CN103026988A - Indoor intensive artificial breeding technique of Yangtze River Coilia ectenes - Google Patents
Indoor intensive artificial breeding technique of Yangtze River Coilia ectenes Download PDFInfo
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Abstract
The invention relates to an indoor intensive artificial breeding technique of Yangtze River Coilia ectenes, which comprises the steps of Coilia ectenes parent fish enhanced breeding, artificial spawning induction, artificial hatching and fingerling breeding. The indoor intensive artificial breeding technique of Yangtze River Coilia ectenes is characterized in that Coilia ectenes adult fish which is alternately cultured for three years by using seawater and freshwater is used as parent fish for the Coilia ectenes parent fish enhanced breeding, female parent fish individuals with clearly visible ovary outlines and softer inferior bellies and male parent fish individuals with milk white semen which flows out when the bellies are slightly pressed are selected for the artificial spawning induction, the female-male ratio is 1:3, the female parent fish and the male parent fish naturally mate for laying eggs and fertilization, temperature-controlled inflated hatching in still water is conducted to fertilized eggs, and the fingerling breeding comprises culture at four stages, wherein the culture at the first stage comprises the steps of temporarily culturing newly hatched fries from the time that fries are newly hatched to the time that the fries open mouths, the culture at the second stage comprises the step of culturing the fingerlings from body length being 3mm to body length being 20mm, the culture at the third stage comprises the step of culturing the fingerlings from body length being 20mm to body length being 30mm, the culture at the fourth stage comprises the step of culturing the fingerlings from body length being 30mm to body length being 40-50mm, and the fingerlings can be put in a pond for conventional culture after the body length of the fingerlings reaches 40-50mm.
Description
Technical field
The present invention relates to the migration fishes artificial propagation techniques, especially the indoor intensive artificial breeding technique of Coilia ectenes in Yangtze River.
Background technology
Cutter long-tailed anchovy (Coilia ectenes) belongs to Clupeiformes, Anchovy section, long-tailed anchovy genus, is commonly called as hairtail, rough cutter fish, Mao Huayu, beard fish, tapertail anchovy etc.Mainly be distributed in the yellow Bohai Sea of China and the East Sea one band, all maritime rivers such as the Liaohe River, Haihe River, the Yellow River, the Changjiang river, the Qiantang River all can be seen, and it is with fine and tender taste, and delicious flavour and being celebrated is the famous and precious migrating fish class of enjoying one of " the Changjiang river three delicacies " good reputation.In recent years, because the impact of the factors such as overfishing and the deterioration of the ecological environment, cutter long-tailed anchovy resource sharply failed, and the market demand is growing on and on, even to the awkward situation that has price but no buyers, clear and bright front and back in 2011, the cutter long-tailed anchovy market price of three liang of left and right sides specifications is up to 7000 yuan/Kg.Although at present more domestic research institutions and production unit seeking always a kind of be similar to four large Chinese carps pass through catch ripe wild female, male parent population and carry out the artificial insemination mode and grow seedlings, yet suffer defeat after defeat, there is no example successfully.Also there are some units to carry out such as filling with Jiang Namiao, drawing in the net to catch seedling along the Yangtze River and carry out culture experiment, idol has indivedual reports of cutter long-tailed anchovy artificial propagation success, the success of palette knife long-tailed anchovy artificial propagation exists great contingency, the artificial propagation of cutter long-tailed anchovy also has a lot of technical parameters fuzzyyer, more there is contingency in seed rearing, mostly adopt pond of opening prelarva to put in a suitable place to breed, the seed rearing survival rate is low, can't carry out Industry Promotion.Therefore, the Changjiang river hairtail artificial propagation techniques becomes the key of protection hairtail germ plasm resource and the production of development hairtail large-scale cultivation, and tool is of great significance.
Summary of the invention
The purpose of this invention is to provide the indoor intensive artificial breeding technology of a kind of Coilia ectenes in Yangtze River.
