CN104221717A - Method for increasing content of cordycepin in cordyceps militaris sporocarps - Google Patents

Abstract

The invention discloses a method for increasing the content of cordycepin in cordyceps militaris sporocarps. According to the method, zinc ion stress is applied to the cordycepin; meanwhile, a zinc gluconate solution is sprayed in a specific growth stage of the cordyceps militaris sporocarps by combining adjustment on an illumination condition, so that a strong stress effect can be achieved; the content of the cordycepin of the sporocarps can reach 0.25-0.35 percent. In addition, compared with the prior art, the method has the advantage that the cultivation time can be shortened by more than a week.

Description

A kind of method improving cordycepin content in fruiting bodies of cordyceps militaris

Technical field

The present invention relates to Artificial Planting Method of C. militarles field, particularly relate to a kind of Artificial Control condition of culture to improve the method for cordycepin content in fruiting bodies of cordyceps militaris.

Background technology

(Cordyceps mi1itaris, has another name called northern Chinese caterpillar Fungus to Cordyceps militaris, and fungal taxonomy belongs to Ascomycotina, gang pyrenomycetes, ergot Zoopagales, Clavicipitaceae, Cordyceps, is all the one of Cordyceps sinensis fungus with Cordyceps sinensis (Cordyceps sinensis).Cordyceps sinensis fungus is the general designation parasitizing the class entomogenous fungi compound that the underground fruit body of insect, spider or agglomerate capsule platymiscium is formed.Cordyceps militaris is the type sepecies of Cordyceps sinensis fungus, and main host plants is on the larva and pupa of Lepidoptera, coleoptera and more dipterous insects in the wild, and effect that the classic of TCM records it be " taste is sweet, property is flat; useful lung kidney; benefit marrow, hemostasis and phlegm ", basically identical with the function of Cordyceps sinensis.

Cordycepin is one of effective ingredient main in Cordyceps militaris, and cordycepin (cordycepin) has another name called 3 ｀ mono-desoxyadenossines, and be that Cuningham is separated a kind of adenosine class active substance obtained in nineteen fifty-one, molecular formula is C ₁₀h ₁₃n ₅0 ₃, molecular weight is 251.24, fusing point 230-231 DEG C.Cordycepin is one of effective ingredient main in Cordyceps militaris, has anti-bacteria and anti-virus, antitumor and antifatigue, anti-ageing, anti-inflammatory improve human immunological competence, suppress activity of monoamine oxidase, have higher medical value and pharmacological action widely.Clinically main application for the treatment of cancer, leukemia, expansion bronchus, significantly strengthen feritin, the anti-ageing effect of waiting for a long time.

In the past, due to Cordyceps militaris, output is rare in the wild, and its exploitation are not valued by the people.But from late nineteen nineties in last century, since its extensive artificial cultivation succeeds, its value of exploiting and utilizing is just more and more familiar with by people, and the research particularly for cordycepin gets more and more.The production application improving cordycepin content in artificial cultivation is mainly divided into and improves cordycepin content in liquid fermentation and culture thing, to improve in solid fermentation culture cordycepin content and improve cordycepin content three kinds of approach in fruit body, and wherein liquid fermentation and culture and solid fermentation are cultivated main to obtain cordycepin sterling for target.Improving cordycepin content in fruit body is then improve the commercial quality of fruiting bodies of cordyceps militaris for target.In the method improving cordycepin content, current existing technology mainly can be summarized as the following aspects: 1, improve cordycepin content by induction mutation of bacterium and seed selection; 2, by improving cordycepin content to medium Middle nutrition optimizing components; 3, cordycepin content is improved by adding precursor in the medium; 4, cordycepin content is improved by Fungal elicitor.At present, the worm grass product developed gets more and more, the market demand is also increasing, and the artificial cultivation of fruiting bodies of cordyceps militaris is just becoming the new industry that has very big economic worth, how to improve cordycepin content in fruiting bodies of cordyceps militaris and has good market prospects.

Summary of the invention

The object of this invention is to provide a kind of method improving cordycepin content in fruiting bodies of cordyceps militaris, it mainly by Artificial Control to improve cordycepin content, the cultural method of the fruiting bodies of cordyceps militaris used in the method and prior art and the method improving cordycepin content can use simultaneously.

