CN103705545B - A kind of aweto micropowder preparation and preparation method thereof - Google Patents
A kind of aweto micropowder preparation and preparation method thereof Download PDFInfo
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- CN103705545B CN103705545B CN201310714886.1A CN201310714886A CN103705545B CN 103705545 B CN103705545 B CN 103705545B CN 201310714886 A CN201310714886 A CN 201310714886A CN 103705545 B CN103705545 B CN 103705545B
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Abstract
The present invention relates to a kind of aweto micropowder preparation, individually crushed by the polypide to cordyceps sinensis and stroma, and be controlled and adjust in the technological parameter to crushing process, it has been finally made process stabilizing, the aweto micropowder preparation that product quality is obviously improved.Of the invention farthest reduce is crushed and destruction of the freezing dry process to product quality, and the artificial industrialization to cordyceps sinensis brings good economic benefit.
Description
Technical field
Active ingredient enrichment can be significantly improved the present invention relates to cordyceps sinensis preparation and preparation method thereof, especially one kind
Aweto micropowder preparation of degree and preparation method thereof.
Background technology
Cordyceps sinensis is also known as cordyceps sinensis, is the traditional rare Chinese medicine of China, with cough-relieving, resolving sputum, reduction serum cholesterol, regulation
With the multiple pharmacological effect such as strengthen immunity, antitumor.In recent years, cordyceps sinensis demand is increased severely both at home and abroad.But due to winter worm
Summer grass has its strict parasitics and special growth geographical environment, and yield is fairly limited, and medicine source is more in short supply.Plus in recent years
Cordyceps sinensis price is high, and yield is reduced so that take which kind of mode effectively to turn into one tightly using existing Cordyceps Resources
Urgent problem.
Use of the traditional approach to cordyceps sinensis, takes decoction more, steeps in wine, the side such as health care buccal tablets is prepared into other medicinal materials
Formula, but cordyceps sinensis passes through high-temperature process, including protein and other active ingredients by thermally labile are corrupted such that the winter
Worm summer grass takes conventional method to process in use, effective rate of utilization is very low, and it is high to form price, low-quality vicious circle.
The stroma of cordyceps sinensis comes from the head of host larva, is found by our the abundant researchs to cordyceps sinensis, the winter
The polypide of worm summer grass and stroma are widely different in terms of cell composition and structure, fiber content, active principle content.Polypide portion
Divide and also have other things such as polypide enteron aisle in the nutriment for containing in epidermis and being enriched with the compositions, and polypide such as heavy metal and miscellaneous bacteria
Matter；And stroma does not have heavy metal, miscellaneous bacteria and other impurity, wherein being nutriment.If stroma is crushed jointly with polypide
Removal of impurities again, can not only influence the quality of cordyceps sinensis, and reduce the active ingredient enrichment of stroma, reduce the battalion of product
Value is supported, causes the loss of a part of active ingredient.
On this basis, in order to which more fully using precious Cordyceps Resources, we providing one kind can show
Write aweto micropowder preparation of raising therapeutic effect and preparation method thereof.
Patent application CN200710048651 disclose cordyceps sinensis breaking method and with crush after cordyceps sinensis system
Standby preparation, belongs to medicine and field of food.Specifically using the method for crushing polypide and stroma respectively.By worm
Body is crushed respectively with stroma, can be according to polypide and the respective cell composition of stroma and structure, fiber content, active principle content
Suitable pulverization conditions are set, loss of the long-time high temperature crushing to polypide part active ingredient is reduced, meets the public to winter worm
The demand of summer grass preparation.But this is only preliminary idea, the quality high winter can not be obtained only by simply crushing respectively
Worm summer grass product, in addition it is also necessary to investigated on the factor that product quality is influenceed in crushing process, preparation can significantly improve treatment
The cordyceps sinensis product of effect.
Patent application CN200920301520 discloses Cordyceps sinensis tablet, and it is the piece suppressed by primary raw material of cordyceps sinensis
Agent.Drug ingedient is homogeneous between the technical problem solved is to provide a kind of each, Cordyceps sinensis tablet without flower piece, is by least two layers
The tablet of lamella composition；Wherein, at least one layer of lamella is to be prepared after cordyceps sinensis stroma is crushed, and at least one layer of lamella is with cordyceps sinensis
Polypide is prepared after crushing.Above lower leaf between lamella, or with inside and outside layering.By above-mentioned improvement, when each lamella is used
When different material prepares tablet, by control the weight or thickness parameter of each lamella may be such that in gained tablet contained component and
The content of component is consistent, and control errors are in allowed band value.The technical scheme reduces blend step, when having saved operation
Between, technique is easy, can be achieved, without increasing equipment cost, is provided to prepare Cordyceps sinensis tablet in original tablet manufacturing equipment
A kind of new selection.Although that application discloses the tablet that cordyceps sinensis polypide and stroma are prepared respectively, by polypide and stroma system
It is standby not improve the quality of cordyceps sinensis inherently into multilayer tablet.
