CN114916557A - Agricultural composition containing lecithin and gamma-aminobutyric acid - Google Patents
Agricultural composition containing lecithin and gamma-aminobutyric acid Download PDFInfo
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- CN114916557A CN114916557A CN202210494765.XA CN202210494765A CN114916557A CN 114916557 A CN114916557 A CN 114916557A CN 202210494765 A CN202210494765 A CN 202210494765A CN 114916557 A CN114916557 A CN 114916557A
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- gamma
- aminobutyric acid
- lecithin
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- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 title claims abstract description 98
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- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 title claims abstract description 49
- 229960003692 gamma aminobutyric acid Drugs 0.000 title claims abstract description 49
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- 239000000203 mixture Substances 0.000 title claims abstract description 46
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- 244000068988 Glycine max Species 0.000 description 1
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- 241000219146 Gossypium Species 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- JEVVKJMRZMXFBT-XWDZUXABSA-N Lycophyll Natural products OC/C(=C/CC/C(=C\C=C\C(=C/C=C/C(=C\C=C\C=C(/C=C/C=C(\C=C\C=C(/CC/C=C(/CO)\C)\C)/C)\C)/C)\C)/C)/C JEVVKJMRZMXFBT-XWDZUXABSA-N 0.000 description 1
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- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
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- OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 description 1
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- 239000010452 phosphate Substances 0.000 description 1
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000008636 plant growth process Effects 0.000 description 1
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- QWDDFCKIEJCEMF-UHFFFAOYSA-N propane-1,2,3-triol 2-(trimethylazaniumyl)ethyl hydrogen phosphate Chemical compound OCC(O)CO.C[N+](C)(C)CCOP(O)([O-])=O QWDDFCKIEJCEMF-UHFFFAOYSA-N 0.000 description 1
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- ZCIHMQAPACOQHT-ZGMPDRQDSA-N trans-isorenieratene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/c1c(C)ccc(C)c1C)C=CC=C(/C)C=Cc2c(C)ccc(C)c2C ZCIHMQAPACOQHT-ZGMPDRQDSA-N 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an agricultural composition containing lecithin and gamma-aminobutyric acid. In the component A, the mass ratio of lecithin to gamma-aminobutyric acid is 12: 1-1: 15; in the agricultural composition containing lecithin and gamma-aminobutyric acid, the mass ratio of the component A to the component B in the agricultural composition is 13: 1-6: 1. Within a certain proportion range, the composition can promote the growth of crops, improve the quality of the crops, enhance the stress resistance of the crops and improve the yield of the crops.
Description
Technical Field
The invention belongs to the technical field of agricultural cultivation and planting, and relates to an agricultural composition containing lecithin and gamma-aminobutyric acid.
Background
At present, the growth regulator is mostly adopted for all green plants at home and abroad to increase the plant nutrition so as to promote the plants to grow according to the design requirements, thereby achieving the aims of increasing the yield and income. Plant growth regulating substances are divided into two categories of natural extraction and artificial synthesis, the application of the substances is one of the great progresses of modern plant physiology and agricultural technology, at present, more than one hundred products are put into market for application, and the substances become an important technical method for improving the yield and quality of agricultural products. The common plant growth regulator in the current market has the defect that certain toxic and side effects are generated when the common plant growth regulator is not used properly.
Lecithin is a phospholipid-containing substance composed of glycerol, choline, phosphoric acid and fatty acid, and is commonly present in egg yolk, animal brain, soybean, corn, cotton seed and other resources. Lecithin is composed of phosphatidylcholine or phosphocholine-glycerol base, and the triglyceride has phosphate bonded to choline. The lecithin molecule structure contains hydrophilic phosphate group and lipophilic fatty acid group, so it can be absorbed by plant to raise its disease-resisting capacity. The hydrophilic group of lecithin can keep plant leaves moist and prevent the invasion of germs, thereby inhibiting the growth and the propagation of the plant leaves. Meanwhile, lecithin also has the effect of promoting the growth of crops such as vegetables, rice and the like.
Gamma-aminobutyric acid is a four-carbon nonprotein amino acid widely existing in animals and plants, and plays an important role in nerve conduction as an inhibitory neurotransmitter in animals. Gamma-aminobutyric acid is used as a signal transduction molecule in higher plants, and plays roles in regulating stress, growth and development of the plants, storage of nitrogen and the like to the adverse environment. In the plant growth process, gamma-aminobutyric acid plays an important role, and the application of gamma-aminobutyric acid in agriculture is more and more emphasized by people.
