CN103650816B - A kind of method that sweet osmanthus micro cuttage is numerous soon - Google Patents
A kind of method that sweet osmanthus micro cuttage is numerous soon Download PDFInfo
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- CN103650816B CN103650816B CN201210355138.4A CN201210355138A CN103650816B CN 103650816 B CN103650816 B CN 103650816B CN 201210355138 A CN201210355138 A CN 201210355138A CN 103650816 B CN103650816 B CN 103650816B
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- cave
- cuttage
- matrix
- cutting
- sweet osmanthus
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- 244000242564 Osmanthus fragrans Species 0.000 title claims abstract description 18
- 235000019083 Osmanthus fragrans Nutrition 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000005520 cutting process Methods 0.000 claims abstract description 26
- 239000011159 matrix material Substances 0.000 claims abstract description 19
- 238000007789 sealing Methods 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 239000002689 soil Substances 0.000 claims abstract description 4
- 240000007594 Oryza sativa Species 0.000 claims abstract description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 3
- 238000000855 fermentation Methods 0.000 claims abstract description 3
- 230000004151 fermentation Effects 0.000 claims abstract description 3
- 239000010903 husk Substances 0.000 claims abstract description 3
- 235000019362 perlite Nutrition 0.000 claims abstract description 3
- 239000010451 perlite Substances 0.000 claims abstract description 3
- 235000009566 rice Nutrition 0.000 claims abstract description 3
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 6
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 6
- 239000006013 carbendazim Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 230000009286 beneficial effect Effects 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000005556 hormone Substances 0.000 claims description 3
- 229940088597 hormone Drugs 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims description 3
- 240000007643 Phytolacca americana Species 0.000 claims description 2
- 239000003337 fertilizer Substances 0.000 claims description 2
- 239000003292 glue Substances 0.000 claims description 2
- 208000015181 infectious disease Diseases 0.000 claims description 2
- 230000000422 nocturnal effect Effects 0.000 claims description 2
- 230000000050 nutritive effect Effects 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- 229920003023 plastic Polymers 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims 2
- 229930192334 Auxin Natural products 0.000 claims 1
- 239000002363 auxin Substances 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 235000019994 cava Nutrition 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 5
- 102000018997 Growth Hormone Human genes 0.000 abstract description 3
- 108010051696 Growth Hormone Proteins 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 238000004904 shortening Methods 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract 1
- 230000003020 moisturizing effect Effects 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 235000019082 Osmanthus Nutrition 0.000 description 1
- 241000333181 Osmanthus Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Landscapes
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to agricultural technology field, be specifically related to a kind of method that sweet osmanthus micro cuttage is numerous soon, select the cave dish of 40*40*45mm as matrix container, and on the dish of cave, build the support of overlay film; Add 10% ash with 30% rural area soil to add 20% perlite and add 40% and sterilize as matrix through the rice husk mixing of fermentation; Select raw lignified sweet osmanthus branch then as treating cuttage bar, to be cut into segment according to interval, every section only containing bud joint, a two panels leaf; Cleaning and sterilizing is carried out to cutting and section wound in cutting is inserted cave dish after carrying out sealing; Somatotropin is dripped to each slotting cave; To cave dish sealing and moisturizing.Sweet osmanthus cave of the present invention dish micro cuttage fast breeding technique, by shortening the length of cuttage branch, considerably increases the numerous multiple of expansion of PROPAGATION BY TRANSPLANTING LAUREI CUTTING; By improvement plug media, pretreatment before cuttage, make its survival rate to more than 95%, by control temperature and humidity and use somatotropin process, make its rooting rate can reach more than 80% in one month.
Description
Technical field
The invention belongs to agricultural technology field, be specifically related to a kind of method that sweet osmanthus micro cuttage is numerous soon.
