CN103651124B - 一种生姜再生植株的诱导方法 - Google Patents
一种生姜再生植株的诱导方法 Download PDFInfo
- Publication number
- CN103651124B CN103651124B CN201310591420.7A CN201310591420A CN103651124B CN 103651124 B CN103651124 B CN 103651124B CN 201310591420 A CN201310591420 A CN 201310591420A CN 103651124 B CN103651124 B CN 103651124B
- Authority
- CN
- China
- Prior art keywords
- bud
- rhizoma zingiberis
- zingiberis recens
- culture
- root
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000196324 Embryophyta Species 0.000 title claims abstract description 12
- 238000013459 approach Methods 0.000 title claims abstract description 12
- 230000008929 regeneration Effects 0.000 title claims abstract description 10
- 238000011069 regeneration method Methods 0.000 title claims abstract description 10
- 244000273928 Zingiber officinale Species 0.000 title claims abstract description 9
- 235000006886 Zingiber officinale Nutrition 0.000 title claims abstract description 9
- 235000008397 ginger Nutrition 0.000 title claims abstract description 9
- 239000001841 zingiber officinale Substances 0.000 title claims abstract description 9
- 238000011081 inoculation Methods 0.000 claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 6
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229920000153 Povidone-iodine Polymers 0.000 claims abstract description 4
- 229960001621 povidone-iodine Drugs 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 229920001817 Agar Polymers 0.000 claims description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 13
- 229930006000 Sucrose Natural products 0.000 claims description 13
- 239000008272 agar Substances 0.000 claims description 13
- 239000005720 sucrose Substances 0.000 claims description 13
- 230000015572 biosynthetic process Effects 0.000 claims description 11
- 239000012153 distilled water Substances 0.000 claims description 9
- 230000001939 inductive effect Effects 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000002689 soil Substances 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 235000013372 meat Nutrition 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- 239000012879 subculture medium Substances 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 4
- 244000052616 bacterial pathogen Species 0.000 abstract description 2
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 239000005708 Sodium hypochlorite Substances 0.000 abstract 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 abstract 1
- 239000007943 implant Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012090 tissue culture technique Methods 0.000 description 2
- 241000234299 Zingiberaceae Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310591420.7A CN103651124B (zh) | 2013-11-22 | 2013-11-22 | 一种生姜再生植株的诱导方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310591420.7A CN103651124B (zh) | 2013-11-22 | 2013-11-22 | 一种生姜再生植株的诱导方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103651124A CN103651124A (zh) | 2014-03-26 |
CN103651124B true CN103651124B (zh) | 2016-07-20 |
Family
ID=50290844
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310591420.7A Expired - Fee Related CN103651124B (zh) | 2013-11-22 | 2013-11-22 | 一种生姜再生植株的诱导方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103651124B (zh) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104585027B (zh) * | 2014-12-24 | 2016-06-29 | 广西南宁智翠科技咨询有限公司 | 一种生姜组培苗的炼苗方法 |
CN105794637A (zh) * | 2014-12-31 | 2016-07-27 | 张琴芳 | 一种生姜无毒快速育苗方法 |
CN108812315A (zh) * | 2018-06-27 | 2018-11-16 | 芜湖东源新农村开发股份有限公司 | 生姜的组织培养方法 |
CN111771725A (zh) * | 2020-07-31 | 2020-10-16 | 潍坊兴旺生物种业有限公司 | 一种优化的生姜脱毒苗再生扩繁方法及其产业化流程的开发 |
CN115589945A (zh) * | 2022-10-10 | 2023-01-13 | 长江大学(Cn) | 一种开放式培养生姜试管苗的方法 |
