CN103583574A - Plant source smoke water and its preparation method and use in promotion of red sage root active-substance accumulation - Google Patents

Plant source smoke water and its preparation method and use in promotion of red sage root active-substance accumulation Download PDF

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CN103583574A
CN103583574A CN201310631035.0A CN201310631035A CN103583574A CN 103583574 A CN103583574 A CN 103583574A CN 201310631035 A CN201310631035 A CN 201310631035A CN 103583574 A CN103583574 A CN 103583574A
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cigarette
water
plant resource
red sage
cigarette water
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CN103583574B (en
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周洁
王晓
耿岩玲
刘伟
方磊
刘峰
段文娟
王岱杰
赵恒强
李奉胜
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Shandong Analysis and Test Center
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Abstract

The invention discloses plant source smoke water and its preparation method and use in promotion of red sage root active-substance accumulation. Dry fallen leaves of platanus occidentalis, platanus acerifolia and platanus orientalis are smoldered and the smoke produced by smoldering is fed into water so that the plant source smoke water is obtained. The invention also discloses a method for improving traditional Chinese medicine red sage root active-substance content by the plant source smoke water. The preparation method has simple and fast processes, can be realized easily, adopts withered and yellow fallen plant leaves as raw materials, realizes waste use, changes waste into valuables, has a low cost and is convenient for industrial production. The plant source smoke can obviously improve red sage root active-substance content, can improve a red sage root yield and has high economic benefits.