Background technology
The normal cutting propagation of sweet osmanthus is because of the restriction by factors such as time in season, area surroundings difference and sweet osmanthus growth rate, exist take root slow, growth is slow, it is long and expand the defects such as numerous multiple is low to expand numerous cycle, especially for the improved seeds of some preciousnesses, cause delaying to promote owing to originating rare.The present invention by with cave dish as independently seedbed, make sweet osmanthus cuttage break away from the restriction of soil condition, nursery can be cultivated whenever and wherever possible, simultaneously due to cave dish each slotting cave between relative isolation, also greatly limit the propagation of damage by disease and insect; By shortening cutting length, reduce joint number contained by cutting, thus considerably increase cuttage and expand numerous breeding rate, it expands more than 5 times that numerous multiple is normal cutting propagation, and the popularization for the rare precious osmanthus cultivars in source has great using value.
Summary of the invention
The object of the present invention is to provide the numerous coefficient of a kind of expansion high, with low cost, simple and easy to do sweet osmanthus cuttage and quick-propagation technology.Use the method can greatly improve the breeding rate of sweet osmanthus cuttage, and greatly shorten the required time of taking root.
Advantage of the present invention is:
1, numerous multiple is expanded high.Owing to shortening cutting length, decrease joint number contained by cutting, thus considerably increase the availability of every root sweet osmanthus branch, it expands more than 5 times that numerous multiple is normal cutting propagation.
2, take root soon, survival rate is high.By soaking somatotropin, maintain higher humidity, cutting can up to 80% at about one month rooting rate, and one and a half months can reach 90%.Due to matrix mutually isolated in the dish of cave and the sterilization to matrix and cutting, the propagation of disease can greatly be reduced.Due to cave dish mutually closing to external world, also greatly reduce the threat of insect pest simultaneously.
3, raw material is easy to get, with low cost.Do not need a large amount of early investment and preparation, do not need complicated equipment support yet.Cave dish, matrix components, support and film these can purchase everywhere all on the market, price is also very cheap.
4, method is simple, is easy to management.Whole cuttage process is simple, not needing advanced technology and complicated operation, just can completing the whole process from preparing to seal within one day.Due to the independent sum sealing that whole cave is coiled, the later stage does not need too many management except squirting and adding water.
Accompanying drawing illustrates:
Fig. 1 is sealer cave dish schematic diagram.
Fig. 2 is the photo of taking root after cutting grows 30 days.
Fig. 3 is the photo after cutting grows 60 days.
In figure, 1 is film, and 2 is pole, and 3 is cave dish.
Specific embodiment:
(1) preparation of container: select the rigid plastics cave dish of 4*4*4.5cm as matrix container.For the ease of controlling temp. and humidity, whole cave dish is unsuitable excessive, at 1000 ~ 1500cm
2within.Cave dish surrounding fix 6 poles be convenient to cuttage after overlay film, pole is 10cm mono-section, and 5mm place, top is curved in 90 ° to prevent film to poke.
(2) preparation of matrix: with 30% rural area soil add 10% ash add 20% perlite add 40% through fermentation rice husk mixing as matrix, after fully mixing each component, matrix is inserted in Pan Cha cave, cave, matrix should not filled out overfill is beneficial to the isolation of each slotting cave matrix, thus prevents germ mutually to be infected by matrix.Finally irrigate with the carbendazim solution of 0.1%.
(3) pretreatment of cutting: selecting raw lignified sweet osmanthus branch then as treating cuttage bar, to rinse under branch being placed in after adopting back flowing water, then soaking 1 ~ 2 hour with the carbendazim solution of 0.1%.Every root branch is cut into the long segment of 1 ~ 2cm according to interval, upper end clip retains the distance of 2 ~ 3mm from axillalry bud, lower end clip at least retains the distance of 5mm from petiole, cuts to carry out glue sealing process to prevent the infection of germ to the wound of section in every section of cutting afterwards.Cutting lower end is soaked after 5 minutes and is taken out standby inserting in hormone solution.
(4) cuttage of cutting: the blade of all cuttings during cuttage keeps towards same direction as far as possible, vertically inserts Medium Culture and is about 5mm deeply by cutting, then press slightly tight around with hand.Insert the carbendazim solution spraying a time 0.1% with watering can on surface, with film, whole cave dish is covered sealing afterwards.
