CN102257919A - Node cutting propagation technology for cotton - Google Patents

Node cutting propagation technology for cotton Download PDF

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Publication number
CN102257919A
CN102257919A CN 201110131903 CN201110131903A CN102257919A CN 102257919 A CN102257919 A CN 102257919A CN 201110131903 CN201110131903 CN 201110131903 CN 201110131903 A CN201110131903 A CN 201110131903A CN 102257919 A CN102257919 A CN 102257919A
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CN
China
Prior art keywords
cotton
soil
joint position
cuttage
branch
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CN 201110131903
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Chinese (zh)
Inventor
余政军
李瑞莲
周毅
贺鹏毅
余筱南
刘爱玉
刘少卿
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Hunan Agricultural University
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Hunan Agricultural University
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Priority to CN 201110131903 priority Critical patent/CN102257919A/en
Publication of CN102257919A publication Critical patent/CN102257919A/en
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Abstract

The invention relates to the technical field of asexual propagation of crops, in particular to a node cutting method for quickly propagating cotton seeds. The method comprises the following steps of: in a growing and developing season of cotton, cutting a main stem node or a fruit spur node into twigs which are 5 to 8cm long, and cutting the twigs in the way of one node and one leaf; arranging a seed bed in a leeward and sunward place, preparing a land finely to loosen soil, and sterilizing the soil, wherein moisture content takes a saturated state of surface soil layer as a reference; arching a film above cutting seedlings so as to cover the cutting seedlings and sealing the film on four sides so as to keep temperature and humidity; and transplanting the seedlings to a big land after 20 days, wherein cultivation and management of the big field are the same as the conventional cultivation and management. The transplanting survival rate of the cutting seedlings cultivated by the method is about 98 percent; and cost can be saved.

