compound made from E steps is taken to immerse compound Chinese medicine water cooking liquid, then
Drying is taken out, it is 2 minutes to immerse herbs ' water-decoctum standing time, and 35 DEG C of hot-airs are dried, and drying time is 2 hours；
G, the epoxy natural rubber for taking epoxidation level to be 32%, is dissolved in tetrahydrofuran, is configured to 1% solution,
Then cleaning is coated on not in sticky paper by the tape casting, by 68 DEG C of hot-airs drying, drying time is 2 hours, acquisition
Thickness is 0.04 millimeter of epoxy natural rubber film；
H, it is compound
The epoxy natural rubber Film laminated that the compound that step F is obtained obtains with step G, that is, obtain described control
Treat the alginate dressing of bedsore.
Embodiment 2
A kind of preparation method for the alginate medical dressing for treating bedsore, comprises the following steps：
A, the content ratio for taking sharkskin that smashing fineness is 50 mesh and Shark cartilage is 6.4:3.6 mixture dry product,
Add 1:The 0.1N/L NaOH solutions of 15 mass, soak 5 hours at a temperature of 8 DEG C, taking-up drains；Add 1:10 petroleum ether, 8
Soaked 12 hours at a temperature of DEG C, with distillation water washing after drain；Again plus 1:10 3.5% acetic acid solution, at 8 DEG C, stirring speed
Stir 48 hours under conditions of degree 500rpm, centrifuged under conditions of 40000 turns/min；Supernatant is taken to add 1mol/L sodium sulphate,
By precipitating, filtering, distilling water washing, then add 1:10 3.5% acetic acid solution dissolving, surpasses Yu's membrane separation technique using Guangzhou
The milipore filter ultrafiltration that the molecular cut off of Co., Ltd's supply is 100kDa, filtrate freeze-drying, obtains sharkskin and cartilage mixes
Collagen is closed, then takes the dissolving of 2% acetic acid solution to be made into the solution that the protein concentration of original containing epoxy glue is 0.3%.
B, the solution for taking step A to prepare, under conditions of low whipping speed 500rpm, sodium alginate 0.7% is added；Add poly-
The weight ratio of PAA, sodium alginate and Sodium Polyacrylate is 1:0.4；Add pigskin collagen, sodium alginate and pigskin
The weight ratio of collagen is 1:0.08, stir 20 minutes；
C, bFGF growth factors are taken using distilled water as solvent, under conditions of low whipping speed 500rpm, prepare chitosan season
Ammonium salt and growth factor composite solution, chitosan quaternary ammonium salt content is that 0.5%, bFGF growth factor contents are in solution
0.001%, stir 10 minutes；
D, using in step B obtained by alginate composite solution be used as the outer layer solution of spinning, with resulting in step C
Internal layer solution as spinning of chitosan-containing quaternary ammonium salt and growth factor composite solution, coaxial electrostatic spinning is carried out, in voltage
It is 18cm for the distance between 30kV, fixed anode and negative electrode, under conditions of temperature is 28~30 DEG C, carries out coaxial electrostatic spinning
Silk, obtains filament faint yellow, with core shell structure and the alginate fiber film being made up of filament on receiver board；
E, using distilled water as solvent, using calcium chloride and zinc chloride, the chlorine containing 3% calcium chloride and 0.5% zinc chloride is prepared
Compound mixed liquor, the nano fibrous membrane obtained by D steps is immersed in mixed liquor, then, take out drying, it is 30 to immerse standing time
Minute, 40 DEG C of hot-air is dried, and drying time is 1 hour；
F, each 10 grams of the bletilla striata, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle, Asian puccoon Chinese medicine are taken, adds 120 grams of water, after soaking 20 minutes,
Boil 25 minutes, pour out liquid, the dregs of a decoction add water 100 grams, boil again 20 minutes, merge liquid twice, add the milli of water constant volume 200
Rise, turn into compound Chinese medicine water cooking liquid；Cross-linked alginate compound made from E steps is taken to immerse compound Chinese medicine water cooking liquid, then
Take out drying, immerse herbs ' water-decoctum standing time be 2 minutes, hot blast condition be 40 DEG C of temperature hot-air, drying time 1
Hour；
G, the epoxy natural rubber for taking epoxidation level to be 35%, is dissolved in tetrahydrofuran, is configured to 1% solution,
Then cleaning is coated on not in sticky paper by the tape casting, 70 DEG C of hot-air is dried, and drying time is 1 hour, the thickness of acquisition
For 0.1 millimeter of epoxy natural rubber film.
