CN108456055A - A kind of arabidopsis drought resistance agent, preparation method and application method - Google Patents
A kind of arabidopsis drought resistance agent, preparation method and application method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention belongs to plant drought-resisting agent technical fields, and in particular to a kind of arabidopsis drought resistance agent, preparation method and application method.The arabidopsis drought resistance agent is made of the raw material components of following parts by weight：50~100 parts of fermented maize slurry, 30~45 parts of fermented soybean milk, 5~15 parts of potassium fulvate, 1~5 part of gibberellin, 1~3 part of soluble starch；The fermented maize slurry is to mix niblet, sterile water with bacillus subtilis microbial agent, Bifidobacterium microbial inoculum, is fermented, and sterilizing adds water to stir, the filtrate collected after filtering；The fermented soybean milk is to mix soybean, sterile water with bacillus subtilis microbial agent, is fermented, and sterilizing adds water to stir, the filtrate collected after filtering.Drought resistance agent provided by the invention can improve tolerance of the arabidopsis seed under drought condition, and seed germination rate, survival rate of seedling and leaf relative water content increase.
Description
Technical field
The invention belongs to plant drought-resisting agent technical fields, and in particular to a kind of arabidopsis drought resistance agent, preparation method and use
Method.
Background technology
Arabidopsis and rape, radish, cabbage etc. are all crucifer, widely distributed as a kind of herbaceous plant
It is equal in provinces such as the Inner Mongol in China, Xinjiang, Shaanxi, Gansu, Tibet, Shandong, Jiangsu, Anhui in Eurasia and the African northwestward
There is growth.Nearly one over 100 years, and with flourishing for biology and classical genetics, arabidopsis is since genome is small, growth week
The advantages that phase is short, plant is small, solid more is known as " drosophila in plant " as the model plant of plant research.
China's water resource is seriously deficient, increases with the development of economy with the size of population, the shape of China's Proposals of Water Resources
Gesture is more acute, affected by a natural, all different degrees of threat that undergo soil moisture stress in majority of plants.
Therefore the drought-resistant ability for improving crop is of great significance for the sustainable development and grain security that ensure China's agricultural economy.
Arabidopsis for exploitation crops phytochemistry aqua for drought resistance, carries out heredity of drought resistance genetic analysis, gene cloning tool as model plant
There is important economic implications.
Phytochemistry aqua for drought resistance technology commonly used in the prior art is primarily referred to as reaching the holding soil water by using chemical substance
Divide, the technology, such as antitranspirant, water-absorbing resins etc. of moisture loss caused by reduction Crop transpirstion, but these compounds
Safety and degradability need to be further increased.There are also drought resistance agents to use gibberellin as conditioning agent, but gibberellin
Main function be promote crop growth, substantially do not have water imbibition.For example Chinese patent CN102138560B is disclosed
A kind of organic nutritional drought-resistance agent and preparation method thereof, in the drought resistance agent containing arginine, cystine, aspartic acid,
The multiple compounds component such as salicylic acid, dimethyl sulfoxide (DMSO), but the dimethyl sulfoxide (DMSO) etc. in the formula belongs to chemical organic reagent,
Its safety and degradability need to be further increased.Therefore, it is particularly important to develop a kind of safe drought resistance agent.
Invention content
The object of the present invention is to provide a kind of arabidopsis drought resistance agent, preparation method and application method, the drought resistance agent water imbibitions
By force, safe, and the growth of arabidopsis can be adjusted, solve drought resistance agent safety in the prior art and degradability compared with
Difference, or itself do not have the problem of water imbibition.
A kind of arabidopsis drought resistance agent provided by the invention, is made of the raw material components of following parts by weight：Fermented maize slurry 50
~100 parts, 30~45 parts of fermented soybean milk, 5~15 parts of potassium fulvate, 1~5 part of gibberellin, 1~3 part of soluble starch；
The fermented maize slurry is the niblet that will be sterilized, sterile water, bacillus subtilis microbial agent and Bifidobacterium microbial inoculum
Mixing is fermented, and sterilizing adds water to stir, the filtrate collected after filtering；
The fermented soybean milk is that the soybean that will be sterilized, sterile water are mixed with bacillus subtilis microbial agent, is fermented, and sterilizing adds
Water stirs, the filtrate collected after filtering.
