CN104025891A - Suberect spatholobus stem cutting propagation method - Google Patents
Suberect spatholobus stem cutting propagation method Download PDFInfo
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- CN104025891A CN104025891A CN201410300435.8A CN201410300435A CN104025891A CN 104025891 A CN104025891 A CN 104025891A CN 201410300435 A CN201410300435 A CN 201410300435A CN 104025891 A CN104025891 A CN 104025891A
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Abstract
The invention discloses a suberect spatholobus stem cutting propagation method and belongs to the technical field of Chinese herbal medicine cultivation. The method includes branching collecting, branch shearing, plant hormones soaking, sand bed preparing, cutting propagating, watering, shading steps. The method has the advantages that materials can be prepared conveniently, procedures are simple, the rooting rate of the suberect spatholobus stem ranges from 80% to 85%, the genetic gene is not mutated, after transplanting, fast, stable and high yield can be achieved, and sufficient high-quality seedlings can be supplied for large-area artificial cultivation.
Description
Technical field
The present invention relates to Chinese medicinal herbs culture technique field, be specifically related to a kind of method that reticulate millettia is grown seedlings, the especially cuttage and seedling culture method of reticulate millettia, belongs to traditional Chinese medicine culture technique field.
Background technology
Reticulate millettia formal name used at school is Spatholobus suberectus Dunn, belongs to Dicotyledoneae pulse family Kadsura Schisandra sphenanthera Rehd.et WilS kind, is mainly distributed in Yunnan and Southwestern Kwangsi, China, grows in 1800 meters of following woodss of height above sea level.
The medicinal part of reticulate millettia is dry root and rhizome, cure mainly rheumatism tibialgia, irregular menstruation, the deficiency of blood are sallow, paralysis and numbness, arthralgia due to wind-dampness etc., for China's traditional Chinese medicine material, be the raw material of a lot of clinical applications, market demand is huge, reticulate millettia impoverishment tolerant, strong adaptability, reaches tens of rice sometimes, is also the desirable liane kind of urban afforestation and stony desertification, desertification treatment.
Adopted due to ecological impact and artificial unordered unrest in recent years, and do not focus on the protection of resource, and made wild reticulate millettia quantity fewer and feweri, resource reduces day by day.For protection with develop this good herbal variety, for satisfying the demand, people have to develop artificial cultivation technique, and carry out reticulate millettia artificial cultivation, first will solve the reticulate millettia problem of growing seedlings.But now people to carry out that reticulate millettia grows seedlings be all to utilize the seed of reticulate millettia to cultivate, be to carry out sexual propagation, its hereditary feature can changes by relative parent, occurs that active ingredient reduces, drug effect reduction, and poky defect.
Summary of the invention
goal of the invention: the object of the invention is for problems of the prior art, provide a kind of simple, keep wild active constituents in vine stem of Spatholobus suberectus, the fast asexual multiplication seedling method of growth after transplanting.
technical scheme: a kind of reticulate millettia cuttage and seedling culture method of the present invention, specifically comprises the following steps:
1. adopt annual stalwartness, reticulate millettia stem branch without damage by disease and insect, be cut into long 20～30 centimetres, with the cutting of 2～3 joints;
2. by naa (NAA) or the plant hormone immersion treatment of indolebutyric acid (IBA) 6～10 hours, the concentration of plant hormone was 160mg/L～190mg/L;
3. on whole good husky bed, open 18 centimetres of dark ditches with scuppit, the cutting of handling well is tiltedly swung in ditch by the spacing in the rows of 6 centimetres, cutting and Shamian Island are 40 degree, cover sand, and it is 2/3 of its length that cutting enters the husky degree of depth, and line-spacing is 13 centimetres of left and right;
4. after inserting every day drench water 2 times, make water supply in media remain on 55%～60%;
5. shade with sunshade net scaffolding, avoid direct sunlight, temperature of shed is 26 DEG C～30 DEG C;
6. cutting is thrown off sunshade net after germinateing and taking root successively, and cutting is cultivated and can be transplanted for about 60 days.
beneficial effect: compared with prior art, its beneficial effect is in the present invention:
Reticulate millettia seedling-cultivating method of the present invention, draws materials conveniently, and operation is simple, and reticulate millettia goes out root rate and reaches 80%～85%, and gene do not make a variation, and after transplanting, more reaches fast-growing, stable and high yields.
embodiment
Below in conjunction with the concrete example of implementing, technical solution of the present invention is elaborated.
