CN101317526A - Production and cultivation of glossy ganoderma with tea branch, tea, stem material - Google Patents

Abstract

The invention relates to a production process method for mythic fungus cultivation by tea branches, tea and peduncle-replaced materials. The production process method is characterized in that the proportions are as follows: 33-53% of tea branches, 17-27% of peduncles, 18-48% of wheat bran, 1-2% of brown sugar, 0.5-1% of gypsum and 0.1-0.5% of calcium superphosphate. A plurality of wasted tea branches generated during the cutting process in tea garden, tea and peduncles in the tea making process are sufficiently utilized; furthermore, special inducible factors such as tea polysaccharides, vitamin, trace elements, etc. that are rich in the tea branches and tea peduncles can be utilized to produce the mythic fungus with the 1.5-2.5% of the content of the tea polysaccharides which is higher than that of the current mythic fungus cultivated by the replacing material such as tree sawdust, cotton seed shell, etc. by 24.5-35.8%; meanwhile, the tea can prompt the synthesis of extracellular polysaccharide of mythic fungus, improves the quality and medicinal efficacy of the mythic fungus, cultivates the mythic fungus by the tea branches, tea and peduncle-replaced material, increases new replacing material for the mythic fungus cultivation, prompts the development of Chinese mythic fungus production, and is beneficial to the execution of the Chinese natural forest protection engineering.

Description

Tea branch, tealeaves, stalk are produced cultivating ganoderma for material

Technical field:

The present invention relates to the manufacturing technique method of cultivating ganoderma.

Background technology:

Glossy ganoderma is a kind of traditional nourishing and fit keeping function, the rare Chinese medicine of strengthening the body resistance to consolidate the constitution, and is the medicine edible mushroom of a kind of preciousness in China's medical treasure-house.At present, starting one " glossy ganoderma heat " both at home and abroad.The conventional production methods of glossy ganoderma is the broad-leaved cultivation basswood, and China has implemented since " wildwood protected project ", and the production of glossy ganoderma must turn to adopts other substituting stuff cultivation to take as the leading factor.At present, all can be used for glossy ganoderma from weed tree sawdust, cotton seed hull, ramulus mori, wheat bran and discarded tealeaves and produce, saved a large amount of linden resources, protected virgin forest effectively.

Its original production process method is:

(1). the extract with weed tree sawdust, cotton seed hull, ramulus mori, wheat bran or discarded tealeaves on medium carries out seed selection, domestication and purifying to lucidum strain, and the glossy ganoderma of preparing large-scale production produces bacterium;

(2). weed tree sawdust, cotton seed hull, ramulus mori, wheat bran or discarded tealeaves are carried out pulverization process, and prepare burden spice, pack and sterilization with other batchings as wheat bran, brown sugar, gypsum and mistake calcium etc. according to a certain percentage;

(3). inoculate then, indoor bacterium and go out sesame (indoor wall formula or outdoor earthing) management;

(4). carry out gathering of ganoderma lucidum fruitbody and conidial powder at last.

Studies show that hard hardwood sawdust substituting stuff cultivation is comparatively desirable, and tea tree hard broad-leaved tree just, can be fit to the substituting stuff cultivation glossy ganoderma, have experiment to show simultaneously, tealeaves can promote the synthetic of Ganderma lucidum exocellular polysaccharide (one of main active ingredient of glossy ganoderma), improves quality and the medicinal efficacy of glossy ganoderma.Utilize the special inducible factors such as tea polysaccharide, vitamin and trace element that are rich in tea branch and tealeaves, the stalk, the ganoderma polyoses content of producing is than the high 20%-30% of glossy ganoderma of present weed tree sawdust, the equivalent material cultivation of cotton seed hull, and quality and effect are also better; As employing drink tea later discarded tealeaves and green tea or oolong tea juice or tea powder medium 00, cultivate Ganderma lucidum, its tea polysaccharide of discarded tealeaves, vitamin and the trace elements etc. later of then drinking tea have lost major part, synthetic not having to GL-B induced facilitation, and with green tea or oolong tea juice or the domestication of tea powder medium, cultivate Ganderma lucidum, because having lacked glossy ganoderma produces necessary lignin composition, growth of glossy ganoderma and synthesizing of polysaccharide have been influenced.

We have also carried out update search for this reason, and its pertinent literature has:

Closely related document:

1. inscribe one's name: oak leaf, discarded tealeaves cultivating ganoderma test author: Yan Maohua

Mechanism: Haizhou, Jiangsu Province normal school, Lianyun Harbour periodical name: edible mushroom .2000 (2) .-23-23

General pertinent literature:

1. inscribe one's name: the experimental study author that Ganderma lucidum is tamed in the tea medium: Zou Ligen (1) Wu Yuanfeng (2)

Mechanism: agro-product processing research institute of (1) Hangzhou City Agricultural Science Research Inst., Hangzhou 310024

(2) biological and Chemical Engineering institute of Scientific and Technological Institutes Of Zhejiang, Hangzhou 310012

Periodical name: food research and development .2006,27 (1)-17-20

2. inscribe one's name: low-grade green tea domestication Ganderma lucidum experimental study author: Zou Ligen (1) Ding Yuting (2)

Mechanism: agro-product processing research institute of (1) Hangzhou City Agricultural Science Research Inst., Hangzhou 310024

(2) biological and environmental project institute of Zhejiang Polytechnical University, 310032

Periodical name: Hangzhou agricultural science and technology .2005 (2) .-20-22

3. inscribe one's name: fermented glossy ganoderma tea development preliminary study author: the new hard Zheng Li of woods

Mechanism: academy of agricultural sciences, Fujian Province soil and fertilizer research institute, Foochow

Periodical name: edible mushroom .2000,22 (5) .-41-41

It looks into new conclusion: ... .. do not see that pertinent literature is reported for work and utilize the tealeaves that produced in tea place transformation and a large amount of discarded tea branch that prune to produce and the tea making or tea stalk substituting stuff cultivation glossy ganoderma and demonstration thereof to apply.Above scientific and technological novelty assessment report and conclusion are that annex is attached.

