CN110530828A - A kind of Portable fluorescence detection device and its control method - Google Patents
A kind of Portable fluorescence detection device and its control method Download PDFInfo
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- CN110530828A CN110530828A CN201910666138.8A CN201910666138A CN110530828A CN 110530828 A CN110530828 A CN 110530828A CN 201910666138 A CN201910666138 A CN 201910666138A CN 110530828 A CN110530828 A CN 110530828A
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- inner cylinder
- shell
- detection device
- fluorescence detection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- Life Sciences & Earth Sciences (AREA)
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- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A kind of Portable fluorescence detection device and its control method, including control system and shell, inner cylinder is equipped in the shell, inner cylinder is the hollow structure of both ends open, circulation canal is equipped between inner cylinder and shell, outer casing bottom activity is equipped with stirring blade, the stirring blade protrudes into inner cylinder, enclosure bottom attaching has mounting base, motor is equipped in mounting base, the drive shaft of motor is equipped with swivel plate, it is connected between stirring blade and swivel plate by magnetic force mechanism, enclosure bottom is equipped with the heater connecting with control system, side wall of outer shell is equipped with the temperature sensor connecting with control system, ultraviolet emissioning tube and fluorescence reception sensor, motor is connect with control system, ultraviolet emissioning tube is received by fluorescence reception sensor after reflection towards emitting ultraviolet light line in circulation canal.Operation of the present invention is convenient, and small volume is convenient for carrying, and measurement accuracy is high.
Description
Technical field
The present invention relates to a kind of detection device, specifically a kind of Portable fluorescence detection device and its control method.
Background technique
Fluorescer also known as fluorescent whitening agent are a kind of fluorescent dyes, or for white dye and a kind of complicated are had
Machine compound.Its characteristic is to absorb incident ray to generate fluorescence, and contaminated substance is made to obtain the sparkling of similar fluorite
Effect, the substance for seeing naked eyes is very white, achievees the effect that brighten.
The effect of fluorescer is that the sightless ultraviolet radiation that product is absorbed is transformed into indigo fluorescent radiation, with
Complementary color becomes white light each other for original yellow light radiation, improves the whiteness of product in the sunlight.Brightening agent has been widely used in spinning
It knits, papermaking, washing powder, liquid detergent, soap, rubber, plastics, pigment and paint etc..
Since fluorescent whitening agent is after absorbing black light (wave-length coverage is between 300nm~400nm), in molecule
Then electrons are returned to ground state from ground state transition in a very short period of time, while launching blue or purple fluorescence (wave-length coverage
Between 420nm~480nm).Therefore, under the ultraviolet light irradiation of wavelength 365nm, by observing whether sample has obvious fluorescence existing
As coming in qualitative determination sample whether contain fluorescent whitening agent.If there is the discontinuous stigma punctate fluorescence in many places or sample in sample
Have fluorescence phenomenon but it is unobvious when, can be extracted with alkaline extracting solution, extracting solution is then adjusted to acidity, then mentioned with gauze absorption
The fluorescent whitening agent in liquid is taken, under wavelength 365nm ultraviolet lamp, whether observation gauze has obvious fluorescence phenomenon, to confirm sample
In whether contain fluorescent whitening agent.
The content of traditional detection determinand fluorescer, process is complicated, needs to do more pre-treatment work, and environmental requirement is severe
It carves, is not easy to portable quick detection.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Portable fluorescence detection device and its control methods.
In order to solve the above-mentioned technical problem, the present invention takes following technical scheme:
A kind of Portable fluorescence detection device, including control system and shell, the shell is interior to be equipped with inner cylinder, inner cylinder
For the hollow structure of both ends open, circulation canal is equipped between inner cylinder and shell, outer casing bottom activity is equipped with stirring blade, should
Stirring blade protrudes into inner cylinder, and enclosure bottom attaching has mounting base, is equipped with motor in mounting base, the drive shaft of motor is equipped with
Swivel plate, between stirring blade and swivel plate by magnetic force mechanism connect, enclosure bottom be equipped with connect with control system add
Hot device, side wall of outer shell are equipped with temperature sensor, ultraviolet emissioning tube and the fluorescence reception sensor connecting with control system, motor
It is connect with control system, ultraviolet emissioning tube is towards emitting ultraviolet light line in circulation canal after reflection by fluorescence reception sensor
It receives.