Preferably, above-mentioned arabidopsis drought resistance agent, is made of the raw material components of following parts by weight：Fermented maize starches 60 parts, hair
40 parts of ferment soya-bean milk, 10 parts of potassium fulvate, 3 parts of gibberellin, 2 parts of soluble starch.
The present invention also provides a kind of preparation methods of above-mentioned arabidopsis drought resistance agent, include the following steps：
S1 prepares fermented maize slurry
By the niblet of disinfection, sterile water, bacillus subtilis microbial agent and Bifidobacterium microbial inoculum according to 1000:10~15:
3:5 weight ratio mixing, 20-30 DEG C of fermentation 3d, sterilizing are ground into corn steep liquor, are then added and are equivalent to 50 times of corn steep liquor
The water of quality stirs, filtering, and the filtrate of collection is fermented maize slurry；
S2 prepares fermented soybean milk
By the soybean of disinfection, sterile water and bacillus subtilis microbial agent according to 1000:10~15:3 weight ratio is mixed
It closes, 20-30 DEG C of fermentation 5d, sterilizing is ground into soya-bean milk, the water for being equivalent to 50 times of quality of soya-bean milk is then added, stir, filter, receives
The filtrate of collection is fermented soybean milk；
S3 prepares drought resistance agent
Each component is weighed by following parts by weight：50~100 parts of fermented maize slurry, 30~45 parts of fermented soybean milk, potassium fulvate 5
~15 parts, 1~5 part of gibberellin, 1~3 part of soluble starch；The each component mixing that will be weighed, 5~20min is boiled in stirring, cold
But, it filters, the filtrate of collection is to obtain arabidopsis drought resistance agent.
Preferably, in the preparation method of above-mentioned arabidopsis drought resistance agent, after the niblet of disinfection is impregnated 3~5h in water,
It drains away the water, is then used further to prepare fermented maize slurry；It after the soybean of disinfection is impregnated 4~6h in water, drains away the water, then
It is used further to prepare fermented soybean milk.
Preferably, the condition to sterilize in the preparation method of above-mentioned arabidopsis drought resistance agent, S1 and S2 is boiling sterilization 20min.
Preferably, the time stirred in the preparation method of above-mentioned arabidopsis drought resistance agent, S1 and S2 is 30min, speed of agitator
For 200r/min.
The present invention also provides a kind of application methods of above-mentioned arabidopsis drought resistance agent, include the following steps：By arabidopsis kind
Son is soaked in 18h in the arabidopsis drought resistance agent, then takes out the seed after impregnating, is collected simultaneously the drought resisting for having impregnated seed
Agent；The drought resistance agent of seed will have been impregnated with arabidopsis culture soil matrix according to 1:500 parts by weight are mixed thoroughly, and culture medium is obtained
Matter, the seed after sowing immersion.
Compared with prior art, arabidopsis drought resistance agent provided by the invention, has the advantages that：
(1) drought resistance agent provided by the invention prepares raw material and uses native compound, and safe, which can improve
Tolerance of the arabidopsis seed under drought condition, seed germination rate, survival rate of seedling and leaf relative water content are carried
It is high.The use of fermented maize slurry, fermented soybean milk can improve malonaldehyde in blade, proline, Chlorophyll SPAD Reading, improve quasi-
The drought-resistant ability of southern mustard, in addition fermented maize slurry and fermented soybean milk also provide nitrogen and trace element for the growth of arabidopsis；
On the one hand potassium fulvate for the growth of arabidopsis provides potassium element, on the other hand since it is with high load amount and physiological activity,
Be conducive to improve soil hardening situation, improve absorptivity of the arabidopsis to nutriment, promote arabidopsis germinating growth；It is soluble
The effect of starch is to provide part carbon and plays the role of water-retaining agent, because soluble starch forms jelly after boiling
Matter is used in combination with potassium fulvate, can reduce the turnover rate of soil moisture.
