CN103535380A - Crop drought resistant agent as well as application thereof - Google Patents
Crop drought resistant agent as well as application thereof Download PDFInfo
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Abstract
The invention relates to a crop drought resistant agent as well as an application thereof, belonging to the production field of crops. A technical problem to be solved in the invention is to provide a crop drought agent which is safe and good in drought resistance as well as an application thereof. The crop drought resistant agent comprises the following active ingredients in parts by weight: 7-13 parts of compound amino acid, 1-3 parts of betaine, 3-7 parts of fulvic acid, 1-3 parts of organic materials, and proline which accounts for 6%-70% of the total weight of the active ingredients. Meanwhile, the invention further discloses a preparation method and an application of the crop drought resistant agent. Each component of the crop drought resistant agent disclosed by the invention is harmless to a human body and environment, free from complex chemical components, does not cause residue of hormone, does not bring a safety problem, and has advantages of being good in drought resistance, and the like.
Description
Technical field
The present invention relates to crop drought-ressitant agent and application thereof, belong to crops production field.
Background technology
China is a country that drought and water shortage is serious.Freshwater resources total amount is 28000 billion cubic meters, account for 6% of global water resources, be only second to Brazil, Russia and Canadian, occupy the 4th, the world, but only have per capita 2200 cubic metres, be only world average level the 1/4, U.S. 1/5, rank 121 in the world, be the whole world one of 13 countries that water resource is the poorest per capita.Along with the continuous increase of China's population, the fast development of industry and the deterioration of ecotope, China is being faced with more serious water shortage problem, and arid is seriously restricting the raising of China's crop yield.Therefore,, in the face of growing population pressure and the day by day deficient present situation of water resource, study on drought resistance is agriculture important research content always.
Research shows; under drought condition; osmotic adjustment has increase in various degree as materials such as abscisic acids (ABA) as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) etc., endogenous hormones as soluble sugar, inorganic ions, free amino acid, proline etc., some Protective enzyme in plant corpus; directly or indirectly plant is played regulatory role, strengthen the drought resistance of crop.Can therefore, utilize these materials to improve the drought resistance of crop, moisturizing and coercing lower crop yield be the focus that people pay close attention to always.
Drought resistance agent technology also has application aborning at present, more commonly adopts the high molecular polymer with strong absorptive as water-loss reducer, for crop seed soaking or seed dressing, but can only work at crops seedling stage; Also have and adopt antitranspirant, by foliage-spray, make reagent act on pore, to reduce moisture loss, but drought resistance is unstable, also can impact leaf photosynthesis.In recent years, although occurred on market can be for processing seedling stage, the drought resistance agent type that also can spray for the strain phase, this class drought resistance agent mostly is that number of chemical composition is composite to be formed, and complicated component, the safety of crop is needed to be inquired into, and application cost is higher; Meanwhile, also have the drought resistance agent that adopts plant growth regulator to be composited as gibberellin etc., but somatotropin is residual, has caused again new problem.Therefore, finding the effective drought resistance agent of a kind of drought resisting truly, and can not bring safety problem, is that people thirst for the problem solving always.
Summary of the invention
The technical problem to be solved in the present invention is, a kind of safe and crop drought-ressitant agent and application thereof that drought resisting is effective are provided.
Crop drought-ressitant agent of the present invention, its active component is compound amino acid, betain, fulvic acid, organic matter and proline; Wherein, compound amino acid 7～13 weight portions, betain 1～3 weight portion, fulvic acid 3～7 weight portions, organic 1～3 weight portion, proline quality is 6～70% of active component gross mass.Wherein, compound amino acid has polytype, and raw material mainly divides plant type and animal-type, and plant type compound amino acid be take natural high-protein plant as raw material, from plant, extracts, and separated several amino acids exquisiteness forms; Animal-type compound amino acid is that to take various high protein animal bodies be raw material, and after microorganism fermentation, acid and alkali hydrolysis are processed, spraying is dry to be processed.Meanwhile, compound amino acid of the present invention also can adopt art methods preparation and obtain: utilize after the separation such as peat, brown coal, then produce after the fermentation of becoming thoroughly decomposed, rich amino acids, containing compound amino acids such as cystine, tyrosine, serines.In the present invention, compound amino acid preferably adopts commercially available composite aminophenol powder.Wherein, if do not contain proline in compound amino acid, or its proline content is lower than 6% of drought resistance agent active component gross mass, in active component, also needs to add proline, and the quality that makes proline is 6～70% of active component gross mass; If contain proline content in compound amino acid, reach 6～70% of active component gross mass, from cost-saving angle, considered, preferably no longer added proline.
Wherein, organic matter refers to the organic water dissolubility material that contains vital functions, as humus, chitin derivativ, alginic acid, plant polyose, mushroom polysaccharide, vegetable alkaloid etc.
