CN103371044A - Selenium-enriched cordyceps flower production method - Google Patents
Selenium-enriched cordyceps flower production method Download PDFInfo
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- CN103371044A CN103371044A CN2012101315828A CN201210131582A CN103371044A CN 103371044 A CN103371044 A CN 103371044A CN 2012101315828 A CN2012101315828 A CN 2012101315828A CN 201210131582 A CN201210131582 A CN 201210131582A CN 103371044 A CN103371044 A CN 103371044A
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Abstract
The invention relates to the field of novel cordyceps production and cultivation technology, in particular to a selenium-enriched cordyceps flower production method which includes that sodium selenite is added when culture media for cordyceps production are prepared so that cultured cordyceps fruit bodies are led to be rich in selenium elements and the medical effect is greatly improved. By adopting the production method, values of cordyceps products are improved again, the selling price is improved, and benefit is considerable.
Description
Technical field
The present invention relates to a kind of Chinese caterpillar fungus and produce the new cultivation technology field, be specifically related to a kind of production method of selenium-rich cordyceps flower.
Technical background
Cordyceps flower is to pass through at present artificial cultivation a kind of rare traditional Chinese medicine and nourishing food out, is the substitute of natural cs.Through detecting, its medicinal efficacy and natural cs are basically identical, and the cordycepic acid that cordyceps flower is contained and cordycepin are more taller than natural Chinese caterpillar fungus.Show through modern medicine study: cordycepin is a kind of novel broad spectrum antibiotic, and unique effect is arranged in fields such as curing pulmonary tuberculosis.It also has anticancer, balance the body internal secretion and strengthens the effect of immune function of human body.In addition, scientific experiment proves that in the medical circle confessed seven large activity of fighting against senium compositions, Chinese caterpillar fungus has also been contained five large classes, is respectively polysaccharide, amino acid, polypeptide (protein), nucleic acid and vitamin, flavones and glycoside at present.In order further to improve the pharmacodynamic feature of Chinese caterpillar fungus, the scientific worker has also added in the middle of the process of producing Chinese caterpillar fungus---and selenium, selenium are the micronutrient elements of needed by human, and modern medicine shows that it is relevant with 40 various diseases to lack selenium.Mend selenium and can effectively reduce multiple angiocardiopathy, the incidence of disease of hepatonecrosis and cancer.Therefore, the selenium element is called as " kindling material of life, healthy bodyguard ".
Application number is " containing the abundant Chinese caterpillar fungus production method of selenium " of 201110099953.4, this invention is inoculated into cordyceps species (northern cordyceps species or aweto strain) in the specific culture liquid that has added sodium selenite exactly, under temperature control, obtain expanding numerous shaking flask kind through shaking flask, then it is inoculated on family's living silkworm in three to five ages, under the control of certain temperature and humidity, turn out and contain selenium abundant Chinese caterpillar fungus hypha and fruit body.This invention is to be inoculated on the living silkworm, and the silkworm application cost is high, and operating process is loaded down with trivial details, and operating efficiency is low.
Application number is 201010252973.6 " selenium-rich cordyceps culturing method ", this invention comprises following process step: (1) carries out the activation of bacterial classification at the PDA solid culture medium, (2) cultivation of liquid spawn, it is characterized in that it also carries out: the rich selenium of (3) fruit body is cultivated, it is by rice, selenate, glutelin hydrating solution form the rich seleno culture medium of fruit body, and selenate is a kind of in sodium selenate, potassium selenate, the ammonium selenate; The liquid spawn that the above-mentioned fermentation broth of access is turned out in the rich seleno culture medium of the fruit body of above-mentioned prescription requires to carry out to cultivate in 40～50 days according to hair tube reason.The Cordyceps militaris that this invention is cultivated, can increase substantially the Se content of fruiting bodies of cordyceps militaris, the fruit body Se content can reach 3.83-81.95 (μ g/g), and the growth potential of fruiting bodies of cordyceps militaris is strong, output is higher, bright in colour, thereby has improved nutrition and the medical value of Cordyceps militaris.But should the described technique more complicated of invention, the virtually higher cost of generation.
Summary of the invention
Purpose of the present invention is exactly to overcome the deficiency of above-mentioned technology and production method that brand-new a kind of selenium-rich cordyceps flower is provided.Specifically comprise: added sodium selenite when the preparation Chinese caterpillar fungus produces with culture matrix, made the cordyceps militaris sporocarp that cultivates be rich in the selenium element, medicinal and effect increases substantially.