(2) present invention prepares fermented maize slurry and fermented soybean milk using the method for fermentation, by big point in corn and soya-bean milk
Sub- protein degradation is small molecule amino acid, forms natural amino acid complex, can promote the growth of arabidopsis.Niblet and
Ferment again after soybean is soaking, from table 1-3 the data of embodiment 4 can be seen that preparation drought resistance agent it is better.
Specific implementation mode
The present invention is described in detail With reference to embodiment, it is to be understood that protection scope of the present invention is simultaneously
It is not restricted by specific implementation.The test method of actual conditions is not specified in the following example, usually according to normal condition
Operation, the experiment material source being not specified is commercially available, due to not being related to inventive point, therefore its step is not described in detail.
The present invention provides a kind of arabidopsis drought resistance agents, are made of the raw material components of following parts by weight：Fermented maize slurry 50
~100 parts, 30~45 parts of fermented soybean milk, 5~15 parts of potassium fulvate, 1~5 part of gibberellin, 1~3 part of soluble starch；
The fermented maize slurry is the niblet that will be sterilized, sterile water, bacillus subtilis microbial agent and Bifidobacterium microbial inoculum
Mixing is fermented, and sterilizing adds water to stir, the filtrate collected after filtering；
The fermented soybean milk is that the soybean that will be sterilized, sterile water are mixed with bacillus subtilis microbial agent, is fermented, and sterilizing adds
Water stirs, the filtrate collected after filtering.
Based on same inventive concept, the present invention also provides a kind of preparation method of arabidopsis drought resistance agent, including it is following
Step：
S1 prepares fermented maize slurry
By the niblet of disinfection, sterile water, bacillus subtilis microbial agent and Bifidobacterium microbial inoculum according to 1000:10~15:
3:5 weight ratio mixing, 20-30 DEG C of fermentation 3d, sterilizing are ground into corn steep liquor, are then added and are equivalent to 50 times of corn steep liquor
The water of quality stirs, filtering, and the filtrate of collection is fermented maize slurry, filter residue can be used as arabidopsis culture organic fertilizer or
Arabidopsis culture substrate；
S2 prepares fermented soybean milk
By the soybean of disinfection, sterile water and bacillus subtilis microbial agent according to 1000:10~15:3 weight ratio is mixed
It closes, 20-30 DEG C of fermentation 5d, sterilizing is ground into soya-bean milk, the water for being equivalent to 50 times of quality of soya-bean milk is then added, stir, filter, receives
The filtrate of collection is fermented soybean milk, and filter residue can be used as the organic fertilizer or arabidopsis culture substrate of arabidopsis culture；
S3 prepares drought resistance agent
Each component is weighed by following parts by weight：50~100 parts of fermented maize slurry, 30~45 parts of fermented soybean milk, potassium fulvate 5
~15 parts, 1~5 part of gibberellin, 1~3 part of soluble starch；The each component mixing that will be weighed, 5~20min is boiled in stirring, cold
But, it filters, the filtrate of collection is to obtain arabidopsis drought resistance agent.
Based on same inventive concept, the present invention also provides a kind of application method of arabidopsis drought resistance agent, including it is following
Step：Arabidopsis seed is soaked in 18h in the arabidopsis drought resistance agent, the seed after impregnating is then taken out, is collected simultaneously leaching
The drought resistance agent of seed is steeped；The drought resistance agent of seed will have been impregnated with arabidopsis culture soil matrix according to 1:500 parts by weight are mixed
It is even, obtain culture substrate, the seed after sowing immersion.
Specifically include following embodiment.
