CN109757247B - Application of porphyra polysaccharide in improving drought resistance of plants - Google Patents
Application of porphyra polysaccharide in improving drought resistance of plants Download PDFInfo
- Publication number
- CN109757247B CN109757247B CN201910206810.5A CN201910206810A CN109757247B CN 109757247 B CN109757247 B CN 109757247B CN 201910206810 A CN201910206810 A CN 201910206810A CN 109757247 B CN109757247 B CN 109757247B
- Authority
- CN
- China
- Prior art keywords
- drought
- porphyra polysaccharide
- plant
- plants
- resistant agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides application of porphyra polysaccharide in drought resistance of plants, belonging to the technical field of plant growth regulation. According to the invention, the porphyra polysaccharide can adjust the osmotic pressure of plant cells under drought stress, so that the water content of leaves is ensured; can also improve the activity of antioxidant enzymes in plants and relieve the peroxidation of cytoplasmic membranes caused by drought stress, thereby improving the comprehensive resistance of the plants to the drought stress and ensuring the normal growth and development of the plants. The invention takes the laver as the raw material, has rich sources, low cost and environmental protection.
Description
Technical Field
The invention relates to the technical field of plant growth regulators, in particular to application of porphyra polysaccharide in improving drought resistance of plants.
Background
Drought is one of the major environmental stresses in agricultural production, which seriously affects the growth and yield of crops, and causes losses to crops which are the first of all abiotic stresses. Wheat is the second major food crop in China, and the yield of wheat is always influenced by drought stress. Therefore, the improvement of the drought resistance of crops has important practical significance and application value for ensuring the high and stable yield of crops, and ensuring the national grain safety and sustainable production.
Abscisic acid is one of the most important plant hormones, widely exists in plants and participates in the regulation of each stage of plant growth and development. Under the condition of water shortage, the abscisic acid level in the plant body is rapidly increased, and the water evaporation is reduced by controlling the closure of pores on the plant leaves, so that the drought resistance effect is achieved. However, the effect of the abscisic acid is single, and the effect of overall drought resistance cannot be achieved.
Disclosure of Invention
The invention aims to provide application of porphyra polysaccharide in improving drought resistance of plants, wherein the porphyra polysaccharide can improve the comprehensive resistance of the plants to drought stress and ensure the normal growth and development of the plants.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides application of porphyra polysaccharide in improving drought resistance of plants.
Preferably, the porphyra polysaccharide is prepared by the following method:
1) mixing thallus Porphyrae powder with water, extracting, and filtering to obtain supernatant;
2) dialyzing the supernatant obtained in the step 1), concentrating, mixing the obtained concentrated solution with ethanol, standing, and centrifuging to obtain a precipitate;
3) and (3) drying the precipitate obtained in the step 2) to obtain the porphyra polysaccharide.
Preferably, the molecular weight of the porphyra polysaccharide is 1-370.5 kDa.
Preferably, the plants include grain crops and vegetables.
The invention also provides a plant drought-resistant agent, which comprises the porphyra polysaccharide and water in the scheme; the concentration of the porphyra polysaccharide in the plant drought resistant agent is 1-100 mg/L.
Preferably, the plant drought-resistant agent further comprises a surfactant.
Preferably, the concentration of the surfactant in the plant drought-resistant agent is 1000-20000 mg/L.
Preferably, the surfactant comprises one or more of tween series, span series, OP series and NP series.
The invention provides a use method of the plant drought-resistant agent in the scheme, which comprises the following steps: the plant drought resistant agent is applied to the plant during the seed germination stage and/or seedling stage of the plant.
Preferably, the mode of application includes seed soaking, root irrigation and spraying.
