CN209069817U - Corn fruit biological information detection device - Google Patents

Abstract

Corn fruit biological information detection device, it is related to technical field of agricultural product detection, to solve the problems, such as that the information detector of existing corn fruit has a single function, the multiple detection devices of measuring multiple parameters need lead to equipment complicated composition, take up a large area, the present apparatus includes microscope, microscopical objective table is provided with cavity, cavity two relative side is respectively equipped with the channel being connected to objective table outside, further includes spectrometer, light source, detection fiber and gravity sensor；Sensing groove is provided in detection fiber, sensing groove and liquid to be detected are placed in cavity, the nanometer metallic film on sensing groove surface immerses in liquid to be detected, the emergent light of light source is incident to one end of detection fiber, the light of detection fiber other end outgoing is incident to spectrometer, gravity sensor is used to detect the measured object weight of stage surface, and microscopical camera lens is electron microscopic camera lens；Present apparatus purposes is the detection for the image information collecting of corn fruit, weight, sugar content and content of starch.

Description

Corn fruit biological information detection device

Technical field

The utility model relates to technical field of agricultural product detection, are in particular corn fruit biological information detection skills Art.

Background technique

Generally need to detect following several information to the detection of corn fruits and seeds, such as: fruit image, fruit weight, The information such as sugar content and amount of starch in fruit extracting solution require individually to fill for the detection of every kind of information in the prior art It sets；

Publication No. CN202393697U, the utility model patent that publication date is 2012.08.22, provide entitled " a kind of image collecting device of the detection of agricultural products based on machine vision ", the device include lighttight cabinet, case when taking pictures It is provided with the objective table of transparent material in vivo, cabinet is interior to be provided with video camera in the side of objective table, is provided with and is used in cabinet Reflection source used in reflected image is shot, is provided in cabinet in the other side of objective table and is used for what video camera was oppositely arranged The transmitted light source of agricultural product image is transmitted, which acquires the reflectogram of reflection agricultural product external feature information by reflection source Picture acquires the transmission image of reflection agricultural product internal characteristics information by transmitted light source, by the reflection for the agricultural product that will be acquired Image and transmission image combine, and obtain agricultural product image information；

Publication No. CN206683750U, the utility model patent that publication date is 2017.11.28, provide entitled " a kind of agricultural product Weight detecting device ", the device include briquetting, warning light, workbench, bull stick, placement plate, the first spring, Elevating lever, switch, second spring, the first mounting blocks, strikes block etc. at oscillating rod；Bench-top is connected with the first spring, the first bullet Spring is located at right above the first aperture, and the first spring upper end is connected with placement plate, and placement plate bottom is connected with elevating lever；

Publication No. CN85200042, the utility model patent that publication date is 1985.12.20 are provided entitled " sharp Light sugar-inspection apparatus ", the instrument be using polarimetry in sugar industry liquid glucose and fruit do sugar content detection instrument, the device Light source is done using gas laser, the big prepare liquid of chromatic value is contained by thin coupon, uses high precision angle-measuring device angle measurement；It carries out The sugar content of product detects；

Publication No. CNCN201628681U, the utility model patent that publication date is 2010.11.10, provide title For " a kind of Amylose starch detector ", which includes: laser emitter, photodetector unit, sample injector, simulation input mould Block and control unit；Wherein: the solution of pretreated detection is housed in the sample injector；The sample injector sends out the laser The laser that emitter issues is converted to optical signal, and the optical signal is delivered to the photodetector unit；The light detection list Member is supplied to the analog input module after the optical signal is converted to electric signal, is converted to number by the analog input module Described control unit is sent to after word signal；Described control unit is calculated according to the relational expression between absorbance and detection voltage Obtain amylose content；

Therefore can be summarized as in the prior art to the biological information of corn fruit and seed such as: Image Acquisition, weight measurement, The sugar content and amount of starch of fruit need to measure by each self-test device respectively, and each apparatus function is single, Duo Gejian Surveying device leads to equipment complicated composition, takes up a large area；

Existing detection device function list is had the following deficiencies: to the infomation detection of corn fruit and seed in the prior art One, measuring multiple parameters need multiple detection devices, and multiple detection devices lead to equipment complicated composition, take up a large area.

Utility model content

The utility model is the existing corn fruit of solution and the information detector of seed has a single function, measuring multiple parameters need The problem of multiple detection devices, multiple detection devices lead to equipment complicated composition, take up a large area.