Embodiment 1
A kind of arabidopsis drought resistance agent, is made of the raw material components of following parts by weight：Fermented maize starches 60 parts, fermented soybean milk 40
Part, 10 parts of potassium fulvate, 3 parts of gibberellin, 2 parts of soluble starch.The preparation method of the bacillus subtilis microbial agent is as follows：It will
Bacillus subtilis seed liquor is inoculated in by 5% inoculum concentration of volume fraction in fermentation tank, in 30 DEG C, speed of agitator 200r/min trainings
It supports for 24 hours, the rice bran absorption through 80 mesh of the zymotic fluid of acquisition, dry bacillus subtilis microbial agent；Bifidobacterium microbial inoculum and withered grass
The preparation method of gemma bacillus agent is identical；Viable count in bacillus subtilis microbial agent and Bifidobacterium microbial inoculum is
1010cfu/g.The arabidopsis drought resistance agent is prepared according to the following steps：
S1 prepares fermented maize slurry
By the niblet of disinfection, sterile water, bacillus subtilis microbial agent and Bifidobacterium microbial inoculum according to 1000:10:3:5
Weight ratio mixes, and 20 DEG C of fermentations 3d, boiling sterilization 20min are ground into corn steep liquor, are then added and are equivalent to 50 times of corn steep liquor
The water of quality, 200r/min stir 30min, flame filter press filtering, and filter cloth mesh number is 80 mesh, and the filtrate of collection is to ferment
Corn steep liquor；
S2 prepares fermented soybean milk
By the soybean of disinfection, sterile water and bacillus subtilis microbial agent according to 1000:10:3 weight ratio mixing, 20
DEG C fermentation 5d, boiling sterilization 20min is ground into soya-bean milk, and the water for being equivalent to 50 times of quality of soya-bean milk, 200r/min stirrings is then added
30min, flame filter press filtering, filter cloth mesh number is 80 mesh, and the filtrate of collection is fermented soybean milk；
Wherein in S1 and S2, niblet and soybean disinfection way are：Liquor natrii hypochloritis's soaking disinfection of 0.5g/100ml
15min is cleaned after sanitized with distilled water, then carries out subsequent step again；
S3 prepares drought resistance agent
Each component is weighed by following parts by weight：Fermented maize starch 60 parts, it is 40 parts of fermented soybean milk, 10 parts of potassium fulvate, red mould
Plain 3 parts, 2 parts of soluble starch；The each component mixing that will be weighed, stirring are boiled 5min, are cooled to room temperature, flame filter press mistake
Filter, filter cloth mesh number are 80 mesh, and the filtrate of collection is to obtain arabidopsis drought resistance agent.
Embodiment 2
A kind of arabidopsis drought resistance agent, is made of the raw material components of following parts by weight：Fermented maize starches 50 parts, fermented soybean milk 45
Part, 15 parts of potassium fulvate, 5 parts of gibberellin, 3 parts of soluble starch.The preparation method of the bacillus subtilis microbial agent is as follows：It will
Bacillus subtilis seed liquor is inoculated in by 3% inoculum concentration of volume fraction in fermentation tank, in 35 DEG C of temperature, speed of agitator 200r/
Min cultivates 18h, the rice bran absorption through 80 mesh of the zymotic fluid of acquisition, dry bacillus subtilis microbial agent；Bifidobacterium microbial inoculum with
The preparation method of bacillus subtilis microbial agent is identical；Viable count in bacillus subtilis microbial agent and Bifidobacterium microbial inoculum is
1010cfu/g.The arabidopsis drought resistance agent is prepared according to the following steps：
S1 prepares fermented maize slurry
By the niblet of disinfection, sterile water and bacillus subtilis microbial agent, Bifidobacterium microbial inoculum according to 1000:12:3:5
Weight ratio mixes, and 30 DEG C of fermentations 3d, boiling sterilization 20min are ground into corn steep liquor, are then added and are equivalent to 50 times of corn steep liquor
