CN104758977A - An alginate dressing for treating bedsore and a preparing method thereof - Google Patents
An alginate dressing for treating bedsore and a preparing method thereof Download PDFInfo
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Abstract
The invention relates to an alginate dressing for treating bedsore and a preparing method thereof. The dressing has a double-layer composite structure, namely the dressing comprises a base layer and a surface layer. The base layer is an alginate composite film layer. The surface layer is a natural rubber film layer. The preparing method includes following steps of: A) preparing a shark collagen solution, B) preparing a coaxial electrospinning outer layer solution, C) preparing a coaxial electrospinning core layer solution, D) spinning, E) crosslinking, F) dipping, G) preparing an epoxy natural rubber film, and H) compositing. The dressing has good biocompatibility, and has controllable growth regulation, good hygroscopicity, good anti-inflammation performance and good antibacterial performance of synthetic biological materials. The dressing is capable of improving local blood supply of wounds, promoting tissue growth and accelerating wound healing, shows good multifunctional properties, has good porosity, soft and comfort hand feeling, good compliance and using convenience, and can be used in the field of modern biomedicine, especially in the field of bedsore treatment and nursing, and the like.
Description
Technical field
The invention belongs to medical product technical field, more specifically, the present invention relates to a kind of alginate medical dressing for the treatment of decubital ulcer, also relate to the preparation method of described dressing.
Background technology
Decubital ulcer is also known as pressure ulcer or pressure ulcer, it is one of common complication of internal medicine, due to neural and disturbance of blood circulation, local organization continuous ischemia, malnutrition and the soft tissue necrosis that occurs, it is the complicated pathological process that body causes by endogenous cause of ill and exopathogenic factor combined effect, it is a kind of disease of very refractory, once occur, the course for the treatment of is long, easy recurrence, can cause from epidermis to subcutaneous tissue, muscle, the even destruction of B&J, bring great misery to patient, severe patient can secondary infection, causes septicemia and causes death.
In the U.S., there is decubital ulcer complication in the inpatient of 4.3%, its annual medical expense is up to multi-million dollar, Britain also spends several hundred million pounds every year and is used for the treatment of patients with bedsore, China there is no complete statistics in this respect, but because China is populous, bed patient is many, decubital ulcer sickness rate is also in high numerical value always.For treating patients with decubitus, all do a large amount of research work both at home and abroad, but up to now, still do not find medicine and the method for very effective treatment and nursing.Due in following a series of Diseases, comprise I, II, III and IV phase diabetics, stupor is caused after brain injury, hemiplegia, hemiplegia or paralytic patient, fracture-dislocation of cervical spine merges paralytic patient, thoracic spine fracture and dislocation merges paraplegia, burst fracture of lumbar merges paralytic patient, osteoporosis causes fracture patient, the patient etc. of longer-term bed in late tumor patient and other affected treatment, there is the concurrent decubital ulcer situation of a large amount of appearance, therefore, the prophylactic treatment of decubital ulcer and nursing become an arduousness and important work, also be the medium-term and long-term existence of nursery work and a great problem do not solved completely.
At present, the method that clinical treatment decubital ulcer is conventional comprises therapeutic method of surgery, drug treatment and outer coating method.Operative treatment mainly adopts transfer of skin flap reparation after thorough debridement, but the method is not suitable for for many patients; Pharmacotherapy, comprises oral medicine and medicine for external use, also ubiquity effect not significantly shortcoming.
Because outer coating method is simple to operation, and minimum to the injury of patient, therefore, very big concern is also given in many clinical researches.Tradition wound dressing comprise cotton yarn binder, binder/package binder, specialized dressing, dipping cotton yarn, slit close bar alternate product and emergency processing dressing sundry goods etc., major embodiment hemostasis, fungi-proofing, prevent wound adhesion and simply wrap up function.Obviously, traditional wound dressing cannot meet the demands in treating patients with decubitus.
Therefore, some research work also pay close attention to the exploitation of decubital ulcer specialized dressing and Therapeutic Method, and changing dressings new method as: patent publication No. CN1593675A proposes a kind of decubital ulcer, is with 2% acetic acid wash bedsore wound, cover with Triterpene acid gauze again and wrap up with aseptic dressing, obtain therapeutic effect; Patent publication No. CN101461960A proposes a kind of bedsore biological dressing, its main component is chitosan, polyhexamethylene guanidine, Alumen, two blooms, Oleum sesami, Gan Bo etc. are mixed, and for the anti-inflammation hemostasia of various wound, antibiotic and sterilizing, dry and comfortable anti, promotes skin histology healing and repairs; Patent CN publication number 102885859A proposes a kind of medicine for the treatment of decubital ulcer, pasty state is mixed into by Dragon bone(fried) powder, YUNNAN BAIYAOFEN, 75% ethanol, first with hydrogen peroxide cleaning decubital ulcer skin ulcer face in treatment, rear far IR curing instrument irradiates, then is spread upon by medicine on skin ulcer face and play therapeutical effect; Patent CN publication number 103271794A proposes a kind of biological antibiotic based on bedsore wound and applies ointment or plaster, this compositions such as comprising chitosan, acetic acid, lysozyme, gelatin, chlorhexidine acetate, aerosil and water of applying ointment or plaster, be made up of ventilative base material, non-woven synthetic's silk, biological antibiotic layer, papery sealing coat, be used for the treatment of bedsore wound and burn and scald, Postoperative infection.
Research shows, keeps the moist environment of bedsore wound, control oxygen and water through situation, form airtight microenvironment, and possess can composite for born muscle antiphlogosis, Promote cell's growth condition, for treating, decubital ulcer is most important.Obviously, several above-mentioned decubital ulcer patent major parts just adopt compound and the simple therapy mode of alginate, some Chinese and western drugses, simple material simply, do not further consider how design effectively new pattern compress, by considering physics and chemistry, biological factor, keep the moistening of pressurized position microenvironment on the one hand, control wound surface effluent, stablize wound surface variations in temperature; On the other hand, control the transmitance of oxygen and steam, maintain the partial pressure of oxygen that pressurized position is suitable, promote that microcirculation is formed; Consider simultaneously antibacterial with protect from infection, skin surface construct one deck defense against bacterial invade barrier, strengthen tissue anti-inflammatory power; Then, further consider the composition utilizing and have and promote wound healing, the growth of fibroblast and endotheliocyte and promotion keratinocyte proliferation can be stimulated, reach strengthening wound healing and the object promoting therapeutic effect.