Description

The application in promoting the accumulation of red sage root active principle of a kind of plant resource cigarette water, preparation method and cigarette water
Technical field
The present invention relates to the preparation method of a kind of plant resource cigarette water and cigarette water, also relate to and use this cigarette water to promote traditional Chinese medicine---the method for red sage root active principle accumulation, belongs to environment and technical field of traditional Chinese medicines.
Background technology
Along with being filled with of " back to nature " trend, traditional Chinese medicine is more and more subject to people's attention, and international and domestic market also sharply increases the demand of traditional Chinese medicine.Current more than 80% traditional Chinese medicine derives from plantation, and therefore, the quality good or not of plantation medicinal material directly determines the clinical efficacy of Chinese medicine.In traditional Chinese medicine, active principle content is the important symbol of evaluating quality of medicinal material, and type of rearing and cultivation management measure have material impact to quality of medicinal material.Yet the problems such as the upper cultivation management of production is at present extensive, seriously polluted cause quality of medicinal material sharply to decline, need Developing Green ecologic planting badly, improve quality of medicinal material, guarantee clinical efficacy, therefore how research is optimized medicinal material type of rearing and is of great importance to improving traditional Chinese medicine quality.
Plant resource cigarette water refers to vegetable material (leaf of plant, stem etc.) is produced to cigarette through smoldering, then these cigarettes passed into the aqueous solution obtaining in water.There are some researches show, cigarette water can promote seed germination and improve grain, vegetables and fruits yield and quality, as improved corn, wheat Isoquant; Improve plant vigor and the fruit quality of the vegetables such as tomato, swordweed and soya bean, and in tested concentration range, do not find poisoning or genetoxic.Increasing data show that cigarette water is showing tremendous potential aspect agricultural and gardening.At present, the rare report of research that cigarette water is cultivated for traditional Chinese medicine, Zhou Jie etc. have delivered the article of " cigarette water extraction high woaded blue seedling Aboveground Biomass of Young and indigo accumulation " by name, and cigarette water is used for to 2 years raw herbaceous plant woaded blues of Cruciferae isatis indigoticacultivation in, by plant resource, be specifically passerina vulgaristhoday and themeda triandrathe cigarette water of acrial part burning gained is Forssk. for woaded blue seedling, and result shows that the cigarette water treatment of 1:500-1:1000 concentration can improve woaded blue Aboveground Biomass of Young and indigo content.This article is that the beginning has been started in the application of cigarette water in traditional Chinese medicine field, but this article has only been described passerina vulgaristhoday and themeda triandraforssk. the impact of cigarette water in source on woaded blue seedling, does not carry out detailed research to other plant source cigarette water in the application in traditional Chinese medicine field.
Summary of the invention
The object of this invention is to provide a kind of plant resource cigarette water, this cigarette water can significantly improve the accumulation of the active ingredient of the red sage root, significant to the raising of red rooted salvia quality.
Another object of the present invention is to provide the preparation method of above-mentioned plant resource cigarette water, and the method is simple, effective, can obtain fast required cigarette water.
Another object of the present invention is to provide the application of above-mentioned plant resource cigarette water in promoting the accumulation of red sage root active principle.
At present, cigarette water is less for the research of traditional Chinese medicine cultivation, inventor finds under study for action, the made cigarette water of different plant origins affects different on the accumulation of active principle in traditional Chinese medicine, and the cigarette water in same plant source also has very big-difference to the accumulation of active principle in different traditional Chinese medicines, therefore, for traditional Chinese medicine cultivation, needing further investigation to produce the plant resource cigarette water that is applicable to this medicinal material in cigarette water.
The present invention is through a large amount of screening experiments, obtained the plant resource cigarette water of applicable red sage root active principle accumulation, and specifically studied preparation method and the using method of this cigarette water, thereby for providing important technical support by plant resource cigarette water for red rooted salvia quality.The concrete technical scheme of the present invention is as follows:
A cigarette water, is characterized in that: plant resource is the dry fallen leaves of a ball plane tree, two ball plane trees and these three kinds of arbors of sycamore.
In above-mentioned plant resource cigarette water, the mass ratio of a ball plane tree, two ball plane trees and sycamore leaf is preferably 1:1-10:0.5-5.
In above-mentioned plant resource cigarette water, the mass ratio of a ball plane tree, two ball plane trees and sycamore leaf optimum is 1:1:1 or 1:5:2.
Plant resource cigarette water of the present invention is that the method that the cigarette by 5-7kg plant resource smoldering 40-60min is produced passes in 500 ml cold water makes.Described smoldering refers to that botanical material burns in the inadequate situation of oxygen, and plant resource is lighted rear fray-out of flame in the case, and the flames of anger, because not fully burning can be emitted a large amount of cigarettes.
The preparation method of plant resource cigarette water of the present invention, it is characterized in that comprising the following steps: the withered and yellow fallen leaves of selecting a ball plane tree, two ball plane trees and sycamore, naturally dry, by proportioning, add in closed container, light rear smoldering, the cigarette that smoldering produces, in pipeline passes into the collector that is added with water, obtains plant resource cigarette water.