Further, in order to improve the activity of crop drought-ressitant agent, except active component, also contain auxiliary material in described crop drought-ressitant agent, described auxiliary material can adopt conventional auxiliary material, but considering cost and drought resistance agent are active, and described Optimization of Adjuvant is surfactant and water; Described surfactant can adopt conventional kind, the kinds of surfactants that preferred plant extracts, further preferred Tea Saponin, in Tea Saponin saponin content preferably >=30wt%; Described water is preferably distilled water.
Further, described betain is at least one in alkyl betaine, alkyl amido betaine, azochlorosulfonate propyl lycine, alkyl azochlorosulfonate propyl lycine, phosphoric acid fat betain; Organic matter is preferably at least one in humus, chitin derivativ, alginic acid, plant polyose, mushroom polysaccharide, vegetable alkaloid.
Further, as preferred version, the active component of crop drought-ressitant agent of the present invention is comprised of the component of following weight portion: compound amino acid 10 weight portions, proline-4 weight portion, betain 2 weight portions, fulvic acid 5 weight portions, organic 3 weight portions; Wherein, described organic matter is chitin derivativ and lentinan, and described betain is alkyl betaine.
Further, the invention also discloses the preparation method of crop drought-ressitant agent, comprise the steps:
A, according to proportioning, weigh compound amino acid, proline, betain, fulvic acid, organic matter and distilled water;
B, first distilled water is inserted in reactor, open to stir and add successively other components, and stir, obtain.
Further, the invention also discloses the application of crop drought-ressitant agent in crop drought resistance, wherein, described crop is preferably vegetables, and crop drought-ressitant agent of the present invention can be used for common vegetable crop, more preferably tomato, celery, asparagus lettuce or cucumber etc.
Further, in crop drought-ressitant agent, active component concentration is too low can not bring into play its drought resisting effect, and excessive concentration can exert an influence to crop, as preferred version, in use, be diluted with water to betain mass fraction is 0.0006%～0.006% to crop drought-ressitant agent of the present invention; As more preferably scheme, being diluted with water to betain mass fraction is 0.0013%～0.0025%.
Further, in order to improve the drought resisting effect of crop drought-ressitant agent, described application is preferably and is sprayed on crop plant blade face; More preferably plant, during seedling stage, diluted 800-1500 and be doubly sprayed on plant blade face, carried out secondary dispenser after being preferable over 10-15 days, the preferred continuous administration of a season crop 2～3 times, amount of application is 0.04-0.11L/ mu.
Beneficial effect of the present invention:
1, each component of crop drought-ressitant agent of the present invention, all to human body and environmentally friendly, without complicated chemical composition, can not cause the residual of hormone yet, can not bring safety problem.
2, crop drought-ressitant agent of the present invention can well improve crop drought resistance ability, is better than commercially available prod.
3, each component of the present invention is used after mixing, the function of each component has obtained complementary and has coordinated, can make biologic activity and the drought resistance of seedling and plant be organically combined, thereby strengthened the adaptive capacity of crop to water stress environment, avoided these materials unsettled shortcoming of effect when using separately.
4, crop drought-ressitant agent of the present invention can also obviously improve the nutrition condition of crop simultaneously; promote growing and the raising of immunity of crop; strengthen anti-oxidation protection content of material and protective enzyme activity in plant body, improve the ability of crop water suction, moisturizing and removing interior free yl.
Accompanying drawing explanation
Fig. 1 tomato disease index figure;
Fig. 2 tomato state of an illness is recovered figure;
Mda content figure in Fig. 3 tomato.
Embodiment
Crop drought-ressitant agent of the present invention, its active component is compound amino acid, betain, fulvic acid, organic matter and proline; Wherein, compound amino acid 7～13 weight portions, betain 1～3 weight portion, fulvic acid 3～7 weight portions, organic 1～3 weight portion, proline quality is 6～70% of active component gross mass.Wherein, compound amino acid has polytype, and raw material mainly divides plant type and animal-type, and plant type compound amino acid be take natural high-protein plant as raw material, from plant, extracts, and separated several amino acids exquisiteness forms; Animal-type compound amino acid is that to take various high protein animal bodies be raw material, and after microorganism fermentation, acid and alkali hydrolysis are processed, spraying is dry to be processed.Meanwhile, compound amino acid of the present invention also can adopt art methods preparation and obtain: utilize after the separation such as peat, brown coal, then produce after the fermentation of becoming thoroughly decomposed, rich amino acids, containing compound amino acids such as cystine, tyrosine, serines.In the present invention, compound amino acid preferably adopts commercially available composite aminophenol powder.Wherein, if do not contain proline in compound amino acid, or its proline content is lower than 6% of drought resistance agent active component gross mass, in active component, also needs to add proline, and the quality that makes proline is 6～70% of active component gross mass; If contain proline content in compound amino acid, reach 6～70% of active component gross mass, from cost-saving angle, considered, preferably no longer added proline.