H, it is compound
The epoxy natural rubber Film laminated that the compound that step F is obtained obtains with step G, that is, obtain described control
Treat the alginate dressing of bedsore.
Embodiment 3
A kind of preparation method for the alginate medical dressing for treating bedsore, comprises the following steps：
A, the content ratio for taking sharkskin that smashing fineness is 35 mesh and Shark cartilage is 6:4 mixture dry product, add
1:The 0.1N/L NaOH solutions of 15 mass, soak 5 hours at a temperature of 8 DEG C, taking-up drains；Add 1:10 petroleum ether, 8 DEG C of temperature
The lower immersion of degree 12 hours, with being drained after distillation water washing；Again plus 1:10 3.0% acetic acid solution, at 8 DEG C, mixing speed
Stir 48 hours under conditions of 400rpm, centrifuged under conditions of 40000 turns/min；Supernatant is taken to add 1mol/L sodium sulphate, warp
Precipitation, filtering, distillation water washing are crossed, then adds 1:10 3.0% acetic acid solution dissolving, surpassing Yu's membrane separation technique using Guangzhou has
The milipore filter ultrafiltration that the molecular cut off of limit company supply is 100kDa, filtrate freeze-drying, obtain sharkskin and cartilage mixing
Collagen, then take the dissolving of 2% acetic acid solution to be made into the solution that the protein concentration of original containing epoxy glue is 0.3%；
B, the solution for taking step A to prepare, under conditions of low whipping speed 400rpm, sodium alginate 0.8% is added；Add poly-
The weight ratio of PAA, sodium alginate and Sodium Polyacrylate is 1:0.3；Add pigskin collagen, sodium alginate and pigskin
The weight ratio of collagen is 1:0.06, stir 20 minutes；
C, bFGF growth factors are taken using distilled water as solvent, under conditions of low whipping speed 400rpm, prepare chitosan season
Ammonium salt and growth factor composite solution, chitosan quaternary ammonium salt content is that 0.4%, bFGF growth factor contents are in solution
0.001%, stir 10 minutes；
D, using in step B obtained by alginate composite solution be used as the outer layer solution of spinning, with resulting in step C
Internal layer solution as spinning of chitosan-containing quaternary ammonium salt and growth factor composite solution, coaxial electrostatic spinning is carried out, in voltage
It is 18cm for the distance between 30kV, fixed anode and negative electrode, under conditions of temperature is 28~30 DEG C, carries out coaxial electrostatic spinning
Silk, obtains filament faint yellow, with core shell structure and the alginate fiber film being made up of filament on receiver board；
E, using distilled water as solvent, using calcium chloride and zinc chloride, the chlorine containing 3% calcium chloride and 0.5% zinc chloride is prepared
Compound mixed liquor, the nano fibrous membrane obtained by D steps is immersed in mixed liquor, then, take out drying, it is 25 to immerse standing time
Minute, 40 DEG C of hot-air is dried, and drying time is 1.5 hours；
F, each 10 grams of the bletilla striata, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle, Asian puccoon Chinese medicine are taken, adds 120 grams of water, after soaking 20 minutes,
Boil 25 minutes, pour out liquid, the dregs of a decoction add water 100 grams, boil again 20 minutes, merge liquid twice, add the milli of water constant volume 200
Rise, turn into compound Chinese medicine water cooking liquid；Cross-linked alginate compound made from E steps is taken to immerse compound Chinese medicine water cooking liquid, then
Drying is taken out, it is 2 minutes to immerse herbs ' water-decoctum standing time, and hot blast condition is the hot-air of 37 DEG C of temperature, and drying time is
1.5 hour；
G, the epoxy natural rubber for taking epoxidation level to be 40%, is dissolved in tetrahydrofuran, is configured to 1% solution,
Then cleaning is coated on not in sticky paper by the tape casting, 69 DEG C of hot-air is dried, and drying time is 1 hour, the thickness of acquisition
For 0.08 millimeter of epoxy natural rubber film；
H, it is compound
The epoxy natural rubber Film laminated that the compound that step F is obtained obtains with step G, that is, obtain described control
Treat the alginate dressing of bedsore.
