CN108925261A - A kind of black fruit fructus lycii micro cuttage rapid propagation method - Google Patents
A kind of black fruit fructus lycii micro cuttage rapid propagation method Download PDFInfo
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- CN108925261A CN108925261A CN201810607486.3A CN201810607486A CN108925261A CN 108925261 A CN108925261 A CN 108925261A CN 201810607486 A CN201810607486 A CN 201810607486A CN 108925261 A CN108925261 A CN 108925261A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to plant cuttage propagation technical fields to lead to the problem that breeding coefficient is low, provide a kind of black fruit fructus lycii micro cuttage rapid propagation method to solve current black fruit fructus lycii cottage method in the presence of being limited by season and cuttings material etc..It selects management after maternal plant, nursery, cuttage, cuttage, hardening, play transplantation of seedlings, non-woven fabrics Seedling bag carries out nursery, and seedling medium is mixed in proportion by turf, vermiculite, perlite.Retain one bud of a leaf or two leaves, one bud on the new shoot of maternal plant as cuttings material（Length is 1.5-2.0cm）；In 45 DEG C of bevelings at bud section lower end 1-1.5cm, the truncation at bud section upper end 0.5cm；Matrix is poured to drenched with the EM bacterium solution dilution that black jade powder is added on the day before cuttage；Rooting process is completed after in cuttage 12d（Root length 1cm）, root growth grows in 20d, into the hardening phase.The time for shortening cuttage rooting increases the survival rate that the later period transplants seedlings；Survival rate reaches 95%.
Description
Technical field
The invention belongs to plant cuttage propagation technical fields, and in particular to a kind of side that black fruit fructus lycii micro cuttage is quickly bred
Method.
Background technique
Black fruit fructus lycii（Lycium ruthenicum Murr.）For Solanaceae（Solanaceae）Lycium（Lycium）It plants
Object is a kind of wild more quil shrubs, is mainly distributed on western China.Fruit is sweet in flavor, mild-natured, is effective natural antioxygen
Agent, the nutrient composition contents such as vitamin, minerals are also very abundant, can be used as medicine；Plant can also check winds and fix drifting sand, it is saline and alkaline to administer
Ground.Black fruit fructus lycii product is mainly from wild lycium ruthenicum, wild in recent years since the deterioration of growing environment and people excessively acquire
Production-goods source receives a degree of destruction.Therefore, protection and exploitation benefit of the expanding propagation method to its resource of black fruit fructus lycii are probed into
Use important in inhibiting.
It is the main numerous mode of expansion that black fruit fructus lycii, which takes cutting propagation, and black fruit fructus lycii can both have been allowed to keep maternal Optimality
Shape, moreover it is possible to Speedup of reproduction and breeding speed.The upper widely used predominantly shoot cutting method of production at present, can substantially meet needs
It asks, but still will receive the limitation in season and cuttings material, influence its breeding coefficient, so being necessary to adopt breeding in production
The higher cottage method of coefficient-micro cuttage method carries out nursery breeding.
Micro cuttage method refers in the case where being not necessarily to or only needing a small amount of Hormone Conditions, the stem section of 1 bud of in vitro culture band（Single stipes
Or miniature cutting）, make terminal bud or axillary bud development seedling.Although this method belongs to a kind of method of shoot cutting, but it herein in connection with
The advantage of tissue culture technique has the characteristics that breeding coefficient is high, cultivation cycle is short and environmental condition is easy to control.
Black jade is a kind of precious and rare natural resources, can generate black jade powder in process, and the latter equally accumulates
26 kinds of calcic, magnesium, iron, zinc, selenium etc. the microelements beneficial to human body（Content meets national soil quality standard GB19618-
1995）.After ad hoc fashion is handled, black jade powder can effectively discharge itself microelement, so it is proportionally added
Into seedling medium, microelement-supplementing can be played the role of, cuttings root system is promoted to sprout and grow.According to Different Crop
The method of characteristic, micro cuttage is had nothing in common with each other.
Summary of the invention
The present invention causes to solve current black fruit fructus lycii cottage method in the presence of being limited by season and cuttings material etc.