To achieve the above object, the utility model provides corn fruit biological information detection device, including microscope 4, Cavity 4-1-1 is provided in the objective table 4-1 of microscope 4, cavity 4-1-1 two relative side is respectively equipped with a channel, described logical Road can will be connected to outside cavity 4-1-1 and objective table 4-1, liquid to be detected is filled in cavity 4-1-1, liquid to be detected is corn The biology of fruit extracts detection liquid,

Corn fruit biological information detection device further includes spectrometer 1, light source 2, detection fiber 3 and gravity sensor 4-2；

Detection fiber 3 is that can be realized SPR (Surface Plasmon Resonance, show plasma resonance) now The optical fiber of elephant, sensing groove closed at both ends is provided in detection fiber 3, and sensing groove bottom is fibre core 3-1, the surface fibre core 3-1 It is coated with nanometer metallic film 3-2, nanometer metallic film 3-2 is the metallic film that can excite SPR phenomenon, the biography of detection fiber 3 Sense groove is located in cavity 4-1-1, and guarantees that sensing groove is completely immersed in liquid to be detected, and one end of detection fiber 3 is from one Channel is extend out to outside objective table 4-1, and the other end of detection fiber 3 is extend out to outside objective table 4-1 from another channel,

The emergent light of light source 2 is incident to one end of detection fiber 3, and the light of 3 other end of detection fiber outgoing is incident to spectrum Instrument 1,

Gravity sensor 4-2 is used to detect the measured object weight on the surface objective table 4-1,

The camera lens of microscope 4 is electron microscopic camera lens 5.

The utility model device comparison prior art has the following beneficial effects: that 1. pass through utility model device, will be former The function integrated optimization of four sets of detection devices is a set of detection device, and the image of corn fruit and seed can be realized in set of device Acquisition, the detection of weight detecting, the sugar content in fruits and seeds extracting solution and amount of starch, are integrated with function, improve device Function it is rich；2. simplifying apparatus structure by the utility model；3. the device is a set of by being integrated into for former four covering devices Device, reduced footprint to original 30%.

Detailed description of the invention

Fig. 1 is the structural schematic diagram of corn fruit biological information detection device；

In figure: 1. spectrometers；1-1. multimode fibre；2. light source；2-1. single-mode tail fiber；3. detection fiber；4. microscope；4- 1. objective table；4-1-1. cavity；4-1-2. piston；4-2. gravity sensor；5. electron microscopic camera lens.

Fig. 2 is the structural schematic diagram of objective table；

In figure: 4-1. objective table；4-1-1. cavity；4-1-2. piston.

Fig. 3 is the structural schematic diagram that detection fiber passes through cavity state；

In figure: 1-1. multimode fibre；2-1. single-mode tail fiber；3. detection fiber；4-1. objective table；4-1-1. cavity；4-1- 2. piston.

Fig. 4 is the schematic diagram that cavity was connected to micro syringe pump and waste liquid pool and was added detection liquid status；

In figure: 1-1. multimode fibre；2-1. single-mode tail fiber；3. detection fiber；4-1. objective table；4-1-1. cavity；4-1- 2. piston；6. waste liquid pool；7. micro syringe pump.

Fig. 5 is the structural schematic diagram that detection fiber has sensing groove；

In figure: 3. detection fibers；3-1. fibre core；3-2. nanometer metallic film.

Fig. 6 is the electrical connection schematic diagram of gravity sensor；

Fig. 7 is the electrical connection schematic diagram of computer and wireless transmitter；

Fig. 8 is the structural schematic diagram of detection processing chip；

Fig. 9 is the structural schematic diagram of switch；

Figure 10 is the structural schematic diagram of reset key；

Figure 11 is the structural schematic diagram of ZigBee I；

Figure 12 is the structural schematic diagram of gravity sensor；

Figure 13 is the structural schematic diagram of display screen；

Figure 14 is the structural schematic diagram of conversion circuit.

Specific embodiment

Specific embodiment one illustrates present embodiment below with reference to Fig. 1-14, and the utility model is to solve existing corn The information detector of fruit and seed has a single function, measuring multiple parameters need multiple detection devices, and multiple detection devices cause to set Standby complicated composition, the problem of taking up a large area.

