CN102919579B - Eggs rich in omega-3 polyunsaturated fatty acids and preparation method - Google Patents
Eggs rich in omega-3 polyunsaturated fatty acids and preparation method Download PDFInfo
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Abstract
The invention relates to a preparation method for eggs rich in omega-3 polyunsaturated fatty acids. The preparation method comprises the following steps of: (1) adding screened rubber seed kernel or rubber seed oil cake into non-gm feed for laying hens, with the additive amount of the rubber seed kernel being 10-20% of the total weight of the feed for laying hens, and the additive amount of the rubber seed oil cake being 15-25% of the total weight of the feed for laying hens; (2) adjusting ingredients of the feed to enable the feed to comprise the following ingredients: 42.89 of corn, 17-32 of soybean meal, 0.59 of wheat bran, 2-3 of lard, 10-20 of rubber seed kernel or 15-25 of rubber seed oil cake, 0.35 of salt, 8.38 of stone powder, 1.8 of calcium hydrogen phosphate, 0.1 of lysine, 0.14 of methionine, 1 of premix for laying hens, and 5 international units of vitamin E per kg of feed; (3) feeding 130-140-day laying hens by using the feed for laying eggs rich in omega-3 polyunsaturated fatty acids after feeding for 2-4 weeks. The preparation method is low in cost, stable in content of the omega-3 polyunsaturated fatty acids, and safe without peculiar smell, and effectively and sufficiently utilizes rubber seed resources.
Description
Technical field
The present invention relates to the serial polyunsaturated fatty acid egg in a kind of ω of being rich in-3 and preparation method.
Background technology
The serial polyunsaturated fatty acids in ω-3 (ω-3PUFAs), belong to long-chain unsaturated fatty acid (having 18-22 carbon atom), mainly comprise
(LNA), eicosapentaenoic acid (EPA, containing 5 unsaturated double-bonds) and DHA (DHA, containing 6 unsaturated double-bonds).Omega-fatty acid in the process of body metabolism is: health is converted into eicosapentaenoic acid (EPA) by the alpha-linolenic acid (LNA) of taking in, and health is converted into EPA again DHA (DHA).By the formation of Fatty acids oxidation process, having the active material regulating of varied organisms is absorbed by the body.
Alpha-linolenic acid, EPA, DHA are bringing into play respectively different effects in vivo.
in body metabolism activity, fight for same enzyme material with the serial polyunsaturated fatty acid in ω-6 simultaneously, after alpha-linolenic acid supplements, can limit in time the conversion of the serial polyunsaturated fatty acid in ω-6, it also has hemangiectasis simultaneously, reduce blood viscosity, reduce the effect of triglyceride; EPA penetrates into blood platelet immobilized artificial membrane in vivo, become the chief component of mitochondria and cell membrane, and metabolism generation prostanoid, thromboxane and leukotriene, this class material directly has influence on keep-alive and the function of human body, thereby health is produced to vital effect; DHA accounts for cerebral cortex lipid 10%, and directly decider's intellectual level, will cause decrease of memory once lack, and dementia even occurs, and DHA also determines children's retinal development simultaneously.
Because alpha-linolenic acid is very slow in the metabolism of people's syntaxy, scientist advises that valetudinarian should supplement alpha-linolenic acid, EPA, tri-kinds of materials of DHA simultaneously.Be rich in having of the serial polyunsaturated fatty acid in ω-3: fish oil, linseed oil, walnut oil, rubber seed oil, green vegetable is as purple perilla, marine alga etc.
Not long ago, FAO (Food and Agriculture Organization of the United Nation), the World Health Organization combined and issued a statement: need worldwide promote the serial polyunsaturated fatty acid in ω-3.For this life and health important substance of the serial polyunsaturated fatty acid in ω-3, countries in the world scientist has published achievement in research more than 15000 at authoritative Scientific Periodicals up to now, becomes already the most popular scientific research project in biotechnology field.
