CN109867569A - A kind of selenium-rich type microbial-bacterial fertilizer and preparation method thereof - Google Patents
A kind of selenium-rich type microbial-bacterial fertilizer and preparation method thereof Download PDFInfo
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- CN109867569A CN109867569A CN201910325938.3A CN201910325938A CN109867569A CN 109867569 A CN109867569 A CN 109867569A CN 201910325938 A CN201910325938 A CN 201910325938A CN 109867569 A CN109867569 A CN 109867569A
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 76
- 239000011669 selenium Substances 0.000 title claims abstract description 69
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 64
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 64
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 66
- 229940091258 selenium supplement Drugs 0.000 claims abstract description 63
- 229910052742 iron Inorganic materials 0.000 claims abstract description 33
- 239000002105 nanoparticle Substances 0.000 claims abstract description 33
- 235000013557 nattō Nutrition 0.000 claims abstract description 28
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 26
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 25
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 25
- 244000005700 microbiome Species 0.000 claims abstract description 21
- 230000000855 fungicidal effect Effects 0.000 claims abstract description 19
- 239000000417 fungicide Substances 0.000 claims abstract description 19
- 239000011573 trace mineral Substances 0.000 claims abstract description 17
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 17
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims abstract description 16
- 239000000618 nitrogen fertilizer Substances 0.000 claims abstract description 16
- 239000002686 phosphate fertilizer Substances 0.000 claims abstract description 16
- 229940072033 potash Drugs 0.000 claims abstract description 16
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims abstract description 16
- 235000015320 potassium carbonate Nutrition 0.000 claims abstract description 16
- 229920000805 Polyaspartic acid Polymers 0.000 claims abstract description 13
- 238000010521 absorption reaction Methods 0.000 claims abstract description 13
- 230000000813 microbial effect Effects 0.000 claims abstract description 13
- 108010064470 polyaspartate Proteins 0.000 claims abstract description 13
- 150000004676 glycans Chemical class 0.000 claims abstract description 12
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 12
- 239000005017 polysaccharide Substances 0.000 claims abstract description 12
- 239000003610 charcoal Substances 0.000 claims abstract description 11
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims abstract description 11
- 238000002156 mixing Methods 0.000 claims abstract description 11
- 239000011781 sodium selenite Substances 0.000 claims abstract description 11
- 229960001471 sodium selenite Drugs 0.000 claims abstract description 11
- 235000015921 sodium selenite Nutrition 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 239000000203 mixture Substances 0.000 claims description 17
- 239000008367 deionised water Substances 0.000 claims description 16
- 229910021641 deionized water Inorganic materials 0.000 claims description 16
- 229940024606 amino acid Drugs 0.000 claims description 15
- 150000001413 amino acids Chemical class 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 claims description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 11
- 244000060011 Cocos nucifera Species 0.000 claims description 10
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 10
- 239000011790 ferrous sulphate Substances 0.000 claims description 10
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 10
- 239000002054 inoculum Substances 0.000 claims description 10
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 claims description 10
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 10
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 10
- 238000013019 agitation Methods 0.000 claims description 9
- 229910052799 carbon Inorganic materials 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 9
- 230000001580 bacterial effect Effects 0.000 claims description 8
- 239000002068 microbial inoculum Substances 0.000 claims description 8
- 239000005696 Diammonium phosphate Substances 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical group [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 6
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 6
- 235000019838 diammonium phosphate Nutrition 0.000 claims description 6
- 241001474374 Blennius Species 0.000 claims description 5
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 229910052796 boron Inorganic materials 0.000 claims description 5
- 239000011575 calcium Substances 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 239000004202 carbamide Substances 0.000 claims description 5
- 229940047608 chelated magnesium Drugs 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 210000003608 fece Anatomy 0.000 claims description 5
- 239000010871 livestock manure Substances 0.000 claims description 5
- 238000011068 loading method Methods 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 5
- 238000001694 spray drying Methods 0.000 claims description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea group Chemical group NC(=O)N XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 5
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 4
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 4
- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 4
- 239000006012 monoammonium phosphate Substances 0.000 claims description 4
- 239000001103 potassium chloride Substances 0.000 claims description 3
- 235000011164 potassium chloride Nutrition 0.000 claims description 3
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 3
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 3
- 235000011151 potassium sulphates Nutrition 0.000 claims description 3
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 claims 1