The invention has the beneficial effects that: the invention provides application of porphyra polysaccharide in improving drought resistance of plants. According to the invention, the porphyra polysaccharide can adjust the osmotic pressure of plant cells under drought stress, so that the water content of leaves is ensured; can also improve the activity of antioxidant enzymes in plants and relieve the peroxidation of cytoplasmic membranes caused by drought stress, thereby improving the comprehensive resistance of the plants to the drought stress and ensuring the normal growth and development of the plants. The invention takes the laver as the raw material, has rich sources, low cost and environmental protection.
Drawings
FIG. 1 shows the change in water content of wheat leaves under drought stress;
FIG. 2 is the change in relative conductivity of wheat leaves under drought stress;
FIG. 3 is the change in wheat root activity under drought stress;
FIG. 4 is the effect of drought stress on chlorophyll content of cucumber leaves;
FIG. 5 is a graph of the effect of drought stress on malondialdehyde content in cucumber leaves;
FIG. 6 is a graph showing the effect of drought stress on SOD activity in cucumber leaves.
Detailed Description
The invention provides an application of porphyra polysaccharide in improving the drought resistance of plants; the molecular weight of the porphyra polysaccharide is preferably 1-370.5 kDa, and more preferably 48.8 kDa; the porphyra polysaccharide is preferably prepared by a water extraction and alcohol precipitation method. In the specific implementation process of the invention, the porphyra polysaccharide is preferably prepared by adopting the following method:
1) mixing thallus Porphyrae powder with water, extracting, and filtering to obtain supernatant;
2) dialyzing the supernatant obtained in the step 1), concentrating, mixing the obtained concentrated solution with ethanol, standing, and centrifuging to obtain a precipitate;
3) and (3) drying the precipitate obtained in the step 2) to obtain the porphyra polysaccharide.
Mixing laver powder with water, extracting and filtering to obtain supernatant; the particle size of the laver powder is preferably 50-80 meshes, and more preferably 60-70 meshes; the mass volume ratio of the laver powder to the water is preferably 80-120 g: 3-5L, and more preferably 100g: 4L; the water is preferably distilled water; the extraction temperature is preferably 90-110 ℃, and more preferably 100 ℃; the extraction time is preferably 3-5 h, and more preferably 4 h; the filtration is preferably carried out by using a yarn silk and diatomite,
in the specific implementation process of the invention, the residue is filtered by 40-mesh bolting silk, and then the diatomite is used for suction filtration. Wetting filter paper with distilled water, starting a water pump to enable the filter paper to be tightly adsorbed on a funnel, adding diatomite with the thickness of 1-2 cm, uniformly spreading the diatomite as much as possible, adding a small amount of distilled water for wetting, and slowly pouring the filtrate for suction filtration.
After obtaining the supernatant, dialyzing and concentrating the supernatant, mixing the obtained concentrated solution with ethanol, standing, centrifuging and obtaining a precipitate; the cutoff molecular weight of the dialysis bag is preferably 3000-4000 Da, and more preferably 3500 Da; the dialysis time is preferably 36-60 h, and more preferably 48 h; the concentration is preferably rotary evaporation concentration; the volume ratio of the concentrated solution after concentration to the solution before concentration is preferably 1: 3-4; the volume ratio of the concentrated solution to the ethanol is preferably 1: 3-5, and more preferably 1: 4; the standing time is preferably 9-14 h, and more preferably 12 h; the temperature of the standing is preferably 4 ℃; the centrifugation time is preferably 5min to 10min, and more preferably 8 min; the rotation speed of the centrifugation is preferably 4000g/min to 6000g/min, and more preferably 5000 g/min.
After the precipitate is obtained, the precipitate is dried to obtain the porphyra polysaccharide; the drying mode is preferably freeze drying; the temperature of the freeze drying is preferably-70 ℃ to-80 ℃; the freeze-drying time is preferably 48 to 72 hours, and more preferably 60 hours.
After the porphyra polysaccharide is obtained, the invention preferably further comprises the steps of mixing the porphyra polysaccharide, water, concentrated hydrochloric acid and hydrogen peroxide to obtain a mixed solution, and performing microwave degradation on the mixed solution to obtain the low-molecular-weight porphyra polysaccharide.