At present, the high-end dressing being used for the treatment of decubital ulcer that China market has independent intellectual property right is also little, and can consider that to maintain bedsore wound moistening with oxygen, moisture microenvironment, adopt the anti-infection of high-efficiency antimicrobial and further considering be applied to can the material of accelerating wound healing to prepare modern decubital ulcer dressing, be almost blank.Therefore, study novel high-end decubital ulcer dressing technology of preparing and preparation method, be extremely important.
Summary of the invention
The object of this invention is to provide alginate dressing of a treatment decubital ulcer and preparation method thereof, its therapeutic effect is good, easy to prepare, is convenient to promote.
For achieving the above object, the technical scheme that the present invention takes is:
Treat an alginate medical dressing for decubital ulcer, this dressing has two-layer composite, is namely made up of bottom and surface layer, and bottom is alginate composite film, and surface layer is natural rubber rete.
1. natural rubber rete described in is the epoxy natural rubber material film that epoxidation level reaches 30 ~ 40%, and the thickness of this film is 0.04 ~ 0.1 millimeter; Described alginate composite film is the fibrous membrane be made up of sodium alginate, sodium polyacrylate, shark collagen protein, pigskin collagen, chitosan quaternary ammonium salt, basic fibroblast growth factor (bFGF) different materials, and the thickness of this film is 1 ~ 5 millimeter; Described fibrous membrane is arranged under the overlay by cellosilk to form, and this cellosilk has core, shell structure, and wherein, shell is made up of sodium alginate, sodium polyacrylate, shark collagen protein, pigskin collagen material; Sandwich layer is made up of chitosan quaternary ammonium salt and somatomedin material, the material layer of the extract of the bletilla that fibrous membrane surface-coated has the material layer of chloride containing calcium and zinc chloride and process to concoct, Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, Radix Arnebiae (Radix Lithospermi).
Treat a preparation method for the alginate medical dressing of decubital ulcer, comprise the steps:
A, preparation shark collagen solution
Get the mixture dry product of sharkskin and bone, add a certain amount of NaOH solution after pulverizing to soak, drain, after adding petroleum ether immersion, drain, add again acetic acid solution extract, centrifugal after, get supernatant and add metabisulfite solution precipitation, precipitate, through distilled water wash, lyophilization, obtains sharkskin and bone mixing collagen protein, be dissolved in certain density acetic acid solution, obtained shark collagen solution.
B, prepare the outer solution of coaxial electrostatic spinning
Get the solution that steps A obtains, add sodium alginate, sodium polyacrylate, pigskin collagen, be uniformly mixed, obtain a kind of composite solution containing alginate mixture;
C, prepare coaxial electrostatic spinning sandwich layer solution
Chitosan quaternary ammonium salt is dissolved in distilled water, adds basic fibroblast growth factor (bFGF), stir, obtain a kind of composite solution containing chitosan quaternary ammonium salt and somatomedin;
D, spinning
Adopt coaxial electrostatic spinning method, the alginate composite solution allowing step B obtain is as the outer solution of coaxial electrostatic spinning, the internal layer solution of composite solution as coaxial electrostatic spinning containing chitosan quaternary ammonium salt and somatomedin allowing step C obtain, carry out coaxial spinning, obtain based on alginate with the double-deck cellosilk of nucleocapsid and the fibrous membrane that is made up of cellosilk;
E, crosslinked
The fibrous membrane that obtained by step D immerses the some time in chloride mixture solution, after taking-up under hot blast condition the dry some time, obtain cross-linked alginate complex;
F, dipping
Cross-linked alginate complex step e obtained immerses in the compound Chinese medicine water cooking liquid containing the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, Radix Arnebiae (Radix Lithospermi) composition places the some time, take out dry certain hour under hot blast condition, obtain the cross-linked alginate complex containing Chinese medicine ingredients;
G, prepare epoxy natural rubber thin film
The tape casting is adopted to prepare the epoxy natural rubber thin film that thickness is 0.04 ~ 0.1 millimeter;
H, compound
The epoxy natural rubber Film laminated that the complex obtain step F and step G obtain, namely obtains the alginate dressing of described treatment decubital ulcer.
In preferred steps A, the mixture dry product of sharkskin and bone is that the ratio of sharkskin and shark cartilage is respectively 6 ~ 7:4 ~ 3 mixture, through being dried to water content lower than 5% dry product; This dry product is pulverized as after fineness 20-50 order, adds the 0.1N/L NaOH solution of 1:15 quality, and soak 5 hours at 8 DEG C of temperature, taking-up drains; Add the petroleum ether of 1:10, soak 12 hours at 8 DEG C of temperature, drain with after distilled water wash; Add the acetic acid solution of the 2.5%-3.5% of 1:10 again and at 8 DEG C, mixing speed is that 300 ~ 500rpm stirs 48 hours; Centrifugal through 40000 turns/min, get supernatant and add 1mol/L sodium sulfate, through precipitation, filtration, distilled water wash, the acetic acid solution adding the 2.5%-3.5% of 1:10 again dissolves, adopt molecular cut off to be the ultrafiltration of 100kDa ultrafilter membrane, filtrate lyophilization, obtain sharkskin and cartilage mixing collagen protein, get 2% acetic acid solution again to dissolve, being made into containing the former protein concentration of epoxy glue is the solution of 0.3%
Sodium alginate addition described in preferred step B is 0.4 ~ 0.8%, the weight ratio of sodium alginate and sodium polyacrylate is 1:0.2 ~ 0.4, the weight ratio of sodium alginate and pigskin collagen is 1:0.05 ~ 0.08, this pigskin collagen is the low molecule collagen protein of molecular weight at 10D to 30D, mixing speed is 300 ~ 500rpm, stirs 20 minutes.
Chitosan quaternary ammonium salt in chitosan quaternary ammonium salt described in preferred step C and somatomedin composite solution is the quaternised modified product of chitosan, and content is 0.3 ~ 0.5%; Basic fibroblast growth factor (bFGF) content is 0.001%, and mixing speed is 300 ~ 500rpm, stirs 10 minutes.
Coaxial electrostatic spinning method described in preferred step D, the alginate composite solution that obtains in the step B outer solution as spinning, the chitosan-containing quaternary ammonium salt obtained in step C and somatomedin composite solution are as the internal layer solution of spinning, voltage be 30kV, distance between fixed anode and negative electrode is 18cm, temperature is under the condition of 28 ~ 30 DEG C, carry out coaxial electrostatic spinning, dash receiver obtains faint yellow, with the cellosilk of nucleocapsid structure and the alginate fiber film be made up of cellosilk.
Chloride mix solution described in preferred step e is the mixed aqueous solution containing 3% calcium chloride and 0.5% zinc chloride, and after immersing, standing time is 20 to 30 minutes, then takes out, and dry in 35 ~ 40 DEG C of hot-airs, drying time is 1 ~ 2 hour.
