CN102988502A - Preparation method and application of Desheng tablet - Google Patents
Preparation method and application of Desheng tablet Download PDFInfo
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- CN102988502A CN102988502A CN2012103903203A CN201210390320A CN102988502A CN 102988502 A CN102988502 A CN 102988502A CN 2012103903203 A CN2012103903203 A CN 2012103903203A CN 201210390320 A CN201210390320 A CN 201210390320A CN 102988502 A CN102988502 A CN 102988502A
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- 229940079593 drug Drugs 0.000 claims abstract description 15
- 238000000874 microwave-assisted extraction Methods 0.000 claims abstract description 11
- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 10
- 201000009410 rhabdomyosarcoma Diseases 0.000 claims abstract description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 55
- 238000000605 extraction Methods 0.000 claims description 19
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- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 5
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- 229940126678 chinese medicines Drugs 0.000 claims description 5
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- 230000002401 inhibitory effect Effects 0.000 abstract description 3
- 241000382455 Angelica sinensis Species 0.000 abstract 1
- 240000003271 Leonurus japonicus Species 0.000 abstract 1
- 241000112528 Ligusticum striatum Species 0.000 abstract 1
- 235000006484 Paeonia officinalis Nutrition 0.000 abstract 1
- 241001106477 Paeoniaceae Species 0.000 abstract 1
- 230000035755 proliferation Effects 0.000 abstract 1
- 229960004756 ethanol Drugs 0.000 description 21
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- 238000002474 experimental method Methods 0.000 description 8
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- 229940114124 ferulic acid Drugs 0.000 description 8
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 8
- 235000001785 ferulic acid Nutrition 0.000 description 8
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 6
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- 239000013558 reference substance Substances 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
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- 239000012531 culture fluid Substances 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
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- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- 208000004998 Abdominal Pain Diseases 0.000 description 1
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- JWHHANVGNNWIRI-UHFFFAOYSA-N methanol phosphoric acid hydrate Chemical compound O.OC.OP(O)(O)=O JWHHANVGNNWIRI-UHFFFAOYSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention provides a preparation method and applications of a Desheng tablet. The Desheng tablet is prepared from bulk drugs, including 960g of leonurus japonicus, 160g of radix bupleuri, 320g of angelica sinensis, 80g of ligusticum wallichii, 320g of white paeony root and 80g of costustoot, by supercritical extraction and microwave extraction. The invention also provides an application of the Desheng tablet in preparation of drugs for inhibiting proliferation of A-204 cells of rhabdomyosarcoma.
Description
Technical field
The present invention relates to the Chinese medicine preparation technical field, be specifically related to a kind of preparation method and application that gets raw cook.
Background technology
Get raw cook and be recorded in Ministry of Public Health standard WS3-B-0623-91, made as crude drug by Herba Leonuri 960g, Radix Bupleuri 160g, Radix Angelicae Sinensis 320g, Rhizoma Chuanxiong 80g, Radix Paeoniae Alba 320g, Radix Aucklandiae 80g, energy regulating menstruation and nourishing blood, activating QI to eliminate phlegm.Be used for menoxenia, abdominal pain in menstruation, stagnation of QI and blood, mass in the abdomen.
In the prior art, not yet have to such an extent that raw cook adopts the report of supercritical and microwave technology aspect the preparation extracting, and adopt the method that powder and decocting boil of beating, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and used clinically.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of preparation method that gets raw cook.
It is a kind of that raw cook is preparing the application that suppresses in the rhabdomyosarcoma A-204 cell proliferation medicine that another object of the present invention is to provide.