Wherein, organic matter refers to the organic water dissolubility material that contains vital functions, as humus, chitin derivativ, alginic acid, plant polyose, mushroom polysaccharide, vegetable alkaloid etc.
Further, in order to improve the activity of crop drought-ressitant agent, except active component, also contain auxiliary material in described crop drought-ressitant agent, described auxiliary material can adopt conventional auxiliary material, but considering cost and drought resistance agent are active, and described Optimization of Adjuvant is surfactant and water; Described surfactant can adopt conventional kind, the kinds of surfactants that preferred plant extracts, further preferred Tea Saponin, in Tea Saponin saponin content preferably >=30wt%; Described water is preferably distilled water.
Further, described betain is at least one in alkyl betaine, alkyl amido betaine, azochlorosulfonate propyl lycine, alkyl azochlorosulfonate propyl lycine, phosphoric acid fat betain; Organic matter is preferably at least one in humus, chitin derivativ, alginic acid, plant polyose, mushroom polysaccharide, vegetable alkaloid.
Further, as preferred version, the active component of crop drought-ressitant agent of the present invention is comprised of the component of following weight portion: compound amino acid 10 weight portions, proline-4 weight portion, betain 2 weight portions, fulvic acid 5 weight portions, organic 3 weight portions; Wherein, described organic matter is chitin derivativ and lentinan, and described betain is alkyl betaine.
Further, the invention also discloses the preparation method of crop drought-ressitant agent, comprise the steps:
A, according to proportioning, weigh compound amino acid, proline, betain, fulvic acid, organic matter and distilled water;
B, first distilled water is inserted in reactor, open to stir and add successively other components, and stir, obtain.
Further, the invention also discloses the application of crop drought-ressitant agent in crop drought resistance, wherein, described crop is preferably vegetables, and crop drought-ressitant agent of the present invention can be used for common vegetable crop, more preferably tomato, celery, asparagus lettuce or cucumber etc.
Further, in crop drought-ressitant agent, active component concentration is too low can not bring into play its drought resisting effect, and excessive concentration can exert an influence to crop, as preferred version, in use, be diluted with water to betain mass fraction is 0.0006%～0.006% to crop drought-ressitant agent of the present invention; As more preferably scheme, being diluted with water to betain mass fraction is 0.0013%～0.0025%.
Further, in order to improve the drought resisting effect of crop drought-ressitant agent, described application is preferably and is sprayed on crop plant blade face; More preferably plant, during seedling stage, diluted 800-1500 and be doubly sprayed on plant blade face, carried out secondary dispenser after being preferable over 10-15 days, the preferred continuous administration of a season crop 2～3 times, amount of application is 0.04-0.11L/ mu.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
The preparation of embodiment 1 crop drought-ressitant agent of the present invention
A, according to each proportioning in table 1, take each component;
B, first distilled water is inserted in reactor, open to stir and add successively other components, and stir, obtain.
Table 1 is respectively organized drought resistance agent component and proportioning thereof
Table 2 compound amino acid supply producer and raw batch number
Drought resistance experiment-the tomato of embodiment 2 drought resistance agents of the present invention
1, experiment material:
Experiment crop: tomato seedling, 13 strain * 13 group;
Cultivation matrix: the peat composed of rotten mosses, vermiculite
Experiment reagent: KH-01, KH-02
Contrast agents: Mei Jiafu (manufacturer: Italian tile glug sieve joint-stock company, agent: Jiangmen city Jie Shi agricultural science and technology Co., Ltd, main component is: polypeptide >=380 grams per liter, amino acid >=100 grams per liter, organic nitrogen >=60 grams per liter, (chelating state) iron+zinc+manganese >=20 grams per liter), the date of manufacture: 20110110)
2, experiment material:
Green house.Each tests the mid portion that community should be placed on greenhouse.The condition uniformities such as the soil texture of all experiments community, pH value, the content of organic matter, fertilizing management situation, planting density, and it is actual to meet local agricultural production.
3, experimental technique and design
3.1 agent treated
Reagent proportioning is in Table 1, and agent treated situation is in Table 3.
Table 3 experiment reagent is processed
| Numbering | Experiment is processed | Extension rate | Amount of application | Spraying time |
| 1-1 | KH-01 | 1000 | 100ml | Before arid |
| 1-2 | KH-01 | 1000 | 100ml | After arid |
| 2-1 | KH-01 | 500 | 100ml | Before arid |
| 2-2 | KH-01 | 500 | 100ml | After arid |
| 3-1 | KH-02 | 1000 | 100ml | Before arid |
| 3-2 | KH-02 | 1000 | 100ml | After arid |
| 4-1 | KH-02 | 500 | 100ml | Before arid |
| 4-2 | KH-02 | 500 | 100ml | After arid |
| 5-1 | Mei Jiafu | 1000 | 100ml | Before arid |
| 5-2 | Mei Jiafu | 1000 | 100ml | After arid |
| 6-1 | CK1 | Clear water | 100ml | Before arid |
| 6-2 | CK2 | Clear water | 100ml | After arid |
| 7 | CK3 | Clear water | 100ml | Without arid, process |
3.2 experimental design
8 processing (5 contrasts) are established in this test, and every processing repeats for 1 time, amounts to 13Ge community.Each community randomized arrangement, each community 13 basin tomato, tomato experiment Quarter Design table is in Table 4.
