CN114129476A

CN114129476A - Composition comprising vegetable fruit oil and vegetable seed oil and use thereof

Info

Publication number: CN114129476A
Application number: CN202111527643.8A
Authority: CN
Inventors: 殷庆飞; 张瑶瑶
Original assignee: Shanghai Jahwa United Co Ltd
Current assignee: Shanghai Jahwa United Co Ltd
Priority date: 2021-12-14
Filing date: 2021-12-14
Publication date: 2022-03-04
Also published as: WO2023109289A1

Abstract

The invention provides a composition containing vegetable oil, wherein the vegetable oil comprises vegetable fruit oil and vegetable seed oil, and the vegetable fruit oil is selected from the following group: shea butter, olive fruit oil, sweet almond oil, avocado fruit oil, sea buckthorn fruit oil, coconut fruit oil, etc.; wherein the plant seed oil is selected from the group consisting of: jojoba seed oil, safflower seed oil, meadowfoam seed oil, sunflower seed oil, rice bran oil, macadamia nut oil, castor oil and the like, wherein the weight ratio of the vegetable fruit oil to the vegetable seed oil is from 2:8 to 8:2, wherein the composition comprises at least 0.5 wt% of vegetable oil based on the total weight of the composition. The invention also relates to the application of the composition containing the vegetable oil and fat in enhancing the skin barrier function and/or in preparing medicines and/or skin external preparations for enhancing the skin barrier function.

Description

Composition comprising vegetable fruit oil and vegetable seed oil and use thereof

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to a composition containing plant fruit oil and plant seed oil and application of the composition in the aspect of skin barrier function.

Background

Skin barrier broadly includes physical barrier, pigment barrier, nerve barrier, and immune barrier. The skin barrier is mainly defined as a physical barrier, and the skin barrier is usually said to be a physical barrier. The physical barrier is composed of a sebum membrane, horny layer keratin, lipid, a sandwich structure, a brick wall structure, dermal mucopolysaccharide, mucopolysaccharide and the like, resists the entrance of external harmful substances, irritants and sunlight, and has the functions of moisturizing, regulating and resisting inflammation. The basic function of sebum is moistening and moisture-keeping, and meanwhile, the weak acidity of sebum can also play a certain role in resisting bacteria. Sebum forms a "sebum film" on the skin surface, which is a natural moisturizing cream for the skin. If the skin lacks sebum, the barrier function of the skin is weakened, the sebum contains triglyceride, free fatty acid, wax ester, squalene and other components, and a sebum film can be reconstructed on the surface of the skin by smearing moisturizing cream, so that external stimulation can be effectively isolated, and the loss of skin moisture can be prevented.

According to different sources and chemical components of the grease, the grease can be mainly divided into: vegetable oil and fat, animal oil and fat, mineral oil and synthetic oil and fat, the vegetable oil and fat is close to the structure of human skin fat membrane due to the skin-friendly texture, has good permeability, deeply permeates and nourishes the skin, and is popular with a plurality of skin care products. The vegetable oil and fat used for cosmetic mainly come from fruit, seed and embryo of plant, and part of the vegetable comes from other parts of plant such as leaf, bark, root, petal and stamen. The oils and fats containing plant fruits mainly include shea butter (Butyrospermum parkii) fruit fat (also called shea butter), olive (Olea europaea) fruit oil (also called olive oil), apricot (Prunus armeniaca) kernel oil (also called sweet almond oil), avocado (Persea gratisma) fruit oil, sea buckthorn (Hippophae rhamnoides) fruit oil, coconut (Cocos nucifera) fruit oil, fragrant Citrus (Citrus junos) fruit oil, fragrant lemon (Citrus aurantium bergamia) fruit oil, citron (Citrus bica monolithium) fruit oil, tomato (Solanum lycopersicum) fruit oil, Rosa canina (Rosa canina) fruit oil, and the like. The oil and fat containing plant seed mainly include jojoba (Simmondsia chinensis) seed oil, safflower (Carthamus tinctorius) seed oil, white pond flower (Limnanthes alba) seed oil, sunflower (Helianthus annuus) seed oil, Macadamia ternifolia (Macadamia ternifolia) seed oil, Ricinus communis (Ricinus communis) seed oil, peony (Paeonia suffruticosa) seed oil, grape (Vitis vinifera) seed oil, grape fruit (Citrus paradisi) seed oil, apple (Pyrus malus) seed oil, pomegranate (Punica) seed oil, watermelon (Citrus lanatus) seed oil, pumpkin (Cucura peltata) seed oil, flax (Linum usitatissimum) seed oil, rape (Brassica) seed oil, and sesame (Sempnsaum indicum) seed oil.