Technical scheme: the objective of the invention is to realize by following scheme:
A kind of preparation method that gets raw cook, made as crude drug by Herba Leonuri 960g, Radix Bupleuri 160g, Radix Angelicae Sinensis 320g, Rhizoma Chuanxiong 80g, Radix Paeoniae Alba 320g, Radix Aucklandiae 80g, described method is comprised of the following step: get Radix Bupleuri, Radix Angelicae Sinensis, Rhizoma Chuanxiong, join in the CO2 supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2Flow 1-3m1/g crude drug min, extraction time 150-180min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W extracts 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
Above-mentioned a kind of preparation method that gets raw cook, described CO
2The percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
Above-mentioned a kind of preparation method that gets raw cook, described microwave extracting power 500W extracts 6 minutes at every turn.
Above-mentioned a kind of preparation method that gets raw cook, described CO
2The extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2Flow 2ml/g crude drug min, extraction time 160min.
Above-mentioned that raw cook is preparing the application that suppresses in the A-204 cell proliferation medicine.
In the prior art, get every 0.5g of raw cook, each 4,2 times on the one, adopt that the present invention is prepared into every 0.5g of raw cook, only need 2 at every turn, took 2 times in 1st, greatly reduced dose having under the condition of more active component.This conclusion can be by following evidence.
The comparison that gets ferulaic acid content in the raw cook of test one, distinct methods preparation
1, instrument and reagent the present invention get raw cook: press the preparation of embodiment 3 methods, use the 1920g crude drug, make 1000 through extraction, every heavy 0.5g.The former raw cook that gets by the preparation of ministry standard method, uses the 1920g crude drug, makes 1700 through extraction, every heavy 0.5g.Agilent 1200 high performance liquid chromatographs; METTLER AE240 electronic analytical balance; Ferulic acid reference substance (Nat'l Pharmaceutical ﹠ Biological Products Control Institute).
2, method
Chromatographic condition and system suitability: be filler with octadecylsilane chemically bonded silica; Methanol-water-phosphoric acid (40:60:0.2) is mobile phase; The detection wavelength is 280nm.Number of theoretical plate is pressed the ferulic acid peak and is calculated, and should be not less than 3000.
The preparation of reference substance solution: precision takes by weighing at 4 hours ferulic acid reference substance of 60 ℃ of drying under reduced pressure an amount of, adds methanol and makes the solution that every 1ml contains 18 μ g, and get final product.
The preparation of need testing solution: get the present invention and get raw cook and the former raw cook that gets, porphyrize, mixing is got 1g, and is accurately weighed, the accurate 70% ethanol 20ml that adds, close plug, supersound process 10 minutes, centrifugal, get supernatant, and get final product.
Algoscopy is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
3, result
The result shows that the content that the present invention gets ferulic acid in the raw cook is the 1.46mg/ sheet; And the former content that gets ferulic acid in the raw cook is the 0.35mg/ sheet, and in the situation that dose reduces, ferulaic acid content improves a lot.
Above-mentioned studies show that, adopt the present invention's preparation raw cook, active constituent content be higher than the ministry standard legal system standby raw cook.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following example, all technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Herba Leonuri 960g, Radix Bupleuri 160g, Radix Angelicae Sinensis 320g, Rhizoma Chuanxiong 80g, Radix Paeoniae Alba 320g, Radix Aucklandiae 80g, with Radix Bupleuri, Radix Angelicae Sinensis, Rhizoma Chuanxiong, join in the CO2 supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4%, extracting pressure 15MPa, 30 ℃ of temperature, CO
2Flow 1m1/g crude drug min, extraction time 150min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 400W extracts 2 times, each 4 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
After testing, the content of ferulic acid is the 1.55mg/ sheet in the finished product.
Embodiment 2
Get Herba Leonuri 960g, Radix Bupleuri 160g, Radix Angelicae Sinensis 320g, Rhizoma Chuanxiong 80g, Radix Paeoniae Alba 320g, Radix Aucklandiae 80g, with Radix Bupleuri, Radix Angelicae Sinensis, Rhizoma Chuanxiong, join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 6%, extracting pressure 30MPa, temperature 50 C, CO
2Flow 3m1/g crude drug min, extraction time 180min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 600W extracts 2 times, each 8 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
After testing, the content of ferulic acid is the 1.54mg/ sheet in the finished product.