Table 4 tomato experiment Quarter Design table
4 investigation, record, measuring method
4.1 meteorologies and soil information
Record intensity of illumination and the temperature (daily mean temperature, the highest and minimum temperature, with a ℃ expression) of experimental session.Data should or be preferably in sample plot record from nearest meteorological station.
Record the data such as soil types, soil fertility, soil moisture content, weeds coverage condition.
4.2, investigation method
Arid 2(Extreme drought in experimentation respectively), carry out 2 investigation (recovering after Extreme drought) two periods of watering.
In the evaluation of tomato seedling Identification of Drought not unified standard in the situation that, this experimental basis tomato plant has been formulated respectively damage caused by a drought and the grade scale that recovers of Extreme drought in seedling stage at Extreme drought and the Symptoms that waters after recovering.
Seedling stage damage caused by a drought grade scale:
0 grade---seedling is normal, without any symptom;
1 grade---young leaves is dim, and siphonal lobe is obviously wilted;
3 grades---young leaves is obviously wilted, and siphonal lobe is seriously wilted, and stem is upright;
5 grades---blade is seriously wilted, stem lodging or half lodging;
7 grades---the plant dehydration of seriously wilting, stem lodging.
Wherein 5 grades, 7 grades is Extreme drought symptom.
Extreme drought recovers grade scale:
0 grade---seedling recovers normally completely, without any arid injury symptom;
1 grade---seedling recovers normally substantially, and bottom 1-2 sheet leaf shows obvious wilting;
3 grades---cane is upright, and growing point has vigor, and serious wilting of blade is difficult to recover normal, and plant, is subject to grievous injury;
5 grades---the plant death of seriously wilting, irrecoverable.
Can survey auxiliary characteristics: leaf area changes, rhizome ratio.
4.3 measuring method
In above-mentioned 2 investigation, gather blade respectively, measure blade malonaldehyde accumulation content.
Arid and plant membrane injury close relation.Arid can cause the damage of cell membrane system, can cause plant cell dead when serious.Superoxide dismutase (SOD) is the main protective enzyme of film lipid peroxidation system of defense, and it can catalytic activity oxygen generation disproportionated reaction produce nontoxic molecular oxygen and hydrogen peroxide, thereby avoids plant to sustain an injury.Catalase (CAT) and peroxidase (POD) are all the protective enzymes in film lipid peroxidation system of defense, can remove the H in physiological system
2o
2.Malonaldehyde (MDA) is one of primary product of film lipid peroxidation, MDA content under drought stress raises with the increase of arid intensity, can in inducing cell, there is Solute accumulation in plant, by osmotic adjustment, reduce the flow of water, thereby while guaranteeing to organize the flow of water to decline, cell turgor is maintained, that is, mda content is lower, and its drought resistance is better.The osmotic adjustment of plant mainly realizes by the accumulation of compatibility solute.Thereby can infer crop drought resistance sexual state by measuring mda content.
5 Analysis of conclusions
5.1 experimental result
In the arid 2(Extreme drought in experimentation) time investigate original disease index, shown in disease index investigation raw data table 5, original disease index figure is as shown in Figure 1.
Table 5 tomato disease index
| |
0 grade | 1 grade | 3 |
5 |
7 grades | Disease index/% |
| 6-2 | ? | 1 | 8 | ? | 4 | 40.77 |
| 6-1 | ? | 2 | 8 | 2 | 1 | 33.08 |
| 5-2 | ? | 2 | 10 | ? | 1 | 30.00 |
| 5-1 | ? | 2 | 11 | ? | ? | 26.92 |
| 4-2 | ? | 2 | 11 | ? | ? | 26.92 |
| 3-2 | ? | 4 | 8 | 1 | ? | 25.38 |
| 2-2 | ? | 5 | 7 | 1 | ? | 23.85 |
| 4-1 | ? | 4 | 9 | ? | ? | 23.85 |
| 3-1 | ? | 6 | 7 | ? | ? | 20.77 |
| 2-1 | ? | 7 | 6 | ? | ? | 19.23 |
| 1-2 | ? | 7 | 6 | ? | ? | 19.23 |
| 1-1 | ? | 8 | 5 | ? | ? | 17.69 |
| 7 | 13 | ? | ? | ? | ? | 0 |
Disease index (R) is calculated as follows:
R=（N
0*0+N
1*0.1+N
3*0.3+N
5*0.5+N
7*0.7）/n*100%
Wherein, N
0, N
1, N
3be respectively grade and be the tomato seedling number of 0 grade, 1 grade, 3 grades etc., n is total seedling number.