To achieve the above object, the utility model provides corn fruit biological information detection device, including microscope 4, Cavity 4-1-1 is provided in the objective table 4-1 of microscope 4, cavity 4-1-1 two relative side is respectively equipped with a channel, described logical Road can will be connected to outside cavity 4-1-1 and objective table 4-1, liquid to be detected is filled in cavity 4-1-1, liquid to be detected is corn The biology of fruit extracts detection liquid, and two channels of cavity 4-1-1 can be used piston with holes and block, and the two of detection fiber 3 End penetrates and is pierced by from the hole of two pistons respectively, in this way convenient for test, in order to avoid prepare liquid is caused to waste；

Corn fruit biological information detection device further includes spectrometer 1, light source 2, detection fiber 3 and gravity sensor 4-2；

Detection fiber 3 is the optical fiber that can be realized SPR phenomenon, and sensing groove closed at both ends is provided in detection fiber 3, is passed Feeling groove can be made using etch or side throwing method, and sensing groove bottom is fibre core 3-1, and the surface fibre core 3-1 is coated with nanogold Belong to film 3-2, nanometer metallic film 3-2 is the metallic film that can excite SPR phenomenon, and the sensing groove of detection fiber 3 is located at In cavity 4-1-1, and guarantee that sensing groove is completely immersed in liquid to be detected, one end of detection fiber 3 is extend out to from a channel Outside objective table 4-1, the other end of detection fiber 3 is extend out to outside objective table 4-1 from another channel,

The emergent light of light source 2 is incident to one end of detection fiber 3, and the light of 3 other end of detection fiber outgoing is incident to spectrum Instrument 1,

Gravity sensor 4-2 is used to detect the measured object weight on the surface objective table 4-1,

The camera lens of microscope 4 is electron microscopic camera lens 5.

The optional model that each device uses is illustrated as follows: spectrometer 1 is 350nm- for AQ6370C spectrum acquisition range The spectrometer of 1200nm, the AQ6370C spectrometer of Japanese Yokogawa, spectrum acquisition range are 350nm to 1200nm, and SPR is effective Spectral region is 400nm-1100nm, and selected spectrometer includes that the effective spectral range of SPR is ready to receive to complete SPR sensorgram Spectrum, light source 2 are the super continuum source that super continuum source is SuperK compact spectral width 400nm -2400nm, The production of NKT Photonics company, Denmark, the effective spectral range of light source 2 are 450nm-1100nm, and experiment shows only wavelength Light of the range within the scope of 450nm-1100nm can excite SPR phenomenon, and electron microscopic camera lens 5 is the electronics of 3R-WDKMC01 Microlens；

Connection relationship, the positional relationship of gravity sensor 4-2 provides following example:

Gravity sensor 4-2 detection includes detection processing chip, power supply, conversion circuit, switch, reset key, gravity biography Sensor, display screen and wireless communication device；

Positional relationship: detection processing chip, conversion circuit, gravity sensor 4-2 and wireless communication device can be located at loading In platform 4-1, switch, reset key and display screen can be located at stage surface, and gravity sensor 4-2 is placed in microscope 4 and acquires jade The underface on the surface for the objective table 4-1 that rice cracker uses in real time, gravity sensor 4-2 are used to detect the quilt on the surface objective table 4-1 Survey object weight；

Connection relationship: the energy output end of the power supply of microscope 4 and the energy input end of conversion circuit connect, conversion circuit Energy output end connect with the energy input end of detection processing chip, the energy output end of conversion circuit and gravity sensor 4- 2 energy input end connection, the energy output end of conversion circuit and the energy input end of display screen connect, the energy of conversion circuit The connection of the energy input end of output end and wireless communication device；

The signal output end of switch is connect with the signal input part of detection processing chip, the signal output end of reset key with The signal input part of detection processing chip connects, and the signal of the signal output end and detection processing chip of gravity sensor 4-2 is defeated Enter end connection, the signal output end of detection processing chip and the signal input part of display screen connect, the signal of wireless communication device Input terminal is connect with the signal output end of detection processing chip, the signal output end of wireless communication device and the letter of detection processing core The connection of number input terminal；

