CN102824278A - Cosmetic compostion for preventing skin aging - Google Patents
Cosmetic compostion for preventing skin aging Download PDFInfo
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- CN102824278A CN102824278A CN2011103517374A CN201110351737A CN102824278A CN 102824278 A CN102824278 A CN 102824278A CN 2011103517374 A CN2011103517374 A CN 2011103517374A CN 201110351737 A CN201110351737 A CN 201110351737A CN 102824278 A CN102824278 A CN 102824278A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0241—Containing particulates characterized by their shape and/or structure
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/413—Nanosized, i.e. having sizes below 100 nm
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/60—Particulates further characterized by their structure or composition
- A61K2800/61—Surface treated
- A61K2800/612—By organic compounds
Abstract
The present invention relates to a cosmetic composition for preventing skin aging containing, as active ingredients, gold nanoparticles which have been surface-treated with a mixed solution which has one organic acid, selected from gallic acid and protocatechuic acid, and which has isoflavones. The gold nanoparticles (GI-Au NPs, PI-AU NPs) which have been surface-treated with phytochemicals according to the present invention exhibit no cytotoxicity, have a superior antioxidant effect, have a superior skin-regenerating effect due to the suppression of skin cell collagen cleaving enzymes (MMP-1 and MMP-8), and have a superior superoxide dismutase (SOD) activity and thus a superior degree of antioxidation in skin cells, and therefore may be useful as a cosmetic composition for preventing skin aging.
Description
Technical field
The present invention relates to pass through the cosmetic composition that is used to prevent skin aging that the plain surface-treated golden nanometer particle of phytochemistry contains as effective ingredient, relate in particular to carrying out the cosmetic composition that is used to prevent skin aging that the surface-treated golden nanometer particle contains as effective ingredient through a kind of organic acid of selection from gallic acid and protocatechuic acid (proto Catechin) and the mixed solution of isoflavone.
Background technology
Main cause as skin aging can be enumerated sunlight and active oxygen.Active oxygen is to be the material that plays a kind of like this effect, that is, in removing the process that gets into intravital foreign body, decompose foreign body and remove foreign body.Though play a positive role,, and combine with intravital unsaturated fatty acid and form lipid peroxide if, not only encroach on the cell tissue of self because a variety of causes produces too much active oxygen.Especially, in skin, combine and generate oxide, thereby promote skin aging, and melanic oxidizing process is played stronger effect, cause pigmentation with protein, lipid.
Usually, active oxygen is a kind of also breakneck material of cause cancer of skin aging that not only causes, and this is well-known.The method of removing this active oxygen has multiple, wherein uses a kind of utilization to be called the method for the plain biological active substances that is contained in plant of phytochemistry recently mostly.Biological active substances in the material that from this phytochemistry element, extracts mainly contains organic acid and Polyphenols etc.
Gallic acid as a kind of organic acid in the phytochemistry element mainly comprises in the material such as fruit shell, acorn nut, Pedicellus et Pericarpium Trapae (Water Chestnut), Fructus Schisandrae Chinensis, Galla Chinensis etc.; This gallic acid is the structure with three hydroxyls; Chemistry 3,4,5 trihydroxybenzoic acids by name.This material is excellent aspect antioxidant effect, and has such as characteristics such as anti-inflammatory, mutation, antiallergic effects.
In addition; Protocatechuic acid (proto catechin) as another kind of organic acid in the phytochemistry element mainly comprises in plants such as Radix Paeoniae (peony), Fructus Schisandrae Chinensis, Cortex Acanthopancis; This protocatechuic acid has the structure of two hydroxyls; Chemistry is called 2, and the 3-resorcylic acid also has multiple physiologically active.Especially have characteristics such as resisting blood coagulation, anti-inflammatory, antioxidant effect.
In addition, if, then mainly in Semen sojae atricolor, comprise, and mainly be present in the plumule in the Semen sojae atricolor as the isoflavone of Polyphenols in the phytochemistry element.Its structure of isoflavone and active aspect and estrogen (estrogen) are similar, therefore also are known as phytoestrogen (phytoestrogen).Isoflavone structurally belongs to flavonoid (flavonoid), and the isoflavone that contains sugar is known as glucoside (glucoside), and the isoflavone that does not contain sugar is known as aglycone (aglycone).In some report, write the result of study that isoflavone can prevent breast carcinoma, prostatitis, uterus carcinoma, colorectal cancer, cardiovascular disease, osteoporosis.