(5) management after inserting: slotting careful cave of sealing is coiled the place being placed in indoor non-direct sunlight or have in the booth of plastic film covering and cultivate.Daytime temperature should control within 25 ~ 30 DEG C, and nocturnal temperature is within 22 ~ 28 DEG C.Humidity is surveyed once for every two days, remains on about 85%.Treat that root grows to more than 3cm, just transfer to inside nutritive cube, suitably apply fertilizer and be beneficial to sprout.
Claims (4)
1. the method that sweet osmanthus micro cuttage is numerous soon, its main implementation step is: the preparation of (1) container: select 20x20mm capacity 40cc bottom bore 40mmx40mm, and the rigid plastics cave dish in 72 caves is as matrix container, and whole cave dish is at 1000 ~ 1500cm
2within, cave dish surrounding fix 6 poles be convenient to cuttage after overlay film, pole is 10cm mono-section, and 5mm place, top is curved in 90 ° to prevent film to poke; (2) preparation of matrix: with 30% rural area soil add 10% ash add 20% perlite add 40% through fermentation rice husk mixing as matrix, after fully mixing each component, matrix is inserted in Pan Cha cave, cave, matrix should not filled out overfill is beneficial to the isolation of each slotting cave matrix, thus prevent germ mutually to be infected by matrix, finally irrigate with the carbendazim solution of 0.1%; (3) pretreatment of cutting: select to give birth to lignified sweet osmanthus branch then as treating cuttage bar, rinse under branch being placed in after adopting back flowing water, then soak 1 ~ 2 hour with the carbendazim solution of 0.1%, every root branch is cut into the long segment of 1 ~ 2cm according to interval, upper end clip retains the distance of 2 ~ 3mm from axillalry bud, lower end clip at least retains the distance of 5mm from petiole, cut and carry out glue sealing process to prevent the infection of germ to the wound of section in every section of cutting afterwards, cutting lower end is soaked after 5 minutes and is taken out standby inserting in hormone solution; (4) cuttage of cutting: the blade of all cuttings during cuttage keeps towards same direction as far as possible, cutting vertically being inserted Medium Culture, to be about 5mm dark, then press slightly tight around with hand, insert the carbendazim solution spraying a time 0.1% with watering can on surface, with film, whole cave dish is covered sealing afterwards; (5) management after inserting: slotting careful cave of sealing is coiled in the place or green house being placed in indoor non-direct sunlight and cultivates, daytime temperature should control within 25 ~ 30 DEG C, nocturnal temperature is within 22 ~ 28 DEG C, humidity is surveyed once for every two days, remain on about 85%, treat that root grows to more than 3cm, just transfer to inside nutritive cube, suitably apply fertilizer and be beneficial to sprout.
2. the method that a kind of sweet osmanthus micro cuttage as claimed in claim 1 is numerous soon, is characterized in that: every section, the branch through blocking is only containing bud joint, a two panels leaf, and every sheet leaf only stays 2 ~ 3cm
2, redundance is cut.
3. the method that a kind of sweet osmanthus micro cuttage as claimed in claim 1 is numerous soon, is characterized in that: hormone used is auxin IAA (heteroauxin), and its concentration is 1.0mg/L.