Embodiment 1: a kind of reticulate millettia cuttage and seedling culture, its concrete steps are as follows:
1. adopt annual stalwartness, reticulate millettia stem branch without damage by disease and insect, be cut into long 25 centimetres, with the cutting of 2～3 joints;
2. by the plant hormone immersion treatment 10 hours of indolebutyric acid (IBA), the concentration of plant hormone is 160mg/L;
3. on whole good husky bed, open 18 centimetres of dark ditches with scuppit, the cutting of handling well is tiltedly swung in ditch by the spacing in the rows of 6 centimetres, cutting and Shamian Island are 40 degree, cover sand, and it is 2/3 of its length that cutting enters the husky degree of depth, 13 centimetres of line-spacings;
4. after inserting every day drench water 2 times, make water supply in media remain on 55%～60%;
5. shade with sunshade net scaffolding, avoid direct sunlight, temperature of shed is 26 DEG C～28 DEG C;
6. cutting is thrown off sunshade net after germinateing and taking root successively, and cutting is cultivated and transplanted afterwards for 60 days.
This cuttage and seedling culture rhizome goes out root rate and reaches 82.8%.
Embodiment 2: a kind of reticulate millettia cuttage and seedling culture, its concrete steps are as follows:
1. adopt annual stalwartness, reticulate millettia stem branch without damage by disease and insect, be cut into long 25 centimetres, with the cutting of 2～3 joints;
2. by the plant hormone immersion treatment 10 hours of indolebutyric acid (IBA), the concentration of plant hormone is 170mg/L;
3. on whole good husky bed, open 18 centimetres of dark ditches with scuppit, the cutting of handling well is tiltedly swung in ditch by the spacing in the rows of 6 centimetres, cutting and Shamian Island are 40 degree, cover sand, and it is 2/3 of its length that cutting enters the husky degree of depth, and line-spacing is 13 centimetres;
4. after inserting every day drench water 2 times, make water supply in media remain on 55%～60%;
5. shade with sunshade net scaffolding, avoid direct sunlight, temperature of shed is 28 DEG C～30 DEG C;
6. cutting is thrown off sunshade net after germinateing and taking root successively, and cutting is cultivated and transplanted afterwards for 60 days.
This cuttage and seedling culture rhizome goes out root rate and reaches 85.3%.
Embodiment 3: a kind of reticulate millettia cuttage and seedling culture, its concrete steps are as follows:
1. adopt annual stalwartness, reticulate millettia stem branch without damage by disease and insect, be cut into long 25 centimetres, with the cutting of 2～3 joints;
2. by the plant hormone immersion treatment 10 hours of indolebutyric acid (IBA), the concentration of plant hormone is 190mg/L;
3. on whole good husky bed, open 18 centimetres of dark ditches with scuppit, the cutting of handling well is tiltedly swung in ditch by the spacing in the rows of 6 centimetres, cutting and Shamian Island are 40 degree, cover sand, and it is 2/3 of its length that cutting enters the husky degree of depth, and line-spacing is 13 centimetres;
4. after inserting every day drench water 2 times, make water supply in media remain on 55%～60%;
5. shade with sunshade net scaffolding, avoid direct sunlight, temperature of shed is 26 DEG C～28 DEG C;
6. cutting is thrown off sunshade net after germinateing and taking root successively, and cutting is cultivated and transplanted afterwards for 60 days.
This cuttage and seedling culture rhizome goes out root rate and reaches 79.4%.
Claims (4)
1. a reticulate millettia cuttage and seedling culture method, is characterized in that comprising the following steps:
(1) adopt annual stalwartness, reticulate millettia stem branch without damage by disease and insect, be cut into long 25 centimetres, with the cutting of 2～3 joints;
(2) use plant hormone immersion treatment;
(3) on whole good husky bed, open 18 centimetres of dark ditches with scuppit, the cutting of handling well is tiltedly swung in ditch by the spacing in the rows of 6 centimetres, cutting and Shamian Island are 40 degree, cover sand, and it is 2/3 of its length that cutting enters the husky degree of depth, and line-spacing is 13 centimetres;
(4) insert after every day drench water 2 times, make water supply in media remain on 55%～60%;
(5) shade with sunshade net scaffolding, avoid direct sunlight, temperature of shed is 26 DEG C～30 DEG C;
(6) cutting is thrown off sunshade net after germinateing and taking root successively, and cutting is cultivated and transplanted afterwards for 60 days.
