CN103356767B - Medicinal composition for treating bacterial diarrhea of livestock as well as preparation method and application thereof - Google Patents
Medicinal composition for treating bacterial diarrhea of livestock as well as preparation method and application thereof Download PDFInfo
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- CN103356767B CN103356767B CN201310344409.0A CN201310344409A CN103356767B CN 103356767 B CN103356767 B CN 103356767B CN 201310344409 A CN201310344409 A CN 201310344409A CN 103356767 B CN103356767 B CN 103356767B
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Abstract
The invention provides a medicinal composition for treating the bacterial diarrhea of livestock. The medicinal composition is prepared from the following active medicinal components in parts by weight: 15-30 parts of potentilla chinensis and 15 parts of flos hibisci. The medicinal composition with the optimum effect on treating the bacterial diarrhea of livestock is finally prepared by selecting the Chinese medicinal herbs and determining the amount of the Chinese medicinal herbs. The medicinal composition provided by the invention is effective to more than 95% of a variety of diseases and can serve as a novel medicine for clinical treatment.
Description
Technical field
The present invention relates to a kind of veterinary medical composition for the treatment of bacterial diarrhea and its production and use.
Background technology
Diarrhoea is a total symptom of various diseases, according to the difference of the cause of disease, pathogenicity can be divided into suffer from diarrhoea (bacterial diarrhea, spirochetal diarrhoea, viral diarrhea, parasitic diarrhea) for diarrhoea and avirulence diarrhoea (lienteric diarrhea, dystrophic diarrhoea, allergic diarrhea) two large classes.
The common cause of disease of bacterial diarrhea has escherichia coli, Salmonella, can cause yellow and white dysentery of piglet, chicken colibacillosis and baby swine paratyphoid.Yellow scour of piglet is often sent out within 3 ages in days, high and draw yellow loose stool for principal character with mortality rate; Piglet pujos blancos is often sent out in 14 ~ 21 ages in days, with draw milky or canescence with bad smell loose stool, the course of disease is long, mortality rate is low for principal character; The cardinal symptom of sick chicken is row's yellow, white, canescence, light green or yellow green light stool, and perianal fine hair is stained with in dirt, is multiplely born in chickling, and mortality rate is high.The diarrhoea that paratyphoid fever causes often sends out the ablactational baby pig more than 30 ages in days, and acutely to suffer from diarrhoea, high heat, skin occurs that purpura, purulent conjunctivitis, septicemia death are principal character.Medicine and vaccine is mainly adopted to prevent and treat at present.
Livestock and poultry diarrhea is always a great problem of animal cultivation, veterinary and raiser find out all ways and solve this problem, but often effect is unsatisfactory, and antibiotic abuse directly results in the generation of series of side effects, therefore in conjunction with Chinese veterinarian's theory, seek a kind of efficient, green, simple herbal medicine and be imminent.
Summary of the invention
The object of the present invention is to provide a kind of veterinary medical composition for the treatment of bacterial diarrhea and its production and use.
The invention provides a kind of veterinary medical composition for the treatment of bacterial diarrhea, it is the preparation be prepared from by the crude drug of following weight proportion: Herba Potentillae Chinensis 15 ~ 30 parts of Flos Hibisci 15 parts.
Further, it is the preparation be prepared from by the crude drug of following weight proportion: Herba Potentillae Chinensis 30 parts of Flos Hibisci 15 parts.
Wherein, described Herba Potentillae Chinensis is the dry herb of rosaceous plant Herba Potentillae Chinensis Potentilla chinensis Ser.; Flos Hibisci is Malvaceae plant Hibiscus syriacus L. Hibiscus syriacus L. or the dried floral spending polyphyll Hibiscus syriacus L. Hibiscussyriacus L.f.albusplenus London in vain.
Wherein, described preparation is oral agents.
Further, the preparation method of described preparation comprises following operating procedure:
(1) weighting raw materials by weight ratio;
(2) after getting crude drug decocting, adding ethanol to alcohol content in decocting liquid is 70%v/v, leaves standstill, gets supernatant, after reclaiming ethanol, is prepared into veterinary formulations.