Compound Chinese medicine water cooking liquid described in preferred step F, bletilla, Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, each 10 grams of Radix Arnebiae (Radix Lithospermi), add 120 grams of water, soak after 20 minutes, boil 25 minutes, pouring liquids, medicinal residues add water 100 grams again, again boil 20 minutes, merge twice liquid, add water standardize solution 200 milliliters, becomes compound Chinese medicine water cooking liquid; After cross-linked alginate complex immersion herbs ' water-decoctum, standing time is 2 minutes, then takes out drying, and dry in 35 ~ 40 DEG C of hot-airs, drying time is 1 ~ 2 hour.
Epoxy natural rubber thin film described in preferred step G to be epoxidation level be 30 ~ 40% epoxy natural rubber, be dissolved in oxolane, be mixed with 1% solution, then cleaning is coated on not in sticky paper by the tape casting, through the hot-air dry of 68 ~ 70 DEG C, drying time is 40 minutes, and the thickness of acquisition is the epoxy natural rubber thin film of 0.04 ~ 0.1 millimeter.
The sharkskin that the present invention is used and shark cartilage are product solds in the market, such as by Beihai Zhen Meida marine organisms Products Co., Ltd with trade name shark cartilage powder and shark corium farinosum product sold.
The ultrafilter membrane that the present invention uses is product sold in the market, such as by Beijing Tao Shi ultrafilter membrane company supply with trade name SFP2680 ultrafilter membrane or Guangzhou Chao Yu membrane separation technique company limited with trade name CYG90S polysulphones hyperfiltration membrane product sold.
If the shark cartilage content that the present invention uses lower than 30% time, then can make the antioxidation of dressing, free radical resisting and antiinflammation reduce; If the present invention use shark cartilage content higher than 40% time, then there will be Anti-angiogenesis factors too high levels, unfavorable wound healing.Therefore, the shark cartilage content that the present invention uses be 30 ~ 40% is suitable, preferably 32 ~ 38%, more preferably 34 ~ 36%.
The sodium alginate that the present invention uses for being product sold in the market, such as by Qingdao Huanghai Sea bio-pharmaceuticals Co., Ltd with trade name sodium alginate product sold or by Lianyun Harbour everyday seaweed industry company limited with trade name sodium alginate product sold.
The sodium polyacrylate that the present invention uses is product sold in the market, such as by Shandong KeYu water treatment company limited with trade name sodium polyacrylate or by Jiaozuo City Tai Feng Fine Chemical Co., Ltd with trade name sodium polyacrylate product sold.
The pigskin collagen that the present invention uses is the pigskin collagen prepared by Ji'nan University's biomedical engineering institute patented technology (CN1470640).
If the present invention use sodium alginate content lower than 0.4% time, then follow-up electrostatic spinning can be made to carry out; If the present invention use sodium alginate content higher than 0.8% time, then there will be dissolving and be not easy problem.Therefore, the sodium alginate content that the present invention uses be 0.4 ~ 0.8% is suitable, preferably 0.5 ~ 0.8%, more preferably 0.6 ~ 0.7%.
If the present invention use sodium polyacrylate content lower than 0.2 time, then the water absorption of the dressing can prepared affects adversely; If the sodium polyacrylate content that the present invention uses higher than 0.4, then can make the dissolving of follow-up collagen protein influenced.Therefore, the sodium polyacrylate content that the present invention uses be 0.2 ~ 0.4 is suitable, preferably 0.3 ~ 0.4%, more preferably 0.4%.
If the present invention use pigskin collagen content lower than 0.05 time, then cannot play the effect of nursing skin and wound; If the present invention use collagen content higher than 0.08 time, then there will be the problem affecting electrostatic spinning.Therefore, the collagen content that the present invention uses be 0.05 ~ 0.08 is suitable, preferably 0.06 ~ 0.08, more preferably 0.07 ~ 0.08.
The chitosan quaternary ammonium salt that the present invention uses is product sold in the market, such as by Nantong Lv Shen biological engineering company limited with trade name chitosan quaternary ammonium salt product sold.
The bFGF somatomedin that the present invention uses is the bFGF somatomedin prepared by Bio-engineering Institute of Jinan University's patented technology (CN1500809).
If the present invention use chitosan quaternary ammonium salt content lower than 0.3% time, then can make the antibacterial and anti-inflammation functions that dressing cannot embody; If the chitosan quaternary ammonium salt content that the present invention uses higher than 0.5% time, then have influence on somatomedin and to bring into normal play usefulness.Therefore, the chitosan quaternary ammonium salt content that the present invention uses is 0.3 ~ 0.5% is suitable, is preferably 0.4 ~ 0.5%.
The calcium chloride that the present invention uses for being product sold in the market, such as by chemical industry company limited of Shandong Eastcom with trade name calcium chloride product sold or by Divine Land, Weifang City Chemical Co., Ltd. with trade name calcium chloride product sold.
The zinc chloride that the present invention uses is product sold in the market, such as by Ji, Jinzhou City, Hebei province Tian Xinye company limited with trade name zinc chloride or by Guangzhou Reagent Company with trade name zinc chloride product sold.
If when the time in the present invention in fibrous membrane immersion mixed liquor was lower than 20 minutes, then fibrous membrane can be made because of crosslinked deficiency to affect the performance of dressing; If when the time in fibrous membrane immersion mixed liquor was higher than 30 minutes, then can reduce the imbibition effect of dressing because of excessively crosslinked.Therefore, immersion standing time that the present invention adopts is 20 to 30 minutes, is most preferably 25 minutes.
The Chinese herbal medicine Pseudobulbus Bletillae (Rhizoma Bletillae) that the present invention uses, Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, Radix Arnebiae (Radix Lithospermi) are product solds in the market, such as by north and south, Guangzhou row prepared slices of Chinese crude drugs company limited with the trade name Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, Radix Arnebiae (Radix Lithospermi) product sold.
The epoxy natural rubber that the present invention uses is the epoxy natural rubber produced by Chinese Academy of Tropical Agricultural Sciences's Product processing institute patented technology (CN101654488).
If the present invention use epoxy natural rubber epoxidation level lower than 30% time, then can affect the obturation effect of dressing; If the present invention use epoxy natural rubber epoxidation level higher than 40% time, then there will be banana too low, steam is not easy to distribute, and affects the facilitation of dressing to wound healing.Therefore, the epoxidation level of epoxy natural rubber that the present invention uses is 30 ~ 40% is suitable, preferably 31 ~ 37%, more preferably 32 ~ 35%.