In addition, proof gold (Au; Gold) owing to have biocompatibility (biocompatibility), therefore use widely on medical science and the western medicine in the Orient.According to putting down in writing in the Dong-eui-bo-gam; Proof gold can be kept the ionic equilibrium of stream fine electric current in vivo; And have the Detoxication of calm effect and slop, and make qi and blood circulation smooth and easy, the gold that especially is applied in skin is through particle effect blood circulation promoting.And, if utilize gold, then promote hormone secretion and prevent to generate wrinkle, and prevent dermatosis also have the effect of calming the nerves etc. through detoxifcation and purification.And, know that also gold can remove residue, and have the effect of various adult diseases such as prevention of arterial sclerosis aspect aging resistance, also to have certain effect.Be used for asthma or rheumatismal treatment to gold in the western medicine.As stated, gold utensil has biological and multiple physiologically active, is therefore developing medicine or the cosmetics that utilize gold widely, but also is being used as CT contrast agent and thermotherapy agent.
Up to the present; Though exploitation has the medicine that utilizes golden nanometer particle or cosmetics etc. widely; But; About utilizing the cosmetic composition that skin aging prevents that is used for through the phytochemistry element surface-treated golden nanometer particle of gallic acid and isoflavone or protocatechuic acid and isoflavone etc., also do not known by people, also have no talent and this was carried out research.
Summary of the invention
Technical problem
The present inventor has confirmed following situation in utilizing the Research on effect process that whether has the skin aging of preventing through phytochemistries such as gallic acid, protocatechuic acid, isoflavone element surface-treated golden nanometer particle.That is, utilize the stronger antioxidant properties of gallic acid and isoflavone or protocatechuic acid and isoflavone to induce gold chloride (HAuCl
4) reduction and the golden nanometer particle that makes does not have cytotoxicity; And antioxidant effect is excellent; And owing to suppress collagen protein (collagen) catabolic enzyme, so the skin regeneration ability is excellent, and because superoxide dismutase (SOD) superior activity; Therefore Skin Cell antioxidation degree is excellent, has accomplished the present invention in view of the above.
Therefore, the present invention provides a kind of cosmetic composition that is used to prevent skin aging that will utilize the mixed solution surface-treated golden nanometer particle through a kind of organic acid selected in gallic acid and the protocatechuic acid and isoflavone to contain as effective ingredient.
Technical scheme
The present invention provides a kind of cosmetic composition that is used to prevent skin aging that will utilize the mixed solution surface-treated golden nanometer particle through a kind of organic acid selected in gallic acid and the protocatechuic acid and isoflavone to contain as effective ingredient.
Below, the present invention is elaborated.
The cosmetic composition that is used for preventing skin aging of the present invention as effective ingredient, be characterised in that with the surface treated golden nanometer particle of protocatechuic acid, utilize the resistance to oxidation of the mixed solution of a kind of organic acid selected in gallic acid and the protocatechuic acid and isoflavone to induce gold chloride (HAuCl
4) reduction, carry out surface treatment thus.
Specifically; Be dissolved into distilled water through gold chloride and prepare chlorauric acid solution 0.01mmol to 0.05mmol; And preparation is dissolved into the glucoside isoflavone mixed solution of a kind of organic acid soln of from the gallic acid of 0.01mol/L to 0.03mol/L and protocatechuic acid, selecting; This mixed solution is joined said chlorauric acid solution and stirring, prepare golden nanometer particle (GI-Au NPs, PI-Au NPs) thus.
Can further comprise the step that prepared said golden nanometer particle is coated with biocompatibility macromolecule solution.Said biocompatibility macromolecule is preferably Polyethylene Glycol, and (polyethylene glycol PEG), but is not limited thereto, and can realize so long as have the macromolecule of biocompatibility.Said macromolecular solution is preferably the macromolecular solution that uses 1%～4% (w/v), and the volumetric ratio of golden nanometer particle and macromolecular solution is preferably 1 during coating: (2～4).
With plain surface treated golden nanometer particle (the GI-Au NPs of phytochemistry provided by the present invention; PI-Au NPs) no cytotoxicity; Antioxidant effect is excellent; And the skin regeneration ability is excellent through the collagen protein catabolic enzyme (MMP-1 and MMP-8) that suppresses Skin Cell, and because superoxide dismutase (SOD) superior activity, thereby Skin Cell antioxidation degree is excellent.Therefore, can effectively be applicable to the cosmetic composition that is used to prevent skin aging with the plain surface treated golden nanometer particle of phytochemistry provided by the present invention (GI-Au NPs, PI-Au NPs).
Cosmetic composition of the present invention can contain more than one with the known effective ingredient that together has antioxidant activity with the plain surface treated golden nanometer particle of phytochemistry (GI-Au NPs, PI-Au NPs).