Technical scheme of the present invention comprises reinforced cultivating, artificial induced spawning, artificial incubation and the seed rearing step of cutter long-tailed anchovy parent population, the reinforced cultivating employing that it is characterized in that cutter long-tailed anchovy parent population replaces the cutter long-tailed anchovy adult fish colony of cultivation as parent fish population through 3 years sea water and fresh waters, select again that physically fit vigor is good, scale and the seamless cutter long-tailed anchovy of the complete body surface smooth of fin ray adult fish carry out reinforced cultivating, culturing pool is 20-30m
2Indoor cement pit, breeding density 2-6 tail/m
2, use the fan blower blowing aeration, gas stone density is 0.5-1/m
2The throw something and feed young and the young shrimp of live body seawater oppossum shrimp and Exopalaemon carinicauda, throw something and feed every day 2 times, every day soil pick-up, change weekly water 2 times, fell the pond once every 30-40 days, and culturing pool progressively adds local natural sea-water, improves the culturing pool salinity, rising salt speed is 2-3 ‰/sky, rise at last salt to local natural sea-water salinity, rise the April of Second Year, and culturing pool progressively adds fresh water, salt reduction speed 2-3 ‰/sky, last salt reduction is desalinated in fresh water and is cultivated, and increases simultaneously the air lift facility and makes the pond water flow stimulate parent population in culturing pool, impels its sexual gland further to grow; Parent fish rearing is to by the end of June, water temperature has risen to more than 25 ℃ in the canopy, selection can be seen the soft female fish parent fish individuality of pure and fresh ovary profile, lower abdomen and gently press belly to have the milter parent population individuality that milky seminal fluid flows out, the male and female ratio is 1: 3, parent population moves in the spawning pond after hastening parturition, spawning pond is the rectangle cement pit, area 10m
2, depth of water 1m, hydrostatic, oxygenation, water temperature 25-27 ℃, male and female parent population natural mating spawning and fertilization; Stop to inflate the upper strata that fertilized egg will swim in water after laying eggs, fish for collection with the 80 purpose string bags, Spawning water is fresh water, fertilized egg is adopted temperature control, hydrostatic, inflation hatching, and incubator is that 100 * 100 * 90cm bottom is the cement pit incubator of taper, water body 600kg, put 10000/pond of fertilized egg, inflation hatching in 24 hours, water temperature is controlled at 25-26 ℃, gets final product rupture of membranes through 20 hours hatching fries; Seed rearing is carried out level Four and cultivated step by step: it is that newly hatched larvae is to opening that one-level is cultivated, for newly hatched larvae is supported temporarily, to in incubator, continue hatching behind the fry hatching and carry out postembryonal development, grow and form intestinal tube to mouthpart and get through rear fry and can cultivate by lower storage reservoir Deng fry, incubator changes water once every day between the incubation period, quantity of exchanged water is 60%, aeration quantity should reduce after the hatching, it is injured to avoid inflating the ambassador fry, prelarva grows after 4-5 days substantially opening of fry in incubator, fry is connected water band seedling move in the nursery pond, adopt the hydrostatic inflation method to cultivate; It is that body length is cultivated to 20mm from 3mm that secondary is cultivated, and is the key of cutter long-tailed anchovy summer flower nurture of fish fingerlings, and nursery pond is area 10m
2, depth of water 1m the rectangle cement pit, broadcast 1/m of gas exhausting stone in the pond
2, adopt the hydrostatic blowing aeration to cultivate, the water temperature of growing seedlings is controlled at 25-27 ℃; Breeding density is 3000-5000 tail/m
2The open-mouthed bait of fry is small-sized freshwater rotifer, wheel animalcule density is 3-5/ml, morning and afternoon, each was thrown once, cultivate the feeding volume that replenished afterwards large-scale wheel animalcule in 10 days and increase its palatability, water for larval nursing is fresh water, through injecting nursery pond after with 200 purpose nylon silk cover filterings after pond and the cistern two-stage precipitation, water is not changed in front 5 days seedling ponds of fry rearing not soil pick-up, played the clear end of soil pick-up every day on the 6th day once, changing every other day quantity of exchanged water of water is 50%, and fry is long to total length 20mm, should reach the time-division to support and carry out three grades of cultivations; Three grades of cultivations are that body length is cultivated to 30mm from 20mm, and will cultivate the cutter long-tailed anchovy seedling mesh of growing to 20mm by secondary is that 80 bolting silk net draws in the net to fish for, and with basin or the operation of bucket band water fry are divided fosterly, and nursery pond is area 20m
2, depth of water 1.3m the rectangle cement pit, gas exhausting stone 0.5-1/m
2, adopt the hydrostatic blowing aeration to cultivate, the water temperature of growing seedlings is controlled at 25-27 ℃, and breeding density is 500-1000 tail/m