The water of quality, 200r/min stir 30min, flame filter press filtering, and filter cloth mesh number is 80 mesh, and the filtrate of collection is to ferment
Corn steep liquor；
S2 prepares fermented soybean milk
By the soybean of disinfection, sterile water and bacillus subtilis microbial agent according to 1000:12:3 weight ratio mixing, 30
DEG C fermentation 5d, boiling sterilization 20min is ground into soya-bean milk, and the water for being equivalent to 50 times of quality of soya-bean milk, 200r/min stirrings is then added
30min, flame filter press filtering, filter cloth mesh number is 80 mesh, and the filtrate of collection is fermented soybean milk；
Wherein in S1 and S2, niblet and soybean disinfection way are：Liquor natrii hypochloritis's soaking disinfection of 0.5g/100ml
15min is cleaned after sanitized with distilled water, then carries out subsequent step again；
S3 prepares drought resistance agent
Each component is weighed by following parts by weight：Fermented maize starch 50 parts, it is 45 parts of fermented soybean milk, 15 parts of potassium fulvate, red mould
Plain 5 parts, 3 parts of soluble starch；The each component mixing that will be weighed, stirring are boiled 20min, are cooled to room temperature, flame filter press mistake
Filter, filter cloth mesh number are 80 mesh, and the filtrate of collection is to obtain arabidopsis drought resistance agent.
Embodiment 3
A kind of arabidopsis drought resistance agent, is made of the raw material components of following parts by weight：Fermented maize starches 100 parts, fermented soybean milk
30 parts, 5 parts of potassium fulvate, 1 part of gibberellin, 1 part of soluble starch.The preparation method of the bacillus subtilis microbial agent is as follows：
Bacillus subtilis seed liquor is inoculated in by 4% inoculum concentration of volume fraction in fermentation tank, in 32 DEG C of temperature, speed of agitator
200r/min cultivates 20h, the rice bran absorption through 80 mesh of the zymotic fluid of acquisition, dry bacillus subtilis microbial agent；Bifidobacterium
Microbial inoculum is identical as the preparation method of bacillus subtilis microbial agent；Viable count in bacillus subtilis microbial agent and Bifidobacterium microbial inoculum
It is 1010cfu/g.The arabidopsis drought resistance agent is prepared according to the following steps：
S1 prepares fermented maize slurry
By the niblet of disinfection, sterile water and bacillus subtilis microbial agent, Bifidobacterium microbial inoculum according to 1000:15:3:5
Weight ratio mixes, and 25 DEG C of fermentations 3d, boiling sterilization 20min are ground into corn steep liquor, are then added and are equivalent to 50 times of corn steep liquor
The water of quality, 200r/min stir 30min, flame filter press filtering, and filter cloth mesh number is 80 mesh, and the filtrate of collection is to ferment
Corn steep liquor；
S2 prepares fermented soybean milk
By the soybean of disinfection, sterile water and bacillus subtilis microbial agent according to 1000:15:3 weight ratio mixing, 25
DEG C fermentation 5d, boiling sterilization 20min is ground into soya-bean milk, and the water for being equivalent to 50 times of quality of soya-bean milk, 200r/min stirrings is then added
30min, flame filter press filtering, filter cloth mesh number is 80 mesh, and the filtrate of collection is fermented soybean milk；
Wherein in S1 and S2, niblet and soybean are sterilized with carbendazim, after sanitized with distilled water clean, then again into
Row subsequent step；
S3 prepares drought resistance agent
Each component is weighed by following parts by weight：Fermented maize starch 60 parts, it is 40 parts of fermented soybean milk, 10 parts of potassium fulvate, red mould
Plain 3 parts, 2 parts of soluble starch；The each component mixing that will be weighed, stirring are boiled 15min, are cooled to room temperature, flame filter press mistake
Filter, filter cloth mesh number are 80 mesh, and the filtrate of collection is to obtain arabidopsis drought resistance agent.