And compositions of the present invention can also further comprise the carrier that allows on more than one the cosmetic conduct and learning except the effective ingredient of above-mentioned record, to be applicable to the preparation of the cosmetics of common manufacturing in this area.Specifically; Comprise fatty material, organic solvent, lytic agent, concentrating agents, gellant, softening agent, antioxidant, suspension, stabilizing agent, foaming agent, aromatic, surfactant, water, ion-type or nonionic emulsifier, filler, metal ion masking agent (sequestering agent) and chelating agen, antiseptic, vitamin, blocking agent, wetting agent, essential oil, dyestuff, pigment, hydrophilic or the lipophile activating agent that uses, lipid vesicle or use usually other compositions arbitrarily on the cosmetics and in cosmetic field normally used adjuvant; Thus applicable to solution, suspension, emulsion (emulsion), cream (paste), gluey (gel); (wax foundation) and aerosol apparatus etc. at the bottom of cream (cream), washing liquid, face powder, soap, the makeup removing oil that contains surfactant, foundation cream, emulsion foundation cream, the wax, but be not limited thereto.Further explain can be prepared into the preparation that is applicable to soft astringent, nutrition astringent, nutritional emulsions, nourishing cream, massage cream, essence, eye cream, makeup removing frost, cleansing milk, clean water, facial film, aerosol apparatus, face powder.
In cosmetic composition of the present invention; With plain surface treated golden nanometer particle (the GI-Au NPs of phytochemistry; PI-Au NPs) content is 0.0001 weight %～20 weight % with respect to the cosmetic composition gross weight, is preferably to comprise 0.001 weight %～5 weight %.If the content of golden nanometer particle is less than 0.0001 weight %, then be difficult to show the effect that prevents skin aging, if surpass 20 weight %, then cause the possibility of skin irritation height, also may produce bigger influence to the stabilisation of dosage form.
The invention effect
Provided by the invention with plain surface treated golden nanometer particle (the GI-Au NPs of phytochemistry; PI-Au NPs) there is not cytotoxicity; And antioxidant effect is excellent; And the skin regeneration ability is excellent owing to suppress the collagen protein catabolic enzyme (MMP-1 and MMP-8) of Skin Cell, and because the superior activity of superoxide dismutase (SOD) and the antioxidation degree of Skin Cell is excellent, so can effectively be applicable to the cosmetic composition that is used to prevent skin aging.
Description of drawings
Fig. 1 is for illustrating the figure [(A) GI-Au NPs, (B) GI-Au NPs] based on the survival rate of the L-929 cell of the concentration (0～50 μ g/ml) of golden nanometer particle provided by the present invention (GI-Au NPs, PI-Au NPs) and natural law (a day, three days, five days, seven days);
Fig. 2 observes the figure of the L-929 cell of handling through the golden nanometer particle provided by the present invention (GI-Au NPs, PI-Au NPs) of 25 μ g/ml and 50 μ g/ml for utilizing scanning electron microscope (SEM);
Fig. 3 observes the figure of the L-929 cell of handling through the golden nanometer particle provided by the present invention (GI-Au NPs, PI-Au NPs) of 25 μ g/ml and 50 μ g/ml for utilizing transmission electron microscope (TEM);
Fig. 4 a is golden nanometer particle of the present invention (the GI-Au NPs that illustrates to through the L-929 cell of the hydrogen peroxide treatment of 200 μ mol/L; The mensuration result's of the antioxidant effect (B) of antioxidant effect PI-Au NPs) (A, inserted what illustrate is antioxidant effect mechanism) and antioxidase (catalase (catalase)) figure;
Fig. 4 b for to through the antioxidant effect of the plain chemical compound of phytochemistry of the L-929 cell of the hydrogen peroxide treatment of 200 μ mol/L ((A): GA, IF, PCA, (B): PI, mensuration result's GI) figure;
Fig. 5 is through golden nanometer particle of the present invention (GI-Au NPs with fluorescence microscope; PI-Au NPs) and the figure of the L-929 cell of the hydrogen peroxide treatment of 200 μ mol/L [(A) for matched group (being untreated); (B) be the hydrogen peroxide treatment of 200 μ mol/L; (C) be golden nanometer particle (GI-Au NPs) processing of 25 μ g/ml; (D) being golden nanometer particle (PI-Au NPs) processing of 25 μ g/ml, (E) being the hydrogen peroxide treatment of the GI-Au NPs+200 μ mol/L of 25 μ g/ml, (F) is the hydrogen peroxide treatment of the PI-Au NPs+200 μ mol/L of 25 μ g/ml];
Fig. 6 is with the result's (A) of the epidermis of the hairless mouse of observation by light microscope process golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs) processing and corium the figure and the block diagram (B) of epidermis and dermis thickness;
Fig. 7 a is for confirming through golden nanometer particle of the present invention (GI-Au NPs through immunoblotting; Skin histology matrix metalloproteinase (the MMPs) (MMP-1 of the hairless mouse of PI-Au NPs) handling; MMP-8 and MMP-9) figure of protein expression degree (expression); Fig. 7 b is for confirming through immunoblotting through the plain chemical compound of phytochemistry (GI, the figure of the expression degree of MMP-1 PI) and in the hairless mouse skin tissue handled of the cosmetics of on market, selling;
Fig. 8 is the active figure of superoxide dismutase (SOD) in the Skin Cell of the hairless mouse that golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs) is handled.