In above step, article dry in hot-air dry equipment reach water content lower than by weight less than 5%.The centrifugation apparatus used in above step, drying equipment be normally used in the art, at product sold in the market.The water content of described article adopts dry Weighing method to measure and obtains.The electrospinning device that the present invention uses is the rotary drum electrostatic spinning machine adopting Ji'nan University's biomedical engineering institute patent (CN103060932A) technology to manufacture.
The invention has the beneficial effects as follows:
1. adopt shark collagen protein as the ingredient of dressing, both the good antioxidation of sharkskin collagen, free radical resisting and antiinflammatory and promotion bedsore wound Healing had been utilized, use again the angiostatin active component of shark cartilage collagen and polysaccharide, control wound surface and can not produce hamartoplasia at agglutination, without obvious cicatrization.
2. adopt sodium alginate and sodium polyacrylate as the main part of dressing, both utilized the good absorbent of alginate and promoted skin growth, utilize again the high-hygroscopicity of sodium polyacrylate, ensure that wound surface is in moist environment always, be of value to the healing of wound surface.Meanwhile, also utilize Corii Sus domestica low molecule collagen protein further, play the effect promoting the healing of wound surface health.
3. utilize coaxial spining technology, using chitosan quaternary ammonium salt and somatomedin as inner nuclear layer, produce slow releasing function, both played anti-inflammation, and can promote that again skin grew; Adopt electrostatic spinning technique, by the spinned fiber of alginate and shark collagen, low molecule collagen, control fibre structure, adjustment breathability, and form good microbial barrier, the performance of dust.In addition, the nanofiber of preparation has multifunctionality, possesses up to 100m
2the specific surface area of/more than g, has good imbibition hygroscopicity, effectively can absorb the effluent of wound, can ensure the biotic environment of wound surface, be conducive to the healing of wound surface, alleviate the misery of patient.
4. adopt zinc to be combined with calcium divalent ion, as cross-linking agent, make part sodium alginate pass through ion exchange, generate divalent ion crosslink complex, make dressing have good physical and mechanical properties and water indissolubility energy, meanwhile, utilize dressing flap coverage and contact, produce ion exchange, discharge zinc ion, zinc ion permeates at wound surface, accelerates epidermis cell and moves, and improve the regeneration capacity of cell, wound healing.
5. the compound Chinese medicine water cooking liquid impregnated dressings containing the Chinese herbal medicine Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, Radix Arnebiae (Radix Lithospermi) composition is adopted, the effect utilizing the astringing to arrest bleeding that these Chinese medicines are good, dissipating blood stasis of subsiding a swelling, blood activating and promoting tissue regeneration, the removing toxic substances for the treatment of skin ulcer and wound healing to grow, improves healing speed further.
6. research shows, the dressing of application seal effectively can completely cut off the intrusion of extraneous antibacterial, significantly reduce infection rate, and the wound surface spread of germs and cause nosocomialtion of protecting from infection, simultaneously, containing abundant macrophage, lymphocyte, mononuclear cell etc. in the wound fluid that closed environment stores, the host phagocytes being conducive to leukocyte mediation plays a role, and strengthens local bactericidal and tissue regeneration ability.The present invention utilizes the good air-tightness of epoxidezed rubber, there is the effect reconciling oxygen and water vapor permeability rates, thickness is adopted to be that the epoxy natural rubber thin film of 0.04 ~ 0.1 millimeter is as outermost layer, maintain the relatively airtight and moist environment of wound surface, strengthen leukocyte function, accelerate the epithelization of wound surface, granulation formed, the degraded of cellulose and downright bad material, the breeding of anti-bacteria and diffusion, thus the time greatly shortening wound healing.
Dressing prepared by the present invention is a kind of complex, has the machinery of modern dressing, physics and chemistry, biology performance, the good biocompatibility of existing natural biologic material, has again the controllable growth of synthesising biological material to reconcile, good hygroscopicity and antiinflammatory, antibiotic property, the supply of blood flow of wound local can be improved, promote tissue growth, accelerate wound healing, demonstrate good multifunctionality, have good porosity, soft comfortable, compliance is good simultaneously, easy to use, be adapted at modern biomedical field, particularly treating patients with decubitus and the field such as to nurse and use, alginate dressing prepared by the present invention can be applicable to comprise I, II, III and IV phase diabetics, stupor is caused after brain injury, hemiplegia, hemiplegia or paralytic patient, fracture-dislocation of cervical spine merges paralytic patient, thoracic spine fracture and dislocation merges paraplegia, burst fracture of lumbar merges paralytic patient, osteoporosis causes fracture patient, the purposes such as the treatment of the concurrent decubital ulcers such as the patient of longer-term bed in late tumor patient and other affected treatment and nursing.
Accompanying drawing explanation
Fig. 1 is the structural representation of embodiment of the present invention alginate medical dressing.
Fig. 2 is the filametntary structural representation of the embodiment of the present invention.
Detailed description of the invention
Embodiment 1
2. as shown in Figure 1 and Figure 2, a kind of alginate medical dressing for the treatment of decubital ulcer, this dressing has two-layer composite, is namely made up of bottom and surface layer, and bottom is alginate composite film 2, and surface layer is natural rubber rete 1.Natural rubber rete 1 reaches the epoxy natural rubber material film of 30 ~ 40% for epoxidation level, and the thickness of this film is 0.04 ~ 0.1 millimeter; Alginate composite film 2 is the fibrous membranes be made up of sodium alginate, sodium polyacrylate, shark collagen protein, pigskin collagen, chitosan quaternary ammonium salt, basic fibroblast growth factor (bFGF) different materials, and the thickness of this film is 1 ~ 5 millimeter; Fibrous membrane is arranged under the overlay by cellosilk to form, and this cellosilk has core 4, shell 3 structure, and wherein shell is made up of sodium alginate, sodium polyacrylate, shark collagen protein, pigskin collagen material; Sandwich layer is made up of chitosan quaternary ammonium salt and somatomedin material, the material layer of the extract of the bletilla that fibrous membrane surface-coated has the material layer of chloride containing calcium and zinc chloride and process to concoct, Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, Radix Arnebiae (Radix Lithospermi).