Further, in step (2), before adding ethanol, first decocting liquid being concentrated into 50 DEG C, to record relative density be 1.14 ~ 1.16.
Present invention also offers the preparation method of above-mentioned veterinary medical composition, it comprises following operating procedure:
(1) weighting raw materials by weight ratio;
(2) after getting crude drug decocting, adding ethanol to alcohol content in decocting liquid is 70%v/v, leaves standstill, gets supernatant, after reclaiming ethanol, is prepared into veterinary formulations.
Further, in step (2), before adding ethanol, first decocting liquid being concentrated into 50 DEG C, to record relative density be 1.14 ~ 1.16.
Present invention also offers the purposes of above-mentioned veterinary medical composition in the medicine of preparation treatment poultry bacterial diarrhea.
Further, described bacterial diarrhea is the diarrhoea caused by Salmonella or escherichia coli.
Further, described medicine is the medicine for the treatment of HUANGBAI(sic) dysentery, baby swine paratyphoid or chicken colibacillosis.
Present invention also offers above-mentioned veterinary medical composition and prepare purposes in anti-salmonella or colibacillary medicine.
The present invention, by screening to medical material consumption, finally obtains the pharmaceutical composition to poultry bacterial diarrhea curative effect the best, to the effective percentage of various diseases up to more than 95%, for clinical application provides new selection.
Detailed description of the invention
The preparation method of embodiment 1 pharmaceutical composition of the present invention
Get Herba Potentillae Chinensis 300g, Flos Hibisci 150g, soak about half an hour, then decoct secondary, 1 hour first time, for the second time half an hour, collecting decoction, filters, it is about 1.14 ~ 1.16 (50 DEG C) that filtrate is concentrated into relative density, and adding ethanol to alcohol content is 70%v/v, abundant mix homogeneously, leave standstill cold preservation 24 hours, filter, filtrate recycling ethanol, concentrated volume is original 5%, adds appropriate Icing Sugar and dextrin mixing, granulates, 60 DEG C of oven dry, make 300g, obtain granule.
The Herba Potentillae Chinensis used in the embodiment of the present invention derives from the dry herb of rosaceous plant Herba Potentillae Chinensis Potentillachinensis Ser.; Flos Hibisci is Malvaceae plant Hibiscus syriacus L. Hibiscus syriacus L. or the dried floral spending polyphyll Hibiscus syriacus L. Hibiscus syriacus L.f.albusplenus London in vain.
The preparation method of embodiment 2 pharmaceutical composition of the present invention
Get Herba Potentillae Chinensis 300g, Flos Hibisci 150g, soak about half an hour, then secondary is decocted, 1 hour first time, half an hour for the second time, collecting decoction, filter, it is 1.14 ~ 1.16(50 DEG C that filtrate is concentrated into relative density), adding ethanol to alcohol content is 70%v/v, abundant mix homogeneously, leave standstill cold preservation 24 hours, filter, decompression recycling ethanol is extremely without alcohol taste, cold preservation 12 hours, filter, filtrate adds water in right amount, adjust pH to 7, stir evenly, cold preservation 24 hours, filter, filtrate adjust pH to 7, add water and make 300ml, stir evenly, leave standstill 12 hours, filter, fill, sterilizing, obtain oral liquid.
Beneficial effect of the present invention is illustrated below by way of test example.
Test example 1 antibacterial effect is studied
1 material
1.1 medical material
Herba Potentillae Chinensis Flos Hibisci
1.2 key instrument
RE-52CS type Rotary Evaporators, the sub-flourish biochemical instrument instrument factory in Shanghai; UV-1800-PC type ultraviolet spectrophotometer, Shanghai Mei Puda Instrument Ltd..
1.3 strain and reagent
Escherichia coli CMCC (B) 44102, Salmonella CMCC (B) 50094, national DSMZ.Nutrient broth medium, Qingdao GaoKeYuan Hai Bo Bioisystech Co., Ltd.