CN115769786B (zh) * | 2022-11-30 | 2023-12-19 | 安徽农业大学 | 以交替式组织培养的方式获取铜陵白姜再生苗的方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR0160086B1 (ko) * | 1995-06-21 | 1998-11-16 | 김광희 | 종강용 생강 인공종묘의 제조방법 |
CN1596594A (zh) * | 2004-09-22 | 2005-03-23 | 孙玉珂 | 生姜浸种直播剂 |
CN1930951A (zh) * | 2006-09-29 | 2007-03-21 | 徐坤 | 一种生姜脱毒苗的培育方法 |
CN101147466A (zh) * | 2006-09-22 | 2008-03-26 | 中国农业科学院蔬菜花卉研究所 | 一种微型姜苗组培快繁培养基及组培快繁方法 |
-
2013
- 2013-11-22 CN CN201310591420.7A patent/CN103651124B/zh not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR0160086B1 (ko) * | 1995-06-21 | 1998-11-16 | 김광희 | 종강용 생강 인공종묘의 제조방법 |
CN1596594A (zh) * | 2004-09-22 | 2005-03-23 | 孙玉珂 | 生姜浸种直播剂 |
CN101147466A (zh) * | 2006-09-22 | 2008-03-26 | 中国农业科学院蔬菜花卉研究所 | 一种微型姜苗组培快繁培养基及组培快繁方法 |
CN1930951A (zh) * | 2006-09-29 | 2007-03-21 | 徐坤 | 一种生姜脱毒苗的培育方法 |
Non-Patent Citations (2)
Title |
---|
生姜组培苗工厂化生产技术;潘菊等;《湖南农机》;20120731;第39卷(第7期);第231页,尤其是第1-3节 * |
生姜茎尖的组织培养及试管苗快繁体系的研究;李红梅等;《山东科学》;20081031;第21卷(第5期);第36-38页 * |
Also Published As
Publication number | Publication date |
---|---|
CN103651124A (zh) | 2014-03-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103651124B (zh) | 一种生姜再生植株的诱导方法 | |
CN103120126B (zh) | 利用间歇浸没生物反应器进行金线莲组织培养扩繁方法 | |
CN105145359B (zh) | 一种羊齿天冬组织培养快速繁殖方法 | |
CN103283595B (zh) | 一种草莓茎尖组织初代培养方法 | |
CN102845308B (zh) | 一种铁皮石斛组织培养一步成苗的方法 | |
CN104770300B (zh) | 一种快速繁育绵毛鹿茸草幼苗的方法 | |
CN104396742B (zh) | 用五步法诱导淡黄花百合珠芽愈伤组织再分化出无菌苗的方法 | |
CN103734014A (zh) | 一种地枫皮的组织培养快速繁殖方法 | |
CN104472355A (zh) | 一种箭叶淫羊藿越冬芽快速繁殖方法 | |
CN103270946B (zh) | 一种铁皮石斛种苗生产与炼苗同步方法 | |
CN106106187A (zh) | 一种建立泰山黄精高频再生体系的方法及培养基 | |
CN105409771A (zh) | 一种佛手参的快速繁殖方法 | |
CN101926284B (zh) | 一种川附子组织培养及快速繁殖方法 | |
CN107484666A (zh) | 一种白芍的组织培养快繁方法 | |
CN105191803B (zh) | 一种铁皮石斛组培袋苗生产方法 | |
CN104719168B (zh) | 利用间歇浸没生物反应器培育白芨种苗的方法 | |
CN106718875B (zh) | 石斛育苗方法 | |
CN104813931A (zh) | 一种铁皮石斛组培快繁的方法 | |
CN104813932A (zh) | 一种铁皮石斛的离体保存方法 | |
CN106416972A (zh) | 一种快速培养白芨种苗的方法 | |
CN105918132A (zh) | 一种海州常山快速繁育方法 | |
CN110024688A (zh) | 一种锦鸡儿芽增殖与植株再生的方法及其培养基 | |
CN105075860A (zh) | 一种灯盏花组培育苗方法 | |
CN104396746A (zh) | 一种黄花贝母不定芽诱导增殖方法 | |
CN107873524A (zh) | 一种珠芽蓼茎段组培快繁方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
CB03 | Change of inventor or designer information |
Inventor after: Su Jianli Inventor before: Zhang Ming |
|
COR | Change of bibliographic data | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20160606 Address after: 266061 Shandong, Laoshan, Hongkong East Road, No. Pioneering Park, biological park, room 248, No. 239 Applicant after: QINGDAO WINCHANCE TECHNOLOGY Co.,Ltd. Address before: 266071 Shandong city of Qingdao province Hongkong City Road No. 132, building 3, 202 households Applicant before: Zhang Ming |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20181130 Address after: Room 1402, Ziyang Building, 99 Mingzhu Road, Huaishang District, Bengbu City, Anhui Province Patentee after: Bengbu HRABERO Intellectual Property Service Co.,Ltd. Address before: 266061 Qingdao Hongkong Laoshan road 248, Pioneer Park, 239 Biological Park 239 Patentee before: QINGDAO WINCHANCE TECHNOLOGY Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20181212 Address after: 409009 Baijiahe Road, Zhengyang Industrial Park, Qianjiang District, Chongqing Patentee after: CHONGQING QIANJIANG APENG TECHNOLOGY ENTERPRISE INCUBATOR CO.,LTD. Address before: Room 1402, Ziyang Building, 99 Mingzhu Road, Huaishang District, Bengbu City, Anhui Province Patentee before: Bengbu HRABERO Intellectual Property Service Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160720 Termination date: 20211122 |
|
CF01 | Termination of patent right due to non-payment of annual fee |