In the invention, the laver polysaccharide accounts for 1-3% of water by mass, and is preferably 2%; the concentration of the hydrochloric acid in the mixed solution is preferably 0.05-1 mol/L, and more preferably 0.1 mol/L; the volume percentage content of the hydrogen peroxide in the mixed solution is preferably 0.2-0.4%, and more preferably 0.3%; the power of the microwave is preferably 500-800W, and more preferably 600-700W; the degradation temperature is preferably 60-80 ℃, and more preferably 70 ℃; the degradation time is preferably 5-60 min, and more preferably 10-40 min.
In the present invention, the plant preferably includes grain crops and vegetables; the cereal crops preferably comprise wheat, corn; the vegetables preferably comprise cucumber and rape.
The invention also provides a plant drought-resistant agent, which comprises the porphyra polysaccharide and water in the scheme; the concentration of the porphyra polysaccharide is 1-100 mg/L.
In the present invention, the plant drought-resistant agent preferably further comprises a surfactant; the concentration of the surfactant is preferably 1000-20000 mg/L, and more preferably 5000-10000 mg/L; the surfactant preferably comprises one or more of the tween series, span series, OP series and NP series.
In the present application, the surfactant functions as an emulsifier to uniformly disperse the polysaccharide in the solution.
In the invention, the plant drought-resistant agent is preferably prepared by the following method: mixing the porphyra polysaccharide with water to obtain a plant drought resistant agent; or mixing the laver polysaccharide, water and a surfactant to obtain the plant drought-resistant agent; the mixing is preferably accompanied by stirring; the time and temperature of the mixing are not particularly limited, and the uniform mixing is taken as the standard.
The invention provides a use method of the plant drought-resistant agent in the scheme, which comprises the following steps: applying the plant drought resistance agent to a plant; the period of application is preferably a seed germination period and/or a seedling period, more preferably a seedling period; the application mode preferably comprises seed soaking, root irrigation and spraying, and more preferably spraying; when the application mode is seed soaking, the seed soaking time is preferably 6-12 hours, and more preferably 8-10 hours.
In the specific implementation process of the invention, when the plant drought-resistant agent does not contain a surfactant, the application mode of the plant drought-resistant agent is seed soaking; when the plant drought-resistant agent comprises a surfactant, the application mode of the plant drought-resistant agent is spraying or root irrigation; the application amount of the plant drought resistant agent is preferably 45-50L/mu.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1A method for preparing Porphyra polysaccharide
Mixing 100g dried thallus Porphyrae powder with 4L distilled water, extracting at 100 deg.C for 4 hr, and filtering with gauze and diatomaceous earth to obtain supernatant. Dialyzing the filtrate for 48h, carrying out rotary evaporation and concentration to 1/4 with the original volume, adding ethanol with the volume of 4 times into the concentrated solution, carrying out overnight precipitation at 4 ℃, centrifuging to obtain a precipitate, and freeze-drying to obtain a white powdery product, namely porphyra polysaccharide, wherein the molecular weight of the porphyra polysaccharide is 370.5kDa, and the porphyra polysaccharide is named as PP.
Example 2A method for preparing Low molecular weight Porphyra polysaccharide
Dissolving the porphyra polysaccharide in example 1 in distilled water, wherein the mass percent of the porphyra polysaccharide in the water is 2%, adding concentrated hydrochloric acid to ensure that the final concentration of the hydrochloric acid is 0.1mol/L and the volume percent of hydrogen peroxide is 0.3%, setting the microwave power to be 600W, setting the reaction temperature to be 70 ℃, and carrying out degradation in a microwave reactor for 10min to obtain the low-molecular-weight porphyra polysaccharide with the molecular weight of 29.0kDa, wherein the porphyra polysaccharide is named as DPP 1.