Described smoldering refers to that plant resource lights afterwards because of the not enough fray-out of flame of oxygen, only relies on residual Mars to carry out the form of burn incompletely, in smoldering process, can produce a large amount of cigarettes.
In above-mentioned preparation method, adopt following method to carry out treatment effect to plant resource better: after fallen leaves nature dries, first to carry out following processing, then carry out smoldering: fallen leaves are deposited in black room, with ultraviolet lighting 1-2 days, then with the thermal current of 75-80 ℃, process 5-6h.Described thermal current is hot-air or hot oxygen-enriched air.
In above-mentioned preparation method, gained plant resource cigarette water lucifuge low temperature is preserved.
Cigarette glassware for drinking water of the present invention has the effect of good promotion effective component in red sage accumulation, using method is: above-mentioned plant resource Yan Shui is mixed according to the volume ratio of 1:500-2000 with water, obtain cigarette water diluent, cultivation process by described cigarette water diluent for red sage root plant, to promote the accumulation of effective component in red sage.Further, can within every 3 days, with cigarette water diluent, water the red sage root once, consumption is 200ml/1000g soil.Other types of rearing of the red sage root are with method is identical now .
Tool of the present invention has the following advantages:
1, cigarette water preparation method of the present invention is simple to operate, succinctly quick, is easy to realize, and is convenient to suitability for industrialized production.
2, cigarette water of the present invention is raw materials used is the withered and yellow fallen leaves of plant, makes a large amount of fallen leaves obtain rationally utilizing fully, has realized making full use of of refuse, turns waste into wealth, and cost is low.
3, cigarette water of the present invention is that brown color has the liquid of smoke to dark-brown, belongs to fully natural green extract, no side effects.
4, cigarette water of the present invention can obviously promote the content of active ingredient in the red sage root, can also improve red sage root output, and economic benefit is high.
Accompanying drawing explanation
Fig. 1 is the schematic diagram that the present invention prepares cigarette water equipment therefor;
In figure, 1, container, 2, lid, 3, dividing plate, 4, lighting-up tuyere, 5, hide cigarette lid 6, pipeline, 7, collector.
Embodiment
Below by specific embodiment, the present invention will be further elaborated, should be understood that, following explanation is only in order to explain the present invention, its content is not limited.
The present invention prepares cigarette water device used and comprises: the container 1 that holds fallen leaves, this container is the container made of iron of barreled, upper end open with lid 2, on lid, be with jug, in cavity in container, be provided with the lateral partitions 3 of placing plant resource, on dividing plate with ventilation hole, dividing plate is positioned at the bottom of container, on the sidewall of container bottom, have lighting-up tuyere 4, on lighting-up tuyere, with hiding cigarette, cover 5, in container upper portion side wall, connect the pipeline 6 of cigarette output, the other end of pipeline is connected with collector 7, in described collector, cold water is housed, on collector, be connected with the pipeline of ganging up with the external world, to keep accumulator pressure normal.In use, plant resource is put into container, build lid, from lighting-up tuyere igniting, light plant resource, after igniting, with hiding cigarette lid, lighting-up tuyere is covered, prevent that cigarette from overflowing from lighting-up tuyere.The plant resource of lighting is because oxygen is not enough, so can not burn fully, fire can extinguish very soon, only stays Mars, and plant resource only can burn by Mars, now can produce a large amount of cigarettes, and the cigarette of generation enters in collector by pipeline, thereby becomes cigarette water.The present invention also can adopt other viable means in prior art to produce cigarette.
embodiment 1
Collect deciduous tree one ball plane tree platanus occidentalis; Two ball plane trees platanus acerifolia;sycamore platanus orientaliseach 5.5kg of withered and yellow fallen leaves, removes various impurity, cleans, and it is dried naturally, according to the ratio of mass ratio 1:1:1, packs in container as described in Figure 1, lights smoldering.After burning, produce cigarette and pass in the collector that 500ml cold water is housed of getting ready in advance, the cigarette that burning 40min produces passes in 500 ml cold water and makes plant resource cigarette water of the present invention, also can be described as cigarette jellyfish liquid.
embodiment 2
Collect deciduous tree one ball plane tree platanus occidentalis; Two ball plane trees platanus acerifolia;sycamore platanus orientaliseach 6kg of withered and yellow fallen leaves, removes various impurity, cleans, and it is dried naturally, according to the ratio of mass ratio 1:1:1, packs in container as described in Figure 1, lights smoldering.After burning, produce cigarette and pass in the collector that 500ml cold water is housed of getting ready in advance, the cigarette that burning 45min produces passes in 500 ml cold water and makes plant resource cigarette water of the present invention, also can be described as cigarette jellyfish liquid.
embodiment 3
Collect deciduous tree one ball plane tree platanus occidentalis; Two ball plane trees platanus acerifolia;sycamore platanus orientaliseach 7kg of withered and yellow fallen leaves, removes various impurity, cleans, and it is dried naturally, according to the ratio of mass ratio 1:1:1, packs in container as described in Figure 1, lights smoldering.After burning, produce cigarette and pass in the collector that 500ml cold water is housed of getting ready in advance, the cigarette that burning 50min produces passes in 500 ml cold water and makes plant resource cigarette water of the present invention, also can be described as cigarette jellyfish liquid.
embodiment 4