In the arid 2(Extreme drought in experimentation) after water, and after 2d, observe recovery situation and measure mda content, after 2d, disease index is in Table 6, the state of an illness is recovered figure as shown in Figure 2; Mda content is in Table 7, and mda content figure as shown in Figure 3.
Table 6 tomato state of an illness index of recovery
| |
0 grade | 1 grade | 3 |
5 grades | Disease index/% |
| 6-2 | ? | ? | 3 | 7 | 37.61 |
| 6-1 | ? | 1 | 5 | 3 | 31.62 |
| 5-2 | ? | 4 | 5 | 2 | 28.21 |
| 5-1 | ? | ? | 10 | ? | 25.64 |
| 4-2 | ? | ? | 10 | ? | 25.64 |
| 3-2 | ? | 1 | 7 | 1 | 24.79 |
| 2-2 | ? | 2 | 8 | ? | 22.22 |
| 4-1 | ? | 3 | 7 | ? | 20.51 |
| 3-1 | ? | 5 | 5 | ? | 17.09 |
| 2-1 | ? | 6 | 4 | ? | 15.38 |
| 1-2 | ? | 6 | 4 | ? | 15.38 |
| 1-1 | ? | 7 | 3 | ? | 13.68 |
| 7 | 13 | ? | ? | ? | 0 |
Mda content in table 7 tomato
| Community | Mda content | Appearance character |
| 6-2 | 0.06390 | Wilting seedling |
| 6-1 | 0.06130 | Wilting seedling |
| 5-2 | 0.05124 | Wilting seedling |
| 5-1 | 0.04929 | Wilting seedling |
| 4-2 | 0.04600 | Wilting seedling |
| 3-2 | 0.03857 | Wilting seedling |
| 2-2 | 0.03804 | Wilting seedling |
| 4-1 | 0.03568 | Wilting seedling |
| 3-1 | 0.03530 | Wilting seedling |
| 2-1 | 0.03455 | Wilting seedling |
| 1-2 | 0.03235 | Wilting seedling |
| 1-1 | 0.03086 | |
| 7 | 0.02699 | Plant is normal |
According to Fig. 1 and table 5,6-2(CK2, waters after drought) and 6-1(CK1, before drought, water) to compare, 6-1 disease index is lower, so waters than the better effects if of watering after drought before can finding out drought, and meanwhile, each experimental group has also been verified this conclusion; Meanwhile, rank the first be 1-1(KH-01-1000 *, drought before spray medicine).
According to Fig. 2 and table 6, analyze, 1-1 disease index is minimum, is also that effect is best, and its recovery situation is best.
According to Fig. 3 and table 7, analyze, that mda content is the highest is 6-2(CK1, after drought, waters), that is to say the maximum of coercing that the tomato seedling of watering after drought is subject to, this result and the first two result are identical.
To sum up, compare with contrast agents, 1-1 all shows obvious otherness, and successful excellence is in competing product, and KH-01-1000 * effect is better.
5.2 Analysis of conclusion
This tests the tomato seedling disease index that waters before drought and mda content all lower than watering after drought, this all shows except uniformity accurately in various medicaments, simultaneously, before KH-01 reagent drought, water consistent with the performance of watering after drought all in same processing and effect is best, and drought resistance agent effect of the present invention is better than contrast agents, further verified that reagent drought resisting of the present invention has good drought resisting effect.
Drought resistance experiment-the asparagus lettuce of embodiment 3 drought resistance agents of the present invention
1 experiment material:
Experiment crop: asparagus lettuce, 9 strain * 10 group;
Experiment reagent: KH-07, KH-08
Contrast agents: western Mg(A-Micsur Mg， Spain Probelte company produces, batch number: FECHA06/06/2011LOTE11060134)
2 test sites
Green house, indoor temperature maintains 12-28 ℃.Each experimental plot should be placed on the mid portion in greenhouse.The condition uniformities such as the soil texture of all experimental plots, pH value, the content of organic matter, fertilizing management situation, planting density, and it is actual to meet local agricultural production.
3 test methods and design
3.1 reagent names and concentration
Reagent proportioning is in Table 1, and the concentration of reagent is in Table 8.
Table 8 reagent and concentration are processed
| Numbering | Reagent | Extension rate | Amount of application | Community area |
| 1 | KH-07 | 800 | 80ml | Each community 9 |
| 2 | KH-07 | 1500 | 80ml | Each community 9 basin |
| 3 | KH-08 | 800 | 80ml | Each community 9 basin |
| 4 | KH-08 | 1500 | 80ml | Each community 9 |
| 5 | Western Mg | 800 | 80ml | Each community 9 basin |
| 6 | Western Mg | 1500 | 80ml | Each community 9 |
| 7 | CK | Normally water | 80ml | Each community 9 basin |
| 8 | CK | Arid | 80ml | Each community 9 basin |
3.2 experiment Quarter Designs
8 processing (2 contrasts) are established in this test, and every processing repeats for 1 time, amounts to 10Ge community.Each community randomized arrangement,
Each community 9 basin asparagus lettuce, experimental plot design table is in Table 9.