Treat a preparation method for the alginate medical dressing of decubital ulcer, comprise the steps:
A, to get smashing fineness be the content ratio of 20 object sharkskins and shark cartilage is the mixture dry product of 6.7:3.4, and add the 0.1N/L NaOH solution of 1:15 quality, soak 5 hours at 8 DEG C of temperature, taking-up drains; Add the petroleum ether of 1:10, soak 12 hours at 8 DEG C of temperature, drain with after distilled water wash; Add the acetic acid solution of 2.5% of 1:10 again, at 8 DEG C, stir 48 hours under the condition of mixing speed 300rpm, centrifugal under the condition of 40000 turns/min; Get supernatant and add 1mol/L sodium sulfate, through precipitation, filtration, distilled water wash, the acetic acid solution adding 2.5% of 1:10 again dissolves, the molecular cut off adopting Beijing Tao Shi ultrafilter membrane company to supply is the ultrafilter membrane ultrafiltration of 100kDa, filtrate lyophilization, obtain sharkskin and cartilage mixing collagen protein, then getting 2% acetic acid solution dissolving, to be made into containing the former protein concentration of epoxy glue be the solution of 0.3%;
B, get solution prepared by step A, under the condition of low whipping speed 300rpm, add sodium alginate 0.4%; Add sodium polyacrylate, the weight ratio of sodium alginate and sodium polyacrylate is 1:0.2; Add pigskin collagen, the weight ratio of sodium alginate and pigskin collagen is 1:0.05, stirs 20 minutes;
C, be solvent with distilled water, get basic fibroblast growth factor (bFGF), under the condition of low whipping speed 300rpm, preparation chitosan quaternary ammonium salt and somatomedin composite solution, in solution, chitosan quaternary ammonium salt content is 0.3%, bFGF growth factor content is 0.001%, stirs 10 minutes;
D, the alginate composite solution that obtains in step B are as the outer solution of spinning, the chitosan-containing quaternary ammonium salt obtained in step C and somatomedin composite solution are as the internal layer solution of spinning, carry out coaxial electrostatic spinning, voltage be 30kV, distance between fixed anode and negative electrode is 18cm, temperature is under the condition of 28 ~ 30 DEG C, carry out coaxial electrostatic spinning, dash receiver obtains faint yellow, with the cellosilk of nucleocapsid structure and the alginate fiber film be made up of cellosilk;
E, be solvent with distilled water, adopt calcium chloride and zinc chloride, preparation, containing the chloride mixed liquor of 3% calcium chloride with 0.5% zinc chloride, by the nano fibrous membrane of D step gained immersion mixed liquor, then takes out drying.Immersing standing time is 20 minutes, and at the hot-air dry of 35 DEG C, drying time is 2 hours;
F, get the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, each 10 grams of Radix Arnebiae (Radix Lithospermi) Chinese crude drug, add 120 grams of water, soak after 20 minutes, boil 25 minutes, pouring liquids, medicinal residues add water 100 grams again, again boil 20 minutes, merge twice liquid, add water standardize solution 200 milliliters, becomes compound Chinese medicine water cooking liquid; Get the obtained cross-linked alginate complex of E step and immerse compound Chinese medicine water cooking liquid, then take out drying, immersing herbs ' water-decoctum standing time is 2 minutes, 35 DEG C of hot-air dries, and drying time is 2 hours;
G, get the epoxy natural rubber that epoxidation level is 32%, be dissolved in oxolane, be mixed with 1% solution, then cleaning is coated on not in sticky paper by the tape casting, through the hot-air dry of 68 DEG C, drying time is 2 hours, and the thickness of acquisition is the epoxy natural rubber thin film of 0.04 millimeter;
H, compound
The epoxy natural rubber Film laminated that the complex obtain step F and step G obtain, namely obtains the alginate dressing of described treatment decubital ulcer.
Embodiment 2
Treat a preparation method for the alginate medical dressing of decubital ulcer, comprise the steps:
A, to get smashing fineness be the content ratio of 50 object sharkskins and shark cartilage is the mixture dry product of 6.4:3.6, and add the 0.1N/L NaOH solution of 1:15 quality, soak 5 hours at 8 DEG C of temperature, taking-up drains; Add the petroleum ether of 1:10, soak 12 hours at 8 DEG C of temperature, drain with after distilled water wash; Add the acetic acid solution of 3.5% of 1:10 again, at 8 DEG C, stir 48 hours under the condition of mixing speed 500rpm, centrifugal under the condition of 40000 turns/min; Get supernatant and add 1mol/L sodium sulfate, through precipitation, filtration, distilled water wash, the acetic acid solution adding 3.5% of 1:10 again dissolves, the molecular cut off adopting Guangzhou Chao Yu membrane separation technique company limited to supply is the ultrafilter membrane ultrafiltration of 100kDa, filtrate lyophilization, obtain sharkskin and cartilage mixing collagen protein, then getting 2% acetic acid solution dissolving, to be made into containing the former protein concentration of epoxy glue be the solution of 0.3%.
B, get solution prepared by step A, under the condition of low whipping speed 500rpm, add sodium alginate 0.7%; Add sodium polyacrylate, the weight ratio of sodium alginate and sodium polyacrylate is 1:0.4; Add pigskin collagen, the weight ratio of sodium alginate and pigskin collagen is 1:0.08, stirs 20 minutes;
C, be solvent with distilled water, get bFGF somatomedin, under the condition of low whipping speed 500rpm, preparation chitosan quaternary ammonium salt and somatomedin composite solution, in solution, chitosan quaternary ammonium salt content is 0.5%, bFGF growth factor content is 0.001%, stirs 10 minutes;
D, the alginate composite solution that obtains in step B are as the outer solution of spinning, the chitosan-containing quaternary ammonium salt obtained in step C and somatomedin composite solution are as the internal layer solution of spinning, carry out coaxial electrostatic spinning, voltage be 30kV, distance between fixed anode and negative electrode is 18cm, temperature is under the condition of 28 ~ 30 DEG C, carry out coaxial electrostatic spinning, dash receiver obtains faint yellow, with the cellosilk of nucleocapsid structure and the alginate fiber film be made up of cellosilk;
E, be solvent with distilled water, adopt calcium chloride and zinc chloride, preparation contains the chloride mixed liquor of 3% calcium chloride and 0.5% zinc chloride, the nano fibrous membrane of D step gained is immersed in mixed liquor, then, take out dry, immersing standing time is 30 minutes, the hot-air dry of 40 DEG C, drying time is 1 hour;
F, get the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, each 10 grams of Radix Arnebiae (Radix Lithospermi) Chinese crude drug, add 120 grams of water, soak after 20 minutes, boil 25 minutes, pouring liquids, medicinal residues add water 100 grams again, again boil 20 minutes, merge twice liquid, add water standardize solution 200 milliliters, becomes compound Chinese medicine water cooking liquid; Get the obtained cross-linked alginate complex of E step and immerse compound Chinese medicine water cooking liquid, then take out drying, immersing herbs ' water-decoctum standing time is 2 minutes, and hot blast condition is the hot-air of temperature 40 DEG C, and drying time is 1 hour;
G, get the epoxy natural rubber that epoxidation level is 35%, be dissolved in oxolane, be mixed with 1% solution, then cleaning is coated on not in sticky paper by the tape casting, the hot-air dry of 70 DEG C, drying time is 1 hour, and the thickness of acquisition is the epoxy natural rubber thin film of 0.1 millimeter.
