CN102939924B - Method for artificially breeding sepia lycidas gray - Google Patents
Method for artificially breeding sepia lycidas gray Download PDFInfo
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- CN102939924B CN102939924B CN201210452053.8A CN201210452053A CN102939924B CN 102939924 B CN102939924 B CN 102939924B CN 201210452053 A CN201210452053 A CN 201210452053A CN 102939924 B CN102939924 B CN 102939924B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a method for artificially breeding sepia lycidas gray, and the method is characterized by comprising the following steps of (1) parent breeding: selecting a harmless parent with complete body into a temporary culture cement tank to be temporarily cultivated, feeding the parent with chilled fishes twice a day, wherein the feeding quantity is 2 to 5 percent of the weight of the sepia lycidas gray; (2) artificial breeding: placing an egg attaching device inside the temporary culture cement tank, mating the male and the female parents to lay eggs, and then transferring the eggs into a hatching tank to be hatched; and (3) offspring breeding: transferring the hatched seedlings into a seedling tank, feeding fairy shrimp nauplius or copepoda in the early stage, feeding copepoda, opossum shrimp or larval prawn in the middle stage, and feeding fresh live fishes and small shrimps in the late stage, completing the breeding of the seedling when the length of the seedling is more than 2cm, the trunk length is more than 1.2cm, the trunk width is more than 0.9cm and the weight is more than 0.3g, and taking out the seedlings with water. The method has the advantages of high survival rate, easiness in cultivation, large cultivation specification, strong disease resistance and fast growth.
Description
Technical field
The present invention relates to a kind of aquatic product animal raise seedling method, especially relate to a kind of method of intending order cuttlefish artificial breeding.
Background technology
Plan order cuttlefish (
sepia lycidas) be subordinate to Cephalopoda (Cephalopoda), Sepiida (Sepioidea), Sepiidae (Sepiidae
), Sepia (
sepia), be the kind of dwelling the end that shallow sea water warm is stronger, perch in the offshore waters of continental shelf district 15～100m depth of water, be distributed in the Indian Ocean-Western Pacific.At South China Sea, spawning season is February in October to next year, and the depth of water 60～100m in this period place resource is compared with horn of plenty; Carry out migration to offshore 15～30m depth of water place 3～May.Ovum invests on the materials such as gorgonian, sargassum, bamboo branch or cord more.Intend large (the long 40cm of maximum trunk of order cuttlefish build, body weight 5kg), breeding cycle is short, growth rapidly, delicious meat, protein content is high, and comprehensive utilization scope is wide, therefore be a kind of breed variety that has cultivation prospect, the higher important fishery kind in improved seeds, tw Taiwan of Ta Yeshi China South Sea economic worth simultaneously.
Since 2003, University Of Ningbo has done more research at the aspect such as reproductive biology, aquaculture technology of Sepiella maindroni.But aspect propagating artificially, find Sepiella maindroni sex premature, bad phenomenon such as individual little (50g/ are only), fanning economics is not high, has seriously hindered the development of cuttlefish aquaculture.Therefore, select individuality larger, order cuttlefish is intended in growth faster, is the effective way that solves this bottleneck.Few to the research of plan order cuttlefish both at home and abroad at present, mainly concentrate on reproductive behavior, epidermis collagen, the young to CO
2tolerance, fertilization behavior, predation etc., there is not yet up to now the correlative study report of intending order cuttlefish artificial breeding.
