CN103884700B - Fluorescent whitening agent detector and detection method - Google Patents
Fluorescent whitening agent detector and detection method Download PDFInfo
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- CN103884700B CN103884700B CN201410114384.XA CN201410114384A CN103884700B CN 103884700 B CN103884700 B CN 103884700B CN 201410114384 A CN201410114384 A CN 201410114384A CN 103884700 B CN103884700 B CN 103884700B
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Abstract
The present invention discloses a kind of fluorescent whitening agent detector, comprises body, and body upper surface is provided with display screen and fluoroscopic examination module, and fluoroscopic examination module comprises detection main body and detection cell. The invention also discloses the detection method of this kind of fluorescent whitening agent detector, after testing sample is put into detection cell, put into detection main body, detection, reading. The detector of the present invention can quick and precisely, the on-the-spot white dyes to product in ground easy to carry carry out detection by quantitative whenever and wherever possible; The detection method of this detector is easy and simple to handle, detection is accurately quick.
Description
Technical field
The present invention relates to a kind of detecting instrument and detection method thereof, particularly relate to a kind of fluorescent whitening agent detector and detection method.
Background technology
White dyes is commonly called as white dyestuff, is a kind of fluorescence dye, belongs to the organic compound of a kind of complexity. Its characteristic can excite to absorb incident ultraviolet light generation fluorescence, namely the visible ray producing wavelength in 400nm-600nm scope is excited after absorbing UV-light (wavelength region is about 360nm-380nm), thus can compensate undesired micro-yellow in matrix, thus item color visually seem more in vain, brighter, more bright-coloured. Due to this kind of characteristic, water-soluble white dyes is widely used in food, paper, coating, washing powder and cotton fabric etc., and water-insoluble white dyes is then applied to the product such as chemical fibre, plastics.
Having research display in the recent period, white dyes is a kind of potential carcinogens. When the products such as medicated napkin containing white dyes, cotton fabric and human contact, white dyes meeting and skin contact under the soaking into of water or sweat, be absorbed by the body; Or contact with oral cavity, eat in body, HUMAN HEALTH is existed very important harm. The detection by quantitative of white dyes is mainly carried out by current China in the environment in laboratory, and detecting instrument is bulky, and inconvenience is carried. The instrument of existing Site Detection white dyes is the detection for living paper products, structure, see Fig. 4 and Fig. 5, comprises body 1 ', the display screen 2 ' on body 1 ' surface and the fluorescence excitation module 3 ' of setting in key panel 4 ', body 1 ' and excited carriers module 5 '. Wherein, testing sample loads in excited carriers module 5 ', fluorescence excitation module 3 ' is together inserted with it, fluorescence excitation module 3 ' has two light sources 32 ' and 34 ' level directly to penetrate to testing sample and excite generation fluorescence, the fluorescence produced is through detection passage 33 ', being displayed on display screen 2 ' by the fluorescence intensity after process with the form of fluorescent brightener levels, detection passage 33 ' comprises spectral filter and photoelectric detector. But the detected result error of this kind of detector is big, and accuracy is low, it is very difficult to widespread use. Therefore, be badly in need of at present a kind of instrument can quick and precisely, ground easy to carry is on-the-spot that white dyes is carried out detection by quantitative whenever and wherever possible.
Summary of the invention
The main purpose of the present invention is for the technological deficiency existed in prior art, and provides the detector of a kind of easy to carry, Quantitative detection white dyes.
It is a further object to provide the detection method of above-mentioned fluorescent whitening agent detector.
The technical scheme adopted for realizing the object of the present invention is:
Fluorescent whitening agent detector, comprise body, body upper surface is provided with display screen and fluoroscopic examination module, fluoroscopic examination module comprises detection main body and detection cell, detection main body comprises hollow thicl-walled cylinder shape and is axially uniformly distributed the framework body of some through holes in barrel along cylinder, the via bottoms of framework body is provided with light source, and framework intracoelomic cavity is provided with optical signal detector, spectral filter, Fresnel Lenses, biconvex lens and detection cell from the bottom to top successively.
The upper outside of framework body is provided with the agent localization ring for being fixed on body by detection main body.
The inwall on framework body top forms the lens step for placing biconvex lens lower than outer wall.
Biconvex lens is fixed on the lens step of framework body by lens position ring fixing on biconvex lens.