The specific embodiment
Below, in order to help to understand the present invention preferred embodiment is provided.But following embodiment has been merely and has understood the present invention with being more prone to and provide, and content of the present invention is not limited thereto.
Preparation example one: through the preparation (GI-Au NPs) of gallic acid and isoflavone surface-treated golden nanometer particle
The present inventor utilizes the method for being put down in writing among the PCT/KR/2010/007516 that had applied for to prepare golden nanometer particle.Specifically, with gold chloride (HAuCl
4) (7.8mg 0.02mmol) is dissolved in the distilled water of 20ml, has prepared gold chloride thus.Then, preparation has been dissolved the mixed solution of the glucoside isoflavone of 10mg to the gallic acid solution of 0.01mol/L, again after, this mixed solution of 0.3ml is joined in the chlorauric acid solution, and stirred 30 minutes, prepared golden nanometer particle thus.
Preparation example two: with the preparation (PI-Au NPs) of protocatechuic acid and the surface treated golden nanometer particle of isoflavone
With gold chloride (HAuCl
4) (7.8mg 0.02mmol) is dissolved in the distilled water of 20ml, has prepared gold chloride thus.Then, preparation has been dissolved the mixed solution of the isoflavone of 10mg to the protocatechuic acid solution of 0.01mol/L, again after, this mixed solution of 0.3ml is joined in the said chlorauric acid solution, and stirred 30 minutes, prepared golden nanometer particle thus.
Embodiment one: the CTA of golden nanometer particle
In order to confirm the cytotoxicity of golden nanometer particle provided by the present invention, utilize a kind of L-929 cell as muscle cell, carried out following test through WST-8.
Specifically, join respectively in the cell culture district (well) with L-929 cell with the golden nanometer particle (GI-Au NPs, PI-Au NPs) for preparing 0～50ug/ml of preparation in the example two, cultivated seven days in said preparation example one.After the cultivation, utilize immunodetection (ELISA) to measure the absorbance at 450nm place, confirmed cell survival rate thus.And, the L-929 cell of handling through the golden nanometer particle (GI-Au NPs, PI-Au NPs) of 25 μ g/ml and 50 μ g/ml that utilized scanning electron microscope and transmission electron microscope observation.
Fig. 1 shows concentration based on golden nanometer particle provided by the present invention (0～50ug/ml) and natural law (a day; Three days; Five days, seven days) the survival rate [(A) being GI-Au NPs, (B) is PI-Au NPs] of L-929 cell; Fig. 2 and Fig. 3 show respectively and utilize scanning electron microscope (SEM) and the transmission electron microscope observation result through the treated L-929 cell of golden nanometer particle provided by the present invention (GI-Au NPs, PI-Au NPs) of 25 μ g/ml and 50 μ g/ml.
As shown in Figure 1, the cell survival rate of golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs) (has shown in 0～50ug/ml) more than 90%, has compared with matched group and also almost do not show difference in all concentration.Therefore, can learn that golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs) does not almost have cytotoxicity.
And, as shown in Figure 2, confirm no matter golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs) attaches to cell surface or is penetrated into the cell interior cell still survives.
And, as shown in Figure 3, confirm golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs) and entered in the cell fully.Therefore, golden nanometer particle of the present invention also can be used as the drug delivery body and uses effectively.
Embodiment two: the antioxidant effect of golden nanometer particle is measured
In order to confirm the antioxidant effect of golden nanometer particle provided by the present invention, utilize people's active oxygen (ROS, reactve oxygen species) test kit (assay kit) to carry out following test.
Specifically, include respectively on the culture medium of the golden nanometer particle (GI-Au NPs, PI-Au NPs) of 0～50ug/ml of preparation in said preparation example one and preparation example two and cultivated the L-929 cell one day.Handle with the hydrogen peroxide of 200 μ mol/L then.Afterwards, be used as 2 of fluorophor probe ', 7 '-the two hydrogen fluorescein(e) diacetates of dichloro (2 ', 7 '-dichlorodihydrofluorescein diacetate; DCFH-DA) said L-929 cell is handled, make 2 ', 7 '-the two hydrogen fluorescein(e) diacetates of dichloro are penetrated in the cell.At this moment, because hydrogen peroxide, DCFH-DA takes place deacetylated, and has fluorescence, when golden nanometer particle is removed hydrogen peroxide, be included in intracellular DCFH-DA can not carry out deacetylated, thereby can not give expression to fluorescence.With the fluorescence of not expressing is that benchmark has been measured antioxidant effect.Then, with fluorescence microscope the L-929 cell.