Shea butter (shea butter) is a fruit directly extracted from an African shea tree, has soft, moist and smooth texture, is the most effective natural moisturizing product, and is widely applied to the field of cosmetics. For example, the document Chenpeofeng, the application of Shea butter in cosmetic varieties, Fujian light textile [ J ],2003,12(175):1-4, indicates that Shea butter can be used not only as an emollient in skin care emulsions and creams to achieve a powerful moisturizing effect, but also in products such as lipstick, lip gloss and sun protection, and has good adhesion, softness and non-greasiness, and can assist in increasing the SPF (sun protection factor). In addition, the edible oil shea butter can be obtained by fractionation of shea butter, such as: the research of extraction process of bioactive substances in shea butter in the Bodhisaea of Huangxian, Chinese oil J, 2015,40(4):69-73 proposes that shea butter can be used as a substitute of cocoa butter for making chocolate, candy and the like.

The olive oil is natural oil extracted from fresh olive fruits by a proper method, and is widely applied to the industries of medicine, daily chemicals and the like. For example, the literature is mazejora, the efficacy of natural vegetable oils in sunscreen cosmetics has been studied, and it is proposed in china oil [ J ],2021,46(1):71-75 that polyphenol components contained in olive oil have ultraviolet-absorbing and antioxidant effects, and also can eliminate facial wrinkles and prevent skin aging. The Wangcheng chapter, Chinese olive development process and industry prospect, and the biomass chemical engineering [ J ],2013,47(2):41-46 propose that olive oil can improve the digestive system, reduce cardiovascular diseases and prevent cancers, and the like, and meanwhile, oleic acid contained in olive oil can reduce blood pressure, balance blood fat and blood sugar. The olive oil has a slight skin feel, is very suitable for dry skin, contains squalene and fatty acid which can be quickly absorbed by the skin, effectively keeps the skin elastic and moist, contains abundant monounsaturated fatty acid, vitamin and phenolic substances, can eliminate facial wrinkles, prevents skin aging, and has the effects of protecting skin and hair, preventing rhagadia manus et pedis and the like.

Jojoba oil has a very remarkable beautifying effect on skin, can dredge pores, regulate oil secretion of oily or mixed skin, improve inflamed skin, eczema, psoriasis, pimple and the like, and can restore vitality and luster of dry hair and dry wrinkle skin, so that the jojoba oil is base oil which can be used for various skin properties. For example, in the document of lachrymal-elliottii, the application of jojoba oil in cosmetics, in daily chemical science [ J ],2007,30(2), it is pointed out that jojoba oil can significantly reduce the loss of epidermal water by incompletely blocking the evaporation of gas and water, and as an excellent moisturizing agent, it has the functions of easy spreading and lubrication, and makes the skin smooth and elastic.

Safflower oil contains 6% saturated fatty acid, 21% oleic acid and 73% linoleic acid. Because the main component of the linoleic acid is linoleic acid which is the highest linoleic acid in the food oil, the linoleic acid has high nutritive value and is concerned about medicines, foods, cosmetics and the like. For example, the research progress of safflower seed oil in Borel, World patent Medicine Information (Electronic Version [ J ],2021,21(27): 146-. The analysis of the efficacy and application prospect of the safflower seed oil in Jiangli and agricultural product processing [ J ],2017,6:56-58 indicates that the safflower seed oil can effectively prevent atherosclerosis, reduce the content of blood fat and serum cholesterol, soften blood vessels, indirectly recover a plurality of drug effect functions of a nervous system and the like after being eaten for a long time.

Meadowfoam seed oil is one of the most stable vegetable oils known, the high stability being due to the presence of the natural antioxidant tocopherol. For example, in Sutihan, the trace component analysis and the ultraviolet resistance and oxidation resistance research of several kinds of high oxidation stability vegetable oil are disclosed, the content and oxidation resistance of tocopherol in the meadowfoam seed oil are detected in the spice essence cosmetics [ J ],2019,4:47-51, and the removal of DPPH shows that the meadowfoam seed oil has certain oxidation resistance, can prevent ultraviolet rays, promotes the skin capillary circulation, and makes the face color naturally ruddy and active.