Example 3A method for preparing Low molecular weight Porphyra polysaccharide
Dissolving the porphyra polysaccharide in example 1 in distilled water, wherein the mass percent of the porphyra polysaccharide in the water is 2%, adding concentrated hydrochloric acid to ensure that the final concentration of the hydrochloric acid is 0.1mol/L and the volume percent of hydrogen peroxide is 0.3%, setting the microwave power to be 600W, setting the reaction temperature to be 70 ℃, and carrying out degradation in a microwave reactor for 5min to obtain the low-molecular-weight porphyra polysaccharide with the molecular weight of 48.8kDa, wherein the porphyra polysaccharide is named as DPP 2.
Example 4A method for preparing Low molecular weight Porphyra polysaccharide
Dissolving the porphyra polysaccharide in example 1 in distilled water, wherein the mass percent of the porphyra polysaccharide in the water is 2%, adding concentrated hydrochloric acid to ensure that the final concentration of the hydrochloric acid is 0.1mol/L and the volume percent of hydrogen peroxide is 0.3%, setting the microwave power to be 600W, setting the reaction temperature to be 70 ℃, and carrying out degradation in a microwave reactor for 60min to obtain the low-molecular-weight porphyra polysaccharide with the molecular weight of 3.2kDa, wherein the porphyra polysaccharide is named as DPP 3.
Example 5A method for preparing a drought resistant agent for plants
Weighing 100mg of porphyra polysaccharide, dissolving in 800mL of distilled water, finally diluting to a constant volume of 1L with the distilled water, and stirring uniformly to obtain the salt-resistant regulator.
Example 6A method for preparing a drought resistant agent for plants
Weighing 100mg of porphyra polysaccharide, dissolving the porphyra polysaccharide in 800mL of distilled water to prepare a porphyra polysaccharide solution, adding 1000mg of Tween-20, uniformly stirring, finally adding distilled water to a constant volume of 1L, and uniformly stirring to prepare the plant drought-resistant agent.
Example 7
The invention takes wheat, cucumber and corn as examples, wheat seedlings and cucumber seedlings in the seedling stage are respectively sprayed and root-irrigated with the plant drought-resistant agent (respectively taking PP, DPP1, DPP2 and DPP3 as raw materials) prepared by the preparation method of example 6, and corn seeds are soaked with the plant drought-resistant agent (respectively taking PP, DPP1, DPP2 and DPP3 as raw materials) prepared by the preparation method of example 5, and the specific experiments are as follows:
1. taking wheat as an example
The experimental method comprises the following steps:
soaking semen Tritici Aestivi in 1% NaClO for 10min, and repeatedly washing with distilled water. Soaking in distilled water for 6h, placing in dark at 25 deg.C for accelerating germination for 24h, selecting full and uniform seeds when wheat seeds are just white, and sowing in water culture dishes with 30 seeds per dish. Placing the culture medium in an illumination incubator for culture under the following culture conditions: light cycle 14h/l0h (day/night), temperature 27/20 deg.C (day/night), light intensity 800. mu. mol-2s-1Relative humidity 65. + -. 5%.
When the wheat seedlings grow to 2 leaves and 1 heart stage, the wheat seedlings are placed in nutrient solution containing 15% PEG6000 to be stressed for 24 hours, and then the plant drought-resistant agent prepared by the preparation method of the embodiment 6 is sprayed on the leaves of the wheat seedlings by respectively taking PP, DPP1, DPP2 and DPP3 as raw materials, so that the leaves are completely wet, but the liquid does not flow down, and the negative control group is sprayed with the same amount of distilled water containing the surfactant with the same concentration. The plant drought-resistant agent is sprayed once every other day and three times in total, during which the nutrient solution (containing 15% PEG6000) is changed, and after 7d, the influence of drought stress on the water content of wheat leaves, the influence on the permeability of leaf cell membranes and the influence on root system activity are determined.