Embodiment 3
Get Herba Leonuri 960g, Radix Bupleuri 160g, Radix Angelicae Sinensis 320g, Rhizoma Chuanxiong 80g, Radix Paeoniae Alba 320g, Radix Aucklandiae 80g, with Radix Bupleuri, Radix Angelicae Sinensis, Rhizoma Chuanxiong, join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 20MPa, 40 ℃ of temperature, CO
2Flow 2m1/g crude drug min, extraction time 160min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 500W extracts 2 times, each 6 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
After testing, the content of ferulic acid is the 1.46mg/ sheet in the finished product.
Embodiment 4: get the experimentation data that raw cook suppresses the A-204 cell proliferation
1 experiment material
1.1 experiment cell strain
Rhabdomyosarcoma (A-204), Nanjing Zhengkuan Pharmaceutical Technology Co., Ltd.'s laboratory cell bank, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: the present invention gets raw cook: press the preparation of embodiment 3 methods.
The medicinal liquid liquid storage: take by weighing 100mg and get raw cook, be dissolved in the 5ml dehydrated alcohol, 0.2 μ m filter filters, and 500 μ l doff manage packing ,-20 ℃ of storages, and 0.2 μ m filter filters dehydrated alcohol in order to the usefulness of matched group simultaneously.
1.3 experiment reagent
The Cat.No.12100-061 Lot.No.758137 of DMEM(GIBCO company); Hyclone (Hangzhoupro, sky, Zhejiang bio tech ltd Lot.No.100419); NaHCO3(Shanghai hundred million chemical reagent company limited Cat.No.11810-033Lot.No.1088387 of a specified duration); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt(AMRESCO company lot number: 2010242); Streptomycin Sulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); The autogamy of PBS(laboratory);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); As seen-ultraviolet light microwell plate detector (U.S. MD company model: SPECTRAMAX 190); CO2 incubator (FORMA model: 3111); (safe and sound company of Su Jing group makes model to super-clean bench: SW-CJ-ZFD); Pure water instrument (U.S. Spring company model: S/N 020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (the accurate experimental facilities in Shanghai company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) the A-204 cell carries out cellar culture (10cm culture dish) with DMEM+10%FBS in 37 ℃, 5%CO2, when Growth of Cells during to logarithmic (log) phase, collecting cell discards culture fluid, PBS fine laundering 3 times, add 3ml 0.25% trypsin-0.04%EDTA, behind 37 ℃ of digestion 2min, to wherein adding 5ml complete medium neutralization reaction, behind the piping and druming cell it is changed in the centrifuge tube, the centrifugal 5min of 1000rpm adjusts 3 * 104/ml of concentration of cell suspension.
2) the cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, culture plate put into cell culture incubator (37 ℃, 5%CO2) cellar culture.
3) according to the Growth of Cells situation, generally grow to 50%-70%, add to get raw cook solution, continue to cultivate 24h.
4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) behind the 24h, continue to cultivate 4h.
5) the buckle method is removed supernatant behind the 4h, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on the shaking table, and crystal is fully dissolved.Measure the light absorption value in each hole at enzyme-linked immunosorbent assay instrument 490nm place.
6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide) is set 6 multiple holes for every group.
7) result represents with the suppression ratio of medicine to cell:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value * 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel 2003 softwares, data represent with mean ± S.D..
4 experimental results
Statistical result showed after the mtt assay experiment, compare with matched group, when dosage reaches 5mg/ml, to A-204 cell inhibitory effect variant (P＜0.05), dosage this difference when 10mg/ml has significance (P＜0.01), and utmost point significant difference (P＜0.001) is arranged when dosage reaches 15-20mg/ml.
