CN203745471U - Colloidal gold immunoassay detection system based on image sensor - Google Patents
Colloidal gold immunoassay detection system based on image sensor Download PDFInfo
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Abstract
The utility model discloses a colloidal gold immunoassay detection system based on an image sensor, and belongs to the field of colloidal gold immunoassay. The detection system comprises a test strip detection platform, wherein the test strip detection platform is provided with a test strip arrangement platform for arranging a test strip; a lighting module and an imaging module are arranged above the test strip arrangement platform of the test strip detection platform; the lighting module consists of a lighting light source and a light homogenization unit arranged below the lighting light source; the lighting light source is a hollow annular light emitting diode array light source formed by uniformly distributing a plurality of light emitting diodes according to an annular array; the image sensor of the imaging module adopts a CMOS (complementary metal oxide semiconductor) image sensor; the signal output end of the imaging module is electrically connected with the input end of a data acquisition processing device. According to the system, an annular matrix is adopted for lighting and is matched with the light homogenization unit, so that output light is homogenized, and the detection result is accurate; the CMOS image sensor is adopted as the imaging module, so that the acquisition speed is high, and a requirement for dynamically tracking and observing a reaction process can be met.
Description
Technical Field
The utility model relates to a colloidal gold immunoassay detecting instrument field especially relates to and uses an instrument based on image sensor carries out colloidal gold immunoassay, can be used for the colloidal gold immunoassay's of fields such as biological detection technique, food safety inspection quantitative determination.
Background
The colloidal gold immunoassay has been developed in many fields such as drug detection, biomedicine and the like in recent years and receives more and more attention from related research fields, and a colloidal gold detection technology derived from the colloidal gold immunoassay has been developed correspondingly. In recent years, various domestic research institutions and companies are competing to research colloidal gold detection instruments.
In recent years, there are no more than two methods for collecting and processing the information of the colloidal gold test strip produced in the market. One is to adopt the motor to drive the photoelectric receiving arrangement of the linear array to carry on the line scanning to gather to the test paper strip, then send the point information gathered into the microprocessor one by one and store, process, so the measuring speed of the apparatus mainly depends on the speed that the motor scans, relatively slow, and the motor scanning mechanism is bulky; in order to solve the disadvantage of slow speed of a motor scanning mode, at present, a colloidal gold detector is provided which adopts an area array CCD to carry out area array photographing and acquisition on information of a test strip, then transmits acquired two-dimensional image information to the inside of a microprocessor to carry out data processing, and finally gives a measurement result.
However, although the speed of the colloidal gold detector adopting the area array CCD is higher than that of a motor scanning mode, the acquired information quantity is large, the CCD can only wait until all image information is acquired, and then the electric signal is amplified by the amplifying circuit and transmitted to the microprocessor, so that the problems of time waste and influence on the image acquisition speed exist, and the CCD, the additional driving circuit and the amplifying circuit thereof also increase the volume and the cost of the whole equipment. Because the immunochromatography reaction is a dynamic process, the color development of the colloidal gold also belongs to the dynamic process, and the prior art can not obtain an accurate detection result at a speed of the order of seconds, so that the tracking observation of the reaction process can not be realized, and the immunochromatography reaction process can not be studied more deeply. Even if the immunohistochemical reaction fails, the cause of the reaction failure cannot be analyzed well in the case where the detection speed is slow. In addition, in the prior art, in order to obtain the uniformity of illumination, the number of light sources is generally required, the number of light sources is increased from two diodes to four or even six diodes which are symmetrically arranged, but the uniformity of illumination cannot be ensured only by increasing the number of the light sources, and the problem that the accuracy of the collected image cannot be ensured due to uneven illumination exists.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to provide a colloidal gold immunoassay's detecting system based on image sensor, can solve current colloidal gold detector and can not accurately gather image data with second magnitude of speed, processing speed can't satisfy the problem of dynamic tracking observation reaction process.
