CN104447022A - Selenium-rich chitosan nutritional microbial fertilizer and preparation method thereof - Google Patents
Selenium-rich chitosan nutritional microbial fertilizer and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C05G3/80—Soil conditioners
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Abstract
The invention relates to a selenium-rich chitosan nutritional microbial fertilizer. The selenium-rich chitosan nutritional microbial fertilizer is prepared from the following raw materials: sodium selenite, chitosan hydrolysate, compound trace elements, monopotassium phosphate, urea, peptone, yeast powder, NaCl, NaOH, NaNO3, NaCl, NH4Cl, K2HPO4, a beef extract, glucose, starch, calcium carbonate, amino acid, potassium nitrate, ferrous sulfate, magnesium sulfate, trisodium citrate, biochemical enzymes and a mixed bacterium liquid. Due to addition of the chitosan, the viable count of a microbial agent can be kept; the efficacy of the chitosan for keeping the viable count is put into an effective play; due to addition of the chitosan, the amount of bacteria of the microbial agent can reach 20,000,000,000/mL; due to addition of the chitosan to a bacterial manure, the yield of commercial crops can also be increased by 10%-15%; and the nutritional microbial fertilizer has the efficacies of keeping moisture and slowly releasing fertilizer efficiency.
Description
Technical field
The present invention relates to a kind of selenium-rich nutritive type microbial fertilizer, specifically a kind of rich selenium chitosan nutritional type microbial fertilizer and preparation method, belong to technical field of biological fertilizer.
Background technology
China is a Ge Que selenium big country.GeneraI investigation proves, seriously in the Soils In The Region of China more than 70% lacks selenium, and in the grain of China more than 95% and livestock and poultry meat, selenium content is lower than 50 μ g/kg, belongs to Pin Xi big country.Every day, selenium intake only had 39 μ about g per capita in China, and not enough grownup triumph needs 80% of minimum 50 μ g/ days, larger apart from healthy requirement 100 ~ 200 μ g/ days differences; Farther apart from the 800 μ g that reach the standard grade safely.In China, except minority area, the population of 90% needs to adopt biotechnology supplement selenium.Not only people wants supplement selenium, and livestock and poultry also want supplement selenium.
China is large agricultural country, and chemical fertilizer plays an important role in China's agriculture production.But, along with the fertilizer amount of unit surface is more and more higher, there is the sight that serious soil " is more fed thinner ".What accompany with it is not only the degeneration of soil, the increase of applications of pesticide amount and the decline of quality of agricultural product, also has the underground water of nearly 40% because nitrate content is too high can not directly drink.Therefore, development environment friendly fertilizer is the development strategy of China.Selenium-enriched microbe bacterial manure because of its there is soil function improvement, increasing crop yield, quality of agricultural product improve, fertility is lasting and advantages of environment protection, and are subject to extensive concern.The microbial fertilizer history of existing more than 100 year so far, oneself in Asia, America, Europe And Africa etc. in the world more than 60 country apply.American and Britain, method, Deng developed country, increase the Innovation Input of microbial-bacterial fertilizer, obtain kind abundanter in recent years, the functionalization microbial-bacterial fertilizer that performance is more excellent, instead of the fertilizer application amount of about 40%.China also pays much attention to the development & application of green fertilizer, and microbial fertilizer industry achieves certain development, manufacturing enterprise more than 800, annual production nearly ten million ton, annual value of production crosses 10,000,000,000 yuan, but with the same period chemical fertilizer output compare with consumption, only account for less than 5%.But along with the raising that people require quality of the life, the appealing of developing green pollution-free food is more and more higher, this social demand will cause the market demand of microbial fertilizer increasing.
Selenium is one of necessary nutritional trace element of human body.Human or animal lacks or the selenium of excess intake, then can cause some disease, the sickness rate such as Keshan disease, Kaschin-Beck disease, myocardosis, cancer as scarce selenium area is very high.Research and practice proves, selenium element plays inherent keying action to HUMAN HEALTH, and it is glutathione peroxidase, thioredoxin reductase and subtract key component---the activator of the endogenous antioxidant such as iodine enzyme.It for adjustment human immune system, resist disease, delay senility, to prevent and the various diseases such as auxiliary for treating cancer plays an important role, be particularly that other antioxidants are incomparable and alternative in removing lipid free radical.
Selenium be just be found in recent decades very important, indispensable to HUMAN HEALTH, have the overall situation effect endogenous strong anti-oxidation material and free-radical scavengers.World Health Organization WHO announces: selenium is that life hits necessary trace element, selenium is respectfully called " kindling material of life " by domestic and international the world of medicine, enjoys the good reputation such as " long-lived element ", " anticancer king ", " heart patron saint ", " natural toxinicide ".Selenium has been known as by scientific and technological circle and " has contributed to the torment that the mankind exempt various slight illness, as unhealthy emotion, pancreatic disease, diabetes, reproductive system disease, virus infection and cancer etc." there is report, the people that in blood, selenium content is minimum dies from 3 times that cardiopathic probability is content height person.Selenium can make the immunity system of human body again rejuvenate.Selenium can discharge heavy metal of body toxin, and selenium is that seleno-protein runs into rapid combinations of poisonous metal ash such as lead, mercury, cadmium, stone roller and becomes harmless compounds and excrete with the form of selenium propylhomoserin composition seleno-protein in vivo.Selenium is the conditioning agent of iodine in body, and it plays very important regulating effect to iodine in thyroid hormone metabolism process.What be combined with selenium also has a kind of very important antioxidase---thioredoxin reductase, has a lot of biological function, affects the synthesis of nucleic acid.Selenium prophylaxis is infertile, and particularly prevent the symptoms such as the male sex dead essence, oligospermia, many data show, today infertility and scarce selenium have direct relation.
Man and animal takes in ensureing by way of the food mainly by containing selenium of selenium, and therefore, the preparation of rich selenium chitosan microbial preparation and the plantation of rich selenium product are very important.
