CN104604694A - Seedling culture and transplanting method of improved Ottelia acuminata tissue culture seedling root system - Google Patents
Seedling culture and transplanting method of improved Ottelia acuminata tissue culture seedling root system Download PDFInfo
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- CN104604694A CN104604694A CN201510081606.7A CN201510081606A CN104604694A CN 104604694 A CN104604694 A CN 104604694A CN 201510081606 A CN201510081606 A CN 201510081606A CN 104604694 A CN104604694 A CN 104604694A
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Abstract
The invention discloses a seedling culture and transplanting method of an improved Ottelia acuminata tissue culture seedling root system. The method comprises the three steps of the rooting culture of tissue culture seedlings, the improvement of the Ottelia acuminata tissue culture seedling root system and the heeling-in and transplanting of the Ottelia acuminata tissue culture seedlings. The method has the beneficial effects that: 1. the rooting time of the Ottelia acuminata tissue culture seedlings can be shortened, the tissue culture seedlings are healthy, the root system is developed, the flexibility of roots is good, and the penetrating power of root tips is strong, so that the Ottelia acuminata tissue culture seedling root system can be tightly combined with a non-woven bag and a medium thereof and is directly put into lakes and rivers in which the Ottelia acuminata needs to be planted, and thus the transplanting survival rate of the tissue culture seedlings is greatly improved; 2. the operation is simple, the production cost is low, the seedlings can be cultured and transplanted on a large scale, a technical support is provided for promoting and applying the Ottelia acuminata tissue culture seedlings, and a foundation is laid for the sustainable utilization of the Ottelia acuminata and the protection of ecological environment diversity.
Description
Technical field
The present invention relates to technical field of plant asexual propagation, specifically a kind of seedling fostering and transplanting method improveing edible seaweed flower plantlet in vitro root system.
Background technology
Edible seaweed flower [Otteliaacuminata (Gagnep.) Dandy] has another name called edible seaweed, dragon's paw dish, belongs to the perennial water plants of Hydrocharctaceae.Edible seaweed flower is China's endemic species, is mainly distributed in Yunnan, Guizhou, Guangxi and some areas, Hainan.Edible seaweed flower can grow in lake, pond, irrigation canals and ditches and deep paddy field, requires that water body is clean, as clear as crystal, likes warm.In general 5 to October of florescence, there is flower in warm area the whole year.
Edible seaweed flower be integrate view and admire, eat, the submerged plant of medicinal and slight water pollution purification.Edible seaweed flower the white lobe of yellow stamen spend in full bloom time bubble through the water column, lake surface, river are decorateed flowers blooming like a piece of brocade, there is very high ornamental value.Its leaf and flower amino acid content enrich, and essential amino acid and dispensable amino acid ratio (EAA/NEAA) are 0.718, meet the outstanding protein sources that FAO/WHO recommends.Its delicious refreshing sweet perfume (or spice), often edible also have significantly fat-reducing and cosmetic result.Simultaneously edible seaweed flower or a kind of Chinese traditional herbs material, all herbal medicine, can control difficult urination, constipation, heat are coughed, spit blood, the various diseases such as asthma, stranguria and oedema.In addition, modern study shows, and edible seaweed flower also has algal control water purification function.
In recent years, edible seaweed flower because it is nutritious, high in ornamental value and tool algal control water purification function and receive much concern, demand constantly increases, and the market price goes up year by year.Under the ordering about of interests, people gather edible seaweed flower with carrying out predation formula, make edible seaweed spend wild natural resources distribution area day by reducing, endangered, are now listed in national III level protective plant.
Between the field demand and supply of edible seaweed flower market, contradiction is becoming increasingly acute, and carrying out that the edible seaweed seeds of flowering plants plants is solve the effective way of this contradiction.But edible seaweed flower has a very limited distribution, grain weight is few, and natural propagation is slow, and wild resource amount is few.And adopt method for plant tissue culture can Fast-propagation is a large amount of in a short time edible seaweed seeds of flowering plants seedling, because Plant Tissue Breeding belongs to vegetative propagation, the merit of former stock can be kept, but also there is the high advantage of reproduction coefficient.Edible seaweed flower is submerged plant, and during the plantlet in vitro plantation of edible seaweed flower, root system must fully contact ability smooth growth with water bottom sediment.Therefore, edible seaweed flower plantlet in vitro is cultivated, if plantlet in vitro root system can penetrate non-woven bag in the non-woven bag that the matrix such as sandy soil are housed, became one with matrix, so just can throw kind of an edible seaweed as rice transplanting paddy rice to have spent, and non-woven bag can natural decomposition, can not pollute natural environment.But the root system of edible seaweed flower plantlet in vitro is more fragile, cannot penetrate non-woven fabrics Seedling bag, seriously hinder applying of edible seaweed flower plantlet in vitro.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of seedling fostering and the transplanting method of improveing edible seaweed flower plantlet in vitro root system are provided, stage that edible seaweed flower plantlet in vitro is taken root with hardening, heel in, transplant and combine, root system is induced in the plantlet in vitro stage of taking root, take out with regard to hardening when root system children is tender, with root-growing agent process root, make the tough and tensile prosperity of edible seaweed flower plantlet in vitro root system, penetrate non-woven bag, became one with non-woven bag and sandy soil wherein, be convenient to edible seaweed flower plantlet in vitro sink under water, smooth growth.The method is simple to operate, production cost is low, application is strong, stability and high efficiency.