- 244000003416 Asparagus officinalis Species 0.000 claims 1
- 235000005340 Asparagus officinalis Nutrition 0.000 claims 1
- 229940038879 chelated zinc Drugs 0.000 claims 1
- 239000010903 husk Substances 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 abstract description 8
- 239000000126 substance Substances 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 12
- 241000196324 Embryophyta Species 0.000 description 11
- 241000209140 Triticum Species 0.000 description 10
- 235000021307 Triticum Nutrition 0.000 description 10
- 239000001888 Peptone Substances 0.000 description 8
- 108010080698 Peptones Proteins 0.000 description 8
- 235000015278 beef Nutrition 0.000 description 8
- 235000019319 peptone Nutrition 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 239000004615 ingredient Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 239000005909 Kieselgur Substances 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 238000002242 deionisation method Methods 0.000 description 4
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 4
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 229910052698 phosphorus Inorganic materials 0.000 description 4
- 239000011574 phosphorus Substances 0.000 description 4
- 229910052710 silicon Inorganic materials 0.000 description 4
- 239000010703 silicon Substances 0.000 description 4
- UOXSXMSTSYWNMH-UHFFFAOYSA-L zinc;2-aminoacetate Chemical group [Zn+2].NCC([O-])=O.NCC([O-])=O UOXSXMSTSYWNMH-UHFFFAOYSA-L 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 235000013312 flour Nutrition 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 206010039921 Selenium deficiency Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000002526 effect on cardiovascular system Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 208000019926 Keshan disease Diseases 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 108010074686 Selenoproteins Proteins 0.000 description 1
- 102000008114 Selenoproteins Human genes 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 239000012084 conversion product Substances 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 235000014106 fortified food Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000518 rheometry Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 229940082569 selenite Drugs 0.000 description 1
- MCAHWIHFGHIESP-UHFFFAOYSA-L selenite(2-) Chemical compound [O-][Se]([O-])=O MCAHWIHFGHIESP-UHFFFAOYSA-L 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Fertilizers (AREA)
Abstract
The present invention discloses a kind of selenium-rich type microbial-bacterial fertilizer and preparation method thereof, belongs to technical field of fertilizers.Fertilizer of the present invention is prepared by the raw material of following parts by weight: 140-160 parts of nitrogenous fertilizer, 40-60 parts of phosphate fertilizer, 80-100 parts of potash fertilizer, trace element chelated fertilizer 20-30 parts middle, 5-10 parts of charcoal, 1-3 parts of poly-aspartic-acid, 1-3 parts of algal polysaccharides, 1-3 parts of complex micro organism fungicide, 0.5-1.5 parts of sodium selenite, 3-5 parts of iron nano-particle;The complex micro organism fungicide is that candida utili, bacillus subtilis and bafillus natto are obtained by mixing according to mass ratio 1.5-2.5:1:2.5-3.5.The present invention mainly utilizes microbial activities to promote crop to the absorption efficiency of selenium, while the nutrients such as scientific matching NPK, provides basal nutrient substance abundant for crop.The formula of the invention is reasonable, and preparation process is simple, and fertilizer efficiency is significant, safety and environmental protection.
Description
Technical field
The invention belongs to technical field of fertilizers, and in particular to a kind of selenium-rich type microbial-bacterial fertilizer and preparation method thereof.
Background technique
Selenium is the essential trace elements of the human body, is the important component of part antioxidase and selenoprotein in human body.It grinds
Study carefully and shows that selenium has a variety of important physiological functions, such as healthy, the anti-oxidant, cancer-resisting that protection is cardiovascular and myocardium.People
Body selenium deficiency will lead to such as a variety of diseases of Keshan disease, cardiovascular and cerebrovascular diseases, cancer, hypertension.Selenium can not generate in vivo, human body institute
The selenium needed can only be absorbed from food.The area of China 72% belongs to selenium deficiency or low selenium area, and national about 2/3rds populations are deposited
It is insufficient in different degrees of selenium intake.Although selenium is not nutrient necessary to plant, plant is that nature selenium ecology is followed
The direct sources of key link and human body selenium in ring.Se content is generally relatively low in natural food, is given birth to by technique of enriched selenium
It is selenium-supply mode common at present that object, which is strengthened,.Currently, selenium-rich meat products, microorganism is that the functional food of carrier has all obtained accordingly
Research and development, and the most commonly used carrier of selenium-enriched food is mostly plant food.Plant has to be absorbed by root and blade
The characteristics of microelement can absorb using plant and be enriched with inorganic states selenium, and be translated into easy for plant to absorpt utilize
The characteristic of form selenium carries out research and the corresponding product of research and development of external source selenium-rich.Strengthen selenium to plant using selenium-rich fertilizer to have been demonstrated
It is a kind of safe, low cost, efficient and convenient selenium-rich means.In low selenium area, the adjusting of plant Se content can take root application, leaf
Face the methods of sprays, soaks seed or dress seed, and the Se content at the positions such as root, stem, leaf, the fruit of plant is improved, to meet humans and animals
Psychological need.
