CN105557307A - Method for cultivating lucid ganoderma by using traditional Chinese medicine - Google Patents
Method for cultivating lucid ganoderma by using traditional Chinese medicine Download PDFInfo
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- CN105557307A CN105557307A CN201511018819.1A CN201511018819A CN105557307A CN 105557307 A CN105557307 A CN 105557307A CN 201511018819 A CN201511018819 A CN 201511018819A CN 105557307 A CN105557307 A CN 105557307A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention provides a method for cultivating lucid ganoderma by using traditional Chinese medicines. The method comprises the following steps: culturing a strain, preparing a cultivation medium, culturing mycelium, and managing lucid ganoderma, wherein the step of preparing the cultivation medium comprises the following steps: mixing, crushing, bagging and sterilizing the following raw materials in parts by weight: 30-35 parts of weed tree sawdust, 10-20 parts of corncob, 5-10 parts of rice bran, 1-3 parts of grifola frondosa and 2-4 parts of gastrodia elata. According to the method, as gastrodia elata and grifola frondosa are added into a conventional cultivation medium, activity of alpha-glucophosphomutase can be improved, metabolism of lucid ganoderma cells can be improved to migrate to branches synthesized by ganoderan, the content of lucid ganoderma exopolysaccharides can be greatly increased, growth of lucid ganoderma can be also improved, and the time that a bag is full of mycelium can be greatly shortened.
Description
Technical field
The present invention relates to the technical field of cultivation of glossy ganoderma, be specifically related to the method utilizing Chinese medicine cultivation glossy ganoderma.
Background technology
Glossy ganoderma (Ganodermalucidum) is commonly called as polyporus lucidus, is a kind of medicinal fungi of preciousness.It has very high medical value, has health-care effect to human body, has prevention, treatment or inhibitory action to disease.The fruit body (or sclerotium) that traditional field acquisition or artificial cultivation are produced receives that wild resource is rare, the fruit body cultivation production cycle long (4 ~ May), produce the factors restrictions such as the conditions such as climate control and bioactive substance content is low, causes obstruction to a large amount of productions of glossy ganoderma.GL-B is the main effective chemical substance of glossy ganoderma, the homopolysaccharide that to be a class be formed by connecting with β-(1,3) and β-(Isosorbide-5-Nitrae) key or heteropolysaccharide macromolecular substances.Numerous research shows, GL-B has immunity moderation ability, antitumor, anti-oxidant, AntiHIV1 RT activity, the biologically active such as hypotensive.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of method utilizing Chinese medicine cultivation glossy ganoderma, and the method can significantly improve the content of polysaccharide in ganoderma lucidum fruitbody.
Technical scheme provided by the invention is a kind of method utilizing Chinese medicine cultivation glossy ganoderma, comprise Spawn incubation, medium preparing, cultural hypha, go out sesame management, described medium preparing is formed the raw material co-grinding of following weight portion, pack, sterilizing: weed tree sawdust 30 ~ 35 parts, corncob 10 ~ 20 parts, 5 ~ 10 parts, rice bran, grifola frondosus 1 ~ 3 part, 2 ~ 4 parts, rhizoma Gastrodiae.
Grifola frondosus (Grifolafrinosa) is a kind of medicine-food two-purpose bacterium of being rich in the active ingredients such as polysaccharide, vitamin, amino acid, protein, mineral salt.
Weed tree sawdust, corncob, rice bran are conventional culture medium raw materials, on this basis, also added grifola frondosus and rhizoma Gastrodiae, the interpolation of grifola frondosus and rhizoma Gastrodiae can improve the activity of alpha-phosphate glucose mutase, and alpha-phosphate glucose mutase take part in the synthesis of exocellular polysaccharide, alpha-phosphate glucose mutase activity strengthens, bootable G-6-P carries out to polysaccharide metabolic pathway of synthesizing direction, impel the metabolism of glossy ganoderma cell more towards the branch road migration of GL-B synthesis, thus greatly increase the content of glossy ganoderma exocellular polysaccharide.Moreover, grifola frondosus and rhizoma Gastrodiae composite after, can also promote that glossy ganoderma grows.
