CN115868581A - Beef cattle fermented feed and preparation method thereof - Google Patents

Beef cattle fermented feed and preparation method thereof Download PDF

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Publication number
CN115868581A
CN115868581A CN202310021039.0A CN202310021039A CN115868581A CN 115868581 A CN115868581 A CN 115868581A CN 202310021039 A CN202310021039 A CN 202310021039A CN 115868581 A CN115868581 A CN 115868581A
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parts
mixed
hundred million
beef cattle
feed
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张相伦
梁萌
赵红波
刘晓牧
李川皓
盛清凯
杨赵军
陈祥兴
李庆雷
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Institute Animal Science and Veterinary Medicine of Shandong AAS
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Institute Animal Science and Veterinary Medicine of Shandong AAS
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Priority to ZA2023/06937A priority patent/ZA202306937B/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention relates to the technical field of feed preparation, in particular to a beef cattle fermented feed and a preparation method thereof. The invention provides a beef cattle fermented feed, which comprises the following components: 20-30 parts of brewer's grain, 20-30 parts of puffed soybean, 20-28 parts of bean curd residue, 18-22 parts of mixed grass meal, 3-7 parts of corn oil, 3-7 parts of molasses, 3-5 parts of salt, 2-4 parts of magnesium sulfate, 2-4 parts of sodium bicarbonate, 4-8 parts of mixed vitamin, 4-8 parts of mixed mineral, 3-7 parts of mixed microbial inoculum and 3-7 parts of fermentation microbial inoculum. The beef cattle fermented feed can increase rumen peristalsis and running capacity of beef cattle, promote normal rumination, improve digestion level and improve dry matter feed intake.

Description

Beef cattle fermented feed and preparation method thereof
Technical Field
The invention relates to the technical field of feed preparation, in particular to a beef cattle fermented feed and a preparation method thereof.
Background
The beef cattle, namely beef cattle, is a cattle mainly used for producing beef, and has the characteristics of quick weight increment, utilization of straw byproducts, good meat production performance, good meat quality and taste and the like. The beef cattle breeding industry has wide development prospect.
During the feeding process of beef cattle, if a large amount of concentrated feed is fed to rapidly fatten beef cattle, and a short-term profit is pursued, growth-promoting drugs and additives are illegally added, the additives destroy rumen microorganisms, although the beef cattle can be rapidly fattened in a short term, the rumen microorganisms are gradually destroyed and die, rumen food is easily formed before slaughtering, acidosis is caused, the morbidity of the beef cattle (especially diseases of a digestive system and a metabolic system) is greatly increased, diarrhea and growth stop are caused, and harmful bacteria are propagated to cause diseases and then easily die; meanwhile, due to the digestive dysfunction of the beef cattle, the feed intake of the beef cattle is greatly reduced or the beef cattle do not eat the feed, and the diarrhea is seriously caused, so that the beef cattle are in a disease and sub-health state before slaughtering, and the beef quality is also seriously influenced.
The raw materials of the cattle feed are generally added with nutrient substances such as crude fiber, and microorganisms in the rumen of ruminants have limited ability of decomposing the crude fiber and poor digestion and absorption, so that the conversion rate of the cattle feed is low. In order to improve the conversion rate of the cattle feed, strains are added into the feed, and the raw materials of the feed are subjected to primary fermentation to form fermented animal feed, so that the conversion rate of the animal feed can be improved. But also has the technical problems of single selection of fermentation strains, complex strain sources, low nutrient level, poor feed fermentation effect and influence on rumen fermentation of beef cattle.
Therefore, the technical problem that needs to be solved by the technical personnel in the field is how to provide a fermented feed which can promote the growth of rumen microorganisms of beef cattle and improve the health state and meat quality of beef cattle so as to solve the technical problems that the fermented feed in the prior art disturbs the rumen fermentation of beef cattle, and the fermentation strains are single.