2The wheel animalcule of throwing something and feeding reaches the young around sufficient class, zooplankton is fished for and throws something and feeds after rear usefulness 40 purpose bolting silks filter, morning and afternoon, each was thrown once, replenish simultaneously the artemia nauplii of throwing something and feeding to increase the nutrition of bait, water for larval nursing is fresh water, through pond and cistern two-stage precipitation with after inject nursery pond behind the 200 purpose nylon silk cover filterings, want the clear end of soil pick-up once every day during the fry rearing, changing every other day quantity of exchanged water of water is 60-70%, it is long to more than the total length 30mm to cultivate fry, should reach the foster level Four of carrying out of time-division and cultivate; It is that body length is cultured to 40-50mm from 30mm that level Four is cultivated, draw in the net to fish for 80 purpose bolting silk nets when the cutter long-tailed anchovy seedling that body reaches the above specification of 30mm is put in a suitable place to breed, with basin or bucket band water operate fry is divided foster, nursery pond area 200m
2, depth of water 1.5m the rectangle cement pit, gas exhausting stone 0.2-0.5/m
2, adopt the hydrostatic blowing aeration to cultivate, the water temperature of growing seedlings is controlled at 25-30 ℃, and breeding density is 50-100 tail/m
2The adult of fresh water around sufficient class and cladocera of throwing something and feeding, zooplankton is fished for 80 purpose bolting silk nets and throws something and feeds after rear usefulness 20 purpose bolting silks filter, and morning and afternoon, each was thrown once, and daily ration, feeding quantity can find still that with the beaker inspection water body with 250ml after throwing something and feeding two hours 1-2 the food organisms such as cyclops are as the criterion; Change weekly water once.Cultivate the fry total length and reach 40-50mm, the time, each fin ray of fry and scale etc. have all been grown complete, enter into the juvenile fish stage, and fry can be put into the pond and be carried out conventional cultivation or sell as the summer flower fingerling.
The present invention with respect to the outstanding feature of prior art is: 1) solved intensive the techniques of mass production of Coilia ectenes in Yangtze River seed, with respect to the rearing of fingerling of present pond extensive style, technology is more ripe, and artificial controllability strengthens; 2) the intensive production of seed rearing, cultivating survival rate further improves, and the unit relative pond rearing of seed output obviously improves, the present invention obtained 2.7 ten thousand in fertilized egg in 2011, newly hatched larvae 2.0 ten thousand tails are cultivated through indoor cement pit, cultivate altogether 40-50mm summer flower fry 1.5 ten thousand tails; Incubation rate 74.1%; Seed rearing survival rate 75.0%; 3) artificial propagation and the middle temperature of growing seedlings, food species, level Four culture technique, a plurality of key nodes of breeding density have been solved, eliminate the relatively fuzzyyer puzzlement of a lot of technical parameters of prior art, improved the precision of each parameter, method more becomes more meticulous, formed operational procedure, easy and simple to handle, be easy to be accepted and promote.
Embodiment
Production technology step of the present invention comprises: reinforced cultivating, artificial induced spawning, artificial incubation and the seed rearing of cutter long-tailed anchovy parent population.
One, the reinforced cultivating of parent population:
1, daily management: use through 3 years sea water and fresh waters alternately the cutter long-tailed anchovy adult fish colony of cultivation select again that physically fit vigor is good, scale and the seamless cutter long-tailed anchovy of the complete body surface smooth of fin ray adult fish carry out reinforced cultivating as parent colony, culturing pool is 20-30m
2Cement pit, breeding density 2-6 tail/m
2, use the fan blower blowing aeration, gas stone density is 0.5-1/m
2, the young of throw something and feed live body seawater oppossum shrimp and Exopalaemon carinicauda and young shrimp, bait adopts bolting silk net (80 order) to fish for, and throws something and feeds every day 2 times, has a small amount of surplus material to be as the criterion when throwing something and feeding with the second time; Every day, soil pick-up changed weekly water 2 times (filtration of 80 orders), fell the pond once every 30-40 days.2, rise salt: the migration of manual simulation's cutter long-tailed anchovy, progressively add local natural sea-water to 11-12 month progressively rising salt every year, and rising salt speed is 2-3 ‰/sky, rises at last salt to local natural sea-water salinity.3, salt reduction: to salt reduction progressively in April of Second Year, progressively add fresh water, salt reduction speed 2-3 ‰/sky, last salt reduction is desalinated in fresh water and is cultivated, and increase air lift facility makes the pond water flow get up to stimulate parent population in cultivating the pond simultaneously, impels its sexual gland further to grow.