2 methods
Respectively by Herba Potentillae Chinensis: Flos Hibisci (30g:15g) takes 5 parts of crude drugs, decocts with water twice respectively, 1 hour first time, for the second time half an hour, collecting decoction, filter, filtrate is concentrated into relative density 1.14 ~ 1.16(50 DEG C);
A copy of it regulates concentration to being equivalent to crude drug in whole concentration 1g/mL, and adjust pH 7 is for subsequent use.
Other 4 parts, add ethanol respectively and make alcohol content reach 60%, 70%, 80%, 90%, abundant mix homogeneously, leave standstill cold preservation 24 hours, filter, decompression recycling ethanol is extremely without alcohol taste, and filtrate adds water in right amount, is equivalent to crude drug in whole concentration 1g/mL, and adjust pH 7 is for subsequent use.
Adopt test tube doubling dilution: get sterilizing test tubes 9 and number, be arranged on test tube rack by the numbering of l-9, nutrient broth 0.5mL is added respectively by sterile working's requirement, then draw extracting solution 0.5mL respectively and be placed in l pipe, draw 0.5mL from the 1st pipe after mixing and add the 2nd pipe, the rear 0.5mL of absorption of same mixing is put people the 3rd and is managed, and method is diluted to the 8th pipe by pipe according to this, 8th pipe reject 0.5mL, the 9th pipe does not add extracting solution as positive control.Test tube observing bacterial growth situation is taken out after 37 DEG C of cultivation 12h.MIC criterion: after 37 DEG C of cultivation 12h, often in vitro draw 100 μ L liquid microplate reader and measure OD values, and respectively manage interior bacterial growth situation in conjunction with perusal.
3 results
The results are shown in Table 1.
The different alcohol precipitation concentration of table 1 is to the MIC result of different bacterium
Can draw thus, adopt the precipitate with ethanol extract of concentration 70%, can significantly improve the antibacterial action of Herba Potentillae Chinensis and Flos Hibisci compound recipe, its antibacterial activity is significantly better than the precipitate with ethanol extract of decocting liquid and other concentration.
Test example 2 clinical trial
1 material
HUANGBAI(sic) dysentery piglet 20, paratyphoid fever piglet 20, the sick chicken 50 of colibacillosis; Herba Potentillae Chinensis Flos Hibisci oral liquid (prepared by embodiment 2), injection liquid of gentamicin, the former powder of norfloxacin.
2 methods
2.1 administering mode
Oral liquid treatment group: HUANGBAI(sic) dysentery piglet is filled with according to the dosage of 10ml/ head/d and feeds, and is used in conjunction 3d.Paratyphoid fever piglet, with the dosage gavage of 20ml/ head/d, is used in conjunction 3d.Colibacillosis chicken, with 2ml//d drinking-water, is used in conjunction 3d; Western medicine group: gentamycin (80,000 IU/ prop up) every sow 5/time, 2 times/d injects, and is used in conjunction 3d.Norfloxacin former every, powder chicken 2mg/ time, 1 time/d, is used in conjunction 3d.
2.2 efficacy determination
Cure: dysentery disappears, feces recovers normal.Effective: except cure be effective except, dysentery alleviates, and feces take a favorable turn.Invalid: dysentery symptom nothing after treatment takes a turn for the better or increases the weight of even dead.
3 results
The results are shown in Table 2.
Table 2 clinical test results
Result of the test shows, pharmaceutical composition of the present invention to yellow and white dysentery of piglet and baby swine paratyphoid therapeutical effect clearly, effective percentage more than 95%, to chicken colibacillosis effective percentage more than 96%.
In sum, the present invention, by screening to medical material consumption, finally obtains the pharmaceutical composition to poultry bacterial diarrhea curative effect the best, to the effective percentage of various diseases up to more than 95%, for clinical application provides new selection.