The technical scheme that the present invention solves the problems of the technologies described above is:
Improve seedling fostering and the transplanting method of edible seaweed flower plantlet in vitro root system, operating procedure is as follows:
1. plantlet in vitro culture of rootage
On superclean bench, the aseptic bud that the 3 ~ 7cm obtained by edible seaweed flower Multiplying culture is high, be cut into simple bud, in access root media, cultivate 15 ~ 30d, root length is about 0.5cm, obtains edible seaweed flower plantlet in vitro; Described root media is: MS+KT 0.05 ~ 0.5mg/L+6-BA 0.05 ~ 0.5mg/L+NAA0.1 ~ 3.0mg/L+ active carbon 1.0g/L+ sucrose 20g/L+ agar 5g/L, and pH value is 5.8; The condition of culture in culture of rootage stage is: cultivation temperature is 25 ± 3 DEG C, and light application time is 12h/d, and intensity of illumination is 1500lx.
2. edible seaweed flower plantlet in vitro root system improvement
The edible seaweed flower plantlet in vitro taking out step 1 shifts out culturing room, and indoor hardening 3d, gets seedling, and cleaned by the medium that root adheres to carefully, dry in the air and be put in 20min in net dish, root is soaked in 60min in root-growing agent; Described root-growing agent is: NAA 50.0 ~ 1000.0mg/L+IBA100.0 ~ 1000.0mg/L+6-BA20.0 ~ 200.0mg/L+Vc1.0 ~ 10.0mg/L, and pH value is 5.5.
3. heeling in and transplanting of plantlet in vitro spent by edible seaweed
By in step 2 edible seaweed flower plantlet in vitro heel in bore be 5cm non-woven bag heel in matrix, be nursery in 5cm paddy field at water depth, 50d, coolant-temperature gage is normal temperature, and transplanting survival rate is 68.6% ~ 100%; Described matrix of heeling in is: 1/3 sand+2/3 yellow mud.
Above-mentioned root-growing agent is preferably: NAA200mg/L+IBA500mg/L+6-BA100mg/L+Vc5.0mg/L, pH value is 5.5.
In above-mentioned root-growing agent, NAA is a-methyl α-naphthyl acetate, and IBA is indolebutyric acid, and 6-BA is 6-benzyl aminopurine, and Vc is vitamin c, and it is pure that reagent is analysis, and water is ultra-pure water.
Above-mentionedly take root in the stage, KT is kinetin, and reagent is pure for analyzing, and water is ultra-pure water.
Advantage of the present invention:
1. adopt the inventive method can shorten the rootage duration of edible seaweed flower plantlet in vitro, plantlet in vitro is healthy and strong, well developed root system, the pliability of root is good, the penetration power of the tip of a root is strong, edible seaweed flower plantlet in vitro root system can be made to be combined closely with non-woven bag and matrix thereof, to be directly invested in and requiredly to plant in the lake and rivers that edible seaweed spends, raising plantlet in vitro transplanting survival rate greatly.
2. the inventive method is simple to operate, and production cost is low, can cultivate on a large scale and transplant seedling, and applying for edible seaweed flower plantlet in vitro provides technical support, for edible seaweed flower sustainable use pattern ecotope diversity lays the foundation.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
Improve seedling fostering and the transplanting method of edible seaweed flower plantlet in vitro root system, comprise the following steps:
1. on superclean bench, the aseptic bud that the 3 ~ 7cm obtained by edible seaweed flower Multiplying culture is high, be cut into simple bud, in access root media, in culturing room, cultivate 30d, root length is about 0.5cm, obtains edible seaweed flower plantlet in vitro; Described root media is: MS+KT0.05mg/L+6-BA0.05mg/L+NAA0.1mg/L+ active carbon 1.0g/L+ sucrose 20g/L+ agar 5g/L, and pH value is 5.8; The condition of culture of described culturing room is: cultivation temperature is 25 ± 3 DEG C, and light application time is 12h/d, and intensity of illumination is 1500lx.