The low problem of breeding coefficient provides a kind of black fruit fructus lycii micro cuttage rapid propagation method.
The present invention is realized by following technical solution：A kind of black fruit fructus lycii micro cuttage rapid propagation method, select maternal plant,
Management, hardening, transplantation of seedlings after nursery, cuttage, cuttage, specific step is as follows：
（1）Select maternal plant：1-2 is raw, shape is uniform plant is selected as maternal plant, after growth period in plant strain growth season
Even root is dug out, and is transplanted in greenhouse；To trimming branch after seedling-slowing stage, so that plant quickly sends forth branches item；To new branch
It is long to 15-25cm when, be ready for micro cuttage；
（2）Nursery prepares：Non-woven fabrics Seedling bag is packed into seedling medium and carries out nursery, and the Seedling bag for installing seedling medium is emitted into
In seedling bed lower than ground 8-10cm, arched shed is built above Seedling bag, high pressure sprays fog equipment, spraying device are installed at the top of arched shed
Connect water tank；The shading net of film and 70% is set up above spraying device；
Seedling medium is handled on the day before cuttage：10g black jade powder is added in the EM bacterium solution of 100ml and stirs soaked overnight after 30min, skewer
Inserting the previous day dilutes 100-200 times for the EM bacterium solution that black jade powder is added and pours matrix to drenched；Control seedling medium temperature is 20-
28℃；
（3）Cuttage：The new shoot for selecting 20-25cm on maternal plant, using the stem with bud of 1.5-2cm long as a cuttings material
Material, each stem section retain one bud of a leaf or two leaves, one bud；In 45 ° of bevelings at bud section lower end 1.0-1.5cm, on bud section
Hold truncation at 0.5cm；
After cuttings is cut, by cuttings lower end in prepared solution A quickly dipped 3-5s, be then inserted directly into Seedling bag, one
Cuttings uses a Seedling bag；The blade of cuttings is close to seedling medium surface when cuttage, and cuttings depth is 1-1.5cm；Cuttage is complete
At Seedling bag again with prepare solution B sprayed；After cuttage whole, film, shading net are put up, and opened spraying
Device；
Wherein：Solution A is HORMONE TREATMENT liquid, is formulated and is：Indolebutyric acid 300-500ppm, methyl α-naphthyl acetate 60-500ppm, sulfalene are disliked
Azoles 50-100ppm；The concentration of heteroauxin and methyl α-naphthyl acetate ratio is 1：1-5：1；Solution B is sulfamethoxazole 50-100ppm；
（4）It is managed after cuttage：After cuttage in arched shed air temperature control at 25-30 DEG C；Early period：5-12d after cuttage is wet in arched shed
Degree control is in 90-95%, and spray 15s is spraying within every 20 minutes；The stage uses shading net, and control intensity of illumination is 4000-5000Lx；
The coolant-temperature gage used by spraying is >=15 DEG C；Mid-term：After cuttage in 13-20d, humidity is 85-90%, every 30 minutes spray 15s in arched shed
It is spraying；Light control is in 4000-6000Lx；Cloudy day opens shading net, supplementary light；Later period：After cuttage 20d, cuttings root is raw
Length is vigorous, spraying device, plastic foil and shading net is removed, into the hardening phase；
（5）Hardening：After cuttage 20d, sprinkling watering keeps seedling medium humidity 70%；Fine day is early, divulges information afternoon, and divulge information 10- every time
20min；Cloudy day ventilation 5-10min or stuffy；After cuttings sends forth branches item and when length reaches 5-10cm, hardening phase knot
Beam；
（6）Play transplantation of seedlings：After the hardening phase, the suitable time is selected to arrive the black fruit fructus lycii transplantation of seedlings in non-woven fabrics Seedling bag
Big Tanaka.