The present invention is achieved by the following technical solution:

1), cultural hypha: finished bottle is put in 15-18 DEG C, cultivates 40-45 days in strict light protected environment; When mycelia will grow medium, spray the gluconic acid zinc solution that one deck mass concentration is 0.05-0.10% on medium top layer, fountain height is as the criterion just to be covered by medium; Daytime temperature control 18-25 DEG C, night is down to 12-18 DEG C, and night gives illumination, and illumination is no less than 4 hours, illumination 400-500Lx;

2), fruit body is cultivated: when medium top layer forms small fruit body, again spray the gluconic acid zinc solution that one deck mass concentration is 0.05-0.1% on medium top layer, fountain height is as the criterion just to be covered by medium; Adjustment temperature is 18-20 DEG C, and illumination every day is no less than 12 hours, illumination 1500-2000Lx, cultivates 8-12 days;

3), by step 2) in the cultivation temperature being cultured to the culture of 8-12 days be adjusted to cultivation temperature 18-25 DEG C, other condition is constant, carries out cellar culture, until results.

Compared with prior art, tool of the present invention has the following advantages:

1, cordyceps mycelia has extremely strong accumulation ability to zinc ion, under zinc ion is coerced, in mycelium, cordycepin content can increase, prior art is to the application of zinc ion, and general employing adds zinc ion in culture medium prescription, and the benefit done like this is easy to operate, weak point is that mycelium just contacts zinc ion in the starting stage, slowly adapt to, by the time need the stage stimulating cordycepin to increase, the effect that zinc ion is coerced is bad.The cordycepin content of the fruit body obtained of non-zincification ionic stress is 0.05-0.10%; Adopt and add zinc ion in the medium, fruit body cordycepin content can reach 0.15-0.25%; The present invention is by spraying zinc ion solution on specific stage medium top layer, and can bring and strong coerce effect, fruit body cordycepin content can reach 0.25-0.35%.

2, prior art, employing zinc ion is coerced, the concentration of zinc ion solution is higher, such as adopt zinc acetate, zinc acetate accounts for the 2-9% of medium mass ratio, and the solution concentration of the zinc gluconate of the sprinkling of employing of the present invention is 0.05-0.10%, and consumption is few, cost-saving, and the glucose that zinc gluconate can provide fermentation to need.

3, the present invention is by adopting zinc ion to coerce to cordycepin, simultaneously in conjunction with the adjustment of illumination condition, compared to existing technology, can shorten the incubation time of more than a week.

Embodiment

With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.

Embodiment 1-10:

In order to verify technique effect of the present invention, 2013 to 2014, use the technology of the present invention to produce the product of 10 batches, the method for each batch was as follows:

1), cultural hypha: finished bottle is put in 15-18 DEG C, cultivates 40-45 days in strict light protected environment; When mycelia will grow medium, spray the gluconic acid zinc solution that one deck mass concentration is 0.05-0.1% on medium top layer, fountain height is as the criterion just to be covered by medium; Daytime temperature control 18-25 DEG C, night is down to 12-18 DEG C, and night gives illumination, and illumination is no less than 4 hours, illumination 400-500Lx;

2), fruit body is cultivated: when medium top layer forms small fruit body, again spray the gluconic acid zinc solution that one deck mass concentration is 0.05-0.1% on medium top layer, fountain height is as the criterion just to be covered by medium; Adjustment temperature is 18-20 DEG C, and illumination every day is no less than 12 hours, illumination 1500-2000Lx, cultivates 8-12 days;

3), by step 2) in the cultivation temperature being cultured to the culture of 8-12 days be adjusted to cultivation temperature 18-25 DEG C, other condition is constant, carries out cellar culture, until results.

Each batch of a bottle of randomly drawing wherein, after testing, the results are shown in following table:

As can be seen from the above table, cordycepin content scope is 0.289-0.371%, and average content is 0.335%.

Claims (1)

1. improve a method for cordycepin content in fruiting bodies of cordyceps militaris, it is characterized in that:

Comprise the steps:

1), cultural hypha: finished bottle is put in 15-18 DEG C, cultivates 40-45 days in strict light protected environment; When mycelia will grow medium, spray the gluconic acid zinc solution that one deck mass concentration is 0.05-0.10% on medium top layer, fountain height is as the criterion just to be covered by medium; Daytime temperature control 18-25 DEG C, night is down to 12-18 DEG C, and night gives illumination, and illumination is no less than 4 hours, illumination 400-500Lx;

2), fruit body is cultivated: when medium top layer forms small fruit body, again spray the gluconic acid zinc solution that one deck mass concentration is 0.05-0.1% on medium top layer, fountain height is as the criterion just to be covered by medium; Adjustment temperature is 18-20 DEG C, and illumination every day is no less than 12 hours, illumination 1500-2000Lx, cultivates 8-12 days;

3), by step 2) in the cultivation temperature being cultured to the culture of 8-12 days be adjusted to cultivation temperature 18-25 DEG C, other condition is constant, carries out cellar culture, until results.