Disclosure of Invention
The invention aims to provide a beef cattle fermented feed and a preparation method thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a beef cattle fermented feed which is prepared from the following components in parts by mass: 20-30 parts of brewer's grain, 20-30 parts of puffed soybean, 20-28 parts of bean curd residue, 18-22 parts of mixed grass meal, 3-7 parts of corn oil, 3-7 parts of molasses, 3-5 parts of salt, 2-4 parts of magnesium sulfate, 2-4 parts of sodium bicarbonate, 4-8 parts of mixed vitamin, 4-8 parts of mixed mineral, 3-7 parts of mixed microbial inoculum and 3-7 parts of fermentation microbial inoculum.
Preferably, the mixed grass powder comprises: pennisetum hydridum powder, peanut seedling leaf powder, straw powder and alfalfa powder, wherein the mass ratio of pennisetum hydridum powder to peanut seedling leaf powder to alfalfa powder is 3-8: 5 to 9:12 to 16:20 to 30.
Preferably, the mixed microbial inoculum is lactobacillus casei, lactobacillus acidophilus, pediococcus acidilactici, saccharomyces cerevisiae, bacillus aceticus, aspergillus oryzae and aspergillus niger; the viable count of lactobacillus casei in the mixed microbial inoculum is 8-12 hundred million/ml, the viable count of acidophilic lactobacillus is 8-12 hundred million/ml, the viable count of pediococcus acidilactici is 6-10 hundred million/ml, the viable count of saccharomyces cerevisiae is 3-5 hundred million/ml, the viable count of acetobacter is 3-5 hundred million/ml, the viable count of aspergillus oryzae is 2-4 hundred million/ml, and the viable count of aspergillus niger is 2-4 hundred million/ml.
Preferably, the fermentation inoculum is: bacillus amyloliquefaciens, candida tropicalis, trichoderma koningii, trichoderma reesei, rhodopseudomonas palustris, and Bacillus coagulans; the number of viable bacteria of bacillus amyloliquefaciens is 9-11 hundred million/ml, the number of viable bacteria of candida tropicalis is 8-10 hundred million/ml, the number of viable bacteria of trichoderma koningii is 6-8 hundred million/ml, the number of viable bacteria of trichoderma reesei is 5-7 hundred million/ml, the number of viable bacteria of rhodopseudomonas palustris is 2-6 hundred million/ml, and the number of viable bacteria of bacillus coagulans is 2-4 hundred million/ml.
Preferably, the dosage of the mixed vitamins in the feed is as follows: 9000-11000 IU/kg of vitamin A, 32400-3600 IU/kg of vitamin D, 34-6 mg/kg of vitamin K, 11-3 mg/kg of vitamin B, 27-9 mg/kg of vitamin B, 14-6 mg/kg of vitamin E, 35-45 mg/kg of nicotinic acid and 4-6 mg/kg of folic acid.
Preferably, the dosage of the mixed mineral in the feed is 900-1100 mg/kg of copper, 7500-8500 mg/kg of iron, 4000-6000 mg/kg of zinc, 4000-6000 mg/kg of manganese, 40-60 mg/kg of iodine, 30-40 mg/kg of chromium and 10-30 mg/kg of cobalt.
The invention also provides a preparation method of the beef cattle fermented feed, which comprises the following steps:
(1) Mixing the mixed grass meal with a fermentation microbial inoculum, adjusting the water content, and fermenting to obtain a material 1;
(2) Mixing and fermenting the material 1, the brewer's grains, the puffed soybeans, the bean curd residues and the mixed microbial inoculum to obtain a material 2;
(3) Mixing the material 2, corn oil, molasses, salt, magnesium sulfate, sodium bicarbonate, mixed vitamins and mixed minerals, and drying.
Preferably, the water content is adjusted to 55-65% in the step (1); and (3) drying until the water content is less than or equal to 14%.
Preferably, the fermentation time in the step (1) is 7-11 d; the fermentation time in the step (2) is 30-40 h.
Compared with the prior art, the invention has the following beneficial effects:
1. the fermented feed for beef cattle can increase rumen peristalsis and running capacity of beef cattle, promote normal rumination, and improve dry matter feed intake and digestion level. And the probiotics are added, so that the diversity of rumen flora can be improved, the propagation of harmful microorganisms is inhibited, and gastrointestinal diseases are prevented.