The moisture content of above-mentioned medium is 60 ~ 65%.
Above-mentioned cultural hypha medium is placed in gnotobasis inoculate bacterial classification, 22 ~ 25 DEG C, relative moisture is cultured to mycelia purseful under being 60 ~ 70% conditions.
Above-mentioned go out sesame management be the bacterium bag covering with mycelia is placed in 25 ~ 28 DEG C, relative moisture is 80 ~ 90%, intensity of illumination is be cultured to fruit body maturation under 1000 ~ 2000lux condition, can gather.
Compared with prior art, the present invention has following beneficial effect:
1) the present invention with the addition of rhizoma Gastrodiae and grifola frondosus in conventional medium, the activity of alpha-phosphate glucose mutase can be improved, impel the metabolism of glossy ganoderma cell more towards the branch road migration of GL-B synthesis, thus greatly increase the content of glossy ganoderma exocellular polysaccharide.
2) after rhizoma Gastrodiae and the composite interpolation of grifola frondosus, can also promote that glossy ganoderma grows, greatly shorten the mycelia purseful time.
Specific embodiment mode
The present invention is further elaborated for following specific embodiment, but not as a limitation of the invention.
Embodiment 1
1) bacterial classification is selected: adopt tissue isolation to obtain pure mother and plant, after a part of 75v% alcohol of wild or tame fresh Han Zhi fruit body of growing is carried out surface sterilization, the fritter of 3 ~ 5mm is cut into by aseptic manipulation, get about 5 pieces to be positioned on plating medium, or get 1 piece and be placed on test tube slant medium.Cultivate 3 ~ 4 days at 25 ~ 28 DEG C, when fritter organizes surrounding white mycelia to grow, select the mycelia that pure white nothing mixes and be transplanted on new slant medium, continue cultivation about 5 days, obtain lucid ganoderma stock culture, and then expand and be trained original seed and cultivated species.
2) medium preparing: the raw material by following weight portion: weed tree sawdust 30 parts, corncob 10 parts, 5 parts, rice bran, grifola frondosus 1 part and 2 parts, rhizoma Gastrodiae; By above-mentioned raw materials co-grinding, adding water to moisture content is 60%, can pack.Every bag of siccative 350 ~ 450g, Bag Material normal-pressure sterilization 10h, to be cooledly carry out aseptic inoculation to less than 30 DEG C.The bacterium bag inoculated is placed on indoor cultivation, maintains the temperature at 22 DEG C, relative moisture is 60%, when mycelium sends out full 2/3, move into and cultivate in canopy, indoor astigmatism, until mycelia purseful.
3) after mycelia sent out full by Ganoderma Lucidum bag, be placed in 25 DEG C, relative moisture is 80%, intensity of illumination is be cultured to fruit body maturation under 1000lux condition, can gather.
Reference examples 1
Identical with embodiment 1, only the raw material of medium is different, and the raw material that the raw material of the medium of this reference examples comprises following weight portion is made: weed tree sawdust 30 parts, corncob 10 parts and 5 parts, rice bran.
Embodiment 2
1) bacterial classification is selected: adopt tissue isolation to obtain pure mother and plant, after a part of 75v% alcohol of wild or tame fresh ganoderma lucidum sporocarp of growing is carried out surface sterilization, the fritter of 3 ~ 5mm is cut into by aseptic manipulation, get about 5 pieces to be positioned on plating medium, or get 1 piece and be placed on test tube slant medium.Cultivate 3 ~ 4 days at 25 ~ 28 DEG C, when fritter organizes surrounding white mycelia to grow, select the mycelia that pure white nothing mixes and be transplanted on new slant medium, continue cultivation about 5 days, obtain lucid ganoderma stock culture, and then expand and be trained original seed and cultivated species.
