CN104221717B - A kind of improve the method for cordycepin content in Cordyceps militaris (L.) Link.sporophore - Google Patents

Abstract

The invention discloses and a kind of improve the method for cordycepin content in Cordyceps militaris (L.) Link.sporophore, the present invention is by using zinc ion to coerce cordycepin, adjustment in combination with illumination condition, gluconic acid zinc solution is sprayed in the particular growth stage of Cordyceps sporophore, can bring and strong coerce effect, sporophore cordycepin content, up to 0.25 0.35%, the most compared to existing technology, can shorten the incubation time of more than a week.

Description

A kind of improve the method for cordycepin content in Cordyceps militaris (L.) Link.sporophore

Technical field

The present invention relates to Artificial Planting Method of C. militarles field, particularly relate to a kind of Artificial Control and cultivate bar Part is to improve the method for cordycepin content in Cordyceps militaris (L.) Link.sporophore.

Background technology

(Cordyceps mi1itaris, has another name called Cordyceps militaris (L.) Link. to Cordyceps militaris (L.) Link., belongs on fungal taxonomy Ascomycotina, gang pyrenomycetes, Ergota Zoopagales, Clavicipitaceae, Cordyceps, with Cordyceps (Cordyceps Sinensis) one of Cordyceps sinensis fungus it is all.Cordyceps sinensis fungus is to parasitize insecticide, Aranea or big The general designation of the class entomogenous fungi complex formed on the underground sporophore of group's capsule platymiscium.Cordyceps militaris (L.) Link. is Cordyceps Belonging to the type sepecies of fungus, main host plants is in Lepidoptera, coleoptera and more dipterous insecticides in the wild Larva and pupa on, it be that " sweet in the mouth, property are flat, have lung benefiting kidney, mend smart that the classic of TCM records its effect Marrow, hemostasis and phlegm ", basically identical with the function of Cordyceps.

Cordycepin is one of active ingredient main in Cordyceps militaris (L.) Link., and cordycepin (cordycepin) has another name called 3 ｀ mono-deoxyadenosines, be Cuningham in a kind of adenosine class active substance of nineteen fifty-one isolated, Molecular formula is C₁₀H₁₃N₅0₃, molecular weight is 251.24, fusing point 230-231 DEG C.Cordycepin is in Cordyceps militaris (L.) Link. One of main active ingredient, have anti-bacteria and anti-virus, antitumor and resisting fatigue, defying age, Antiinflammatory improves human body immunity, suppresses activity of monoamine oxidase, has higher medical value with wide General pharmacological action.Clinically main application for the treatment of cancer, leukemia, expansion bronchus, significantly increase The kidney tonifying effect of waiting for a long time plain, anti-ageing.

In the past, owing to Cordyceps militaris (L.) Link. yield in the wild is rare, its exploitation are not valued by the people. But from late nineteen nineties in last century, since its extensive artificial culture succeeds, it develops valency Being worth more and more recognized, the research especially for cordycepin gets more and more.In artificial culture Improve cordycepin content production application be broadly divided into raising liquid fermentation and culture thing in cordycepin content, Improve cordycepin content and three kinds of approach of cordycepin content in raising sporophore in solid fermentation culture, Wherein liquid fermentation and culture and solid fermentation are cultivated mainly to obtain cordycepin sterling as target.And improve In sporophore, cordycepin content is then with the commercial quality improving Cordyceps militaris (L.) Link.sporophore as target.Improving In the method for cordycepin content, current existing technology mainly can be summarized as the following aspects: 1, logical Cross induction mutation of bacterium and selection-breeding improves cordycepin content；2, by culture medium Middle nutrition optimizing components is carried High cordycepin content；3, by adding precursor raising cordycepin content in the medium；4, pass through Fungal elicitor improves cordycepin content.At present, the worm grass product developed gets more and more, and market needs Asking the most increasing, the artificial culture of Cordyceps militaris (L.) Link.sporophore is just becoming one and is having very big economic worth How new industry, improve cordycepin content in Cordyceps militaris (L.) Link.sporophore and have good market prospect.

Summary of the invention

It is an object of the invention to provide and a kind of improve the method for cordycepin content in Cordyceps militaris (L.) Link.sporophore, it Mainly by Artificial Control to improve cordycepin content, the pupa used in the method and prior art The cultural method of Cordyceps sporophore and the method for raising cordycepin content can use simultaneously.