2. reticulate millettia cuttage and seedling culture method according to claim 1, is characterized in that the plant hormone of described step (2) is indolebutyric acid (IBA).
3. reticulate millettia cuttage and seedling culture method according to claim 2, is characterized in that described indolebutyric acid (IBA) concentration is 160mg/L～190mg/L, is preferably 170mg/L.
4. reticulate millettia cuttage and seedling culture method according to claim 1, is characterized in that the time of described step (2) immersion treatment is 6～10 hours.
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| CN201410300435.8A CN104025891A (en) | 2014-06-30 | 2014-06-30 | Suberect spatholobus stem cutting propagation method |
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| CN201410300435.8A CN104025891A (en) | 2014-06-30 | 2014-06-30 | Suberect spatholobus stem cutting propagation method |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105009909A (en) * | 2015-07-30 | 2015-11-04 | 区燕丽 | Caulis spatholobi planting method |
| CN105613195A (en) * | 2016-03-14 | 2016-06-01 | 黄振忠 | Euonymus fortunei cutting seedling method |
| CN105766326A (en) * | 2016-03-25 | 2016-07-20 | 黄振忠 | Method for culturing seedlings of euonymus fortune through nutrition cup |
| CN105850270A (en) * | 2016-04-14 | 2016-08-17 | 梁英强 | Method for breaking caulis spatholobi seed dormancy |
| CN106171802A (en) * | 2016-08-31 | 2016-12-07 | 株洲千金药业股份有限公司 | A kind of breeding method of the wild seedling of Caulis Spatholobi |
| CN106818491A (en) * | 2017-04-08 | 2017-06-13 | 广西科技大学鹿山学院 | A kind of culture medium for improving reticulate millettia tissue-cultured seedling flavone compound and polysaccharide |
| CN107087483A (en) * | 2017-03-22 | 2017-08-25 | 陈宣强 | A kind of cuttage breeding method of reticulate millettia |
| CN108307822A (en) * | 2018-01-05 | 2018-07-24 | 莫玉明 | A kind of raspberry cuttage and seedling culture method |
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| CN102138396A (en) * | 2010-11-29 | 2011-08-03 | 天津滨海国际花卉科技园区股份有限公司 | Cuttage method for Amaranthus tricolor |
| CN103430753A (en) * | 2013-09-04 | 2013-12-11 | 黄振忠 | Cutting seedling method of subprostrate sophora |
| CN103503669A (en) * | 2012-06-29 | 2014-01-15 | 陈伟 | Short shoot cutting breeding method of golden guanyin tea seedling |
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| CN102138396A (en) * | 2010-11-29 | 2011-08-03 | 天津滨海国际花卉科技园区股份有限公司 | Cuttage method for Amaranthus tricolor |
| CN103503669A (en) * | 2012-06-29 | 2014-01-15 | 陈伟 | Short shoot cutting breeding method of golden guanyin tea seedling |
| CN103430753A (en) * | 2013-09-04 | 2013-12-11 | 黄振忠 | Cutting seedling method of subprostrate sophora |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105009909A (en) * | 2015-07-30 | 2015-11-04 | 区燕丽 | Caulis spatholobi planting method |
| CN105613195A (en) * | 2016-03-14 | 2016-06-01 | 黄振忠 | Euonymus fortunei cutting seedling method |
| CN105766326A (en) * | 2016-03-25 | 2016-07-20 | 黄振忠 | Method for culturing seedlings of euonymus fortune through nutrition cup |
| CN105850270A (en) * | 2016-04-14 | 2016-08-17 | 梁英强 | Method for breaking caulis spatholobi seed dormancy |
| CN106171802A (en) * | 2016-08-31 | 2016-12-07 | 株洲千金药业股份有限公司 | A kind of breeding method of the wild seedling of Caulis Spatholobi |
| CN107087483A (en) * | 2017-03-22 | 2017-08-25 | 陈宣强 | A kind of cuttage breeding method of reticulate millettia |
| CN106818491A (en) * | 2017-04-08 | 2017-06-13 | 广西科技大学鹿山学院 | A kind of culture medium for improving reticulate millettia tissue-cultured seedling flavone compound and polysaccharide |
| CN108307822A (en) * | 2018-01-05 | 2018-07-24 | 莫玉明 | A kind of raspberry cuttage and seedling culture method |
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Application publication date: 20140910 |