Embodiment 4
A kind of preparation method for the alginate medical dressing for treating bedsore, comprises the following steps：
A, the content ratio for taking sharkskin that smashing fineness is 40 mesh and Shark cartilage is 7:3 mixture dry product, add
1:The 0.1N/L NaOH solutions of 15 mass, soak 5 hours at a temperature of 8 DEG C, taking-up drains；Add 1:10 petroleum ether, 8 DEG C of temperature
The lower immersion of degree 12 hours, with being drained after distillation water washing；Again plus 1:10 2.8% acetic acid solution, at 8 DEG C, mixing speed
Stir 48 hours under conditions of 3500rpm, centrifuged under conditions of 40000 turns/min；Supernatant is taken to add 1mol/L sodium sulphate, warp
Precipitation, filtering, distillation water washing are crossed, then adds 1:10 2.8% acetic acid solution dissolving, surpassing Yu's membrane separation technique using Guangzhou has
The milipore filter ultrafiltration that the molecular cut off of limit company supply is 100kDa, filtrate freeze-drying, obtain sharkskin and cartilage mixing
Collagen, then take the dissolving of 2% acetic acid solution to be made into the solution that the protein concentration of original containing epoxy glue is 0.3%；
B, the solution for taking step A to prepare, under conditions of low whipping speed 350rpm, sodium alginate 0.6% is added；Add poly-
The weight ratio of PAA, sodium alginate and Sodium Polyacrylate is 1:0.25；Add pigskin collagen, sodium alginate and pigskin
The weight ratio of collagen is 1:0.07, stir 20 minutes；
C, bFGF growth factors are taken using distilled water as solvent, under conditions of low whipping speed 350rpm, prepare chitosan season
Ammonium salt and growth factor composite solution, chitosan quaternary ammonium salt content is that 0.35%, bFGF growth factor contents are in solution
0.001%, stir 10 minutes；
D, using in step B obtained by alginate composite solution be used as the outer layer solution of spinning, with resulting in step C
Internal layer solution as spinning of chitosan-containing quaternary ammonium salt and growth factor composite solution, coaxial electrostatic spinning is carried out, in voltage
It is 18cm for the distance between 30kV, fixed anode and negative electrode, under conditions of temperature is 28~30 DEG C, carries out coaxial electrostatic spinning
Silk, obtains filament faint yellow, with core shell structure and the alginate fiber film being made up of filament on receiver board；
E, using distilled water as solvent, using calcium chloride and zinc chloride, the chlorine containing 3% calcium chloride and 0.5% zinc chloride is prepared
Compound mixed liquor, the nano fibrous membrane obtained by D steps is immersed in mixed liquor, then, take out drying, it is 28 to immerse standing time
Minute, 36 DEG C of hot-air is dried, and drying time is 1.2 hours；
F, each 10 grams of the bletilla striata, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle, Asian puccoon Chinese medicine are taken, adds 120 grams of water, after soaking 20 minutes,
Boil 25 minutes, pour out liquid, the dregs of a decoction add water 100 grams, boil again 20 minutes, merge liquid twice, add the milli of water constant volume 200
Rise, turn into compound Chinese medicine water cooking liquid；Cross-linked alginate compound made from E steps is taken to immerse compound Chinese medicine water cooking liquid, then
Drying is taken out, it is 2 minutes to immerse herbs ' water-decoctum standing time, and hot blast condition is the hot-air of 36 DEG C of temperature, and drying time is
1.5 hour；
G, the epoxy natural rubber for taking epoxidation level to be 30%, is dissolved in tetrahydrofuran, is configured to 1% solution,
Then cleaning is coated on not in sticky paper by the tape casting, 68 DEG C of hot-air is dried, and drying time is 1 hour, the thickness of acquisition
For 0.06 millimeter of epoxy natural rubber film；
H, it is compound
The epoxy natural rubber Film laminated that the compound that step F is obtained obtains with step G, that is, obtain described control
Treat the alginate dressing of bedsore.