2. The compound vitamins and mineral substances are added into the product, so that the digestibility of nutrient substances in small intestine sections of beef cattle can be improved. Fat-soluble vitamins such as vitamin A and vitamin D3 can enhance calcium and phosphorus absorption, promote skeleton development, enlarge beef cattle skeleton, and promote growth. The B vitamins are generally considered not to be added in the beef cattle feed, but due to the large intake of concentrated feed, the B vitamins generated by rumen fermentation are not enough to meet the needs of the organism, so that the additional addition of the nicotinamide and the like can promote the digestion and absorption of nutrient substances, and the daily gain is fast.
3. The beef cattle feed is prepared by a double fermentation method, firstly, the mixed grass meal and a leavening agent are mixed and fermented, probiotics such as bacillus amyloliquefaciens and the like contained in the mixed grass meal can decompose macromolecular substances such as cellulose and the like in the grass meal into micromolecular substances so as to be beneficial to the digestion and absorption of beef cattle, then, the second fermentation is carried out, the protein content in the feed can be improved, the detoxification effect is achieved, and the probiotics contained in the mixed microbial inoculum is also beneficial to the rumen fermentation process of the beef cattle.
4. The invention adopts the brewer's grains and the bean curd residues to replace the corn in the traditional feed, and after the brewer's grains and the bean curd residues are fermented by beneficial bacteria, the nutrient components of the brewer's grains and the bean curd residues can completely meet the daily requirement of beef cattle, and the cost of the feed is reduced.
5. Among the mixed grass meal added in the invention, pennisetum hydridum meal, peanut vine leaf meal, straw meal and alfalfa meal have wide sources and low cost, and contain rich nutrient components such as dry substances, crude protein, crude fat and the like.
Detailed Description
The invention provides a beef cattle fermented feed which is prepared from the following components in parts by mass: 20-30 parts of brewer's grain, 20-30 parts of puffed soybean, 20-28 parts of soybean curb residue, 18-22 parts of mixed grass meal, 3-7 parts of corn oil, 3-7 parts of molasses, 3-5 parts of salt, 2-4 parts of magnesium sulfate, 2-4 parts of sodium bicarbonate, 4-8 parts of mixed vitamin, 4-8 parts of mixed mineral substance, 3-7 parts of mixed microbial inoculum and 3-7 parts of fermentation microbial inoculum; preferably 22 to 28 parts of brewer's grain, 22 to 28 parts of puffed soybean, 21 to 27 parts of bean curd residue, 19 to 21 parts of mixed grass meal, 4 to 6 parts of corn oil, 4 to 6 parts of molasses, 4 parts of salt, 3 parts of magnesium sulfate, 3 parts of sodium bicarbonate, 5 to 7 parts of mixed vitamin, 5 to 7 parts of mixed mineral, 4 to 6 parts of mixed microbial inoculum and 4 to 6 parts of fermentation microbial inoculum; more preferably 25 parts of brewer's grain, 25 parts of puffed soybean, 24 parts of bean curd residue, 20 parts of mixed grass meal, 5 parts of corn oil, 5 parts of molasses, 4 parts of salt, 3 parts of magnesium sulfate, 3 parts of sodium bicarbonate, 6 parts of mixed vitamin, 6 parts of mixed mineral, 6 parts of mixed microbial inoculum and 6 parts of fermentation microbial inoculum.
In the present invention, the mixed grass powder includes: pennisetum hydridum powder, peanut vine leaf powder, straw powder and alfalfa powder, wherein the mass ratio of the pennisetum hydridum powder to the peanut vine leaf powder to the alfalfa powder is 3-8: 5 to 9:12 to 16:20 to 30 percent; preferably 6:7:14:25.