Can the selenium that absorbs by human body be divided into elemental selenium, inorganic selenium and organoselenium three kinds.The feature of selenium is that between biological activity and toxicity, scope is extremely narrow, and for this reason, various countries scientist have studied many selenium compounds, attempts to find the selenium form that activity is high, toxicity is low.Along with the maturation of nanotechnology, the nanotechnology that scientist is advanced, zeroth order selenium (one of elemental selenium) is made nano particle selenium--nanometer selenium.Nanometer selenium, compared with general zeroth order selenium, there occurs the sudden change of biological property.Concrete following characteristics: 1) there is oxidation-resistance; 2) due to highly active characteristic, thus the hazardness of human body is greatly reduced, the nutrient contg that low dosage selenium is equivalent to high dosage in the past can be supplemented, thus avoid the side effect brought to human body.So nanometer selenium makes the structure of matter of selenium become the zeroth order material of nanomorphic by the compound of original molecular state, not only make human body fully absorb selenium, reduce its toxicity, but also possess good biological activity.
Chinese patent literature CN 102348516A (application number: 201110205067.5) disclose the efficient concentration selenium-rich agent that a kind of plant leaf surface absorbs, wherein containing high density selenium solution, amino acid nutrient element, absorption enhancers and be at least urea, phosphoric acid salt, vitriol, glucose, caramel one or several combination aid nutrition material; Wherein said high density selenium solution is the aqueous solution of selenium-containing compound, in selenium element mass percentage concentration for 0.1%-30%M/V.Application implementation efficient concentration selenium-rich agent of the present invention, compares to existing common rich selenium solution production selenium-enriched crop products efficiency of foliage-spray under equal ambient and improves 50%; Cost reduces 30%; And output volume increase 3-8%; Crop products selenium content can be made to bring up to 10-400 μ g/kg.But in this rich selenium preparation, selenium exists with inorganic selenium salt form, and inorganic selenium salt biological activity is low, does not have oxidation-resistance, and human body is very little to inorganic selenium salt specific absorption, and excessive amount has certain toxicity to human body, adds the hazardness to human body.
Chitosan is a kind of important derivatives of chitin, is the chitin product that deacetylation is transformed under highly basic effect.Difference between chitosan from chitin is mainly that de-acetyl degree is different, generally de-acetyl degree be greater than 70% call chitosan, chitosan derives from the exoskeleton of crustacean or insect, content is very abundant on earth has biocompatibility antimicrobial environment close friend due to chitosan and extracts the advantages such as convenient, be widely applied in a lot of fields, comprise wastewater treatment chromatography anti-biotic material Biodegradable films medicament carrier etc.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of rich selenium chitosan nutritional type microbial fertilizer, the present invention also provides a kind of preparation method of rich selenium chitosan nutritional type microbial fertilizer, containing elemental selenium in the present invention's rich selenium chitosan nutritional type microbial fertilizer, and elemental selenium is nano level, nontoxicity, human body is safe from harm, antagonistic action is had to harmful heavy metal, Degradation is had for the nitrate in vegetables, can be mixed with other fertilizer, good stability, add water and be applied to plant leaf surface, reach the rich selenium object of cereal, vegetables, fruit.And it contains a large amount of microorganism and causes farm crop to obtain the agricultural prods of the effects such as specific fertilizer effect, growth regulating effect, diseases prevention worm effect and soil organisms reparation with its vital movement and meta-bolites thereof.This selenium-rich nutritive type microbial fertilizer, it can improve soil, increases the quantity of soil microorganisms, improves disease-resistant, the anti-adversity ability of fruit-vegetable plant, improves the advantages such as utilization rate of fertilizer.
Technical scheme of the present invention is as follows:
A kind of rich selenium chitosan nutritional type microbial fertilizer, raw material is become to be grouped into by following by weight:
Sodium Selenite 8-15 part, chitosan hydrolyzate liquid 55-75 part, composite trace element 5-15 part, potassium primary phosphate 8-20 part, urea 10-25 part, peptone 8-20 part, yeast powder 4-10 part, NaCl 8-20 part, NaOH 8-15 part, NaNO
34-10 part, NaCl 10-25 part, NH
4cl 4-10 part, K
2hPO
48-20 part, extractum carnis 4-10 part, glucose 10-25 part, starch 10-25 part, calcium carbonate 8-20 part, amino acid/11 0-25 part, saltpetre 2-6 part, ferrous sulfate 2-6 part, magnesium sulfate 10-25 part, trisodium citrate 25-40 part, biochemical enzyme 10-25 part, mixed bacteria liquid 800-848 part;
Described chitosan hydrolyzate liquid be chitosan add water heating after, add phosphoric acid, abundant stirring reaction obtain;
Described mixed bacteria liquid is the mixed solution of bacillus amyloliquefaciens bacterium liquid, subtilis bacterium liquid, Bacillus licheniformis liquid, bacillus thuringiensis bacterium liquid and colloid bacillus cereus bacterium liquid.
The present invention is preferred, a kind of rich selenium chitosan nutritional type microbial fertilizer, and raw material is become to be grouped into by following by weight:
Sodium Selenite 10-12 part, chitosan hydrolyzate liquid 60-70 part, composite trace element 8-12 part, potassium primary phosphate 10-18 part, urea 15-20 part, peptone 12-18 part, yeast powder 6-8 part, NaCl 10-15 part, NaOH 10-12 part, NaNO
36-8 part, NaCl 15-20 part, NH
4cl 4-10 part, K
2hPO
412-18 part, extractum carnis 6-8 part, glucose 15-20 part, starch 15-20 part, calcium carbonate 12-18 part, amino acid/11 6-20 part, saltpetre 3-5 part, ferrous sulfate 3-5 part, magnesium sulfate 13-22 part, trisodium citrate 30-35 part, biochemical enzyme 18-25 part, mixed bacteria liquid 820-830 part.