Two, artificial induced spawning:
Parent fish rearing is to by the end of June, and water temperature has risen to more than 25 ℃ in the canopy, observes parent population and can see the parent population belly than obviously expanding in the past, and the male and female parent population can be seen the sensation of chasing mutually, and just can carry out artificial induced spawning this moment.
1, the selection of parent population: draw in the net to check and select raun can see pure and fresh ovary profile, the individuality that lower abdomen is soft, milter gently presses belly to have the individuality that milky seminal fluid flows out.The male and female ratio is 1: 3.
2, natural spawning fertilization: parent population moves into after hastening parturition in the spawning pond, and spawning pond is the rectangle cement pit, area 10m
2, depth of water 1m, hydrostatic, oxygenation, water temperature 25-27 ℃, the effect time is 10-12 hour, male and female parent population natural mating spawning and fertilization.
Three, artificial incubation:
1, the collection of fertilized egg: can fish for collection with the 80 purpose string bags.
2, the hatching of fertilized egg: Spawning water is fresh water, fertilized egg is adopted temperature control, hydrostatic, inflation hatching, incubator is that 100 * 100 * 90cm bottom is the cement pit incubator of taper, water body 600kg, put 10000/pond of fertilized egg, inflation hatching in 24 hours, water temperature is controlled at 25-26 ℃, gets final product rupture of membranes through 20 hours hatching fries.
Four, seed rearing:
Cutter long-tailed anchovy seed rearing is carried out level Four and cultivated step by step: one-level is cultivated: newly hatched larvae is to opening; Secondary is cultivated, and body length is cultivated to 20mm from 3mm; Three grades of cultivations, body length is cultivated to 30mm from 20mm; Level Four is cultivated: body length is cultured to 40-50mm from 30mm.
1, one-level is cultivated: will continue hatching behind the fry hatching and carry out postembryonal development in incubator, wait fry to grow to form intestinal tube to mouthpart and get through rear fry and get final product lower storage reservoir and cultivate.Change water once (60%) every day, the minimizing aeration quantity is wavy, and fry opening after 4-5 days connects water band seedling with fry and moves in the nursery pond, adopts the hydrostatic inflation method to cultivate.
2, secondary is cultivated: cutter tapertail anchovy seedling opening is to cultivating to the process of the fingerling of the long 20mm of body: nursery pond is area 10m
2, depth of water 1m the rectangle cement pit, broadcast 1/m of gas exhausting stone in the pond
2, adopt the hydrostatic blowing aeration to cultivate; The water temperature of growing seedlings is controlled at 25-27 ℃; Breeding density is 3000-5000 tail/m
2The open-mouthed bait of fry is small-sized freshwater rotifer, and wheel animalcule density is 3-5/ml, and morning and afternoon, each was thrown once, and cultivating the feeding volume that replenished afterwards large-scale wheel animalcule in 10 days increases its palatability; Water for larval nursing is fresh water, through injecting nursery pond after with 200 purpose nylon silk cover filterings after pond and the cistern two-stage precipitation, water is not changed in front 5 days seedling ponds of fry rearing not soil pick-up, rose in the 6th day and want the clear end of soil pick-up every day once, changing every other day quantity of exchanged water of water is 50%, it is long to about total length 20mm to cultivate fries through about 15 days, should reach the time-division this moment to support and carry out three grades of cultivations.
