CN104472186A - Liquid inoculation method for artificially planting cistanche - Google Patents

Liquid inoculation method for artificially planting cistanche Download PDF

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Publication number
CN104472186A
CN104472186A CN201410749542.9A CN201410749542A CN104472186A CN 104472186 A CN104472186 A CN 104472186A CN 201410749542 A CN201410749542 A CN 201410749542A CN 104472186 A CN104472186 A CN 104472186A
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inoculation
liquid
seed
cistanche
ditch
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CN104472186B (en
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刘永萍
鲁天平
刘爱民
郭靖
宁虎森
杨瑞
伊丽米努尔
莫双铭
武秀红
江晓珩
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INSTITUTE OF AFFORESTATION AND DESERTIFICATION XINJIANG ACADEMY OF FOREST
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INSTITUTE OF AFFORESTATION AND DESERTIFICATION XINJIANG ACADEMY OF FOREST
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
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  • Pretreatment Of Seeds And Plants (AREA)
  • Catching Or Destruction (AREA)

Abstract

The invention discloses a liquid inoculation method for artificially planting cistanche. The liquid inoculation method includes the following steps of cistanche seed grading, screening and germinating treatment, modifying of an inoculating device, inoculating liquid preparing, inoculating ditch excavating, farmyard manure fertilizing, liquid inoculating, inoculating ditch backfilling and conventional irrigation management after watering for the first time and soil moisture filling. The liquid inoculation method has the advantages that by the liquid inoculation method for cistanche seeds, germination, growing and restoring of host root systems can be promoted, distance between the seeds and the host root systems can be shortened, inoculating section can be expanded to increase contact probability of the seeds and host roots, and inoculating success rate and yield are increased while seed consumption is reduced. Experiments show that compared with methods of direct broadcast sowing and seed paper inoculating, the liquid inoculation method is seed saving, convenient for operation, short in inoculation period and highest in inoculation rate, spray gun equipment with diameter of 2.0mm and used in the method is medium in seed spraying quantity and good in spraying effect, seed consumption is reduced due to spraying, and the liquid inoculation method is high in inoculation rate, convenient for operation during production and easy for popularization.

Description

Artificial planting saline cistanche liquid inoculation method
Technical field
The present invention relates to biotechnology and field of agricultural cultivation, especially relate to the liquid inoculation method of cistanche salsa artificial plantation.
Background technology
Domestic scholars Liu Ming front yard, Li Tianran etc. are reported on Chinese tamarisk after the success of artificial infection Cistanche tubulosa, and inoculation technique is constantly updated, and as used inoculating paper inoculation, inoculation ditch and the inoculation of induction ditch etc. are dug in field, but Field inoculation rate is still lower.Root pipe inoculation technique, can make the rate of vaccination of Cistanche tubulosa reach 92.15%, and the inoculation band (inoculating paper technology) be derived at present, seed slurry is stained with root and inoculation dish (nutrition revulsion), but because rate of vaccination is low, or production operation is complicated, or operability is poor, is not suitable for large scale application.
Summary of the invention
Object of the present invention is exactly for above-mentioned defect of the prior art, provides artificial planting saline cistanche liquid inoculation method.
Liquid inoculation technology its object is to, through injection apparatus, cistanche deserticola seed is sprayed on host's root system section by uniform liquid, promotes the sprouting of host's root system, growth, recovery; Shorten seed and host's root system distance, to increase the contact probability of seed and host's root system, increase inoculating surfaces, increase success ratio of inoculation, minimizing sowing quantity, aborning handled easily, be easy to popularization.
To achieve these goals, technical scheme provided by the invention is: artificial planting saline cistanche liquid inoculation method, comprises the following steps:
1) cistanche deserticola seed sizing screening and seed germination process;
2) transformation of classification inoculation apparatus;
3) preparation of inoculation liquid;
4) excavation inoculation ditch;
5) farmyard manure is executed;
6) liquid inoculation is carried out with inoculating facility;
7) backfill inoculation ditch;
8) after water head hydration moisture in the soil, irrigation management routinely.
Further, above-mentioned artificial planting saline cistanche liquid inoculation method, in described step 1), cistanche deserticola seed sizing screening and seed germination process, concrete steps are as follows: cistanche deserticola seed is divided into three kinds, be respectively Desert Herba Cistanches, Cistanche tubulosa and halophilous herbage, high-quality cistanche deserticola seed after the screening of described particle diameter is respectively diameter and is more than or equal to the halophilous herbage high quality seed that Desert Herba Cistanches high quality seed O.7mm or Cistanche tubulosa high quality seed and diameter are 0.55-O.7 mm, cistanche deserticola seed is carried out grain size grading screening, the high-quality cistanche deserticola seed obtained is sprouted after process through seed-soaking liquid, for subsequent use after drying in the shade.