The experimental results are as follows:
after the wheat seedlings are subjected to drought stress treatment for 7d, the whole wheat leaves in different treatment groups are respectively taken, cut off from the leaf base, weighed, quickly put into distilled water for soaking for 6h after weighing the fresh weight (initial fresh weight), taken out from the water, wiped to remove the redundant water on the surfaces of the leaves, and weighed to be saturated fresh weight. After deactivation of enzymes at 105 ℃ for 30min, drying to constant weight at 75 ℃, weighing (dry weight) and calculating the relative water content of the leaves:
leaf water content (%) - (initial fresh weight-dry weight)/initial fresh weight × 100%
Leaf relative water content (%) - (initial fresh weight-dry weight)/(saturated fresh weight-dry weight) × 100%
Leaf saturation water loss (%) -1-leaf relative water content (%);
the experimental results are shown in fig. 1, and it can be seen from fig. 1 that the relative water content of the wheat leaves of the drought stress treatment group is greatly reduced, the saturated water deficit is greatly increased, the relative water content of the wheat leaves of the drought stress treatment group sprayed with the plant drought-resistant agent is reduced, and the saturated water deficit is also reduced, wherein the relative water content of the wheat leaves of the DPP2 treatment group is the highest, which indicates that porphyra polysaccharide with different molecular weights used in the research can slow down the loss of the water content of the wheat leaves, and the porphyra polysaccharide with the molecular weight of 48.8kDa has the best effect.
And measuring the conductivity of the wheat leaves in different treatment groups after drought stress treatment of the wheat seedlings for 7 d. The experimental results are shown in fig. 2, and it can be seen from fig. 2 that under drought stress, cell membranes of wheat leaves are damaged, so that the relative conductivity of the leaves is increased, the conductivity of the wheat leaves in a drought stress group is 52.65%, the conductivities of the wheat leaves in a PP, DPP1, DPP2 and DPP3 treatment group are respectively 26.04%, 33.34%, 23.73% and 25.76%, and the relative conductivity values of the wheat leaves in the drought stress treatment group sprayed with the plant drought resistant agent are obviously lower than those in the drought stress treatment group not sprayed with the plant drought resistant agent, which indicates that the porphyra polysaccharide can protect the cell membranes of the leaves, prevent water loss in cells and has resistance to drought stress.
Thirdly, after the wheat seedlings are subjected to drought stress treatment for 7d, the roots of the wheat in different treatment groups are respectively taken, and the root activity of the roots is measured by adopting a TTC method. The experimental results are shown in FIG. 3, and it can be seen from FIG. 3 that the root activity of wheat under drought stress is obviously reduced, but the root activities of wheat in the PP, DPP1, DPP2 and DPP3 treatment groups are all higher than those of the normal group and are respectively 37.75, 169.67, 204.32 and 191.64 mu g g higher than those of the normal group-1h-1The wheat root system activity of the DPP2 treated group is strongest, which shows that the porphyra polysaccharide has the effects of enhancing the activity of root dehydrogenase, keeping the activity of the root system, promoting the water absorption capacity of the root and improving the resistance of the wheat to drought.
And fourthly, after the wheat seedlings are subjected to drought stress treatment for 7 days, respectively taking 20 wheat seedlings in different treatment groups, and measuring and counting the average plant height, root length, fresh weight and dry weight of each group of wheat. The experimental results are shown in table 1, and it can be seen from table 1 that the growth vigor of wheat under drought stress is weaker than that of the drought stress treatment group sprayed with the plant drought resistant agent, and the average plant height, root length and fresh weight of wheat in the DPP2 treatment group are higher than those of the other treatment groups, so that the porphyra polysaccharide also has the effect of promoting plant growth, and the porphyra polysaccharide with the molecular weight of 48.8kDa is more beneficial to plant growth.