Patent application CN201210294303 discloses the low-temperature grinding method of cordyceps sinensis and its cordyceps sinensis of extension
Micro mist and ultrafine preparation, aweto micropowder and Ultramicro-powder after crushing can be used for making solid, liquid, semifluid,
The related preparations such as freeze-dried powder, the present invention relates to food, health products and medical scope, it is characterized in that being not higher than 0 DEG C of condition in temperature
Under, natural cordyceps are carried out without integrally being crushed in the case of any auxiliary material, the aweto micropowder and Ultramicro-powder of acquisition
Original active ingredient can be retained to greatest extent, the popular consumption demand to cordyceps sinensis is met.This application is to cordyceps sinensis
Overall crushing is carried out, still it cannot be guaranteed that product quality is significantly improved.
The content of the invention
To overcome the deficiencies in the prior art, the present inventor is had found by lot of experiments, by right
The polypide of cordyceps sinensis and stroma are individually crushed, and are controlled and are adjusted in the technological parameter to crushing process, finally
Process stabilizing has been made, the aweto micropowder preparation that product quality is obviously improved.
The present invention is realized by the following method：
A kind of aweto micropowder preparation, it is characterised in that be prepared from by following steps：
1) sorting cordyceps sinensis polypide and stroma, polypide remove epidermis, and after polypide and stroma cleaning, granularity is crushed to respectively
For the coarse powder of 40-80 mesh, polypide coarse powder and stroma coarse powder are respectively obtained；
2) pure water is added into polypide coarse powder and stroma coarse powder；Respectively obtain polypide coarse powder liquid and stroma coarse powder liquid；
3) use the oscillatory ball mill using zirconium ball as impact bead to carry out nanometer pulverization 2~4 hours under normal temperature, obtain granularity
Polypide micro mist liquid and stroma micro mist liquid for 400-800nm；
4) polypide micro mist liquid and stroma micro mist liquid are subjected to vacuum freeze drying, the freezing dry process is pre-freezing temperature
For -40 DEG C, 1.5~2.5h is maintained；The temperature in primary drying stage is -30 DEG C~-35 DEG C, maintains 7.5~8.5h, absolute pressure
For 4~7Pa；16~20 DEG C are warming up to 0.5 DEG C/min speed, into the redrying stage, 1.5~2.5h is maintained, definitely
Pressure is 16~25Pa；
5) using step 4) polypide powder obtained by freeze-drying and stroma powder as active component be prepared into pharmacy respectively
Upper acceptable preparation.
Preferably, the step 2) in the addition of pure water be 1~2 times of coarse powder；
Preferably, the step 4) in freeze-drying method, the pre-freeze process is 2.0h.
Preferably, the step 4) in freeze-drying method, the temperature in the primary drying stage is -32 DEG C, is maintained
8.0h, absolute pressure is 6Pa.
Preferably, the step 4) in freeze-drying method, the temperature in the redrying stage is 18 DEG C, is maintained
2.0h, absolute pressure is 18Pa.
The advantage of the invention is that：
1) stroma is crushed respectively with polypide, the product quality of cordyceps sinensis has been effectively ensured, and be easily removed heavy metal,
The impurity such as miscellaneous bacteria, improve product yield.
2) by fine powder after the first coarse powder of stroma and polypide, it is to avoid direct fine powder causes grinding time not enough, may not reach
The granularity of crushing, the performance of impact effect.
3) coarse powder is subjected to ultra micro nanometer pulverization under saturation state, when can not only effectively avoid the cordyceps sinensis from crushing due to
Protein isoreactivity composition is destroyed caused by temperature rise, the problem of Quality Down, and can effectively reach 400-800nm
Optimal granularity so that the active ingredient of cordyceps sinensis is more easy to be absorbed in human body, and bioavilability is significantly improved.
4) by the control to freeze drying process condition, freezing dry process is farthest reduced to product quality
Destruction, the artificial industrialization to cordyceps sinensis brings good economic benefit.