2) by the raw material of following weight portion: weed tree sawdust 35 parts, corncob 20 parts, 10 parts, rice bran, grifola frondosus 3 parts and 4 parts, rhizoma Gastrodiae; By above-mentioned raw materials co-grinding, adding water to moisture content is 65%, can pack.Every bag of siccative 350 ~ 450g, Bag Material normal-pressure sterilization 10h, to be cooledly carry out aseptic inoculation to less than 30 DEG C.The bacterium bag inoculated is placed on indoor cultivation, maintains the temperature at 25 DEG C, relative moisture is 70%, when mycelium sends out full 2/3, move into and cultivate in canopy, indoor astigmatism, until mycelia purseful.
3) after mycelia sent out full by Ganoderma Lucidum bag, be placed in 25 ~ 28 DEG C, relative moisture is 80 ~ 90%, intensity of illumination is be cultured to fruit body maturation under 1000 ~ 2000lux condition, can gather.
Reference examples 2
Identical with embodiment 2, only the raw material of medium is different, and the raw material that the raw material of the medium of this reference examples comprises following weight portion is made: weed tree sawdust 35 parts, corncob 20 parts and 10 parts, rice bran.
Embodiment 3
1) bacterial classification is selected: adopt tissue isolation to obtain pure mother and plant, after a part of 75v% alcohol of wild or tame new fresh and sweet sesame fruit body of growing is carried out surface sterilization, the fritter of 3 ~ 5mm is cut into by aseptic manipulation, get about 5 pieces to be positioned on plating medium, or get 1 piece and be placed on test tube slant medium.Cultivate 3 ~ 4 days at 25 ~ 28 DEG C, when fritter organizes surrounding white mycelia to grow, select the mycelia that pure white nothing mixes and be transplanted on new slant medium, continue cultivation about 5 days, obtain lucid ganoderma stock culture, and then expand and be trained original seed and cultivated species.
2) by the raw material of following weight portion: weed tree sawdust 32 parts, corncob 15 parts, 8 parts, rice bran, grifola frondosus 2 parts and 3 parts, rhizoma Gastrodiae; By above-mentioned raw materials co-grinding, add water to moisture 62%, can pack.Every bag of siccative 350 ~ 450g, Bag Material normal-pressure sterilization 10h, to be cooledly carry out aseptic inoculation to less than 30 DEG C.The bacterium bag inoculated is placed on indoor cultivation, maintains the temperature at 24 DEG C, relative moisture is 65%, when mycelium sends out full 2/3, move into and cultivate in canopy, indoor astigmatism, until mycelia purseful.
3) after mycelia sent out full by Ganoderma Lucidum bag, be placed in 26 DEG C, relative moisture is 85%, intensity of illumination is be cultured to fruit body maturation under 1500lux condition, can gather.
Reference examples 3
Identical with embodiment 3, only the raw material of medium is different, and the raw material that the raw material of the medium of this reference examples comprises following weight portion is made: weed tree sawdust 32 parts, corncob 15 parts and 8 parts, rice bran.
Reference examples 4
Identical with embodiment 3, only the raw material of medium is different, and the raw material that the raw material of the medium of this reference examples comprises following weight portion is made: weed tree sawdust 32 parts, corncob 15 parts, 8 parts, rice bran and grifola frondosus 2 parts.
Reference examples 5
Identical with embodiment 3, only the raw material of medium is different, and the raw material that the raw material of the medium of this reference examples comprises following weight portion is made: weed tree sawdust 32 parts, corncob 15 parts, 8 parts, rice bran and 3 parts, rhizoma Gastrodiae.
1, the mycelial growth table of comparisons of embodiment 1 ~ 3 and reference examples 1 ~ 3
2, the content table of comparisons of embodiment 1 ~ 3 and reference examples 1 ~ 3 separate sources GL-B
| Solid mycelia (%) | Fruit body (%) | |
| Embodiment 1 | 6.15 | 3.59 |
| Reference examples 1 | 3.35 | 1.28 |
| Embodiment 2 | 6.70 | 3.69 |
| Reference examples 2 | 3.11 | 1.15 |
| Embodiment 3 | 8.13 | 5.18 |
| Reference examples 3 | 4.03 | 1.58 |
| Reference examples 4 | 4.86 | 1.64 |
| Reference examples 5 | 4.92 | 1.89 |
Claims (4)
1. utilize the method for Chinese medicine cultivation glossy ganoderma, comprise Spawn incubation, medium preparing, cultural hypha, go out sesame management, it is characterized in that: described medium preparing is formed the raw material co-grinding of following weight portion, pack, sterilizing: weed tree sawdust 30 ~ 35 parts, corncob 10 ~ 20 parts, 5 ~ 10 parts, rice bran, grifola frondosus 1 ~ 3 part, 2 ~ 4 parts, rhizoma Gastrodiae.