In order to solve the above technical problem, the utility model provides a colloidal gold immunoassay's detecting system based on image sensor, include:
the test strip detection platform, the lighting module, the imaging module and the data acquisition and processing device are arranged on the test strip; wherein,
the test strip detection platform is provided with a test strip placement platform for placing test strips;
the lighting module and the imaging module are both arranged above a test strip placing platform of the test strip detection platform;
the illumination module consists of an illumination light source and a light homogenizing unit arranged below the illumination light source, wherein the illumination light source is a hollow annular light-emitting diode array light source formed by uniformly distributing a plurality of light-emitting diodes according to an annular array;
the image sensor of the imaging module adopts a CMOS image sensor, and the signal output end of the imaging module is electrically connected with the input end of the data acquisition and processing device.
Further, in the above detection system, the illumination light source is a hollow annular light emitting diode array light source formed by a plurality of light emitting diodes uniformly distributed in an annular array; the light homogenizing unit is a hollow annular structure matched with the shape of the illumination light source.
Furthermore, in the above detection system, the illumination light source employs 8 light emitting diodes with wavelength of 525nm, divergence angle of 180 degrees, light intensity of 500mcd and diameter of 3mm, and each light emitting diode employs a light emitting diode which is only output with radial light after shading treatment;
the light homogenizing unit adopts a homogenizing glass plate with one surface plated with an antireflection film and the other surface as a roughened surface and the central wavelength of 537 nm.
Further, in the above detection system, an imaging lens group is disposed at a front end of an image sensor of the imaging module, and the imaging lens group is disposed in a hollow portion of the illumination module.
Further, in the above detection system, the data acquisition and processing device includes:
the device comprises a microprocessor chip, a display device, an input device, a clock chip, a USB control chip and a USB interface; wherein,
the microprocessor chip is respectively and electrically connected with the display device, the input device, the clock chip and the USB control chip;
the USB control chip is electrically connected with the USB interface.
Furthermore, in the above detection system, the display device adopts an LCD display screen and is electrically connected to the microprocessor chip through a buffer.
Further, in the above detection system, the data acquisition and processing device further includes: a micro printer and a second buffer; the micro printer is electrically connected with the data acquisition and processing device through the second buffer.
Furthermore, in the above detection system, the data acquisition and processing device is electrically connected to the signal output terminal of the imaging module through a serial interface of a microprocessor chip of the data acquisition and processing device.
Further, in the above detection system, the microprocessor chip is a DSP microprocessor.
Further, the above detection system further includes: and the USB memory is electrically connected with the USB interface of the data acquisition and processing device.
The utility model has the advantages that: the illumination module of the detection system adopts an annular matrix illumination mode and is assisted by a light homogenizing unit, so that output light is more homogenized, the test strip illumination of the test strip to be detected is clearer, and the detection result is more accurate; the image sensor adopted by the imaging module adopts a CMOS image sensor, so that the acquisition speed is higher than that of a CCD, and the automatic gain, automatic white balance and large dynamic range are realized. In addition, the CMOS image sensor has small volume and low power consumption, so that the power consumption of the whole machine is low and the volume is small. The detection system has the advantages of simple structure, small volume, high image acquisition speed during detection, and capability of meeting the requirement of dynamically tracking and observing the reaction process by matching with a data acquisition and processing device.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the description below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a detection system for colloidal gold immunoassay based on an image sensor according to an embodiment of the present invention;
fig. 2 is a schematic view of an illumination module of a detection system according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a data acquisition and processing device of a detection system according to an embodiment of the present invention;
fig. 4 is a schematic view of a connection portion between a USB memory and a data acquisition and processing device of a detection system according to an embodiment of the present invention;
FIG. 5 is a working curve of the detection system provided in the embodiment of the present invention for plotting the T/C value measured after dropping HCG standard solution and the concentration gradient of the original sample;
fig. 6 is a working curve of the detection system provided by the embodiment of the present invention plotting the T/C value measured after the HBsAg standard solution is added dropwise and the concentration gradient of the original sample.