Specifically hardening off method is：Spring and summer autumn：After cuttage 20d, sprinkling watering keeps seedling medium humidity 70%；Fine day exists
Early 10-11 point, the ventilation of evening 4-5 point, divulged information 15-20min every time, every 3-4 days increase 5-10min；Cloudy ventilation time 5-
10min is stuffy；After cuttings sends forth branches item and when length reaches 5-10cm, the hardening phase terminates；
Winter：After cuttage 20d, watering keeps 70% seedling medium humidity；Fine day is divulged information in early 11-12 point, 3-4 point in afternoon, often
Secondary ventilation 10-15min, every 5 days increase 5min；Cloudy ventilation time is 5-10min or stuffy；When cuttings sends forth branches
After item and when length reaches 5-10cm, the hardening phase terminates.
High pressure sprays fog equipment is set at 60cm above Seedling bag.The seedling medium is by turf, vermiculite, perlite according to weight
Amount is than being 1:1:1 is mixed with.The non-woven fabrics Seedling bag diameter is 8cm, a height of 12cm；Seedling medium in Seedling bag
Depth is 10cm.The film is plastic foil, with a thickness of 0.08mm.Made by spraying after water storage 12-24h in the water tank
With.When cuttage, cuttings is separated to cuttage from plant and completes, and the time controls in 8-10s.
It is preferred that：Solution A is HORMONE TREATMENT liquid, is formulated and is：Indolebutyric acid 400ppm, methyl α-naphthyl acetate 100ppm, sulfamethoxazole
100ppm；The concentration of heteroauxin and methyl α-naphthyl acetate ratio is 4:1；Solution B is sulfamethoxazole 100ppm.
The black jade powder that the present invention uses is the waste that black jade processing generates, and can give Rapid Rooting and the growth of cuttings
Microelement more abundant is provided, there are preferable social benefit and ecological benefits.Cuttings early period can be given using Seedling bag
It takes root and grows and sufficient nutrition is provided, facilitate growing up strong and sturdy for plant after transplanting in this way；It can be directly by nursery when transplanting
Bag buries big Tanaka, avoids seedling root and is damaged, improves survival rate.
The micro cuttage method seedling raise period that the present invention uses is short, high survival rate.The band bud cutting fringe of 1.5-2.0cm is in cuttage 7-8d
After can complete rooting process, shorten the time of cuttage rooting, accelerate the speed of root growth；Nutrition is filled in Seedling bag
Foot, the root growth of cuttings is vigorous, enters the hardening phase after 20d after cuttage, only needs normal management later, save people
Power material resources；Cuttings grow up strong and sturdy and the use of Seedling bag increases the survival rate that the later period transplants seedlings, survival rate reaches 95%.
Specific embodiment
Embodiment 1：The present embodiment winter in Taiyuan region greenhouse breeds, and material is the stingless of the northern weather of adaptation
Black fruit fructus lycii.
A kind of black fruit fructus lycii micro cuttage rapid propagation method, select management after maternal plant, nursery, cuttage, cuttage, hardening,
Transplantation of seedlings is played, specific step is as follows：
（1）Select maternal plant：In the black fruit fructus lycii season of growth, 5-10 plant shape shape good stand is picked out（Biennial plant）, property
Shape shows as plant type is compact, stingless and fruit is big etc..After growth period（November）Even root is dug, and is transplanted in greenhouse.To slow
After seedling stage, branch is trimmed, so that the maternal plant item that quickly sends forth branches can start to carry out when new branch grows to 15-25cm
Micro cuttage.Cutting time selection early, evening preferably.
（2）Nursery prepares：Non-woven fabrics Seedling bag is packed into seedling medium and carries out nursery, and Seedling bag specification is diameter 8cm, height
12cm；The Seedling bag for installing seedling medium is emitted into the seedling bed lower than ground 8-10cm, builds arched shed above Seedling bag,
Small plastic shed is built and is the epidemic disaster in order to preferably control seedling raising environment, the width and length of arched shed are according to greenhouse actual conditions
Depending on, high pressure sprays fog equipment is installed, spraying device connects water tank, and the water in water tank needs to store ability after 12-24h at the top of arched shed
It uses, avoids impacting root growth because water temperature is too low.The shading net of film and 70% is set up above spraying device；
Seedling medium is 1 according to weight ratio by turf, vermiculite, perlite:1:1 is mixed with, deep-controlled in 10cm.