Collect deciduous tree one ball plane tree platanus occidentalis; Two ball plane trees platanus acerifolia;sycamore platanus orientaliseach 7kg of withered and yellow fallen leaves, removes various impurity, cleans, and it is dried naturally, according to the ratio of mass ratio 1:5:2, packs in container as described in Figure 1, lights smoldering.After burning, produce cigarette and pass in the collector that 500ml cold water is housed of getting ready in advance, the cigarette that burning 50min produces passes in 500 ml cold water and makes plant resource cigarette water of the present invention, also can be described as cigarette jellyfish liquid.
embodiment 5
Collect deciduous tree one ball plane tree platanus occidentalis; Two ball plane trees platanus acerifolia;sycamore platanus orientaliseach 7kg of withered and yellow fallen leaves, removes various impurity, cleans, and it is dried naturally, according to the ratio of mass ratio 1:10:5, packs in container as described in Figure 1, lights smoldering.After burning, produce cigarette and pass in the collector that 500ml cold water is housed of getting ready in advance, the cigarette that burning 50min produces passes in 500 ml cold water and makes plant resource cigarette water of the present invention, also can be described as cigarette jellyfish liquid.
embodiment 6
Collect deciduous tree one ball plane tree platanus occidentalis; Two ball plane trees platanus acerifolia;sycamore platanus orientaliseach 7kg of withered and yellow fallen leaves, removes various impurity, cleans, and it is dried naturally, according to the ratio of mass ratio 1:10:0.5, packs in container as described in Figure 1, lights smoldering.After burning, produce cigarette and pass in the collector that 500ml cold water is housed of getting ready in advance, the cigarette that burning 50min produces passes in 500 ml cold water and makes plant resource cigarette water of the present invention, also can be described as cigarette jellyfish liquid.
embodiment 7
Collect deciduous tree one ball plane tree platanus occidentalis; Two ball plane trees platanus acerifolia;sycamore platanus orientaliseach 7kg of withered and yellow fallen leaves, removes various impurity, cleans, and it is dried naturally; Fallen leaves are deposited in black room, use ultraviolet lighting 2 days, then use the hot air treatment 5-6h of 75-80 ℃; Fallen leaves after processing are packed in container as described in Figure 1 according to the ratio of mass ratio 1:5:2, light smoldering.After burning, produce cigarette and pass in the collector that 500ml cold water is housed of getting ready in advance, the cigarette that burning 50min produces passes in 500 ml cold water and makes plant resource cigarette water of the present invention, also can be described as cigarette jellyfish liquid.
reference examples 1
According to the method for embodiment 3, prepare cigarette water, different, plant resource used is: yulan ( magnolia denudata), Chinese scholar tree ( sophora japonica), yellow rose ( rosa xanthinalindl) the dry leaf of this three Plants, their mass ratio is 1:1:1.
reference examples 2
According to the method for embodiment 3, prepare cigarette water, different, plant resource used is: cercis ( cercis chinensinbunge), weeping willow ( salix babylonica) ,chinaberry ( melia azedarach) the dry leaf of this three Plants, their mass ratio is 1:1:1.
application examples
Cigarette water for the treatment of the process of red sage root plant is: cigarette jellyfish liquid and water are made into the cigarette aqueous solution according to the volume ratio of 1:500,1:1000 and 1:2000, the pouring water that this cigarette aqueous solution is replaced to red sage root plant, cultivation for red sage root plant, pouring in every 3 days once, consumption 200ml/1000g soil, every 10 days, detect the accumulation of active principle in the red sage root, detect altogether 3 times.
According to the method described above by embodiment 3,4,6,7, the cigarette water of reference examples 1,2 is diluted to 3 kinds of variable concentrations with water respectively, then for red sage root plant, observe red sage root plant strain growth and active principle accumulation, wherein embodiment 3,4,6,7 is as experimental group, reference examples 1,2, as negative control group, adds blank group in addition simultaneously.It is all consistent that each organizes red sage root type of rearing, different: experimental group is respectively with the cigarette water pouring red sage root plant of embodiment 3,4,6,7, and every three days once, each 200ml/1000g soil; Negative control group is respectively with the cigarette water pouring red sage root plant of reference examples 1,2, and every three days once, each 200ml/1000g soil; Blank group is watered red sage root plant with pure water, and every three days once, each 200ml/1000g soil.
The red sage root, has another name called red ginseng, radix salviae miltiorrhizae, and red etc. is dicotyledon Labiatae, is mainly used in treating the heart, brain, vascular disease, obesity and hyperlipidemia.For the evaluation of Salvia miltiorrhiza amount quality, generally with the Tanshinone I wherein containing, the content of joining ketone IIA or Cryptotanshinone, how much weigh at present, so to measure the mode of above-mentioned three kinds of active constituent contents, judge the accumulation of effective component in red sage in this experiment.
Test that within 30 days, respectively to organize afterwards in the red sage root active constituent content as follows:
Figure 240013DEST_PATH_IMAGE001
Figure 957433DEST_PATH_IMAGE002
From the data of above-mentioned table 1-3, can find out, the cigarette water of the embodiment of the present invention 3,4,6,7 is compared the equal effect that can play the accumulation of promotion effective component in red sage with blank, and can find out by the contrast between them, embodiment 7 facilitations are the most remarkable, embodiment 3,4 takes second place, and the facilitation of embodiment 6 is less comparatively speaking.Contrast by cigarette water of the present invention and comparative example cigarette water can find out, the cigarette water of preparation method's gained of the present invention can well promote the accumulation of tanshinone in salvia miltiorrhiza bunge I, ginseng ketone IIA and Cryptotanshinone, and the cigarette water facilitation of comparative example is not clearly.