The rice bran oil is a vegetable oil with rich nutrition, and is rich in unsaturated fatty acid, octacosanol, gamma-oryzanol, phytosterol, tocotrienol, squalene and other functional substances. The rice bran oil contains 40-50% of oleic acid, 29-42% of linoleic acid, 1% of linolenic acid and 12-20% of palmitic acid. The rice bran oil is deeply processed, is widely applied to cosmetics, and is applied to cosmetics such as washing products, sun-screening and anti-aging products, skin disease treatment and the like. For example, Zhansenwang, stabilization of rice bran and its application in cosmetics, Jiangxi science [ J ],2007,25(1): 103-.

The sunflower seed oil contains more than 40 percent of unsaturated fatty acid, wherein the content of oleic acid is about 38 percent, the content of linoleic acid is about 55 percent, the content of linoleic acid is far higher than that of peanut oil and sesame oil, the sunflower seed oil is easy to be absorbed by human bodies, and the sunflower seed oil is internationally regarded as high-quality oil. For example, Zhaofurong, sunflower seed oil preparation and comprehensive utilization, Chinese oil J, 2005,30(1):9-13, proposed that the ratio of vitamin E in sunflower seed oil to unsaturated fatty acid is relatively balanced, which is beneficial for lipid peroxidation protection when the human body takes in unsaturated fatty acid, can also effectively reduce serum cholesterol level, and has the function of lowering blood pressure, thus being a good raw material for products such as cooking, margarine, shortening, mayonnaise and salad oil.

The skin barrier, generally referred to as the physical barrier of the skin, includes the stratum corneum layer of the epidermis of the skin and the sebaceous membrane located on the surface of the stratum corneum layer. The stratum corneum is located at the outermost layer of the epidermis and has a "brick-wall structure", wherein "bricks" refer to keratinocytes and "mortar" refers to intercellular lipids. In the process of skin barrier formation, more than 200 genes are highly associated with the skin barrier. For example, the barrier factors involved in the formation of the cornified envelope, silk polyprotein (FLG), Loricrin (LOR), endothelin (IVL), transglutaminase 1(TGM1), and the barrier moisturizing-related factors caspase 14(CASP14), HMGCoA reductase (HMGCR), aquaporin 3(AQP3), and the keratinocyte intercellular junction-related factor zonulin 1 (ZO-1).

In particular, Filaggrin (FLG) is an important constituent protein of the cornified envelope of the outer layer of the epidermis of the skin, mainly present in the stratum granulosum and stratum lucidum, and an important constituent structure of the skin barrier; and can be further degraded into hydrophilic free amino acids and water-absorbing derivatives, which can help skin store water and maintain skin water content, and has moisturizing effect. Loricrin (LOR) is a major component of the cornified envelope and plays an important role in the barrier function of the epidermis. Endothelin (IVL) is a marker protein for keratinocyte differentiation, mainly expressed in the upper part of the acanthocyte layer and the granular layer, and is located in the outer layer of the cornified envelope, and is covalently bound to a ceramide containing a hydroxyl group — OH, thereby linking the lipid matrix and the keratinocytes to each other to function. The characteristic resistance and insolubility of the cornified envelope is based on the very stable isopeptide bond formation catalyzed by transglutaminase 1(TGM 1).

HMGCoA reductase (HMGCR) is a rate-limiting enzyme for synthesizing cholesterol, exists in endoplasmic reticulum, catalyzes and synthesizes methyl dihydroxy pentanoic acid, then generates squalene through multi-step reactions such as decarboxylation, phosphorylation and the like, and finally converts the squalene into cholesterol after cyclization. Aquaporin 3(AQP3) is the most abundant aquaporin subtype expressed by AQPs family, has spatial hierarchy in the expression quantity of epidermis, and is mainly expressed in the basal layer, the acanthocyte layer and the granular layer of human epidermis and gradually disappears to the stratum corneum. This spatial distribution is consistent with the moisture content distribution of the skin: the water content of the basal layer and the upper part of the basal layer is about 75%, while the stratum corneum is only about 10% -15%. The high expression of AQP3 in the basal lamina membranacea can promote the transportation of water, glycerol and urea, so that the extracellular environment of the basal lamina membranacea is closer to a neutral equilibrium state; the closer to the stratum corneum, the less AQP3 was expressed, the more the water content decreased significantly, and the environment in the skin became increasingly acidic. In addition, AQP3 plays an important role in the transport of keratin glycerol, and AQP3 transports endogenous glycerol and triglycerides in sebaceous glands into the epidermis, so that it directly or indirectly affects the skin moisturizing effect.