Although these two groups of polyunsaturated fatty acids of ω-3 and ω-6 are essential fatty acids, but human body can not self synthesize, can only from food, absorb, when if the ratio of these two groups of essential fatty acids of ω-3 and ω-6 reaches the level in a healthy and balanced way of 1:4, it is the most efficient that the function of human body will reach, it is the strongest that health is resisted various diseases ability, the most powerful to body-building and life-prolonging.At present, in China's meals, the ratio of unrighted acid ω-6 and ω-3 is up to 20:1～30:1, this is because the soybean oil that China resident mainly takes in, peanut oil cause containing the serial polyunsaturated fatty acid in ω-6, and that abundant deep sea fish oil, linseed oil, the walnut wet goods of the serial content of polyunsaturated fatty acid in ω-3 eaten is considerably less.It is to be noted especially the high meals of the serial unrighted acid in ω-6, although reduced deaths from heart disease rate, but increased cancer and insulin resistance diabetes the incidence of disease [yellow luxuriant and bdautiful etc. birds, beasts and eggs ω-3 series, the serial content of polyunsaturated fatty acid research in ω-6. grain and grease, 2009(12): 21-22].Increase the serial polyunsaturated fatty acid intake in ω-3 and can promote brain, nervous system and amphiblestroid growth.The serial polyunsaturated fatty acid in ω-3 reduces the generation of human body angiocardiopathy and inflammation by all means, reduces Diabetes Mellitus LDL-C (LDL-C) and triglycerides (TG) level.The content that increases the serial polyunsaturated fatty acid in ω-3 in meals can have adjuvant treatment effect to patients such as angiocardiopathy, diabetes, inflammation; The serial polyunsaturated fatty acid in ω-3 can act on nerve cell, to noting depression, schizophrenia and absent mindedly having certain curative effect.The serial polyunsaturated fatty acid in ω-3 also can suppress the hyperplasia of mammary gland, prostate and the colon cancer cell of in vitro culture, promotion Apoptosis [Chen Yinji etc. the serial polyunsaturated fatty acid prevention of disease in ω-3 and therapeutic action. Chinese oil, 2006(9): 31-34].In addition, old encephalatrophy is chronic encephalopathy common in old man.According to the U.S. recent studies on, show, if vitamin C in old human body, D, E, the serial levels of polyunsaturated fatty acids of B family vitamin and ω-3 are higher, occur that the risk of this disease may reduce.China people are solving the today of having enough to eat and wear, the popular obviously deficiency of the serial polyunsaturated fatty acid in ω-3 of taking in, and the content that how to improve the serial polyunsaturated fatty acid in ω-3 in diet structure has been subject to extensive concern.
The method that increases at present the serial polyunsaturated fatty acid in ω-3 in egg is not a lot, Lan Taifu etc. disclose a kind of egg feed addictive prescription and production technology [application number: 200710051023.5] that is rich in omega-fatty acid, the method is in chicken feed, to add dandelion, date, spirulina, bitter dish and longevity greens/mustard green, and these additives may affect the mouthfeel of egg.In layer diets, add a certain proportion of linseed, fish oil or fish meal in addition, but linseed exists dispute to laying hen and the impact of laying eggs, fish oil or fish meal also have the report that reduces egg yolk weight, also can reduce the mouthfeel of egg simultaneously, also have peculiar smell.It is generally acknowledged that rational fish oil interpolation level is 1%～1. 5%, fish meal is according to geographical environment, acceptable excursion is 2%～10%, and the interpolation level of linseed will be lower than 10%, otherwise affect egg quality [R.Gonzalez-Esquerra et al.:Can.J.Anim.Sci., 2001,295-305].In addition, linseed add the content that membership changes DHA and EPA in yolk, but the total content to both does not have a significant effect, and the prolongation along with the linseed time of feeding, DHA and EPA total amount present reduction trend [Shen Yong etc. the impact of linseed on DHA in egg yolk and EPA content. Journal of Nutrition, 2012(34): 50-54].
Along with people are to healthy growing interest, in the daily meals of the mankind, the serial polyunsaturated fatty acid intake in ω-3 is not enough, therefore develops the food, the health products that are rich in the serial polyunsaturated fatty acid in ω-3 and becomes very necessary.
Egg is owing to being rich in high-quality protein, phosphatide and other nutrients, becomes one of the most edible nutriment of the mankind.Yet the content of the serial polyunsaturated fatty acid in ω-3 is lower in common egg, common egg only has 30～70mg/100g, so exploitation has the egg of the serial polyunsaturated fatty acid in ω-3 of high level, has wide market prospects.
The market report of Canada flax association shows, the market share meeting sustainable growth of ω-3 egg.At present, omega-3 egg accounts for whole 15% of the shell egg market that connects.The consumption survey of 1994 Nian U.S. Texas shows, the consumer of acceptance investigation over half is ready to spend more money to buy polytrophic ω-3 egg.Along with the raising of concept of health, the market share of ω-3 egg can continue to increase.Singapore, Malaysian market have ω-3 egg to sell; Through preliminary investigation, in each large supermarket in Hong Kong and convenience store, similar egg is less; The great market on interior ground is also blank substantially.