3, three grades of cultivations: will cultivate the cutter long-tailed anchovy seedling mesh of growing to about 20mm by secondary is that 80 bolting silk net draws in the net to fish for, and fry is divided foster with basin or the operation of bucket band water: nursery pond is area 20m
2, depth of water 1.3m the rectangle cement pit, 0.5/m of gas exhausting stone
2, adopt the hydrostatic blowing aeration to cultivate; The water temperature of growing seedlings is controlled at 25-27 ℃; Breeding density is 500-1000 tail/m
2The ability that the long 20mm fry of body initiatively ingests is stronger, thus bait mainly throw something and feed wheel animalcule and around sufficient class the young, zooplankton is fished for and throws something and feeds after rear usefulness 40 purpose bolting silks filter, morning and afternoon, each was thrown once.Replenish simultaneously some artemia naupliis of throwing something and feeding to increase the nutrition of bait; Water for larval nursing is fresh water, through pond and cistern two-stage precipitation with after inject nursery pond behind the 200 purpose nylon silk cover filterings, want the clear end of soil pick-up once every day during the fry rearing, changing every other day quantity of exchanged water of water is 60-70%.It is long to more than the total length 30mm that process was cultivated fries in about 10-15 days, should reach the foster level Four of carrying out of time-division this moment and cultivate.
4, level Four is cultivated: the cutter long-tailed anchovy seedling that total length reaches the above specification of 30mm will draw in the net minute foster a cultivation again, still draw in the net to fish for 80 purpose bolting silk nets when putting in a suitable place to breed, with basin or bucket band water operate fry is divided foster: nursery pond area 200m
2, depth of water 1.5m the rectangle cement pit, 0.2/m of gas exhausting stone
2, adopt the hydrostatic blowing aeration to cultivate; The water temperature of growing seedlings is controlled at 25-30 ℃; Breeding density is 50-100 tail/m
2The cutter tapertail anchovy seedling ingestion ability that total length reaches 30mm is stronger, so bait mainly throws something and feeds fresh water around the adult of sufficient class and cladocera, and zooplankton is fished for 80 purpose bolting silk nets and throws something and feeds after rear usefulness 20 purpose bolting silks filter, and morning and afternoon, each was thrown once.Daily ration, feeding quantity can find still that with the beaker inspection water body with 250ml after throwing something and feeding two hours 1-2 the food organisms such as cyclops are as the criterion; Soil pick-up does not change weekly water once.Cultivate 10 days left and right sides fry total lengths and reached 40-50mm, this moment, each fin ray of fry and scale etc. were all grown completely, entered into the juvenile fish stage, and fry can be put into the pond and be carried out conventional cultivation or sell as the summer flower fingerling.
Claims (1)
1. indoor intensive artificial breeding technique of Coilia ectenes in Yangtze River, the reinforced cultivating, artificial induced spawning, artificial incubation and the seed rearing step that comprise cutter long-tailed anchovy parent population, the reinforced cultivating employing that it is characterized in that cutter long-tailed anchovy parent population replaces the cutter long-tailed anchovy adult fish colony of cultivation as parent fish population through 3 years sea water and fresh waters, select again that physically fit vigor is good, scale and the seamless cutter long-tailed anchovy of the complete body surface smooth of fin ray adult fish carry out reinforced cultivating, culturing pool is 20-30m
2Indoor cement pit, breeding density 2-6 tail/m
2, use the fan blower blowing aeration, gas stone density is 0.5-1/m
2The throw something and feed young and the young shrimp of live body seawater oppossum shrimp and Exopalaemon carinicauda, throw something and feed every day 2 times, every day soil pick-up, change weekly water 2 times, fell the pond once every 30-40 days, and culturing pool progressively adds local natural sea-water, improves the culturing pool salinity, rising salt speed is 2-3 ‰/sky, rise at last salt to local natural sea-water salinity, rise the April of Second Year, and culturing pool progressively adds fresh water, salt reduction speed 2-3 ‰/sky, last salt reduction is desalinated in fresh water and is cultivated, and increases simultaneously the air lift facility and makes the pond water flow stimulate parent population in culturing pool, impels its sexual gland further to grow; Parent fish rearing is to by the end of June, water temperature has risen to more than 25 ℃ in the canopy, selection can be seen pure and fresh ovary profile, the milter parent population that the soft individual and light pressure belly of female fish parent fish of lower abdomen has milky seminal fluid outflow is individual, the male and female ratio is 1: 3, parent population moves into after hastening parturition in the spawning pond, and spawning pond is the rectangle cement pit, area 10m