Claims (9)
1. treat a veterinary medical composition for bacterial diarrhea, it is characterized in that: it is the preparation be prepared from by the crude drug of following weight proportion:
Herba Potentillae Chinensis 30 parts of Flos Hibisci 15 parts;
Described preparation adopts following operating procedure to prepare:
(1) weighting raw materials by weight ratio;
(2) after getting crude drug decocting, adding ethanol to alcohol content in decocting liquid is 70%v/v, leaves standstill, gets supernatant, after reclaiming ethanol, is prepared into veterinary formulations.
2. veterinary medical composition according to claim 1, is characterized in that: described Herba Potentillae Chinensis is the dry herb of rosaceous plant Herba Potentillae Chinensis Potentilla chinensis Ser.; Flos Hibisci is Malvaceae plant Hibiscus syriacus L. Hibiscus syriacus L. or the dried floral spending polyphyll Hibiscus syriacus L. Hibiscus syriacus L.f.albusplenusLondon in vain.
3. veterinary medical composition according to claim 1 and 2, is characterized in that: described preparation is oral agents.
4. the preparation method of veterinary medical composition described in claims 1 to 3 any one, is characterized in that: it comprises following operating procedure:
(1) weighting raw materials by weight ratio;
(2) after getting crude drug decocting, adding ethanol to alcohol content in decocting liquid is 70%v/v, leaves standstill, gets supernatant, after reclaiming ethanol, is prepared into veterinary formulations.
5. preparation method according to claim 4, is characterized in that: in step (2), before adding ethanol, and first decocting liquid being concentrated into 50 DEG C, to record relative density be 1.14 ~ 1.16.
6. the purposes of veterinary medical composition described in claims 1 to 3 any one in the medicine of preparation treatment poultry bacterial diarrhea.
7. purposes according to claim 6, is characterized in that: described bacterial diarrhea is the diarrhoea caused by Salmonella or escherichia coli.
8. purposes according to claim 6, is characterized in that: described medicine is the medicine for the treatment of HUANGBAI(sic) dysentery, baby swine paratyphoid or chicken colibacillosis.
9. veterinary medical composition described in claims 1 to 3 any one is preparing purposes in anti-salmonella or colibacillary medicine.
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| CN105106269A (en) * | 2015-09-17 | 2015-12-02 | 成都乾坤动物药业有限公司 | Traditional Chinese veterinary medicine for treating livestock and poultry bacterial diarrhea, preparation method and application thereof |
| CN105287683A (en) * | 2015-12-01 | 2016-02-03 | 蓬溪发联农牧有限公司 | Pharmaceutical composition for preventing and treating flatulence and diarrhea of rabbit and preparation and application of pharmaceutical composition |
| KR102459434B1 (en) * | 2020-07-28 | 2022-10-25 | 한국한의약진흥원 | Composition for improvment, prevention or treatment in muscular atrophy comprising extracts of Potentilla chinensis or Indigo |
| CN113082088B (en) * | 2021-04-01 | 2022-06-21 | 中国农业科学院兰州畜牧与兽药研究所 | Polygonum capitatum extract preparation for treating piglet diarrhea for livestock and preparation method and application thereof |
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| 倾尘梦雨.百度百科-委陵菜.《http://baike.***.com/history/46485130》.2013,1-5. * |
| 几种常见药用花卉;计皇成;《中国花卉盆景》;19980531(第05期);16 * |
| 白头木槿汤治牛痢疾43例;孙武;《中兽医医药杂志》;19851231(第02期);51 * |
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Address after: 611130 Wenjiang, Chengdu, Chengdu cross strait science and Technology Industrial Development Zone, Jin Fu Road, Sichuan Patentee after: Chengdu Qiankun animal pharmaceutical Limited by Share Ltd Address before: 610000, Chengdu District, Sichuan City, Wenjiang province Chengdu science and Technology Industrial Development Zone on both sides of the road, Golden House Road Patentee before: Chengdu Qiankun Animal Pharmaceutical Co.,Ltd. |