Chip or number of element types are chosen: for circuit connection diagram therein as shown in Fig. 6-14, detection processing chip is AT89C51 Single-chip microcontroller, it by the power conversion of microscope 4 is voltage available that power supply, which is the power supply of microscope 4, conversion circuit, is adopted in conversion circuit With LM7805 voltage stabilizing chip, line voltage is converted to the 5V voltage that in circuit and chip needs, switch by LM7805 voltage stabilizing chip Switched using MS-666 self-locking type, self-lock switch be when by order its closure when, state locking, until press again, state It just changes again, otherwise state saves always；Reset key is 1 self-locking key of cloud brightness, and self-locking key is when down state is locked Fixed, until pressing again, state is just changed again, and otherwise state saves always；Gravity sensor 4-2 uses A201S resistance Formula pressure sensor, the pressure for being applied to colorimetric sensor films region can be converted into the variation of resistance value, and pressure is bigger, and resistance is got over It is low；There is viscose glue at the sensor back side, sensor can be pasted onto detected position by the viscose glue protective film that need to only tear, and use is non- It is often easy, selection investigative range is the pressure sensor of 0-1lbs (4.4N), because of the light weight of corn fruits and seeds, is chosen Investigative range it is small detection accuracy can be improved；Display screen is four digital display tubes, in order to which real-time display measures corn fruit The weight of reality or seed, wireless communication device can be communicated with computer, and the weight of the corn seed of real-time measurement is passed It gives computer and carries out preservation statistics；

Choose the pin connection relationship of the component of model: the P1.0 pin and the signal of switch 4-2 of AT89C51 single-chip microcontroller are defeated Outlet connection, the P1.1 pin of AT89C51 single-chip microcontroller are connect with the signal output end of reset key 4-3, AT89C51 single-chip microcontroller P2.0 pin is connect with the output end of pressure sensor 13；A, B, C, D, E, F, G, DP pin of four digital display tubes successively with P0.0 to the P0.7 pin of AT89C51 single-chip microcontroller connects, and the piece of four digital display tubes selects 1,2,3,4 successively mono- with AT89C51 P2.1 to the P2.4 pin of piece machine connects, which charactron of four digital display tubes of P2.1-P2.2 pin control of AT89C51 is aobvious Show, the P0.0-P0.7 pin of AT89C51 single-chip microcontroller control the display numeral method what；

Its wireless communication device can be ZigBee device, and ZigBee device includes ZigBee I and ZigBee II, Zigbee I Pass through wireless connection with Zigbee II；

The signal input part of ZigBee I is connect with the signal output end of detection processing chip, the signal output end of ZigBee I It is connect with the signal input part of detection processing chip；ZigBee I is located in objective table 4-1.

The signal input part of ZigBee II is connect with the signal output end of computer disposal chip, and the signal of ZigBee II is defeated Outlet is connect with the signal input part of computer disposal chip；

Zigbee I and Zigbee II chooses CC2530Zigbee module；

The P3.1 pin of AT89C51 single-chip microcontroller is connect with the RXD pin of Zigbee I, the P3.0 pin of AT89C51 single-chip microcontroller It is connect with the TXD pin of Zigbee I；

The RXD pin of Zigbee I receives information, the P3.1 pin transmitting information of AT89C51 single-chip microcontroller；The TXD of Zigbee I Pin transmitting information, the P3.0 pin of AT89C51 single-chip microcontroller receive information, and the information mutually transmitted is corn fruit or kind The weight information of son.

Specific embodiment two illustrates present embodiment, present embodiment and specific embodiment below with reference to Fig. 1-14 The bottom cavity 4-1-1 unlike one is provided with apocenosis passage, the lower space connection of apocenosis passage and objective table 4-1, Apocenosis passage is equipped with piston 4-1-2, and piston 4-1-2 is for blocking apocenosis passage；

The apocenosis passage is used to arrange the residual liquid of light cavity 4-1-1 after the completion of being used to detect, it is convenient when blowdown to clean, no Apocenosis passage is blocked by piston 4-1-2 using when apocenosis passage, unplugs piston 4-1-2 using when apocenosis passage；

Apocenosis passage can also be used for the drying of cavity 4-1-1.