2, depth of water 1m, hydrostatic, oxygenation, water temperature 25-27 ℃, male and female parent population natural mating spawning and fertilization; Stop to inflate the upper strata that fertilized egg will swim in water after laying eggs, fish for collection with the 80 purpose string bags, Spawning water is fresh water, fertilized egg is adopted temperature control, hydrostatic, inflation hatching, and incubator is that 100 * 100 * 90cm bottom is the cement pit incubator of taper, water body 600kg, put 10000/pond of fertilized egg, inflation hatching in 24 hours, water temperature is controlled at 25-26 ℃, gets final product rupture of membranes through 20 hours hatching fries; Seed rearing is carried out level Four and cultivated step by step: it is that newly hatched larvae is to opening that one-level is cultivated, for newly hatched larvae is supported temporarily, to in incubator, continue hatching behind the fry hatching and carry out postembryonal development, grow and form intestinal tube to mouthpart and get through rear fry and can cultivate by lower storage reservoir Deng fry, incubator changes water once every day between the incubation period, quantity of exchanged water is 60%, aeration quantity should reduce after the hatching, it is injured to avoid inflating the ambassador fry, prelarva grows after 4-5 days substantially opening of fry in incubator, fry is connected water band seedling move in the nursery pond, adopt the hydrostatic inflation method to cultivate; It is that body length is cultivated to 20mm from 3mm that secondary is cultivated, and is the key of cutter long-tailed anchovy summer flower nurture of fish fingerlings, and nursery pond is area 10m
2, depth of water 1m the rectangle cement pit, broadcast 1/m of gas exhausting stone in the pond
2, adopt the hydrostatic blowing aeration to cultivate, the water temperature of growing seedlings is controlled at 25-27 ℃; Breeding density is 3000-5000 tail/m
2The open-mouthed bait of fry is small-sized freshwater rotifer, wheel animalcule density is 3-5/ml, morning and afternoon, each was thrown once, cultivate the feeding volume that replenished afterwards large-scale wheel animalcule in 10 days and increase its palatability, water for larval nursing is fresh water, through injecting nursery pond after with 200 purpose nylon silk cover filterings after pond and the cistern two-stage precipitation, water is not changed in front 5 days seedling ponds of fry rearing not soil pick-up, played the clear end of soil pick-up every day on the 6th day once, changing every other day quantity of exchanged water of water is 50%, and fry is long to total length 20mm, should reach the time-division to support and carry out three grades of cultivations; Three grades of cultivations are that body length is cultivated to 30mm from 20mm, and will cultivate the cutter long-tailed anchovy seedling mesh of growing to 20mm by secondary is that 80 bolting silk net draws in the net to fish for, and with basin or the operation of bucket band water fry are divided fosterly, and nursery pond is area 20m
2, depth of water 1.3m the rectangle cement pit, gas exhausting stone 0.5-1/m
2, adopt the hydrostatic blowing aeration to cultivate, the water temperature of growing seedlings is controlled at 25-27 ℃, and breeding density is 500-1000 tail/m
2The wheel animalcule of throwing something and feeding reaches the young around sufficient class, zooplankton is fished for and throws something and feeds after rear usefulness 40 purpose bolting silks filter, morning and afternoon, each was thrown once, replenish simultaneously the artemia nauplii of throwing something and feeding to increase the nutrition of bait, water for larval nursing is fresh water, through pond and cistern two-stage precipitation with after inject nursery pond behind the 200 purpose nylon silk cover filterings, want the clear end of soil pick-up once every day during the fry rearing, changing every other day quantity of exchanged water of water is 60-70%, it is long to more than the total length 30mm to cultivate fry, should reach the foster level Four of carrying out of time-division and cultivate; It is that body length is cultured to 40-50mm from 30mm that level Four is cultivated, draw in the net to fish for 80 purpose bolting silk nets when the cutter long-tailed anchovy seedling that body reaches the above specification of 30mm is put in a suitable place to breed, with basin or bucket band water operate fry is divided foster, nursery pond area 200m
2, depth of water 1.5m the rectangle cement pit, gas exhausting stone 0.2-0.5/m
2, adopt the hydrostatic blowing aeration to cultivate, the water temperature of growing seedlings is controlled at 25-30 ℃, and breeding density is 50-100 tail/m
2The adult of fresh water around sufficient class and cladocera of throwing something and feeding, zooplankton is fished for 80 purpose bolting silk nets and throws something and feeds after rear usefulness 20 purpose bolting silks filter, and morning and afternoon, each was thrown once, and daily ration, feeding quantity can find still that with the beaker inspection water body with 250ml after throwing something and feeding two hours 1-2 the food organisms such as cyclops are as the criterion; Change weekly water once.
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