Lentinan belongs to fungal polysaccharide, is a polysaccharide low-toxicity protective virus passivator, has remarkable control effect on virus diseases caused by tobacco mosaic virus, cucumber mosaic virus and the like, is generally applied before the virus diseases occur, can prevent crops from being infected with the virus in the growth period, and has good control effect on the fungal diseases and bacterial diseases. The related documents report that the lentinan preparation sprayed in the field can inhibit the replication of plant viruses and induce the resistance of plants, and can also promote the growth of the plants and increase the stress resistance of the plants.
Patent CN103037881B discloses a composition comprising one or more active ingredients of plant origin encapsulated with lecithin in an aqueous solution, or a synthetic analogue of said active ingredient or an extract of plant origin containing said active ingredient. Patent CN101416626A discloses a new application of gamma-aminobutyric acid, which is an application of gamma-aminobutyric acid in improving the temperature stress resistance of plants. The above two patents are respectively that the active ingredient contains lecithin and the application principle involves gamma-aminobutyric acid, but the combined use of lecithin and gamma-aminobutyric acid is not reported in the literature, and the function of the combination of lecithin and gamma-aminobutyric acid as a plant growth regulator is difficult to predict.
In the prior art, low-toxicity plant growth regulators such as forchlorfenuron and the like are generally used, and excessive use may harm human health, influence fruit quality, cause hollow fruits, reduce flavor, shorten storability and the like. The inventor of the patent application searches out an agricultural composition containing lecithin and gamma-aminobutyric acid through deep research, the composition can effectively promote the growth of crops, improve the quality of the crops, enhance the stress resistance of the crops, improve the yield of the crops, has no toxic pollution, and is suitable for mass production and long-term use. The compositions are also not found in the known literature and it is difficult for the person skilled in the art to know the beneficial effects of the compositions for plant growth regulation.
Disclosure of Invention
The invention aims to provide an agricultural composition containing lecithin and gamma-aminobutyric acid, which can effectively promote the growth of crops, improve the quality of the crops, enhance the stress resistance of the crops and improve the yield of the crops.
In view of the above objects, the present invention provides an agricultural composition comprising lecithin and gamma-aminobutyric acid, the composition comprising an effective component a and an effective component B, the component a being lecithin and gamma-aminobutyric acid, the component B being lentinan.
Further, in the agricultural composition containing lecithin and gamma-aminobutyric acid provided by the invention, in the component A, the mass ratio of the lecithin to the gamma-aminobutyric acid is 12: 1-1: 15; preferably, the mass ratio is 3: 1-1: 5.
Further, in the agricultural composition containing lecithin and gamma-aminobutyric acid provided by the invention, the mass ratio of the component A to the component B in the agricultural composition is 13: 1-6: 1; preferably, the mass ratio is 10: 1-8: 1.
According to the actual application requirement, the composition can be prepared into one of dosage forms such as an aqueous emulsion, a microemulsion, a suspending agent, wettable powder, water dispersible granules and the like according to a method known by a person skilled in the art. The functional composition also contains an auxiliary agent required by pesticide preparation, and the auxiliary agent can be one or a mixture of more of a solvent, an emulsifier, a wetting agent, a stabilizer, a dispersant, a thickening agent, a pH regulator, an antifoaming agent, an antifreezing agent, a filler and the like. The adjuvants of the present invention may be known substances, such as various adjuvants commonly used in pesticide preparations, and may be varied according to circumstances, and are not particularly limited.
The application method can adopt spraying, root irrigation, root soaking, seed soaking, throwing, broadcasting, furrow application, hole application and the like.
Compared with the prior art, the agricultural composition containing lecithin and gamma-aminobutyric acid has the following beneficial effects or advantages:
compared with the prior art, the invention mainly contributes to providing an agricultural composition containing lecithin and gamma-aminobutyric acid, wherein the active ingredients of the composition are the lecithin, the gamma-aminobutyric acid and lentinan. The inventor of the patent conducts field efficacy test on the composition, and finds that the composition promotes the growth of crops, improves the quality of the crops, enhances the stress resistance of the crops and improves the yield of the crops in a certain proportion range, and the effect is better than that of a single agent, which shows that the composition has a synergistic effect.