And, utilize concentration identical and identical method with above-mentioned antioxidant effect assay method, measured plain chemical compound (protocatechuic acid (the Protocatechuic acid of phytochemistry; PCA), and isoflavone (isoflavone, IF); Gallic acid (gallic acid; GA), PI, GI) inherent antioxidant effect.
Fig. 4 shows to golden nanometer particle of the present invention (the GI-Au NPs through the L-929 cell of the hydrogen peroxide treatment of 200 μ mol/L; PI-Au NPs) antioxidant effect (A; Inserted what illustrate is antioxidant effect mechanism) and the mensuration result of the antioxidant effect (B) of antioxidase (catalase (catalase)); Shown in Fig. 5 is process golden nanometer particle of the present invention (the GI-Au NPs that arrives with fluorescence microscope; PI-Au NPs) and the result of the L-929 cell of the hydrogen peroxide treatment of 200 μ mol/L [(A) for matched group (being untreated); (B) being the hydrogen peroxide treatment of 200 μ mol/L, (C) is that the golden nanometer particle (GI-Au NPs) of 25 μ g/ml is handled, and (D) is that the golden nanometer particle (PI-Au NPs) of 25 μ g/ml is handled; (E) being the hydrogen peroxide treatment of the GI-Au NPs+200 μ mol/L of 25 μ g/ml, (F) is the hydrogen peroxide treatment of the PI-Au NPs+200 μ mol/L of 25 μ g/ml].
Shown in Fig. 4 a; When with the golden nanometer particle (PI-Au NPs) of about 7 μ g/ml when the L-929 cell is handled; Confirming hydrogen peroxide has reduced more than 25%; When the L-929 cell is handled, confirming hydrogen peroxide and reduced more than 30% with the golden nanometer particle (PI-Au NPs) of about 10 μ g/ml.And,, find that hydrogen peroxide has reduced more than 40% when the golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs) of whole usefulness 50 μ g/ml when the L-929 cell is handled.This is considered to have about 3,000 units (unit) the similar activity in the left and right sides with antioxidase (catalase (catalase)).
And; Shown in Fig. 4 b; The result the who respectively antioxidant effect of the plain chemical compound of phytochemistry is confirmed; Confirm if the GA of 50 μ g/ml then shows the antioxidant effect about 10%, if then show 20% antioxidant effect when IF, PCA, PI (PCA+IF) and GI (GA+IF).Therefore; When handling cell with the golden nanometer particle of 50 μ g/ml; If compare with the result of the above-mentioned Fig. 4 a that shows about 40% antioxidant effect, then can find the better excellence of antioxidant effect of golden nanometer particle provided by the present invention (GI-Au NPs, PI-Au NPs).
And, as shown in Figure 5, (A) in matched group (being untreated), do not observe the cell that has fluorescence fully.But, (B) in the hydrogen peroxide treatment group, observed the cell that much has fluorescence, this representes that most cell is oxidized and has received damage.And, (C, D) in golden nanometer particle of the present invention (GI-Au NPs, the PI-Au NPs) processed group with matched group in situation equally do not observe the cell that has fluorescence fully.And (E F) is carrying out with golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs) in the group that the reuse hydrogen peroxide is handled after the pre-treatment, and the quantity of confirming to have the cell of fluorescence reduces significantly.
Can know that thus the antioxidant effect of golden nanometer particle provided by the present invention (GI-Au NPs, PI-Au NPs) is excellent.
Embodiment three: the Skin Cell regeneration capacity of golden nanometer particle is measured
For the Skin Cell regeneration capacity of golden nanometer particle provided by the present invention, carried out following test.
Specifically, with ten the week ages 35 of hairless mouses (hairless mice) be divided into each the group five, be divided into seven groups thus.Specific part to hairless mouse skin in each group is handled with golden nanometer particle (GI-Au NPs, PI-Au NPs) and the distilled water of the 3 μ g/ml that prepare preparation in the example two, 12 μ g/ml, 50 μ g/ml with the said preparation example one of 50 μ l respectively.For golden nanometer particle, the inherent identical time of every day of one month time handles the skin of hairless mouse by number of times once a day, finishes then after the test, carries out having obtained skin after the carbon dioxide narcosis.The skin histology of being obtained is taken care of under the condition below-70 ℃ till carrying out biopsy and protein analysis.
With observation by light microscope the skin histology (epidermis of hairless mouse; Corium) variation; And confirmed skin histology matrix metalloproteinase (MMPs) (MMP-1, MMP-8 and MMP-9) the protein expression degree (expression) of hairless mouse through immunoblotting.MMP-1 and MMP-8 represent the collagen protein catabolic enzyme of skin among the MMPs, and MMP-9 representes animal glue (gelatin) catabolic enzyme of skin.