Embodiment
Embodiment 1
A kind of aweto micropowder preparation, it is characterised in that be prepared from by following steps：
1) sorting cordyceps sinensis polypide and stroma, polypide remove epidermis, and after polypide and stroma cleaning, granularity is crushed to respectively
For the coarse powder of 40-80 mesh, polypide coarse powder and stroma coarse powder are respectively obtained；
2) 1.5 times of amount pure water are added into polypide coarse powder and stroma coarse powder；Respectively obtain polypide coarse powder liquid and stroma is thick
Powder liquid；
3) oscillatory ball mill using zirconium ball as impact bead is used to carry out nanometer pulverization 2 hours under normal temperature, obtaining granularity is
400-800nm polypide micro mist liquid and stroma micro mist liquid；
4) polypide micro mist liquid and stroma micro mist liquid are subjected to vacuum freeze drying, the freezing dry process is pre-freezing temperature
For -40 DEG C, 2.5h is maintained；The temperature in primary drying stage is -30 DEG C, maintains 7.5h, absolute pressure is 4Pa；With 0.5 DEG C/min
Speed be warming up to 20 DEG C, into the redrying stage, maintain 2.5h, absolute pressure is 25Pa；
5) using step 4) freeze-drying obtained by polypide powder and stroma powder as active component respectively with appropriate amount of starch
Mix, fully mixed after sieving, be distributed into capsule and produce, every 0.2g or every 0.4g.
Embodiment 2
1) sorting cordyceps sinensis polypide and stroma, polypide remove epidermis, and after polypide and stroma cleaning, granularity is crushed to respectively
For the coarse powder of 40-80 mesh, polypide coarse powder and stroma coarse powder are respectively obtained；
2) 1 times of amount pure water is added into polypide coarse powder and stroma coarse powder；Respectively obtain polypide coarse powder liquid and stroma coarse powder
Liquid；
3) oscillatory ball mill using zirconium ball as impact bead is used to carry out nanometer pulverization 4 hours under normal temperature, obtaining granularity is
400-800nm polypide micro mist liquid and stroma micro mist liquid；
4) polypide micro mist liquid and stroma micro mist liquid are subjected to vacuum freeze drying, the freezing dry process is pre-freezing temperature
For -40 DEG C, 2.5h is maintained；The temperature in primary drying stage is -30 DEG C, maintains 7.5h, absolute pressure is 5Pa；With 0.5 DEG C/min
Speed be warming up to 20 DEG C, into the redrying stage, maintain 1.5h, absolute pressure is 16Pa；
5) using step 4) freeze-drying obtained by polypide powder and stroma powder as active component respectively with appropriate amount of starch
Mix, fully mixed after sieving, be distributed into capsule and produce, every 0.2g or every 0.4g.
Embodiment 3
A kind of aweto micropowder preparation, it is characterised in that be prepared from by following steps：
1) sorting cordyceps sinensis polypide and stroma, polypide remove epidermis, and after polypide and stroma cleaning, granularity is crushed to respectively
For the coarse powder of 40-80 mesh, polypide coarse powder and stroma coarse powder are respectively obtained；
2) 2 times of amount pure water are added into polypide coarse powder and stroma coarse powder；Respectively obtain polypide coarse powder liquid and stroma coarse powder
Liquid；
3) oscillatory ball mill using zirconium ball as impact bead is used to carry out nanometer pulverization 3 hours under normal temperature, obtaining granularity is
400-800nm polypide micro mist liquid and stroma micro mist liquid；
4) polypide micro mist liquid and stroma micro mist liquid are subjected to vacuum freeze drying, the freezing dry process is pre-freezing temperature
For -40 DEG C, 2.0h is maintained；The temperature in primary drying stage is -32 DEG C, maintains 8.0h, absolute pressure is 6Pa；With 0.5 DEG C/min
Speed be warming up to 18 DEG C, into the redrying stage, maintain 2.0h, absolute pressure is 18Pa；
5) using step 4) freeze-drying obtained by polypide powder and stroma powder as active component respectively with appropriate amount of starch
Mix, fully mixed after sieving, be distributed into capsule and produce, every 0.2g or every 0.4g.
Reference examples 1
Polypide and stroma are not sorted, cordyceps sinensis is integrally prepared into ultra-micro powder according to the method for embodiment 3.
Reference examples 2
Method according to embodiment 3 is prepared into ultra-micro powder, and wherein parameters of freeze-drying process is replaced with：Pre-freezing temperature is -40
DEG C, maintain 2.0h；The temperature in primary drying stage is -20 DEG C, maintains 8.0h, absolute pressure is 6Pa；With 0.5 DEG C/min speed
Degree is warming up to 25 DEG C, into the redrying stage, maintains 2.0h, absolute pressure is 18Pa.
Reference examples 3
According to the solid fermentation bacterium of aweto made from the condition of embodiment 1 in patent application CN201310124056
Thread, its freeze-drying process is：By the cordyceps mycelia after concentration with -0.6 DEG C/min of cooling rate snap frozen to -30 DEG C, dimension
Hold after 2 hours, be put into hothouse and be dried.Heating plate is warming up to after 45 DEG C, is kept for 9 hours, then is cooled to 35 DEG C, holding 5
Hour, overall process keeps maximum vacuum.Products therefrom moisture≤5%.