The present invention is preferred, and described mixed bacteria liquid prepares by the following method:
(1) be that the inoculum size access of 0.25%-0.5% is equipped with in the fermentor tank of fermented liquid with volume ratio by bacillus amyloliquefaciens bacterial classification, aerobic fermentation 24-48h at 30-38 DEG C, obtained bacillus amyloliquefaciens bacterium liquid; Fermented liquid raw material forms: 8-10% soyflour, 4-7% molasses, 3-5% starch, and surplus is water, by weight percentage;
(2) by Bacillus subtilis strain with volume ratio be 0.25%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 24-48h at 30-38 DEG C, obtains subtilis bacterium liquid; Described fermented liquid raw material composition: soyflour 4-7%, glucose 4-8%, dipotassium hydrogen phosphate 1-2%, yeast extract paste 1-2%, magnesium sulfate 0.02-0.05%, calcium carbonate 0.3-0.6%, surplus is water, by weight percentage;
(3) by Bacillus licheniformis kind with volume ratio be 0.25%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 24-48h at 30-38 DEG C, obtains Bacillus licheniformis liquid; Described fermented liquid raw material composition: molasses 12-20%, W-Gum 8-12%, glucose 5-8%, surplus is water, by weight percentage;
(4) by bacillus thuringiensis bacterial classification with volume ratio be 0.25%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 24-48h at 30-38 DEG C, obtains bacillus thuringiensis bacterium liquid; Described fermented liquid raw material composition: molasses 12-18%, potassium hydroxide 3-5%, phosphoric acid 2-4%, urea 1-3%, extractum carnis 0.5-2%, magnesium sulfate 0.1-0.2%, zinc sulfate 0.1-0.2%, surplus is water, by weight percentage;
(5) by colloid bacillus cereus bacterial classification with volume ratio be 0.25%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 24-48h at 30-38 DEG C, obtains colloid bacillus cereus bacterium liquid; Described fermented liquid raw material composition: W-Gum 8-12%, extractum carnis 1-2%, glucose 1-3%, peptone 0.5-1%, calcium carbonate 0.5-1%, dipotassium hydrogen phosphate 0.5-1%, magnesium sulfate 0.02-0.05%, surplus is water, by weight percentage;
(6) by volume by bacillus amyloliquefaciens bacterium liquid: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: colloid bacillus cereus bacterium liquid is in (1-6): (1-3): (1-2): (1-2): the ratio mixing mixing of (1-2), obtains mixed bacteria liquid.
Preferred further, bacillus amyloliquefaciens bacterium liquid: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: the volume ratio of colloid bacillus cereus bacterium liquid is: (2-4): (2-3): 2:2:2.
Further preferred, the fermented liquid used in step (1), (2), (3), (4), (5) all sterilizings 2 ~ 3 hours at 110 DEG C ~ 120 DEG C, inoculate after cooling again.
The present invention is preferred, aerobic fermentation time 45-48h in step (1), (2), (3), (4), (5).
The present invention is preferred, in described bacillus amyloliquefaciens bacterium liquid, viable bacteria content is 120 ~ 15,000,000,000/ml, in subtilis bacterium liquid, viable bacteria content is 120 ~ 16,000,000,000/ml, in Bacillus licheniformis liquid, viable bacteria content is 120 ~ 15,000,000,000/ml, in bacillus thuringiensis bacterium liquid, viable bacteria content is 120 ~ 15,000,000,000/ml, and in colloid bacillus cereus bacterium liquid, viable bacteria content is 120 ~ 15,000,000,000/ml.
The present invention is preferred, described chitosan hydrolyzate liquid prepares by the following method: get chitosan, add chitosan weight 15-20 water doubly, mix, then 95-100 DEG C is heated to, add chitosan weight 13-18 phosphoric acid doubly again, fully stir and make it mixing, obtain chitosan hydrolyzate liquid.
The present invention is preferred, described composite trace element, be by etc. the following substances of quality mix: manganous sulfate, ferrous sulfate, boric acid, copper sulfate, zinc sulfate, ammonium molybdate.
The present invention is preferred, and described biochemical enzyme is phosphoglucokinase PEK, hexokinase, pyruvate kinase, pyruvate carboxylase, glutamate dehydrogenase, carbamyl phosphate synthase I or carbamyl phosphate synthase II.
The present invention also provides a kind of preparation method of rich selenium chitosan nutritional type microbial fertilizer, comprises step as follows:
By raw material Sodium Selenite, chitosan hydrolyzate liquid, composite trace element, potassium primary phosphate, urea, peptone, yeast powder, NaCl, NaOH, NaNO
3, NaCl, NH
4cl, K
2hPO
4, extractum carnis, glucose, starch, calcium carbonate, amino acid, saltpetre, ferrous sulfate, magnesium sulfate, trisodium citrate, biochemical enzyme, after mixed bacteria liquid mixes, regulate pH to 7 ~ 7.5, obtain mixture, by mixture in 30 ~ 35 DEG C under anaerobic through fermentation 24-48h, when the color of mixture changes to redness from faint yellow, i.e. obtained rich selenium chitosan nutritional type microbial fertilizer, described rich selenium chitosan nutritional type microbial fertilizer preparation is containing elemental selenium 0.3 ~ 0.4g/L, described selenium is nano level, median size is 60 ~ 150nm, active bacteria Zong Shuo≤15,000,000,000/m L.
The present invention is preferred, and mixture passes into rare gas element during the fermentation and maintains oxygen-free environment, and described rare gas element is nitrogen or argon gas.
The rich selenium chitosan nutritional type microbial fertilizer that the present invention obtains is liquid state, and solution takes on a red color and shows the existence of selenium nano particles.
The application of above-mentioned rich selenium chitosan nutritional type microbial fertilizer, proportion of crop planting fertilising for scarce selenium, poor selenium area is used, comprise root fertilising and leaf dressing, during root fertilising, rich selenium chitosan nutritional type microbial fertilizer is directly used, during leaf dressing, rich selenium chitosan nutritional type microbial fertilizer dilution 2 ~ 5 times is used.
The present invention is preferred, and described farm crop are grape, apple, summer squash, capsicum, cucumber, Chinese cabbage rape, tomato, eggplant.
Preferred further, described root with applying fertilizer rich selenium preparation Direct Uniform cloth is sprinkled upon in crop root part surrounding soil, earthing covers tightly again, or decile some ditches or hole, cave centered by crop root part, the even cloth of rich selenium preparation after dilution is sprinkled upon in bar ditch or hole, cave, earthing covers tightly again, rate of fertilizer application: every rich selenium preparation executed after 5 ~ 10 jin of dilutions.