Description

Cotton joint position cottage propagation technology
Technical field
The invention belongs to crops vegetative propagation technique field, be specifically related to the grow cotton cuttage of joint position, the method for breeding seed fast.
Background technology
The breeding of cotton all is sexual propagation at present, promptly utilize seed to breed, if cotton joint position cottage propagation technology is applied in cotton scientific research, the production, the one, the proterties that can avoid occurring in the sexual propagation is separated, enlarge rapidly the high-quality population of cotton, for the utilization of merit provides good parent; The 2nd, can accelerate the breeding of special germ plasm resource, high-quality variant or wild cotton as finding often can not receive seed or seed seldom, adopt joint position cottage propagation this moment, then can increase the seed output fast; The 3rd, be used for hybrid vigour and fix, cottage propagation can keep all merits of parent; The 4th, can resume production rapidly, southern cotton growing area in China, after entering flood season,, will cause output of cotton to descend if cotton plants suffers waterlogging, even the meeting that has total crop failure, reseed this moment again, and season is slow, has a strong impact on cotton grower's income, if carry out the cotton cuttage after disaster-stricken, also can obtain higher output; The 5th, can promote the commercialization of cotton seedling, cuttage and seedling culture method is simple, easy to operate, seedling raise period short, can utilize production facility rapid cuttage in wrong season, thereby realizes factorial seedling growth, promotes the commercialization of cotton seedling.In a word, cotton joint position cottage propagation technology to cotton scientific worker and cotton grower, has important application.
Cotton is the crops that rely on seed to breed always, the rare report of the application study of vegetative propagation on cotton.The cottage propagation technology of cotton (the Chinese cotton of Qu Jianmu [J] in 1981,1985(3) 29-30) etc. has reported that the vegetative shoot cuttage of cotton can survive under the suitable condition, but has not seen into the seedling report.The good grade of Wu Zhen utilizes cotton stem stem apex and part nutrition organs to test, but planting percent is very low.Jing Shue " cuttage technique in the cotton distant hybridization " [Chinese cotton, 1985.4] test, though upland cotton can Cheng Miao, test is carried out in the greenhouse, and does not see the results seed.
Sichuan Noah seed bio tech ltd utilizes perennial root to carry out vegetative propagations such as cotton kernel sterility dual serial, its method application patent, application number/patent No. 2007100496562, its content is behind the results seed, all cotton plants are stayed in the cotton field and are survived the winter naturally, grow regrowth in the coming year again on the perennial root, utilize regrowth to breed; China's Cotton Research Institute " a kind of cuttage breeding method of cotton ", application number/patent No. 2005100889647, its method is that the cotton branch cutting is expanded numerous taking root in cotton regenerated seedling rooting medium, or it is the base portion of cotton branch is first with cotton branch root-growing agent immersion 20~28 hours, culture of rootage is carried out in cuttage in Cultivation of Cotton Seedlings Without Soil matrix again, treat to transplant after the cotton branch grows root system, obtain cotton plants.More than experiment can only be in the laboratory, in fluorescent lamp (illumination), adapt circumstance (temperature, humidity) and culture medium condition control down, part can Cheng Miao, all can not once finish in the production of land for growing field crops.And since the cost height, production application in a large number.Utilize joint position cuttage technique, the research of land for growing field crops fast asexual propagation cotton seeds, not seeing as yet both at home and abroad has successfully report.
Summary of the invention
Technical problem to be solved by this invention provides a kind of new cotton asexual reproduction method, and topmost other method that is different from of this method is; 1. do not need cotton regenerated seedling rooting medium, also do not need root-growing agent to soak, but all cuttages of cotton ground also can be saved cost; 2. whole process does not need the greenhouse, can once finish big Tanaka; 3. do not need Artificial Control temperature, humidity,, utilize natural environment can become seedling behind the overlay film in cotton growth season; 4. need not specify the branch at a certain position, stem joint position no matter, fruit branch joint position, leaf branch joint position, but equal cuttage Cheng Miao; 5. cost is low, and planting percent is up to more than 98%.
The technical scheme that the present invention takes is: the axil of each sheet leaf of cotton all has a bud, can have two or more axillalry buds simultaneously on each joint position.The axillalry bud of cotton can be divided into active bud and resting bud by its physiological activity state.Resting bud by physiological arousal, also can be transformed into active bud under certain condition.Utilize this biological property of cotton, after taking certain measure, make each joint position, develop into strain, can grow normally, thereby provide a kind of new method for vegetative propagation technique.
The present invention is to provide a kind of brand-new cotton vegetative propagation technique, grow season, adopt cotton plant stem joint position or fruit branch joint position at cotton growth, the whole leaf branch that goes in the time of also can utilizing training, press a joint position clip branch with secateurs during cuttage, be cut into the cuttage branch of long 5~8cm, 1 joint, 1 leaf; The seedbed can be selected in the location of hidding and sunny side, and shallow cut is generally 8~10cm, meticulous whole ground, makes soil soft, the railway carriage or compartment is wide to be generally 1~1.2 meter, to disinfecting soil, saturated with upper soll layer with the 0.5% carbendazim aqueous solution, water content is exosmosed with nothing and is advisable, and soil is divided into fritter, generally can divide 7cm 2, can cuttage be in soil one by one with the cuttage branch, burying deeply is as the criterion a little more than native face with the joint position, and about 1cm, the arch film covers above cuttage seeding, uses dirt seal all around, to keep temperature and humidity.The Ruo Riping temperature reaches 28 when spending, and can add a cover the sunshade net on the arch film, makes axillary bud sprouting on its cuttage branch, all grows young leaves later in 10 days, and 15 days begin to take off the film hardening later on, transplanted to the land for growing field crops later in 20 days.Land for growing field crops training pipe is identical with conventional cultivation.Its transplanting planting percent of the cuttage seeding that the method is cultivated reaches about 98%.
