CN103356767A - Medicinal composition for treating bacterial diarrhea of livestock as well as preparation method and application thereof - Google Patents
Medicinal composition for treating bacterial diarrhea of livestock as well as preparation method and application thereof Download PDFInfo
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- CN103356767A CN103356767A CN2013103444090A CN201310344409A CN103356767A CN 103356767 A CN103356767 A CN 103356767A CN 2013103444090 A CN2013103444090 A CN 2013103444090A CN 201310344409 A CN201310344409 A CN 201310344409A CN 103356767 A CN103356767 A CN 103356767A
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Abstract
The invention provides a medicinal composition for treating the bacterial diarrhea of livestock. The medicinal composition is prepared from the following active medicinal components in parts by weight: 15-30 parts of potentilla chinensis and 15 parts of flos hibisci. The medicinal composition with the optimum effect on treating the bacterial diarrhea of livestock is finally prepared by selecting the Chinese medicinal herbs and determining the amount of the Chinese medicinal herbs. The medicinal composition provided by the invention is effective to more than 95% of a variety of diseases and can serve as a novel medicine for clinical treatment.
Description
Technical field
The present invention relates to a kind of veterinary drug composition and method of making the same and purposes for the treatment of bacterial diarrhea.
Background technology
Diarrhoea is a total symptom of various diseases, according to the difference of the cause of disease, diarrhoea can be divided into pathogenicity diarrhoea (bacterial diarrhea, spirillum diarrhoea, viral diarrhea, parasitic diarrhea) and avirulence diarrhoea (lienteric diarrhea, dystrophic diarrhoea, allergic diarrhea) two large classes.
The common cause of disease of bacterial diarrhea has escherichia coli, Salmonella, can cause yellow and white dysentery of piglet, chicken colibacillosis and baby swine paratyphoid.Yellow scour of piglet is often sent out in 3 ages in days with interior, and is high and draw yellow loose stool as principal character take mortality rate; Piglet pujos blancos is often sent out in 14～21 ages in days, and is low as principal character with loose stool, course of disease length, the mortality rate of stench flavor to draw milky or canescence; The cardinal symptom of sick chicken is row's yellow, white, canescence, light green or yellow green light stool, and perianal fine hair is stained with in dirt, multiplely is born in chickling, and mortality rate is high.The diarrhoea that paratyphoid fever causes is often sent out the ablactational baby pig more than 30 ages in days, purpura, purulent conjunctivitis, septicemia death occur as principal character take violent diarrhoea, high heat, skin.Mainly be to adopt medicine and vaccine to prevent and treat at present.
Livestock and poultry diarrhea always is a great problem of animal cultivation, veterinary and raiser find out all ways and address this problem, but often effect is unsatisfactory, and antibiotic abuse has directly caused the generation of series of side effects, therefore theoretical in conjunction with Chinese veterinarian, seek a kind of efficient, green, simple herbal medicine and be imminent.
Summary of the invention
The object of the present invention is to provide a kind of veterinary drug composition and method of making the same and purposes for the treatment of bacterial diarrhea.
The invention provides a kind of veterinary drug compositions for the treatment of bacterial diarrhea, it is the preparation that the crude drug by following weight proportion is prepared from: 15 parts of 15～30 parts of Flos Hibisci of Herba Potentillae Chinensis.
Further, it is the preparation that the crude drug by following weight proportion is prepared from: 15 parts of 30 parts of Flos Hibisci of Herba Potentillae Chinensis.
Wherein, described Herba Potentillae Chinensis is the dry herb of rosaceous plant Herba Potentillae Chinensis Potentilla chinensis Ser.; Flos Hibisci is Malvaceae plant Hibiscus syriacus L. Hibiscus syriacus L. or the dried floral of spending polyphyll Hibiscus syriacus L. Hibiscus syriacus L.f.albusplenus London in vain.
Wherein, described preparation is oral agents.
Further, the preparation method of described preparation comprises following operating procedure:
(1) weighting raw materials by weight ratio;
(2) get the crude drug decocting after, add ethanol in the decocting liquid and be 70%v/v to containing alcohol amount, leave standstill, get supernatant, behind the Recycled ethanol, be prepared into veterinary formulations.
Further, in the step (2), add ethanol before, first decocting liquid being concentrated into 50 ℃, to record relative density be 1.14～1.16.