Summary of the invention
The object of the invention is to for the serial polyunsaturated fatty acid ratio of the serial polyunsaturated fatty acid in current ω-3 and ω-6 unbalanced, and the problem of ω-3 series fatty acid intake wretched insufficiency, the serial polyunsaturated fatty acid egg in a kind of ω of being rich in-3 and preparation method are provided.The method have be of high nutritive value, safety, product without fishy smell, aboundresources, cost is low, concentration effect is remarkable, can make full use of the features such as rubber seed resource again.
Rubber tree is topmost economic tree in Euphorbiaceae, in South Subtropical Area of China, widely plants.Every annual meeting produces a large amount of glue seeds, rubber seed is rich in LNA and the rear remaining rubber seed oil cake of oil expression and also contains the more nutriments such as LNA, can be used as bird feed additive, increase the content of the serial polyunsaturated fatty acid in ω-3 in poultry and birds, beasts and eggs, and this resource is not yet being utilized aspect this.
The present invention adds rubber seed or rubber seed oil cake in the daily raising of laying hen, makes to contain the serial polyunsaturated fatty acid in ω-3 in egg, can solve on the one hand the problem of China ω-3 serial polyunsaturated fatty acid intake deficiency; Improve on the other hand the serial content of polyunsaturated fatty acid in ω-3 in egg, realized the enrichment of the serial polyunsaturated fatty acids in ω-3; The third aspect is added in chicken feed and is made egg be rich in the serial polyunsaturated fatty acid in ω-3 with rubber seed or rubber seed oil cake, and the rubber tree resource of China's abundant is fully utilized.
To achieve these goals, technical scheme of the present invention is:
(1) the rubber rubber seed of screening or rubber seed oil cake are added in non-transgenic egg feedstuff, rubber seed adds by 10～20% of egg feedstuff total amount, and rubber seed oil cake adds by 15～25% of egg feedstuff total amount;
(2) adjust feed composition, composition is consisted of: corn 42.89, dregs of beans 17～32, wheat bran 0.59, lard 2～3, rubber seed 10～20 or rubber seed oil cake, 15～25, salt 0.35, stone flour 8.83, calcium monohydrogen phosphate 1.8, lysine 0.1, methionine 0.14, laying hen premix 1, in per kilogram feed, the content of vitamin E is 5 international units;
(3) with above-mentioned feed, feed the laying hen of 130～140 ages in days, feed the egg that 2～4 weeks can output be rich in the serial polyunsaturated fatty acid in ω-3.
Compared with prior art, advantage is as follows in the present invention:
1, resource is extensive.In the present invention, the rubber seed using or rubber seed oil cake, derived from seeds of rubber tree and rubber seed oil expression after oil cake, and the rubber distinctive plant that is Yunnan Province, cultivated area is large, rubber seed resource is sufficient.
2, concentration effect is good.In rubber seed, contain the serial polyunsaturated fatty acid in higher ω-3, in its rubber seed oil cake, be also rich in the serial polyunsaturated fatty acid in ω-3, chicken feed was used after the feed containing rubber seed or rubber seed oil cake, and the serial polyunsaturated fatty acid in ω-3 can transform in vivo, is enriched in yolk.
3, safety.Rubber seed pure natural used in the present invention, there is not potential safety hazard in the oil cake after oil expression, so the egg that is rich in the serial polyunsaturated fatty acid in ω-3 of producing does not exist the food-safety problem that may cause yet.
4, without fishy smell.Rubber seed or rubber seed oil cake, without fishy smell and other smells, join in chicken feed, and the egg of production does not have fishy smell and other peculiar smell.
5, cost is low.The rubber seed oil cake using in the present invention is the residue of rubber seed after squeezing is processed, and is added in chicken feed, belongs to the recycling of resource, has reduced and has produced the cost that is rich in ω-3 serial polyunsaturated fatty acid egg product.