The bacillus amyloliquefaciens that the present invention uses, subtilis, Bacillus licheniformis, bacillus thuringiensis, colloid bacillus cereus is existing conventional bacterial classification in prior art, can conventional market purchase obtain, the present invention's bacterial classification used is all purchased from Chinese Academy of Agricultural Sciences's agricultural Microbiological Culture Collection administrative center (public sale), wherein, bacillus amyloliquefaciens be numbered ACCC10167, the numbering ACCC04177 of subtilis, Bacillus licheniformis be numbered ACCC02936, bacillus thuringiensis be numbered ACCC11075, colloid bacillus cereus be numbered ACCC10013.
Component in fermented liquid involved in the present invention is existing conventional constituents in prior art, all conventionally prepares, such as, and soyflour, W-Gum, molasses, yeast extract paste, extractum carnis etc.
Sodium Selenite used in the present invention is purchased from Jinan Zhuan Meng Bioisystech Co., Ltd.
The present invention's chitosan used is purchased from Weifang Ke Hai chitin company limited, and animal blood lytic enzyme is purchased from Pangbo Bioengineering Co Ltd, Nanning.
In the chitosan solution of concentration of the present invention, the growth of chitosan to genus bacillus plays a driving role, because chitosan is polycation in the solution, when reaching certain high density, positive charge is given the cell surface of bacterium, more nutritive substance and chitosan itself is made to enter cell interior and the chitosan of certain high density may excite the activity of genus bacillus chitinase, make chitosan in cell by chitinase or chitoanase degrade, thus be utilized as nutritive substance, facilitate the growth of thalline.
In the present invention's rich selenium chitosan nutritional type microbial fertilizer, being added with of chitosan benefits control mankind cholesterol.Chitosan can stop the absorption of fat on the one hand, the Cholesterol Excretion in blood of human body can be fallen on the other hand.First, chitosan suppresses those to help the activity of the lipase of fat absorbing.Lipase reduces fat and human body is absorbed.Another one is excretion cholic acid.Once cholic acid excretion, then the cholesterol in blood is used to manufacture cholic acid.These two kinds of mechanism make chitosan become strong cholesterol scavenging agent.Chitosan is a kind of natural materials, has powerful Anion-adsorption power, is applicable to reduce cholesterol and without any side effect.
In the present invention's rich selenium chitosan nutritional type microbial fertilizer, being added with of chitosan benefits prevention and corntrol hypertension.Be exactly chlorion to one of the most influential factor of hypertension, it is taken in by salt usually.Angiotensin converting enzyme produces angiotensin II, and one causes vasoconstrictive material, and its vigor is from chlorion.Polymer chitosan plays a role as food fibre, is not absorbed in intestines.Chitosan passes through the adsorption between the chlorion of self and ammonia radical ion, excretion chlorion.Therefore, chitosan reduces angiotensin II.It contributes to preventing hypertension, particularly the crowd of those Excess free enthalpy salt.
In the present invention's rich selenium chitosan nutritional type microbial fertilizer, the interpolation of chitosan can keep the viable count in rich selenium chitosan nutritional type microbial fertilizer, the chitosan aqueous solution concentration used in this microbial inoculum reaches 10-20%, the interpolation of high density chitosan hydrolyzate liquid effectively can improve number of viable and extend the microbial survival cycle, it can extend microbial inoculum activity-maintaining time, thus can extend the preservation period to 2 year of microbial inoculum.The interpolation of chitosan can make the bacteria containing amount of microbiobacterial agent reach 20,000,000,000/mL.In bacterial manure, the interpolation of chitosan can make cash crop increase production 10-15%.
In the present invention's rich selenium chitosan nutritional type microbial fertilizer, chitosan is that natural plant nutrition urgees long agent--the raw material of foliage fertilizer, the foliage fertilizer composite by chitosan, can plant insecticide be given, disease-resistant, play the effect of fertilizer, plant and animal residues and micro-metals in soil can be decomposed again, thus be converted into the nutrient substance of plant, strengthen via plant immunity, promote the health of plant; Containing rich in protein, trace element in shrimp shell, crab shell, after animal eats absorption, there is good nutritive value.
In the present invention's rich selenium chitosan nutritional type microbial fertilizer, chitosan also has a significant effect to absorption in soil and excretion heavy metal.A remarkable characteristic of chitosan is adsorptive power.Many low-molecular-weight materials, such as metal ion, cholesterol, triglyceride level, cholic acid and organic mercury etc., can be adsorbed by chitosan.Particularly chitosan not only can adsorb magnesium, potassium, and can adsorb zinc, calcium, mercury and uranium.The adsorption activity of chitosan can play a role selectively.These metal ions concentration in human body is too high is harmful.Such as, in blood, cupric ion (Cu2+) excessive concentration can cause copper poisoning, even produces carcinogenic consequence.Now prove that chitosan is efficient inner complex medium.The size of the adsorptive power of chitosan depends on its deacetylation.Deacetylation is larger, and adsorptive power is stronger.
In the present invention's rich selenium chitosan nutritional type microbial fertilizer, chitosan can extend the freshness date of fruit and vegetable food.The object of preserving fruit and vegetable utilizing mainly keeps fruits and vegetables until shelf-lives after harvesting, can maintain normal quality, taste, nutritive ingredient and outward appearance, improve its commodity value.Containing chitosan in vegetables and fruits, fruits and vegetables tissue and structure aging can be slowed down, thus effectively prolongation fruits and vegetables adopt the rear life-span.Chitosan significantly can slow down the annesl of fruits and vegetables, also can be conducive to the hardness keeping fruit simultaneously.Chitosan concentration is higher, and fresh-keeping effect is better.Chitosan can hinder the ascorbic decline of storage bay, reduces the respiratory intensity of vegetables and fruits and reduces the Lipid peroxidation metabolism etc. adopting rear apple.After for some time preserved by vegetables and fruits containing chitosan, superoxide dismutase and Vitamin C content still keep higher level.