Seedling medium is handled on the day before cuttage：It was impregnated after 10g black jade powder stirring 30min is added in the EM bacterium solution of 100ml
The EM bacterium solution that black jade powder is added is diluted 100-200 times on the day before cuttage and pours matrix to drenched by night；Base will be guaranteed by pouring for the first time
Matter is drenched completely.EM bacterium solution is conventional commercial EM bacterium solution.Controlling seedling medium temperature is 20-28 DEG C, can promote cuttings root
Fast-growth.
EM bacterium solution contains the benefit materials being largely easily absorbed by plants, such as amino acid, organic acid, Polysaccharides, the life of various dimensions
Plain, various biochemical enzymes, somatomedin, antibiotic and antiviral substance etc., in addition the lot of trace being dissolved into from black jade powder
Element, the root that EM bacterium solution that treated can effectively facilitate cuttings is sprouted and growth, obtains the disease-free good seed of health.
（3）Cuttage：The new shoot for selecting 20-25cm on maternal plant is inserted using the stem with bud of 1.5-2cm long as one
Fringe material, each stem section retain one bud of a leaf or two leaves, one bud；In 45 ° of bevelings at bud section lower end 1.0-1.5cm, apart from bud
Save truncation at the 0.5cm of upper end；It is preferred that green branch, is sheared using knife blade, movement will guarantee notch rapidly when shearing
Neatly, mechanical damage suffered by cuttings notch is reduced.
Cuttings processing：After cuttings is cut, by cuttings lower end in prepared solution A quickly dipped 3-5s, be then inserted directly into
In Seedling bag, a cuttings uses a Seedling bag；This process is resolute rapidly, and cuttings is separated to cuttage success from plant, when
Between control in 8-10s.The blade of cuttings is close to seedling medium surface when cuttage, and cuttings depth is 1-1.5cm, cuttings insertion section
Dividing will be in close contact with matrix.The Seedling bag that cuttage is completed is sprayed with the solution B prepared again；It, will after cuttage whole
Plastic foil, shading net are put up, and open spraying device；
Wherein：Solution A is HORMONE TREATMENT liquid, is formulated and is：Indolebutyric acid 400ppm；Methyl α-naphthyl acetate 100ppm；Sulfamethoxazole
100ppm；The concentration of heteroauxin and methyl α-naphthyl acetate ratio is 4：1；Solution B is sulfamethoxazole 100ppm.
（4）It is managed after cuttage：Air temperature control is at 25-30 DEG C in arched shed after cuttage, if temperature is more than 30 DEG C, and
When adjust plastic foil and shading net covering degree, carry out ventilation cooling.Early period：5-12d after cuttage, cuttings is in root system at this time
In sprouting period, humid control is in 90-95% in arched shed, and spray 15s is spraying within every 20 minutes；The stage uses shading net, and control illumination is strong
Degree is 4000-5000Lx；The coolant-temperature gage used by spraying is >=15 DEG C；Mid-term：After cuttage in 13-20d, cuttings is grown up in root system
Period.Humidity is 85-90% in arched shed, and spray 15s is spraying within every 30 minutes；Light control is in 4000-6000Lx；Cloudy day opens shading
Net, supplementary light；Later period：After cuttage 20d, cuttings root is flourishing, spraying device, plastic foil and shading net is removed, into hardening
Phase；
（5）Hardening：Winter：After cuttage 20d, watering keeps 70% seedling medium humidity.Fine day can be in early 11-12 point, 3- in afternoon
4 points were aerated, and divulge information 10-15min every time, every 5 days increase 5min；Cloudy day reduces 5-10min of ventilation time or obstructed
Wind.After cuttings sends forth branches item and when length reaches 5-10cm, the hardening phase terminates.
（6）Play transplantation of seedlings：After the hardening phase, stingless black fruit fructus lycii seedling well developed root system in non-woven fabrics Seedling bag, growth
It is healthy and strong, select the suitable time to be transplanted to big Tanaka in the second year spring, survival rate reaches 95%.