2. the edible seaweed flower plantlet in vitro taking out step 1 shifts out culturing room, and indoor hardening 3d, gets seedling, and cleaned by the medium that root adheres to carefully, dry in the air and be put in 20min in net dish, root is soaked in 60min in root-growing agent.Described root-growing agent is: NAA50mg/L+IBA100mg/L+6-BA20mg/L+Vc1.0mg/L, and pH value is 5.5.
3. by step 2 edible seaweed flower plantlet in vitro heel in bore be 5cm non-woven bag heel in matrix, be nursery 50d in 5cm paddy field at water depth, coolant-temperature gage is normal temperature, and edible seaweed flower plantlet in vitro root system is more flourishing, seedling growing way is better, but tip of a root penetration power is inadequate, and part seedling does not penetrate non-woven bag, when being transplanted in lake, seedling is separated with non-woven bag and matrix thereof, float on the water surface, cannot survive, survival rate is 68.6%; Described matrix of heeling in is: 1/3 sand+2/3 yellow mud.
Embodiment 2:
Improve seedling fostering and the transplanting method of edible seaweed flower plantlet in vitro root system, comprise the following steps:
1., on superclean bench, the aseptic bud that the 3 ~ 7cm obtained by edible seaweed flower Multiplying culture is high, be cut into simple bud, in access root media, in culturing room, cultivate 20d, root length is about 0.5cm, obtains edible seaweed flower plantlet in vitro.Described root media is: MS+KT0.5mg/L+6-BA0.5mg/L+NAA3.0mg/L+ active carbon 1.0g/L+ sucrose 20g/L+ agar 5g/L, and pH value is 5.8; The condition of culture of described culturing room is: cultivation temperature is 25 ± 3 DEG C, and light application time is 12h/d, and intensity of illumination is 1500lx.
2. the edible seaweed flower plantlet in vitro taking out step 1 shifts out culturing room, and indoor hardening 3d, gets seedling, and cleaned by the medium that root adheres to carefully, dry in the air and be put in 20min in net dish, root is soaked in 60min in root-growing agent.Described root-growing agent is: NAA1000mg/L+IBA1000mg/L+6-BA200mg/L+Vc10.0mg/L, and pH value is 5.5;
3. by step 2 edible seaweed flower plantlet in vitro heel in bore be 5cm non-woven bag heel in matrix, be nursery 50d in 5cm paddy field at water depth, coolant-temperature gage is normal temperature, edible seaweed flower plantlet in vitro root system is not very flourishing, the growing way of seedling is slightly weak, but the penetration power of the tip of a root is strong, and transplanting survival rate is 80.7%; Described matrix of heeling in is: 1/3 sand+2/3 yellow mud.
Embodiment 3:
Improve seedling fostering and the transplanting method of edible seaweed flower plantlet in vitro root system, comprise the following steps:
1., on superclean bench, the aseptic bud that the 3 ~ 7cm obtained by edible seaweed flower Multiplying culture is high, be cut into simple bud, in access root media, in culturing room, cultivate 15d, root length is about 0.5cm, obtains edible seaweed flower plantlet in vitro.Described root media is: MS+KT0.1mg/L+6-BA0.1mg/L+NAA1.0mg/L+ active carbon 1.0g/L+ sucrose 20g/L+ agar 5g/L, and pH value is 5.8; The condition of culture of described culturing room is: cultivation temperature is 25 ± 3 DEG C, and light application time is 12h/d, and intensity of illumination is 1500lx.
2. the edible seaweed flower plantlet in vitro taking out step 1 shifts out culturing room, and indoor hardening 3d, gets seedling, and cleaned by the medium that root adheres to carefully, dry in the air and be put in 20min in net dish, root is soaked in 60min in root-growing agent.Described root-growing agent is: NAA 200mg/L+IBA500mg/L+6-BA100mg/L+Vc5.0mg/L, and pH value is 5.5;
3. by step 2 edible seaweed flower plantlet in vitro heel in bore be 5cm non-woven bag heel in matrix, be nursery 50d in 5cm paddy field at water depth, coolant-temperature gage is normal temperature, edible seaweed flower plantlet in vitro well developed root system, penetration power is strong, and seedling is healthy and strong, growing way is good, and transplanting survival rate is 100%; Described matrix of heeling in is: 1/3 sand+2/3 yellow mud.