Although selenium is not nutrient necessary to plant, with to selenium in further investigation agriculturally, the kind of selenium-rich fertilizer
Class is also varied.Blade face selenium-rich fertilizer can be divided by the difference of fertilization mode and base applies selenium-rich fertilizer;It can be divided by material situation solid
Body selenium-rich fertilizer, liquid selenium-rich fertilizer;According to the chemical component of fertilizer, inorganic selenium-rich fertilizer, organic selenium-rich fertilizer can be divided into.In recent years,
Occurs the novel selenium-enriched fertilizer of a batch again.
Biological selenium-rich fertilizer refers to biological agent using biotechnology manufacture, having specific fertilizer efficiency to crop, effectively
Ingredient can be specific living organism, the metabolin of organism or conversion product of matrix etc., and this organism is either micro-
Biology is also possible to animal and plant tissue and cell.However, Biological Selenium fertilizer bioavailability is not high at present, fertilizer efficiency is not significant enough,
Higher cost, market application is insufficient, it is still desirable to further to improve.
Summary of the invention
To solve problems of the prior art, a kind of selenium-rich type microbial-bacterial fertilizer is provided, the fertilizer efficiency is significant, at
This is cheap, is suitble to all kinds of crops to use, and can promote crop quality and yield, improves soil environment, has wide city
Field application value.The present invention also provides the preparation methods of the microbial-bacterial fertilizer.
The technical scheme adopted by the invention is that:
A kind of selenium-rich type microbial-bacterial fertilizer is prepared by the raw material of following parts by weight: 140-160 parts of nitrogenous fertilizer, phosphate fertilizer
40-60 parts, 80-100 parts of potash fertilizer, trace element chelated fertilizer 20-30 parts middle, 5-10 parts of charcoal, 1-3 parts of poly-aspartic-acid,
1-3 parts of algal polysaccharides, 1-3 parts of complex micro organism fungicide, 0.5-1.5 parts of sodium selenite, 3-5 parts of iron nano-particle;It is described compound
Microbial bacterial agent is candida utili, bacillus subtilis and bafillus natto according to mass ratio 1.5-2.5:1:2.5-
3.5 being obtained by mixing.
The middle trace element chelated fertilizer is zinc-amino acid chelate, amino acid chelated magnesium, amino acid chelated calcium and amino acid
Chelating boron is obtained by mixing according to mass ratio 1:1.6:1.8:2.
The iron nano-particle is to be prepared by the following steps and obtain: ferrous sulfate 20g and iron chloride 20g being taken to be dissolved in 500mL
In deionized water, 20g coconut shell powder active carbon, magnetic agitation 60min is added, heated solution stirs lower be added dropwise to 70 DEG C
Then mixture is aged 5h in 100 DEG C of water-baths, is cooled to room temperature, uses deionization by the sodium hydroxide solution of 100mL5mol/L
Water washed product repeatedly, in baking oven at 80 DEG C dry 4h to get arriving iron nano-particle.
The nitrogenous fertilizer is urea, and the phosphate fertilizer is that perhaps the Diammonium phosphate (DAP) potash fertilizer is potassium chloride or sulphur to monoammonium phosphate
Sour potassium.
A kind of preparation method of selenium-rich type microbial-bacterial fertilizer, including with step:
(1) microbial bacterial agent is prepared:
Using triangular flask, culture medium loading amount is the one third of triangular flask, and sterilize 30min at 120 DEG C, is connect respectively after cooling
Kind candida utili, bacillus subtilis and bafillus natto, inoculum concentration 2-5%, and 180rpm is trained at 28-32 DEG C
Support 15h;Then according to 3% inoculum concentration, access sterilized seed tank culture base, 28 DEG C of cultivation temperature, cultivate under 180rpm
For 24 hours, candida utili, bacillus subtilis and bafillus natto bacterium solution are respectively obtained;
(2) candida utili obtained by step (1), bacillus subtilis and bafillus natto bacterium solution are used into silicon respectively
Diatomaceous earth absorption, plate and frame filter press filters pressing after the completion of absorption, spray drying, 130 DEG C of inlet air temperature, feed rate 10ml/min, point
Candida utili, bacillus subtilis and bafillus natto microbial inoculum are not obtained, then according to mass ratio 1.5-2.5:1:2.5-
3.5 mixing, obtain complex micro organism fungicide;
(3) it prepares iron nano-particle: ferrous sulfate 20g and iron chloride 20g being taken to be dissolved in 500mL deionized water, 20g is added
It is molten to stir the lower sodium hydroxide that 100mL5mol/L is added dropwise to 70 DEG C for coconut shell powder active carbon, magnetic agitation 60min, heated solution
Then mixture is aged 5h in 100 DEG C of water-baths, is cooled to room temperature by liquid, with deionized water washed product repeatedly, 80 in baking oven
4h is dried at DEG C to get iron nano-particle is arrived;
(4) by weight by nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, middle trace element chelated fertilizer, charcoal, poly-aspartic-acid, seaweed
Polysaccharide, complex micro organism fungicide, sodium selenite, iron nano-particle are uniformly mixed, and are added in airslide disintegrating mill, and fineness control exists
300-500 mesh, is granulated subsequently into pelletizer, is dried after the completion of being granulated, it is micro- that selenium-rich type of the present invention is obtained after drying
Bio-bacterial manure.