The Fresnel Lenses being fixed together becomes combined lens to be fixed on above optical signal detector by lens orientation ring with filter set.
Described detection cell comprises detection cell main body and gland, detection cell lower body part is the platform for placing testing sample that a diameter is less than detection cell main diameter, gland for nested described platform is made up of the vertical edge being provided with the bottom surface of a detection window and surrounding is surrounded, and with platform shrink-fit.
The height of described platform is greater than the height on the vertical edge of described gland.
A kind of white dyes detection method, the light that light source is penetrated forms parallel light and injects a biconvex lens, and the focus position that testing sample is placed in biconvex lens is excited generation fluorescence; Make fluorescence instead become collimated beam after biconvex lens, first focus on through Fresnel Lenses and strengthen fluorescent signal, then converge to optical signal detector after spectral filter elimination stray light, determine the content of white dyes in testing sample finally by fluorescence intensity value.
Above-mentioned detection method employs above-mentioned fluorescent whitening agent detector, after sheet testing sample is filled the detection window of full detection cell, detection cell is placed on above the detection main body being fixed in body, power-on, start detection, read the fluorescence content value on display screen.
Above-mentioned detection method is that detection cell main body is turn to platform upward, and testing sample is placed on platform, compress gland, detection window is all filled by testing sample, then turn to platform down after, detection cell is placed on detection main body above.
Compared with prior art, the invention has the beneficial effects as follows: above-mentioned fluorescent whitening agent detector be a kind of can quick and precisely, the on-the-spot white dyes to product in ground easy to carry carry out the instrument of detection by quantitative whenever and wherever possible; Its middle frame body inside is containing multiple cylindrical hole, the light that light source penetrates can be formed parallel light and inject biconvex lens, testing sample is in the focus position of biconvex lens, light is the strongest and distribution of light is even, and fluorescence excitation is effective, compared to existing detector only two light sources, the light being mapped on testing sample easily distributes inequality, it is also possible to light intensity is inadequate, what all can affect fluorescence excites degree, thus causes detected result inaccurate; Fresnel Lenses is also had to strengthen optical signal, again through spectral filter elimination stray light, obtain pure fluorescent signal, reduce metrical error, make result more accurate, and it is little to take space, be conducive to instrument miniaturization, and existing detector testing process excites the fluorescence obtained only after filtration mating plate process just carry out the detection of fluorescence content, when fluorescence content value is lower, such detected result error is bigger; In addition, optical signal detector, on the center of circle being evenly equipped with multiple arbitrary source circumference, makes whole light source, light channel structure simple. The detection method of this detector is easy and simple to handle, detection is accurately quick, in Site Detection sample, the content of white dyes provides reliable detection means.
Accompanying drawing explanation
Fig. 1 show the external structure of the fluorescent whitening agent detector of the present invention;
Fig. 2 is the structure iron of the fluoroscopic examination module of detector of the present invention;
Fig. 2 a is the framework body vertical view of detector of the present invention;
The light source that Fig. 2 b is detector of the present invention launches the three-dimensional structure diagram receiving integrated apparatus with optical signal;
Fig. 2 c is the detection cell structure iron of detector of the present invention;
Fig. 2 d is the three-dimensional structure diagram of the detection cell main body of Fig. 2 c detection cell;
Fig. 2 e is the three-dimensional structure diagram of the gland of Fig. 2 c detection cell;
Fig. 3 show in the detection method of the present invention utilize index path;
Fig. 4 show the external structure of existing fluorescent whitening agent detector;
Fig. 5 show the structure iron of fluorescence excitation module in existing fluorescent whitening agent detector.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail. It is to be understood that specific embodiment described herein is only in order to explain the present invention, it is not intended to limit the present invention.
Fluorescent whitening agent detector provided by the invention, its outer bilge construction is see Fig. 1, comprise body 1, body 1 upper surface is provided with liquid crystal display 2 and fluoroscopic examination module 3, the side of body 1 is provided with power switch 5, supply socket 4 and key panel, wherein key panel comprises upper and lower key, return, confirm key etc., operation respective keys allows instrument complete multinomial contents of program after powering, such as, upper and lower key can make program enter or the next item down, returns and program can be made to return to zero position, presses and confirms that key program can formally start. Internal body is equipped with microprocessor, chip, storer and circuit card etc.