Further, above-mentioned artificial planting saline cistanche liquid inoculation method, described step 2) in, the transformation of classification inoculation apparatus, concrete steps are as follows: classification inoculation apparatus selects the spray gun 1 of nozzle diameter 1.5-2.0mm, and spray gun inner catheter diameter is more than or equal to 10mm, and extracts soft suction hose;
Further, above-mentioned artificial planting saline cistanche liquid inoculation method, the preparation of inoculation liquid in described step 3), concrete steps are as follows: compound concentration is 2 of 0.02mg/l, the humic acid film of 6-dimethoxy 1,4-benzoquinone, mass concentration 30g/l, the ABT rooting powder solution of mass concentration 50mg/L, obtain inoculation liquid after equal-volume mixing for subsequent use.
Further, above-mentioned artificial planting saline cistanche liquid inoculation method, excavation inoculation ditch in described step 4), concrete steps are as follows: apart from selected host plant 60cm place, excavate the inoculation ditch of dark 70-80cm, ditch with mini-excavator or ditching machine.
Further, above-mentioned artificial planting saline cistanche liquid inoculation method, execute farmyard manure in described step 5), concrete steps are as follows: in inoculation ditch, apply the fertilizer that becomes thoroughly decomposed on a small quantity, and seed is isolated with fertile, executes farmyard manure 500g/m in ditch.
Further, above-mentioned artificial planting saline cistanche liquid inoculation method, carry out liquid inoculation with inoculating facility in described step 6), concrete steps are as follows:
A) selecting step 1) put into step 3) inoculation nutrient solution through the high-quality cistanche deserticola seed sprouting process, 1g seed puts into 15kg inoculation liquid;
B) step a) seed inoculation liquid is loaded step 2) liquid inoculation equipment, prepare inoculation;
C) liquid and seed to be sprayed near host plant root system section side in plantation ditch with step b) liquid inoculation equipment, spray is connected on from the section of bottom of trench upwards 30-40cm, and every meter controls ejection inoculation liquid 200-300ml, ejection seed 100-200 grain.
Further, above-mentioned artificial planting saline cistanche liquid inoculation method, backfill inoculation ditch in described step 7), concrete steps are as follows: the inoculation ditch inoculated is immediately with machinery or the appropriate soil of artificial backfill, and backfill soil is treaded, and backfill soil is to hole along 10 cm places.
Further, above-mentioned artificial planting saline cistanche liquid inoculation method, in described step 8) after water head hydration moisture in the soil, irrigation management routinely, concrete steps are as follows: after soil has backfilled, after water head hydration moisture in the soil, irrigation management routinely, keeps soil moisture content at 20%-40%.
Beneficial effect of the present invention is: the liquid inoculation method of cistanche deserticola seed provided by the invention, the sprouting of host's root system, growth, recovery can be promoted, shorten the distance of seed and host's root system, expand inoculation section face, to increase the contact probability of seed and host's root, improve success ratio of inoculation, output, minimizing sowing quantity, artificial cistanche inoculation method has technological innovation.Empirical tests, compare with seed paper inoculation method with directly broadcasting sowing, liquid inoculation method province plants, easy to operate, transfer period is short, rate of vaccination is the highest, the nozzle diameter used in liquid inoculation technology is at the lance driving device of 2.0mm, and spray kind of amount is moderate, and jeting effect is better, spray and reduce sowing quantity, rate of vaccination is high, aborning handled easily, is easy to promote.
Embodiment
embodiment 1:
One, test material:
1, cistanche deserticola seed process:
Three kinds of cistanche deserticola seeds are carried out grain size grading screening, and choose diameter respectively and be greater than Desert Herba Cistanches O.7mm and Cistanche tubulosa high quality seed, diameter is that the halophilous herbage high quality seed of 0.55-O.7 mm is for subsequent use.
2, medicament and instrument:
Pure water, watering can, spray gun (water gun, number of patent application: 201020504380.X), nozzle, NongFuShanQuan mineral water bottle, A3 printing paper.