Experimental example 1：By micro cuttage method of the invention（A）With traditional shoot cutting method（B）It is compared, observation is inserted
Fringe is taken root situation.It is tested using stingless black fruit fructus lycii as material, two groups carry out in same Small plastic shed, and every group of cuttings is 100
A, from start recording data after cuttage 5d, 9 points of every morning randomly selects 10 cuttings and is investigated.Experimental result is shown in Table 1.
The growing state of the different cottage methods of table 1 compares
The results show that herein described method can be improved the Rapid Rooting of cuttings, seedling raise period is short, accelerates root growth
Speed and vigorous degree, rooting percent 92%.
Experimental example 2：Chosen respectively in two groups of experiments in experimental example 1 the consistent healthy cuttings seedling of 50 plants of growth conditions into
Row transplanting experiment, every 15d is observed after transplanting.It randomly selects 10 cuttings 9 points of every morning and carries out height of seedling and new slightly length
The statistics of variation counts the survival rate of whole transplanted seedlings in 60d.The height of seedling of black fruit fructus lycii seedling and new slightly length growing state point
It is not shown in Table 2 and table 3, the results show that herein described method can be improved the speed of growth of the cuttings after transplanting, growth rate is much
Higher than traditional shoot cutting, survival rate is up to 95.1%.
The Seedling height growth variation of fructus lycii seedling after table 2 is transplanted
The growth change of fructus lycii seedling newly slightly after table 3 is transplanted
Claims (9)
1. a kind of black fruit fructus lycii micro cuttage rapid propagation method selects maternal plant, management after nursery, cuttage, cuttage, is risen hardening
Transplantation of seedlings, it is characterised in that：Specific step is as follows：
（1）Select maternal plant：1-2 is raw, shape is uniform plant is selected as maternal plant, after growth period in plant strain growth season
Even root is dug out, and is transplanted in greenhouse；To trimming branch after seedling-slowing stage, plant quickly sends forth branches item；It is long extremely to new branch
When 15-25cm, it is ready for micro cuttage；
（2）Nursery prepares：Non-woven fabrics Seedling bag is packed into seedling medium and carries out nursery, and the Seedling bag for installing seedling medium is emitted into
At the 8-10cm of ground, arched shed is built above Seedling bag, high pressure sprays fog equipment is installed at the top of arched shed, spraying device connects water
Case；The shading net of film and 70% is set up above spraying device；
Seedling medium is handled on the day before cuttage：10g black jade powder is added in the EM bacterium solution of 100ml and stirs soaked overnight after 30min, skewer
Inserting the previous day dilutes 100-200 times for the EM bacterium solution that black jade powder is added and pours matrix to drenched；Control seedling medium temperature is 20-
28℃；
（3）Cuttage：The new shoot for selecting 20-25cm long on maternal plant, using the stem with bud of 1.5-2cm long as a cuttings
Material, each stem section retain one bud of a leaf or two leaves, one bud；In 45 DEG C of bevelings at bud section lower end 1.0-1.5cm, apart from bud
Save truncation at the 0.5cm of upper end；
After cuttings is cut, by cuttings lower end in prepared solution A quickly dipped 3-5s, be then inserted directly into Seedling bag, one
Cuttings uses a Seedling bag；The blade of cuttings is close to seedling medium surface when cuttage, and cuttings depth is 1-1.5cm；Cuttage is complete
At Seedling bag again with prepare solution B sprayed；After cuttage whole, film, shading net are put up, and opened spraying
Device；
Wherein：Solution A is HORMONE TREATMENT liquid, is formulated and is：Indolebutyric acid 300-500ppm, methyl α-naphthyl acetate 60-500ppm, sulfalene are disliked
Azoles 50-100ppm；The concentration of heteroauxin and methyl α-naphthyl acetate ratio is 1：1-5：1；Solution B is sulfamethoxazole 50-100ppm；
（4）It is managed after cuttage：After cuttage in arched shed air temperature control at 25-28 DEG C；Early period：5-12d after cuttage is wet in arched shed
Degree control is in 90-95%, and spray 15s is spraying within every 20 minutes；The stage uses shading net, and control intensity of illumination is 4000-5000Lx；
The coolant-temperature gage used by spraying is >=15 DEG C；Mid-term：After cuttage in 13-20d, humidity is 85-90%, every 30 minutes spray 15s in arched shed
It is spraying；Light control is in 4000-6000Lx；Cloudy day opens shading net, supplementary light；Later period：After cuttage 20d, cuttings root hair
It reaches, spraying device, plastic foil and shading net is removed, into the hardening phase；
（5）Hardening：After cuttage 20d, sprinkling watering keeps seedling medium humidity 70%；Fine day is early, divulges information afternoon, and divulge information 10- every time
20min；Cloudy day ventilation 5-10min or stuffy；After cuttings sends forth branches item and when length reaches 5-10cm, hardening phase knot
Beam；
（6）Play transplantation of seedlings：After the hardening phase, the suitable time is selected to move the stingless black fruit fructus lycii seedling in non-woven fabrics Seedling bag
Plant big Tanaka.