Community, table 9 asparagus lettuce seedbed distribution table
4 test methods
4.1 asparagus lettuces are processed and spray medicine
Asparagus lettuce size uniformity is chosen in test, and carries out dispenser without bad upgrowth situation.
4.2 investigation method
Arid 2(Extreme drought in experimentation respectively), carry out 2 investigation (recovering after Extreme drought) two periods of watering.
At asparagus lettuce drought resistance of seedling characterization and evaluation not unified standard in the situation that, this test basis asparagus lettuce plant has been formulated respectively damage caused by a drought and the grade scale that recovers of Extreme drought in seedling stage at Extreme drought and the Symptoms that waters after recovering, and grade scale is with embodiment 1.
4.3 measuring method
In above-mentioned 2 investigation, gather blade respectively, measure blade malonaldehyde accumulation content.
Adopt the processing method of Extreme drought to carry out Evaluation of drought resistance to it, to determine that tomato seedling is used the change of drought resistance after preparation.
5 test results and analysis
5.1 result of the test
Data survey is in Table 10:
The impact of the different reagent of table 10 on asparagus lettuce Drought resistance rank
By table 10 result, shown, and contrast CK(arid) to compare, different reagent different disposal have remarkable result to asparagus lettuce drought-resistant ability.Wherein, KH-07-1500, KH-08-800, KH-08-1500 are the strongest to asparagus lettuce drought-resistant ability, and drought symptom is partially light.
After 7d, test mda content in different disposal group asparagus lettuce, every group of random three strain asparagus lettuces of selecting, mda content the results are shown in Table 11.
Mda content in table 11 different disposal group asparagus lettuce
5.3 Analysis of conclusion
Table 10 result shows, and contrasts CK(arid) to compare, different reagent different disposal have remarkable result to asparagus lettuce drought-resistant ability.Wherein, KH-07-1500, KH-08-800, KH-08-1500 are the strongest to asparagus lettuce drought-resistant ability, and drought symptom is partially light.
Table 11 result shows, with contrast CK(arid) compare, in different its asparagus lettuces of agent treated, mda content all has reduction, wherein, KH-07, KH-08 process to reduce significantly, especially KH-07-1500 *, KH-08-800 *, KH-08-1500 *, with contrast ratio, all have significant difference, effect is remarkable.
6, experiment conclusion
Comprehensive different reagent and the impact of concentration on asparagus lettuce Drought resistance rank, and the impact of different reagent on mda content in asparagus lettuce, drought resistance agent formula KH-08 is under 800 and 1500 times of effects, to the drought resisting effect of asparagus lettuce compared with the control, effect is the most obvious, that is, drought resistance agent of the present invention has good drought resistance, is better than commercially available prod.
Drought resistance experiment-the celery of embodiment 4 drought resistance agents of the present invention
1 test material
Trial crops: celery 90 strains, 9 strain * 10 group.
1, experiment material:
Experiment crop: celery
Experiment reagent: KH-07, KH-08
Contrast agents: claim western Mg(A-Micsur Mg， Spain Probelte company to produce, batch number: FECHA06/06/2011LOTE11060134)
2 test sites
Green house, indoor temperature maintains 12-28 ℃.Each experimental plot should be placed on the mid portion in greenhouse.The condition uniformities such as the soil texture of all experimental plots, pH value, the content of organic matter, fertilizing management situation, planting density, and it is actual to meet local agricultural production.
3 experimental scheme
3.1 reagent names and concentration
Reagent proportioning is in Table 1, and the concentration of reagent is in Table 12.
Table 12 reagent and concentration are processed
| Numbering | Reagent | Extension rate | Amount of application | Community area |
| 1 | KH-07 | 800 | 80ml | Each community 9 |
| 2 | KH-07 | 1500 | 80ml | Each community 9 basin |
| 3 | KH-08 | 800 | 80ml | Each community 9 basin |
| 4 | KH-08 | 1500 | 80ml | Each community 9 |
| 5 | Western Mg | 800 | 80ml | Each community 9 basin |
| 6 | Western Mg | 1500 | 80ml | Each community 9 |
| 7 | CK | Normally water | 80ml | Each community 9 basin |
| 8 | CK | Arid | 80ml | Each community 9 basin |
3.2 Quarter Design
8 processing (2 contrasts) are established in this test, and every processing repeats for 1 time, amounts to 10Ge community.Each community randomized arrangement, each community 9 basin celery, experiment community distribution table is in Table 13.
Community, table 13 seedbed distribution table
4 test methods
4.1 celeries are processed and spray medicine
Celery size uniformity is chosen in test, and carries out dispenser without bad upgrowth situation.