in the invention, the mixed microbial inoculum is lactobacillus casei, lactobacillus acidophilus, pediococcus acidilactici, saccharomyces cerevisiae, bacillus aceticus, aspergillus oryzae and aspergillus niger; the viable count of lactobacillus casei in the mixed microbial inoculum is 8-12 hundred million/ml, the viable count of acidophilic lactobacillus is 8-12 hundred million/ml, the viable count of pediococcus acidilactici is 6-10 hundred million/ml, the viable count of saccharomyces cerevisiae is 3-5 hundred million/ml, the viable count of acetobacter is 3-5 hundred million/ml, the viable count of aspergillus oryzae is 2-4 hundred million/ml, and the viable count of aspergillus niger is 2-4 hundred million/ml; preferably, the viable count of Lactobacillus casei is 10 hundred million/ml, the viable count of Lactobacillus acidophilus is 10 hundred million/ml, the viable count of Pediococcus acidilactici is 8 hundred million/ml, the viable count of Saccharomyces cerevisiae is 4 hundred million/ml, the viable count of Acetobacter aceti is 4 hundred million/ml, the viable count of Aspergillus oryzae is 3 hundred million/ml, and the viable count of Aspergillus niger is 3 hundred million/ml.
In the invention, the fermentation inoculum is: bacillus amyloliquefaciens, candida tropicalis, trichoderma koningii, trichoderma reesei, rhodopseudomonas palustris, and Bacillus coagulans; the number of viable bacteria of bacillus amyloliquefaciens is 9-11 hundred million/ml, the number of viable bacteria of candida tropicalis is 8-10 hundred million/ml, the number of viable bacteria of trichoderma koningii is 6-8 hundred million/ml, the number of viable bacteria of trichoderma reesei is 5-7 hundred million/ml, the number of viable bacteria of rhodopseudomonas palustris is 2-6 hundred million/ml and the number of viable bacteria of bacillus coagulans is 2-4 hundred million/ml; preferably, the number of viable bacteria of Bacillus amyloliquefaciens is 10 hundred million/ml, the number of viable bacteria of Candida tropicalis is 9 hundred million/ml, the number of viable bacteria of Trichoderma koningii is 7 hundred million/ml, the number of viable bacteria of Trichoderma reesei is 6 hundred million/ml, the number of viable bacteria of Rhodopseudomonas palustris is 4 hundred million/ml, and the number of viable bacteria of Bacillus coagulans is 3 hundred million/ml.
In the invention, the dosage of the mixed vitamins in the feed is as follows: 9000-11000 IU/kg of vitamin A, 32400-3600 IU/kg of vitamin D, 34-6 mg/kg of vitamin K, 11-3 mg/kg of vitamin B, 27-9 mg/kg of vitamin B, 14-6 mg/kg of vitamin E, 35-45 mg/kg of nicotinic acid and 4-6 mg/kg of folic acid; preferably 10000IU/kg of vitamin A, 33000IU/kg of vitamin D, 35mg/kg of vitamin K, 12mg/kg of vitamin B, 28mg/kg of vitamin B, 15mg/kg of vitamin E, 40mg/kg of nicotinic acid and 5mg/kg of folic acid.
In the invention, the dosage of the mixed mineral in the feed is 900-1100 mg/kg of copper, 7500-8500 mg/kg of iron, 4000-6000 mg/kg of zinc, 4000-6000 mg/kg of manganese, 40-60 mg/kg of iodine, 30-40 mg/kg of chromium and 10-30 mg/kg of cobalt; preferably 1000mg/kg of copper, 8000mg/kg of iron, 5000mg/kg of zinc, 5000mg/kg of manganese, 50mg/kg of iodine, 35mg/kg of chromium and 20mg/kg of cobalt.
The invention also provides a preparation method of the beef cattle fermented feed, which comprises the following steps:
(1) Mixing the mixed grass meal with a fermentation microbial inoculum, adjusting the water content, and fermenting to obtain a material 1;
(2) Mixing and fermenting the material 1, the brewer's grains, the puffed soybeans, the bean curd residues and the mixed microbial inoculum to obtain a material 2;
(3) Mixing material 2, corn oil, molasses, salt, magnesium sulfate, sodium bicarbonate, mixed vitamins and mixed minerals, and drying.
In the invention, the water content is adjusted to 55-65% in the step (1); preferably 60%.
In the invention, the step (3) is dried until the water content is less than or equal to 14 percent; preferably 10 to 14%; more preferably 12%.
In the invention, the fermentation time of the step (1) is 7-11 d; preferably 9d.