In the present invention's rich selenium chitosan nutritional type microbial fertilizer, also containing living microorganism, and farm crop can be caused to obtain the agricultural prods of the effects such as specific fertilizer effect, growth regulating effect, diseases prevention worm effect and soil organisms reparation with its vital movement and meta-bolites thereof, improving Soil structure and soil microenvironment, culture fertility, raising chemical fertilizer utilization ratio and reduction fertilizer and pesticide amount of application, preventing and treating in soil continuous cropping obstacle and raising crop yield quality etc. and can play the irreplaceable effect of other fertilizer.The root of plant can fix plant, moisture and the mineral salt be dissolved in the water and nitrogen can be absorbed again from soil, used for plant-growth, root system also can the material such as synthesizing amino acid, vegeto-alkali, organonitrogen, plant hormone, and healthy and strong flourishing root is that plant is necessary.
Beneficial effect
Preparation method's advantage of rich selenium chitosan nutritional type microbial fertilizer of the present invention is as follows:
1, the preparation method of rich selenium chitosan nutritional type microbial fertilizer of the present invention, fermentation time is short, only needs 24-48h, substantially increases the service efficiency of rich selenium chitosan microbial preparation, has saved cost.
2, the present invention adds the viable count that chitosan can keep microbiobacterial agent, effective effect playing chitosan and keep viable count.The chitosan aqueous solution concentration used in this microbial inoculum reaches 10-20%, the interpolation of high density chitosan hydrolyzate liquid effectively can improve number of viable and extend the microbial survival cycle, it can extend microbial inoculum activity-maintaining time, thus can extend the preservation period to 2 year of microbial inoculum.The interpolation of chitosan can make the bacteria containing amount of microbiobacterial agent reach 20,000,000,000/mL.In bacterial manure, the interpolation of chitosan can also make cash crop increase production 10-15%.The adding proportion of chitosan of the present invention is regioselective by long-term experiment, just can ensure that rich selenium preparation effectively improves the living environment of microorganism in soil, and has effect of moisture content maintenance and efficiency slow release.
3, chitosan is that natural plant nutrition urgees long agent--the raw material of foliage fertilizer, the foliage fertilizer composite by chitosan, plant insecticide can be given, disease-resistant, play the effect of fertilizer, plant and animal residues and micro-metals in soil can be decomposed again, thus be converted into the nutrient substance of plant, strengthen via plant immunity, promote the health of plant; Containing rich in protein, trace element in shrimp shell, crab shell, after animal eats absorption, there is good nutritive value.
4, chitosan also has a significant effect to absorption in soil and excretion heavy metal.A remarkable characteristic of chitosan is adsorptive power.Many low-molecular-weight materials, such as metal ion, cholesterol, triglyceride level, cholic acid and organic mercury etc., can be adsorbed by chitosan.Particularly chitosan not only can adsorb magnesium, potassium, and can adsorb zinc, calcium, mercury and uranium.
5, the interpolation of chitosan, can extend the freshness date of fruit and vegetable food.Fruits and vegetables can be kept until shelf-lives after harvesting, can normal quality, taste, nutritive ingredient and outward appearance be maintained, improve its commodity value.Containing chitosan in vegetables and fruits, fruits and vegetables tissue and structure aging can be slowed down, thus effectively prolongation fruits and vegetables adopt the rear life-span.Chitosan significantly can slow down the annesl of fruits and vegetables, also can be conducive to the hardness keeping fruit simultaneously.Chitosan concentration is higher, and fresh-keeping effect is better.Chitosan can hinder the ascorbic decline of storage bay.After for some time preserved by vegetables and fruits containing chitosan, superoxide dismutase and Vitamin C content still keep higher level.
6, the subtilis of this rich selenium chitosan microbial preparation use, bacillus amyloliquefaciens, bacillusmusilaginosiengineering, Bacillus licheniformis, bacillus thuringiensis colloid bacillus cereus bacterial classification, not only can carry the fermenting speed of selenium-enriched chitosan microbial preparation, and make the particle diameter of selenium nano particle more even, more be of value to the absorption of plant to this rich selenium chitosan microbial preparation.
7, the subtilis in the present invention's rich selenium chitosan nutritional type microbial fertilizer, bacillus amyloliquefaciens, bacillusmusilaginosiengineering, Bacillus licheniformis, bacillus thuringiensis colloid bacillus cereus bacterial classification, can bring beneficial effect to the microorganism in soil, it can increase the quantity of Soil Microorganism and improve the activity of Soil Microorganism.This type of bacterial classification causes farm crop to obtain specific fertilizer effect with its vital movement and meta-bolites thereof, growth regulating effect, the agricultural prods of the effects such as diseases prevention worm effect and soil organisms reparation
8, the present invention's rich selenium chitosan nutritional type microbial fertilizer, except containing selenium element, also containing the nitrogen that plant is required, phosphorus, potassium and trace element, have production-increasing function to vegetables and fruits.
6, rich selenium preparation of the present invention has degeneration-resistant effect for diseases and pests of agronomic crop, subtilis contained by it, bacillus amyloliquefaciens, bacillusmusilaginosiengineering, Bacillus licheniformis, bacillus thuringiensis colloid bacillus cereus has antagonistic action to harmful heavy metal, has Degradation for the nitrate in vegetables, can reduce applications of pesticide amount.
7, use rich selenium preparation for fertilizer, containing elemental selenium in rich selenium preparation, and elemental selenium is nano level, and the rich selenium product of planting out contains nano level elemental selenium, selenium content is at 30 ~ 50 micro-gs/kg, and mouthfeel is good, rich in sugar, and antioxidant property is good, nontoxicity, is safe from harm to human body
In a word, this rich selenium chitosan microbial preparation can improve soil, increase the quantity of soil microorganisms, improve resistance against diseases and the anti-adversity ability of farm crop, the existence of microorganism improves the utilization ratio of fertilizer, decrease the usage quantity of fertilizer, facilitate the rich selenium of plant, be beneficial to the absorption of nutrient, selenium-rich nutritive type microbial fertilizer of the present invention is liquid dosage form, can fill with root punching to execute and artificial foliage-spray and drip irrigation and Aircraft equalization, fertilization mode is simple.
Accompanying drawing explanation
Fig. 1 is the stereoscan photograph of the present invention's rich selenium chitosan nutritional type microbial fertilizer,
Fig. 2 is xps spectrogram (a) of the present invention's rich selenium chitosan nutritional type microbial fertilizer,
Fig. 3 is xps spectrogram (b) of the present invention's rich selenium chitosan nutritional type microbial fertilizer.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is described further, but institute of the present invention protection domain is not limited thereto.