Two, test method:
1, the selection of classification inoculation apparatus:
Select 1, the watering can of nozzle diameter 1.5-2.0mm, nozzle diameter 1.5-2.0mm spray gun 1, spray gun inner catheter more than diameter 10mm, extract soft suction hose; Cistanche deserticola seed 1000 and 550mL water are loaded classification inoculation apparatus to jetting liquid on blank sheet of paper, calculates the grain number of ejection seed, repeat spray 3 times;
2, spray tip different-diameter spray kind of gauge is calculated:
Select diameter 1.5mm, the nozzle of 2.0mm, 2.5mm, 3.0mm, puts into classification inoculation apparatus to jetting liquid on blank sheet of paper, liquid capacity 550mL by cistanche deserticola seed and water, loads grain weight 1000, calculates the grain number of ejection seed, repeats spray 3 times;
Three, results and analysis:
1, the spray kind situation of classification inoculation apparatus compares:
The spray kind amount comparing result of different vaccination device is as shown in table 1.
Table 1
As can be seen from Table 1, when watering can is equipped in first time jetting liquid, liquid direct projection sprays, and seed has entered into water suction conduit, be not ejected, during second time jetting liquid, liquid direct projection sprays, and has only sprayed 12, enter more seed in water suction conduit, average ejection 7.3 is not very desirable; During spray gun equipment first time jetting liquid, the vaporific ejection of liquid water, spray 60 seeds, on average sprayed 59 seeds for 3 times, spray satisfactory for result, but spray volume is more stable.
2, spray tip different-diameter spray kind of gauge calculation and Analysis:
The spray kind amount comparing result of different spray nozzles diameter is as shown in table 2.
Table 2
As can be seen from Table 2, nozzle diameter is when 2.0mm, and spray kind of amount is moderate, and jeting effect is better, meets the requirement of liquid inoculation, saves sowing quantity.
embodiment 2:
One, test material:
1, cistanche deserticola seed process:
Within 2011, to gather in Jimusar County Desert Herba Cistanches seed (Cistanche deserticola Ma), seed is carried out grain size grading screening, chooses the Desert Herba Cistanches high quality seed that diameter is greater than 0.7mm respectively; Cistanche deserticola seed sprouts process through seed-soaking liquid, dries in the shade for subsequent use.
2, medicament and instrument:
2, (abundant Rake bio tech ltd, Xinjiang buys 6-dimethoxy 1,4-benzoquinone (DMBQ), article number: D149705), humic acid film (Changzhou Yongfeng biologos element factory produce) and ABT root-inducing powder (production of Beijing Ai Bidi research and development centre), spray gun (water gun, number of patent application: 201020504380.X), nozzle diameter 2.0mm.
Two, test method
1, inoculation liquid preparation:
Compound concentration is 2,6-dimethoxy 1,4-benzoquinone, the humic acid film of mass concentration 30g/l, the ABT rooting powder solution of mass concentration 50mg/L of 0.02mg/l, obtains inoculation liquid for subsequent use after equal-volume mixing.
2, seed paper is made by oneself:
The specification that paper (as newspaper, manuscript paper) is tailored into 30cm × 10cm will be commonly used, make paper-carrier, the lapping paper of same size is made with the soft paper (as toilet paper) of good permeability, inoculation liquid is mixed with viscosity earth, by weight the mixing of 1:30-40 ratio, obtain adhesiveness mud liquid, with hairbrush be stained with adhesiveness mud liquid paper-carrier brush is wet after, evenly will be sprinkling upon on paper-carrier through sprouting process Desert Herba Cistanches seed, density of sowing seeds is average 100/0.03m 2rapidly lapping paper concora crush is compressed at carrier wetted surface and paper-carrier, place dry place, dry, stack neatly for subsequent use, in April, 2012 inoculates 30 caves in Shaya County artificial planting Cakcayr Forest seed paper, earthing, fertilising, after inoculation after water head hydration moisture in the soil, irrigation management routinely, in April, 2014 investigates success ratio of inoculation;
3, scatter seeds process:
Sprout the cistanche deserticola seed after process through seed-soaking liquid, soak 5 days by inoculation liquid, dry in the shade for subsequent use; In April, 2012 inoculates 30 caves at Shaya County artificial planting Cakcayr Forest, earthing, fertilising, after inoculation after water head hydration moisture in the soil, and irrigation management routinely, in April, 2014 investigates success ratio of inoculation;
4, liquid inoculation method
1) in April, 2012 is at Shaya County artificial planting Cakcayr Forest apart from plant 60cm place, excavates the inoculation ditch of dark 70-80cm, ditches with mini-excavator.