Claims (3)
1. improve seedling fostering and the transplanting method of edible seaweed flower plantlet in vitro root system, it is characterized in that, operating procedure is as follows:
1) plantlet in vitro culture of rootage
On superclean bench, the aseptic bud that the 3 ~ 7cm obtained by edible seaweed flower Multiplying culture is high, be cut into simple bud, in access root media, cultivate 15 ~ 30d, root length is 0.5cm, obtains edible seaweed flower plantlet in vitro; Described root media is: MS+KT 0.05 ~ 0.5mg/L+6-BA 0.05 ~ 0.5mg/L+NAA0.1 ~ 3.0mg/L+ active carbon 1.0g/L+ sucrose 20g/L+ agar 5g/L, and pH value is 5.8; The condition of culture in culture of rootage stage is: cultivation temperature is 25 ± 3 DEG C, and light application time is 12h/d, and intensity of illumination is 1500lx;
2) edible seaweed flower plantlet in vitro root system improvement
Take out step 1) edible seaweed flower plantlet in vitro shift out culturing room, indoor hardening 3d, gets seedling, carefully by root adhere to medium clean, dry in the air and be put in 20min in net dish, root is soaked in 60min in root-growing agent; Described root-growing agent is: NAA 50.0 ~ 1000.0mg/L+IBA100.0 ~ 1000.0mg/L+6-BA20.0 ~ 200.0mg/L+Vc1.0 ~ 10.0mg/L, and pH value is 5.5;
3) heeling in and transplanting of plantlet in vitro spent by edible seaweed
By step 2) in edible seaweed flower plantlet in vitro heel in bore be 5cm non-woven bag heel in matrix, be nursery in 5cm paddy field at water depth, 50d, coolant-temperature gage is normal temperature, and transplanting survival rate is 68.6% ~ 100%; Described matrix of heeling in is: 1/3 sand+2/3 yellow mud.
2. a kind of seedling fostering and transplanting method improveing edible seaweed flower plantlet in vitro root system according to claim 1, it is characterized in that: described root-growing agent is: NAA200mg/L+IBA500mg/L+6-BA100mg/L+Vc5.0mg/L, pH value is 5.5.
3. a kind of seedling fostering and transplanting method improveing edible seaweed flower plantlet in vitro root system according to claim 1, is characterized in that, in described root-growing agent, NAA is a-methyl α-naphthyl acetate, and IBA is indolebutyric acid, and 6-BA is 6-benzyl aminopurine, Vc is vitamin c, and be analysis pure, water is ultra-pure water.Above-mentionedly take root in the stage, KT is kinetin, and reagent is pure for analyzing, and water is ultra-pure water.
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Cited By (7)
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| CN105393908A (en) * | 2015-11-16 | 2016-03-16 | 水生藻安生物科技（武汉）有限公司 | Rapid modularization planting method for submerged plant tissue culture seedlings and modularization combination |
| CN107258547A (en) * | 2017-08-14 | 2017-10-20 | 天津科润农业科技股份有限公司 | A kind of method of cauliflower Multiple Buds regeneration plant and application |
| CN109315264A (en) * | 2018-11-09 | 2019-02-12 | 江苏江达生态科技有限公司 | A kind of formula and preparation method of submerged plant planting mud ball matrix |
| CN109699443A (en) * | 2019-02-25 | 2019-05-03 | 重庆大学 | A kind of submerged plant planting matrix and the method using the matrix kind planting submerged plant |
| CN112136524A (en) * | 2019-06-27 | 2020-12-29 | 贵州思源农旅综合开发有限公司 | Cuttage method for rhododendron variety Gemiria |
| WO2021248714A1 (en) * | 2020-06-11 | 2021-12-16 | 南大（常熟）研究院有限公司 | Virus-free seedling production method suitable for fast propagation of submersed plants |
| CN115812533A (en) * | 2022-11-21 | 2023-03-21 | 中国科学院武汉植物园 | Method for rapidly recovering bitter thorn population |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105393908A (en) * | 2015-11-16 | 2016-03-16 | 水生藻安生物科技（武汉）有限公司 | Rapid modularization planting method for submerged plant tissue culture seedlings and modularization combination |
| CN105393908B (en) * | 2015-11-16 | 2018-07-31 | 水生藻安生物科技（武汉）有限公司 | The method and modular combination of the quick modularization plantation of submerged plant tissue-cultured seedling |
| CN107258547A (en) * | 2017-08-14 | 2017-10-20 | 天津科润农业科技股份有限公司 | A kind of method of cauliflower Multiple Buds regeneration plant and application |
| CN109315264A (en) * | 2018-11-09 | 2019-02-12 | 江苏江达生态科技有限公司 | A kind of formula and preparation method of submerged plant planting mud ball matrix |
| CN109699443A (en) * | 2019-02-25 | 2019-05-03 | 重庆大学 | A kind of submerged plant planting matrix and the method using the matrix kind planting submerged plant |
| CN112136524A (en) * | 2019-06-27 | 2020-12-29 | 贵州思源农旅综合开发有限公司 | Cuttage method for rhododendron variety Gemiria |
| WO2021248714A1 (en) * | 2020-06-11 | 2021-12-16 | 南大（常熟）研究院有限公司 | Virus-free seedling production method suitable for fast propagation of submersed plants |
| CN115812533A (en) * | 2022-11-21 | 2023-03-21 | 中国科学院武汉植物园 | Method for rapidly recovering bitter thorn population |
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