TABLE 1 wheat seedling Biomass Change under drought stress
2. Take cucumber as an example
The experimental method comprises the following steps:
soaking cucumber seed in 1% NaClO for 10min, and distillingAnd repeatedly washing with water. Soaking in distilled water for 6h, placing in dark at 25 deg.C for accelerating germination for 24h, selecting full and uniform seeds when cucumber seeds are just white, and sowing in water culture dishes with 30 seeds per dish. Placing the culture medium in an illumination incubator for culture under the following culture conditions: light cycle 10h/l4h (day/night), temperature 28/18 deg.C (day/night), light intensity 300 μmolm-2s-1Relative humidity 65. + -. 5%.
When the cucumber seedlings grow to 5 leaves and 1 heart stage, respectively taking PP, DPP1, DPP2 and DPP3 as raw materials, respectively and uniformly mixing the plant drought-resistant agent A prepared by the preparation method of the embodiment 6 in a nutrient solution containing 15% PEG6000 at a concentration of 1%, carrying out stress treatment on the cucumber seedlings, changing the plant drought-resistant agent and the nutrient solution (containing 15% PEG6000) once every other day, and determining the influence of the drought stress on physiological indexes of the cucumber seedlings such as chlorophyll content, Malondialdehyde (MDA) content, proline content, superoxide dismutase and the like after 7 days.
The experimental results are as follows:
after drought stress treatment of cucumber seedlings for 7d, respectively taking cucumber leaves at the same positions in different treatment groups, extracting chlorophyll by an ethanol method, measuring absorbance at 649 nm and 665nm by a spectrophotometry, and calculating the contents of chlorophyll a, chlorophyll b and total chlorophyll by the following formulas:
chlorophyll a concentration (mg/L) of 12.71 xA 665-2.59 xA 649
Chlorophyll b concentration (mg/L) 22.88 × a649-4.67 × a 665;
the experimental results are shown in fig. 4, and it can be seen from fig. 4 that the drought stress causes the chlorophyll a and chlorophyll b contents of the cucumber leaves to decrease, but the chlorophyll contents of the cucumber leaves of the PP, DPP1 and DPP3 treatment groups are not obviously different from those of the control group, and the chlorophyll content of the cucumber leaves of the DPP2 treatment group increases, which indicates that the porphyra polysaccharide can protect the leaves from decreasing of the chlorophyll content under the drought stress, and the porphyra polysaccharide with the molecular weight of 48.8kDa can increase the chlorophyll content of plants, thereby maintaining photosynthesis and improving the drought resistance of the leaves.
And measuring the MDA content of the cucumber leaves in different treatment groups after drought stress treatment of the cucumber seedlings for 7 d. The experimental result is shown in fig. 5, and it can be seen from fig. 5 that the cucumber leaf cell membranes in the drought-treated group are damaged, which results in that the MDA content of the leaves is obviously increased and is 2 times of that of the control group, but the MDA content of the cucumber leaves in the plant drought-resistant agent treated group is not obviously changed. The laver polysaccharide can reduce the malondialdehyde content of cucumber leaves under drought stress, protect plant cell membranes from oxidative damage, prevent cytoplasm loss and improve the resistance to drought stress.
And thirdly, after the wheat seedlings are subjected to drought stress treatment for 7d, measuring the SOD activity of the cucumber leaves in different treatment groups respectively. The results of the experiment are shown in FIG. 6, and it can be seen from FIG. 6 that SOD is the most important enzyme for plants to scavenge active oxygen. Under the drought stress stimulation, the SOD activity of the cucumber leaves of the drought treatment group is increased, but the SOD activity of the cucumber leaves treated by the plant drought resistant agent is higher than that of the drought stress group, wherein the SOD activity of the cucumber leaves of the DPP2 treatment group is the highest. The result shows that the porphyra polysaccharide can eliminate excessive active oxygen generated by peroxidation of membrane lipid, the porphyra polysaccharide with the molecular weight of 48.8kDa has the strongest capacity of eliminating the active oxygen, and can reduce oxidative damage of cell membranes and improve the drought resistance of plants.