Embodiment 4
A kind of arabidopsis drought resistance agent, is made of the raw material components of following parts by weight：Fermented maize starches 80 parts, fermented soybean milk 35
Part, 12 parts of potassium fulvate, 4 parts of gibberellin, 2 parts of soluble starch.The bacillus subtilis microbial agent and Bifidobacterium microbial inoculum are equal
It is commercially available；And the viable count in bacillus subtilis microbial agent and Bifidobacterium microbial inoculum is 2 × 1010cfu/g.The arabidopsis is anti-
Non-irrigated agent is prepared according to the following steps：
S1 prepares fermented maize slurry
By the niblet of disinfection, sterile water and bacillus subtilis microbial agent, Bifidobacterium microbial inoculum according to 1000:15:3:5
Weight ratio mixes, and 25 DEG C of fermentations 3d, boiling sterilization 20min are ground into corn steep liquor, are then added and are equivalent to 50 times of corn steep liquor
The water of quality, 200r/min stir 30min, flame filter press filtering, and filter cloth mesh number is 80 mesh, and the filtrate of collection is to ferment
Corn steep liquor；
S2 prepares fermented soybean milk
By the soybean of disinfection, sterile water and bacillus subtilis microbial agent according to 1000:15:3 weight ratio mixing, 25
DEG C fermentation 5d, boiling sterilization 20min is ground into soya-bean milk, and the water for being equivalent to 50 times of quality of soya-bean milk, 200r/min stirrings is then added
30min, flame filter press filtering, filter cloth mesh number is 80 mesh, and the filtrate of collection is fermented soybean milk；
Wherein in S1 and S2, niblet and soybean disinfection way are：Liquor natrii hypochloritis's soaking disinfection of 0.5g/100ml
15min is cleaned after sanitized with distilled water, then carries out subsequent step again；
It wherein in S1 and S2, after the niblet of disinfection is impregnated 4h in water, drains away the water, is then used further to prepare fermentation
Corn steep liquor；It after the soybean of disinfection is impregnated 5h in water, drains away the water, is then used further to prepare fermented soybean milk；
S3 prepares drought resistance agent
Each component is weighed by following parts by weight：Fermented maize starch 80 parts, it is 35 parts of fermented soybean milk, 12 parts of potassium fulvate, red mould
Plain 4 parts, 2 parts of soluble starch；The each component mixing that will be weighed, stirring are boiled 10min, are cooled to room temperature, flame filter press mistake
Filter, filter cloth mesh number are 80 mesh, and the filtrate of collection is to obtain arabidopsis drought resistance agent.
In above-described embodiment 1-4, Bifidobacterium is enough from Chinese microorganism strain preservation management committee with bacillus subtilis
Member can common micro-organisms center.
Below with the drought resistance agent of embodiment 1-4 as experimental group, its drought resistance function to arabidopsis is investigated.
One, arabidopsis Seeds Germination Tests
We choose 100 arabidopsis seeds, the liquor natrii hypochloritis soaking disinfection 15min through 0.5g/100ml, then
It is cleaned with distilled water, the drought resistance agent seed soaking 18h of embodiment 1-4 is respectively adopted, with distilled water seed soaking 18h A groups as a contrast.Control
The drought resistance agent of B groups is only fermented maize slurry；The drought resistance agent for compareing C groups is only fermented soybean milk.
Then seed after above-mentioned immersion used PEG-8000 to mediate agar medium simulation water through 4 DEG C of low-temperature treatments 2 days
Divide stress, investigates the growth of Arabidopsis thaliana Seedlings, specific practice is：Seed is put into the bottoms 90mm and is lined in the culture dish of filter paper,
Per 50 seeds of ware, it is then respectively adding the PEG-8000 solution Simulating water stress of 10g/100ml.It is soaked seed with distilled water, and
It is blank control group not add PEG-8000 solution.Seed sprouting situation and growth of seedling situation are observed after cultivating 6d.Knot
Fruit is as shown in table 1.As shown in Table 1, compared with compareing A groups, the germination percentage of each group is improved, and main root length is also
Increase, illustrates that the drought resistance agent of fermented maize slurry, fermented soybean milk and embodiment 1-4 all have certain drought resisting effect.
