CN103255081A - Excellent bacillus coagulans for breeding sows and application thereof - Google Patents
Excellent bacillus coagulans for breeding sows and application thereof Download PDFInfo
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- CN103255081A CN103255081A CN2013100934577A CN201310093457A CN103255081A CN 103255081 A CN103255081 A CN 103255081A CN 2013100934577 A CN2013100934577 A CN 2013100934577A CN 201310093457 A CN201310093457 A CN 201310093457A CN 103255081 A CN103255081 A CN 103255081A
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Abstract
Excellent bacillus coagulans which is used in sow breeding and has effects of enhancing body immunologic functions, raising disease resistance and improving reproductive performance of pregnant sows is disclosed. The technical scheme is as follows: the bacillus coagulans HM-09 was preserved in the China General Microbiological Culture Collection Center on Oct. 29th, 2012; and the collection number is CGMCC No.6729. The invention also discloses an application method of the bacillus coagulans.
Description
Technical field
The present invention relates to a bacillus coagulans, but specifically be a strain enhancing body immunologic function, improve resistance against diseases and improve pregnant sow reproductive performance be used for Bacillus coagulans and the application thereof that sow is cultured.
Background technology
From the 1950's, microbiotic is introduced in the animal and fowl fodder, is bringing into play vital role as fodder additives aspect the growth that promotes animal, prevention pathogenic bacterial infection and the disease.Then, along with antibiotic long-term a large amount of the use, its all drawback as fodder additives is familiar with by people gradually, the life-time service microbiotic easily causes livestock and poultry autogenous infection and superinfection, cause flora imbalance in the livestock and poultry body, produce resistance, cause residues of antibiotics in the animal product, food safety and human health are had potential hazardness.
Along with improving constantly of constant development of economy and standard of living, people more and more recognize the basic meaning of " green ", if in feed, use probiotics, to reduce antibiotic consumption greatly, solve the problem that microbiotic can't resolve, enhance productivity, advance to the direction of green agriculture, whole livestock industry and feed are already had profound significance.
The present invention is directed to above-mentioned problem, develop the good Bacillus coagulans HM-09(Clostridium butyricum HM-09 that a broad sow is cultured), feed behind the sow as fodder additives, but enhancing body immunologic function, improve resistance against diseases, improve the reproductive performance of pregnant sow, improve the economic benefit that sow is cultured.
Summary of the invention
The good Bacillus coagulans and the application thereof that are used for the sow breed that the object of the present invention is to provide a strain enhancing body immunologic function, improve resistance against diseases and improve the reproductive performance of pregnant sow.
The present invention is achieved through the following technical solutions.
One strain is used for the good Bacillus coagulans that sow is cultured, it is characterized in that: described Bacillus coagulans HM-09(Bacillus coagulans HM-09) on October 29th, 2012, be Da Tun road, Chaoyang District, BeiJing, China city in the address, China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, preserving number: CGMCC No.6729.
Bacillus coagulans HM-09(Clostridium butyricum HM-09) separates in the health pig enteron aisle, Clostridium butyricum HM-09 can strengthen the sow body's immunity, improve resistance against diseases, the reproductive performance of balance intestinal microflora and raising pregnant sow.
A kind of activation of coagulating bacillus strain and cultivation, it is characterized in that: the Clostridium butyricum HM-09 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activation Clostridium butyricum HM-09 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g MnSO50mg adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
The using method of a kind of Bacillus coagulans in feed is characterized in that: the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet are stirred stand-by, the addition of Bacillus coagulans in feed is 1 * 10
5Cfu/g.
Clostridium butyricum HM-09 provided by the invention has following positively effect:
Experimental result shows, adds 1 * 10 in sow feed
5The Bacillus coagulans of cfu/g can effectively improve sow body's immunity and resistance against diseases, improves the economic benefit that sow is cultured.
Test-results shows, adds 1 * 10 in sow feed
5The Bacillus coagulans of cfu/g can effectively improve the reproductive performance of pregnant sow, improves litter size, produces live young number, birth weight and nascent litter weight.