Detailed Description
The technical solutions in the embodiments of the present invention are described below clearly and completely, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiment of the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Fig. 1 shows that the embodiment of the present invention provides a detection system for gold immunoassay based on an image sensor, which includes: the test strip detection platform 1, the lighting module 2, the imaging module 3 and the data acquisition and processing device 4;
the test strip detection platform 1 is provided with a test strip placement platform 101 for placing test strips, and a colloidal gold test strip 102 to be detected can be inserted into the test strip placement platform 101 each time;
the lighting module 2 and the imaging module 3 are both arranged above the test strip placing platform 101 of the test strip detection platform 1, and the lighting end of the lighting module 2 and the image acquisition end of the imaging module 3 both correspond to the positions of the colloidal gold test strips 101 on the test strip placing platform 101 of the test strip detection platform 1;
the illumination module 2 is composed of an illumination light source 201 and a light homogenizing unit 202 arranged below the illumination light source 201, wherein the illumination light source 201 is a hollow annular light emitting diode array light source formed by uniformly distributing a plurality of light emitting diodes according to an annular array;
the image sensor of the imaging module 3 adopts a CMOS image sensor 301, and the signal output end of the imaging module 3 is electrically connected with the input end of the data acquisition and processing device 4.
As shown in fig. 2, the illumination module 2 of the above detection system is composed of an illumination light source 201 and a light uniformization unit 202 disposed below the illumination light source; the illumination light source 201 is a hollow annular light emitting diode array light source formed by uniformly distributing a plurality of light emitting diodes according to an annular array; the light homogenizing unit 202 is a hollow annular structure matched with the shape of the illumination light source, and the light homogenizing unit can further homogenize the obtained annular light source to obtain better uniform light. The illumination module formed by matching the hollow annular light-emitting diode array light source with the light homogenizing unit has good uniformity of illumination light, so that the accuracy of a measurement result is high, uniform light emitted by the annular array light source formed by the plurality of light-emitting diodes irradiates the surface of a tested paper strip, the working area of the test paper strip is illuminated in a diffuse reflection mode, the part which is difficult to see originally can be highlighted, and the final measurement result is more reliable. The utility model discloses an adopt the illumination module that the cooperation of cavity annular light homogenization unit of cavity annular light emitting diode array light source and setting formed, prior art has been overcome, for obtaining the homogeneity of illumination, generally only carry quantity requirement to the light source, increase to four or six diodes's symmetry even from two diodes and arrange, and only rely on the quantity that increases the light source, and can not improve illumination homogeneity, also can not shine the part that the test paper strip that is detected is difficult to see originally clearly, can't guarantee finally to obtain accurate image data's problem.
In the lighting module 2, 8 light emitting diodes with wavelength of 525nm, divergence angle of 180 degrees, light intensity of 500mcd and diameter of 3mm are adopted as lighting sources, and each light emitting diode adopts a light emitting diode which is only output with radial light after shading treatment; the light homogenizing unit adopts a homogenizing glass plate with one surface plated with an antireflection film and the other surface as a roughened surface and the central wavelength of 537nm, and the homogenizing glass plate can enable light to uniformly penetrate through.
In the above detection system, the front end of the CMOS image sensor 301 of the imaging module 3 (structure see fig. 1) is provided with an imaging lens group 302, and the imaging lens group 302 is disposed in the hollow portion of the illumination module 2. Through the arranged imaging lens group 302, under the irradiation of the illumination light source, after the colloidal gold test strip 101 information is projected onto the CMOS image sensor 301 through the imaging lens group, an optical signal is converted into an electric signal, and then the electric signal is output to the data acquisition and processing device 4 for information extraction and test strip concentration algorithm analysis, and finally an accurate measurement result is given.
As shown in fig. 3, in the above detection system, the data acquisition and processing device 4 includes:
a microprocessor chip 401, a display device 404, an input device 405, a clock chip 406, a USB control chip 407 and a USB interface 4070; wherein,
the microprocessor chip 401 is electrically connected with the display device 404, the input device 405, the clock chip 406 and the USB control chip 407 respectively;
the USB control chip 407 is electrically connected to the USB interface 4070.