Indication cuttage branch among the present invention, transplant a cutting be meant cut from cotton plants and be cut into the branch that certain-length is used for cuttage, cuttage seeding is meant the cuttage branch through cultivating, taken root and branch on axillary bud sprouting grow young leaves and the cotton seedling of some root systems arranged.
By the evidences such as medicament of taking root of cotton plant different parts joint position, a different joints position sample length, the different cuttage degree of depth, difference:
1. the cotton of different length is saved position plugged ear (branch), can both cutting survival, planting percent is best with the long plugged ear of 5 ㎝ and 8 ㎝, crossbreed is better than conventional the kind in the high and new number of sheets investigation of new stem of cuttage Cheng Miaohou, in the trial stretch in the same kind plugged ear long to be better than plugged ear short.
2. chemicals treatment is little to the plugged ear survival rate influence of cotton joint position.The inventor has carried out the chemicals treatment comparative trial before to cuttage, and medicament A is that root-growing agent, medicament B are that the spirit of many mattresses, medicament C are thiophanate methyl, and survival rate all is 100%, and the clear water control treatment is 97%, and survival rate all reaches a high level.But chemicals treatment is heavy influential to cotton shoot root number, root.Experiment shows that medicament A is the most obvious to joint position cuttage root-taking effect, and medicament B has positive effect to a cotton joint position cuttage rooting, but after medicament ⑶ handled, individual plant radical, the heavy effect of root were not as the contrast clear water.
3. but the cotton stem saves position, leaf branch joint position, a fruit branch joint even cuttage Cheng Miao, but aspect root quantity, surface area, length, a leaf branch joint position plugged ear behaves oneself best, and the stem plugged ear is the poorest, but a stem joint plugged ear root diameter is the thickest.
4. the land for growing field crops performance of different parts joint position cuttage seeding is analyzed, and the parent strain has direct influence to the economical character and the economic characters of joint position plugged ear seedling; The joint position plugged ear cotton plant of different parts is high and becomes bell to count difference with insignificance in the same parent strain, and the fruit branch number is remarkable with joint number difference really.
5. by biological yield and economic flow rate behind the cuttage seeding transplanting land for growing field crops, cotton joint position be comparison shows that, a cotton joint position cottage propagation has good practical value.
The invention has the beneficial effects as follows: cotton joint position cottage propagation technology is compared with seed or other laboratory nutrition organs modes of reproduction, has the following advantages:
1.. but each joint position is Cheng Miao all, and planting percent is up to about 98%.
2.. no matter upland cotton or sea-island cotton, middle cotton, cotton, colored cotton, hybrid cotton, conventional variety Cottonopolis are suitable for the method.
3.. process operation is easy, does not need special implementation condition (laboratory, greenhouse).The whole operation process just can be finished smoothly big Tanaka.
4.. reproduction coefficient is big, can gather in the crops more seed.
5.. every 667m 2Can produce gined cotton more than 50 kilograms.
6.. low production cost, easy and simple to handle, because of not needing to use root-growing agent etc., can save cost greatly.
From following description, those skilled in the art can be well understood to purpose of the present invention and embodiments of the present invention:
1.. in generally more than cotton plant has 30 joint positions (comprising stem), breed quantity as required, every strain can be got a certain amount of branch (also can get complete stool every strain) respectively, becomes (5~8cm is long) to transplant a cutting with secateurs by joint position (keeping 1 leaf) straight snips.
2.. the finishing seedbed, in the location of experimental field choosing hidding and sunny side, (8~10cm), thin whole, to disinfecting soil, water-sprinkling is divided into 7cm to soil saturation with marker to shallow cut with 0.5% carbendazim soup 2Blockage.
3.. cuttage: cut by the joint position, the skewer Tiaokou is flat, is not the inclined-plane, and the long 5~8cm in every joint position carries out cuttage with the cutting of clip, is standard with the joint position a little more than soil surface.
4.. the arch film covers, and after cuttage is finished, covers with the arch film immediately, begins hardening after 15 days, must not take off film midway.
5.. transplant: get seedling by the nutritive cube seedling raising manners, density can be slightly larger than planting density in normal season.
6.. required asexual propagation material, can breed in the field.Can carry out field management by cultivating cotton normal season after the transplanting, also can utilize plastic film mulching cultivation, to promote the cotton nutrition growth.
Embodiment
Embodiment:The inventor finds the outstanding variant of 1 strain in the cotton breeding process, be accelerates multiplication, gets its all leaf branches and part fruit branch, saves a cuttage, promptly obtains more seed then.
In April, 2009, the declarer finds that in Hainan 1 strain has the genetic variation strain of the value utilized very much, because of being in vegetative growth phase and reproductive stage at that time, the seed of can't gathering.Promptly take to cut the part branch,, take back the Hunan in the plastic sack of packing into the wet towel parcel.In the location of experimental field choosing hidding and sunny side, (8~10cm), thin whole, to disinfecting soil, water-sprinkling is divided into 7cm to soil saturation with marker to shallow cut with 0.5% carbendazim water liquid 2Blockage.Then the branch taken back is cut by the joint position, the skewer Tiaokou is flat, is not the inclined-plane, totally 15 joint positions, every joint long 5~8cm in position, will save a bar respectively skewer go into off-the-shelf each square central authorities.After cuttage is intact, cover, compress sealing with earth all around with film arch film.All grew young leaves in 10 days later on, 15 days begin to take off the film hardening later on, transplanted to the land for growing field crops later in 20 days.Land for growing field crops training pipe is identical with conventional cultivation.
Whole growth process is identical with the conventional cultivation kind, and real 8 on the bell of receiving of individual plant is gathered in the crops grain surplus the seed 1000 then.
For the present invention, be to utilize cotton joint position (root, other organ do not belong to this category), carry out cuttage and seedling culture, breeding seed and production.
Though the present invention only provides 1 embodiment, should be appreciated that for other Gossypium kind and save a cuttage that suitable change is arranged, and this point it will be apparent to those skilled in the art that.