2. the method utilizing Chinese medicine cultivation glossy ganoderma according to claim 1, is characterized in that: the moisture content of described medium is 60 ~ 65%.
3. the method utilizing Chinese medicine cultivation glossy ganoderma according to claim 1, is characterized in that: described cultural hypha medium is placed in gnotobasis inoculate bacterial classification, 22 ~ 25 DEG C, relative moisture is cultured to mycelia purseful under being 60 ~ 70% conditions.
4. the method utilizing Chinese medicine cultivation glossy ganoderma according to claim 1, it is characterized in that: described in go out sesame management be the bacterium bag covering with mycelia is placed in 25 ~ 28 DEG C, relative moisture is 80 ~ 90%, intensity of illumination is be cultured to fruit body maturation under 1000 ~ 2000lux condition, can gather.
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| CN201511018819.1A CN105557307A (en) | 2015-12-30 | 2015-12-30 | Method for cultivating lucid ganoderma by using traditional Chinese medicine |
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| CN201511018819.1A CN105557307A (en) | 2015-12-30 | 2015-12-30 | Method for cultivating lucid ganoderma by using traditional Chinese medicine |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107018802A (en) * | 2017-05-24 | 2017-08-08 | 贵州黔芝灵绿色农业科技发展有限公司 | A kind of ganoderma lucidum segment wood cultivated method |
| CN109661974A (en) * | 2019-02-20 | 2019-04-23 | 文山学院 | A method of white ginseng bacterium is cultivated using Radix Notoginseng undesirable root |
| CN111972209A (en) * | 2020-07-16 | 2020-11-24 | 山东仙农生物发展有限公司 | Cultivation method of semi-wild ganoderma lucidum |
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| CN1849869A (en) * | 2006-05-25 | 2006-10-25 | 开平市健之源食品有限公司 | Industrialized cultivation method for ganoderma lucidum |
| CN102187782A (en) * | 2010-03-10 | 2011-09-21 | 德阳市食用菌专家大院 | Method for culturing lucid ganoderma by using culture medium containing non-medicinal parts of Chinese medicinal materials |
| JP2013226130A (en) * | 2012-03-28 | 2013-11-07 | Unitika Ltd | New ganoderma neo-japonicum strain and method of artificial cultivation of the same |
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2015
- 2015-12-30 CN CN201511018819.1A patent/CN105557307A/en active Pending
Patent Citations (4)
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| CN1849869A (en) * | 2006-05-25 | 2006-10-25 | 开平市健之源食品有限公司 | Industrialized cultivation method for ganoderma lucidum |
| CN102187782A (en) * | 2010-03-10 | 2011-09-21 | 德阳市食用菌专家大院 | Method for culturing lucid ganoderma by using culture medium containing non-medicinal parts of Chinese medicinal materials |
| JP2013226130A (en) * | 2012-03-28 | 2013-11-07 | Unitika Ltd | New ganoderma neo-japonicum strain and method of artificial cultivation of the same |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107018802A (en) * | 2017-05-24 | 2017-08-08 | 贵州黔芝灵绿色农业科技发展有限公司 | A kind of ganoderma lucidum segment wood cultivated method |
| CN109661974A (en) * | 2019-02-20 | 2019-04-23 | 文山学院 | A method of white ginseng bacterium is cultivated using Radix Notoginseng undesirable root |
| CN111972209A (en) * | 2020-07-16 | 2020-11-24 | 山东仙农生物发展有限公司 | Cultivation method of semi-wild ganoderma lucidum |
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