In the data acquisition and processing device 4, the display device 404 is an LCD display screen and is electrically connected to the microprocessor chip 401 through the buffer 402.
The data acquisition and processing device 4 further includes: a micro printer 403 and a buffer two 4021; the micro printer 403 is electrically connected to the microprocessor chip 401 through the second buffer 4021.
The data acquisition and processing device is electrically connected with the signal output end of the imaging module 3 through the serial interface 408 of the microprocessor chip 401.
The microprocessor chip of the data acquisition and processing device 4 adopts a DSP microprocessor.
The above-mentioned detection system further comprises: the USB memory 5 is electrically connected to the USB interface 4070 of the data acquisition and processing device 4. Through the matching of the USB memory and the USB interface of the data acquisition and processing device, the reading and writing of the peripheral USB memory are realized, the data storage space of the whole system is improved, the storage of mass data can be realized, and the problems of low speed, low efficiency and inconvenience in processing large data volume in the data transmission of a serial interface are solved. The existing colloidal gold detecting instrument usually adopts an instrument memory to store detected colloidal gold detection data, the memory capacity is usually limited, if the detection quantity is increased, the storage requirement cannot be met, the colloidal gold detecting instrument needs to frequently transmit with an upper computer through a serial interface, the process is troublesome, and the transmission efficiency is low, so that the time is wasted. In addition, the measured image data sometimes needs to be stored first and then analyzed and researched, but the image data often occupies a large memory due to large storage capacity, so that a plurality of test strip images can occupy the memory, and then the test strip images are uploaded by virtue of a serial interface, so that the speed is very slow, and the subsequent detection and the detection efficiency are influenced. And the utility model discloses a set up USB control chip and USB interface in data acquisition and processing device, can connect outside USB memory, then fine overcome current colloidal gold detecting instrument's data storage capacity too little, can't realize mass storage's problem.
When the detection system works, under the illumination of the illumination module 2, the test strip 102 arranged on the test strip detection platform 1 forms an image on the imaging module 3, the imaging module 3 outputs an electric signal converted from an image to the data acquisition and processing device 4, and after the data acquisition and processing device 4 processes and analyzes the electric signal, the data to be stored is output to the USB memory 5 connected with the data acquisition and processing device 4 for storage.
The detection system of the present invention will be further explained with reference to the drawings and the specific embodiments.
Fig. 1 is a schematic structural diagram of the detection system of the colloidal gold immunoassay based on the image sensor of the present invention. As can be seen from fig. 1, the detection system comprises: the test strip detection platform 1, the lighting module 2, the imaging module 3, the data acquisition and processing device 4 and the USB memory 5;
the test strip detection platform is provided with a test strip placement platform 101, and the test strip placement platform 101 is provided with a test strip 102;
the illumination module 2 is composed of a hollow ring-shaped light emitting diode array light source as an illumination light source 201 and a light uniformizing unit 202. The parallel light emitted from the hollow ring-shaped led array light source as the illumination light source 201 is further homogenized after passing through the light uniformizing unit 102.
The imaging module 3 is composed of a CMOS image sensor 301 as an image sensor and an imaging lens group 302 disposed at the front end of the CMOS image sensor.
As shown in fig. 2, the hollow annular led array light source of the illumination module 2 as the illumination light source 201 is formed by a plurality of leds arranged in annular symmetry around the optical axis of the imaging lens group 302 of the imaging module 3 to obtain annular parallel light.
As shown in fig. 3, the data acquisition and processing device 4 includes a microprocessor chip DSP401, a buffer 402, an LCD screen 403, a micro printer 404, a keyboard 405, a clock chip 406, a USB control chip 407, and a serial interface 408 for communicating with the CMOS image sensor 301 of the imaging module 3.
As shown in fig. 4, in the detection system, the structure for implementing mass data storage by connecting the external USB memory 5 includes: the USB control chip 407 (formed by connecting the RAM buffer 4071 and the serial port transmission module 4072) connected to the microprocessor chip DSP401 and the USB interface 4070 (both of which form a USB control module), and the USB interface 4071 is connected to the external USB memory 5.