And; In order to understand hairless mouse to the plain chemical compound (GI of phytochemistry through concentration identical and identical method with above-mentioned method; PI) and the Skin Cell regeneration effect of the cosmetics of the L company of on market, selling, confirmed expression degree as the MMP-1 of collagen protein catabolic enzyme.
Fig. 6 shows with observation by light microscope through golden nanometer particle of the present invention (GI-Au NPs; Epidermis and the result (A) of corium and the block diagram (B) of epidermis and dermis thickness of the hairless mouse skin of PI-Au NPs) handling; Fig. 7 a shows through immunoblotting and confirms matrix metalloproteinase (MMPs) (MMP-1; MMP-8 and MMP-9) result of protein expression degree, Fig. 7 b shows through immunoblotting and confirms the result to the expression degree of the MMP-1 of the cosmetics of plain chemical compound of phytochemistry and L company.
As shown in Figure 6, observe the epidermis of the hairless mouse skin of handling through golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs) and the thickness of corium and compared the matched group thickening.Can know that thus golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs) is excellent aspect the Skin Cell regeneration capacity.
And, shown in Fig. 7 a, can confirm that the expression that MMPs is suppressed by golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs).Specifically; For MMP-1, confirm it and express owing to golden nanometer particle (PI-Au NPs) is suppressed, but because the golden nanometer particle (GI-Au NPs) of 12 μ g/ml and 50 μ g/ml; It is expressed and is significantly reduced; For MMP-8, to confirm owing to golden nanometer particle (PI-Au NPs), its expression is reduced.But for MMP-9, because golden nanometer particle (GI-Au NPs, PI-Au NPs), its expression is not reduced.
In addition; Shown in Fig. 7 b; Can confirm that (GI PI) with the cosmetics of L company, is not suppressed as the expression of the MMP-1 of phytochemistry catabolic enzyme for the plain chemical compound of phytochemistry; If compare with the situation that the expression of MMP-1 is suppressed by golden nanometer particle provided by the present invention this moment, then can judge through the plain chemical compound surface-treated of phytochemistry golden nanometer particle and can further improve the skin regeneration effect.
Can know thus; Golden nanometer particle of the present invention (GI-Au NPs; PI-Au NPs) reduces the phenomenon that the collagen protein of skin is decomposed through the expression that suppresses MMP-1 and MMP-8 (collagen protein catabolic enzyme), and measurablely go out skin regeneration is had desirable influence.
Embodiment 4: the Skin Cell antioxidation degree of golden nanometer particle is measured
In order to confirm the Skin Cell antioxidation degree of golden nanometer particle provided by the present invention; Utilize superoxide dismutase (SOD) test kit (assay kit)-WST to measure superoxide dismutase (superoxide dismutase, activity SOD) through colorimetric analysis.Specifically, the skin cell tissue through refrigerated hairless mouse of 100mg is immersed in sucrose buffer solution (sucrose buffer solution) and afterwards, has measured the absorbance of mixed solution, and through relatively analyzing with blank solution.
Fig. 8 shows this result.
As shown in Figure 8; The activity of the superoxide dismutase of golden nanometer particle of the present invention in the Skin Cell of hairless mouse (PI-Au NPs) is very excellent; For golden nanometer particle (GI-Au Nps), the activity of superoxide dismutase is comparatively excellent when the concentration of using 12 μ g/ml is handled.Hence one can see that, and golden nanometer particle of the present invention (GI-Au NPs, PI-Au NPs) is excellent aspect Skin Cell antioxidation degree.
Below, enumerate the formulation example of cosmetic composition of the present invention.
Formulation example one: the preparation of cosmetic formulations
1, the preparation of soft astringent
[table]
| Composition | Content (weight %) |
| Golden nanometer particle (GI-Au NPs or PI-Au NPs) | 0.5 |
| 1,3 butylene glycol | 5.2 |
| Oleyl alcohol (Oleyl Alcohol) | 1.5 |
| Ethanol (ethanol) | 3.2 |
| Polysorbate20 (Polysorbate 20) | 3.2 |
| Benzophenone-9 (Benzophenone-9) | 2.0 |
| CVP Carbopol ETD2050 (Carboxy vinyl polymer) | 1.0 |
| Glycerol | 3.5 |
| Spice | Trace |
| Antiseptic | Trace |
| Purified Water | Surplus |
| Add up to | 100 |
After mentioned component mixed, prepare according to the method for preparing of common soft astringent.