Embodiment 4
A kind of preparation method for the alginate medical dressing for treating bedsore, comprises the following steps：
A, the content ratio for taking sharkskin that smashing fineness is 40 mesh and Shark cartilage is 7:3 mixture dry product, add
1:The 0.1N/L NaOH solutions of 15 mass, soak 5 hours at a temperature of 8 DEG C, taking-up drains；Add 1:10 petroleum ether, 8 DEG C of temperature
The lower immersion of degree 12 hours, with being drained after distillation water washing；Again plus 1:10 2.8% acetic acid solution, at 8 DEG C, mixing speed
Stir 48 hours under conditions of 3500rpm, centrifuged under conditions of 40000 turns/min；Supernatant is taken to add 1mol/L sodium sulphate, warp
Precipitation, filtering, distillation water washing are crossed, then adds 1:10 2.8% acetic acid solution dissolving, surpassing Yu's membrane separation technique using Guangzhou has
The milipore filter ultrafiltration that the molecular cut off of limit company supply is 100kDa, filtrate freeze-drying, obtain sharkskin and cartilage mixing
Collagen, then take the dissolving of 2% acetic acid solution to be made into the solution that the protein concentration of original containing epoxy glue is 0.3%；
B, the solution for taking step A to prepare, under conditions of low whipping speed 350rpm, sodium alginate 0.6% is added；Add poly-
The weight ratio of PAA, sodium alginate and Sodium Polyacrylate is 1:0.25；Add pigskin collagen, sodium alginate and pigskin
The weight ratio of collagen is 1:0.07, stir 20 minutes；
C, bFGF growth factors are taken using distilled water as solvent, under conditions of low whipping speed 350rpm, prepare chitosan season
Ammonium salt and growth factor composite solution, chitosan quaternary ammonium salt content is that 0.35%, bFGF growth factor contents are in solution
0.001%, stir 10 minutes；
D, using in step B obtained by alginate composite solution be used as the outer layer solution of spinning, with resulting in step C
Internal layer solution as spinning of chitosan-containing quaternary ammonium salt and growth factor composite solution, coaxial electrostatic spinning is carried out, in voltage
It is 18cm for the distance between 30kV, fixed anode and negative electrode, under conditions of temperature is 28~30 DEG C, carries out coaxial electrostatic spinning
Silk, obtains filament faint yellow, with core shell structure and the alginate fiber film being made up of filament on receiver board；
E, using distilled water as solvent, using calcium chloride and zinc chloride, the chlorine containing 3% calcium chloride and 0.5% zinc chloride is prepared
Compound mixed liquor, the nano fibrous membrane obtained by D steps is immersed in mixed liquor, then, take out drying, it is 28 to immerse standing time
Minute, 36 DEG C of hot-air is dried, and drying time is 1.2 hours；
F, each 10 grams of the bletilla striata, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle, Asian puccoon Chinese medicine are taken, adds 120 grams of water, after soaking 20 minutes,
Boil 25 minutes, pour out liquid, the dregs of a decoction add water 100 grams, boil again 20 minutes, merge liquid twice, add the milli of water constant volume 200
Rise, turn into compound Chinese medicine water cooking liquid；Cross-linked alginate compound made from E steps is taken to immerse compound Chinese medicine water cooking liquid, then
Drying is taken out, it is 2 minutes to immerse herbs ' water-decoctum standing time, and hot blast condition is the hot-air of 36 DEG C of temperature, and drying time is
1.5 hour；
G, the epoxy natural rubber for taking epoxidation level to be 30%, is dissolved in tetrahydrofuran, is configured to 1% solution,
Then cleaning is coated on not in sticky paper by the tape casting, 68 DEG C of hot-air is dried, and drying time is 1 hour, the thickness of acquisition
For 0.06 millimeter of epoxy natural rubber film；
H, it is compound
The epoxy natural rubber Film laminated that the compound that step F is obtained obtains with step G, that is, obtain described control
Treat the alginate dressing of bedsore.
Claims (9)
- A kind of 1. alginate medical dressing for treating bedsore, it is characterised in that：The dressing has two-layer composite, i.e., by Bottom and surface layer are formed, and bottom is alginate composite film, and surface layer is natural rubber film layer；The natural rubber film layer is ring Degree of oxidation reaches 30~40% epoxy natural rubber material film, and the thickness of the film is 0.04~0.1 millimeter；The sea Alginates composite film be by sodium alginate, Sodium Polyacrylate, shark collagen, pigskin collagen, chitosan quaternary ammonium salt, The tunica fibrosa that basic fibroblast growth factor (bFGF) different materials are formed, the thickness of the film is 1~5 millimeter；The fibre It is to be arranged under the overlay to form by filament to tie up film, and the filament has core, shell structure, and wherein shell is by sodium alginate, polyacrylic acid Sodium, shark collagen, pigskin collagen material are formed；Sandwich layer is made up of chitosan quaternary ammonium salt and growth factor material, fine Tie up the material layer of film surface coated with chloride containing calcium and zinc chloride and the bletilla by processing, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle and The material layer of the extract of Asian puccoon.