In the invention, the fermentation time in the step (2) is 30-40 h; preferably 35h.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
A beef cattle fermented feed is prepared from the following components in parts by mass: 20 parts of brewer's grains, 20 parts of puffed soybeans, 20 parts of bean curd residues, 18 parts of mixed grass meal, 3 parts of corn oil, 3 parts of molasses, 3 parts of salt, 2 parts of magnesium sulfate, 2 parts of sodium bicarbonate, 4 parts of mixed vitamins, 4 parts of mixed minerals, 3 parts of mixed microbial agents and 3 parts of fermentation microbial agents.
The mixed grass meal comprises: pennisetum hydridum powder, peanut vine leaf powder, straw powder and alfalfa powder, wherein the mass ratio of the pennisetum hydridum powder to the peanut vine leaf powder to the alfalfa powder is 3:5:12:20.
the viable count of lactobacillus casei in the mixed microbial inoculum is 8 hundred million/ml, the viable count of acidophilic lactobacillus is 8 hundred million/ml, the viable count of pediococcus acidilactici is 6 hundred million/ml, the viable count of saccharomyces cerevisiae is 3 hundred million/ml, the viable count of acetobacter is 3 hundred million/ml, the viable count of aspergillus oryzae is 2 hundred million/ml, and the viable count of aspergillus niger is 2 hundred million/ml.
The number of viable bacteria of bacillus amyloliquefaciens in the fermentation microbial inoculum is 9 hundred million/ml, the number of viable bacteria of candida tropicalis is 8 hundred million/ml, the number of viable bacteria of trichoderma koningii is 6 hundred million/ml, the number of viable bacteria of trichoderma reesei is 5 hundred million/ml, the number of viable bacteria of rhodopseudomonas palustris is 2 hundred million/ml, and the number of viable bacteria of bacillus coagulans is 2 hundred million/ml.
The dosage of the mixed vitamins in the feed is as follows: 9000IU/kg of vitamin A, 32400IU/kg of vitamin D, 34mg/kg of vitamin K, 11mg/kg of vitamin B, 27mg/kg of vitamin B, 14mg/kg of vitamin E, 35mg/kg of nicotinic acid and 4mg/kg of folic acid.
The dosage of the mixed mineral in the feed is 900mg/kg of copper, 7500mg/kg of iron, 4000mg/kg of zinc, 4000mg/kg of manganese, 40mg/kg of iodine, 30mg/kg of chromium and 10mg/kg of cobalt.
The preparation method of the beef cattle fermented feed comprises the following steps:
(1) Mixing the mixed grass meal with a fermentation microbial inoculum, adjusting the water content to 55%, and fermenting for 7d to obtain a material 1;
(2) Mixing the material 1, the brewer's grains, the puffed soybeans, the bean curd residues and the mixed microbial inoculum, and fermenting for 30 hours to obtain a material 2;
(3) Mixing the material 2, corn oil, molasses, salt, magnesium sulfate, sodium bicarbonate, mixed vitamins and mixed minerals, and drying until the water content is 10%.
Example 2
A beef cattle fermented feed is prepared from the following components in parts by mass: 30 parts of brewer's grains, 30 parts of puffed soybeans, 28 parts of bean curd residues, 22 parts of mixed grass meal, 7 parts of corn oil, 7 parts of molasses, 5 parts of salt, 4 parts of magnesium sulfate, 4 parts of sodium bicarbonate, 8 parts of mixed vitamins, 8 parts of mixed minerals, 7 parts of mixed microbial agents and 7 parts of fermentation microbial agents.
The mixed grass meal comprises: pennisetum hydridum powder, peanut vine leaf powder, rice straw powder and alfalfa powder, wherein the mass ratio of pennisetum hydridum powder to peanut vine leaf powder to alfalfa powder is 8:9:16:30.
the viable count of lactobacillus casei in the mixed microbial inoculum is 12 hundred million/ml, the viable count of acidophilic lactobacillus is 12 hundred million/ml, the viable count of pediococcus acidilactici is 10 hundred million/ml, the viable count of saccharomyces cerevisiae is 5 hundred million/ml, the viable count of acetobacter aceti is 5 hundred million/ml, the viable count of aspergillus oryzae is 4 hundred million/ml, and the viable count of aspergillus niger is 4 hundred million/ml.