The circulation canal includes that the interlayer being located between upper through-hole, inner cylinder and the outer housing of inner cylinder side wall upper part leads to
Lower gap between road and inner cylinder lower end and outer housing inner bottom surface, through-hole, sandwich passage and lower gap form feed flow body on this
The circulation canal of flowing.
The inner cylinder upper end is equipped with seal cover board, which covers upper shell simultaneously.
The stirring blade includes at least two blades, and a blade magnet, and phase are installed in the bottom surface of each blade
The polarity of adjacent blade magnet is opposite;Swivel plate is equipped with adsorption magnet, the quantity and the quantity phase of blade magnet of adsorption magnet
With and polarity correspond to it is identical.
The swivel plate has leaflet plate, and the quantity of leaflet plate is equal with the quantity of the blade on stirring blade.
The shell inner bottom surface is equipped with rotor fixed position block.
The bottom surface of the seal cover board is equipped with step position, and the upper end of shell, which is supported, is mounted on the step position.
The control system includes MCU main control module, and the ultraviolet light emission mould connecting respectively with the MCU main control module
Block, fluorescence detection module, heating module, temperature detecting module, rotation control module, man machine operation interface, communication interface sum number
According to storage center.
A kind of control method of Portable fluorescence detection device, comprising the following steps:
Sample to be tested is added in inner cylinder and dissolution liquid, set temperature section, heater are begun to warm up, makes shell
Temperature is maintained in set temperature section;
Motor drives stirring blade rotation, stirs to form testing liquid to sample to be tested and dissolution liquid crushing, prepare liquid
Body flows in circulation canal to be uniformly distributed;
Ultraviolet emission pipe emitting ultraviolet light line, reflects again after testing liquid is irradiated by ultraviolet light and is sensed by fluorescence reception
Device, which receives, reads fluorescent value;
The fluorescent value read is sent to control system, analysis is compared with the fluorescent value of setting, obtains as a result, simultaneously
It is stored, is detected by multiple repairing weld, averaged, the fluorescence of as current sample to be tested.
It is described by temperature sensor real-time detection skin temperature, pass through control system and control the unlatching of heater, close
Or the adjustment of power.
The present invention is rotated using magnetic drive stirring blade, so that generally closed system, easy to clean, reduces raffinate
Pollution, easy to operate, detection accuracy is high.
Detailed description of the invention
Attached drawing 1 is schematic diagram of the three-dimensional structure;
Attached drawing 2 is that the present invention removes the cut-away diagram after shell and inner cylinder;
Attached drawing 3 is partial cutaway schematic of the present invention;
Attached drawing 4 is the elevational schematic view of stirring blade in the present invention;
Attached drawing 5 is the schematic diagram of control system of the present invention;
Attached drawing 6 is that ultraviolet light emission of the present invention conducts schematic diagram.
Specific embodiment
To further understand the features of the present invention, technological means and specific purposes achieved, function, below with reference to
Present invention is further described in detail with specific embodiment for attached drawing.
As shown in figures 1-6, present invention discloses a kind of Portable fluorescence detection device, including control system and shell 1,
Inner cylinder 2 is equipped in the shell 1, inner cylinder 2 is the hollow structure of both ends open, and circulation is equipped between inner cylinder 2 and shell 1
Channel 3,2 bottom activity of shell are equipped with stirring blade 8, which protrudes into inner cylinder 2, and 1 bottom surface attaching of shell has installation
Seat 16, mounting base is interior to be equipped with motor 12, and the drive shaft of motor 12 is equipped with swivel plate 11, between stirring blade 8 and swivel plate 11
Connected by magnetic force mechanism, 1 bottom surface of shell is equipped with the heater 9 connecting with control system, 1 side wall of shell be fixedly installed with
Temperature sensor 7, ultraviolet emissioning tube 13 and the fluorescence reception sensor 4 of control system connection, motor 12 and control system connect
It connects, ultraviolet emissioning tube is received by fluorescence reception sensor after reflection towards emitting ultraviolet light line in circulation canal, inner cylinder 2
Upper end is equipped with seal cover board 6, which covers 1 upper end of shell simultaneously, so that shell, inner cylinder and sealing
Enclosed construction is formed between cover board, is not easy by external interference.Temperature sensor is selectively mounted to outer casing underpart position, Neng Gougeng
Good detection temperature.The settable corresponding light pipe of ultraviolet emission pipe or light guide, ultraviolet light is guided to circulation canal
In can be radiated at testing liquid in circulation canal, then reflect and received by fluorescence reception sensor again, to read glimmering
Light value.