2, the preparation of nutritional emulsions
[table two]
| Composition | Content (weight %) |
| Golden nanometer particle (GI-Au NPs or PI-Au NPs) | 0.6 |
| Glycerol | 5.1 |
| Propylene glycol (Propylene Glycol) | 4.2 |
| Tocopherol acetas (Tocopheryl Acetate) | 3.0 |
| Liquid paraffin (liquid paraffin) | 4.6 |
| Triethanolamine | 1.0 |
| Squalane (Squalane) | 3.1 |
| Australia Oleum Juglandis (Macadamia Nut Oil) | 2.5 |
| Polysorbate60 | 1.6 |
| Sorbitan Sesquioleate (Sorbitan Sesquioleate) | 1.6 |
| Propylparaben (Propyl paraben) | 0.6 |
| CVP Carbopol ETD2050 (Carboxy vinyl polymer) | 1.5 |
| Spice | Trace |
| Antiseptic | Trace |
| Purified Water | Surplus |
| Add up to | 100 |
After mentioned component mixed, prepare according to the method for preparing of common nutritional emulsions.
3, the preparation of nourishing cream
[table three]
| Composition | Content (weight %) |
| Golden nanometer particle (GI-Au NPs or PI-Au NPs) | 1.0 |
| Glycerol | 4.0 |
| Vaseline | 3.5 |
| Triethanolamine | 2.1 |
| Liquid paraffin (liquid paraffin) | 5.3 |
| Squalane (Squalane) | 3.0 |
| Cera Flava (beeswax) | 2.6 |
| Tocopherol acetas (Tocopheryl Acetate) | 5.4 |
| Polysorbate60 | 3.2 |
| CVP Carbopol ETD2050 (Carboxy vinyl polymer) | 1.0 |
| Sorbitan Sesquioleate (Sorbitan Sesquioleate) | 3.1 |
| Spice | Trace |
| Antiseptic | Trace |
| Purified Water | Surplus |
| Add up to | 100 |
After mentioned component mixed, prepare according to the method for preparing of common nourishing cream.
Claims (4)
1. a cosmetic composition that is used to prevent skin aging is characterized in that, the surface treated golden nanometer particle of mixed solution that will use a kind of organic acid from gallic acid and protocatechuic acid, selected and isoflavone is as effective ingredient.
2. the cosmetic composition that is used to prevent skin aging as claimed in claim 1; It is characterized in that; Said golden nanometer particle is dissolved into the glucoside isoflavone after the mixed solution of organic acid soln through preparation chlorauric acid solution and preparation; Said mixed solution joined in the said chlorauric acid solution stir and make; Wherein said chlorauric acid solution is dissolved into distilled water through the gold chloride with 0.01mmol to 0.05mmol and makes, and said organic acid soln is a kind of organic acid soln of from the gallic acid of 0.01mol/L to 0.03mol/L and protocatechuic acid, selecting.
3. the cosmetic composition that is used to prevent skin aging as claimed in claim 1 is characterized in that, with respect to the cosmetic composition gross weight, the content of said golden nanometer particle is 0.0001 weight %～20 weight %.
4. the cosmetic composition that is used to prevent skin aging as claimed in claim 1; It is characterized in that said preparation of compositions becomes to be applicable to more than one the preparation of from the group that soft astringent, nutrition astringent, nutritional emulsions, nourishing cream, massage cream, essence, eye cream, makeup removing frost, cleansing milk, clean water, facial film, aerosol apparatus, face powder are formed, selecting.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020110058918A KR101333209B1 (en) | 2011-06-17 | 2011-06-17 | Cosmetic composition for preventing skin aging comprising surface-modified gold nanoparticle by phytochemical |
| KR10-2011-0058918 | 2011-06-17 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102824278A true CN102824278A (en) | 2012-12-19 |
| CN102824278B CN102824278B (en) | 2015-05-13 |
Family
ID=47327727
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110351737.4A Expired - Fee Related CN102824278B (en) | 2011-06-17 | 2011-11-09 | Cosmetic composition for preventing skin aging |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR101333209B1 (en) |
| CN (1) | CN102824278B (en) |
| WO (1) | WO2012173312A1 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109464314A (en) * | 2018-11-02 | 2019-03-15 | 蝶柔化妆品(浙江)有限公司 | Gold bullet moistens Face-protecting mask and preparation method thereof |
| CN112387980A (en) * | 2019-08-19 | 2021-02-23 | 近镒生技股份有限公司 | Nano gold particle, drug carrier containing nano gold particle, preparation method of nano gold particle and application of nano gold particle |