Specific embodiment three illustrates present embodiment, present embodiment and specific embodiment below with reference to Fig. 1-14 The device unlike one of one to two further includes micro syringe pump 7 and waste liquid pool 6, and prepare liquid is injected cavity 4- by micro syringe pump 7 In 1-1, extra prepare liquid flows into waste liquid pool 6；

The piston that two holes of band can be used in two channels of cavity 4-1-1 is blocked, and the both ends of detection fiber 3 are respectively from two It penetrates and is pierced by one hole of a piston, the output end of micro syringe pump 7 and the inflow end of waste liquid pool 6 are respectively from other two hole In penetrate and be pierced by, in this way convenient for test, in order to avoid prepare liquid is caused to waste；

Micro syringe pump 7 is the medical micro syringe pump of LSP01-1A, and micro syringe pump 7 passes through the one of cavity by detection liquid output end A channel transports to corn juice extracting solution to be detected in cavity, and the detection liquid output end of micro syringe pump 7 constantly pressurizes cavity 4-1-1 filling is full, and then the prepare liquid detected passes through another channel of cavity by the stream of waste liquid pool 6 under the effect of the pressure Enter end to flow into waste liquid pool 6；

Convenient for injecting prepare liquid into cavity 4-1-1, the increase of waste liquid pool 6 is convenient for from cavity 4- for the increase of micro syringe pump 7 Prepare liquid is discharged in 1-1, reduces operation difficulty.

Specific embodiment four illustrates present embodiment, present embodiment and specific embodiment below with reference to Fig. 1-14 The corn fruit biological information detection device further includes computer unlike one of one to three, electron microscopic camera lens 5 The connection of the picture signal input terminal of image signal output end and computer；

The addition of computer stores compared with convenient for data, is convenient for big data analysis.

Specific embodiment five illustrates present embodiment, present embodiment and specific embodiment below with reference to Fig. 1-14 The emergent light of the light source 2 unlike one of one to four is incident by the light that single-mode tail fiber 2-1 is incident on detection fiber 3 End, the emergent light of detection fiber 3 are incident on the optical receiving end of spectrometer 1 by multimode fibre 1-1；

Because of only one mode of the light of transmission in single-mode tail fiber 2-1, therefore interference is less, and the incoming detection of light source 2 can be improved The energy of optical fiber 3；

Multimode fibre 1-1 be step multimode fibre or gradual change multimode fibre, multimode fibre receive SPR sensorgram spectroscopic capabilities compared with By force, therefore multimode fibre 1-1 uses multimode fibre to be conducive to sense the reception of spectrum, improves the integrality of SPR sensorgram spectrum.

Specific embodiment six illustrates present embodiment, present embodiment and specific embodiment below with reference to Fig. 1-14 The detection fiber 3 unlike one of one to five is step optical fiber, gradual change multimode fibre or single mode optical fiber；

Step optical fiber, gradual change multimode fibre or single mode optical fiber etc. can be employed as the optical fiber of spr sensor；It can also be plastic light Fibre etc.；

But step optical fiber, gradual change multimode fibre or single mode optical fiber are to generate SPR phenomenon using more optical fiber, and price is low It is honest and clean, it is easy to use, it is convenient and economical and practical that test is carried out by these three optical fiber.

Specific embodiment seven illustrates present embodiment, present embodiment and specific embodiment below with reference to Fig. 1-14 The nanometer metallic film 3-2 unlike one of one to six is golden film or silverskin；

Golden film or silverskin are the metallic film of more commonly used excitation SPR phenomenon, and two kinds of films are easy to use, cost phase To cheap；

And golden film or silverskin with a thickness of 50nm, experiment shows when golden film or silverskin are with a thickness of 50nm-55nm as SPR The preferred thickness of the nano-gold film of sensing, the SPR sensorgram spectrum obtained in this case is clear, and the interference for sensing spectrum is less.

Specific embodiment eight illustrates present embodiment, present embodiment and specific embodiment below with reference to Fig. 1-14 The light that the light source 2 unlike one of one to seven is emitted is the white light of 450nm-1100nm wave band；

Because the effective optical band that can excite SPR phenomenon is 450nm-1100nm, improved using the light source 2 of this wave band The service efficiency of light source 2.

Specific embodiment nine illustrates present embodiment, present embodiment and specific embodiment below with reference to Fig. 1-14 The cavity 4-1-1 unlike one of one to eight is cuboid cavity；

The setting of cuboid cavity is in order to store enough prepare liquids, so that liquid to be detected sets the sensing with detection fiber 3 Groove and nanometer metallic film 3-2 come into full contact with, and are conducive to the accurate of testing result.