Wherein, in step (1), triangular flask culture medium is beef-protein medium, concrete composition are as follows: beef extract 5g/
L, peptone 10g/L, NaCl5g/L, agar 15g/L, remaining is water.
The ingredient of seed tank culture base are as follows: beef extract 0.3~0.8%, peptone 0.7~1.2%, glucose 0.1~
0.6%, NaCl 0.1~0.6%, MgSO4·7H 2O 0.01~0.06%, K2HPO40.01%~0.06%, MnSO4
0.001%~0.008%, Yu Weishui, pH6.8-7.2.
Candida utili, bacillus subtilis and bafillus natto kind used in the present invention are commercially available gained.
The utility model has the advantages that the nutrients such as scientific matching NPK of the present invention, provide basal nutrient substance abundant for crop.
The present invention is a kind of microbial bacterial agent, and main to promote crop to the absorption efficiency of selenium using microbial activities, the present invention utilizes production
Protein Candida, bacillus subtilis and bafillus natto can promote the inorganic selenium in soil to convert as mixed bacteria
For organic selenium, promotes crop to absorb, reduce the usage amount of inorganic selenium, while the active material of microorganism secretion, can be promoted
Crop yield promotes crop quality.The present invention also adds iron nano-particle, can be in nutrients release while providing ferro element
In the process, adsorption sustained-release is carried out to nutrient, avoiding selenite, to the pollution of soil, is peace under incomplete absorbing state
The increasing selenium mode that loopful is protected.The mutual cooperation of microbial bacterial agent and porous magnetic particle jointly promotes selenium element and efficiently utilizes.
The present invention adds poly-aspartic-acid and algal polysaccharides etc. and is used as fertilizer synergist, especially poly-aspartic acid band
The poly-aspartic-acid of negative electrical charge, macromolecular can have certain suction-operated to negative ions, can with algal polysaccharides interaction
Promote selenium element efficiently to be absorbed by crops, and itself is a kind of nontoxic degradable environmentally friendly product.
The formula of the invention is reasonable, and preparation process is simple, and fertilizer efficiency is significant, and safety and environmental protection, has a vast market using latent
Power.
Specific embodiment
Technical scheme is described further combined with specific embodiments below, but not limited to this.
Embodiment 1
A kind of selenium-rich type microbial-bacterial fertilizer is prepared by the raw material of following parts by weight: 140 parts of nitrogenous fertilizer, 40 parts of phosphate fertilizer,
80 parts of potash fertilizer, 20 parts of middle trace element chelated fertilizer, 5 parts of charcoal, 1 part of poly-aspartic-acid, 1 part of algal polysaccharides, composite microbial
1 part of object microbial inoculum, 0.5 part of sodium selenite, 3 parts of iron nano-particle;The complex micro organism fungicide is candida utili, withered grass bud
Spore bacillus and bafillus natto are obtained by mixing according to mass ratio 1.5:1:2.5.
The middle trace element chelated fertilizer is zinc-amino acid chelate, amino acid chelated magnesium, amino acid chelated calcium and amino acid
Chelating boron is obtained by mixing according to mass ratio 1:1.6:1.8:2.
The iron nano-particle is to be prepared by the following steps and obtain: ferrous sulfate 20g and iron chloride 20g being taken to be dissolved in 500mL
In deionized water, 20g coconut shell powder active carbon, magnetic agitation 60min is added, heated solution stirs lower be added dropwise to 70 DEG C
Then mixture is aged 5h in 100 DEG C of water-baths, is cooled to room temperature, uses deionization by the sodium hydroxide solution of 100mL5mol/L
Water washed product repeatedly, in baking oven at 80 DEG C dry 4h to get arriving iron nano-particle.
The nitrogenous fertilizer is urea, and the phosphate fertilizer is monoammonium phosphate, and the potash fertilizer is potassium chloride.