Utilize the utility model discloses a detecting system examines time measuring: after the colloidal gold test strip 102 dripped with the sample solution is fixed on the test strip placement platform 101, the illumination module 2 is used for illuminating the colloidal gold test strip 102 on the test strip placement platform 101, the imaging CMOS image sensor 301 of the imaging module 3 is used for detecting the colloidal gold test strip 102 to obtain image data of the colloidal gold test strip 102, then the data acquisition and processing device 4 is used for carrying out signal processing on the image data of the test strip to finally obtain the concentration of the dripped sample solution on the test strip, and the measured measurement result data can be stored through an external USB memory 5 (such as a U disk) connected with the data acquisition and processing device 4.
The test strip placement platform 101 is designed according to the geometric dimension of the test strip box to be tested, and finally, the test strip can be embedded on the platform, and the surface to be tested is exposed in the visual field of the imaging module 3.
The lighting module 2 is selected by firstly measuring the absorption spectrum of the colloidal gold solution to be measured by using a spectrophotometer to obtain the absorption peak and the absorption spectrum width of the colloidal gold solution to be measured. And designing a diode lighting source according to the measured absorption spectrum of the colloidal gold solution, so that the spectrum of the light source irradiating the colloidal gold marker to be measured is in the absorption spectrum range of the colloidal gold solution. In order to obtain better uniform light illumination, the illumination light source 201 adopts a plurality of light emitting diodes which are consistent with the absorption spectrum range of the colloidal gold solution to perform annular array arrangement, the arrangement structure is symmetrically and annularly arranged around the optical axis of the imaging lens group, and the light emitting brightness and the divergence angle of the light emitting diodes are moderate. In addition, the surface of the light emitting diode can be finely ground by adopting a professional mechanical process to obtain a proper light equalizing effect, and then the light homogenizing unit 202 is arranged to attenuate and weaken the intensity of parallel light, so that the luminosity is more uniform finally.
After the uniform light emitted from the lighting module 2 irradiates the colloidal gold test strip 101, each point of the colloidal gold test strip 101 absorbs light to different degrees, the light emitted from each point is captured on the photodiode of the CMOS image sensor 301 after passing through the imaging lens group 302, and an output current is generated, wherein the intensity of the output current is proportional to the light intensity information of the image on the colloidal gold test strip 101. According to the lambert beer law, the magnitude of the signal is exactly proportional to the concentration of the solution on the colloidal gold test strip. The electric signals are amplified by the amplifier in the CMOS image sensor 301, and then directly converted into corresponding digital signals by the a/D amplifier in the CMOS image sensor 301, and the digital signals are combined and then directly sent to the microprocessor chip DSP401 of the data acquisition and processing device 4 for image signal processing.
The microprocessor chip DSP401 of the data acquisition and processing device 5 is used as a core of data acquisition and signal processing of the whole system, and mainly commands the peripheral devices to execute corresponding functions by receiving commands from the keyboard 405, such as displaying content on the display device 404 (which may adopt an LCD screen), printing by the micro printer 403, acquiring data information of the colloidal gold test strip 101 through the serial interface 408, and transmitting the data to the USB memory 5 through the USB control chip 407.
The display device 404 is connected with the microprocessor chip DSP401 through the two buffers 402 to display a signal processing result in real time, and the display device 404 also restores the obtained image data into an image to be displayed for a detector to watch, so that the display device is intuitive and convenient. The micro printer 403 is also connected to the microprocessor chip DSP401 through two buffers 402, which facilitates the user to print the relevant measurement results. The clock chip 406 is connected with the microprocessor chip DSP401 through three I/O ports, and reads and writes time data in a serial reading and writing mode, and if system time is wrong, a user can timely modify the time data through a keyboard in real time. The measurement results stored in the internal data memory are transmitted to the USB memory 5 through the USB control chip 407, and thus to the computer.