Specific embodiment ten illustrates present embodiment, present embodiment and specific embodiment below with reference to Fig. 1-14 The manufacturing process of the device unlike one of one to nine:

The detection fiber 3 for taking one section of 30cm-50cm is cut using two fiber end faces that optical fiber cutter will test optical fiber 3 It is flat, and dip alcohol with non-woven fabrics and wiped clean is carried out to two fiber end faces, the interlude 1cm long that will test optical fiber 3 is used up Fibre pincers strip coat, and it is clean with non-woven fabrics to dip alcohol wipe, by hydrofluoric acid etch (or side throwing method) by optical fiber Covering removal is until with fibre core 3-1 interface, corrosion process are as follows: the interlude 1cm for the detection fiber 3 handled well is attached to plastics Glass slide on, be placed under microscope, observe fibre core, and instill a few drop hydrofluoric acid, pass through the microscope moment observation corrosion feelings Condition stops when eroding to interface of the covering with fibre core 3-1, takes out, and sensing groove completes, and is rinsed with water hydrofluoric acid； It will test optical fiber 3 to be placed in compact particle sputter, sensing groove is placed in immediately below gold target, is first vacuumized, by adjusting plated film Time and plating membrance current take out the golden film of sensing groove plating 50nm thickness, the measurement and calibration of film thickness pass through three-dimensional shaped after plating is good The calibration of looks analyzer, detection sensitive zones complete；

It will test optical fiber 3 and pass through cavity 4-1-1 by two channels, one end of detection fiber 3 passes through there are two a bands One of hole of the piston in hole, another hole of the piston penetrate the output end of micro syringe pump 7 to be placed in cavity, will This piston fastens the channel；The other end of detection fiber 3 passes through another band, and there are two one of hole of the piston in hole, the work Another hole of plug penetrates the inflow end of waste liquid pool 6 to be placed in cavity, this piston is fastened the channel；Cavity 4-1-1 Bottom is provided with apocenosis passage, and apocenosis passage and the external of the bottom objective table 4-1 are connected to, by piston 4-1-2 by apocenosis passage bottom End separates with external；

Detection device completes；

The course of work and principle:

Corn fruit Image Acquisition:

Corn Fruit types are various, and not only type difference has more difference, and same kind is affected by environment also very big, therefore It needs to carry out Image Acquisition to it；By the plant-grid connection commercial power socket of microscope 4, corn fruit is placed on objective table 4-1, is led to The image information of the object lens acquisition corn fruit for the electron microscopic camera lens 5 being connected on microscope 4 is crossed, and sends information to meter Calculation machine chooses suitable corn by mouse confirmation by adjusting the position of the objective table 4-1 of microscope 4 and the depth of field of object lens The image information of fruit, and save；

Corn fruit Image Acquisition be conducive to later convenient for the corn fruit of identical type different times, different geographical, Growing state under varying environment is recorded and is compared, and can also equally be compared to different types of corn fruit；

Power supply and socket are disconnected using finishing；

The detection of corn fruit weight:

Step 1: power supply is converted the alternating current of 220V by conversion circuit by the plant-grid connection commercial power socket of microscope 4 For chip can voltage, and voltage available is respectively supplied to each chip；

Step 2: switch is placed in the position of closure, switch as self-lock switch, i.e., when by order its closure when, status lock Fixed, until pressing again, state is just changed again, and the P1.0 pin of AT89C51 single-chip microcontroller recognizes the signal intensity of switch, Therefore the pressure of AT89C51 single-chip microcontroller P2.0 pin identification gravity sensor 4-2 detection；

Step 3: pressing reset key, the height of the P1.1 pin identification reset key signal of AT89C51 single-chip microcontroller becomes Change, therefore the calculation of pressure that AT89C51 single-chip microcontroller detects gravity sensor 4-2 at this time is 0, and controls display screen and show, The numeral method of P2.1-P2.2 pin four digital display tubes of control of AT89C51, the P0.0-P0.7 of AT89C51 single-chip microcontroller draw Foot control system shows numeral method；

Step 4: corn fruit is placed in objective table 4-1, AT89C51 single-chip microcontroller P2.0 pin identifies pressure sensor 13 The pressure of detection shows the weight of corn on display screen 4-4, four digital-scroll techniques of P2.1-P2.2 pin control of AT89C51 Which numeral method of pipe, the P0.0-P0.7 pin of AT89C51 single-chip microcontroller control the display numeral method what；At this time If thinking, record record with pen the weight of the seed；