A kind of preparation method of selenium-rich type microbial-bacterial fertilizer, including with step:
(1) microbial bacterial agent is prepared:
Using triangular flask, culture medium loading amount is the one third of triangular flask, and sterilize 30min at 120 DEG C, is connect respectively after cooling
Kind candida utili, bacillus subtilis and bafillus natto, inoculum concentration 2%, and 180rpm is cultivated at 28-32 DEG C
15h;Then according to 3% inoculum concentration, access sterilized seed tank culture base, 28 DEG C of cultivation temperature, cultivate under 180rpm
For 24 hours, candida utili, bacillus subtilis and bafillus natto bacterium solution are respectively obtained;
(2) candida utili obtained by step (1), bacillus subtilis and bafillus natto bacterium solution are used into silicon respectively
Diatomaceous earth absorption, plate and frame filter press filters pressing after the completion of absorption, spray drying, 130 DEG C of inlet air temperature, feed rate 10ml/min, point
Candida utili, bacillus subtilis and bafillus natto microbial inoculum are not obtained, are then mixed according to mass ratio 1.5:1:2.5,
Obtain complex micro organism fungicide;
(3) it prepares iron nano-particle: ferrous sulfate 20g and iron chloride 20g being taken to be dissolved in 500mL deionized water, 20g is added
It is molten to stir the lower sodium hydroxide that 100mL5mol/L is added dropwise to 70 DEG C for coconut shell powder active carbon, magnetic agitation 60min, heated solution
Then mixture is aged 5h in 100 DEG C of water-baths, is cooled to room temperature by liquid, with deionized water washed product repeatedly, 80 in baking oven
4h is dried at DEG C to get iron nano-particle is arrived;
(4) by weight by nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, middle trace element chelated fertilizer, charcoal, poly-aspartic-acid, seaweed
Polysaccharide, complex micro organism fungicide, sodium selenite, iron nano-particle are uniformly mixed, and are added in airslide disintegrating mill, and fineness control exists
300-500 mesh, is granulated subsequently into pelletizer, is dried after the completion of being granulated, it is micro- that selenium-rich type of the present invention is obtained after drying
Bio-bacterial manure.
Wherein, in step (1), triangular flask culture medium is beef-protein medium, concrete composition are as follows: beef extract 5g/
L, peptone 10g/L, NaCl5g/L, agar 15g/L, remaining is water.
The ingredient of seed tank culture base are as follows: beef extract 0.3~0.8%, peptone 0.7~1.2%, glucose 0.1~
0.6%, NaCl0.1~0.6%, MgSO4·7H2O 0.01~0.06%, K2HPO40.01%~0.06%, MnSO4
0.001%~0.008%, Yu Weishui, pH6.8-7.2.
Embodiment 2
A kind of selenium-rich type microbial-bacterial fertilizer is prepared by the raw material of following parts by weight: 150 parts of nitrogenous fertilizer, 50 parts of phosphate fertilizer,
90 parts of potash fertilizer, 25 parts of middle trace element chelated fertilizer, 8 parts of charcoal, 2 parts of poly-aspartic-acid, 2 parts of algal polysaccharides, composite microbial
2 parts of object microbial inoculum, 1 part of sodium selenite, 4 parts of iron nano-particle;The complex micro organism fungicide is candida utili, withered grass gemma
Bacillus and bafillus natto are obtained by mixing according to mass ratio 2:1:3.
The middle trace element chelated fertilizer is zinc-amino acid chelate, amino acid chelated magnesium, amino acid chelated calcium and amino acid
Chelating boron is obtained by mixing according to mass ratio 1:1.6:1.8:2.
The iron nano-particle is to be prepared by the following steps and obtain: ferrous sulfate 20g and iron chloride 20g being taken to be dissolved in 500mL
In deionized water, 20g coconut shell powder active carbon, magnetic agitation 60min is added, heated solution stirs lower be added dropwise to 70 DEG C
Then mixture is aged 5h in 100 DEG C of water-baths, is cooled to room temperature, uses deionization by the sodium hydroxide solution of 100mL5mol/L
Water washed product repeatedly, in baking oven at 80 DEG C dry 4h to get arriving iron nano-particle.
The nitrogenous fertilizer is urea, and the phosphate fertilizer is Diammonium phosphate (DAP), and the potash fertilizer is potassium sulfate.