Tight junctions are an important component of the physical barrier, mostly present in the epidermal layer of the skin, laterally at the top of the adjacent intercellular spaces. Tight junctions are composed of different types of transmembrane and intracellular cytoplasmic proteins. Transmembrane proteins such as sluice protein 1(CLDN1), Occludin (OCLN), etc.; cytoplasmic proteins include mainly zonula occludens-1 (ZO-1). ZO-1 is a cytoplasmic protein belonging to the family of membrane-associated guanylate kinase proteins, which aggregates atresia and sluice proteins via extracellular signaling pathways to form a basic cell junction band; ZO-1 is a cytoplasmic protein most characteristic of skin barrier function.

Plant biomimetic lipid technology (Phyto Bionic Sebum, PBS technology for short): a plurality of natural vegetable oils are selected, natural lipid components in a skin barrier structure are simulated through scientific proportioning, unsaturated fatty acids such as linolenic acid, linoleic acid and the like which are necessary for a skin cuticle are supplemented, lipid components lacking in the skin are supplemented, and the lipid components are compatible with sebum, so that the skin barrier is repaired quickly, and the skin is enabled to be in a healthy, moist and glossy state.

Wherein: the vegetable oil and fat is mainly derived from, but not limited to, plant fruits (e.g., shea butter, olive oil, almond oil, avocado oil, sea buckthorn oil, coconut oil, orange oil, bergamot oil, citron oil, tomato oil, rosa canina oil, piper oil, brazil nut oil, vanilla oil, anise oil, ruined palm oil, juniper berry oil, wenzhou mandarin fruit oil, star palm fruit oil, wild rose fruit oil, and coriander fruit oil), seeds (e.g., jojoba seed oil, safflower seed oil, meadowfoam nut oil, sunflower seed oil, macadamia, castor bean seed oil, peony seed oil, grape seed oil, apple seed oil, pomegranate seed oil, pumpkin seed oil, linseed oil, rapeseed oil, sesame seed oil, glass seed oil, brazil nut oil, berba seed oil, etc.), sesame seed oil, olive seed oil, apricot seed oil, olive seed oil, avocado seed oil, olive, Kapok seed oil, tea seed oil, Sichuan valley seed oil, papaya seed oil, raspberry seed oil, Adonis amurensis seed oil, calendula seed oil, bitter tree seed oil, moringa oleifera seed oil, pecan seed oil, perilla seed oil, babassu seed oil, white lupin seed oil, and the like) and germ (for example: rice germ oil, wheat germ oil, oat germ oil, corn germ oil, etc.), and also other parts such as leaves, barks, roots, petals, pistils, etc., which are partially derived from plants.

The invention unexpectedly discovers that the plant fruit oil (such as shea butter, olive oil and the like) and the plant seed oil (such as jojoba oil, safflower seed oil, sunflower seed oil and the like) have skin barrier related efficacy, can improve the skin barrier activity and promote the expression of skin barrier related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1. Therefore, the composition comprising the vegetable fruit oil and the vegetable seed oil can be applied to medicines and skin external preparations as a efficacy additive.

Disclosure of Invention

In one aspect, there is provided a composition comprising a vegetable fat comprising a vegetable fruit oil and a vegetable seed oil, wherein the vegetable fruit oil is selected from the group consisting of: shea butter, olive fruit oil, sweet almond oil, avocado fruit oil, sea buckthorn fruit oil, coconut fruit oil, orange fruit oil, bergamot fruit oil, citron fruit oil, tomato fruit oil, rosa canina fruit oil, pepper fruit oil, myrtle fruit oil, vanilla planifolia fruit oil, anise fruit oil, maoregano fruit oil, juniper berry oil, satsuma mandarin fruit oil, star palm fruit oil, dog rose fruit oil, coriander fruit oil, or combinations thereof; wherein the plant seed oil is selected from: jojoba seed oil, safflower seed oil, meadowfoam seed oil, sunflower seed oil, rice bran oil, macadamia nut seed oil, castor bean oil, peony seed oil, grape seed oil, grapefruit seed oil, apple seed oil, pomegranate seed oil, watermelon seed oil, pumpkin seed oil, linseed oil, rapeseed oil, sesame seed oil, glass chicory seed oil, burkholderia walnut seed oil, grass cotton seed oil, tea seed oil, Sichuan valley seed oil, papaya seed oil, raspberry seed oil, monkey-bushy seed oil, calendula seed oil, bitter tree seed oil, moringa seed oil, pecan seed oil, perilla seed oil, babassu seed oil, white lupin seed oil, or combinations thereof, wherein the composition comprises at least 0.5% by weight of a vegetable oil based on the total weight of the composition. In a preferred embodiment, the composition comprises a plant fruit oil and a plant seed oil in a weight ratio of plant fruit oil to plant seed oil of from 2:8 to 8: 2.