The specific process of utilizing the USB memory to store the mass data comprises the following steps: firstly, under the control of the microprocessor chip DSP401, the CMOS image sensor 301 collects image data of the colloidal gold test strip 101, stores the image data into the memory of the microprocessor chip DSP401, and performs data signal processing and calculation, and the result is stored in the memory of the DSP401, and when the capacity increases to a certain extent, that is, the memory of the DSP401 cannot meet the storage requirement, the DSP401 will select to transmit the calculation result data in batch through the RAM buffer 4071 of the USB control chip, and finally enter the USB memory 5 through the serial port transmission module 4072 and the USB interface. If the image data is in question during the measurement process, the acquired image data can be saved in the USB memory 5 and then transmitted to the PC for data processing by the USB memory 5.
The utility model discloses detecting system's working process as follows:
after the detection system is powered on, a display device 404 (an LCD screen) displays prompt information of a user, the types of samples to be detected, namely, ID numbers of the samples, items to be detected, operators and positive and negative reference values are set through the input of a keyboard 405, detection results are stored, printed and checked, and meanwhile, the time of the system can be modified, a detection command can be started, and the like.
The sample solution to be tested is dripped onto the colloidal gold test strip 102, so that the antigen in the sample solution is fully combined with the antibody of the colloidal gold test strip 102, namely, after the color reaction is finished, the colloidal gold test strip 102 is placed on the test strip placement platform 101. Illuminating light source 201 (being hollow diode ring array light source), the parallel light beam that sends is behind light homogenization unit 202, and the facula that forms on colloidal gold test paper strip 102 surface is enough can cover the region under test of whole test paper strip to illumination is even, in order to guarantee the utility model discloses higher stability and sensitivity have. The colloidal gold test strip 102 has two zones, one is a monitoring zone (C zone) for testing whether the test strip is qualified or not, and the sample solution binding concentration of the zone is fixed and unchanged; the other is a detected band (T band), the color development degree of the band depends on the concentration of the sample solution, and the T/C is finally taken as the concentration value of the final sample.
Pressing the "detect" key on keyboard 405 initiates the image capture action. After the detected area of the colloidal gold test strip 102 absorbs the light from the lighting module 2, the detected area is instructed by the microprocessor chip DSP401, the imaging lens group 302 of the imaging module 2 images on the photosensitive element of the CMOS image sensor 301, the image light intensity information of the colloidal gold test strip 102 is converted into an electric signal, the size of the electric signal is in direct proportion to the size of absorbance, the image electric signal obtained by multiple times of imaging is collected by the microprocessor chip DSP401 and then stored in the internal data memory, the operation is carried out to obtain an average value, the data of an image is obtained, after the filtering processing is carried out on the data, the interpolation processing is carried out on the data, and the image is imaged on an LCD screen (namely, the display device 404; and then, carrying out data processing by using the average value image data, respectively drawing an absorbance value curve of each point in one-dimensional direction on the test strip on a display device 404 (an LCD screen) by taking X, Y as a horizontal coordinate and a vertical coordinate in real time, namely a curve with information of a T band and a C band, obtaining a T/C value, namely the concentration of the sample solution by a detection algorithm, and further accurately judging the content of the biomolecule to be detected contained in the sample solution, wherein the value can be compared with a negative and positive reference value of a corresponding type to obtain accurate negative and positive judgment. The determination result is sent to an internal data memory for storage, and the detection result can be output through the USB memory 5. Meanwhile, in the process of collecting image data, the collected large-capacity image data can be stored in the USB memory 5 through the USB controller 407 through key operation, so that the PC can reconstruct images, and further research is facilitated.
Example (b):
as shown in fig. 1, the illumination light source 201 in the illumination module 2 adopts 8 light emitting diodes with wavelength of 525nm, divergence angle of 180 degrees, light intensity of 500mcd and diameter of 3mm, which are arranged in an annular array, and then each diode is shielded to output only radial light, and then the light emitting diodes are symmetrically and annularly arranged around the optical axis of the imaging lens group. The light homogenizing unit 202 is a homogenizing glass plate, one surface of which is coated with an antireflection film, the center wavelength of which is 537nm, so that light is sufficiently transmitted, and the other surface of which is roughened and sufficiently scattered, and is arranged below the light emitting diode array 201, so that light with uniform brightness and intensity can be obtained on the test strip 102.