2) in inoculation ditch, execute a small amount of fertilizer of people, will notice during fertilising that seed is isolated with fertile, with unburned kind, in ditch, execute farmyard manure 500g/m.
3) be sprayed in plantation ditch near host plant root system section side by inoculation liquid with through the seed of seed-soaking liquid sprouting process with spray gun, spray is connected on from the section of bottom of trench upwards 30-40cm, every meter controls ejection inoculation liquid 200-300ml, ejection seed 100-200 grain, often organize inoculation 10 cave, in triplicate, 30 caves are inoculated.
4) manually backfill is appropriate native immediately for the inoculation ditch inoculated, and backfill soil is treaded, and backfill soil is to hole along 10 cm places.
5), after soil has backfilled, after water head hydration moisture in the soil, irrigation management routinely, in April, 2014 investigates success ratio of inoculation.
Three, results and analysis:
The success ratio of inoculation comparing result of different vaccination method to Desert Herba Cistanches is as shown in table 3.
Table 3
embodiment 3:
One, test material:
1, cistanche deserticola seed process:
Within 2012, to gather in Habahe County halophilous herbage seed (Cistnche salsa (C.A.Mey.) G.Beck.), seed is carried out grain size grading screening, chooses the halophilous herbage high quality seed of diameter 0.55-0.7mm respectively; Cistanche deserticola seed sprouts process through seed-soaking liquid, dries in the shade for subsequent use.
2, medicament and instrument:
2, (abundant Rake bio tech ltd, Xinjiang buys 6-dimethoxy 1,4-benzoquinone (DMBQ), article number: D149705), humic acid film (Changzhou Yongfeng biologos element factory produce) and ABT root-inducing powder (production of Beijing Ai Bidi research and development centre), spray gun (water gun, number of patent application: 201020504380.X), nozzle diameter 2.0mm.
Two, test method
1, inoculation liquid preparation:
Compound concentration is 2,6-dimethoxy 1,4-benzoquinone, the humic acid film of mass concentration 30g/l, the ABT rooting powder solution of mass concentration 50mg/L of 0.02mg/l, obtains inoculation liquid for subsequent use after equal-volume mixing.
2, seed paper is made by oneself:
The specification that paper (as newspaper, manuscript paper) is tailored into 30cm × 10cm will be commonly used, make paper-carrier, the lapping paper of same size is made with the soft paper (as toilet paper) of good permeability, inoculation liquid is mixed with viscosity earth, by weight the mixing of 1:30-40 ratio, obtain adhesiveness mud liquid, with hairbrush be stained with adhesiveness mud liquid paper-carrier brush is wet after, evenly be sprinkling upon on paper-carrier by the halophilous herbage seed sprouting process through seed-soaking liquid, density of sowing seeds is average 100/0.03m 2rapidly lapping paper concora crush is compressed at carrier wetted surface and paper-carrier, place dry place, dry, stack neatly for subsequent use, in June, 2012, wild ceratoides latens seed paper inoculated 30 caves in Habahe County, earthing, fertilising, after inoculation after water head hydration moisture in the soil, irrigation management routinely, in May, 2014 investigates success ratio of inoculation;
3, scatter seeds process:
Sprout the cistanche deserticola seed after process through seed-soaking liquid, soak 5 days by inoculation liquid, dry in the shade for subsequent use; In June, 2012, wild ceratoides latens inoculated 30 caves in Habahe County, earthing, fertilising, after inoculation after water head hydration moisture in the soil, and irrigation management routinely, in May, 2014 investigates success ratio of inoculation;
4, liquid inoculation method
1) in June, 2012, wild ceratoides latens, apart from plant 60cm place, excavated the inoculation cave of dark 70-80cm, with manually carrying out out cave in Habahe County.
2) in inoculation cave, execute a small amount of fertilizer of people, will notice during fertilising that seed is isolated with fertile, with unburned kind, in cave, execute farmyard manure 100g/ cave.
3) be sprayed in kind of plant hole near host plant root system section side by inoculation liquid with through the seed of seed-soaking liquid sprouting process with spray gun, spray is connected on the section of upwards 30-40cm at the bottom of cave, every cave controls ejection inoculation liquid 40-60ml, ejection seed 20-50 grain, often organize inoculation 10 cave, in triplicate, 30 caves are inoculated.
4) manually backfill is appropriate native immediately in the inoculation cave inoculated, and backfill soil is treaded, and backfill soil is to hole along 10 cm places.
5), after soil has backfilled, after water head hydration moisture in the soil, irrigation management routinely, in May, 2014 investigates success ratio of inoculation.