H, compound
The epoxy natural rubber Film laminated that the complex obtain step F and step G obtain, namely obtains the alginate dressing of described treatment decubital ulcer.
Embodiment 3
Treat a preparation method for the alginate medical dressing of decubital ulcer, comprise the steps:
A, to get smashing fineness be the content ratio of 35 object sharkskins and shark cartilage is the mixture dry product of 6:4, and add the 0.1N/L NaOH solution of 1:15 quality, soak 5 hours at 8 DEG C of temperature, taking-up drains; Add the petroleum ether of 1:10, soak 12 hours at 8 DEG C of temperature, drain with after distilled water wash; Add the acetic acid solution of 3.0% of 1:10 again, at 8 DEG C, stir 48 hours under the condition of mixing speed 400rpm, centrifugal under the condition of 40000 turns/min; Get supernatant and add 1mol/L sodium sulfate, through precipitation, filtration, distilled water wash, the acetic acid solution adding 3.0% of 1:10 again dissolves, the molecular cut off adopting Guangzhou Chao Yu membrane separation technique company limited to supply is the ultrafilter membrane ultrafiltration of 100kDa, filtrate lyophilization, obtain sharkskin and cartilage mixing collagen protein, then getting 2% acetic acid solution dissolving, to be made into containing the former protein concentration of epoxy glue be the solution of 0.3%;
B, get solution prepared by step A, under the condition of low whipping speed 400rpm, add sodium alginate 0.8%; Add sodium polyacrylate, the weight ratio of sodium alginate and sodium polyacrylate is 1:0.3; Add pigskin collagen, the weight ratio of sodium alginate and pigskin collagen is 1:0.06, stirs 20 minutes;
C, be solvent with distilled water, get bFGF somatomedin, under the condition of low whipping speed 400rpm, preparation chitosan quaternary ammonium salt and somatomedin composite solution, in solution, chitosan quaternary ammonium salt content is 0.4%, bFGF growth factor content is 0.001%, stirs 10 minutes;
D, the alginate composite solution that obtains in step B are as the outer solution of spinning, the chitosan-containing quaternary ammonium salt obtained in step C and somatomedin composite solution are as the internal layer solution of spinning, carry out coaxial electrostatic spinning, voltage be 30kV, distance between fixed anode and negative electrode is 18cm, temperature is under the condition of 28 ~ 30 DEG C, carry out coaxial electrostatic spinning, dash receiver obtains faint yellow, with the cellosilk of nucleocapsid structure and the alginate fiber film be made up of cellosilk;
E, be solvent with distilled water, adopt calcium chloride and zinc chloride, preparation contains the chloride mixed liquor of 3% calcium chloride and 0.5% zinc chloride, the nano fibrous membrane of D step gained is immersed in mixed liquor, then, take out dry, immersing standing time is 25 minutes, the hot-air dry of 40 DEG C, drying time is 1.5 hours;
F, get the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, each 10 grams of Radix Arnebiae (Radix Lithospermi) Chinese crude drug, add 120 grams of water, soak after 20 minutes, boil 25 minutes, pouring liquids, medicinal residues add water 100 grams again, again boil 20 minutes, merge twice liquid, add water standardize solution 200 milliliters, becomes compound Chinese medicine water cooking liquid; Get the obtained cross-linked alginate complex of E step and immerse compound Chinese medicine water cooking liquid, then take out drying, immersing herbs ' water-decoctum standing time is 2 minutes, and hot blast condition is the hot-air of temperature 37 DEG C, and drying time is 1.5 hours;
G, get the epoxy natural rubber that epoxidation level is 40%, be dissolved in oxolane, be mixed with 1% solution, then cleaning is coated on not in sticky paper by the tape casting, the hot-air dry of 69 DEG C, drying time is 1 hour, and the thickness of acquisition is the epoxy natural rubber thin film of 0.08 millimeter;
H, compound
The epoxy natural rubber Film laminated that the complex obtain step F and step G obtain, namely obtains the alginate dressing of described treatment decubital ulcer.
Embodiment 4
Treat a preparation method for the alginate medical dressing of decubital ulcer, comprise the steps:
A, to get smashing fineness be the content ratio of 40 object sharkskins and shark cartilage is the mixture dry product of 7:3, and add the 0.1N/L NaOH solution of 1:15 quality, soak 5 hours at 8 DEG C of temperature, taking-up drains; Add the petroleum ether of 1:10, soak 12 hours at 8 DEG C of temperature, drain with after distilled water wash; Add the acetic acid solution of 2.8% of 1:10 again, at 8 DEG C, stir 48 hours under the condition of mixing speed 3500rpm, centrifugal under the condition of 40000 turns/min; Get supernatant and add 1mol/L sodium sulfate, through precipitation, filtration, distilled water wash, the acetic acid solution adding 2.8% of 1:10 again dissolves, the molecular cut off adopting Guangzhou Chao Yu membrane separation technique company limited to supply is the ultrafilter membrane ultrafiltration of 100kDa, filtrate lyophilization, obtain sharkskin and cartilage mixing collagen protein, then getting 2% acetic acid solution dissolving, to be made into containing the former protein concentration of epoxy glue be the solution of 0.3%;
B, get solution prepared by step A, under the condition of low whipping speed 350rpm, add sodium alginate 0.6%; Add sodium polyacrylate, the weight ratio of sodium alginate and sodium polyacrylate is 1:0.25; Add pigskin collagen, the weight ratio of sodium alginate and pigskin collagen is 1:0.07, stirs 20 minutes;
C, be solvent with distilled water, get bFGF somatomedin, under the condition of low whipping speed 350rpm, preparation chitosan quaternary ammonium salt and somatomedin composite solution, in solution, chitosan quaternary ammonium salt content is 0.35%, bFGF growth factor content is 0.001%, stirs 10 minutes;
D, the alginate composite solution that obtains in step B are as the outer solution of spinning, the chitosan-containing quaternary ammonium salt obtained in step C and somatomedin composite solution are as the internal layer solution of spinning, carry out coaxial electrostatic spinning, voltage be 30kV, distance between fixed anode and negative electrode is 18cm, temperature is under the condition of 28 ~ 30 DEG C, carry out coaxial electrostatic spinning, dash receiver obtains faint yellow, with the cellosilk of nucleocapsid structure and the alginate fiber film be made up of cellosilk;
E, be solvent with distilled water, adopt calcium chloride and zinc chloride, preparation contains the chloride mixed liquor of 3% calcium chloride and 0.5% zinc chloride, the nano fibrous membrane of D step gained is immersed in mixed liquor, then, take out dry, immersing standing time is 28 minutes, the hot-air dry of 36 DEG C, drying time is 1.2 hours;
F, get the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, each 10 grams of Radix Arnebiae (Radix Lithospermi) Chinese crude drug, add 120 grams of water, soak after 20 minutes, boil 25 minutes, pouring liquids, medicinal residues add water 100 grams again, again boil 20 minutes, merge twice liquid, add water standardize solution 200 milliliters, becomes compound Chinese medicine water cooking liquid; Get the obtained cross-linked alginate complex of E step and immerse compound Chinese medicine water cooking liquid, then take out drying, immersing herbs ' water-decoctum standing time is 2 minutes, and hot blast condition is the hot-air of temperature 36 DEG C, and drying time is 1.5 hours;
G, get the epoxy natural rubber that epoxidation level is 30%, be dissolved in oxolane, be mixed with 1% solution, then cleaning is coated on not in sticky paper by the tape casting, the hot-air dry of 68 DEG C, drying time is 1 hour, and the thickness of acquisition is the epoxy natural rubber thin film of 0.06 millimeter;
H, compound
The epoxy natural rubber Film laminated that the complex obtain step F and step G obtain, namely obtains the alginate dressing of described treatment decubital ulcer.