4.2 investigation method
Arid 2(Extreme drought in experimentation respectively), carry out 2 investigation (recovering after Extreme drought) two periods of watering.
In the evaluation of celery Identification of Drought in seedling stage not unified standard in the situation that, this test basis celery plant has been formulated respectively damage caused by a drought and the grade scale that recovers of Extreme drought in seedling stage at Extreme drought and the Symptoms that waters after recovering, and grade scale is with embodiment 1.
4.3 measuring method
If investigation method can not be successful, detect the variation of inclusion.In above-mentioned 2 investigation, gather blade respectively, measure blade malonaldehyde accumulation content.
Adopt the processing method of Extreme drought to carry out Evaluation of drought resistance to it, to determine that celery is used the change of drought resistance after preparation seedling stage.
5 test results and analysis
5.1 result of the test
Respectively at the laggard line number of dispenser 5d according to investigations, data survey is in Table 14 in this test:
The impact of the different reagent of table 14 on celery Drought resistance rank
Table 14 result shows, and contrasts CK(arid) to compare, different reagent different disposal have remarkable result to celery drought-resistant ability.
After 5d, test mda content in different disposal group celery, every group of random three strain celeries of selecting, mda content the results are shown in Table 15.
The impact of table 15 different disposal on celery mda content
Table 15 result shows, and contrasts CK(arid) compare, different reagent concentrations are processed mda content in its celery and are all had remarkable reduction, wherein, KH-08-800 * and KH-08-1500 * processing significantly reduce, there is utmost point significant difference, effect is remarkable.
5.2 Analysis of conclusion
Table 14 result shows, and contrasts CK(arid) to compare, different reagent different disposal have remarkable result to celery drought-resistant ability.Wherein, KH-08 is the strongest to celery drought-resistant ability, and drought symptom is partially light.
Table 15 result shows, and contrasts CK(arid) to compare, in its celery of different agent treated, mda content all has reduction, and wherein, KH-08 processes and reduces significantly, with contrast ratio, all has utmost point significant difference, and effect is remarkable.
6, conclusion (of pressure testing)
Comprehensive different reagent and the impact of concentration on celery Drought resistance rank, and the impact of different reagent on mda content in celery, to the drought resisting effect of celery compared with the control, effect is the most obvious for drought resistance agent formula KH-08; Meanwhile, the drought resisting effect of drought resistance agent of the present invention all shows excellence, and drought resistance is better than commercially available prod.
Drought resistance experiment-the cucumber of embodiment 5 drought resistance agents of the present invention
1 test material
Trial crops: cucumber 90 strains, 9 strain * 10 group.
1, experiment material:
Experiment crop: cucumber
Experiment reagent: KH-03, KH-04
Contrast agents: western Mg(A-Micsur Mg， Spain Probelte company produces, batch number: FECHA06/06/2011LOTE11060134)
2 test sites
Green house, indoor temperature maintains 12-28 ℃.Each experimental plot should be placed on the mid portion in greenhouse.The condition uniformities such as the soil texture of all experimental plots, pH value, the content of organic matter, fertilizing management situation, planting density, and it is actual to meet local agricultural production.
3 experimental scheme
3.1 reagent names and concentration
Reagent proportioning is in Table 1, and the concentration of reagent is in Table 16.
Table 16 reagent and concentration are processed
| Numbering | Reagent | Extension rate | Amount of application | Community area |
| 1 | KH-03 | 800 | 90ml | Each community 9 |
| 2 | KH-03 | 1500 | 90ml | Each community 9 basin |
| 3 | KH-04 | 800 | 90ml | Each community 9 basin |
| 4 | KH-04 | 1500 | 90ml | Each community 9 |
| 5 | Western Mg | 800 | 90ml | Each community 9 basin |
| 6 | Western Mg | 1500 | 90ml | Each community 9 |
| 7 | CK | Normally water | 90ml | Each community 9 basin |
| 8 | CK | Arid | 90ml | Each community 9 basin |
3.2 Quarter Design
8 processing (2 contrasts) are established in this test, and every processing repeats for 1 time, amounts to 10Ge community.Each community randomized arrangement, each community 9 basin cucumber, experiment community distribution table is in Table 17.
Community, table 17 seedbed distribution table
4 test methods
4.1 cucumber are processed and spray medicine
Cucumber size uniformity is chosen in test, and carries out dispenser without bad upgrowth situation.
4.2 investigation method
Arid 2(Extreme drought in experimentation respectively), carry out 2 investigation (recovering after Extreme drought) two periods of watering.
In the evaluation of cucumber at seedling stage Identification of Drought not unified standard in the situation that, this test basis cucumber plant has been formulated respectively damage caused by a drought and the grade scale that recovers of Extreme drought in seedling stage at Extreme drought and the Symptoms that waters after recovering, and grade scale is with embodiment 1.