The P3.0 pin of AT89C51 single-chip microcontroller is connect with the TXD pin of Zigbee I, the P3.0 pin of AT89C51 single-chip microcontroller The weight information of corn fruit is sent to the RXD of Zigbee II by control Zigbee I, and the RXD pin of Zigbee II will receive The weight information of corn fruit be sent to computer disposal chip, saved；

Switch is disconnected the power supply of microscope 4 and commercial power socket again by its disconnection of ordering after detection；

Sugar content detection:

Spectrometer 1, light source 2 are connected with the power supply of micro syringe pump 7, the light of light source 2 is by single-mode tail fiber 2-1 injection detection In optical fiber 3, light excites SPR phenomenon by sensing groove, generates detection spectrum, detects in spectrum injection reception optical fiber 1-1, finally Detection spectrum received by spectrometer 1；The extracting solution for producing corn juice sugar content detection to be detected, the corn sugar content of fruit is examined The extracting solution of survey is placed in the injection-tube of micro syringe pump 7, and micro syringe pump 7 is by the output end of detection liquid by corn juice to be detected Extracting solution is injected in cavity 4-1-1 by a channel, and the detection liquid output end of micro syringe pump 7 constantly pressurizes cavity 4-1-1 Filling is full, and then the corn juice extracting solution detected is flowed under the effect of the pressure in waste liquid pool 6；

Light can generate evanescent wave in the interface of fibre core 3-1 and nanometer metallic film 3-2 when being transmitted to sensing groove, The light of a certain frequency of evanescent wave is consistent with the frequency of the free electron of nanometer metallic film 3-2 to resonate, the resonant frequency The energy of light absorbed by free electron, energy is substantially decayed, SPR resonance spectrum is generated under the action of corn juice extracting solution, The frequency of resonance spectrum is related with the refractive index of corn juice extracting solution, and the sugar content of corn juice extracting solution is different, then corn juice mentions Taking the characteristics of liquids such as refractive index of liquid will change, therefore influence the frequency of the light to resonate with free electron, therefore sense The resonance wave trough position of spectrum changes, therefore the detection spectrum received in spectrometer 1 changes, the position for the trough that resonates It changes, completes the sugar content detection of corn juice extracting solution；

It is compared with the standard spectrum of the sugar content of the spectrum kind corn fruit detected, that is, may know that out the jade The sugar content of rice juice extracting solution；

Detection, which finishes, disconnects the power supply of spectrometer 1, light source 2 and micro syringe pump 3；

Amount of starch detection:

Spectrometer 1, light source 2 are connected with the power supply of micro syringe pump 7, the light of light source 2 is by single-mode tail fiber 2-1 injection detection In optical fiber 3, light excites SPR phenomenon by sensing groove, generates detection spectrum, detects in spectrum injection reception optical fiber 1-1, finally Detection spectrum received by spectrometer 1；The extracting solution for producing corn juice amount of starch detection to be detected, corn fruit amount of starch is examined The extracting solution of survey is placed in the injection-tube of micro syringe pump 7, and micro syringe pump 7 is by the output end of detection liquid by corn juice to be detected Extracting solution is injected in cavity 4-1-1 by a channel, and the detection liquid output end of micro syringe pump 7 constantly pressurizes cavity 4-1-1 Filling is full, and then the corn juice extracting solution detected is flowed under the effect of the pressure in waste liquid pool 6；

Light can generate evanescent wave in the interface of fibre core 3-1 and nanometer metallic film 3-2 when being transmitted to sensing groove, The light of a certain frequency of evanescent wave is consistent with the frequency of the free electron of nanometer metallic film 3-2 to resonate, the resonant frequency The energy of light absorbed by free electron, energy is substantially decayed, SPR resonance spectrum is generated under the action of corn juice extracting solution, The frequency of resonance spectrum is related with the refractive index of corn juice extracting solution, and the amount of starch of corn juice extracting solution is different, then corn juice mentions Taking the characteristics of liquids such as refractive index of liquid will change, therefore influence the frequency of the light to resonate with free electron, therefore sense The resonance wave trough position of spectrum changes, therefore the detection spectrum received in spectrometer 1 changes, the position for the trough that resonates It changes, completes the amount of starch detection of corn juice extracting solution；

It is compared with the standard spectrum of the amount of starch of the spectrum kind corn fruit detected, that is, may know that out the jade The amount of starch of rice juice extracting solution；