3. Taking corn as an example
The experimental method comprises the following steps:
soaking semen Maydis in 1% NaClO for 10min, and repeatedly washing with distilled water. Respectively taking PP, DPP1, DPP2 and DPP3 as raw materials at room temperature, soaking the plant drought-resistant agent prepared by the preparation method of the example 5 in a concentration of 1%, and selecting full and uniform seeds in culture dishes with 30 seeds per dish. Soaking in a thermostat at 25 ℃ for 24h, washing with distilled water for 3 times, naturally air-drying, placing in a culture dish paved with 2 layers of filter paper, adding 10mL of distilled water, culturing in a thermostat at 26 ℃ in a dark environment until seeds germinate, and determining the germination rate, germination index and vitality index of the seeds.
Germination percentage (%) — germination number/seed number × 100 (measured at 7 d);
germination potential (%) — number of sprouts/number of seeds × 100 (first 2d measurement);
germination Index (GI) ═ Σ Gt/Dt (Gt denotes the number of sprouts of t d, Dt denotes the number of days to sprout);
vitality Index (VI) ═ GI × s (GI represents germination index, s represents average root length)
The experimental results are shown in table 2, and it can be seen from table 2 that the germination rate, the germination vigor, the germination index and the vigor index of the corn seeds are improved by the treatment of the plant drought-resistant agent. The germination rate, the germination potential, the germination index and the activity index of the corn seeds in the DPP2 treated group are obviously higher than those of the control group, and the germination rate, the germination potential, the germination index and the activity index of the corn seeds in the PP, DPP1 and DPP3 treated group are also higher than those of the control group, but the difference is not obvious. Therefore, the porphyra polysaccharide can promote the germination of seeds, and the porphyra polysaccharide with the molecular weight of 48.8kDa penetrates into the seeds to have the best effect.
TABLE 2 influence of plant drought-resistant Agents on maize seed Germination index
The plant drought-resistant agent obtained in the embodiment of the invention is applied to other plants, and also has good drought-resistant effect.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (3)
1. A plant drought-resistant agent is characterized in that the plant drought-resistant agent consists of porphyra polysaccharide, a surfactant and water; the concentration of the porphyra polysaccharide in the plant drought resistant agent is 1-100 mg/L;
the concentration of the surfactant in the plant drought-resistant agent is 1000-20000 mg/L;
the surfactant is tween-20;
the molecular weight of the porphyra polysaccharide is 48.8 kDa.
2. The method of using the plant drought resistant agent of claim 1, wherein the plant drought resistant agent is applied to a plant during seed germination and/or seedling stage of the plant.
3. The use according to claim 2, wherein the mode of application comprises seed soaking, root irrigation and spraying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910206810.5A CN109757247B (en) | 2019-03-19 | 2019-03-19 | Application of porphyra polysaccharide in improving drought resistance of plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910206810.5A CN109757247B (en) | 2019-03-19 | 2019-03-19 | Application of porphyra polysaccharide in improving drought resistance of plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109757247A CN109757247A (en) | 2019-05-17 |
| CN109757247B true CN109757247B (en) | 2021-07-06 |
Family
ID=66459442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910206810.5A Active CN109757247B (en) | 2019-03-19 | 2019-03-19 | Application of porphyra polysaccharide in improving drought resistance of plants |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109757247B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111316901A (en) * | 2020-05-07 | 2020-06-23 | 山西农业大学 | Application of spirulina polysaccharide and degradation product thereof in improving salt tolerance of plants |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535380A (en) * | 2013-10-28 | 2014-01-29 | 成都新朝阳作物科学有限公司 | Crop drought resistant agent as well as application thereof |