The number of viable bacteria of bacillus amyloliquefaciens is 11 hundred million/ml, the number of viable bacteria of candida tropicalis is 10 hundred million/ml, the number of viable bacteria of trichoderma koningii is 8 hundred million/ml, the number of viable bacteria of trichoderma reesei is 7 hundred million/ml, the number of viable bacteria of rhodopseudomonas palustris is 6 hundred million/ml and the number of viable bacteria of bacillus coagulans is 4 hundred million/ml.
The dosage of the mixed vitamins in the feed is as follows: 11000IU/kg of vitamin A, 33600IU/kg of vitamin D, 36mg/kg of vitamin K, 13mg/kg of vitamin B, 29mg/kg of vitamin B, 16mg/kg of vitamin E, 45mg/kg of nicotinic acid and 6mg/kg of folic acid.
The dosage of the mixed mineral in the feed is 1100mg/kg of copper, 8500mg/kg of iron, 6000mg/kg of zinc, 6000mg/kg of manganese, 60mg/kg of iodine, 40mg/kg of chromium and 30mg/kg of cobalt.
The preparation method of the beef cattle fermented feed comprises the following steps:
(1) Mixing the mixed grass meal with a fermentation microbial inoculum, adjusting the water content to 65%, and fermenting for 11d to obtain a material 1;
(2) Mixing the material 1, the brewer's grains, the puffed soybeans, the bean curd residues and the mixed microbial inoculum, and fermenting for 40 hours to obtain a material 2;
(3) Mixing the material 2, corn oil, molasses, salt, magnesium sulfate, sodium bicarbonate, mixed vitamins and mixed minerals, and drying until the water content is 14%.
Example 3
A beef cattle fermented feed is prepared from the following components in parts by mass: 25 parts of brewer's grain, 25 parts of puffed soybean, 24 parts of bean curd residue, 20 parts of mixed grass meal, 5 parts of corn oil, 5 parts of molasses, 4 parts of salt, 3 parts of magnesium sulfate, 3 parts of sodium bicarbonate, 6 parts of mixed vitamin, 6 parts of mixed mineral substance, 6 parts of mixed microbial inoculum and 6 parts of fermentation microbial inoculum.
The mixed grass meal comprises: pennisetum hydridum powder, peanut vine leaf powder, straw powder and alfalfa powder, wherein the mass ratio of the pennisetum hydridum powder to the peanut vine leaf powder to the alfalfa powder is 6:7:14:25.
the viable count of lactobacillus casei in the mixed microbial inoculum is 10 hundred million/ml, the viable count of acidophilic lactobacillus is 10 hundred million/ml, the viable count of pediococcus acidilactici is 8 hundred million/ml, the viable count of saccharomyces cerevisiae is 4 hundred million/ml, the viable count of acetobacter aceti is 4 hundred million/ml, the viable count of aspergillus oryzae is 3 hundred million/ml, and the viable count of aspergillus niger is 3 hundred million/ml.
The number of viable bacteria of bacillus amyloliquefaciens is 10 hundred million/ml, the number of viable bacteria of candida tropicalis is 9 hundred million/ml, the number of viable bacteria of trichoderma koningii is 7 hundred million/ml, the number of viable bacteria of trichoderma reesei is 6 hundred million/ml, the number of viable bacteria of rhodopseudomonas palustris is 4 hundred million/ml and the number of viable bacteria of bacillus coagulans is 3 hundred million/ml.
The dosage of the mixed vitamins in the feed is as follows: 10000IU/kg of vitamin A, 33000IU/kg of vitamin D, 35mg/kg of vitamin K, 12mg/kg of vitamin B, 28mg/kg of vitamin B, 15mg/kg of vitamin E, 40mg/kg of nicotinic acid and 5mg/kg of folic acid.
The dosage of the mixed mineral in the feed is 1000mg/kg of copper, 8000mg/kg of iron, 5000mg/kg of zinc, 5000mg/kg of manganese, 50mg/kg of iodine, 35mg/kg of chromium and 20mg/kg of cobalt.