The present invention also provides the preparation method of above-mentioned veterinary drug compositions, and it comprises following operating procedure:
(1) weighting raw materials by weight ratio;
(2) get the crude drug decocting after, add ethanol in the decocting liquid and be 70%v/v to containing alcohol amount, leave standstill, get supernatant, behind the Recycled ethanol, be prepared into veterinary formulations.
Further, in the step (2), add ethanol before, first decocting liquid being concentrated into 50 ℃, to record relative density be 1.14～1.16.
The present invention also provides the purposes of above-mentioned veterinary drug compositions in the medicine of preparation treatment fowl poultry bacterial diarrhea.
Further, described bacterial diarrhea is by the diarrhoea due to Salmonella or the escherichia coli.
Further, described medicine is the medicine for the treatment of HUANGBAI(sic) dysentery, baby swine paratyphoid or chicken colibacillosis.
The present invention also provides above-mentioned veterinary drug compositions purposes in preparation anti-salmonella or colibacillary medicine.
The present invention has finally obtained the pharmaceutical composition to fowl poultry bacterial diarrhea curative effect the best by to the screening of medical material consumption, to the effective percentage of various diseases up to more than 95%, for clinical application provides new selection.
The specific embodiment
The preparation method of embodiment 1 pharmaceutical composition of the present invention
Get Herba Potentillae Chinensis 300g, Flos Hibisci 150g soaks about half an hour, then decocts secondary, 1 hour for the first time, for the second time half an hour, collecting decoction filtered, it is about 1.14～1.16 (50 ℃) that filtrate is concentrated into relative density, adds ethanol and is 70%v/v, fully mix homogeneously to containing the alcohol amount, leave standstill cold preservation 24 hours, and filtered filtrate recycling ethanol, concentrated volume is original 5%, adds an amount of Icing Sugar and dextrin mixing, granulates, 300g is made in 60 ℃ of oven dry, namely gets granule.
The Herba Potentillae Chinensis that uses in the embodiment of the invention derives from the dry herb of rosaceous plant Herba Potentillae Chinensis Potentilla chinensis Ser.; Flos Hibisci is Malvaceae plant Hibiscus syriacus L. Hibiscus syriacus L. or the dried floral of spending polyphyll Hibiscus syriacus L. Hibiscus syriacus L.f.albusplenus London in vain.
The preparation method of embodiment 2 pharmaceutical compositions of the present invention
Get Herba Potentillae Chinensis 300g, Flos Hibisci 150g soaks about half an hour, then decoct secondary, 1 hour for the first time, the half an hour second time, collecting decoction filters, and it is 1.14～1.16(50 ℃ that filtrate is concentrated into relative density), add ethanol and be 70%v/v to containing the alcohol amount, fully mix homogeneously left standstill cold preservation 24 hours, filter, decompression recycling ethanol is to distinguish the flavor of cold preservation 12 hours without alcohol, filter, it is an amount of that filtrate adds water, adjust pH to 7, stir evenly, cold preservation 24 hours filters, filtrate adjust pH to 7 adds water and makes 300ml, stirs evenly, left standstill 12 hours, and filtered fill, sterilization namely gets oral liquid.
Below specify beneficial effect of the present invention by test example.
The research of test example 1 antibacterial effect
1 material
1.1 medical material
The Herba Potentillae Chinensis Flos Hibisci
1.2 key instrument
RE-52CS type Rotary Evaporators, the inferior flourish biochemical instrument instrument factory in Shanghai; UV-1800-PC type ultraviolet spectrophotometer, the U.S. spectrum in Shanghai reaches Instr Ltd..
1.3 strain and reagent
Escherichia coli CMCC (B) 44102, Salmonella CMCC (B) 50094, national DSMZ.Nutrient broth medium, Qingdao GaoKeYuan Hai Bo Bioisystech Co., Ltd.
2 methods
Respectively by Herba Potentillae Chinensis: Flos Hibisci (30g:15g) takes by weighing 5 parts of crude drugs, decocts with water respectively twice, and 1 hour for the first time, for the second time half an hour, collecting decoction filtered, and filtrate is concentrated into relative density 1.14～1.16(50 ℃);
A copy of it is regulated concentration to being equivalent to crude drug in whole concentration 1g/mL, and adjust pH 7 is for subsequent use.