4.3 measuring method
In above-mentioned 2 investigation, gather blade respectively, measure blade malonaldehyde accumulation content.
Adopt the processing method of Extreme drought to carry out Evaluation of drought resistance to it, to determine that celery is used the change of drought resistance after preparation seedling stage.
5 test results and analysis
5.1 result of the test
Respectively at the laggard line number of dispenser 5d according to investigations, data survey is in Table 18 in this test:
The impact of the different reagent of table 18 on cucumber Drought resistance rank
Table 18 result shows, and contrasts CK(arid) to compare, different reagent different disposal have remarkable result to cucumber drought-resistant ability.
After 5d, test mda content in different disposal group cucumber, every group of random selection three strain cucumber, mda content the results are shown in Table 19.
The impact of table 19 different disposal on cucumber mda content
Table 19 result shows, and contrasts CK(arid) compare, different reagent concentrations are processed mda content in its cucumber and are all had remarkable reduction, wherein, KH-04-800 * and KH-04-1500 * processing significantly reduce, there is utmost point significant difference, effect is remarkable.
5.2 Analysis of conclusion
Table 18 result shows, and contrasts CK(arid) to compare, different reagent different disposal have remarkable result to cucumber drought-resistant ability.Wherein, KH-04 is the strongest to cucumber drought-resistant ability, and drought symptom is partially light.
Table 19 result shows, and contrasts CK(arid) to compare, in its cucumber of different agent treated, mda content all has reduction, and wherein, KH-04 processes and reduces significantly, with contrast ratio, all has utmost point significant difference, and effect is remarkable.
6, conclusion (of pressure testing)
Comprehensive different reagent and the impact of concentration on cucumber Drought resistance rank, and the impact of different reagent on mda content in cucumber, to the drought resisting effect of cucumber compared with the control, effect is the most obvious for drought resistance agent formula KH-04; Meanwhile, the drought resisting effect of drought resistance agent of the present invention all shows excellence, and drought resistance is better than commercially available prod.
Drought resistance experiment-other Vegetables of embodiment 6 drought resistance agents of the present invention
Adopt KH-05, KH-06, and adopt the method for embodiment 5 also to carry out drought resistance experiment to Vegetables such as romaine lettuce, capsicums, experimental result all shows, the drought resistance of crop drought-ressitant agent of the present invention is excellent, is better than commercially available prod.
Claims (10)
1. crop drought-ressitant agent, is characterized in that: its active component is compound amino acid, betain, fulvic acid, organic matter and proline; Wherein, compound amino acid 7～13 weight portions, betain 1～3 weight portion, fulvic acid 3～7 weight portions, organic 1～3 weight portion, proline quality is 6～70% of active component gross mass.
2. crop drought-ressitant agent according to claim 1, is characterized in that: except active component, also contain auxiliary material in described crop drought-ressitant agent, described auxiliary material is surfactant and water.
3. crop drought-ressitant agent according to claim 1 and 2, is characterized in that: described surfactant is Tea Saponin, saponin >=30wt% in described Tea Saponin.
4. according to the crop drought-ressitant agent described in claim 1～3 any one, it is characterized in that: described betain is at least one in alkyl betaine, alkyl amido betaine, azochlorosulfonate propyl lycine, alkyl azochlorosulfonate propyl lycine, phosphoric acid fat betain; Described organic matter is at least one in humus, chitin derivativ, alginic acid, plant polyose, mushroom polysaccharide, vegetable alkaloid.
5. crop drought-ressitant agent according to claim 4, is characterized in that: its active component is comprised of the component of following weight portion: compound amino acid 10 weight portions, proline-4 weight portion, betain 2 weight portions, fulvic acid 5 weight portions, organic 3 weight portions; Wherein, described organic matter is chitin derivativ and lentinan; Described betain is alkyl betaine.
6. the application of claim 1～5 any one crop drought-ressitant agent in crop drought resistance.
7. the application of crop drought-ressitant agent according to claim 6 in crop drought resistance, is characterized in that: described crop is vegetables; Be preferably tomato, celery, asparagus lettuce or cucumber.
8. according to the application of the crop drought-ressitant agent described in claim 6 or 7, it is characterized in that: during application, being diluted with water to betain mass fraction is 0.0006%～0.006%.
9. the application of crop drought-ressitant agent according to claim 8, is characterized in that: during application, being diluted with water to betain mass fraction is 0.0013%～0.0025%.
10. according to the application of the crop drought-ressitant agent described in claim 6～9 any one, it is characterized in that: described in be applied as and be sprayed on crop plant blade face.
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| CN109329305A (en) * | 2018-09-29 | 2019-02-15 | 江苏农林职业技术学院 | Tillandsia copper ion drought resistance agent and the preparation method and application thereof |
| CN109329301A (en) * | 2018-09-29 | 2019-02-15 | 江苏农林职业技术学院 | Tillandsia drought resistance agent and its preparation method and application |
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