1 arabidopsis seed moisture content stress experiment of table
| Processing mode | Germination percentage/% | Main root length/mm |
| Embodiment 1 | 91 | 10 |
| Embodiment 2 | 92 | 9 |
| Embodiment 3 | 92 | 9 |
| Embodiment 4 | 90 | 11 |
| Compare A groups | 80 | 3 |
| Compare B groups | 85 | 5 |
| Compare C groups | 84 | 6 |
| Blank control group | 95 | 13 |
Two, Arabidopsis thaliana Seedlings drought resisting is tested
Specific processing mode is as follows：By the arabidopsis drought resistance agent of embodiment 1-4 groups respectively with arabidopsis culture soil matrix
According to 1:500 parts by weight are mixed thoroughly, and arabidopsis seed is then sowed.Control A groups are directly seeded in arabidopsis culture soil.It is right
According to B groups using fermented maize slurry and arabidopsis culture soil matrix according to 1:500 parts by weight are mixed thoroughly.C groups are compareed to use
Be fermented soybean milk with arabidopsis culture soil matrix according to 1:500 parts by weight are mixed thoroughly.The arabidopsis culture that this experiment is selected
Soil is conventional rural area soil, and after growing 3 true leaves, it is 50% to control water content in soil using weight method, carries out the moisture side of body
Compel experiment.After planting, control soil moisture content is maximum water-holding capacity to the seed of control A groups, as blank control group.After cultivating 7d
The growing state of seedling is observed, the results are shown in Table 2.As shown in Table 2, under Water Stress Conditions, the kind of A groups is compareed
Sub- germination percentage, survival rate of seedling are and leaf relative water content is minimum, and other groups are improved.Illustrate that fermented maize is starched
Use with fermented soybean milk can keep arabidopsis moisture.
2 Arabidopsis thaliana Seedlings water stress of table is tested
| Processing mode | Germination percentage/% | Survival rate of seedling/% | Leaf relative water content/% |
| Embodiment 1 | 90 | 71 | 85 |
| Embodiment 2 | 88 | 69 | 84 |
| Embodiment 3 | 89 | 70 | 85 |
| Embodiment 4 | 90 | 72 | 86 |
| Compare A groups | 78 | 52 | 80 |
| Compare B groups | 83 | 62 | 83 |
| Compare C groups | 84 | 63 | 83 |
| Blank control group | 95 | 90 | 90 |
Three, Arabidopsis thaliana Seedlings crop leaf measuring is tested
Take the seedling leaves of " Arabidopsis thaliana Seedlings drought resisting experiment ", detect the proline content of blade in each group maturation plant,
Mda content, excised leaf percentage of water loss and Chlorophyll SPAD Reading, the results are shown in Table 3.Table 3 is the results show that embodiment 1-4
Group, compareing A, B, C group and the mda content of blank control group, proline, Chlorophyll SPAD Reading content has in various degree
Increase, in vitro 3h leaves water loss rate has different degrees of reduction.Proline and malonaldehyde are all the important objects of plant drought
Matter, closely related with the water holding capacity of plant leaf, the increase of chlorophyll test value contributes to the photosynthesis of plant, promotes plant
Growth.
The ripe plant leaf test experience of table 3
In addition, it has been found that soluble starch forms colloid substance after boiling, and is used in combination with potassium fulvate, can reduce soil
The loss of moisture in earth.Experiment is found, under same environmental condition, 1 arabidopsis drought resistance agent of embodiment is pressed with rural area soil respectively
According to 1:After 500 parts by weight are mixed thoroughly, 10g natural air drying 1h, moisture is taken to reduce 40.5%；The another rural area soil for taking considerable moisture with
Distilled water is according to 500:After 1 parts by weight are mixed thoroughly, 10g natural air drying 1h, moisture is taken to reduce 61.2%.