The circulation canal includes the interlayer being located between upper through-hole 5, inner cylinder and the outer housing of inner cylinder side wall upper part
Lower gap between channel and inner cylinder lower end and outer housing inner bottom surface, through-hole, sandwich passage and lower gap form feed flow on this
The circulation canal of body flowing, testing liquid flows to sandwich passage from inner cylinder lower end, then flow back into inner cylinder from upper through-hole.
The stirring blade 8 includes four blades, and a blade magnet 15, and phase are installed in the bottom surface of each blade
The polarity of adjacent blade magnet 15 is on the contrary, i.e. the pole S, the pole N are arranged alternately;Swivel plate 11 is equipped with adsorption magnet 10, adsorption magnet
10 quantity is identical as the quantity of blade magnet 15 and polarity is corresponding identical, settable four leaflet plates on same swivel plate,
It is corresponded with four blades, according to identical charges repel each other the principle that there is a natural attraction between the sexes, drives stirring blade rotation.Using on swivel plate
Adsorption magnet and blade magnet form the suction to attract each other.In order to meet adsorption capacity, the biggish magnet of magnetic force is selected.
In addition, 1 inner bottom surface of shell is equipped with rotor fixed position block 14, stirring blade 8 is actively plugged in the locating piece 14,
So that it is guaranteed that stability when stirring blade rotates.
The bottom surface of the seal cover board is equipped with step position, and the upper end of shell, which is supported, is mounted on the step position, lift-off seal
Energy.
In addition, the control system includes MCU main control module, and the ultraviolet light connecting respectively with the MCU main control module
Transmitting module, fluorescence detection module, heating module, temperature detecting module, rotation control module, man machine operation interface, communication connect
Mouthful and data storage center, heating module connect with heater, heater is usually heater strip, ultraviolet light emission module with it is ultraviolet
Transmitting tube connection, control ultraviolet emission pipe emit the ultraviolet light of same size.Fluorescence detection module then with fluorescence reception sensor
Connection receives fluorescence detection data.Temperature detecting module is connect with temperature sensor, is carried out to the temperature data detected defeated
It send.Rotation control module is then connected to motor, and controls the parameters such as power, the revolving speed of motor, it is ensured that motor maintains a setting
The range of speeds in.Operator can be inputted by man machine operation interface, while be communicated to connect using communication interface and other equipment.
Inner cylinder body sidewall is solid slab, and upper through-hole only is arranged in side wall upper part, so that it is guaranteed that testing liquid can lead to from upper
Hole flowing.Heater extraction wire connects to power supply.
A kind of control method of Portable fluorescence detection device, comprising the following steps:
Sample to be tested is added in inner cylinder and dissolution liquid, set temperature section, heater are begun to warm up, makes shell
Temperature is maintained in set temperature section.Record sample to be tested weight;Lysate specification;Lysate volume.Set examination criteria
Temperature;Heater strip is heated;Temperature sensor Real-time Feedback temperature.
Motor drives stirring blade rotation, stirs to form testing liquid to sample to be tested and dissolution liquid crushing, prepare liquid
Body flows in circulation canal to be uniformly distributed.High-speed stirred is crushed sample to be tested;Lysate is uniformly mixed with determinand.
Ultraviolet emission pipe emitting ultraviolet light line, reflects again after testing liquid is irradiated by ultraviolet light and is sensed by fluorescence reception
Device, which receives, reads fluorescent value.Only in a position setting ultraviolet emission pipe and fluorescence reception sensor, single-point detection is formed.It is ultraviolet
Light is by being radiated on testing liquid, and prepare liquid is rotated with balanced fixed speed, some fixed bit on the wall of test container
It sets, balanced sampling fluorescent value.
The fluorescent value read is sent to control system, analysis is compared with the fluorescent value of setting, obtains as a result, simultaneously
It is stored, is detected by multiple repairing weld, averaged, the fluorescence of as current sample to be tested.The sampling of fluorescence values
Rate is the 2 times or more of revolving speed;It is carried out by the numerical value of multiple repairing weld, Windows filter, count processing of averaging;Obtained numerical value
It is characterized as the fluorescence of sample to be tested.
It is described by temperature sensor real-time detection skin temperature, pass through control system and control the unlatching of heater, close
Or the adjustment of power.