4. the method that a kind of sweet osmanthus micro cuttage as claimed in claim 1 is numerous soon, is characterized in that: after sealing, in the dish of cave, air humidity remains on about 85%, within every two days, surveys once, and overdrying, then with watering can water spray, is crossed wet film of just taking off and ventilated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210355138.4A CN103650816B (en) | 2012-09-21 | 2012-09-21 | A kind of method that sweet osmanthus micro cuttage is numerous soon |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210355138.4A CN103650816B (en) | 2012-09-21 | 2012-09-21 | A kind of method that sweet osmanthus micro cuttage is numerous soon |
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| Publication Number | Publication Date |
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| CN103650816A CN103650816A (en) | 2014-03-26 |
| CN103650816B true CN103650816B (en) | 2016-04-06 |
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Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104025833A (en) * | 2014-04-28 | 2014-09-10 | 深圳文科园林股份有限公司 | Sweet osmanthus cuttage rapid propagation technology |
| CN104186188A (en) * | 2014-09-17 | 2014-12-10 | 重庆市涪陵区外湾花卉种植园 | Cultivation method for Osmanthus fragrans |
| CN104322250A (en) * | 2014-10-14 | 2015-02-04 | 深圳文科园林股份有限公司 | Efficient propagation method for maintaining chimera pineapple character stable inheritance |
| CN104355892A (en) * | 2014-11-19 | 2015-02-18 | 柳州市潮林机械有限公司 | Special nutritional soil for osmanthus tree and preparation method thereof |
| CN104920025B (en) * | 2015-06-04 | 2018-05-11 | 湖北天健农产品有限公司 | Cottage breeding method |
| CN105009919A (en) * | 2015-08-06 | 2015-11-04 | 张剑逸 | Osmanthus fragrans cutting seedling method |
| CN105638358A (en) * | 2016-01-09 | 2016-06-08 | 徐尤双 | Cultivation method of sweet-scented osmanthus seedlings |
| CN106973738A (en) * | 2016-01-15 | 2017-07-25 | 重庆晨之鑫生态农业发展有限公司 | The cuttage implantation methods of osmanthus fragrans |
| CN106386071A (en) * | 2016-08-31 | 2017-02-15 | 界首市金玖江农业发展有限公司 | Cutting and seedling growing method for sweet osmanthus |
| CN106416670A (en) * | 2016-08-31 | 2017-02-22 | 界首市金玖江农业发展有限公司 | Osmanthus fragrans planting method |
| CN106614725A (en) * | 2016-10-12 | 2017-05-10 | 许伟琦 | Growth regulating agent for osmanthus tree seedling culture, plantation and transplantation |
| CN107188700A (en) * | 2017-06-22 | 2017-09-22 | 和县赭洛山茶叶种植专业合作社 | A kind of cuttage and seedling culture method of osmanthus fragrans |
| CN108283189A (en) * | 2018-02-08 | 2018-07-17 | 金华市飞凌生物科技有限公司 | A kind of wave leaf gold osmanthus cuttage root-taking agent |
| CN108925261A (en) * | 2018-06-13 | 2018-12-04 | 山西省农业科学院生物技术研究中心 | A kind of black fruit fructus lycii micro cuttage rapid propagation method |
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| NL1011347C2 (en) * | 1999-02-19 | 2000-08-22 | B & B Bulbs & Flowers B V | Grow carrier. |
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| CN102523898A (en) * | 2012-03-02 | 2012-07-04 | 江苏美尚生态景观股份有限公司 | Seedling raising method for carrying out closed cuttage on twigs of osmanthus fragrans |
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Address after: Futian District Shennan Road Shenzhen city Guangdong province 518000 Shenzhen International Innovation Center A block 36 layer Applicant after: SHENZHEN WENKE LANDSCAPE Co.,Ltd. Address before: 518048 Guangdong city of Shenzhen province Futian District Binhe Central Valley 21 storey building Applicant before: SHENZHEN WENKE LANDSCAPE Co.,Ltd. |
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Application publication date: 20140326 Assignee: Guangdong Yaoda Financial Leasing Co.,Ltd. Assignor: SHENZHEN WENKE LANDSCAPE Co.,Ltd. Contract record no.: X2023980044229 Denomination of invention: A Method for Rapid Propagation of Osmanthus fragrans by Microcutting Granted publication date: 20160406 License type: Exclusive License Record date: 20231023 |
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Denomination of invention: A Method for Rapid Propagation of Osmanthus fragrans by Microcutting Effective date of registration: 20231024 Granted publication date: 20160406 Pledgee: Guangdong Yaoda Financial Leasing Co.,Ltd. Pledgor: SHENZHEN WENKE LANDSCAPE Co.,Ltd. Registration number: Y2023980062607 |