Embodiment 4
Treat a preparation method for the alginate medical dressing of decubital ulcer, comprise the steps:
A, to get smashing fineness be the content ratio of 40 object sharkskins and shark cartilage is the mixture dry product of 7:3, and add the 0.1N/L NaOH solution of 1:15 quality, soak 5 hours at 8 DEG C of temperature, taking-up drains; Add the petroleum ether of 1:10, soak 12 hours at 8 DEG C of temperature, drain with after distilled water wash; Add the acetic acid solution of 2.8% of 1:10 again, at 8 DEG C, stir 48 hours under the condition of mixing speed 3500rpm, centrifugal under the condition of 40000 turns/min; Get supernatant and add 1mol/L sodium sulfate, through precipitation, filtration, distilled water wash, the acetic acid solution adding 2.8% of 1:10 again dissolves, the molecular cut off adopting Guangzhou Chao Yu membrane separation technique company limited to supply is the ultrafilter membrane ultrafiltration of 100kDa, filtrate lyophilization, obtain sharkskin and cartilage mixing collagen protein, then getting 2% acetic acid solution dissolving, to be made into containing the former protein concentration of epoxy glue be the solution of 0.3%;
B, get solution prepared by step A, under the condition of low whipping speed 350rpm, add sodium alginate 0.6%; Add sodium polyacrylate, the weight ratio of sodium alginate and sodium polyacrylate is 1:0.25; Add pigskin collagen, the weight ratio of sodium alginate and pigskin collagen is 1:0.07, stirs 20 minutes;
C, be solvent with distilled water, get bFGF somatomedin, under the condition of low whipping speed 350rpm, preparation chitosan quaternary ammonium salt and somatomedin composite solution, in solution, chitosan quaternary ammonium salt content is 0.35%, bFGF growth factor content is 0.001%, stirs 10 minutes;
D, the alginate composite solution that obtains in step B are as the outer solution of spinning, the chitosan-containing quaternary ammonium salt obtained in step C and somatomedin composite solution are as the internal layer solution of spinning, carry out coaxial electrostatic spinning, voltage be 30kV, distance between fixed anode and negative electrode is 18cm, temperature is under the condition of 28 ~ 30 DEG C, carry out coaxial electrostatic spinning, dash receiver obtains faint yellow, with the cellosilk of nucleocapsid structure and the alginate fiber film be made up of cellosilk;
E, be solvent with distilled water, adopt calcium chloride and zinc chloride, preparation contains the chloride mixed liquor of 3% calcium chloride and 0.5% zinc chloride, the nano fibrous membrane of D step gained is immersed in mixed liquor, then, take out dry, immersing standing time is 28 minutes, the hot-air dry of 36 DEG C, drying time is 1.2 hours;
F, get the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, each 10 grams of Radix Arnebiae (Radix Lithospermi) Chinese crude drug, add 120 grams of water, soak after 20 minutes, boil 25 minutes, pouring liquids, medicinal residues add water 100 grams again, again boil 20 minutes, merge twice liquid, add water standardize solution 200 milliliters, becomes compound Chinese medicine water cooking liquid; Get the obtained cross-linked alginate complex of E step and immerse compound Chinese medicine water cooking liquid, then take out drying, immersing herbs ' water-decoctum standing time is 2 minutes, and hot blast condition is the hot-air of temperature 36 DEG C, and drying time is 1.5 hours;
G, get the epoxy natural rubber that epoxidation level is 30%, be dissolved in oxolane, be mixed with 1% solution, then cleaning is coated on not in sticky paper by the tape casting, the hot-air dry of 68 DEG C, drying time is 1 hour, and the thickness of acquisition is the epoxy natural rubber thin film of 0.06 millimeter;
H, compound
The epoxy natural rubber Film laminated that the complex obtain step F and step G obtain, namely obtains the alginate dressing of described treatment decubital ulcer.
Claims (10)
1. treat an alginate medical dressing for decubital ulcer, it is characterized in that: described dressing has two-layer composite, be namely made up of bottom and surface layer, bottom is alginate composite film, and surface layer is natural rubber rete.
2. the alginate medical dressing for the treatment of decubital ulcer according to claim 1, is characterized in that: described natural rubber rete is the epoxy natural rubber material film that epoxidation level reaches 30 ~ 40%, and the thickness of this film is 0.04 ~ 0.1 millimeter; Described alginate composite film is the fibrous membrane be made up of sodium alginate, sodium polyacrylate, shark collagen protein, pigskin collagen, chitosan quaternary ammonium salt, basic fibroblast growth factor (bFGF) different materials, and the thickness of this film is 1 ~ 5 millimeter; Described fibrous membrane is arranged under the overlay by cellosilk to form, and this cellosilk has core, shell structure, and wherein shell is made up of sodium alginate, sodium polyacrylate, shark collagen protein, pigskin collagen material; Sandwich layer is made up of chitosan quaternary ammonium salt and somatomedin material, the material layer of the extract of the bletilla that fibrous membrane surface-coated has the material layer of chloride containing calcium and zinc chloride and process to concoct, Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, Radix Arnebiae (Radix Lithospermi).
3. the preparation method of the alginate medical dressing for the treatment of decubital ulcer as claimed in claim 1 or 2, is characterized in that comprising the steps:
A, preparation shark collagen solution
Get the mixture dry product of sharkskin and bone, add a certain amount of NaOH solution after pulverizing to soak, drain, after adding petroleum ether immersion, drain, add again acetic acid solution extract, centrifugal after, get supernatant and add metabisulfite solution precipitation, precipitate, through distilled water wash, lyophilization, obtains sharkskin and bone mixing collagen protein, be dissolved in certain density acetic acid solution, obtained shark collagen solution.