Table 1 gets raw cook to A-204 cell inhibitory effect impact research
Annotate: compare with matched group,
*P＜0.01;
*P＜0.001
5 experiment conclusion
Get raw cook and can suppress the A-204 cell proliferation, reduce the Growth of Cells number of A-204 cell, this effect is dose dependent.
Claims (5)
- One kind the preparation method of raw cook, made as crude drug by Herba Leonuri 960g, Radix Bupleuri 160g, Radix Angelicae Sinensis 320g, Rhizoma Chuanxiong 80g, Radix Paeoniae Alba 320g, Radix Aucklandiae 80g, it is characterized in that described method is comprised of the following step: get Radix Bupleuri, Radix Angelicae Sinensis, Rhizoma Chuanxiong, join CO 2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO 2Flow 1-3m1/g crude drug min, extraction time 150-180min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W extracts 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
- 2. the preparation method of described a kind of raw cook according to claim 1 is characterized in that described CO 2The percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
- 3. the preparation method of described a kind of raw cook according to claim 1 is characterized in that described microwave extracting power 500W, extracts 6 minutes at every turn.
- 4. the preparation method of described a kind of raw cook according to claim 1 is characterized in that described CO 2The extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO 2Flow 2ml/g crude drug min, extraction time 160min.
- 5. described a kind of that raw cook is preparing the application that suppresses in the rhabdomyosarcoma A-204 cell proliferation medicine according to claim 1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210390320.3A CN102988502B (en) | 2012-10-15 | 2012-10-15 | Preparation method and application of Desheng tablet |
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| CN201210390320.3A CN102988502B (en) | 2012-10-15 | 2012-10-15 | Preparation method and application of Desheng tablet |
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| CN102988502A true CN102988502A (en) | 2013-03-27 |
| CN102988502B CN102988502B (en) | 2014-01-08 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103610794A (en) * | 2013-11-26 | 2014-03-05 | 常州科立信医疗器械有限公司 | Preparation method and application of nerve-calming tablets |
| CN103784574A (en) * | 2014-01-28 | 2014-05-14 | 济南伟传信息技术有限公司 | Orally taken traditional Chinese medicine for preventing and treating hemolytic disease of newborn |
| CN105582132A (en) * | 2016-03-07 | 2016-05-18 | 南京多宝生物科技有限公司 | Preparation method and application of liquorice root formulation granules |
| CN105748542A (en) * | 2016-03-07 | 2016-07-13 | 南京多宝生物科技有限公司 | Preparation method and application of angelica sinensis formula granules |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1368213A (en) * | 2001-02-01 | 2002-09-11 | 杨孟君 | Nano medicine 'Desheng' and its preparing process |
| CN102397451A (en) * | 2011-11-26 | 2012-04-04 | 苏州派腾生物医药科技有限公司 | Preparation method of cholagogic tablet |
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2012
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103610794A (en) * | 2013-11-26 | 2014-03-05 | 常州科立信医疗器械有限公司 | Preparation method and application of nerve-calming tablets |
| CN103610794B (en) * | 2013-11-26 | 2015-07-15 | 青岛大学附属医院 | Preparation method and application of nerve-calming tablets |
| CN103784574A (en) * | 2014-01-28 | 2014-05-14 | 济南伟传信息技术有限公司 | Orally taken traditional Chinese medicine for preventing and treating hemolytic disease of newborn |
| CN103784574B (en) * | 2014-01-28 | 2016-04-06 | 济南伟传信息技术有限公司 | A kind of Chinese medicine for oral administration preventing and treating hemolytic disease of newborn |
| CN105582132A (en) * | 2016-03-07 | 2016-05-18 | 南京多宝生物科技有限公司 | Preparation method and application of liquorice root formulation granules |
| CN105748542A (en) * | 2016-03-07 | 2016-07-13 | 南京多宝生物科技有限公司 | Preparation method and application of angelica sinensis formula granules |
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|---|---|
| CN102988502B (en) | 2014-01-08 |
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