Embodiment
The invention will be further described below in conjunction with embodiment; Following embodiment is illustrative, does not limit protection scope of the present invention.
One strain is used for the good Bacillus coagulans that sow is cultured, described Bacillus coagulans HM-09(Clostridium butyricum HM-09) on October 29th, 2012, be Da Tun road, Chaoyang District, BeiJing, China city in the address, China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, preserving number: CGMCC No.6729.
Bacillus coagulans HM-09(Clostridium butyricum HM-09) separates in the health pig enteron aisle, Clostridium butyricum HM-09 can strengthen the sow body's immunity, improve resistance against diseases, the reproductive performance of balance intestinal microflora and raising pregnant sow.
A kind of activation of coagulating bacillus strain and cultivation: the Clostridium butyricum HM-09 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activation Clostridium butyricum HM-09 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g MnSO50mg adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
The using method of a kind of Bacillus coagulans in feed: the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet are stirred stand-by, the addition of Bacillus coagulans in feed is 1 * 10
5Cfu/g.
Experimental technique among the following embodiment if no special instructions, is ordinary method.
The application of described Bacillus coagulans aspect the sow breed, concrete grammar is as follows:
Testing program: select 20 of ages, body weight, pregnant 55-60 days consistent with parity big Yorkshire sow of body condition, be divided into 2 groups at random, control group and test group, 10 every group, control group fed basal diet, the test group basal diet+0.1% Bacillus coagulans bacterium powder of feeding.Basal diet is formed and trophic level sees Table 1.
Table 1 basal diet is formed and trophic level
Index determining: add up the litter size of every sow, produce the young number of living, birth weight and nascent litter weight; Actual amount with the residue of clear bed material groove expects to add up feed consumes analysis integrated economic benefit according to feed and expenses for medicine at last.
The experimental animal management: two groups of feeding and management conditions are identical, routine operation by daily management carries out, sow of one circle, colony house is concrete floor, and all pig is at same pig house, by same personal management, day feeds 3 times, freely drink water, all sows carry out controlled feeding with the scale of feeding of average 2.5Kg/ day, control the fat condition that sow becomes at 7-8 all the time.
Experiment 1: Bacillus coagulans HM-09(Clostridium butyricum HM-09) to the sows farrowing Effect on Performance.
Select 20 of ages, body weight, pregnant 55-60 days consistent with parity big Yorkshire sow of body condition, be divided into control group and test group at random, every group 10, the control group fed basal diet, the feed bacterium powder of basal diet+0.1% Bacillus coagulans of test group, coagulated bacillus living number 〉=1 * 10 in the feed
5Cfu/g.Sow production breeding situation sees Table 2.
Table 2 sow production breeding situation
As seen from the experiment, the test group nest on average produces the young number of living and has improved 7% than control group, and average individual birth weight has improved 9.8% than control group, and on average nascent nest consequence has improved 6.2%, the average weak young number of the average dead young number of nest and nest has reduced by 71% and 91% respectively, significant difference (P＜0.05).
Experiment 2: Bacillus coagulans HM-09(Clostridium butyricum HM-09) to the improvement of sow feed utilization ratio.
Select 20 of ages, body weight, pregnant 55-60 days consistent with parity big Yorkshire sow of body condition, be divided into control group and test group at random, every group 10, the control group fed basal diet, the feed bacterium powder of basal diet+0.1% Bacillus coagulans of test group, coagulated bacillus living number 〉=1 * 10 in the feed
5Cfu/g, test period 60 days.The duration of test sow sees Table 3 to the feed intake situation.
Table 3 duration of test sow is to the feed intake situation
By table 3 test-results as can be known, during the whole test, the equal feed consumption 148.2kg of test group head, the equal feed consumption 155kg of control group head, test group reduces by 4.4% than the equal consumption of control group head, and cost reduces by 2.1%.
Experiment 3: Bacillus coagulans HM-09(Clostridium butyricum HM-09) improve the sow medicining condition.