Claims (4)

1. the joint position cottage propagation technology that grows cotton is characterized in that: grow season at cotton growth, adopt cotton plant stem joint position or fruit branch, leaf branch joint position, be cut into the cuttage branch of long 5~8cm, cuttage is in soil one by one for 1 joint, 1 leaf, the joint position that will take off then, and the arch film covers above cuttage seeding soil, keep temperature and humidity, make axillary bud sprouting on its cuttage branch, develop into seedling, treat that seedling length is behind certain-length, carry out hardening, transplant.
2. according to claim 1 one grows cotton saves position cottage propagation technology, and it is characterized in that: to disinfecting soil, soil moisture content is exosmosed with nothing and is advisable with the 0.5% carbendazim aqueous solution.
3. according to claim 1 one grows cotton saves position cottage propagation technology, it is characterized in that: behind arrangement soil, the soil of putting in order is divided into blockage with marker, and each square central authorities, skewer is gone into 1 joint position bar.
4. according to claim 1 one grows cotton saves position cottage propagation technology, it is characterized in that: branch is cut by the joint position, requires the skewer Tiaokou concordant, is not the inclined-plane.
CN 201110131903 2011-05-20 2011-05-20 Node cutting propagation technology for cotton Pending CN102257919A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102792839A (en) * 2012-08-15 2012-11-28 大英天骄高新棉产业有限公司 Method for cultivating upland cotton
CN104322261A (en) * 2014-11-13 2015-02-04 张家港耕耘家庭农场有限公司 Efficient rosa chinensis propagation method
CN104705051A (en) * 2015-02-10 2015-06-17 南阳市农业科学院 Cotton seedling transplantation method
CN105557500A (en) * 2015-12-02 2016-05-11 中国农业科学院棉花研究所 Cotton aneuploid construction method
CN107006247A (en) * 2017-03-24 2017-08-04 合肥云都棉花有限公司 One cottage method grown cotton

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1907001A (en) * 2005-08-03 2007-02-07 中国农业科学院棉花研究所 Cuttage reproducing method for cotton

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1907001A (en) * 2005-08-03 2007-02-07 中国农业科学院棉花研究所 Cuttage reproducing method for cotton

Non-Patent Citations (3)

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Title
《中国优秀硕士学位论文全文数据库》 20110315 周毅 棉花节位扦插繁殖技术初探 10-14 1-4 , 第3期 *
《北京农业》 20110228 周贤强 影响棉花扦插成活的内部因素研究 54-55 1-4 , 第6期 *
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102792839A (en) * 2012-08-15 2012-11-28 大英天骄高新棉产业有限公司 Method for cultivating upland cotton
CN104322261A (en) * 2014-11-13 2015-02-04 张家港耕耘家庭农场有限公司 Efficient rosa chinensis propagation method
CN104705051A (en) * 2015-02-10 2015-06-17 南阳市农业科学院 Cotton seedling transplantation method
CN105557500A (en) * 2015-12-02 2016-05-11 中国农业科学院棉花研究所 Cotton aneuploid construction method
CN105557500B (en) * 2015-12-02 2018-06-08 中国农业科学院棉花研究所 A kind of method for building cotton aneuploid
CN107006247A (en) * 2017-03-24 2017-08-04 合肥云都棉花有限公司 One cottage method grown cotton

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Application publication date: 20111130