Embodiment 1
A kind of rich selenium chitosan nutritional type microbial fertilizer, parts by weight of raw materials is as follows:
Sodium Selenite 8 parts, chitosan hydrolyzate liquid 55 parts, composite trace element 5 parts, potassium primary phosphate 8 parts, 10 parts, urea, peptone 8 parts, yeast powder 4 parts, NaCl 8 parts, NaOH 8 parts, NaNO
34 parts, NaCl 10 parts, NH
4cl 4 parts, K
2hPO
48 parts, extractum carnis 4 parts, glucose 10 parts, starch 10 parts, 8 parts, calcium carbonate, amino acid/11 0 part, 2 parts, saltpetre, 2 parts, ferrous sulfate, 10 parts, magnesium sulfate, trisodium citrate 25 parts, biochemical enzyme 10 parts, mixed bacteria liquid 800 parts;
Described mixed bacteria liquid prepares by the following method:
(1) bacillus amyloliquefaciens bacterial classification is equipped with in the fermentor tank of fermented liquid with the inoculum size access that volume ratio is 0.25%, aerobic fermentation 45h at 35 DEG C, obtained bacillus amyloliquefaciens bacterium liquid; Fermented liquid raw material forms: 8% soyflour, 4% molasses, 3% starch, and surplus is water, by weight percentage;
(2) be equipped with in the fermentor tank of fermented liquid by Bacillus subtilis strain with the inoculum size access that volume ratio is 0.25%, aerobic fermentation 45h at 35 DEG C, obtains subtilis bacterium liquid; Described fermented liquid raw material composition: soyflour 4%, glucose 4%, dipotassium hydrogen phosphate 1%, yeast extract paste 1%, magnesium sulfate 0.02%, calcium carbonate 0.3%, surplus is water, by weight percentage;
(3) be equipped with in the fermentor tank of fermented liquid by Bacillus licheniformis kind with the inoculum size access that volume ratio is 0.25%, aerobic fermentation 24h at 35 DEG C, obtains Bacillus licheniformis liquid; Described fermented liquid raw material composition: molasses 12%, W-Gum 8%, glucose 5%, surplus is water, by weight percentage;
(4) be equipped with in the fermentor tank of fermented liquid by bacillus thuringiensis bacterial classification with the inoculum size access that volume ratio is 0.25%, aerobic fermentation 45h at 35 DEG C, obtains bacillus thuringiensis bacterium liquid; Described fermented liquid raw material composition: molasses 12%, potassium hydroxide 3%, phosphoric acid 2%, urea 1%, extractum carnis 0.5%, magnesium sulfate 0.1%, zinc sulfate 0.1%, surplus is water, by weight percentage;
(5) be equipped with in the fermentor tank of fermented liquid by colloid bacillus cereus bacterial classification with the inoculum size access that volume ratio is 0.25%, aerobic fermentation 45h at 35 DEG C, obtains colloid bacillus cereus bacterium liquid; Described fermented liquid raw material composition: W-Gum 8%, extractum carnis 1%, glucose 1%, peptone 0.5%, calcium carbonate 0.5%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.02%, surplus is water, by weight percentage;
(6) step (1), (2), (3), (4), fermented liquid of using in (5) all sterilizings 2 hours at 120 DEG C, inoculate again after cooling, by volume by bacillus amyloliquefaciens bacterium liquid: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: colloid bacillus cereus bacterium liquid, in the ratio mixing mixing of 4:3:2:2:2, obtains mixed bacteria liquid.
Described chitosan hydrolyzate liquid prepares by the following method: get chitosan, adds the water of chitosan weight 15 times, mixes, then 98 DEG C are heated to, add the phosphoric acid of chitosan weight 18 times again, fully stir and make it mixing, obtain chitosan hydrolyzate liquid.
Described composite trace element, be by etc. the following substances of quality mix: manganous sulfate, ferrous sulfate, boric acid, copper sulfate, zinc sulfate, ammonium molybdate.
Described biochemical enzyme is phosphoglucokinase PEK.
Preparation method:
By raw material Sodium Selenite, chitosan hydrolyzate liquid, composite trace element, potassium primary phosphate, urea, peptone, yeast powder, NaCl, NaOH, NaNO
3, NaCl, NH
4cl, K
2hPO
4, extractum carnis, glucose, starch, calcium carbonate, amino acid, saltpetre, ferrous sulfate, magnesium sulfate, trisodium citrate, biochemical enzyme, after mixed bacteria liquid mixes, regulate pH to 7.5, obtain mixture, through fermentation 30h under mixture is passed into nitrogen maintenance oxygen free condition in 32 DEG C, when the color of mixture changes to redness from faint yellow, i.e. obtained rich selenium chitosan nutritional type microbial fertilizer, described rich selenium chitosan nutritional type microbial fertilizer preparation is containing elemental selenium 0.3 ~ 0.4g/L, described selenium is nano level, median size is 100nm, active bacteria Zong Shuo≤15,000,000,000/m L.
Fig. 1 is the stereoscan photograph of the present invention's rich selenium chitosan nutritional type microbial fertilizer, and as can be seen from the figure, the median size of nano level elemental selenium is at 100nm.
Fig. 2 (a) shows, under XPS spectrum, and C1s, O1s in solution, the existence of N1s and Se3d.But Se3d is not obvious in XPS spectrum, namely the intensity of its emission peak is very weak, and this may be because selenium nano particle is easily caught by the protein excretion of genus bacillus.
Fig. 3 (b) shows, and under XPS spectrum, the Se3d in solution combines can at 55.74eV, and it is the characteristic peak of selenium element itself.The existence of C, N, O may be the existence because protein in sample and other biological macromole pollute.
We can draw the following conclusions from XPS spectrum, this Reactive Synthesis selenium element, and by undersized nano granules of selenium that the protein excretion of genus bacillus covers.In in the forming process of M-Se, protein plays an important role.