The present invention is achieved by the following technical solution:

1), mycelia culture: finished bottle is put in 15-18 DEG C, cultivates in strict light protected environment 40-45 days；When mycelia will grow culture medium, spraying one layer of mass concentration on culture medium top layer is The gluconic acid zinc solution of 0.05-0.10%, fountain height is as the criterion just culture medium to be covered；Daytime is controlled Temperature 18-25 DEG C, is down to 12-18 DEG C night, gives illumination night, and illumination is no less than 4 hours, shines Degree 400-500Lx；

2), sporophore is cultivated: when culture medium top layer forms small sporophore, on culture medium top layer again Spraying the gluconic acid zinc solution that one layer of mass concentration is 0.05-0.1%, fountain height will be just will cultivate Base covers and is as the criterion；Adjusting temperature and be 18-20 DEG C, illumination every day is no less than 12 hours, illumination 1500-2000Lx, cultivates 8-12 days；

3), by step 2) in the cultivation temperature cultivated to the culture of the 8-12 days be adjusted to cultivate temperature Spending 18-25 DEG C, other condition is constant, carries out cellar culture, until results.

Compared with prior art, present invention have the advantage that

1, Cordyceps mycelium has extremely strong accumulation ability, under zinc ion is coerced, mycelia to zinc ion Internal cordycepin content can increase, the prior art application to zinc ion, and general employing is cultivating basigamy Adding zinc ion in side, advantage of this is that easy to operate, weak point is that mycelium is on initial rank Section just contacts zinc ion, the most slowly adapts to, and waits until to need the stage stimulating cordycepin to increase, zinc from The effect that son is coerced is bad.The cordycepin content of the sporophore obtained of non-zincification ionic stress is 0.05-0.10%；Use add zinc ion in the medium, sporophore cordycepin content up to 0.15-0.25%；The present invention by spraying zinc ion solution on specific stage culture medium top layer, permissible Bringing strong effect of coercing, sporophore cordycepin content is up to 0.25-0.35%.

2, prior art, uses zinc ion to coerce, and the concentration of zinc ion solution is higher, such as adopts With zinc acetate, zinc acetate accounts for the 2-9% of culture medium mass ratio, and the Fructus Vitis viniferae of the sprinkling of the employing of the present invention The solution concentration of saccharic acid zinc is 0.05-0.10%, and consumption is few, cost-effective, and zinc gluconate The glucose that fermentation needs can be provided.

3, the present invention is by using zinc ion to coerce cordycepin, in combination with the adjustment of illumination condition, Compared to existing technology, the incubation time of more than a week can be shortened.

Detailed description of the invention

With embodiment, the invention will be further described below, but the invention is not limited in that these are implemented Example.

Embodiment 1-10:

In order to verify the technique effect of the present invention, 2013 to 2014 years, use the technology of the present invention raw Producing the product of 10 batches, the method for each batch is as follows:

1), mycelia culture: finished bottle is put in 15-18 DEG C, cultivates in strict light protected environment 40-45 days；When mycelia will grow culture medium, spraying one layer of mass concentration on culture medium top layer is The gluconic acid zinc solution of 0.05-0.1%, fountain height is as the criterion just culture medium to be covered；Daytime is controlled Temperature 18-25 DEG C, is down to 12-18 DEG C night, gives illumination night, and illumination is no less than 4 hours, shines Degree 400-500Lx；

2), sporophore is cultivated: when culture medium top layer forms small sporophore, on culture medium top layer again Spraying the gluconic acid zinc solution that one layer of mass concentration is 0.05-0.1%, fountain height will be just will cultivate Base covers and is as the criterion；Adjusting temperature and be 18-20 DEG C, illumination every day is no less than 12 hours, illumination 1500-2000Lx, cultivates 8-12 days；

3), by step 2) in the cultivation temperature cultivated to the culture of the 8-12 days be adjusted to cultivate temperature Spending 18-25 DEG C, other condition is constant, carries out cellar culture, until results.

Each batch randomly draws one bottle therein, and after testing, result see table:

As can be seen from the above table, cordycepin content scope is 0.289-0.371%, and average content is 0. 335%.

Claims (1)

1. one kind is improved the method for cordycepin content in Cordyceps militaris (L.) Link.sporophore, it is characterised in that:

Comprise the steps:

1), mycelia culture: finished bottle is put in 15-18 DEG C, cultivates in strict light protected environment 40-45 days；When mycelia will grow culture medium, spraying one layer of mass concentration on culture medium top layer is The gluconic acid zinc solution of 0.05-0.10%, fountain height is as the criterion just culture medium to be covered；Daytime is controlled Temperature 18-25 DEG C, is down to 12-18 DEG C night, gives illumination night, and illumination is no less than 4 hours, shines Degree 400-500Lx；

2), sporophore is cultivated: when culture medium top layer forms small sporophore, on culture medium top layer again Spraying the gluconic acid zinc solution that one layer of mass concentration is 0.05-0.1%, fountain height will be just will cultivate Base covers and is as the criterion；Adjusting temperature and be 18-20 DEG C, illumination every day is no less than 12 hours, illumination 1500-2000Lx, cultivates 8-12 days；

3), by step 2) in the cultivation temperature cultivated to the culture of the 8-12 days be adjusted to cultivate temperature Spending 18-25 DEG C, other condition is constant, carries out cellar culture, until results.