The structure of fluoroscopic examination module 3 as shown in Figure 2, comprises detection main body 30 and detection cell 37.
In Fig. 2, detection main body 30 comprises a framework body 31, this framework body 31 is the bottomless cylindrical tube shape of the thick wall of hollow, and axially it is uniformly distributed eight cylindrical holes 312 along cylinder in barrel, the center of circle of these eight cylindrical holes 312 is circumferentially same, and the bottom surface of this circumference and framework body 31 is that concentric (its vertical view is see Fig. 2 a). the outside on outer wall 315 top of this framework body 31 establishes one with the agent localization ring 39 of internal thread, and this agent localization ring 39 is together by a threaded connection with framework body, thus detection main body 30 is fixed on body 1. the outer wall 315 of framework body 31 is higher than inwall 314, forms lens step 310 for placing biconvex lens 36, and lens step 310 is arranged on about 2/3 highly place of framework body 31 outer wall 315. placing a biconvex lens 36 on lens step 310, in order to make biconvex lens more firm, establish a lens position ring 38 at its edge, biconvex lens 36 is fixed on lens step 310 by this lens position ring 38 by screw thread. the middle bottom of framework body 31 is provided with one group of combined lens, this group eyeglass is combined into one by spectral filter 34 and Fresnel Lenses 35, wherein Fresnel Lenses is upper, spectral filter under, two eyeglasses are fixed in framework body 31 by lens orientation ring 33, outside screw on lens orientation ring 33 is connected with the screw-internal thread fit on framework body 31 inwall 314, Fresnel Lenses in combined lens can converge low light signals better, spectral filter can elimination stray light, make fluorescent signal purer, thus improve the accuracy of detected result, reduce metrical error, and its to take space little, be conducive to instrument miniaturization.
Detection main body 30 is also included in the light source bottom framework body 31 and launches with optical signal reception its three-dimensional structure diagram of integrated device 32(see Fig. 2 b), this device 32 comprises a disk 321, this disk 321 is identical with the shape of framework body 31 bottom surface, it is provided with optical signal detector 322 in the central position of disk 321 upper surface, disk 321 is fixed on the bottom surface of bottom as framework body 31 of framework body 31, and optical signal detector 322 stretches in the cylindrical drum of framework body 31, it is positioned at the lower section of lens orientation ring 33. Eight LED/light source 323 it are uniformly distributed on disk 321, around optical signal detector 322, these light sources 323 are corresponding with cylindrical hole 312 and stretch into wherein, so, cylindrical hole can play the effect of collimator tube, and the light of light source injection is by forming parallel uniform light after cylindrical hole.
In Fig. 2, detection cell 37 is located at above biconvex lens 36, is also exactly the top of detection main body 30. Detection cell 37 comprises detection cell main body 372 and gland 371(such as Fig. 2 c). Detection cell main body 372 top is a boss 373, conveniently takes, detection cell main body 372 bottom inwardly shortens a platform 376 into, and the diameter of this platform 376 is slightly less than detection cell main body 372, and the bottom surface of platform 376 is used for placing testing sample. Gland 371 bottom center has a detection window 377, and this detection window is a through hole, is provided with a circle vertical along 375 around gland 371, referring to Fig. 2 e, gland bottom surface and the vertical platform 376 being used for nested detection cell lower body part along 375 spaces encircled a city, and with its shrink-fit. Owing to the diameter of platform is less than detection cell main body, therefore, forming a platform step 374 between platform 376 and detection cell main body 372, in order to make gland and platform coordinate better, it is identical that the height of platform step founds the height along 375 with gland; During use, detection cell main body being put upside down, make platform upward, sheet testing sample is placed on platform, covers and compresses gland, and testing sample is revealed from detection window, then by the platform of detection cell main body down, insertion detection main body; Like this, not only the shape of testing sample is unrestricted, and the area of detection of all samples is all unified, and detected result is more reliable.
The circuit connecting relation of the present invention is, microprocessor circuit conventional with light source 323, optical signal detector 322 and liquid crystal display 2 is connected, the open and close of Microprocessor S3C44B0X light source, light source inspires the fluorescence of testing sample in detection cell 37 by detection main body 30, the fluorescent signal collected is fed back to microprocessor by optical signal detector 322, optical signal prosessing is just become numerary signal by microprocessor, then fluorescence content value is displayed on liquid crystal display.