Embodiment 2
Rich selenium chitosan nutritional type microbial fertilizer as described in Example 1, difference is, parts by weight of raw materials is as follows:
Sodium Selenite 10 parts, chitosan hydrolyzate liquid 60 parts, composite trace element 8 parts, potassium primary phosphate 10 parts, 15 parts, urea, peptone 12 parts, yeast powder 6 parts, NaCl 10 parts, NaOH 10 parts, NaNO
36 parts, NaCl 15 parts, NH
4cl 4 parts, K
2hPO
412 parts, extractum carnis 6 parts, glucose 15 parts, starch 15 parts, 12 parts, calcium carbonate, amino acid/11 6 parts, 3 parts, saltpetre, 3 parts, ferrous sulfate, 13 parts, magnesium sulfate, trisodium citrate 30 parts, biochemical enzyme 18 parts, mixed bacteria liquid 820 parts.
Bacillus amyloliquefaciens bacterium liquid in mixed bacteria liquid: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: the volume ratio of colloid bacillus cereus bacterium liquid is: 6:3:2:2:2.
Chitosan hydrolyzate liquid prepares by the following method: get chitosan, adds the water of chitosan weight 18 times, mixes, then 100 DEG C are heated to, add the phosphoric acid of chitosan weight 16 times again, fully stir and make it mixing, obtain chitosan hydrolyzate liquid.
Preparation method is with embodiment 1.
Embodiment 3
Rich selenium chitosan nutritional type microbial fertilizer as described in Example 1, difference is, parts by weight of raw materials is as follows:
Sodium Selenite 12 parts, chitosan hydrolyzate liquid 70 parts, composite trace element 12 parts, potassium primary phosphate 18 parts, 20 parts, urea, peptone 18 parts, yeast powder 8 parts, NaCl 15 parts, NaOH 12 parts, NaNO
38 parts, NaCl 20 parts, NH
4cl 10 parts, K
2hPO
418 parts, extractum carnis 8 parts, glucose 20 parts, starch 20 parts, 18 parts, calcium carbonate, 20 parts, amino acid, 5 parts, saltpetre, 5 parts, ferrous sulfate, 22 parts, magnesium sulfate, trisodium citrate 35 parts, biochemical enzyme 25 parts, mixed bacteria liquid 830 parts.
Bacillus amyloliquefaciens bacterium liquid in mixed bacteria liquid: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: the volume ratio of colloid bacillus cereus bacterium liquid is: 1:1:1:1:1.
Embodiment 4
Rich selenium chitosan nutritional type microbial fertilizer as described in Example 1, difference is, parts by weight of raw materials is as follows:
Sodium Selenite 15 parts, chitosan hydrolyzate liquid 75 parts, composite trace element 15 parts, potassium primary phosphate 20 parts, 25 parts, urea, peptone 20 parts, yeast powder 10 parts, NaCl20 part, NaOH 15 parts, NaNO
310 parts, NaCl 25 parts, NH
4cl10 part, K
2hPO
420 parts, extractum carnis 10 parts, glucose 25 parts, starch 25 parts, 20 parts, calcium carbonate, 25 parts, amino acid, 6 parts, saltpetre, 6 parts, ferrous sulfate, 25 parts, magnesium sulfate, trisodium citrate 40 parts, biochemical enzyme 25 parts, mixed bacteria liquid 848 parts.
Claims (10)
1. a rich selenium chitosan nutritional type microbial fertilizer, raw material is become to be grouped into by following by weight:
Sodium Selenite 8-15 part, chitosan hydrolyzate liquid 55-75 part, composite trace element 5-15 part, potassium primary phosphate 8-20 part, urea 10-25 part, peptone 8-20 part, yeast powder 4-10 part, NaCl 8-20 part, NaOH 8-15 part, NaNO
34-10 part, NaCl 10-25 part, NH
4cl 4-10 part, K
2hPO
48-20 part, extractum carnis 4-10 part, glucose 10-25 part, starch 10-25 part, calcium carbonate 8-20 part, amino acid/11 0-25 part, saltpetre 2-6 part, ferrous sulfate 2-6 part, magnesium sulfate 10-25 part, trisodium citrate 25-40 part, biochemical enzyme 10-25 part, mixed bacteria liquid 800-848 part;
Described chitosan hydrolyzate liquid be chitosan add water heating after, add phosphoric acid, abundant stirring reaction obtain;
Described mixed bacteria liquid is the mixed solution of bacillus amyloliquefaciens bacterium liquid, subtilis bacterium liquid, Bacillus licheniformis liquid, bacillus thuringiensis bacterium liquid and colloid bacillus cereus bacterium liquid.
2. rich selenium chitosan nutritional type microbial fertilizer according to claim 1, is characterized in that, raw material is become to be grouped into by following by weight:
Sodium Selenite 10-12 part, chitosan hydrolyzate liquid 60-70 part, composite trace element 8-12 part, potassium primary phosphate 10-18 part, urea 15-20 part, peptone 12-18 part, yeast powder 6-8 part, NaCl 10-15 part, NaOH 10-12 part, NaNO
36-8 part, NaCl 15-20 part, NH
4cl 4-10 part, K
2hPO
412-18 part, extractum carnis 6-8 part, glucose 15-20 part, starch 15-20 part, calcium carbonate 12-18 part, amino acid/11 6-20 part, saltpetre 3-5 part, ferrous sulfate 3-5 part, magnesium sulfate 13-22 part, trisodium citrate 30-35 part, biochemical enzyme 18-25 part, mixed bacteria liquid 820-830 part.