Three, results and analysis:
The success ratio of inoculation comparing result of different vaccination method to halophilous herbage is as shown in table 4.
Table 4
embodiment 4:
One, test material:
1, cistanche deserticola seed process:
Within 2009, gathering Cistanche tubulosa seed (Cistanche tubulosa (schrenk) R.Wight) with field, seed being carried out grain size grading screening, chooses the Cistanche tubulosa high quality seed that diameter is greater than 0.7 mm respectively; Cistanche deserticola seed, through sprouting process, dries in the shade for subsequent use.
2, medicament and instrument:
2, (abundant Rake bio tech ltd, Xinjiang buys 6-dimethoxy 1,4-benzoquinone (DMBQ), article number: D149705), humic acid film (Changzhou Yongfeng biologos element factory produce) and ABT root-inducing powder (production of Beijing Ai Bidi research and development centre), spray gun (water gun, number of patent application: 201020504380.X), nozzle diameter is at 2.0mm.
Two, test method
1, inoculation liquid preparation:
Compound concentration is 2,6-dimethoxy 1,4-benzoquinone, the humic acid film of mass concentration 30g/l, the ABT rooting powder solution of mass concentration 50mg/L of 0.02mg/l, obtains inoculation liquid for subsequent use after equal-volume mixing.
2, seed paper is made by oneself:
The specification that paper (as newspaper, manuscript paper) is tailored into 30cm × 10cm will be commonly used, make paper-carrier, the lapping paper of same size is made with the soft paper (as toilet paper) of good permeability, inoculation liquid is mixed with viscosity earth, by weight the mixing of 1:30-40 ratio, obtain adhesiveness mud liquid, with hairbrush be stained with adhesiveness mud liquid paper-carrier brush is wet after, evenly be sprinkling upon on paper-carrier by the Cistanche tubulosa seed sprouting process through seed-soaking liquid, density of sowing seeds is average 100/0.03m 2rapidly lapping paper concora crush is compressed at carrier wetted surface and paper-carrier, place dry place, dry, stack neatly for subsequent use, in May, 2012 inoculates 30 caves in Huocheng County's artificial planting Chinese tamarisk woods seed paper, earthing, fertilising, after inoculation after water head hydration moisture in the soil, irrigation management routinely, in May, 2014 investigates success ratio of inoculation;
3, scatter seeds process:
Sprout the cistanche deserticola seed after process through seed-soaking liquid, soak 5 days by inoculation liquid, dry in the shade for subsequent use; In May, 2012 inoculates 30 caves, earthing, fertilising Huocheng County artificial planting Chinese tamarisk woods, after inoculation after water head hydration moisture in the soil, and irrigation management routinely, in May, 2014 investigates success ratio of inoculation;
4, liquid inoculation method
1) in May, 2012 is Huocheng County artificial planting Chinese tamarisk apart from plant 60cm place, excavates the inoculation ditch of dark 70-80cm, ditches with ditching machine.
2) in inoculation cave, execute a small amount of fertilizer of people, will notice during fertilising that seed is isolated with fertile, with unburned kind, in cave, execute farmyard manure 500g/m.
3) be sprayed in plantation ditch near host plant root system section side by inoculation liquid with through the seed of seed-soaking liquid sprouting process with spray gun, spray is connected on from the section of bottom of trench upwards 30-40cm, every meter controls ejection inoculation liquid 200-300ml, ejection seed 100-200 grain, often organize inoculation 10 cave, in triplicate, 30 caves are inoculated.
4) manually backfill is appropriate native immediately for the inoculation ditch inoculated, and backfill soil is treaded, and backfill soil is to hole along 10 cm places.
5), after soil has backfilled, after water head hydration moisture in the soil, irrigation management routinely, in May, 2014 investigates success ratio of inoculation.
Three, results and analysis:
The success ratio of inoculation comparing result of different vaccination method to Cistanche tubulosa is as shown in table 5.
Table 5
As can be seen from table 3,4,5, through preliminary investigation: trench digging directly broadcast sowing method, feature be easy to operate, efficiency is high and transfer period shorten, but need grain weight large, rate of vaccination is low; Seed paper inoculation method, feature is that transfer period is short, instant effect, but success ratio of inoculation is low, and inoculating surfaces is little, is the main inoculation method of artificial planting saline cistanche at present; Liquid inoculation method feature is planted for economizing, easy to operate, transfer period is short, rate of vaccination is the highest.