Summary of the invention
It is high that technical problem to be solved by this invention is to provide a kind of survival rate, is easy to cultivation, and forming specification is large, and premunition is strong, the method for the plan order cuttlefish artificial breeding of growing fast.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of method of intending order cuttlefish artificial breeding, comprises the following steps:
(1) parents culture: select the bodily form complete, send into temporary curing cement pond without the plan order cuttlefish parent of injury and support temporarily, and the chilled small fish of 10～15cm size of throwing something and feeding, day bait throwing in 2 times, feeding volume is to intend 2%～5% of order cuttlefish weight; Wherein the temporary foster requirement of cement pit is as follows,
A. condition of culture: parent adopts airing form to cultivate, periodic replacement is through the seawater of precipitation, sand filtration, and a day quantity of exchanged water is 100%, removes every day such as residual bait etc., keeps cultivating 14～25 DEG C of water temperatures, salinity 28～30, dissolved oxygen is 10～14.50mg/ L, pH 7.81～8.04;
B. breeding density: male and female are raised together with, female-male proportion is 2:1～3:1, breeding density is with 1～3/m
2be advisable;
(2) artificial propagation
A. lay eggs: in described temporary curing cement pond, place egg apparatus, male and female parent carries out after mating, internal fertilization, female plan order cuttlefish lays eggs to egg apparatus, and the water temperature of laying eggs is 16.4～25.0 DEG C, and salinity is 28～30;
B. hatching: the fertilized egg obtaining after laying eggs is transferred in hatchery, in the plastics screen frame of hatchery and bolting silk screen frame, hatch, incubation temperature is 21～27 DEG C, salinity is 25～30, brooding time 21d～35d, Spawning water is successively through sand filtration, obscure shallow lake and acrylic staple fibre bag filtration, and individual layer sunshade net is added a cover in top, hatchery;
(3) seed rearing: the seedling of incubation of membrane is moved on in seedling pond, throw something and feed early stage through 3～5mm fairy shrimp nauplius of single-cell algae strengthening or the copepoda of 1.5～5mm, food density is 1～2/mL; Throw something and feed the mid-term copepoda of 1.5～13mm, oppossum shrimp or the 0.5～1.0cm larvae of Penaeus vannamei of 5～25mm, food density is 5～10/L; Throw something and feed fresh and alive small fish and 2.0～5.0cm small shrimps of later stage, when seedling total length is greater than 2cm, trunk is grown up in 1.2cm, and trunk is wider than 0.9cm, and when body weight is greater than 0.3g, seedling culture completes, and band water is emerged, and wherein seedling pool environment requires as follows:
A. nurturing an environment: it is 21～27 DEG C that water temperature is cultivated in described seedling pond, and salinity is that 23～30, pH is 7.81～8.04, and the depth of water 1.0～1.5 is advisable, every 1～2m
2place a gas exhausting stone, every day, quantity of exchanged water was 50～60%, and removed in time dead individuality and residue dirt;
B. young density: in described seedling pond, seedling is with 1000/m
3～1500/m
3be advisable.
The middle parent of step (1) is sent into the transport lorry adopting in the process in temporary curing cement pond 2.0 × 1.5. × 1.0m is housed
3nylon canvas, the ocean temperature filling in described transport lorry is 14～25 DEG C, salinity is 28～30, and is filled with oxygen.
Temporary curing cement pond size described in step (1) is 5.0 × 4.3 × 1.0m
3, in described temporary curing cement pond, be evenly provided with 8～12 inflating air stones.
Splash in described temporary curing cement pond in step (1) povidone iodine of 10～20mg/L.Because cuttlefish in handling process has slight damage, anticorrosion for it is carried out disinfection.
Spawning pond top described in step (2) is evenly provided with some for hanging the stationary rope of described egg apparatus, described stationary rope spacing is each other 70-90cm, described egg apparatus upper end is fixed on described stationary rope, there is the sinker of heavy 0.1-0.2kg in its lower end system, and at the bottom of the pond of described egg apparatus and described spawning pond, leaves the space of 4-5cm.
The described egg apparatus of step (2) is that mesh is the polythene net sheet of 25-30mm, it is 0.8-1m that described polythene net sheet is evenly suspended on spacing distance on described stationary rope and on every stationary rope, and described polythene net sheet is of a size of 30cm × 40cm.Because the surface area of net sheet is larger, in embryo development procedure, between fertilized egg, be not easy to cause extruding, and simple for production, can throw on a large scale.
The described egg apparatus of step (2) is the polythene net sheet of mesh 25-30mm and the nylon rope that diameter is 8mm-12mm, unified described polythene net sheet or the described nylon rope of hanging on stationary rope described in every, the stationary rope that hangs described polythene net sheet comes with the stationary rope space that hangs described nylon rope, the spacing distance of described polythene net sheet on described stationary rope is 0.8-1m, the spacing distance of described nylon rope on described stationary rope is 0.8-1m, described polythene net sheet is of a size of 30cm × 40cm, the length 1.5m of described nylon rope.Due to the surface area of net sheet and the diameter of nylon rope larger, in embryo development procedure, between fertilized egg, be not easy to cause extruding, and simple for production, can throw on a large scale.
In step (2), intending order hatching cuttlefish temperature is 24 DEG C, and salinity is 30, and hatching density is controlled at 3～6/L.Under this incubation temperature, salinity and density, can ensure higher incubation rate, shorter incubation cycle, cultivation period, and ensure the absorption ratio that lower first abnormal rate, yolk sac of incubating the young is higher, the larger first young.
The process that in step (2), fertilized egg is transported to from temporary curing cement pond to hatchery is: fertilized egg adopts the transportation resources of double-layer nylon bag oxygenation, and oxygenation bag specification is 75 × 75 × 55cm
3, put into subsequently 48 × 48 × 25cm
3bubble chamber in, bubble chamber dress ovum 4～5kg, is filled with water 1/3, when transport, water temperature is 22 DEG C, salinity is 30.
Step (3) bait with throw something and feed, early stage is for intending the stage that the trunk length of order cuttlefish is 0.8～1.0cm, be to intend the stage that the trunk length of order cuttlefish is 1.0～2.0cm in mid-term, the later stage is the stage of intending the long > 2.0cm of trunk of order cuttlefish.
Compared with prior art, the invention has the advantages that: this seedling-cultivating method has the advantages such as incubation rate is high, survival rate is high, disease is few, the amount of growing seedlings is high, be easy to cultivation, grow soon, trimmed size is large, and the operation easy to implement of this seedling-cultivating method, success rate are high, suitable large-scale promotion.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
One, specific embodiment
Embodiment 1
A kind of method of intending order cuttlefish artificial breeding of the present invention, specifically comprises the following steps:
1, parents culture
(1) parent source and transport
Mid-March～late April is with the colony of Zhaoan County, Zhangzhou City, small-sized trawl fishing Fujian Province natural waters egg-laying migration.Select the bodily form complete, put into without the parent of injury the device that fills seawater and oxygenation and support temporarily, and deliver to as early as possible temporary curing cement pond.When transport, adopt 2.0 × 1.5. × 1.0m is housed
3the buggy of nylon canvas, it is 14～25 that temperature is housed on car, the seawater that salinity is 28～30, and oxygenation.Transport used time 0.5h, average transportation survival rate is 95.26%, once find that there is ink-jet individuality, should immediately prepared Chinese ink be dragged for or water is changed in full pond;
(2) parent reinforced cultivating
A. breeding condition
Curing cement pond is 5.0 × 4.3 × 1.0m temporarily
3cement pit, 8～12 inflating air stones are evenly set in pond, breeding water is nature seawater through precipitation, sand filtration, water quality is limpid, cultivates 14～25 DEG C of water temperatures, salinity 28～30, dissolved oxygen is 14.50mg/ L, pH 7.81～8.04.Because cuttlefish in handling process has slight damage, the povidone iodine of the 10～20mg/L that need splash in temporary curing cement pond.Once find that there is ink-jet individuality, should immediately prepared Chinese ink be dragged for or be changed water;
B. breeding density
Male and female are raised together with, and female ratio is that (2:1)～(3:1), breeding density is with 1/m
2～3/m
2be advisable;
C. bait with throw something and feed
The chilled small fish of 10～15cm size of throwing something and feeding, day bait throwing in 2 times, feeding volume is 2%～5% of cuttlefish weight;
D. water quality management
Parent adopts airing form to cultivate, periodic replacement seawater, and a day quantity of exchanged water is 100%.Remove every day such as residual bait etc.Keep 14～25 DEG C of water temperatures, salinity 28～30, dissolved oxygen is > 10mg/ L, pH 7.81～8.04.
2, artificial propagation
?(1) making of egg apparatus and placement
Above spawning pond, be evenly provided with some stationary ropes, each stationary rope spacing is each other 70-90cm, get mesh and be the polythene net sheet of 25-30mm as egg apparatus, evenly being suspended on spacing distance on stationary rope and on every stationary rope is 0.8-1m, this polythene net sheet is of a size of 30cm × 40cm, egg apparatus upper end is fixed on stationary rope by connecting rope, the sinker of heavy 0.1-0.2kg is fastened in its lower end, make it in stretching state, and make to leave at the bottom of the pond of egg apparatus and spawning pond the space of 4-5cm.Parent is placed egg apparatus before laying eggs.Egg apparatus need be through potassium permanganate strict sterilization, and fresh seawater is rushed after quiet and laid.Clean the not egg apparatus of attached ovum 1 time every 2～3d;
(2) male and female are differentiated
General male is large compared with female individuals, and male body colour is yellowish-brown, more bright-coloured, and female is taupe; The male back side " eye mouthful " decorative pattern is larger, left side the 4th wrist stem, and the sucker at full wrist middle part suddenly diminishes and is sparse.Male in order to fight for spouse, can open as possible 4 pairs of wrists (except tactile wrist) and fight, occur that warning colouration, body colour become extremely bright-coloured simultaneously.Ripe male dissection is visible larger ink sac, spermary, spermatophore sac and sperm-storing bag afterwards.Adult female is dissected by-product ovum gland, the ovary of rear visible a pair of yellow;
(3) lay eggs
Intending order cuttlefish is internal fertilization, and when mating, male and female are tightly embraced together by each wrist, mating complete separately after a few minutes, femalely under the accompanying of hero, to egg apparatus, lay eggs.The water temperature of laying eggs is 16.4～25.0 DEG C, and salinity is 28～30.To common property ovum 237.5kg on April 23, be about 59375 from March 16,261.5 of average every inkfish spawnings;
(4) transport of fertilized egg
Fertilized egg adopts the transportation resources of double-layer nylon bag oxygenation, and oxygenation bag specification is 75 × 75 × 55cm
3, put into subsequently 48 × 48 × 25cm
3bubble chamber in.A bubble chamber can fill at most ovum 4～5kg, is filled with water 1/3.When transport, water temperature is 22 DEG C, and salinity is 30.For fear of serious extruding, affect hatchability of fertile eggs, need to keep flat neatly and transport being again attached with the rope of ovum and net sheet.When packaging and transport, be all careful and put down gently, to reduce the stimulation to fertilized egg, raise for fear of water temperature in transportation simultaneously, need in each bubble chamber, put into 2 ice chests;
(5) hatching
A. environment of hatching
Fertilized egg is placed in plastics screen frame and bolting silk screen frame hatches; Hatchery is 4.0 × 8.0 × 1.6m
3, plastics screen frame is 55 × 55 × 20cm
3, bolting silk screen frame is 50 × 50 × 100cm
3; Hatching salinity is 25～30, and temperature is 21～27 DEG C.Spawning water is through sand filtration, obscure shallow lake and acrylic staple fibre bag filtration, and individual layer sunshade net is added a cover in top, hatchery;
B. brooding time
In normal water temperature range, cuttlefish embryonic development required time and water temperature height are inversely proportional to, under the condition of 27～30 DEG C of water temperatures, and average brooding time 17～24d; At 21～27 DEG C of water temperatures, average brooding time 24d～35d;
C. hatch results
Hatch altogether 23050 of cuttlefishes from April 16 to May 20.Just incubate long (8.1 ± 0.2) mm of cuttlefish trunk, trunk wide (6.1 ± 0.2) mm, body weight (0.18 ± 0.02) g.
3, seed rearing
(1) nurturing an environment: the seedling of incubation of membrane is moved on to seedling pond, and seedling pond is 4.0 × 8.0 × 1.6m
3, salinity is 23～30, and temperature is 21～27 DEG C, and the depth of water 1.0～1.5m is advisable, every 1～2m
2place a gas exhausting stone;
(2) Larval quality: after young cuttlefish membrane, at the bottom of being active in pond, can swim in looking for food at the middle and upper levels night, energetic be the healthy and strong young; Otherwise be the young inferior;
(3) daily management
A. bait with throw something and feed
Intending order cuttlefish is carnivorous siphonopods, the young cuttlefish zooplanktons such as copepoda, cladocera or oppossum shrimp of liking ingesting, the adult live bait such as fish, shrimp of liking ingesting.Specifically as shown in table 1.
The each phase food species of table 1 and feeding volume
The seedling of incubation of membrane is moved on in seedling pond, throw something and feed early stage through 3～5mm fairy shrimp nauplius of single-cell algae strengthening or copepoda, food density is 1～2/mL; Throw something and feed the mid-term copepoda of 1.5～13mm, oppossum shrimp or the 0.5～1.0cm larvae of Penaeus vannamei of 5～25mm, food density is 5～10/L; Throw something and feed fresh and alive small fish and 2.0～5.0cm small shrimps of later stage, when seedling total length is greater than 2cm, trunk is grown up in 1.2cm, and trunk is wider than 0.9cm, and when body weight is greater than 0.3g, seedling culture completes, and band water is emerged;
B. water quality management
Salinity is 23～30, and temperature is 21～27 DEG C, and pH is 7.81～8.04.Under normal circumstances, every day, quantity of exchanged water was 50～60%, and removed in time dead individuality and residue dirt.The siphonage of sampling while changing water, grasps the principle that slow-motion goes out slowly, in order to avoid water environment changes the excessive cuttlefish ink-jet that causes.Between the megathermal period, will change a little fresh seawater much more as far as possible, illumination will cover sunshade net when too strong.At heavy rain or typhoon weather, notice that salinity changes, salinity sharply reduces and easily causes cuttlefish discomfort even dead, need to slowly add crude salt to regulate, to keep salinity luffing to be no more than 5 ‰;
C. inflation
Along with cuttlefish growth, its oxygen demand also increases, therefore can adopt micro-inflation the early stage of growing seedlings, the later stage should be strengthened aeration quantity;
(4) emerge, pack and transport
A. specification and quality
Selecting specification is total length 2cm, the long 1.2cm of trunk, the wide 0.9cm of trunk, body weight 0.3g, and size evenly, body surface totally, not damaged and deformity, plan order cuttlefish that vigor is stronger;
B. emerge
While emerging, first Chi Shui is drained into after the about 30cm of the depth of water, to drum, be then with water to count one by one and put into Nylon Bag with soft diddle-net band water collection seedling;
C. packaging
The general transportation resources that adopts Nylon Bag oxygenation.Specification 35 × 35 × 40cm
3nylon Bag can put 100 seeds, specification 75 × 75 × 55cm
3nylon Bag can put 200 seeds, put into subsequently 48 × 48 × 25cm
3bubble chamber in.Seed transport should select shady and cool weather and morning and evening to carry out, and water temperature is 24 DEG C while transporting, and salinity is 25;
D. transportation survival rate
Packaging time is all careful and is put down gently with transport, to reduce the stimulation to seed, otherwise can cause seed ink-jet and affects transportation survival rate.Transport on May 8 cuttlefish seed used time 12h, survival rate 86%; Transport on May 23 seed used time 3h, survival rate 95%.
Embodiment 2
With embodiment 1, its distinguishing characteristics is: 14 DEG C of (1) parents culture water temperatures, and salinity 28, dissolved oxygen is 10mg/ L, pH 7.81; (2) water temperature of laying eggs is 16.4 DEG C, and salinity is 28; (3) hatching salinity is 25, and temperature is 21 DEG C, average brooding time 34d; (4) seed rearing temperature is 21 DEG C, and salinity is that 23, pH is 7.81; (5) when seedling total length is 3cm, trunk is grown up in 1.5cm, and trunk is wide is 1.2cm, and when body weight is 0.5g, seedling culture completes.(6) stationary rope spacing is each other 80cm, egg apparatus upper end is fixed on stationary rope, there is the sinker of heavy 0.1-0.2kg in its lower end system, and at the bottom of the pond of egg apparatus and spawning pond, leave the space of 4-5cm, above-mentioned egg apparatus is the polythene net sheet of mesh 25-30mm and the nylon rope that diameter is 8mm-12mm, unified polythene net sheet or the nylon rope of hanging on every stationary rope, the stationary rope that hangs polythene net sheet comes with the stationary rope space that hangs nylon rope, the spacing distance of polythene net sheet on stationary rope is 1m, the spacing distance of nylon rope on stationary rope is 1m, polythene net sheet is of a size of 30cm × 40cm, the length 1.5m of nylon rope, (7) seedling of incubation of membrane is moved on in seedling pond, throw something and feed through 3～5mm fairy shrimp nauplius of single-cell algae strengthening early stage, and food density is 1/mL, the throw something and feed mid-term copepoda of 1.5～13mm, food density is 5/L, throw something and feed fresh and alive small fish and 2.0～5.0cm small shrimps of later stage.
Embodiment 3
With embodiment 1, its distinguishing characteristics is: 25 DEG C of (1) parents culture water temperatures, and salinity 30, dissolved oxygen is 12.50mg/ L, pH 8. 04; (2) water temperature of laying eggs is 25.0 DEG C, and salinity is 30; (3) hatching salinity is 30, and temperature is 27 DEG C, average brooding time 24d; (4) seed rearing temperature is 27 DEG C, and salinity is that 30, pH is 8.04; (5) as seedling total length 2.5cm, the long 1.4cm of trunk, the wide 1.1cm of trunk, when body weight 0.4g, seedling culture completes; (6) seedling of incubation of membrane is moved on in seedling pond, the copepoda of the 1.5～5mm that throws something and feeds early stage, food density is 2/mL; The throw something and feed mid-term oppossum shrimp of 5～25mm, food density is 8/L; Throw something and feed fresh and alive small fish and 2.0～5.0cm small shrimps of later stage.
Embodiment 4
With embodiment 1, its distinguishing characteristics is: 20 DEG C of (1) parents culture water temperatures, and salinity 29, dissolved oxygen is 13.50mg/ L, pH 8; (2) water temperature of laying eggs is 22 DEG C, and salinity is 29; (3) hatching salinity is 28, and temperature is 25 DEG C, average brooding time 28d; (4) seed rearing temperature is 23 DEG C, and salinity is that 26, pH is 7.9; (5) when seedling total length is greater than 2cm, the long 1.5cm of trunk, the wide 1.3cm of trunk, when body weight 0.35g, seedling culture completes; (6) seedling of incubation of membrane is moved on in seedling pond, the copepoda of the 1.5～5mm that throws something and feeds early stage, food density is 2/mL; 0.5～1.0cm larvae of Penaeus vannamei of throwing something and feeding mid-term, food density is 5～10/L; Throw something and feed fresh and alive small fish and 2.0～5.0cm small shrimps of later stage.
Certainly, above-mentioned explanation is not limitation of the present invention, and the present invention is also not limited to above-mentioned giving an example.Those skilled in the art are in essential scope of the present invention, and variation, remodeling, interpolation or the replacement made, also should belong to protection scope of the present invention, and protection scope of the present invention is as the criterion with claims.
Claims (10)
1. intend a method for order cuttlefish artificial breeding, it is characterized in that comprising the following steps:
(1) parents culture: select the bodily form complete, send into temporary curing cement pond without the plan order cuttlefish parent of injury and support temporarily, and the chilled small fish of 10～15cm size of throwing something and feeding, day bait throwing in 2 times, feeding volume is to intend 2%～5% of order cuttlefish weight; Wherein the temporary foster requirement of cement pit is as follows,
A. condition of culture: parent adopts airing form to cultivate, periodic replacement is through the seawater of precipitation, sand filtration, and a day quantity of exchanged water is 100%, removes every day such as residual bait etc., keeps cultivating 14～25 DEG C of water temperatures, salinity 28～30, dissolved oxygen is 10～14.50mg/L, pH7.81～8.04;
B. breeding density: male and female are raised together with, female-male proportion is 2:1～3:1, breeding density is with 1～3/m
2be advisable;
(2) artificial propagation
A. lay eggs: in described temporary curing cement pond, place egg apparatus, male and female parent carries out after mating, internal fertilization, female plan order cuttlefish lays eggs to egg apparatus, and the water temperature of laying eggs is 16.4～25.0 DEG C, and salinity is 28～30;
B. hatching: the fertilized egg obtaining after laying eggs is transferred in hatchery, in the plastics screen frame of hatchery and bolting silk screen frame, hatch, incubation temperature is 21～27 DEG C, salinity is 25～30, brooding time 21d～35d, Spawning water is successively through sand filtration, obscure shallow lake and acrylic staple fibre bag filtration, and individual layer sunshade net is added a cover in top, hatchery;
(3) seed rearing: the seedling of incubation of membrane is moved on in seedling pond, throw something and feed early stage through 3～5mm fairy shrimp nauplius of single-cell algae strengthening or the copepoda of 1.5～5mm, food density is 1～2/mL; Throw something and feed the mid-term copepoda of 1.5～13mm, oppossum shrimp or the 0.5～1.0cm larvae of Penaeus vannamei of 5～25mm, food density is 5～10/L; Throw something and feed fresh and alive small fish and 2.0～5.0cm small shrimps of later stage, when seedling total length is greater than 2cm, trunk is grown up in 1.2cm, and trunk is wider than 0.9cm, and when body weight is greater than 0.3g, seedling culture completes, and band water is emerged, and wherein seedling pool environment requires as follows:
A. nurturing an environment: it is 21～27 DEG C that water temperature is cultivated in described seedling pond, and salinity is that 23～30, pH is 7.81～8.04, and the depth of water 1.0～1.5m is advisable, every 1～2m
2place a gas exhausting stone, every day, quantity of exchanged water was 50～60%, and removed in time dead individuality and residue dirt;
B. young density: in described seedling pond, seedling is with 1000/m
3～1500/m
3be advisable.
2. a kind of method of intending order cuttlefish artificial breeding according to claim 1, is characterized in that: the middle parent of step (1) is sent into the transport lorry adopting in the process in temporary curing cement pond 2.0 × 1.5. × 1.0m is housed
3nylon canvas, the ocean temperature filling in described transport lorry is 14～25 DEG C, salinity is 28～30, and is filled with oxygen.
3. a kind of method of intending order cuttlefish artificial breeding according to claim 1, is characterized in that: the temporary curing cement pond size described in step (1) is 5.0 × 4.3 × 1.0m
3, in described temporary curing cement pond, be evenly provided with 8～12 inflating air stones.
4. according to a kind of method of intending order cuttlefish artificial breeding described in claim 1 or 3, it is characterized in that: the povidone iodine of the 10～20mg/L that splashes in described temporary curing cement pond in step (1).
5. a kind of method of intending order cuttlefish artificial breeding according to claim 1, it is characterized in that: the spawning pond top described in step (2) is evenly provided with some for hanging the stationary rope of described egg apparatus, described stationary rope spacing is each other 70-90cm, described egg apparatus upper end is fixed on described stationary rope, there is the sinker of heavy 0.1-0.2kg in its lower end system, and at the bottom of the pond of described egg apparatus and described spawning pond, leaves the space of 4-5cm.
6. a kind of method of intending order cuttlefish artificial breeding according to claim 5, it is characterized in that: the described egg apparatus of step (2) is that mesh is the polythene net sheet of 25-30mm, it is 0.8-1m that described polythene net sheet is evenly suspended on spacing distance on described stationary rope and on every stationary rope, and described polythene net sheet is of a size of 30cm × 40cm.
7. a kind of method of intending order cuttlefish artificial breeding according to claim 5, it is characterized in that: the described egg apparatus of step (2) is the polythene net sheet of mesh 25-30mm and the nylon rope that diameter is 8mm-12mm, unified described polythene net sheet or the described nylon rope of hanging on stationary rope described in every, the stationary rope that hangs described polythene net sheet comes with the stationary rope space that hangs described nylon rope, the spacing distance of described polythene net sheet on described stationary rope is 0.8-1m, the spacing distance of described nylon rope on described stationary rope is 0.8-1m, described polythene net sheet is of a size of 30cm × 40cm, the length 1.5m of described nylon rope.
8. a kind of method of intending order cuttlefish artificial breeding according to claim 1, is characterized in that: in step (2), intending order hatching cuttlefish temperature is 24 DEG C, and salinity is 30, and hatching density is controlled at 3～6/L.
9. a kind of method of intending order cuttlefish artificial breeding according to claim 1, it is characterized in that the process that in step (2), fertilized egg is transported to from temporary curing cement pond to hatchery is: fertilized egg adopts the transportation resources of double-layer nylon bag oxygenation, and oxygenation bag specification is 75 × 75 × 55cm
3, put into subsequently 48 × 48 × 25cm
3bubble chamber in, bubble chamber dress ovum 4～5kg, is filled with water 1/3, when transport, water temperature is 22 DEG C, salinity is 30.
10. a kind of method of intending order cuttlefish artificial breeding according to claim 1, it is characterized in that: step (3) bait with throw something and feed, early stage is for intending the stage that the trunk length of order cuttlefish is 0.8～1.0cm, be the stage that the trunk length of plan order cuttlefish is 1.0～2.0cm mid-term, and the later stage is the stage of intending the long > 2.0cm of trunk of order cuttlefish.
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| CN103548733B (en) * | 2013-10-30 | 2016-06-22 | 浙江海洋学院 | Tiger spot sepiellae seu sepiae method for culturing seedlings |
| CN103548732B (en) * | 2013-10-30 | 2016-01-13 | 浙江海洋学院 | Tiger spot cuttlefish artificial seed breeding method |
| CN103598125A (en) * | 2013-11-19 | 2014-02-26 | 浙江海洋学院 | Running water type artificial seed cultivation method for sepia lycidas gray |
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| CN109362610B (en) * | 2018-03-22 | 2021-06-25 | 中国水产科学研究院黄海水产研究所 | Method for cultivating fry of sepia reiliana |
| CN109430118B (en) * | 2018-08-27 | 2021-05-11 | 吴常文 | Method for improving reproductive capacity of cuttlefish |
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