Summary of the invention:

Purpose of the present invention is exactly in order to seek a kind of generation material of comparatively desirable production cultivating ganoderma.

The technical solution used in the present invention is: utilize tea branch, tealeaves, stalk to produce cultivating ganoderma, its spy for material

Levy and be that this proportioning is as follows:

Tea branch 33～53% tealeaves, stalk 17～27% wheat brans 18～48%

Brown sugar 1～2% gypsum 0.5～1% superphosphate 0.1～0.5%

The present invention has the following advantages: a large amount of tealeaves, stalks of being produced in tea branch and the tea making discarded of tea place transformation and pruning generation are fully utilized, utilize the special inducible factors such as tea polysaccharide, vitamin and trace element that are rich in tea branch and tealeaves, the stalk, the ganoderma polyoses content of producing is up to 1.5～2.5%, than the high 24.5-35.8% of glossy ganoderma of present weed tree sawdust, the equivalent material cultivation of cotton seed hull; Have experiment to show simultaneously, tealeaves can promote the synthetic of Ganderma lucidum exocellular polysaccharide (one of main active ingredient of glossy ganoderma), improves quality and the medicinal efficacy of glossy ganoderma; Adopt tea branch, tealeaves, stalk to produce comparatively ideal of cultivating ganoderma,, promote the development of China's glossy ganoderma industry, help the enforcement of China's " wildwood protected project " for cultivation of glossy ganoderma has increased new generation material for material.

Embodiment:

Utilize tea branch, tealeaves, stalk to produce cultivating ganoderma for material, its proportioning is as follows:

Tea branch 33～53% tealeaves, stalk 17～27% wheat brans 18～48%

Brown sugar 1～2% gypsum 0.5～1% superphosphate 0.1～0.5%

Manufacturing technique method:

1. by the extract that on medium, adds tea branch and tealeaves, stalk lucidum strain is carried out seed selection, domestication and purifying, finally prepare the glossy ganoderma that adapts to tea branch and discarded tealeaves, obstructs and produce bacterium for the production of gauge modelling;

2. tea branch and discarded tealeaves, stalk are carried out pulverization process respectively, tea branch bits and tealeaves, stalk bits are mixed to mix thoroughly in 3: 1 ratios be major ingredient, and by following prescription: tea branch bits 33～53%, tealeaves, stalk bits 17～27%, wheat bran 18～48%, brown sugar 1～2%, gypsum 0.5～1%, superphosphate 0.1～0.5% are got the raw materials ready, wheat bran and gypsum are mixed thoroughly with major ingredient earlier, again brown sugar soluble in water and superphosphate are added major ingredient, fully mix thoroughly, composts or fertilisers of cultivating water content 60～70%, pH5.0～6.0.

3. the cultivation stockpile for preparing is pack in time after vexed 1～2 hour, in time sterilization.Two kinds of sterilization branch normal-pressure sterilization and autoclavings: normal-pressure sterilization is 100 ℃ of sterilizations 8～12 hours, gets angry and wants violent, reaches 100 ℃ in 3～5 hours, and autoclaving requires pressure 0.12～0.15Mpa maintenance 2～4 hours.The pocket of sterilizing is to be cooled, and action wants fast during inoculation to inoculating below 35 ℃, and the pocket of sterilizing must be finished inoculation in 24 hours.

4. an indoor bacterium: postvaccinal bacterium bag, in the culturing room that immigration disinfects, layered stacked, sack outwardly, every discharging 5～8 floor heights, stay the passageway between every row, culturing room's temperature is controlled at 20～25 ℃, and humidity is controlled at 60～70%, notes the lucifuge cultivation simultaneously, after 25～35 days, but mycelia just purseful enter out sesame management.

5. but go out two kinds of sesame management locellus interior wall formula and outdoor earthing.

Indoor wall formula goes out sesame: layered stacked, sack outwardly, 4～6 layers of every dischargings, indoor temperature is controlled at 25～30 ℃, humidity is controlled at 75～90%, CO ₂Concentration is controlled at below 0.1%, enough scattered lights be arranged, and light is even, to often take a breath simultaneously, keep air fresh, open the bag back like this and produce fruit body, can gather when fruit body began to launch spore in 30～40 days glossy ganoderma and conidial powder after about 13～17 days.

Outdoor earthing goes out sesame: the ridge-up bed dark 25～30cm that cuts the earth, with 1～2% limewash sterilization furrow face, the bacterium bag carries out surface sterilization with 1～2% liquor potassic permanganate, open an osculum, the setting of bacterium bag is emitted on the ridge-up bed, stay 3～5cm between bag and the bag, earthing is to flat with the bacterium bag then, same temperature is controlled at 25～30 ℃, humidity is controlled at 75～90%, keep good ventilation and uniform scattered light condition, such 13～17 days generation fruit bodys, can gather when fruit body began to launch spore in 30～40 days glossy ganoderma and conidial powder.

Claims (1)

1. a tea branch, tealeaves, stalk are produced cultivating ganoderma for material, it is characterized in that this proportioning is as follows:

Tea branch 33～53% tealeaves, stalk 17～27% wheat brans 18～48%

Brown sugar 1～2% gypsum 0.5～1% superphosphate 0.1～0.5%.