The testing result of the active ingredient of test example 1
According to following index, to control group 1-3, embodiment 1-3, (control group 2 and embodiment group 1-3 determine stroma part
Powder content) product compareed, wherein total amino acid, mannitol, adenosine content be Chinese caterpillar fungus bacterial filament dry product in weight
Percentage composition is measured, concrete outcome see the table below (average that each numerical value is 10 batches of samples)：
| Index | Standards of pharmacopoeia | Control group 1 | Control group 2 | Control group 3 | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Total amino acid content (%) | ≥30 | 32.52 | 38.53 | 30.84 | 48.51 | 49.82 | 53.45 |
| Mannitol content (%) | ≥7.0 | 7.26 | 8.51 | 6.49 | 11.52 | 12.32 | 12.84 |
| Adenosine content (%) | ≥0.08 | 0.074 | 0.193 | 0.088 | 0.261 | 0.284 | 0.326 |
It can be drawn by above-mentioned test example, the aweto micropowder preparation prepared by the present invention can be reduced farthest
Crush and destruction of the freezing dry process to product quality, be able to significantly carry so as to obtain a kind of process stabilizing, product quality
The aweto micropowder preparation risen, the artificial industrialization to cordyceps sinensis brings good economic benefit.
Finally it should be noted that the foregoing is only the preferred embodiments of the present invention, it is not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic.
Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., should be included in the present invention's
Within protection domain.
Claims (5)
1. a kind of preparation method of aweto micropowder preparation, it is characterised in that be prepared from by following steps：
1) sorting cordyceps sinensis polypide and stroma, polypide remove epidermis, and after polypide and stroma cleaning, granularity is crushed to respectively is
The coarse powder of 40-80 mesh, respectively obtains polypide coarse powder and stroma coarse powder；
2) pure water is added into polypide coarse powder and stroma coarse powder；Respectively obtain polypide coarse powder liquid and stroma coarse powder liquid；
3) oscillatory ball mill using zirconium ball as impact bead is used to carry out nanometer pulverization 2~4 hours under normal temperature, obtaining granularity is
400~800nm polypide micro mist liquid and stroma micro mist liquid；
4) polypide micro mist liquid and stroma micro mist liquid are subjected to vacuum freeze drying, the freezing dry process be pre-freezing temperature for-
40 DEG C, maintain 1.5~2.5h；The temperature in primary drying stage is -30 DEG C~-35 DEG C, maintains 7.5~8.5h, absolute pressure is 4
~7Pa；16~20 DEG C are warming up to 0.5 DEG C/min speed, into the redrying stage, 1.5~2.5h, absolute pressure is maintained
For 16~25Pa；
5) using step 4) freeze-drying obtained by polypide powder and stroma powder be prepared into and pharmaceutically may be used respectively as active component
The preparation of receiving.
2. preparation method as claimed in claim 1, wherein the step 2) in the addition of pure water be 1-2 times of coarse powder.
3. preparation method as claimed in claim 1, wherein the step 4) in freeze-drying method, the pre-freeze process is
2.0h。
4. preparation method as claimed in claim 1, wherein the step 4) in freeze-drying method, the primary drying stage
Temperature be -32 DEG C, maintain 8.0h, absolute pressure is 6Pa.
5. preparation method as claimed in claim 1, wherein the step 4) in freeze-drying method, the redrying stage
Temperature be 18 DEG C, maintain 2.0h, absolute pressure is 18Pa.
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| CN105737528A (en) * | 2014-12-08 | 2016-07-06 | 王元龙 | Cordyceps sinensis vacuum freeze-drying processing method |
| CN113827623A (en) * | 2020-09-17 | 2021-12-24 | 东莞市东阳光冬虫夏草研发有限公司 | Cordyceps sinensis wall-breaking preparation and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019898A (en) * | 2007-03-16 | 2007-08-22 | 张雪峰 | Crushing method and prepn of aweto |
| CN102631377A (en) * | 2012-04-28 | 2012-08-15 | 三普药业股份有限公司 | Cordyceps sinensis freeze-dried nano powder tablet and preparation method thereof |
| CN102784174A (en) * | 2012-08-19 | 2012-11-21 | 陈玉龙 | Process for preparing Chinese caterpillar fungus micropowder and preparation thereof |
-
2013
- 2013-12-13 CN CN201310714886.1A patent/CN103705545B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019898A (en) * | 2007-03-16 | 2007-08-22 | 张雪峰 | Crushing method and prepn of aweto |
| CN102631377A (en) * | 2012-04-28 | 2012-08-15 | 三普药业股份有限公司 | Cordyceps sinensis freeze-dried nano powder tablet and preparation method thereof |
| CN102784174A (en) * | 2012-08-19 | 2012-11-21 | 陈玉龙 | Process for preparing Chinese caterpillar fungus micropowder and preparation thereof |
Non-Patent Citations (1)
| Title |
|---|
| 冬虫夏草真空冷冻干燥的实验研究;徐成海，等;《真空》;19941231(第06期);34－37 * |
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