- 2. the preparation method of the alginate medical dressing for the treatment of bedsore as claimed in claim 1, it is characterised in that including such as Lower step：A, shark collagen solution is preparedThe mixture dry product of sharkskin and bone is taken, a certain amount of NaOH solution immersion is added after crushing, is drained, adds petroleum ether immersion Afterwards, drain, after adding acetic acid solution extraction, centrifugation, take supernatant to add metabisulfite solution to precipitate, sediment is washed through distillation Wash, be freeze-dried, obtain sharkskin and bone mixing collagen, be dissolved in certain density acetic acid solution, obtain shark Collagen solution；B, coaxial electrostatic spinning outer layer solution is preparedThe solution for taking step A to obtain, sodium alginate, Sodium Polyacrylate, pigskin collagen are added, is uniformly mixed, obtains A kind of composite solution containing alginate mixture；C, coaxial electrostatic spinning sandwich layer solution is preparedChitosan quaternary ammonium salt is dissolved in distilled water, basic fibroblast growth factor (bFGF) is added, stirs, obtain To a kind of composite solution containing chitosan quaternary ammonium salt and growth factor；D, spinningUsing coaxial electrostatic spinning method, the alginate composite solution for allowing step B to obtain is molten as the outer layer of coaxial electrostatic spinning Liquid, allow step C obtains contain chitosan quaternary ammonium salt and the composite solution of growth factor is molten as the internal layer of coaxial electrostatic spinning Liquid, coaxial spinning is carried out, obtain the filament with nucleocapsid double-decker based on alginate and be made up of filament Tunica fibrosa；E, it is crosslinkedThe tunica fibrosa that step D is obtained immerses some time, chloride in chloride mixture solution and uses calcium chloride and chlorine Change zinc, dry some time after taking-up under the conditions of hot blast, obtain cross-linked alginate compound；F, impregnateThe cross-linked alginate compound that step E is obtained is immersed into answering containing the bletilla striata, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle and Asian puccoon composition Close in herbs ' water-decoctum and place some time, take out and certain time is dried under the conditions of hot blast, obtain the crosslinking containing traditional Chinese medicine ingredients Alginate compound；G, epoxy natural rubber film is preparedThe tape casting is used to prepare thickness as 0.04~0.1 millimeter of epoxy natural rubber film；H, it is compoundThe epoxy natural rubber Film laminated that the compound that step F is obtained obtains with step G, that is, obtain described treatment cotton-padded mattress The alginate dressing of sore.
- 3. preparation method according to claim 2, it is characterised in that：The mixture dry product of sharkskin and bone is in step A The weight of sharkskin and Shark cartilage ratio is 6~7:4~3 mixtures, by drying to water content weight than dry less than 5% Product；It is 1 that the dry product, which is crushed after fineness 20-50 mesh, to add weight ratio,:The 0.1N/L NaOH solutions of 15 mass, at a temperature of 8 DEG C Immersion 5 hours, taking-up drains；It is 1 to add weight ratio:10 petroleum ether, soak 12 hours at a temperature of 8 DEG C, with distillation water washing After drain；Add weight ratio is 1 again:10 2.5%-3.5% weight than acetic acid solution and at 8 DEG C, mixing speed be 300~ 500rpm is stirred 48 hours；Centrifuged by 40000 turns/min, take supernatant to add 1mol/L sodium sulphate, by precipitating, filtering, Water washing is distilled, then add weight ratio is 1:10 2.5%-3.5% weight than acetic acid solution dissolving, use molecular cut off for 100kDa milipore filter ultrafiltration, filtrate freeze-drying, obtains sharkskin and cartilage mixing collagen, then it is 2% second to take weight ratio Acid solution dissolve, be made into the protein concentration of original containing epoxy glue for 0.3% weight than solution.
- 4. preparation method according to claim 2, it is characterised in that：Sodium alginate addition weight described in step B Than for 0.4~0.8%, the weight ratio of sodium alginate and Sodium Polyacrylate is 1:0.2~0.4, sodium alginate and pigskin collagen Weight ratio is 1:0.05~0.08, the pigskin collagen is low molecule collagen of the molecular weight in 10D to 30D, and stirring is fast Spend for 300~500rpm, stirring 20 minutes.