2. a kind of black fruit fructus lycii micro cuttage rapid propagation method according to claim 1, it is characterised in that：Specific hardening
Method is：Spring and summer autumn：After cuttage 20d, sprinkling watering keeps seedling medium humidity 70%；Fine day is in early 10-11 point, evening 4-5 point
Ventilation, divulged information 15-20min every time, every 3-4 days increase 5-10min；Cloudy ventilation time 5-10min or stuffy；When slotting
When fringe sends forth branches after item and length reaches 5-10cm, the hardening phase terminates；
Winter：After cuttage 20d, watering keeps 70% seedling medium humidity；Fine day is divulged information in early 11-12 point, 3-4 point in afternoon, often
Secondary ventilation 10-15min, every 5 days increase 5min；Cloudy ventilation time is 5-10min or stuffy；When cuttings is grown newly
After branch and when length reaches 5-10cm, the hardening phase terminates.
3. a kind of black fruit fructus lycii micro cuttage rapid propagation method according to claim 1, it is characterised in that：On Seedling bag
High pressure sprays fog equipment is set at square 60cm.
4. a kind of black fruit fructus lycii micro cuttage rapid propagation method according to claim 1, it is characterised in that：The nursery
Matrix is 1 according to weight ratio by turf, vermiculite, perlite:1:1 is mixed with.
5. a kind of black fruit fructus lycii micro cuttage rapid propagation method according to claim 1, it is characterised in that：The nonwoven
Cloth Seedling bag diameter is 8cm, a height of 12cm；Seedling medium depth in Seedling bag is 10cm.
6. a kind of black fruit fructus lycii micro cuttage rapid propagation method according to claim 1, it is characterised in that：The film
For plastic foil, with a thickness of 0.08mm.
7. a kind of black fruit fructus lycii micro cuttage rapid propagation method according to claim 1, it is characterised in that：The water tank
In water storage 12-24h after carry out spraying use.
8. a kind of black fruit fructus lycii micro cuttage rapid propagation method according to claim 1, it is characterised in that：When cuttage,
Cuttings is separated to cuttage success from plant, and the time controls in 8-10s.
9. a kind of black fruit fructus lycii micro cuttage rapid propagation method according to claim 1, it is characterised in that：Solution A is
HORMONE TREATMENT liquid, is formulated and is：Indolebutyric acid 400ppm, methyl α-naphthyl acetate 100ppm, sulfamethoxazole 100ppm；Heteroauxin and naphthalene second
The concentration ratio of acid is 4：1；Solution B is sulfamethoxazole 100ppm.
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| CN109496572A (en) * | 2018-12-25 | 2019-03-22 | 福建农林大学 | Japanese red maple Green Dragon softwood cutting propagation method |
| CN109997851A (en) * | 2019-04-09 | 2019-07-12 | 宁波市农业科学研究院 | A kind of xylophyta cuttage root-taking powder of anti-browning and preparation method thereof |
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