It should be noted that these are only the preferred embodiment of the present invention, it is not intended to restrict the invention, although ginseng
According to embodiment, invention is explained in detail, for those skilled in the art, still can be to aforementioned reality
Technical solution documented by example is applied to modify or equivalent replacement of some of the technical features, but it is all in this hair
Within bright spirit and principle, any modification, equivalent replacement, improvement and so on should be included in protection scope of the present invention
Within.
Claims (10)
1. a kind of Portable fluorescence detection device, including control system and shell, which is characterized in that be equipped with inner cylinder in the shell
Body, inner cylinder are the hollow structure of both ends open, and circulation canal is equipped between inner cylinder and shell, and outer casing bottom activity is equipped with and stirs
Blade is mixed, which protrudes into inner cylinder, and enclosure bottom attaching has mounting base, is equipped with motor, the drive of motor in mounting base
Moving axis is equipped with swivel plate, is connected between stirring blade and swivel plate by magnetic force mechanism, and enclosure bottom is equipped with is with control
The heater of system connection, side wall of outer shell are equipped with temperature sensor, ultraviolet emissioning tube and the fluorescence reception connecting with control system
Sensor, motor are connect with control system, and ultraviolet emissioning tube is glimmering after reflection towards emitting ultraviolet light line in circulation canal
Optical receiving sensor receives.
2. Portable fluorescence detection device according to claim 1, which is characterized in that the circulation canal includes in being located at
Sandwich passage and inner cylinder lower end and outer housing inner bottom surface between the upper through-hole on cylinder lateral wall top, inner cylinder and outer housing it
Between lower gap, the circulation canal of through-hole on this, sandwich passage and the composition feed flow body flowing of lower gap.
3. Portable fluorescence detection device according to claim 2, which is characterized in that the inner cylinder upper end is equipped with close
Plate is covered, which covers upper shell simultaneously.
4. Portable fluorescence detection device according to claim 3, which is characterized in that the stirring blade includes at least two
A blade magnet is installed in the bottom surface of a blade, each blade, and the polarity of adjacent blade magnet is opposite;On swivel plate
Equipped with adsorption magnet, the quantity of adsorption magnet is identical as the quantity of blade magnet and polarity is corresponding identical.
5. Portable fluorescence detection device according to claim 4, which is characterized in that the swivel plate has leaflet plate,
The quantity of leaflet plate is equal with the quantity of the blade on stirring blade.
6. Portable fluorescence detection device according to claim 5, which is characterized in that the shell inner bottom surface is equipped with rotor
Locating piece.
7. Portable fluorescence detection device according to claim 6, which is characterized in that the bottom surface of the seal cover board is equipped with
Step position, the upper end of shell, which is supported, is mounted on the step position.
8. Portable fluorescence detection device described in any one of -7 according to claim 1, which is characterized in that the control system
Including MCU main control module, and the ultraviolet light emission module, the fluorescence detection module, heating that are connect respectively with the MCU main control module
Module, temperature detecting module, rotation control module, man machine operation interface, communication interface and data storage center.
9. a kind of control method of Portable fluorescence detection device, comprising the following steps:
Sample to be tested is added in inner cylinder and dissolution liquid, set temperature section, heater are begun to warm up, makes the temperature of shell
It is maintained in set temperature section;
Motor drives stirring blade rotation, stirs to form testing liquid to sample to be tested and dissolution liquid crushing, testing liquid exists
Flowing is uniformly distributed in circulation canal;
Ultraviolet emission pipe emitting ultraviolet light line, reflects again after testing liquid is irradiated by ultraviolet light and is connect by fluorescence reception sensor
It receives and reads fluorescent value;
The fluorescent value read is sent to control system, is compared analysis with the fluorescent value of setting, is obtained as a result, and carrying out
Storage, is detected, averaged, the fluorescence of as current sample to be tested by multiple repairing weld.
10. the control method of Portable fluorescence detection device according to claim 9, which is characterized in that described to pass through temperature
Sensor real-time detection skin temperature is spent, unlatching, closing or the adjustment of power of heater are controlled by control system.
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Cited By (2)
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CN111021108A (en) * | 2019-12-24 | 2020-04-17 | 绍兴市质量技术监督检测院 | Method for reducing or removing pentachlorophenol in polyester and polyester blended fabric |
CN113877666A (en) * | 2021-09-29 | 2022-01-04 | 广西埃索凯新材料科技有限公司 | Manganese dioxide and mixed broken system of coal that reduces |
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