In the present embodiment, all parts all adopt conventional commercially available prod, and such as, optical signal detector adopts the integrated optic-electronic sensor of Bin Song company of Japan production, and model is S2684 series; Microprocessor is the 8-bit microcontroller of the STC89 series that SST company produces, and storage inside device is 64K. Other have all alternative the said products of product of equivalent efficacy.
The assembling process of above-mentioned fluoroscopic examination module is as follows: 1) is launched by light source and is fixed on bottom framework body 31 with optical signal reception integrated device 32; 2) above optical signal detector 322, Fresnel Lenses and spectral filter is installed by lens orientation ring 33; 3) by lens position ring 38, biconvex lens 36 is fixed on lens step 310 again; 4) the above-mentioned detection main body 30 installed is fixed on body by agent localization ring 39; 5) detection cell that testing sample is housed finally is put into.
Detection method and the principle of work of above-mentioned fluorescent whitening agent detector provided by the invention are, first detection cell main body 372 is put upside down, platform 376 is upward, sheet testing sample is placed on platform 376, gland 371 is buckled on platform 376 and compresses, make to appear in the detection window 377 on gland 371 whole be testing sample, upset detection cell 37, make gland 371 behind lower section, put it in the detection main body 30 being fixed on body 1, power-on 5; The all or part of LED/light source 323 of Microprocessor S3C44B0X launches light, and index path is shown in Fig. 3, and light forms collimated beam through cylindrical hole 312 and injects biconvex lens 36, is then mapped on the testing sample of detection cell 37 through its focusing, excites generation fluorescence; Fluorescence becomes again collimated beam after biconvex lens 36, first focuses on through Fresnel Lenses 35 and strengthens fluorescent signal, then converges on optical signal detector 322 after spectral filter 34 elimination stray light; The microprocessor processes of optical signal through being attached thereto becomes detection numerical value, microprocessor prestores the database that the fluorescence content value of a large amount of actual sample is aggregated into corresponding fluorescence intensity data, comparing in the fluorescence intensity level recorded and database, associate with corresponding fluorescence content value again, finally fluorescence content value is reflected on liquid crystal display 2, reads.
Testing sample in aforesaid method can be the plane sample that the scraps of paper, pieces of cloth etc. can be pressed in detection cell; Time detection liquid (such as washing liquid etc.) or nonplanar solid (sample such as steamed bun such as pulverous washing powder and irregular shape), can infiltrate on the scraps of paper or pieces of cloth after conventionally the white dyes in sample being dissolved, then carry out detecting.
The fluorescent whitening agent detector and Turner company of the U.S. fluorophotometer Quantech(model that use the present invention are FM109515-33) carry out contrast experiment, testing sample selects certain brand washing composition, certain brand medicated napkin and certain brand clothing, detected result respectively in Table 1-3, to verify the reliability of the experimental result that fluorescent whitening agent detector of the present invention and detection method thereof obtain.
The fluoroscopic examination result of table 1 washing composition
The fluoroscopic examination result of table 2 medicated napkin
The fluoroscopic examination result of table 3 clothing
Contrast three groups of data, it has been found that this detector does not exist deviation with the detected result mean value of fluorophotometer, therefore demonstrates the reliability of fluorescent whitening agent detector provided by the invention and detection method thereof.
The above is only the preferred embodiment of the present invention; it should be appreciated that for those skilled in the art, under the premise without departing from the principles of the invention; can also making some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. fluorescent whitening agent detector, comprise body, body upper surface is provided with display screen and fluoroscopic examination module, it is characterized in that, fluoroscopic examination module comprises detection main body and detection cell, detection main body comprises hollow thicl-walled cylinder shape and is axially uniformly distributed the framework body of some through holes in barrel along cylinder, and the via bottoms of framework body is provided with light source, and framework intracoelomic cavity is provided with optical signal detector, spectral filter, Fresnel Lenses, biconvex lens and detection cell from the bottom to top successively.
2. fluorescent whitening agent detector according to claim 1, it is characterised in that, the upper outside of framework body is provided with the agent localization ring for being fixed on body by detection main body.
3. fluorescent whitening agent detector according to claim 2, it is characterised in that, the inwall on framework body top forms the lens step for placing biconvex lens lower than outer wall.