B, prepare the outer solution of coaxial electrostatic spinning
Get the solution that steps A obtains, add sodium alginate, sodium polyacrylate, pigskin collagen, be uniformly mixed, obtain a kind of composite solution containing alginate mixture;
C, prepare coaxial electrostatic spinning sandwich layer solution
Chitosan quaternary ammonium salt is dissolved in distilled water, adds basic fibroblast growth factor (bFGF), stir, obtain a kind of composite solution containing chitosan quaternary ammonium salt and somatomedin;
D, spinning
Adopt coaxial electrostatic spinning method, the alginate composite solution allowing step B obtain is as the outer solution of coaxial electrostatic spinning, the internal layer solution of composite solution as coaxial electrostatic spinning containing chitosan quaternary ammonium salt and somatomedin allowing step C obtain, carry out coaxial spinning, obtain based on alginate with the double-deck cellosilk of nucleocapsid and the fibrous membrane that is made up of cellosilk;
E, crosslinked
The fibrous membrane that obtained by step D immerses the some time in chloride mixture solution, after taking-up under hot blast condition the dry some time, obtain cross-linked alginate complex;
F, dipping
Cross-linked alginate complex step e obtained immerses in the compound Chinese medicine water cooking liquid containing the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, Radix Arnebiae (Radix Lithospermi) composition places the some time, take out dry certain hour under hot blast condition, obtain the cross-linked alginate complex containing Chinese medicine ingredients;
G, prepare epoxy natural rubber thin film
The tape casting is adopted to prepare the epoxy natural rubber thin film that thickness is 0.04 ~ 0.1 millimeter;
H, compound
The epoxy natural rubber Film laminated that the complex obtain step F and step G obtain, namely obtains the alginate dressing of described treatment decubital ulcer.
4. preparation method according to claim 3, is characterized in that: in steps A, the mixture dry product of sharkskin and bone is that the ratio of sharkskin and shark cartilage is respectively 6 ~ 7:4 ~ 3 mixture, through being dried to water content lower than 5% dry product; This dry product is pulverized as after fineness 20-50 order, adds the 0.1N/L NaOH solution of 1:15 quality, and soak 5 hours at 8 DEG C of temperature, taking-up drains; Add the petroleum ether of 1:10, soak 12 hours at 8 DEG C of temperature, drain with after distilled water wash; Add the acetic acid solution of the 2.5%-3.5% of 1:10 again and at 8 DEG C, mixing speed is that 300 ~ 500rpm stirs 48 hours; Centrifugal through 40000 turns/min, get supernatant and add 1mol/L sodium sulfate, through precipitation, filtration, distilled water wash, the acetic acid solution adding the 2.5%-3.5% of 1:10 again dissolves, adopt molecular cut off to be the ultrafiltration of 100kDa ultrafilter membrane, filtrate lyophilization, obtain sharkskin and cartilage mixing collagen protein, get 2% acetic acid solution again to dissolve, being made into containing the former protein concentration of epoxy glue is the solution of 0.3%.
5. preparation method according to claim 3, it is characterized in that: the sodium alginate addition described in step B is 0.4 ~ 0.8%, the weight ratio of sodium alginate and sodium polyacrylate is 1:0.2 ~ 0.4, the weight ratio of sodium alginate and pigskin collagen is 1:0.05 ~ 0.08, this pigskin collagen is the low molecule collagen protein of molecular weight at 10D to 30D, mixing speed is 300 ~ 500rpm, stirs 20 minutes.
6. preparation method according to claim 3, is characterized in that: the chitosan quaternary ammonium salt in the chitosan quaternary ammonium salt described in step C and somatomedin composite solution is the quaternised modified product of chitosan, and content is 0.3 ~ 0.5%; Basic fibroblast growth factor (bFGF) content is 0.001%, and mixing speed is 300 ~ 500rpm, stirs 10 minutes.
7. preparation method according to claim 3, it is characterized in that the coaxial electrostatic spinning method described in step D, the alginate composite solution that obtains in the step B outer solution as spinning, the chitosan-containing quaternary ammonium salt obtained in step C and somatomedin composite solution are as the internal layer solution of spinning, be 30kV at voltage, distance between fixed anode and negative electrode is 18cm, temperature is under the condition of 28 ~ 30 DEG C, carry out coaxial electrostatic spinning, dash receiver obtain faint yellow, with the cellosilk of nucleocapsid structure and the alginate fiber film be made up of cellosilk.
8. preparation method according to claim 3, it is characterized in that the chloride mix solution described in step e is the mixed aqueous solution containing 3% calcium chloride and 0.5% zinc chloride, after immersing, standing time is 20 to 30 minutes, then take out, dry in 35 ~ 40 DEG C of hot-airs, drying time is 1 ~ 2 hour.
9. preparation method according to claim 3, it is characterized in that: the water cooking liquid of compound Chinese medicine described in step F, be bletilla, Radix Paeoniae Rubra, Sanguis Draxonis, Semen Momordicae, each 10 grams of Radix Arnebiae (Radix Lithospermi), add 120 grams of water, soak after 20 minutes, boil 25 minutes, pouring liquids, medicinal residues add water 100 grams again, again boil 20 minutes, merge twice liquid, add water standardize solution 200 milliliters, becomes compound Chinese medicine water cooking liquid; After cross-linked alginate complex immersion herbs ' water-decoctum, standing time is 2 minutes, then takes out drying, and dry in 35 ~ 40 DEG C of hot-airs, drying time is 1 ~ 2 hour.
10. preparation method according to claim 3, it is characterized in that: the epoxy natural rubber thin film described in step G to be epoxidation level be 30 ~ 40% epoxy natural rubber, be dissolved in oxolane, be mixed with 1% solution, then cleaning is coated on not in sticky paper by the tape casting, through the hot-air dry of 68 ~ 70 DEG C, drying time is 40 minutes, and the thickness of acquisition is the epoxy natural rubber thin film of 0.04 ~ 0.1 millimeter.
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| CN111330063A (en) * | 2020-01-21 | 2020-06-26 | 广东工业大学 | Nanofiber membrane and preparation method thereof |
| CN115531594A (en) * | 2022-10-19 | 2022-12-30 | 南通大学 | Diabetic wound healing dressing and preparation method thereof |
| CN115531594B (en) * | 2022-10-19 | 2023-07-04 | 南通大学 | Wound healing dressing for diabetes and preparation method thereof |
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