Other 4 parts, add respectively ethanol and make and contain alcohol amount and reach 60%, 70%, 80%, 90%, fully mix homogeneously left standstill cold preservation 24 hours, filtered, and decompression recycling ethanol is to without the alcohol flavor, and it is an amount of that filtrate adds water, is equivalent to crude drug in whole concentration 1g/mL, and adjust pH 7 is for subsequent use.
Adopt the test tube doubling dilution: get 9 of sterilizing test tubes and numbering, numbering by l-9 is arranged on the test tube rack, add respectively nutrient broth 0.5mL by sterile working's requirement, then draw respectively extracting solution 0.5mL and place the l pipe, draw 0.5mL from the 1st pipe behind the mixing and add the 2nd pipe, draw 0.5mL behind the same mixing and put people's the 3rd pipe, method is diluted to the 8th pipe by pipe according to this, the 8th pipe reject 0.5mL, the 9th pipe does not add extracting solution as positive control.Take out test tube observing bacterial growth situation behind 37 ℃ of cultivation 12h.MIC criterion: behind 37 ℃ of cultivation 12h, whenever in vitro draw 100 μ L liquid and measure the OD value with microplate reader, and in conjunction with bacterial growth situation in each pipe of perusal.
3 results
The results are shown in Table 1.
The different alcohol precipitation concentrations of table 1 are to the MIC result of different bacterium
Can draw thus, adopt the precipitate with ethanol extract of concentration 70%, can significantly improve the antibacterial action of Herba Potentillae Chinensis and Flos Hibisci compound recipe, its antibacterial activity significantly is better than the precipitate with ethanol extract of decocting liquid and other concentration.
Test example 2 clinical trials
1 material
20 of HUANGBAI(sic) dysentery piglets, 20 of paratyphoid fever piglets, 50 of the sick chickens of colibacillosis; Herba Potentillae Chinensis Flos Hibisci oral liquid (embodiment 2 preparations), injection liquid of gentamicin, the former powder of norfloxacin.
2 methods
2.1 administering mode
The oral liquid treatment group: HUANGBAI(sic) dysentery piglet is filled with according to the dosage of 10ml/ head/d and feeds, and is used in conjunction 3d.The paratyphoid fever piglet is used in conjunction 3d with the dosage gavage of 20ml/ head/d.The colibacillosis chicken is used in conjunction 3d with 2ml//d drinking-water; The Western medicine group: 5/time of every sows of gentamycin (80,000 IU/ prop up), 2 times/d injection is used in conjunction 3d.Every chicken of the former powder of norfloxacin 2mg/ time 1 time/d, is used in conjunction 3d.
2.2 efficacy determination
Cure: dysentery disappears, and it is normal that feces recovers.Effectively: except cure be effective, dysentery alleviates, feces take a favorable turn.Invalid: dysentery symptom nothing after treatment takes a turn for the better or increases the weight of even death.
3 results
The results are shown in Table 2.
Table 2 clinical test results
Result of the test shows, pharmaceutical composition of the present invention to yellow and white dysentery of piglet and baby swine paratyphoid therapeutical effect clearly, effective percentage is more than 95%, to the chicken colibacillosis effective percentage more than 96%.
In sum, the present invention has finally obtained the pharmaceutical composition to fowl poultry bacterial diarrhea curative effect the best by to the screening of medical material consumption, to the effective percentage of various diseases up to more than 95%, for clinical application provides new selection.
Claims (10)
1. veterinary drug compositions for the treatment of bacterial diarrhea is characterized in that: it is the preparation that the crude drug by following weight proportion is prepared from:
15 parts of 15～30 parts of Flos Hibisci of Herba Potentillae Chinensis.
2. veterinary drug compositions according to claim 1 is characterized in that: it is the preparation that the crude drug by following weight proportion is prepared from:
15 parts of 30 parts of Flos Hibisci of Herba Potentillae Chinensis.
3. veterinary drug compositions according to claim 1 and 2, it is characterized in that: described Herba Potentillae Chinensis is the dry herb of rosaceous plant Herba Potentillae Chinensis Potentilla chinensis Ser.; Flos Hibisci is Malvaceae plant Hibiscus syriacus L. Hibiscus syriacus L. or the dried floral of spending polyphyll Hibiscus syriacus L. Hibiscus syriacus L.f.albusplenus London in vain.