A kind of preparation method of selenium-rich type microbial-bacterial fertilizer, including with step:
(1) microbial bacterial agent is prepared:
Using triangular flask, culture medium loading amount is the one third of triangular flask, and sterilize 30min at 120 DEG C, is connect respectively after cooling
Kind candida utili, bacillus subtilis and bafillus natto, inoculum concentration 3.5%, and 180rpm is trained at 28-32 DEG C
Support 15h;Then according to 3% inoculum concentration, access sterilized seed tank culture base, 28 DEG C of cultivation temperature, cultivate under 180rpm
For 24 hours, candida utili, bacillus subtilis and bafillus natto bacterium solution are respectively obtained;
(2) candida utili obtained by step (1), bacillus subtilis and bafillus natto bacterium solution are used into silicon respectively
Diatomaceous earth absorption, plate and frame filter press filters pressing after the completion of absorption, spray drying, 130 DEG C of inlet air temperature, feed rate 10ml/min, point
Candida utili, bacillus subtilis and bafillus natto microbial inoculum are not obtained, are then mixed, are obtained according to mass ratio 2:1:3
Complex micro organism fungicide;
(3) it prepares iron nano-particle: ferrous sulfate 20g and iron chloride 20g being taken to be dissolved in 500mL deionized water, 20g is added
It is molten to stir the lower sodium hydroxide that 100mL5mol/L is added dropwise to 70 DEG C for coconut shell powder active carbon, magnetic agitation 60min, heated solution
Then mixture is aged 5h in 100 DEG C of water-baths, is cooled to room temperature by liquid, with deionized water washed product repeatedly, 80 in baking oven
4h is dried at DEG C to get iron nano-particle is arrived;
(4) by weight by nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, middle trace element chelated fertilizer, charcoal, poly-aspartic-acid, seaweed
Polysaccharide, complex micro organism fungicide, sodium selenite, iron nano-particle are uniformly mixed, and are added in airslide disintegrating mill, and fineness control exists
300-500 mesh, is granulated subsequently into pelletizer, is dried after the completion of being granulated, it is micro- that selenium-rich type of the present invention is obtained after drying
Bio-bacterial manure.
Wherein, in step (1), triangular flask culture medium is beef-protein medium, concrete composition are as follows: beef extract 5g/
L, peptone 10g/L, NaCl5g/L, agar 15g/L, remaining is water.
The ingredient of seed tank culture base are as follows: beef extract 0.3~0.8%, peptone 0.7~1.2%, glucose 0.1~
0.6%, NaCl0.1~0.6%, MgSO4·7H 2O 0.01~0.06%, K2HPO4 0.01%~0.06%, MnSO4
0.001%~0.008%, Yu Weishui, pH6.8-7.2.
Embodiment 3
A kind of selenium-rich type microbial-bacterial fertilizer is prepared by the raw material of following parts by weight: 160 parts of nitrogenous fertilizer, 60 parts of phosphate fertilizer,
It is 100 parts of potash fertilizer, 30 parts of middle trace element chelated fertilizer, 10 parts of charcoal, 3 parts of poly-aspartic-acid, 3 parts of algal polysaccharides, compound micro-
3 parts of bacteria agent, 1.5 parts of sodium selenite, 5 parts of iron nano-particle;The complex micro organism fungicide is candida utili, withered grass
Bacillus and bafillus natto are obtained by mixing according to mass ratio 2.5:1:3.5.
The middle trace element chelated fertilizer is zinc-amino acid chelate, amino acid chelated magnesium, amino acid chelated calcium and amino acid
Chelating boron is obtained by mixing according to mass ratio 1:1.6:1.8:2.
The iron nano-particle is to be prepared by the following steps and obtain: ferrous sulfate 20g and iron chloride 20g being taken to be dissolved in 500mL
In deionized water, 20g coconut shell powder active carbon, magnetic agitation 60min is added, heated solution stirs lower be added dropwise to 70 DEG C
Then mixture is aged 5h in 100 DEG C of water-baths, is cooled to room temperature, uses deionization by the sodium hydroxide solution of 100mL5mol/L
Water washed product repeatedly, in baking oven at 80 DEG C dry 4h to get arriving iron nano-particle.
The nitrogenous fertilizer is urea, and the phosphate fertilizer is monoammonium phosphate, and the potash fertilizer is potassium sulfate.