- 5. preparation method according to claim 2, it is characterised in that：Chitosan quaternary ammonium salt and growth described in step C Chitosan quaternary ammonium salt in factor composite solution is the quaternised modified product of chitosan, and content weight ratio is 0.3~0.5%； Basic fibroblast growth factor (bFGF) content weight ratio is 0.001%, and mixing speed is 300~500rpm, stirring 10 Minute.
- 6. preparation method according to claim 2, it is characterised in that the coaxial electrostatic spinning method described in step D, be with Outer layer solution of the alginate composite solution as spinning obtained by step B, with chitosan-containing season resulting in step C The internal layer solution of ammonium salt and growth factor composite solution as spinning, voltage between 30kV, fixed anode and negative electrode away from From for 18cm, under conditions of temperature is 28~30 DEG C, carry out coaxial electrostatic spinning, obtained on receiver board it is faint yellow, with core The filament of shell structure and the alginate fiber film being made up of filament.
- 7. preparation method according to claim 2, it is characterised in that：Chloride mix solution described in step E is Containing weight than be 3% calcium chloride and weight than the mixed aqueous solution for 0.5% zinc chloride, standing time is 20 to 30 points after immersion Clock, then take out, dried in 35~40 DEG C of hot-airs, drying time is 1~2 hour.
- 8. preparation method according to claim 2, it is characterised in that：Compound Chinese medicine water cooking liquid described in step F, is Chinese medicine The bletilla striata, the radix paeoniae rubrathe, dragon's blood, building soft-shelled turtle and each 10 grams of Asian puccoon, 120 grams of water are added, after soaking 20 minutes, boil 25 minutes, pour out liquid Body, the dregs of a decoction add water 100 grams, boil again 20 minutes, merge liquid twice, add 200 milliliters of water constant volume, turn into compound Chinese medicine water Boil liquid；Standing time is 2 minutes after cross-linked alginate compound immerses herbs ' water-decoctum, drying is then taken out, 35~40 Dried in DEG C hot-air, drying time is 1~2 hour.
- 9. preparation method according to claim 2, it is characterised in that：Epoxy natural rubber film described in step G The epoxy natural rubber for being 30~40% for epoxidation level, is dissolved in tetrahydrofuran, and it is molten for 1% to be configured to weight ratio Liquid, cleaning is then coated on not in sticky paper by the tape casting, by 68~70 DEG C of hot-airs drying, drying time is 40 points Clock, the thickness of acquisition are 0.04~0.1 millimeter of epoxy natural rubber film.
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| CN110237295A (en) * | 2019-07-29 | 2019-09-17 | 河南亚都实业有限公司 | A kind of anthocyanidin alginate dressing |
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| CN106729939B (en) * | 2016-12-29 | 2018-01-12 | 河南亚都实业有限公司 | A kind of dressing for treating bedsore |
| CN107237005B (en) * | 2017-06-19 | 2020-06-09 | 山东大学 | Alginic acid and chitosan double-component fiber and preparation method thereof |
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| CN109908395A (en) * | 2018-08-17 | 2019-06-21 | 上海昊海生物科技股份有限公司 | A kind of sharkskin natural medical collagen protein sponge preparation method |
| CN109498830A (en) * | 2018-10-22 | 2019-03-22 | 江阴奔翔生物科技有限公司 | A kind of method of the sodium alginate-modified acellular dermal matrix of epoxy |
| CN109731057A (en) * | 2019-01-17 | 2019-05-10 | 北京化工大学 | A kind of tunica fibrosa and preparation method thereof of sustainable release dragon's blood |
| CN110353883A (en) * | 2019-07-10 | 2019-10-22 | 重庆市人民医院 | For treating the combine dressing layer of bedsore and the bedsore with combine dressing layer is pasted |
| CN110960722A (en) * | 2019-12-14 | 2020-04-07 | 广东泰宝医疗科技股份有限公司 | Antibacterial alginate bandage and preparation method thereof |
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| DE3378185D1 (en) * | 1982-07-21 | 1988-11-17 | Univ Strathclyde | Composite wound dressing |
| US20040153040A1 (en) * | 2003-02-05 | 2004-08-05 | Lucie Martineau | Multi-layer synthetic dressing with cooling characteristics |
| CN100518835C (en) * | 2006-12-27 | 2009-07-29 | 褚省吾 | Preparing method of liquid hemostatic silicone rubber dressing |
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