The present invention is achieved through the following technical solutions:
A kind of production method of selenium-rich cordyceps flower may further comprise the steps in the technical scheme:
1, the substrate composed raw material of training and proportioning: rice 89%, dried silkworm chrysalis meal 5%, corn flour 3%, glucose 1%, yeast extract (powder) 0.8%, peptone 0.6%, content are 20% amino acid 0.3%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, water 130～160%.
2, produce with training matrix composition method: it is for subsequent use that rice, dried silkworm chrysalis meal and corn flour are mixed resulting mixture; Add successively above-mentioned glucose, yeast extract (powder), peptone, amino acid, potassium dihydrogen phosphate, magnesium sulfate and be stirred to the complete molten nutrient solution that forms of solids after heating water to boiling; Siccative weight per kilogram according to medium adds 20 milligrams of sodium selenites, and sodium selenite also adds in the nutrient solution and mixes thoroughly; Mixture divide install to the cultivation container in, then add 1.3～1.6 times nutrient solution by the dry weight of mixture; Then will cultivate and to produce after container is sterilized routinely with training matrix.
3, inoculation and cultivation, go out grass management: under gnotobasis, will cultivate routinely, spawn activity is strong, even, the free of contamination Chinese caterpillar fungus liquid spawn access of mycelia (ball) is produced with in the training matrix; Then will connect the cultivation container of planting and move to bacterial classification culturing room, and be cultured to routinely mycelia and eat up material and complete annesl; Move on to out at last the management of careless culturing room to going out grass.
The existing following advantage of the present invention:
1, simple, the easily enforcement of production method technique proposed by the invention.
2, the present invention is in implementation process, and at the bottom of the sodium selenite cost that adds, addition is few during production, so significantly promote the value of common cordyceps flower under the prerequisite that less increases cost.
3, by implementing the present invention, make all right cordyceps militaris sporocarp of original nutrition and effect be rich in again the selenium element, further improved thus nutrition and the medical value of Chinese caterpillar fungus.
4, by implementing the present invention, make the value of worm grass product that again lifting be arranged, selling price improves, and it is abundant to make a profit.
Embodiment
Below in conjunction with embodiment method of the present invention is further specified.
A kind of production method of selenium-rich cordyceps flower, embodiment is as follows:
1, the substrate composed raw material of training and proportioning: rice 89%, dried silkworm chrysalis meal 5%, corn flour 3%, glucose 1%, yeast extract (powder) 0.8%, peptone 0.6%, content are 20% amino acid 0.3%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, water 130～160%.
2, produce with training matrix composition method: it is for subsequent use that rice, dried silkworm chrysalis meal and corn flour are mixed resulting mixture; Add successively above-mentioned glucose, yeast extract (powder), peptone, amino acid, potassium dihydrogen phosphate, magnesium sulfate and be stirred to the complete molten nutrient solution that forms of solids after heating water to boiling; Siccative weight per kilogram according to medium adds 20 milligrams of sodium selenites, and sodium selenite also adds in the nutrient solution and mixes thoroughly; Mixture divide install to the cultivation container in, then add 1.3～1.6 times nutrient solution by the dry weight of mixture; Then will cultivate and to produce after container is sterilized routinely with training matrix.
3, inoculation and cultivation, go out grass management: under gnotobasis, will cultivate routinely, spawn activity is strong, even, the free of contamination Chinese caterpillar fungus liquid spawn access of mycelia (ball) is produced with in the training matrix; Then will connect the cultivation container of planting and move to bacterial classification culturing room, and be cultured to routinely mycelia and eat up material and complete annesl; Move on to out at last the management of careless culturing room to going out grass.