6, of far-reaching significance.The egg that is rich in the serial polyunsaturated fatty acid in ω-3 in the present invention, can be used as efficiently, without fishy smell, safe nutrition product, is applied in food and health products, provides ω-3 serial polyunsaturated fatty acid.The present invention not only solves the problem of China ω-3 serial polyunsaturated fatty acid intake wretched insufficiency, improve the serial content of polyunsaturated fatty acid in ω-3 in egg, also reduce the content of cholesterol in egg and the rubber tree waste resource of China's abundant is fully used.And another approach for the mankind provide the serial polyunsaturated fatty acids in absorption ω-3, has profound significance to making full use of with health of resource.
The specific embodiment
Embodiment 1
Add 10% rubber seed of egg feedstuff total amount to not genetically modified chicken feed, and feed ingredient is done to corresponding adjustment, add the vitamin E of 5 international units in per kilogram feed, feedstuff composition and each component content are:
By above-mentioned chicken feed, feed the laying hen to the sea blue grey kind of 130～140 ages in days, employing is raised in cages, and feed trough is fed, free diet, and standing water, feeding, after 2 weeks, obtains being rich in the egg of ω-3 series fatty acid.Through gas chromatograph-mass spectrometer (GC-MS), analyze, in each egg (edible portion), the content of ω-3 series fatty acid is respectively DHA:116.8mg, EPA:87.9mg,
102.1mg, total content is 306.8mg.
Embodiment 2
15% rubber seed of egg feedstuff total amount is added in non-transgenic egg feedstuff, and feed is done to corresponding adjustment, add the vitamin E of 5 international units in per kilogram feed, feedstuff composition and each component content are:
| Composition | Content (%) |
| Corn | 42.89 |
| Dregs of beans | 27.09 |
| Wheat bran | 0.59 |
| Lard | 2.66 |
| Rubber seed | 15．00 |
| Salt | 0.35 |
| Stone flour | 8.38 |
| Calcium monohydrogen phosphate | 1.80 |
| Lysine | 0.10 |
| Methionine | 0.14 |
| Laying hen premix | 1.00 |
By above-mentioned chicken feed, feed the laying hen to the sea blue grey kind of 130～140 ages in days, employing is raised in cages, and feed trough is fed, free diet, and standing water, feeding, after 3 weeks, obtains being rich in the egg of ω-3 series fatty acid.Through gas chromatograph-mass spectrometer (GC-MS), analyze, the content of analyzing ω-3 series fatty acid in each egg (edible portion) is respectively DHA:149.4mg, EPA:101.4mg,
107.2, total content is 358mg.
Embodiment 3:
Add 20% rubber seed of egg feedstuff total amount in non-transgenic egg feedstuff, and feed is done to corresponding adjustment, add the vitamin E of 5 international units in per kilogram feed, feedstuff composition and each component content are:
| Composition | Content (%) |
| Corn | 42.89 |
| Dregs of beans | 22.25 |
| Wheat bran | 0.59 |
| Lard | 2.5 |
| Rubber seed | 20.00 |
| Salt | 0.35 |
| Stone flour | 8.38 |
| Calcium monohydrogen phosphate | 1.80 |
| Lysine | 0.10 |
| Methionine | 0.14 |
| Laying hen premix | 1.00 |
By above-mentioned chicken feed, feed the laying hen to 130～140 ages in days, employing is raised in cages, and feed trough is fed, free diet, and standing water, feeding, after 4 weeks, obtains being rich in the egg of ω-3 series fatty acid.Through gas chromatograph-mass spectrometer (GC-MS), analyze, analyze the content of ω-3 series fatty acid in each egg (edible portion) respectively: DHA:225.4mg, EPA:156.5mg,
110.9mg, total content is 492.8mg.
Embodiment 4
Add 15% rubber seed oil cake of egg feedstuff total amount in not genetically modified chicken feed, and its feed ingredient is done to corresponding adjustment, add the vitamin E of 5 international units in per kilogram feed, feedstuff composition and each component content are:
| Composition | Content (%) |
| Corn | 42.89 |
| Dregs of beans | 27.06 |
| Wheat bran | 0.59 |
| Lard | 2.69 |
| Rubber seed oil cake | 15.00 |
| Salt | 0.35 |
| Stone flour | 8.38 |
| Calcium monohydrogen phosphate | 1.80 |
| Lysine | 0.10 |
| Methionine | 0.14 |
| Laying hen premix | 1.00 |
By above-mentioned chicken feed, feed the laying hen to the sea blue grey kind of 130～140 ages in days, employing is raised in cages, and feed trough is fed, free diet, and standing water, feeding, after 2 weeks, obtains being rich in the egg of ω-3 series fatty acid.Through gas chromatograph-mass spectrometer (GC-MS), analyze, in each egg (edible portion), the content of ω-3 series fatty acid is respectively DHA:104.3mg, EPA:84.8mg,
99.1mg, total content is: 288.3mg.