| CN115607666A (en) * | 2021-07-12 | 2023-01-17 | 南开大学 | Fluorescent probe for treating tumor by photodynamic therapy |
| WO2023077338A1 (en) * | 2021-11-04 | 2023-05-11 | 深圳市维琪科技股份有限公司 | Hexapeptide derivative and cosmetic composition or pharmaceutical composition and use thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014168424A1 (en) * | 2013-04-10 | 2014-10-16 | 주식회사 유엑스엔 | Soap or cosmetic composition which contains gold beads and method for producing soap and cosmetic using same |
| KR102094061B1 (en) * | 2017-09-18 | 2020-03-26 | 진명종 | Method of anti-wrinkle and Anti-aging cosmetics compositions using colloidal gold |
| ES2726000A1 (en) * | 2018-03-28 | 2019-10-01 | Infinitec Activos S L | Gold nanoparticles and palmitoyl pentapeptide-4 (Machine-translation by Google Translate, not legally binding) |
| TWI801618B (en) * | 2019-06-27 | 2023-05-11 | 晶碩光學股份有限公司 | Contact lens product having anti-oxidation function |
| FR3098713B1 (en) * | 2019-07-16 | 2022-04-29 | Torskal | GOLD NANOPARTICLES COMPRISING A PLANT EXTRACT AND THEIR COSMETIC USE |
| KR102318369B1 (en) * | 2019-11-15 | 2021-10-28 | 부산대학교 산학협력단 | Cosmetic composition for uv-screening comprising biocompatible nano-carrier |
| KR102445953B1 (en) | 2021-09-30 | 2022-09-22 | 주식회사 에이유랩스 | Manufacturing Method of Composition For Wrinkle Reduction Comprising Niacinamide Deposited With Gold Nanoparticle |
| KR102360781B1 (en) | 2021-09-30 | 2022-02-14 | (주)쥬넥스 | Hydrocolloid Patch For Alleviating Skin Inflammation |
| KR102445954B1 (en) | 2021-09-30 | 2022-09-22 | 주식회사 에이유랩스 | Manufacturing Method of Composition For Wrinkle Reduction Comprising Niacinamide Deposited With Gold Nanoparticle |
| KR102445951B1 (en) | 2021-09-30 | 2022-09-22 | 주식회사 에이유랩스 | Manufacturing Method of Emulsion Composition Comprising Niacinamide Deposited With Gold Nanoparticle |
| KR102445952B1 (en) | 2021-09-30 | 2022-09-22 | 주식회사 에이유랩스 | Manufacturing Method of Antioxidant Composition Comprising Niacinamide Deposited With Gold Nanoparticle |
| KR102445955B1 (en) | 2021-09-30 | 2022-09-22 | 주식회사 에이유랩스 | Manufacturing Method of Composition For Skin Regeneration Comprising Niacinamide Deposited With Gold Nanoparticle |
| KR102478642B1 (en) | 2022-04-14 | 2022-12-16 | 주식회사 에이유랩스 | Manufacuturing Method of Niacinamide Powder Comprising Gold Particle |
| KR102515749B1 (en) | 2022-07-13 | 2023-03-30 | 주식회사 에이유랩스 | Manufacturing Method of Gold particle-Comprising Niacinamide Colloidal Solution |
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| WO2003020226A2 (en) * | 2001-08-31 | 2003-03-13 | L'oreal S.A. | Cosmetic compositions comprising nanoparticles and processes for using the same |
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| CN100594944C (en) | 2003-10-30 | 2010-03-24 | 麦克内尔-Ppc股份有限公司 | Soft surface coating composition, method for decorating soft surface and absorbent articles comprising metal-loaded nanoparticles |
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- 2011-11-09 CN CN201110351737.4A patent/CN102824278B/en not_active Expired - Fee Related
- 2011-11-10 WO PCT/KR2011/008533 patent/WO2012173312A1/en active Application Filing
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| WO2003020226A2 (en) * | 2001-08-31 | 2003-03-13 | L'oreal S.A. | Cosmetic compositions comprising nanoparticles and processes for using the same |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109464314A (en) * | 2018-11-02 | 2019-03-15 | 蝶柔化妆品(浙江)有限公司 | Gold bullet moistens Face-protecting mask and preparation method thereof |
| CN112387980A (en) * | 2019-08-19 | 2021-02-23 | 近镒生技股份有限公司 | Nano gold particle, drug carrier containing nano gold particle, preparation method of nano gold particle and application of nano gold particle |
| CN115607666A (en) * | 2021-07-12 | 2023-01-17 | 南开大学 | Fluorescent probe for treating tumor by photodynamic therapy |
| WO2023077338A1 (en) * | 2021-11-04 | 2023-05-11 | 深圳市维琪科技股份有限公司 | Hexapeptide derivative and cosmetic composition or pharmaceutical composition and use thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012173312A1 (en) | 2012-12-20 |
| KR20120139229A (en) | 2012-12-27 |
| CN102824278B (en) | 2015-05-13 |
| KR101333209B1 (en) | 2013-11-26 |
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