Claims (10)

1. a plant resource cigarette water, is characterized in that: plant resource is the dry fallen leaves of a ball plane tree, two ball plane trees and these three kinds of arbors of sycamore.
2. plant resource cigarette water according to claim 1, is characterized in that: the mass ratio of a ball plane tree, two ball plane trees and sycamore leaf is 1:1-10:0.5-5.
3. plant resource cigarette water according to claim 1, is characterized in that: the mass ratio of a ball plane tree, two ball plane trees and sycamore leaf is 1:1:1 or 1:5:2.
4. according to the plant resource cigarette water described in any one in claim 1-3, it is characterized in that: described plant resource cigarette water is that the method that the cigarette by 5-7kg plant resource smoldering 40-60min is produced passes in 500 ml cold water makes.
5. the preparation method of the plant resource cigarette water described in any one in a claim 1-3, it is characterized in that comprising the following steps: the withered and yellow fallen leaves of selecting a ball plane tree, two ball plane trees and sycamore, naturally dry, by proportioning, add in closed container, light rear smoldering, the cigarette that smoldering produces, in pipeline passes into the collector that fills water, obtains plant resource cigarette water.
6. preparation method according to claim 5, is characterized in that: after fallen leaves nature dries, first carry out following processing, then carry out smoldering: fallen leaves are deposited in black room, with ultraviolet lighting 1-2 days, then with the thermal current of 75-80 ℃, process 5-6h.
7. preparation method according to claim 6, is characterized in that: described thermal current is hot-air or hot oxygen-enriched air.
8. preparation method according to claim 5, is characterized in that: gained plant resource cigarette water lucifuge low temperature is preserved.
9. a plant resource cigarette water promotes the method that effective component in red sage accumulates, it is characterized in that: the plant resource Yan Shui described in any one in claim 1-3 is mixed according to the volume ratio of 1:500-2000 with water, obtain cigarette water diluent, cultivation process by described cigarette water diluent for red sage root plant, to promote the accumulation of effective component in red sage.
10. method according to claim 9, is characterized in that: every 3 days with the cigarette water diluent pouring red sage root once, consumption is 200ml/1000g soil.
CN201310631035.0A 2013-11-29 2013-11-29 Plant source smoke water and its preparation method and use in promotion of red sage root active-substance accumulation Expired - Fee Related CN103583574B (en)

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Cited By (5)

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CN104782657A (en) * 2015-03-12 2015-07-22 山东省分析测试中心 Plant destructive distillation liquid, its preparation and its application in improvement of content of effective components in hairy root of Salvia miltiorrhiza
CN106754620A (en) * 2017-03-01 2017-05-31 济南大学 A kind of method that utilization plant source cigarette water promotes polysaccharide effective constituents accumulation in the lobate layer bacterium of currant
CN106929464A (en) * 2017-03-01 2017-07-07 济南大学 A kind of method that utilization plant source cigarette water promotes sterol effective constituents accumulation in the lobate layer bacterium of currant
CN109618852A (en) * 2019-01-09 2019-04-16 济南大学 A kind of expanding propagation method of arbuscular mycorrhizal fungi and its application
CN109845449A (en) * 2019-03-21 2019-06-07 山东农业大学 A kind of cigarette water preparation apparatus and method for Seed Germination Test

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104782657A (en) * 2015-03-12 2015-07-22 山东省分析测试中心 Plant destructive distillation liquid, its preparation and its application in improvement of content of effective components in hairy root of Salvia miltiorrhiza
CN104782657B (en) * 2015-03-12 2017-09-29 山东省分析测试中心 A kind of plant dry distillation liquid, its preparation method and its application in Hairy Root Cultures of Salvia miltiorrhiza active constituent content is improved
CN106754620A (en) * 2017-03-01 2017-05-31 济南大学 A kind of method that utilization plant source cigarette water promotes polysaccharide effective constituents accumulation in the lobate layer bacterium of currant
CN106929464A (en) * 2017-03-01 2017-07-07 济南大学 A kind of method that utilization plant source cigarette water promotes sterol effective constituents accumulation in the lobate layer bacterium of currant
CN106754620B (en) * 2017-03-01 2018-09-25 济南大学 A method of promote polysaccharide effective constituents in the lobate layer bacterium of currant to accumulate using plant source cigarette water
CN109618852A (en) * 2019-01-09 2019-04-16 济南大学 A kind of expanding propagation method of arbuscular mycorrhizal fungi and its application
CN109618852B (en) * 2019-01-09 2020-10-16 济南大学 Propagation method of arbuscular mycorrhizal fungi and application thereof
CN109845449A (en) * 2019-03-21 2019-06-07 山东农业大学 A kind of cigarette water preparation apparatus and method for Seed Germination Test

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