Embodiment 5
Add 20% rubber seed oil cake of egg feedstuff total amount in not genetically modified chicken feed, and its feed ingredient is done to corresponding adjustment, add the vitamin E of 5 international units in per kilogram feed, feedstuff composition and each component content are:
| Composition | Content (%) |
| Corn | 42.89 |
| Dregs of beans | 22.23 |
| Wheat bran | 0.59 |
| Lard | 2.52 |
| Rubber seed oil cake | 20.00 |
| Salt | 0.35 |
| Stone flour | 8.38 |
| Calcium monohydrogen phosphate | 1.80 |
| Lysine | 0.10 |
| Methionine | 0.14 |
| Laying hen premix | 1.00 |
By above-mentioned chicken feed, feed the laying hen to the sea blue grey kind of 130～140 ages in days, employing is raised in cages, and feed trough is fed, free diet, and standing water, feeding, after 3 weeks, obtains being rich in the egg of ω-3 series fatty acid.Through gas chromatograph-mass spectrometer (GC-MS), analyze, in each egg (edible portion), the content of ω-3 series fatty acid is respectively DHA:132.7mg, EPA:91.8mg,
104.1mg, total content is: 328.6mg.
embodiment 6
Add 25% rubber seed oil cake of egg feedstuff total amount in not genetically modified chicken feed, and its feed ingredient is done to corresponding adjustment, add the vitamin E of 5 international units in per kilogram feed, feedstuff composition and each component content are:
| Composition | Content (%) |
| Corn | 42.89 |
| Dregs of beans | 17.41 |
| Wheat bran | 0.59 |
| Lard | 2.34 |
| Rubber seed oil cake | 25.00 |
| Salt | 0.35 |
| Stone flour | 8.38 |
| Calcium monohydrogen phosphate | 1.80 |
| Lysine | 0.10 |
| Methionine | 0.14 |
| Laying hen premix | 1.00 |
By above-mentioned chicken feed, feed the laying hen to the sea blue grey kind of 130～140 ages in days, employing is raised in cages, and feed trough is fed, free diet, and standing water, feeding, after 4 weeks, obtains being rich in the egg of ω-3 series fatty acid.Through gas chromatograph-mass spectrometer (GC-MS), analyze, in each egg (edible portion), the content of ω-3 series fatty acid is respectively DHA:207.2mg, EPA:126.9mg,
108.1mg, total content is: 442.2mg.
Claims (2)
1. be rich in a preparation method for the serial polyunsaturated fatty acid egg in ω-3, it is characterized in that carrying out according to the following steps:
(1) rubber seed of screening or rubber seed oil cake are added in non-transgenic egg feedstuff, rubber seed adds by 10～20% of egg feedstuff total amount, and rubber seed oil cake adds by 15～25% of egg feedstuff total amount;
(2) adjust feed composition, make composition consist of % by weight: corn 42.89, dregs of beans 17～32, wheat bran 0.59, lard 2～3, rubber seed 10～20 or rubber seed oil cake 15～25, salt 0.35, stone flour 8.38, calcium monohydrogen phosphate 1.8, lysine 0.1, methionine 0.14, laying hen premix 1, in per kilogram feed, the content of vitamin E is 5 international units;
(3) with above-mentioned feed, feed the laying hen of 130～140 ages in days, feed the egg that 2～4 weeks can output be rich in the serial polyunsaturated fatty acid in ω-3.
One kind by the preparation method of the serial polyunsaturated fatty acid egg in the ω of being rich in-3 claimed in claim 1, prepare be rich in the serial polyunsaturated fatty acid egg in ω-3.
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Cited By (1)
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|---|---|---|---|---|
| CN106858141A (en) * | 2017-03-06 | 2017-06-20 | 北京大伟嘉生物技术股份有限公司 | A kind of feed produced rich in the polyunsaturated fatty acids of ω 3 and Co-Q10 egg |
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| CN106858141A (en) * | 2017-03-06 | 2017-06-20 | 北京大伟嘉生物技术股份有限公司 | A kind of feed produced rich in the polyunsaturated fatty acids of ω 3 and Co-Q10 egg |
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