A kind of preparation method of selenium-rich type microbial-bacterial fertilizer, including with step:
(1) microbial bacterial agent is prepared:
Using triangular flask, culture medium loading amount is the one third of triangular flask, and sterilize 30min at 120 DEG C, is connect respectively after cooling
Kind candida utili, bacillus subtilis and bafillus natto, inoculum concentration 5%, and 180rpm is cultivated at 28-32 DEG C
15h;Then according to 3% inoculum concentration, access sterilized seed tank culture base, 28 DEG C of cultivation temperature, cultivate under 180rpm
For 24 hours, candida utili, bacillus subtilis and bafillus natto bacterium solution are respectively obtained;
(2) candida utili obtained by step (1), bacillus subtilis and bafillus natto bacterium solution are used into silicon respectively
Diatomaceous earth absorption, plate and frame filter press filters pressing after the completion of absorption, spray drying, 130 DEG C of inlet air temperature, feed rate 10ml/min, point
Candida utili, bacillus subtilis and bafillus natto microbial inoculum are not obtained, are then mixed according to mass ratio 2.5:1:3.5,
Obtain complex micro organism fungicide;
(3) it prepares iron nano-particle: ferrous sulfate 20g and iron chloride 20g being taken to be dissolved in 500mL deionized water, 20g is added
It is molten to stir the lower sodium hydroxide that 100mL5mol/L is added dropwise to 70 DEG C for coconut shell powder active carbon, magnetic agitation 60min, heated solution
Then mixture is aged 5h in 100 DEG C of water-baths, is cooled to room temperature by liquid, with deionized water washed product repeatedly, 80 in baking oven
4h is dried at DEG C to get iron nano-particle is arrived;
(4) by weight by nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, middle trace element chelated fertilizer, charcoal, poly-aspartic-acid, seaweed
Polysaccharide, complex micro organism fungicide, sodium selenite, iron nano-particle are uniformly mixed, and are added in airslide disintegrating mill, and fineness control exists
300-500 mesh, is granulated subsequently into pelletizer, is dried after the completion of being granulated, it is micro- that selenium-rich type of the present invention is obtained after drying
Bio-bacterial manure.
Wherein, in step (1), triangular flask culture medium is beef-protein medium, concrete composition are as follows: beef extract 5g/
L, peptone 10g/L, NaCl5g/L, agar 15g/L, remaining is water.
The ingredient of seed tank culture base are as follows: beef extract 0.3~0.8%, peptone 0.7~1.2%, glucose 0.1~
0.6%, NaCl0.1~0.6%, MgSO4·7H 2O 0.01~0.06%, K2HPO40.01%~0.06%, MnSO4
0.001%~0.008%, Yu Weishui, pH6.8-7.2.
Comparative example 1
This comparative formulation and preparation method, except the part of addition and preparation without complex micro organism fungicide, remaining is same
Embodiment 3.
Comparative example 2
This comparative formulation and preparation method, except the part of addition and preparation without iron nano-particle, remaining is the same as implementation
Example 3.
Product quality inspection
Product is carried out according to (GB20287-2006) " agricultural microbial agent " method, to microbial inoculum obtained by embodiment 1-3 into
Row measurement, measurement result such as table 1:
1 testing result of table
Embodiment 1 | Embodiment 2 | Embodiment 3 | |
Living bacteria count | 4.2 hundred million/gram | 4.3 hundred million/gram | 4.5 hundred million/gram |
Moisture | 5.2 | 6.3 | 6.0 |
Appearance | Particle is uniform | Particle is uniform | Particle is uniform |
Miscellaneous bacteria rate | 0.9% | 1.0% | 1.1% |
The experiment of crop field fertilizer efficiency
It tests and is carried out in 2018-2019 in Weifang City, Shandong Province planting base, be experimental field loam, 0-20cm topsoil
The content of organic matter 2.01%, full nitrogen 0.125%, alkali-hydrolyzable nitrogen 92.02mg/kg, full phosphorus (P2O5) 0.085%, rapid available phosphorus (P2O5)
20.63mg/kg, available potassium (K2O)120.2mg/kg.Experimental cultivar: wheat.
Experiment sets 6 processing altogether, repeats three times, totally 18 pieces of processing fields.
S1-S6, S1-S3 apply selenium-rich type microbial-bacterial fertilizer obtained by 1-3 of the embodiment of the present invention, amount of application 100kg/hm2;
S4-S5 applies selenium-rich type microbial-bacterial fertilizer obtained by comparative example 1-2 of the present invention, amount of application 200kg/hm2;
S6 is blank control group, does not apply any fertilizer.
Wheat aging time investigates wheat biology and economic characters, comprising: seed Se content (uses cyanide atomic fluorescence spectrophotometry
GB/T5009.93-2010), the silty tensile property of wheat flour is (special with the physical property water absorption rate and rheology of wheat flour dough
The measurement farinograph method G B/T 14614-2006 of property, the measurement tensilometer method of the physical characteristic rheological properties of wheat flour dough
) and the performance indicators such as volume increase, mass of 1000 kernel GB/T14615-2006.
Specific experiment is as a result, as shown in the table:
1 wheat quality experimental result of table
2 wheat performance test result of table
Compared with blank control group, wheat obtained by group of the embodiment of the present invention, fringe grain quality be substantially better than comparative example and
Blank control group, Se content are obviously improved, and volume increase reaches 15% or more.And also has positive shadow to the silty performance of wheat
It rings, the stress strain curve area (powder power) and ductility relative increase of dough, enhanced stretch resistance and the opposite reduction of stretch ratio.Usual feelings
Under condition, the dough that powder power is big and stretch ratio is moderate has good edible quality.