Although preferred embodiments of the present invention have been described, it is created once a person skilled in the art knows basic
Property concept, then additional changes and modifications may be made to these embodiments.So it includes excellent that the following claims are intended to be interpreted as
It selects embodiment and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (7)
1. a kind of arabidopsis drought resistance agent, which is characterized in that be made of the raw material components of following parts by weight：Fermented maize slurry 50~
100 parts, 30~45 parts of fermented soybean milk, 5~15 parts of potassium fulvate, 1~5 part of gibberellin, 1~3 part of soluble starch；
The fermented maize slurry is that the niblet, sterile water, bacillus subtilis microbial agent that will be sterilized are mixed with Bifidobacterium microbial inoculum,
Fermentation, sterilizing, adds water to stir, the filtrate collected after filtering；
The fermented soybean milk is that the soybean that will be sterilized, sterile water are mixed with bacillus subtilis microbial agent, is fermented, and sterilizing adds water to stir
It mixes, the filtrate collected after filtering.
2. arabidopsis drought resistance agent according to claim 1, which is characterized in that be made of the raw material components of following parts by weight：
60 parts of fermented maize slurry, 40 parts of fermented soybean milk, 10 parts of potassium fulvate, 3 parts of gibberellin, 2 parts of soluble starch.
3. the preparation method of arabidopsis drought resistance agent according to claim 1, which is characterized in that include the following steps：
S1 prepares fermented maize slurry
By the niblet of disinfection, sterile water, bacillus subtilis microbial agent and Bifidobacterium microbial inoculum according to 1000:10~15:3:5
Weight ratio mixes, and 20-30 DEG C of fermentation 3d, sterilizing is ground into corn steep liquor, is then added and is equivalent to 50 times of quality of corn steep liquor
Water stirs, filtering, and the filtrate of collection is fermented maize slurry；
S2 prepares fermented soybean milk
By the soybean of disinfection, sterile water and bacillus subtilis microbial agent according to 1000:10~15:3 weight ratio mixing,
20-30 DEG C of fermentation 5d, sterilizing are ground into soya-bean milk, the water for being equivalent to 50 times of quality of soya-bean milk are then added, stir, filtering, collection
Filtrate is fermented soybean milk；
S3 prepares drought resistance agent
Each component is weighed by following parts by weight：50~100 parts of fermented maize slurry, 30~45 parts of fermented soybean milk, potassium fulvate 5~15
Part, 1~5 part of gibberellin, 1~3 part of soluble starch；5~20min is boiled in each component mixing that will be weighed, stirring, cooling, mistake
Filter, the filtrate of collection are to obtain arabidopsis drought resistance agent.
4. the preparation method of arabidopsis drought resistance agent according to claim 3, which is characterized in that in water by the niblet of disinfection
It after impregnating 3~5h, drains away the water, is then used further to prepare fermented maize slurry；After the soybean of disinfection is impregnated 4~6h in water,
It drains away the water, is then used further to prepare fermented soybean milk.
5. according to the preparation method of the arabidopsis drought resistance agent of claim 3 or 4, which is characterized in that the item to sterilize in S1 and S2
Part is boiling sterilization 20min.
6. according to the preparation method of the arabidopsis drought resistance agent of claim 3 or 4, which is characterized in that stirred in S1 and S2 when
Between be 30min, speed of agitator 200r/min.
7. the application method of arabidopsis drought resistance agent described in a kind of claim 1, which is characterized in that include the following steps：By quasi- south
Canola seed is soaked in 18h in the arabidopsis drought resistance agent, then takes out the seed after impregnating, and is collected simultaneously and has impregnated the anti-of seed
Non-irrigated agent；The drought resistance agent of seed will have been impregnated with arabidopsis culture soil matrix according to 1:500 parts by weight are mixed thoroughly, and culture medium is obtained
Matter, the seed after sowing immersion.
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