The preparation method of the beef cattle fermented feed comprises the following steps:
(1) Mixing the mixed grass meal with a fermentation microbial inoculum, adjusting the water content to 60%, and fermenting for 9d to obtain a material 1;
(2) Mixing the material 1, the brewer's grains, the puffed soybeans, the bean curd residues and the mixed microbial inoculum, and fermenting for 35 hours to obtain a material 2;
(3) Mixing the material 2, corn oil, molasses, salt, magnesium sulfate, sodium bicarbonate, mixed vitamins and mixed minerals, and drying until the water content is 12%.
Experimental example 1
70 beef cattle (Simmental cattle) are selected, the weight range is 150 +/-5 kg, the raising time is 220d, 10 beef cattle are randomly distributed to each test group, and the beef cattle are freely fed.
The product obtained in example 3 was used as an experimental group;
control group 1: the difference from the example 3 is only that the mixed grass meal and the zymophyte agent are simply mixed and are not fermented;
control group 2: the difference from the embodiment 3 is only that the mixed grass meal is directly fermented, and the fermentation inoculum is replaced by equal amount of distilled water;
control group 3; the only difference from example 3 is that the materials in step (2) are simply mixed without fermentation;
control group 4; the difference from the embodiment 3 is only that the materials in the step (2) are directly fermented, and the mixed microbial inoculum is replaced by equal amount of distilled water;
control group 5: the fermentation inoculum and the mixed inoculum are replaced by equal amount of distilled water.
Control group 6: beef cattle feed of the prior art (CN 201310035844.5).
After completion of the experiment, the pre-slaughter body weight, meat yield and carcass weight of cattle were measured for the different test groups, and the results are shown in table 1.
TABLE 1
Weight kg before slaughtering The meat yield% Weight kg of cow carcass
Experimental group 514.9 51.4 236.2
Control group 1 504.6 48.7 226.7
Control group 2 482.1 47.1 210.3
Control group 3 498.5 48.3 225.0
Control group 4 491.2 46.9 213.4
Control group 5 451.3 45.2 187.7
Control group 6 489.8 47.8 206.8
As can be seen from the data in table 1, the data in the experimental group is optimal, the weight before slaughtering and the carcass weight of the cattle are the largest, and the meat yield is the largest and is significantly higher than those in other control groups, which indicates that the feed of the present invention has an excellent technical effect of promoting the growth of beef cattle.
Experimental example 2
Fermentation index parameters of rumen of beef cattle were measured on the last day of the experiment of experimental example 1, and the results are shown in table 2.
TABLE 2
Figure BDA0004042382030000091
Figure BDA0004042382030000101
As can be seen from Table 2, the pH of rumen fluid in the experimental group is controlled to be about 6.4, the buffering capacity is good, and the healthy state of rumen fermentation can be ensured. The low oxidation-reduction potential may be caused by a decrease in ORP due to a large amount of reducing substances contained in metabolites of rumen microorganisms after mass propagation. This demonstrates that the added beneficial bacteria of the present invention play a role.
Furthermore, the excessive ammonia nitrogen in the rumen can cause the ammonia nitrogen to be absorbed into the blood, so that the urea nitrogen in the blood is easy to be high, and therefore, the release amount of the ammonia nitrogen needs to be reduced, the ammonia nitrogen content of an experimental group is obviously reduced, and the fact that the feed of the experimental group can effectively reduce the release amount of the ammonia nitrogen in the rumen is proved; the most basic function of VFA in ruminant bodies is to provide energy, and as can be seen from table 2, different daily ration samples have different influences on the concentration of VFA in rumen, and compared with a control group, the total volatile fatty acid in an experimental group is remarkably improved, the fermentation function of the rumen of beef cattle can be effectively regulated and controlled, and the biological activity of volatile fatty acid and microorganisms is improved.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. The beef cattle fermented feed is characterized by being prepared from the following components in parts by mass: 20 to 30 portions of brewer's grain, 20 to 30 portions of puffed soybean, 20 to 28 portions of bean curd residue, 18 to 22 portions of mixed grass meal, 3 to 7 portions of corn oil, 3 to 7 portions of molasses, 3 to 5 portions of salt, 2 to 4 portions of magnesium sulfate, 2 to 4 portions of sodium bicarbonate, 4 to 8 portions of mixed vitamin, 4 to 8 portions of mixed mineral substance, 3 to 7 portions of mixed microbial inoculum and 3 to 7 portions of zymogen.