The CMOS image sensor 301 of the imaging module 3 can adopt OV528 serial interface transmission image sensor module produced by OmniVision company of America, the resolution VGA is 640 multiplied by 480, the transmission speed is 115200bit/s, the lens is 1/4', the uncompressed format output is directly output, the time waste caused by compression and decompression is reduced, and the CMOS image sensor has smaller volume and lower power consumption than a CCD. By adopting the novel CMOS image sensor, the image acquisition speed of the CMOS image sensor is higher than that of a CCD, the CMOS image sensor has automatic gain, automatic white balance and large dynamic range, and the detection efficiency can be effectively improved.
Standard sample i: HCG with the concentration of 400mIU/ml, 200mIU/ml, 100mIU/ml, 50mIU/ml and 25mIU/ml is adopted.
The standard sample II is 100ng/ml HBsAg with the concentration content of 100%, 50%, 25%, 12.5% and 6.25%.
The microprocessor chip DSP401 of the data acquisition processing device 4 can adopt a DSP chip MC56F8366 produced by Freescale company, is of a Harvard structure, supports parallel processing, has a dominant frequency of 60MHz, and achieves the execution speed of 60 million instructions; by adopting a multiple pipeline structure, the method can simultaneously complete the stages of reading the instruction, decoding the instruction, executing the instruction and the like; the chip is provided with a 512K program flash memory, a 4K program memory, an 8K data flash memory and a 32K data memory; 16A/D converters with 12 bits, two Serial Communication Interfaces (SCIs), 16 bit data lines and address lines, and a large number of I/O ports, which is favorable for the expansion of chips. The DSP microprocessor with extremely high processing speed is adopted to process image data and is matched with the CMOS image sensor with image acquisition speed higher than that of a CCD, so that the whole data acquisition and signal processing speed is higher than that of the prior art, and the high integration of the DSP chip saves a plurality of external expansion chips and further improves the integration level of the whole machine.
The USB controller 407 may use SL811HST manufactured by CYPRESS corporation, usa, and the USB memory 5 may use a 128M luck USB disk, USB1.1 transport protocol, or a USB memory with larger capacity using USB2.0 or USB3.0 transport protocol. Under the command of the DSP401, the USB flash disk 5 is subjected to batch write operation, the communication speed between the DSP chip 401 and the USB flash disk 5 can reach 150kbit/s by the USB flash disk 5, and finally, the mass storage of the measurement result is realized.
The detection system of the embodiment takes 6 seconds to finish one detection, which is superior to the detection speed of the existing CCD colloidal gold detector.
As shown in fig. 5 and 6, the curve is a working curve drawn by dropping two standard solution series of HCG and HBsAg, and the measured T/C value and the original sample concentration gradient, wherein the abscissa is the T/C value, the ordinate is the original sample concentration gradient, and the CV values of the two standard solution are both above 95%, which is superior to the prior art.
The detection system integrates all components and systems together, has small volume, has the volume of 195mm in width, 185mm in depth and 115mm in height, has the weight of less than 1.6kg, has the volume and the weight superior to those of the existing CCD colloidal gold detector, and can be carried to the field for field rapid detection.
The utility model discloses colloidal gold immunoassay detecting system based on CMOS image sensor to the color rendering characteristic of colloidal gold, adopt a plurality of emitting diode to be the annular and distribute, assist again with the light homogenization unit and carry out homogenization treatment to illumination, the specific lighting source of light source background noise of system has been reduced, and adopt CMOS image sensor as image receiving element and with the data acquisition processing apparatus cooperation that adopts microprocessor chip DSP, the detection speed has been improved, the consumption is reduced, the volume has been reduced. In addition, the data storage adopts a data acquisition processing device consisting of a DSP microprocessor to control a USB chip, and can be connected with an external high-capacity USB flash disk, so that the rapid mass data storage is realized, and the problems of low slow transmission speed and low efficiency of the original small memory and serial interface and an upper computer are solved.