With the lance driving device of nozzle diameter at 2.0mm in liquid inoculation technology, spray kind of amount is moderate, and jeting effect is better, and spray and reduce sowing quantity, rate of vaccination is high, aborning handled easily, is easy to promote.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (9)

1. artificial planting saline cistanche liquid inoculation method, is characterized in that, comprise the following steps:
1) cistanche deserticola seed sizing screening and seed germination process;
2) transformation of classification inoculation apparatus;
3) preparation of inoculation liquid;
4) excavation inoculation ditch;
5) farmyard manure is executed;
6) liquid inoculation is carried out with inoculating facility;
7) backfill inoculation ditch;
8) after water head hydration moisture in the soil, irrigation management routinely.
2. artificial planting saline cistanche liquid inoculation method according to claim 1, it is characterized in that, in described step 1), cistanche deserticola seed sizing screening and seed germination process, concrete steps are as follows: cistanche deserticola seed is divided into three kinds, be respectively Desert Herba Cistanches, Cistanche tubulosa and halophilous herbage, high-quality cistanche deserticola seed after the screening of described particle diameter is respectively diameter and is more than or equal to the halophilous herbage high quality seed that Desert Herba Cistanches high quality seed O.7mm or Cistanche tubulosa high quality seed and diameter are 0.55-O.7 mm, cistanche deserticola seed is carried out grain size grading screening, the high-quality cistanche deserticola seed obtained is sprouted after process through seed-soaking liquid, for subsequent use after drying in the shade.
3. artificial planting saline cistanche liquid inoculation method according to claim 1, it is characterized in that, described step 2) in, the transformation of classification inoculation apparatus, concrete steps are as follows: classification inoculation apparatus selects the spray gun 1 of nozzle diameter 1.5-2.0mm, spray gun inner catheter diameter is more than or equal to 10mm, and extracts soft suction hose.
4. artificial planting saline cistanche liquid inoculation method according to claim 1, it is characterized in that, the preparation of inoculation liquid in described step 3), concrete steps are as follows: compound concentration is 2 of 0.02mg/l, the humic acid film of 6-dimethoxy 1,4-benzoquinone, mass concentration 30g/l, the ABT rooting powder solution of mass concentration 50mg/L, obtain inoculation liquid after equal-volume mixing for subsequent use.
5. artificial planting saline cistanche liquid inoculation method according to claim 1, it is characterized in that, excavation inoculation ditch in described step 4), concrete steps are as follows: at the host plant 60cm place that distance is selected, excavate the inoculation ditch of dark 70-80cm, ditch with mini-excavator or ditching machine.
6. artificial planting saline cistanche liquid inoculation method according to claim 1, it is characterized in that, execute farmyard manure in described step 5), concrete steps are as follows: in inoculation ditch, apply the fertilizer that becomes thoroughly decomposed on a small quantity, and seed is isolated with fertile, executes farmyard manure 500g/m in ditch.
7. artificial planting saline cistanche liquid inoculation method according to claim 1, it is characterized in that, carry out liquid inoculation with inoculating facility in described step 6), concrete steps are as follows:
A) selecting step 1) put into step 3) inoculation nutrient solution through the high-quality cistanche deserticola seed sprouting process, 1g seed puts into 15kg inoculation liquid;
B) step a) seed inoculation liquid is loaded step 2) liquid inoculation equipment, prepare inoculation;
C) liquid and seed to be sprayed near host plant root system section side in plantation ditch with step b) liquid inoculation equipment, spray is connected on from the section of bottom of trench upwards 30-40cm, and every meter controls ejection inoculation liquid 200-300ml, ejection seed 100-200 grain.
8. artificial planting saline cistanche liquid inoculation method according to claim 1, it is characterized in that, backfill inoculation ditch in described step 7), concrete steps are as follows: the inoculation ditch inoculated is immediately with machinery or the appropriate soil of artificial backfill, backfill soil is treaded, and backfill soil is to hole along 10 cm places.
9. artificial planting saline cistanche liquid inoculation method according to claim 1, it is characterized in that, in described step 8) after water head hydration moisture in the soil, irrigation management routinely, concrete steps are as follows: after soil has backfilled, after water head hydration moisture in the soil, irrigation management routinely, keeps soil moisture content at 20%-40%.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109418050A (en) * 2017-08-24 2019-03-05 中国科学院寒区旱区环境与工程研究所 Method for artificially cultivating cistanche
CN111517883A (en) * 2020-03-30 2020-08-11 新疆永林水草生态研究有限公司 Artificial cistanche salsa planting inoculant and application thereof

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