4. fluorescent whitening agent detector according to claim 3, it is characterised in that, biconvex lens is fixed on the lens step of framework body by lens position ring fixing on biconvex lens.
5. fluorescent whitening agent detector according to claim 4, it is characterised in that, the Fresnel Lenses being fixed together becomes combined lens to be fixed on above optical signal detector by lens orientation ring with filter set.
6. according to the claim 1-5 described fluorescent whitening agent detector of arbitrary item, it is characterized in that, described detection cell comprises detection cell main body and gland, detection cell lower body part is the platform for placing testing sample that a diameter is less than detection cell main diameter, gland for nested described platform is made up of the vertical edge being provided with the bottom surface of a detection window and surrounding is surrounded, and with platform shrink-fit.
7. fluorescent whitening agent detector according to claim 6, it is characterised in that, the height of described platform is greater than the height on the vertical edge of described gland.
8. a white dyes detection method, it is characterized in that, use fluorescent whitening agent detector described in claim 6 or 7, after sheet testing sample is filled the detection window of full detection cell, detection cell is placed on above the detection main body being fixed in body, power-on, starts detection, the light that light source is penetrated forms parallel light and injects a biconvex lens, and the focus position that testing sample is placed in biconvex lens is excited generation fluorescence; Fluorescence is made instead to become collimated beam after biconvex lens, first focus on through Fresnel Lenses and strengthen fluorescent signal, optical signal detector is converged to again after spectral filter elimination stray light, determine the content of white dyes in testing sample finally by fluorescence intensity value, read the fluorescence content value on display screen.
9. white dyes detection method according to claim 8, it is characterized in that, it is that detection cell main body is turn to platform upward, testing sample is placed on platform, compress gland, detection window is all filled by testing sample, then turn to platform down after, detection cell is placed on detection main body above.
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| CN104897629A (en) * | 2015-05-15 | 2015-09-09 | 吉林大学 | Method for in-situ detection of migration quantity of fluorescent whitening agent |
| CN105259148B (en) * | 2015-11-02 | 2019-05-14 | 深圳市锦瑞生物科技有限公司 | A kind of fluorescence immunoassay quantitative analysis instrument |
| CN106770097B (en) * | 2016-12-01 | 2019-06-18 | 广州市盛邦化工科技有限公司 | The detection device of fluorescer in a kind of cleaning product |
| CN109682812B (en) * | 2016-12-01 | 2021-05-04 | 江苏信众知识产权运营管理有限公司 | Fluorescent agent image distinguishing method and fluorescent agent detection method |
| CN106770108B (en) * | 2016-12-13 | 2023-10-27 | 长春海关技术中心 | Method for detecting toxic substances based on nano optical fiber biosensor |
| CN109521012B (en) * | 2018-12-29 | 2021-06-01 | 深圳市朴瑞生物科技有限公司 | Inverted instant testing device for POCT detection |
| CN110530828A (en) * | 2019-07-23 | 2019-12-03 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of Portable fluorescence detection device and its control method |
| CN110806382B (en) * | 2019-11-22 | 2022-06-28 | 长春美泰仪器有限公司 | Integrated liquid and solid detection pool device and fluorescence detector |
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| US4018530A (en) * | 1975-11-18 | 1977-04-19 | Block Engineering, Inc. | Fluorescence spectrometry employing excitation of bleaching intensity |
| CN200968935Y (en) * | 2006-11-21 | 2007-10-31 | 杭州远方光电信息有限公司 | Exciting and receiving apparatus for luminous diode fluorescent powder test |
| CN201397298Y (en) * | 2009-03-20 | 2010-02-03 | 郝书顺 | Dual-channel reflection-type fluorescence sensor |
| CN201837587U (en) * | 2010-04-23 | 2011-05-18 | 上海互帼科学仪器有限公司 | Portable fluorescence detecting instrument |
| CN102495036A (en) * | 2011-12-07 | 2012-06-13 | 长春美泰科技有限公司 | On-site detector for fluorescent bleaching agent in living paper products |
| CN102590161A (en) * | 2012-01-16 | 2012-07-18 | 长春美泰科技有限公司 | Fluorescent whitening agent field detecting instrument |
| CN203259476U (en) * | 2013-05-11 | 2013-10-30 | 徐培实 | Portable flourescent brightener detector |
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