Claims (1)
1. the production method of selenium-rich cordyceps flower, its feature may further comprise the steps:
(1) the substrate composed raw material of training and proportioning: rice 89%, dried silkworm chrysalis meal 5%, corn flour 3%, glucose 1%, yeast extract (powder) 0.8%, peptone 0.6%, content are 20% amino acid 0.3%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, water 130～160%;
(2) produce with training matrix composition method: it is for subsequent use that rice, dried silkworm chrysalis meal and corn flour are mixed resulting mixture; Add successively above-mentioned glucose, yeast extract (powder), peptone, amino acid, potassium dihydrogen phosphate, magnesium sulfate and be stirred to the complete molten nutrient solution that forms of solids after heating water to boiling; Siccative weight per kilogram according to medium adds 20 milligrams of sodium selenites, and sodium selenite also adds in the nutrient solution and mixes thoroughly; Mixture divide install to the cultivation container in, then add 1.3～1.6 times nutrient solution by the dry weight of mixture; Then will cultivate and to produce after container is sterilized routinely with training matrix;
(3) inoculation and cultivation, go out grass management: under gnotobasis, will cultivate routinely, spawn activity is strong, even, the free of contamination Chinese caterpillar fungus liquid spawn access of mycelia (ball) is produced with in the training matrix; Then will connect the cultivation container of planting and move to bacterial classification culturing room, and be cultured to routinely mycelia and eat up material and complete annesl; Move on to out at last the management of careless culturing room to going out grass.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012101315828A CN103371044A (en) | 2012-04-29 | 2012-04-29 | Selenium-enriched cordyceps flower production method |
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| CN2012101315828A CN103371044A (en) | 2012-04-29 | 2012-04-29 | Selenium-enriched cordyceps flower production method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542484A (en) * | 2015-02-03 | 2015-04-29 | 何寒 | Breeding method of selenium-rich pork pig |
| CN105130632A (en) * | 2015-08-27 | 2015-12-09 | 马鞍山市安康菌业有限公司 | Efficient culture medium for herb residue-fermented cordycepin and preparation method therefor |
| CN105524843A (en) * | 2015-12-31 | 2016-04-27 | 浙江工业大学 | Production method of selenium-rich cordyceps flower |
| CN107211725A (en) * | 2017-05-12 | 2017-09-29 | 江苏绿洲园艺绿化有限公司 | A kind of production method of selenium-rich cordyceps flower |
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|---|---|---|---|---|
| CN1861782A (en) * | 2006-05-26 | 2006-11-15 | 李宏 | Artificial cultivating method of enriched selenium Cordyceps militaris and application of fruiting body |
| CN101050426A (en) * | 2007-03-28 | 2007-10-10 | 邹玉华 | Method for breeding north caterpillar fungus rich in selenium |
| CN101113415A (en) * | 2007-07-08 | 2008-01-30 | 曾建军 | Method for solid culture of Cordyceps sinensis edible mushroom |
| CN101897275A (en) * | 2010-08-13 | 2010-12-01 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
| CN102219601A (en) * | 2011-04-21 | 2011-10-19 | 金勇� | Formula of liquid culture medium of selenium-enriched cordyceps sinensis |
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2012
- 2012-04-29 CN CN2012101315828A patent/CN103371044A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1861782A (en) * | 2006-05-26 | 2006-11-15 | 李宏 | Artificial cultivating method of enriched selenium Cordyceps militaris and application of fruiting body |
| CN101050426A (en) * | 2007-03-28 | 2007-10-10 | 邹玉华 | Method for breeding north caterpillar fungus rich in selenium |
| CN101113415A (en) * | 2007-07-08 | 2008-01-30 | 曾建军 | Method for solid culture of Cordyceps sinensis edible mushroom |
| CN101897275A (en) * | 2010-08-13 | 2010-12-01 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
| CN102219601A (en) * | 2011-04-21 | 2011-10-19 | 金勇� | Formula of liquid culture medium of selenium-enriched cordyceps sinensis |
Non-Patent Citations (1)
| Title |
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| 凌宏通等: "蛹虫草的富硒栽培技术初步研究", 《中国食用菌》, vol. 25, no. 4, 31 December 2006 (2006-12-31), pages 52 - 53 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542484A (en) * | 2015-02-03 | 2015-04-29 | 何寒 | Breeding method of selenium-rich pork pig |
| CN105130632A (en) * | 2015-08-27 | 2015-12-09 | 马鞍山市安康菌业有限公司 | Efficient culture medium for herb residue-fermented cordycepin and preparation method therefor |
| CN105524843A (en) * | 2015-12-31 | 2016-04-27 | 浙江工业大学 | Production method of selenium-rich cordyceps flower |
| CN107211725A (en) * | 2017-05-12 | 2017-09-29 | 江苏绿洲园艺绿化有限公司 | A kind of production method of selenium-rich cordyceps flower |
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Application publication date: 20131030 |