3. rich selenium chitosan nutritional type microbial fertilizer according to claim 1, it is characterized in that, described mixed bacteria liquid prepares by the following method:
(1) be that the inoculum size access of 0.25%-0.5% is equipped with in the fermentor tank of fermented liquid with volume ratio by bacillus amyloliquefaciens bacterial classification, aerobic fermentation 24-48h at 30-38 DEG C, obtained bacillus amyloliquefaciens bacterium liquid; Fermented liquid raw material forms: 8-10% soyflour, 4-7% molasses, 3-5% starch, and surplus is water, by weight percentage;
(2) by Bacillus subtilis strain with volume ratio be 0.25%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 24-48h at 30-38 DEG C, obtains subtilis bacterium liquid; Described fermented liquid raw material composition: soyflour 4-7%, glucose 4-8%, dipotassium hydrogen phosphate 1-2%, yeast extract paste 1-2%, magnesium sulfate 0.02-0.05%, calcium carbonate 0.3-0.6%, surplus is water, by weight percentage;
(3) by Bacillus licheniformis kind with volume ratio be 0.25%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 24-48h at 30-38 DEG C, obtains Bacillus licheniformis liquid; Described fermented liquid raw material composition: molasses 12-20%, W-Gum 8-12%, glucose 5-8%, surplus is water, by weight percentage;
(4) by bacillus thuringiensis bacterial classification with volume ratio be 0.25%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 24-48h at 30-38 DEG C, obtains bacillus thuringiensis bacterium liquid; Described fermented liquid raw material composition: molasses 12-18%, potassium hydroxide 3-5%, phosphoric acid 2-4%, urea 1-3%, extractum carnis 0.5-2%, magnesium sulfate 0.1-0.2%, zinc sulfate 0.1-0.2%, surplus is water, by weight percentage;
(5) by colloid bacillus cereus bacterial classification with volume ratio be 0.25%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 24-48h at 30-38 DEG C, obtains colloid bacillus cereus bacterium liquid; Described fermented liquid raw material composition: W-Gum 8-12%, extractum carnis 1-2%, glucose 1-3%, peptone 0.5-1%, calcium carbonate 0.5-1%, dipotassium hydrogen phosphate 0.5-1%, magnesium sulfate 0.02-0.05%, surplus is water, by weight percentage;
(6) by volume by bacillus amyloliquefaciens bacterium liquid: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: colloid bacillus cereus bacterium liquid is in (1-6): (1-3): (1-2): (1-2): the ratio mixing mixing of (1-2), obtains mixed bacteria liquid.
4. rich selenium chitosan nutritional type microbial fertilizer according to claim 3, it is characterized in that, bacillus amyloliquefaciens bacterium liquid: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: the volume ratio of colloid bacillus cereus bacterium liquid is: (2-4): (2-3): 2:2:2.
5. rich selenium chitosan nutritional type microbial fertilizer according to claim 1, it is characterized in that, the all sterilizings 2 ~ 3 hours at 110 DEG C ~ 120 DEG C of step (1), (2), (3), (4), fermented liquid of using in (5), inoculate again after cooling, aerobic fermentation time 45-48h in step (1), (2), (3), (4), (5).
6. rich selenium chitosan nutritional type microbial fertilizer according to claim 1, it is characterized in that, in described bacillus amyloliquefaciens bacterium liquid, viable bacteria content is 120 ~ 15,000,000,000/ml, in subtilis bacterium liquid, viable bacteria content is 120 ~ 16,000,000,000/ml, in Bacillus licheniformis liquid, viable bacteria content is 120 ~ 15,000,000,000/ml, in bacillus thuringiensis bacterium liquid, viable bacteria content is 120 ~ 15,000,000,000/ml, and in colloid bacillus cereus bacterium liquid, viable bacteria content is 120 ~ 15,000,000,000/ml.
7. rich selenium chitosan nutritional type microbial fertilizer according to claim 1, it is characterized in that, described chitosan hydrolyzate liquid prepares by the following method: get chitosan, add chitosan weight 15-20 water doubly, mix, be then heated to 95-100 DEG C, then add chitosan weight 13-18 phosphoric acid doubly, abundant stirring makes it mixing, obtains chitosan hydrolyzate liquid.
8. rich selenium chitosan nutritional type microbial fertilizer according to claim 1, is characterized in that, described composite trace element, be by etc. the following substances of quality mix: manganous sulfate, ferrous sulfate, boric acid, copper sulfate, zinc sulfate, ammonium molybdate.
9. rich selenium chitosan nutritional type microbial fertilizer according to claim 1, it is characterized in that, described biochemical enzyme is phosphoglucokinase PEK, hexokinase, pyruvate kinase, pyruvate carboxylase, glutamate dehydrogenase, carbamyl phosphate synthase I or carbamyl phosphate synthase II.
10. the preparation method of a kind of rich selenium chitosan nutritional type microbial fertilizer according to claim 1, comprises step as follows:
By raw material Sodium Selenite, chitosan hydrolyzate liquid, composite trace element, potassium primary phosphate, urea, peptone, yeast powder, NaCl, NaOH, NaNO
3, NaCl, NH
4cl, K
2hPO
4, extractum carnis, glucose, starch, calcium carbonate, amino acid, saltpetre, ferrous sulfate, magnesium sulfate, trisodium citrate, biochemical enzyme, after mixed bacteria liquid mixes, regulate pH to 7 ~ 7.5, obtain mixture, by mixture in 30 ~ 35 DEG C under anaerobic through fermentation 24-48h, when the color of mixture changes to redness from faint yellow, i.e. obtained rich selenium chitosan nutritional type microbial fertilizer, described rich selenium chitosan nutritional type microbial fertilizer preparation is containing elemental selenium 0.3 ~ 0.4g/L, described selenium is nano level, median size is 60 ~ 150nm, active bacteria Zong Shuo≤15,000,000,000/mL.
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| CN112481166B (en) * | 2020-12-04 | 2024-05-28 | 西安恒田生物科技有限公司 | Method for synthesizing biological nano-selenium by using bacillus methylotrophicus LW-6 and application thereof |
| CN113319110A (en) * | 2021-05-18 | 2021-08-31 | 昆明理工大学 | Method for restoring heavy metal contaminated soil and increasing fertilizer by spraying waste mash of alcohol |
| CN113956097A (en) * | 2021-11-30 | 2022-01-21 | 淮安大华生物科技有限公司 | Preparation method of disease-resistant biological nano selenium fertilizer for rice |
| CN114751389A (en) * | 2022-04-27 | 2022-07-15 | 广东省地质实验测试中心 | Selenium-based modified nano hydroxyapatite material, preparation method thereof and application thereof in Cd-contaminated soil remediation |
| CN114751389B (en) * | 2022-04-27 | 2023-10-03 | 广东省地质实验测试中心 | Selenium-based modified nano hydroxyapatite material, preparation method thereof and application thereof in restoring Cd-polluted soil |
| CN116177760A (en) * | 2023-04-27 | 2023-05-30 | 河北华邦生物科技有限公司 | Microecological preparation for aquaculture tail water treatment |
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