Detailed Description
The following examples are given to illustrate the technical means of the present invention, but the present invention is not limited to the following examples.
Example 1
This example provides a test of the agricultural composition comprising lecithin and gamma-aminobutyric acid on the promotion of seedling growth and improvement of tomato quality. The effective components of the agricultural composition are lecithin, gamma-aminobutyric acid and lentinan.
The tested tomato variety is 'New Jinfeng No. 1', and the test site is carried out in a greenhouse of gardening college of southern China university of agriculture.
Lecithin, gamma-aminobutyric acid and lentinan are compounded into a mixed treatment medicament according to the proportion shown in the table 1, and a lecithin single agent, a gamma-aminobutyric acid single agent and a lentinan single agent and clear water (CK) are respectively used as contrast treatment medicaments. The seed soaking concentration of the medicament is 200mg/L, the seed soaking time of all treatments is 24h, 4 times of repetition are set for each group of treatment, and random block arrangement is carried out among the treatments. The tomato seeds are sowed in 32-hole seedling raising plug trays after being soaked, and the culture medium is peat and perlite which are mixed in equal volume. And (3) watering clear water after sowing, starting to quantitatively irrigate complete nutrient solution (formula of Kawasaki tomatoes) in each tray after 1 week of seedling emergence, and fertilizing for 2-3 times per week.
When the tomato seedlings grow to four leaves and one heart, sampling and measuring all indexes. For each replicate, 3 plants were sampled and the plant height and stem thickness (stem base) were determined. The plants were divided into above ground and below ground (root system was cleaned) and their dry mass was measured separately. And calculating the strong seedling index, and recording the data and obtaining the result shown in table 1. After the tomato seedlings grow to four leaves and one heart, transplanting the seedlings into a planting pot, uniformly spraying a reagent to be tested once again on tomato plants in the green maturity period of the tomatoes, and treating the tomato plants with clear water as a contrast until the fruits reach commodity maturity (accord with the harvest standard). 40 fruits are randomly harvested in a cell for each treatment, the fruit is repeated for 3 times, the content of Vc, lycopene and soluble sugar is determined by adopting the prior art, and the data recording results are shown in table 1.
The strong seedling index (stem thickness/plant height + root quality/overground part dry quality) x the whole plant dry quality x 10
TABLE 1 Effect of agricultural compositions comprising lecithin and gamma-aminobutyric acid on tomato cultivation
Note: the component A is lecithin (A) 1 ) And gamma-aminobutyric acid (A) 2 ) The component B is lentinan, A 1 And A 2 The mass ratio in component A is 1: 1.
As can be seen from the data in Table 1, the agricultural composition containing lecithin and gamma-aminobutyric acid can promote the growth of tomato seedlings by using the effective components of lecithin, gamma-aminobutyric acid and lentinan within a certain ratio range, and the maximum strong seedling index is 3.81. The agricultural composition containing lecithin and gamma-aminobutyric acid has active components of lecithin, gamma-aminobutyric acid and lentinan in certain proportion range, and can raise tomato quality, and has Vc content as high as 23.10mg/100g, Fw content as high as 23.79mg/100g, soluble sugar content as high as 14.4%, and obviously higher effect than that of single preparation of the active components or the addition of lecithin and gamma-aminobutyric acid. In addition, when the fruits reach commodity maturity (growth cycle 1), the tomato yield of each group is collected, the yield of the agent treatment group is obviously higher than that of the blank control group and each single agent group, and the highest yield reaches 3322.33 kg/mu.
Example 2
This example provides a test of the agricultural composition comprising lecithin and gamma-aminobutyric acid for improving drought resistance of wheat. The effective components of the agricultural composition are lecithin, gamma-aminobutyric acid and lentinan.
The test wheat was "Yumai 22" and the test site was carried out in the greenhouse of the horticulture academy of agriculture university in south China.