| CN105875611A (en) * | 2016-05-23 | 2016-08-24 | 中国农业科学院烟草研究所 | Plant salt resisting conditioning agent and preparation method thereof |
| CN109232762A (en) * | 2018-10-10 | 2019-01-18 | 汕头大学 | A kind of Porphyra haitanensis polysaccharide and preparation method thereof is applied with prebiotic activity |
-
2019
- 2019-03-19 CN CN201910206810.5A patent/CN109757247B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535380A (en) * | 2013-10-28 | 2014-01-29 | 成都新朝阳作物科学有限公司 | Crop drought resistant agent as well as application thereof |
| CN105875611A (en) * | 2016-05-23 | 2016-08-24 | 中国农业科学院烟草研究所 | Plant salt resisting conditioning agent and preparation method thereof |
| CN109232762A (en) * | 2018-10-10 | 2019-01-18 | 汕头大学 | A kind of Porphyra haitanensis polysaccharide and preparation method thereof is applied with prebiotic activity |
Non-Patent Citations (2)
| Title |
|---|
| 海带多糖在农业生产中应用;王杰等;《世界农药》;20111231;第33卷(第06期);全文 * |
| 海藻提取物的抗旱性研究;孙杰等;《海洋科学》;20060609(第06期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109757247A (en) | 2019-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106613702B (en) | Method for relieving drought stress of kiwi fruit trees | |
| Xu et al. | Effects of carboxymethyl chitosan on the growth and nutrient uptake in Prunus davidiana seedlings | |
| CN102674944B (en) | Nutrition drought resistant agent for plant | |
| CN107087641B (en) | Marine oligosaccharide biological preparation for improving salt resistance of crops and preparation method thereof | |
| CN107517995B (en) | Formula for improving storage capacity of hybrid rice seeds, preparation method and application | |
| Burdina et al. | Effect of the Substrate Composition on Yield and Quality of Basil (L.) | |
| CN107736374B (en) | Method for enhancing photosynthetic capacity and growth of cucumber seedlings | |
| CN109757247B (en) | Application of porphyra polysaccharide in improving drought resistance of plants | |
| Abbasi et al. | Using tragacanth gum mitigated the effects of drought stress on the black cumin (Nigella sativa) plant | |
| CN105961390A (en) | Application of methyl jasmonate in waterlogging resistance of plants | |
| CN105532280A (en) | Method for promoting growth of brassica chinensis L. | |
| Ayub et al. | Responses of different okra (Abelmoschus esculentus) cultivars to water deficit conditions | |
| CN115968884B (en) | Plant antifreeze agent containing curcumin and raffinose as well as preparation method and application thereof | |
| CN109105374B (en) | Corn seed initiator, preparation method thereof and method for promoting low-temperature germination resistance of corn seeds | |
| CN104026130B (en) | The anti-low temperature conditioning agent of a kind of corn | |
| Soedarjo et al. | Influence of growth media and bulbil sizes on plant growth and corm yield of porang (Amorphophallus muelleri Blume) | |
| Havrilyuk et al. | Effect of superabsorbent on soil moisture, productivity and some physiological and biochemical characteristics of basil | |
| Wang et al. | Physiological Response of Vitis Amurensis Rupr. Seedlings to Drought Stress and Re-watering | |
| Godase et al. | Effect of chitosan by seed priming and foliar application on growth and yield of Wal (Lablab purpureus L. Sweet) under water stress | |
| CN111316901A (en) | Application of spirulina polysaccharide and degradation product thereof in improving salt tolerance of plants | |
| Li et al. | Effect of storage, stratification, temperature and gibberellins on germination of dimorphic seeds of Suaeda salsa under saline conditions | |
| CN110754245A (en) | Preparation method and application of kelp enzymatic hydrolysate | |
| Zhang et al. | Effects of drought on physiological responses of Bretschneidera sinensis. | |
| CN106258540B (en) | A kind of process for screening and identifying of Maize Seedling salt tolerance | |
| CN111616147A (en) | Jasmonic acid methyl ester and Si4+In combination with each other |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |