CN109362610B - Method for cultivating fry of sepia reiliana - Google Patents

Method for cultivating fry of sepia reiliana Download PDF

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CN109362610B
CN109362610B CN201810241984.0A CN201810241984A CN109362610B CN 109362610 B CN109362610 B CN 109362610B CN 201810241984 A CN201810241984 A CN 201810241984A CN 109362610 B CN109362610 B CN 109362610B
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CN109362610A (en
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刘长琳
陈四清
翟介明
孙礼娟
肖志忠
葛建龙
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Biodiversity & Conservation Biology (AREA)
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Abstract

The invention relates to a method for cultivating Sepia rebaudiana fry, and belongs to the technical field of aquaculture. A method for cultivating the fry of the cuttlefish in the Leishi manner is characterized by comprising the following steps: A. domesticating and culturing parents; B. collecting fertilized eggs; C. hatching fertilized eggs; D. cultivating seedlings; the hatching method solves the problems that a large number of oothecas are stuck together, the permeability is poor, the hatching temperature is high, the hatching time is long, and the ootheca is easily rotten due to bacterial infection.

Description

Method for cultivating fry of sepia reiliana
Technical Field
The invention relates to a method for cultivating Sepia rebaudiana fry, and belongs to the technical field of aquaculture.
Background
The Sepiella maindroni (Sepoteutis leisoniana Lesson) belongs to mollusks, class cephalopoda, class coleoidea, order of gun, order of anomala, family of Sepiidae, genus Sepiella, is a large-scale Sepiella maindroni, and is mainly distributed in south of Fujian and coastal areas of Guangdong, and also distributed in Japan, Hawaii, Malaysia, India offshore areas and Red sea. The Sepiella maindroni is a secondary economic cephalopod in the hot water region of the Western Pacific, is usually harvested in the coastal waters of the Guangdong and Min in China, has the annual yield of about 200t, has large body (the largest body weighs up to 5.6 kilograms), has thick meat, higher edible parts and tender and delicious meat quality, is a seafood good product after being dried, has extremely high edible, nutritional, medicinal and economic values, and is deeply favored by consumers in China, Korea, Japan and the like. In recent years, due to the fact that a great amount of roe parents are caught, the resource amount is sharply reduced and almost exhausted, and therefore, the research on breeding and breeding of the Sepiella leinii is imperative.
Compared with the cephalopods developed in China at present, the Sepiella maindroni has the characteristics of large individual (the maximum weight can reach 5.6 kilograms), high growth speed (the product specification can be reached in 6-7 months under the condition of proper temperature and bait), large fecundity (about 4000 grains), capability of eating icy fresh bait under the culture condition and the like, and has wide development prospect. However, no report on the technology for breeding the sepia lesonii fries is found so far.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a novel method for cultivating the sepia leishmania fries.
The invention is realized by adopting the following technical scheme:
a method for cultivating the fry of the cuttlefish in the Leishi manner comprises the following steps: A. domesticating and culturing parents; B. collecting fertilized eggs; C. hatching fertilized eggs; D. cultivating seedlings;
the step B comprises the following small steps:
a) placing a fertilized egg attaching base in a culture pond, wherein the attaching base has a three-dimensional branch structure; the three-dimensional branched structure is a structure similar to a branch shape, and any structure in which branches are branched from the main stem can be used as the fertilized egg attachment base of the present invention. Preferably, the diameter of the trunk of the three-dimensional branched structure is not more than 5cm, the diameter of the branch is preferably 0.5-1cm, and the occupation area of each attaching base is 1-3m2,20m2Approximately 5 and 6 of the attachment bases are placed in the culture pond.
b) According to the egg laying condition, the attachment base for hanging eggs is removed periodically to prevent the egg sheaths from being too concentrated and supplement new attachment bases with equal quantity;
the step C comprises the following small steps:
c) cutting off the part with the egg masses on the attaching base, disinfecting and washing with clear water;
d) tying the washed attaching bases with the egg masses to the thin ropes, tying two ends of the thin ropes to two sides of the net cage, hanging the attaching bases with the egg masses, and putting the attaching bases into an incubation pool for incubation;
e) when the eggs are hatched to 6-9d and the egg type is fixed and the egg section is obvious, cutting off a group of 5-8 egg sheaths along the combination part of the egg sheaths and the attachment base, and putting the cut egg sheaths into an incubation basket for continuous incubation;
f) hatching about 20 days to obtain larvae;
in the early stage of hatching, namely hatching for 0-15d, and pouring boxes or baskets once at intervals of 3-4 d; in the late hatching period, namely after 16d of hatching, basket separation is carried out;
step C satisfies the conditions: the water temperature is 22-26 ℃;
during the incubation period, water is changed for 2-3 times every day, wherein the water change amount is 200% -300%.
Oosperms of the squids are 6-9cm long, 8 oosperms are wrapped in each oosperms on average, the squids are favored to lay the oosperms at the same position, the oosperms are concentrated, the shape of an oosphere formed by the oosperms is special, the weight of the oosperms is large, and the attachment of the oosperms cannot be achieved by using the existing squids attachment base. Through comparative research on various shapes, the fact that the three-dimensional branch structure, namely the dendritic attachment base is used as the attachment base of the fertilized eggs of the cuttlefish leisurely, the egg hanging rate is high, the attachment is tight and is not easy to fall off, and the attachment effect is best.
After the three-dimensional branch structure attaching bases are uniformly stacked and placed, the middle of each attaching base is provided with a hole, the water permeability is good, and the attaching bases are put together to form a stable structure with the holes, so that egg masses can be conveniently hung. After the hanging of the egg masses is completed, the positions where the egg masses are hung can be cut off, and one end of the cut attaching bases is hung on the rope to lift the egg masses for hatching.
And the box or basket is turned over in the early stage of hatching to eliminate water flow dead zones inside the egg masses, increase the water fluidity, avoid extrusion and prevent rotting.
The hatching method solves the problems that a large number of oothecas are stuck together, the permeability is poor, the hatching temperature is high, the hatching time is long, and the ootheca is easily rotten due to bacterial infection.
The sterilization in the small step c) meets the conditions: the egg masses are sterilized in 400-500ppm iodine solution for 40-60 s.
After the fertilized eggs of the cuttlefish swell, the permeability of the egg membranes is increased, and the embryo bodies are stimulated by disinfectant to easily cause ink jet in the eggs to die, so that the fertilized eggs of the cuttlefish are carefully disinfected in the prior art and are not usually disinfected; but if the bacteria are not disinfected, the bacteria are easy to breed to influence the survival rate. Therefore this application adopts the quick sterile mode of high concentration, and under the short time, the antiseptic solution has not permeated the egg membrane yet, neither can stimulate the inkfish embryoid inkjet, also can avoid the adverse effect that bacterial growing caused simultaneously.
The attachment base in the small step a) is a branch, preferably a poplar branch.
In the research, the poplar branches are used as the attachment base, and the egg hanging rate is the highest. The probable reason is that the branches of the poplars with the poplar leaves are more similar to aquatic plants after being put into water, and are more favored by the squid.
The step D comprises the following small steps:
g) transferring the larva into a seedling culture pond for culture;
h) pouring the larvae into the pond when the larvae are 30-40 days old, and performing pond separation in combination;
d, feeding the larvae into mysidacea, Chinese hooked shrimp and exopalaemon carinicauda;
the bait feeding mode is as follows: feeding mysidacea in 2-30 days, feeding Chinese hooked shrimp in 20-50 days, and feeding palaemon carinicauda in 40 days.
The Sepiella maindroni belongs to the ocean variety and can not adapt to the feeding mode of other Sepiella maindroni. Therefore, according to the method, the bait for the Rohdea rivieri is redesigned according to the types of shrimps encountered in the migration process of the Rohdea rivieri, the original hook shrimps are added to serve as the transition bait, and the survival rate of the Rohdea rivieri can be obviously improved.
Feeding large individual varieties when baits are fed in 20-30 days old and 40-50 days old, and feeding small individual varieties after 20-30 min; feeding for 1-2 times per day; changing water for 2-4 times per day, and cleaning bottom for 1 time per day.
If the shrimp seeds at the two stages of age of 20-30 days and age of 40-50 days are not distinguished, the shrimp seeds are directly fed, and the feed intake of the larvae is less due to the size of the apertures; if the feeding method is adopted, the food intake of the Sepiella maindroni can be obviously improved by firstly feeding large individual shrimp seeds and then feeding small individual shrimp seeds.
The feeding amount of the bait is such that the live bait remains in the pond.
The squid leishmania has strong activity and generally swims in the middle and upper layers of water, so that the bait is preferably fed to the middle and upper layers of water. The feeding amount is preferably that a small amount of live baits are left in the pond.
The step D satisfies the condition: the cultivation density is 500-600/m3(ii) a Adopting running water cultivation, and gradually increasing the water change amount from 100-200% to more than 400% along with the growth of the larva; the water temperature is 19-26 ℃, the daily temperature difference is less than 1 ℃, the pH is 7.8-8.2, the salinity is 28-32, the dissolved oxygen is more than 6mg/L, and the gas is continuously inflated.
In the step d), the specification of the net cage ensures that the water flow exchange is good and the ootheca cannot leak out; in the step e), the specification of the basket is as follows: the diameter is 30-40cm, the basket height is 10-15cm, and the basket eye diameter is 2-5 mm.
The step A satisfies the condition: the caught wild parents meet the conditions: the parent has normal color and luster, strong activity, positive ingestion and the weight of more than 700 g; domestication and cultivation meet the conditions: density of 3-5 pieces/m3Water body, male-female ratio of 1: 1-1: 1.5, water temperature of 20-26 ℃, salinity of 28-32, pH of 7.8-8.2, dissolved oxygen of more than 5mg/L and illumination intensity of 400lx-800 lx; the culture is carried out by running water, and the water exchange amount is 400-700%. The bait is live fish and live shrimp, the daily feeding amount is 2% -3% of the weight of the cuttlefish, the feeding is carried out for 2 times/day, excrement, residual bait and dead individuals are cleaned every day, and water is thoroughly changed. When water is changed, the dead bait and the squid larva are fished out.
And D, E, daily management after seedling cultivation: pouring or dividing the pond for 1 time per month according to the culture density; and (5) taking the offspring seeds out of the pool when the trunk length of the offspring seeds reaches 5-6 cm.
Pouring or dividing the pond for 1 time per month according to the culture density, sampling, measuring the body length and the body weight, and calculating the bait coefficient; when the individual difference is significant, one specification screening is carried out. The bait coefficient is the bait feeding amount/the individual weight growth amount, and the body length weight and the bait coefficient are counted, so that the breeding effect can be comprehensively analyzed, and a basis is provided for subsequent parameter optimization. The specification screening is the prior art in the field, and the invention is not improved.
And (5) taking the offspring seeds out of the pool when the trunk length of the offspring seeds reaches 5-6 cm.
Culturing at water temperature of 19-26 deg.C for 60-70 days until the trunk length of the offspring seed reaches 5-6cm, and taking out.
Detailed Description
In order to make the object and technical solution of the present invention more apparent, the present invention is described in further detail below.
The first embodiment is as follows:
A. domesticating and culturing parents:
the parents are individuals cultivated in an industrial overwintering way or wild parents caught in coastal sea areas of Fujian and Guangdong from the beginning of 5 months to 8 months every year. Normal color of parent, strong activity, positive ingestion, weight of more than 700g, and breeding density of 3-5 pieces/m3Water body, male-female ratio of 1: 1-1: 1.5, water temperature of 20-26 ℃, salinity of 28-32, pH of 7.8-8.2, dissolved oxygen of more than 5mg/L and illumination intensity of 400lx-800 lx. The culture is carried out by running water, and the water exchange amount is 400-700%. The bait is live fish and live shrimp, the daily feeding amount is 2% -3% of the weight of the cuttlefish, the bait is fed for 2 times every day, excrement, residual bait and dead individuals are cleaned every morning, and water is thoroughly changed.
B. Collecting fertilized eggs:
and a certain amount of poplar branches are required to be placed in the cultivation pond as an attachment base for attachment of the fertilized eggs. The poplar branches are uniformly placed according to a certain angle, and the volume of each attachment base is about 1-3m3,20m2Approximately 5 and 6 of the attachment bases are placed in the culture pond. Avoiding the air stone and the water outlet so as to avoid influencing the spawning of the parent. The egg-bearing anchorage was removed daily while an equal amount of new anchorage was replenished. The squids in Lei's love to lay the ootheca in the same position, the ootheca is concentrated, and the ootheca laid each day is generally concentrated on 1-2 attachment groups to form 2-4 egg masses. The size of the egg mass is related to the number of parents, the period of time in which the eggs are laid (mid, full or late), and the duration of the eggs laid. The average daily egg laying amount of 20 female cuttlefish per pond is about 8-10 kg.
C. Hatching fertilized eggs:
cutting off the part with the egg masses on the attachment base, putting the cut part into 400ppm iodine solution for disinfection for 40s, washing the cut part with the clean water, putting the cut part into an incubation pool for incubation, tying branches with the egg masses onto a thin rope in order to prevent mutual extrusion, tying two ends of the thin rope on two sides of a net cage, and incubating the egg masses 10-20cm away from the bottom of the net cage, wherein the aperture of the net cage is 0.3-5 mm, which is suitable for good water flow exchange and no leakage of egg sheaths. When the eggs are hatched to 6-9 days and the egg shapes are fixed and the egg sections are obvious, cutting off a group of 5-8 egg sheaths along the joint part of the egg sheaths and the attachment base, putting the cut eggs into a plastic basket for hatching, wherein the diameter of the plastic basket is 30-40cm, the height of the basket is 10-15cm, and the diameter of a basket eye is 2-5 mm. The fertilized eggs are poured out of the box or basket once at intervals of 3-4 days in the early hatching period (0-15d), and the egg membranes are gradually expanded in the late hatching period (16d and later), mainly by basket division. The seedlings are hatched successively at the water temperature of 22-26 ℃ for about 20 days. During the incubation period, water is changed for 2-3 times every day, wherein the water change amount is 200% -300%.
D. And (3) seedling cultivation:
after the larvae are hatched out, all the larvae are scooped out on the water surface one by using water, the larvae are transferred into a fry breeding pool for breeding after counting, the breeding density is 500-3. The larva is cultivated by running water, the water exchange amount is gradually increased along with the growth of the young cuttlefish, according to the water quality condition, the water is changed by 100-200% every day in the initial stage, and the water is gradually increased to more than 400% in the later stage. The water for cultivation is used after precipitation and sand filtration, the water temperature is 19-26 ℃, the daily temperature difference is less than 1 ℃, the pH is 7.8-8.2, the salinity is 28-32, the dissolved oxygen is more than 6mg/L, and the air is continuously inflated. And (4) after about 30-40 days old, using a small landing net to drive the seedlings into a plastic basin, carrying out water-carrying operation to prevent ink jet, pouring the seedlings into the basin, and carrying out pond separation.
The series of larval breeding baits are mysid, Chinese hooked shrimp and palaemon carinicauda. Feeding Mysidacea on young cuttlefish at 2-30 days old, feeding Pandalus sinensis at 20-50 days old, and feeding palaemon carinicauda at 40 days old. In the overlapping period of bait feeding, large individual shrimp seeds are fed firstly, and small individual shrimp seeds are fed after 25 min. The bait is flushed clean when being fed, and is fed for 2 times every day, and the feeding amount is preferably that a small amount of live bait is left in the pond. Changing water 3 times every day, cleaning the bottom once, fishing out the dead bait and cuttlefish larva by using a small dip net when changing water, and thoroughly changing water.
E. Daily management:
the workshop is required to be frequently patrolled, the abnormal conditions of gas, water quality and temperature are checked, and the records are made. And (4) pouring or dividing the pond for 1 time according to the culture density every month, sampling, measuring the body length and the body weight, calculating the bait coefficient, and comprehensively analyzing the culture effect. When the individual difference is significant, one specification screening is carried out.
Under the condition of water temperature of 19-26 ℃, the trunk length of the seedlings reaches 5-6cm through cultivation for 60-70 days, and the seedlings can be sold as commercial seedlings.
The hatching rate of the fertilized eggs reaches 90 percent by statistics; the survival rate of the offspring seed cultivation reaches 75 percent.
Example two:
the difference from the first example is that the attachment base is made of branches of plane tree, and the average daily egg laying amount is about 5-7 kg.
Example three:
the difference from the first example is that the attachment base is a dendritic attachment base made of plastic, and the daily average egg laying amount is about 3-5kg under the same conditions.
Example four:
the difference from the first embodiment is that the sterilization method is to sterilize in 30ppm iodine solution for 5min, and the hatching rate of the fertilized eggs is 70 percent; the survival rate of the fry cultivation is 50 percent.
Example five:
the difference from the first example is that the larval culture baits are mysid and palaemon carinicauda. Feeding mysorethorn for 2-40 days old young cuttlefish, and feeding palaemon carinicauda after 40 days old, wherein the survival rate of fry breeding is 30%.
The invention breaks through the large-scale breeding technology of the sepia reiliana fries for the first time.

Claims (9)

1. A method for cultivating the fry of the cuttlefish in the Leishi manner is characterized by comprising the following steps: A. domesticating and culturing parents; B. collecting fertilized eggs; C. hatching fertilized eggs; D. cultivating seedlings;
the step B comprises the following small steps:
a) placing a fertilized egg attachment base in a culture pond, wherein the attachment base is a branch;
b) removing the attachment base for egg hanging, and supplementing new attachment bases with equal quantity;
the step C comprises the following small steps:
c) cutting off the part with the egg masses on the attaching base, disinfecting and washing with clear water;
d) tying the washed attaching base with the egg masses to the thin ropes, tying two ends of the thin ropes to two sides of the net cage, and incubating;
e) when the eggs are hatched to 6-9d and the egg type is fixed and the egg section is obvious, cutting off a group of 5-8 egg sheaths along the combination part of the egg sheaths and the attachment base, and putting the egg sheaths into a basket for continuous hatching;
f) hatching about 20 days to obtain larvae;
in the early stage of hatching, namely hatching for 0-15d, and pouring boxes or baskets once at intervals of 3-4 d; in the late hatching period, namely after 16d of hatching, basket separation is carried out;
step C satisfies the conditions:
the water temperature is 22-26 ℃; the disinfection meets the conditions: the egg masses are sterilized in 400-500ppm iodine solution for 40-60 s.
2. The method for cultivating sepia reishi offspring seeds according to claim 1, wherein the attaching base in the small step a) is poplar branch.
3. The cuttlefish fry rearing method according to claim 1, wherein the step D comprises the following substeps:
g) transferring the larva into a seedling culture pond for culture;
h) pouring the larvae into the pond when the larvae are 30-40 days old, and performing pond separation in combination;
using mysorethorn, Chinese hooked shrimp and palaemon carinicauda as larva baits in the step D; the bait feeding mode is as follows: feeding mysidacea in 2-30 days, feeding Chinese hooked shrimp in 20-50 days, and feeding palaemon carinicauda in 40 days.
4. The method for cultivating the sepia reicheri fries according to claim 3, wherein the large individual species are fed first when the baits are fed at the ages of 20-30 days and 40-50 days, and the small individual species are fed after 20-30 min; feeding for 1-2 times per day; changing water for 2-4 times per day, and cleaning bottom for 1 time per day.
5. The cuttlefish fry rearing method according to claim 3, wherein the amount of bait fed is such that live bait remains in the pond.
6.The cuttlefish fry rearing method according to claim 1, wherein the step D satisfies the condition: the cultivation density is 500-600 pieces/m3(ii) a Adopting running water cultivation, and gradually increasing the water change amount from 100-200% to more than 400% along with the growth of the larva; the water temperature is 19-26 ℃, the daily temperature difference is less than 1 ℃, the pH value is 7.8-8.2, the salinity is 28-32, and the dissolved oxygen is more than 6 mg/L.
7. The method for cultivating the sepiella rillii seeds of claim 1, wherein in the step d), the specification of the net cage is such that the water flow exchange is good and the ootheca can not leak out; in the step e), the specification of the basket is as follows: the diameter is 30-40cm, the basket height is 10-15cm, and the basket eye diameter is 2-5 mm.
8. The cuttlefish fry rearing method according to claim 1, wherein the step a satisfies the condition: the caught wild parents meet the conditions: the parent has normal color and luster, strong activity, positive ingestion and the weight of more than 700 g; domestication and cultivation meet the conditions: density of 3-5 pieces/m3Water body, male-female ratio of 1: 1-1: 1.5, water temperature of 20-26 ℃, salinity of 28-32, pH of 7.8-8.2, dissolved oxygen of more than 5mg/L and illumination intensity of 400lx-800 lx; adopting running water cultivation, wherein the water exchange amount is 400-700%; the bait is live fish and live shrimp, the daily feeding amount is 2% -3% of the weight of the cuttlefish, the feeding is carried out for 2 times/day, excrement, residual bait and dead individuals are cleaned every day, and water is thoroughly changed.
9. The cuttlefish fry rearing method according to claim 1, further comprising D, E after fry rearing, daily management steps of: pouring or dividing the pond for 1 time per month according to the culture density; and (5) taking the offspring seeds out of the pool when the trunk length of the offspring seeds reaches 5-6 cm.
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CN109757406B (en) * 2019-02-27 2021-11-05 日照市海洋与渔业研究所(日照市海域使用动态监视监测中心、日照市水生动物救护站) Sepia esculenta fry proliferation method
CN109864019B (en) * 2019-04-19 2022-04-15 中国水产科学研究院黄海水产研究所 Hatching system for fertilized eggs of sepia reiliana and using method
CN113749021B (en) * 2021-10-12 2022-05-13 中国水产科学研究院黄海水产研究所 Method for cultivating sepia duchenne fries

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