It should be noted that above-described embodiment is only the section Example for realizing preferred embodiment of the invention, Er Feiquan
Portion's embodiment.Obviously, it is based on the above embodiment of the present invention, those of ordinary skill in the art are not making creative work
Under the premise of other all embodiments obtained, should fall within the scope of the present invention.
Claims (5)
1. a kind of selenium-rich type microbial-bacterial fertilizer, which is characterized in that be to be prepared by the raw material of following parts by weight: nitrogenous fertilizer 140-
160 parts, 40-60 parts of phosphate fertilizer, 80-100 parts of potash fertilizer, trace element chelated fertilizer 20-30 parts middle, 5-10 parts of charcoal, poly- lucid asparagus
1-3 parts of propylhomoserin, 1-3 parts of algal polysaccharides, 1-3 parts of complex micro organism fungicide, 0.5-1.5 parts of sodium selenite, iron nano-particle 3-5
Part;The complex micro organism fungicide is candida utili, bacillus subtilis and bafillus natto according to mass ratio 1.5-
2.5:1:2.5-3.5 being obtained by mixing.
2. selenium-rich type microbial-bacterial fertilizer according to claim 1, which is characterized in that the middle trace element chelated fertilizer is amino
Sour chelated zinc, amino acid chelated magnesium, amino acid chelated calcium and chelating amino acids boron according to mass ratio 1:1.6:1.8:2 mix and
?.
3. selenium-rich type microbial-bacterial fertilizer according to claim 1, which is characterized in that the iron nano-particle is by following steps
It is prepared: ferrous sulfate 20g and iron chloride 20g being taken to be dissolved in 500mL deionized water, 20g coconut shell powder active carbon, magnetic is added
Power stirs 60min, and heated solution stirs the lower sodium hydroxide solution that 100mL5mol/L is added dropwise, then mixture is existed to 70 DEG C
Be aged 5h in 100 DEG C of water-baths, be cooled to room temperature, with deionized water washed product repeatedly, in baking oven at 80 DEG C dry 4h to get arriving
Iron nano-particle.
4. selenium-rich type microbial-bacterial fertilizer according to claim 1, which is characterized in that the nitrogenous fertilizer is urea, and the phosphate fertilizer is
Perhaps the Diammonium phosphate (DAP) potash fertilizer is potassium chloride or potassium sulfate to monoammonium phosphate.
5. the preparation method of selenium-rich type microbial-bacterial fertilizer described in a kind of claim 1-4 any one, which is characterized in that including with
Step:
(1) microbial bacterial agent is prepared:
Using triangular flask, culture medium loading amount is the one third of triangular flask, and sterilize 30min at 120 DEG C, is inoculated with and produces respectively after cooling
Protein Candida, bacillus subtilis and bafillus natto, inoculum concentration 2-5%, and 180rpm is cultivated at 28-32 DEG C
15h;Then according to 3% inoculum concentration, access sterilized seed tank culture base, 28 DEG C of cultivation temperature, cultivate under 180rpm
For 24 hours, candida utili, bacillus subtilis and bafillus natto bacterium solution are respectively obtained;
(2) candida utili obtained by step (1), bacillus subtilis and bafillus natto bacterium solution are used into diatomite respectively
Absorption, plate and frame filter press filters pressing after the completion of absorption, spray drying, 130 DEG C of inlet air temperature, feed rate 10ml/min, respectively
Candida utili, bacillus subtilis and bafillus natto microbial inoculum, it is then mixed according to mass ratio 1.5-2.5:1:2.5-3.5
It closes, obtains complex micro organism fungicide;
(3) it prepares iron nano-particle: ferrous sulfate 20g and iron chloride 20g being taken to be dissolved in 500mL deionized water, 20g coconut husk is added
Powdered Activated Carbon, magnetic agitation 60min, heated solution stir the lower sodium hydroxide solution that 100mL5mol/L is added dropwise to 70 DEG C,
Then mixture is aged 5h in 100 DEG C of water-baths, be cooled to room temperature, with deionized water washed product repeatedly, 80 DEG C in baking oven
Lower dry 4h to get arrive iron nano-particle;
(4) by weight nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, middle trace element chelated fertilizer, charcoal, poly-aspartic-acid, seaweed is more
Sugar, complex micro organism fungicide, sodium selenite, iron nano-particle are uniformly mixed, and are added in airslide disintegrating mill, and fineness control exists
300-500 mesh, is granulated subsequently into pelletizer, is dried after the completion of being granulated, it is micro- that selenium-rich type of the present invention is obtained after drying
Bio-bacterial manure.
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