Detection, which finishes, disconnects the power supply of spectrometer 1, light source 2 and micro syringe pump 3；

Clean cavity 4-1-1:

Using finishing, cavity 4-1-1 is cleaned, clean distilled water is mixed to the note of some detergent injection micro syringe pumps 7 It penetrates in pipe, micro syringe pump 7 is injected the distilled water for mixing detergent in cavity 4-1-1 by a channel by output end, micro- The distilled water output end of syringe pump 7 constantly pressurizes cavity 4-1-1 filling completely, and then the distilled water in cavity 4-1-1 is in pressure Under the action of flow into waste liquid pool 6 in, cleaning a period of time can by the dirty agent of cavity 4-1-1 dissolve clean, after cleaning up It injects some distilled water to be rinsed, the distilled water not being discharged finally is by the piston 4-1-2 of the bottom cavity 4-1-1 apocenosis passage The distilled water for unpluging, therefore not being discharged is flowed away by the apocenosis passage of the bottom cavity 4-1-1, and passes through drain by hair dryer Channel is dried to cavity 4-1-1 blowing, guarantees the drying and cleaning of cavity 4-1-1.

Claims (9)

1. corn fruit biological information detection device, including microscope (4), it is characterised in that: the objective table (4- of microscope (4) 1) it is provided with cavity (4-1-1) in, cavity (4-1-1) two relative side is respectively equipped with a channel, and the channel can be by cavity It is connected to outside (4-1-1) and objective table (4-1), liquid to be detected is filled in cavity (4-1-1), liquid to be detected is corn fruit Biology extracts detection liquid,

Corn fruit biological information detection device further includes spectrometer (1), light source (2), detection fiber (3) and gravity sensor (4-2)；

Detection fiber (3) is the optical fiber that can be realized SPR phenomenon, and sensing groove closed at both ends is provided in detection fiber (3), is passed Sense bottom portion of groove is fibre core (3-1), and the surface fibre core (3-1) is coated with nanometer metallic film (3-2), and nanometer metallic film (3-2) is The metallic film of SPR phenomenon can be excited, the sensing groove of detection fiber (3) is located in cavity (4-1-1), and guarantees to sense recessed Slot is completely immersed in liquid to be detected, and one end of detection fiber (3) extend out to objective table (4-1) outside, detection light from a channel The other end of fine (3) extend out to objective table (4-1) outside from another channel,

The emergent light of light source (2) is incident to one end of detection fiber (3), and the light of detection fiber (3) other end outgoing is incident to light Spectrometer (1),

Gravity sensor (4-2) is used to detect the measured object weight on the surface objective table (4-1),

The camera lens of microscope (4) is electron microscopic camera lens (5).

2. corn fruit biological information detection device according to claim 1, it is characterised in that: the cavity (4-1- 1) bottom is provided with apocenosis passage, the lower space connection of apocenosis passage and objective table (4-1), and apocenosis passage is equipped with piston (4-1- 2), piston (4-1-2) is for blocking apocenosis passage.

3. corn fruit biological information detection device according to claim 1 or 2, it is characterised in that: the device further includes Micro syringe pump (7) and waste liquid pool (6), by prepare liquid injection cavity (4-1-1), extra prepare liquid flows into micro syringe pump (7) Waste liquid pool (6).

4. corn fruit biological information detection device according to claim 3, it is characterised in that: the corn fruit is raw Object information detector further includes computer, the image signal output end of electron microscopic camera lens (5) and the picture signal of computer Input terminal connection.

5. corn fruit biological information detection device according to claim 1,2 or 4, it is characterised in that: the light source (2) emergent light is incident on the light incident side of detection fiber (3) by single-mode tail fiber (2-1), and the emergent light of detection fiber (3) is logical Cross the optical receiving end that multimode fibre (1-1) is incident on spectrometer (1).

6. corn fruit biological information detection device according to claim 5, it is characterised in that: the detection fiber It (3) is step optical fiber, gradual change multimode fibre or single mode optical fiber.

7. corn fruit biological information detection device according to claim 5, it is characterised in that: the nano metal is thin Film (3-2) is golden film or silverskin.

8. corn fruit biological information detection device according to claim 6 or 7, it is characterised in that: the light source (2) The light of outgoing is the white light of 450nm-1100nm wave band.

9. corn fruit biological information detection device according to claim 8, it is characterised in that: the cavity (4-1- It 1) is cuboid cavity.