The above description is only for the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are all covered by the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. An image sensor-based colloidal gold immunoassay detection system, comprising:
the test strip detection platform, the lighting module, the imaging module and the data acquisition and processing device are arranged on the test strip; wherein,
the test strip detection platform is provided with a test strip placement platform for placing test strips;
the lighting module and the imaging module are both arranged above a test strip placing platform of the test strip detection platform;
the illumination module consists of an illumination light source and a light homogenizing unit arranged below the illumination light source, wherein the illumination light source is a hollow annular light-emitting diode array light source formed by uniformly distributing a plurality of light-emitting diodes according to an annular array;
the image sensor of the imaging module adopts a CMOS image sensor, and the signal output end of the imaging module is electrically connected with the input end of the data acquisition and processing device.
2. The detection system for colloidal gold immunoassay based on an image sensor according to claim 1, wherein the light uniformizing unit is a hollow ring-shaped structure matched with the shape of the illumination light source.
3. The detection system for gold immunoassay based on an image sensor according to claim 2, wherein the illumination light source employs 8 light emitting diodes with wavelength of 525nm, divergence angle of 180 degrees, light intensity of 500mcd, diameter of 3mm, each light emitting diode employs a light emitting diode which is shielded to output only radial light;
the light homogenizing unit adopts a homogenizing glass plate with one surface plated with an antireflection film and the other surface as a roughened surface and the central wavelength of 537 nm.
4. The detection system for the image sensor-based colloidal gold immunoassay according to claim 1 or 2, wherein the front end of the image sensor of the imaging module is provided with an imaging lens group, and the imaging lens group is arranged in the hollow part of the illumination module.
5. The image sensor-based colloidal gold immunoassay detection system according to claim 1, wherein the data acquisition and processing device comprises:
the device comprises a microprocessor chip, a display device, an input device, a clock chip, a USB control chip and a USB interface; wherein,
the microprocessor chip is respectively and electrically connected with the display device, the input device, the clock chip and the USB control chip;
the USB control chip is electrically connected with the USB interface.
6. The system for detecting the gold immunoassay based on the image sensor as claimed in claim 5, wherein the display device employs an LCD display screen and is electrically connected with the microprocessor chip through a buffer.
7. The image sensor-based colloidal gold immunoassay detection system of claim 5, wherein the data acquisition and processing device further comprises: a micro printer and a second buffer; the micro printer is electrically connected with the data acquisition and processing device through the second buffer.
8. The detection system of the image sensor-based colloidal gold immunoassay of claim 5, wherein the data acquisition and processing device is electrically connected with the signal output end of the imaging module through a serial interface of a microprocessor chip of the data acquisition and processing device.
9. The detection system for colloidal gold immunoassay based on an image sensor according to claim 5 or 8, wherein the microprocessor chip employs a DSP microprocessor.
10. The detection system for image sensor-based colloidal gold immunoassay according to claim 1 or 5, further comprising: and the USB memory is electrically connected with the USB interface of the data acquisition and processing device.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103808918A (en) * | 2014-01-27 | 2014-05-21 | 上海耀研光电科技有限公司 | Image-sensor-based detection system of colloidal gold immunoassay |
| CN111795959A (en) * | 2020-07-24 | 2020-10-20 | 芯动森林(重庆)医疗科技有限公司 | Colloidal gold and fluorescence two-in-one data acquisition device and method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103808918A (en) * | 2014-01-27 | 2014-05-21 | 上海耀研光电科技有限公司 | Image-sensor-based detection system of colloidal gold immunoassay |
| CN111795959A (en) * | 2020-07-24 | 2020-10-20 | 芯动森林(重庆)医疗科技有限公司 | Colloidal gold and fluorescence two-in-one data acquisition device and method |
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