Select 20 of ages, body weight, pregnant 55-60 days consistent with parity big Yorkshire sow of body condition, be divided into control group and test group at random, every group 10, the control group fed basal diet, the feed bacterium powder of basal diet+0.1% Bacillus coagulans of test group, coagulated bacillus living number 〉=1 * 10 in the feed
5Cfu/g, test period 60 days.Duration of test sow medicining condition sees Table 4.
Table 4 duration of test sow medicining condition
By table 4 test-results as can be known, the control group drug cost is 58.45 yuan, and the test group drug cost is 16.55 yuan, and test group reduces cost 41.9 yuan than control group, illustrates that the sow Bacillus coagulans of feeding can improve the resistance against diseases of sow.
Experiment 4: sow Bacillus coagulans HM-09(Clostridium butyricum HM-09 feeds) to the lifting situation of sow economic benefit.
Statistical study is carried out in the equal feed consumption rate of duration of test sow head, the equal medication cost of head, be the results are shown in Table 5.
Table 5 Economic and Efficiency Analysis before and after the Bacillus coagulans of feeding
By table 5 test-results as can be known, the sow coagulated bacillus living formulation of feeding, the sow sickness rate reduces, and feed consumption rate reduces, and the feeding cost of average every the sow of duration of test (60 days) has reduced by 8.19 yuan, has improved the economic benefit that sow is cultured.
Claims (4)
1. a strain is used for the good Bacillus coagulans that sow is cultured, and it is characterized in that: be to separate to obtain from the health pig enteron aisle described Bacillus coagulans HM-09(Bacillus coagulans HM-09); This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number: CGMCCNo.6729, preservation date are on October 29th, 2012.
2. the application of a Bacillus coagulans is characterized in that: the application of described Bacillus coagulans aspect the sow breed.
3. the activation of a Bacillus coagulans and cultivation, it is characterized in that: the Bacilluscoagulans HM-09 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activated b acillus coagulans HM-09 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g, MnSO
450mg adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
4. the using method of a Bacillus coagulans in feed is characterized in that: the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet are stirred stand-by, Bacillus coagulansHM-09 viable count reaches 1 * 10 in the feed
5Cfu/g.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243043A (en) * | 2013-03-22 | 2013-08-14 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for improving inferior piglet breeding and application of bacillus coagulans |
| CN103255082A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding finishing pigs and application thereof |
| CN104017751A (en) * | 2014-04-17 | 2014-09-03 | 青岛九和宜生生物科技有限公司 | Probiotics prepared from bacillus coagulans and lactobacillus plantarum as well as preparation method thereof |
| CN106578531A (en) * | 2016-12-29 | 2017-04-26 | 四川省旺达饲料有限公司 | Compound feed special for perinatal period sows |
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| CN102505003A (en) * | 2011-12-19 | 2012-06-20 | 湖南省微生物研究所 | High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof |
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2013
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Patent Citations (1)
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| CN102505003A (en) * | 2011-12-19 | 2012-06-20 | 湖南省微生物研究所 | High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof |
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| 周映华 等: "不同芽孢杆菌对断奶仔猪生产性能的影响", 《饲料工业》 * |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243043A (en) * | 2013-03-22 | 2013-08-14 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for improving inferior piglet breeding and application of bacillus coagulans |
| CN103255082A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding finishing pigs and application thereof |
| CN103243043B (en) * | 2013-03-22 | 2015-09-16 | 内蒙古和美科盛生物技术有限公司 | One strain is for improving excellent Bacillus coagulans and the application thereof of the cultivation of inferior piglet |
| CN104017751A (en) * | 2014-04-17 | 2014-09-03 | 青岛九和宜生生物科技有限公司 | Probiotics prepared from bacillus coagulans and lactobacillus plantarum as well as preparation method thereof |
| CN104017751B (en) * | 2014-04-17 | 2017-03-29 | 青岛九和宜生生物科技有限公司 | Bacillus coagulans, Lactobacillus plantarum and Lactobacillus casei preparation and its preparation |
| CN106578531A (en) * | 2016-12-29 | 2017-04-26 | 四川省旺达饲料有限公司 | Compound feed special for perinatal period sows |
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| CN103255081B (en) | 2015-05-27 |
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