As known to those skilled in the art, the biological membrane is an interface structure between plant cells and the external environment, and the effects of various stresses on the cells are first shown on the biological membrane, as are drought injuries. Under the low-temperature stress, the structure of the plasma membrane is changed, the function of the membrane is further damaged, the permeability of the cell membrane is increased, the electrolyte is leaked, the conductivity is increased, and the damage condition of the adverse environment to plants can be reflected. Furthermore, CAT enzymes can decompose H 2 O 2 Referring to CAT enzyme activity, the activity of the whole protective enzyme system of a plant can be predicted. In addition, MDA is used as a final product of membrane lipid peroxidation, can inhibit the activity of cell protective enzyme and reduce the content of antioxidant, thereby intensifying the membrane lipid peroxidation, and the MDA content can also be used as a judgment basis of the drought resistance of plants.
Lecithin, gamma-aminobutyric acid and lentinan are compounded into a mixed treatment medicament according to the proportion shown in the table 2, and a lecithin single agent, a gamma-aminobutyric acid single agent and a lentinan single agent and clear water (CK) are respectively used as contrast treatment medicaments. And after the selected wheat seeds are disinfected and pregerminated, selecting the seeds with consistent exposure, and soaking the seeds, wherein the seed soaking concentration of the medicament is 300mg/L, and the seed soaking time of all treatments is 24 h. After the seed soaking, the seeds are placed in a culture dish, and a proper amount of Hoaglands nutrient solution is added. After 15 days of culture, the water in the culture dish is sucked dry, 15ml of 20% PEG-6000 solution is added, after 48 hours of stress, the treatment agents are respectively sprayed, and 3 times of experiments are repeated. The spraying is uniform, and the spraying amount is based on the drip of the leaves. After spraying for 48h, seedling leaves are randomly selected in the plot for each treatment, various physiological indexes are determined by adopting the prior art, and the data recording results are shown in table 2.
TABLE 2 Effect of agricultural compositions comprising lecithin and gamma-aminobutyric acid on drought resistance of wheat
Note: the component A is lecithin (A) 1 ) And gamma-aminobutyric acid (A) 2 ) The component B is lentinan, A 1 And A 2 The mass ratio in component A is 1: 1.
The results in Table 2 show that the agricultural composition comprising lecithin and gamma-aminobutyric acid has a higher CAT activity, up to 49.78 mg/g.min, after treatment compared to the blank control; the MDA content is low, and the minimum content is 70.13 mu mol/g-Fw; the relative water content of the leaves is high and is 74.43% at the highest, which shows that the test medicaments have the function of improving the cold resistance of wheat seedlings. Meanwhile, compared with the use of a single agent, the compound use effect of the lecithin and the gamma-aminobutyric acid is better, and the compound use of the lecithin and the gamma-aminobutyric acid has a synergistic effect.
As described above, the present invention can be preferably implemented, and the above-mentioned embodiments only describe the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various changes and modifications of the technical solution of the present invention made by those skilled in the art without departing from the design spirit of the present invention shall fall within the protection scope defined by the present invention.
Claims (7)
1. An agricultural composition containing lecithin and gamma-aminobutyric acid, wherein the effective components comprise a component A, and the component A is the lecithin and the gamma-aminobutyric acid.
2. The agricultural composition of claim 1, wherein in the component A, the mass ratio of the lecithin to the gamma-aminobutyric acid is 12: 1-1: 15.
3. The agricultural composition of claim 2, wherein in the component A, the mass ratio of the lecithin to the gamma-aminobutyric acid is 3: 1-1: 5.
4. An agricultural composition according to claim 1, characterized in that the composition further comprises component B, which is lentinan.
5. The agricultural composition of claim 1, wherein the mass ratio of the component A to the component B in the agricultural composition is 13: 1-6: 1.
6. The agricultural composition as claimed in claim 5, wherein the mass ratio of the component A to the component B in the agricultural composition is 10: 1-8: 1.
7. The agricultural composition of any one of claims 1 to 6 for promoting the growth of crops, improving the quality of crops, enhancing the stress resistance of crops and increasing the yield of crops.
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WO2009114959A1 (en) * | 2008-03-20 | 2009-09-24 | 中国人民解放军军事医学科学院毒物药物研究所 | Injectalble sustained-release pharmaceutical formulation and method for preparing it |
CN103535380A (en) * | 2013-10-28 | 2014-01-29 | 成都新朝阳作物科学有限公司 | Crop drought resistant agent as well as application thereof |
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CN113100251A (en) * | 2021-04-25 | 2021-07-13 | 渭南东旺农华生物科技有限公司 | Agricultural composition containing lecithin |
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