2. The fermented beef cattle feed as claimed in claim 1, wherein the mixed grass meal comprises: pennisetum hydridum powder, peanut vine leaf powder, straw powder and alfalfa powder, wherein the mass ratio of the pennisetum hydridum powder to the peanut vine leaf powder to the alfalfa powder is 3-8: 5 to 9:12 to 16:20 to 30.
3. The fermented feed for beef cattle according to claim 1, wherein the mixed microbial inoculum is lactobacillus casei, lactobacillus acidophilus, pediococcus acidilactici, saccharomyces cerevisiae, bacillus aceticus, aspergillus oryzae and aspergillus niger; the viable count of lactobacillus casei in the mixed microbial inoculum is 8-12 hundred million/ml, the viable count of acidophilic lactobacillus is 8-12 hundred million/ml, the viable count of pediococcus acidilactici is 6-10 hundred million/ml, the viable count of saccharomyces cerevisiae is 3-5 hundred million/ml, the viable count of acetobacter is 3-5 hundred million/ml, the viable count of aspergillus oryzae is 2-4 hundred million/ml, and the viable count of aspergillus niger is 2-4 hundred million/ml.
4. The fermented feed for beef cattle according to claim 1, wherein the fermentation inoculum is: bacillus amyloliquefaciens, candida tropicalis, trichoderma koningii, trichoderma reesei, rhodopseudomonas palustris, and Bacillus coagulans; the number of viable bacteria of bacillus amyloliquefaciens is 9-11 hundred million/ml, the number of viable bacteria of candida tropicalis is 8-10 hundred million/ml, the number of viable bacteria of trichoderma koningii is 6-8 hundred million/ml, the number of viable bacteria of trichoderma reesei is 5-7 hundred million/ml, the number of viable bacteria of rhodopseudomonas palustris is 2-6 hundred million/ml, and the number of viable bacteria of bacillus coagulans is 2-4 hundred million/ml.
5. The fermented feed for beef cattle as claimed in claim 1, wherein the mixed vitamins are used in the feed in the following amounts: 9000-11000 IU/kg of vitamin A, 32400-3600 IU/kg of vitamin D, 34-6 mg/kg of vitamin K, 11-3 mg/kg of vitamin B, 27-9 mg/kg of vitamin B, 14-6 mg/kg of vitamin E, 35-45 mg/kg of nicotinic acid and 4-6 mg/kg of folic acid.
6. The fermented feed for beef cattle according to claim 1, wherein the amount of the mixed minerals in the feed is 900-1100 mg/kg of copper, 7500-8500 mg/kg of iron, 4000-6000 mg/kg of zinc, 4000-6000 mg/kg of manganese, 40-60 mg/kg of iodine, 30-40 mg/kg of chromium and 10-30 mg/kg of cobalt.
7. The method for preparing a fermented feed for beef cattle according to any one of claims 1 to 6, comprising the steps of:
(1) Mixing the mixed grass meal with a fermentation microbial inoculum, adjusting the water content, and fermenting to obtain a material 1;
(2) Mixing and fermenting the material 1, the brewer's grains, the puffed soybeans, the bean curd residues and the mixed microbial inoculum to obtain a material 2;
(3) Mixing material 2, corn oil, molasses, salt, magnesium sulfate, sodium bicarbonate, mixed vitamins and mixed minerals, and drying.
8. The method for preparing fermented feed for beef cattle according to claim 7, wherein the water content in step (1) is adjusted to 55-65%.
9. The method for preparing fermented feed for beef cattle according to claim 7, wherein the step (3) is performed to dry the fermented feed to a water content of 14% or less.
10. The method for preparing the fermented feed for beef cattle according to claim 7, wherein the fermentation time in the step (1) is 7-11 days; the fermentation time in the step (2) is 30-40 h.
CN202310021039.0A 2023-01-07 2023-01-07 Beef cattle fermented feed and preparation method thereof Pending CN115868581A (en)

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