4. the described veterinary drug compositions of any one according to claim 1～3, it is characterized in that: described preparation is oral agents.
5. the preparation method of the described veterinary drug compositions of claim 1～4 any one, it is characterized in that: it comprises following operating procedure:
(1) weighting raw materials by weight ratio;
(2) get the crude drug decocting after, add ethanol in the decocting liquid and be 70%v/v to containing alcohol amount, leave standstill, get supernatant, behind the Recycled ethanol, be prepared into veterinary formulations.
6. preparation method according to claim 5 is characterized in that: in the step (2), add ethanol before, first decocting liquid being concentrated into 50 ℃, to record relative density be 1.14～1.16.
7. the purposes of the described veterinary drug compositions of claim 1～4 any one in the medicine of preparation treatment fowl poultry bacterial diarrhea.
8. purposes according to claim 7, it is characterized in that: described bacterial diarrhea is by the diarrhoea due to Salmonella or the escherichia coli.
9. purposes according to claim 7 is characterized in that: described medicine is the medicine for the treatment of HUANGBAI(sic) dysentery, baby swine paratyphoid or chicken colibacillosis.
10. the described veterinary drug compositions of claim 1～4 any one purposes in preparation anti-salmonella or colibacillary medicine.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105106269A (en) * | 2015-09-17 | 2015-12-02 | 成都乾坤动物药业有限公司 | Traditional Chinese veterinary medicine for treating livestock and poultry bacterial diarrhea, preparation method and application thereof |
| US20180256661A1 (en) * | 2015-12-01 | 2018-09-13 | Sichuan Xingzhi Zhihui Intellectual Property Operation Co., Ltd. | Pharmaceutical Composition for Prevention and Treatment of Flatulence and Diarrhea in Rabbit and Formula and Application Thereof |
| CN113082088A (en) * | 2021-04-01 | 2021-07-09 | 中国农业科学院兰州畜牧与兽药研究所 | Polygonum capitatum extract preparation for treating piglet diarrhea for livestock and preparation method and application thereof |
| CN113995133A (en) * | 2020-07-28 | 2022-02-01 | 韩国韩医药振兴院 | Composition for improving, preventing or treating muscular atrophy comprising Potentilla tormentilla extract or Polygonum tinctorium extract |
-
2013
- 2013-08-08 CN CN201310344409.0A patent/CN103356767B/en active Active
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105106269A (en) * | 2015-09-17 | 2015-12-02 | 成都乾坤动物药业有限公司 | Traditional Chinese veterinary medicine for treating livestock and poultry bacterial diarrhea, preparation method and application thereof |
| US20180256661A1 (en) * | 2015-12-01 | 2018-09-13 | Sichuan Xingzhi Zhihui Intellectual Property Operation Co., Ltd. | Pharmaceutical Composition for Prevention and Treatment of Flatulence and Diarrhea in Rabbit and Formula and Application Thereof |
| CN113995133A (en) * | 2020-07-28 | 2022-02-01 | 韩国韩医药振兴院 | Composition for improving, preventing or treating muscular atrophy comprising Potentilla tormentilla extract or Polygonum tinctorium extract |
| CN113082088A (en) * | 2021-04-01 | 2021-07-09 | 中国农业科学院兰州畜牧与兽药研究所 | Polygonum capitatum extract preparation for treating piglet diarrhea for livestock and preparation method and application thereof |
| CN113082088B (en) * | 2021-04-01 | 2022-06-21 | 中国农业科学院兰州畜牧与兽药研究所 | Polygonum capitatum extract preparation for treating piglet diarrhea for livestock and preparation method and application thereof |
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| CN103356767B (en) | 2015-07-22 |
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Address after: 611130 Wenjiang, Chengdu, Chengdu cross strait science and Technology Industrial Development Zone, Jin Fu Road, Sichuan Patentee after: Chengdu Qiankun animal pharmaceutical Limited by Share Ltd Address before: 610000, Chengdu District, Sichuan City, Wenjiang province Chengdu science and Technology Industrial Development Zone on both sides of the road, Golden House Road Patentee before: Chengdu Qiankun Animal Pharmaceutical Co.,Ltd. |