In a preferred embodiment, the composition of the invention further comprises a natural surfactant selected from the group consisting of: lecithin, cholesterol, lanolin, tea saponin, protein, saponins, saccharides, alkyl polyglycoside or their combination. In a preferred embodiment, the natural surfactant is hydrogenated lecithin.

In a preferred embodiment, the weight ratio of the vegetable oil or fat to the natural surfactant in the composition is from 1:1 to 20: 1. In a more preferred embodiment, the weight ratio of vegetable oil to natural surfactant in the composition is from 10:1 to 20: 1.

In another aspect, the present invention also relates to the use of a composition comprising vegetable oils for enhancing the barrier function of the skin. In a preferred embodiment, the enhancement of the skin barrier function is achieved by increasing the skin barrier activity and/or promoting the expression of skin barrier-associated factors.

In still another aspect, the present invention relates to the use of a composition comprising vegetable oils and fats for the preparation of a medicament and/or a skin external preparation for enhancing the barrier function of the skin. In a preferred embodiment, the pharmaceutical product and/or the external preparation for skin comprises 0.001 to 100% by weight of the composition.

Detailed Description

The present invention relates to a composition comprising vegetable fruit oil and vegetable seed oil and use thereof, which has been found in research to have effects of increasing skin barrier activity and promoting expression of skin barrier-related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1. Therefore, the composition comprising the plant fruit oil and the plant seed oil can be used as an efficacy additive for preparing medicines and/or skin external preparations for enhancing the skin barrier function.

To provide a more concise description, some of the quantitative representations presented herein are not modified by the term "about". It is understood that each quantity given herein is intended to refer to the actual given value, regardless of whether the term "about" is explicitly used, and also to refer to the approximation to such given value that would reasonably be inferred by one of ordinary skill in the art, including approximations due to experimental and/or measurement conditions for such given value.

To provide a more concise description, some quantitative expressions are recited herein as a range from about an X amount to about a Y amount. It should be understood that when a range is recited, the range is not limited to the upper and lower limits recited, but includes the entire range from about the X amount to about the Y amount or any amount therebetween.

Plant fruit oil

The plant fruit oil in the invention is also called plant fruit oil, which is mainly from plant fruits, seeds and embryos, and also has parts from other parts of plants such as leaves, barks, roots, petals, stamens and the like. Vegetable fruit oils include, but are not limited to, Butyrospermum parkii (Butyrospermum parkii) fruit fat (also known as shea butter), Olea europaea (Olea europaea) fruit oil (also known as olive oil), apricot (Prunus armeniaca) kernel oil (also known as sweet almond oil), avocado (Persea gratisma) fruit oil, Hippophae rhamnoides (Hippophae rhamnoides) fruit oil, Cocos nucifera fruit oil, Citrus junos (Citrus junos) fruit oil, Citrus bergamia (Citrus aurantium bergamia) fruit oil, Citrus medica (Citrus bergamia) fruit oil, Lycopersicon esculentum (Solomonisco sium) fruit oil, Citrus canina (Rosa canina) fruit oil, Pepper (Pipernium) fruit oil, Myrothecium brazianum (Carriera) fruit oil, Citrus depressa (Pimpinella) fruit oil, Citrus depressa officinalis fruit oil, Citrus depressa (Pimpinella) fruit oil, Citrus depressa (Pimpinella) fruit oil, and Pimpinella oil, Fruit oil of Rosa multiflora (Rosa multiflora) and fruit oil of Coriandrum sativum (Coriandrum sativum).

For example, shea butter has indexes close to those of sebum secretion oil of human body, contains rich non-saponified components, is easy to be absorbed by human body, can prevent drying and cracking, further recovers and maintains the natural elasticity of skin, protects the barrier function of skin, and also has the function of diminishing inflammation. The olive oil has a slight skin feel, is very suitable for dry skin, contains squalene and fatty acid which can be quickly absorbed by the skin, effectively keeps the skin elastic and moist, contains abundant monounsaturated fatty acid, vitamin and phenolic substances, can eliminate facial wrinkles, prevents skin aging, and has the effects of protecting skin and hair, preventing rhagadia manus et pedis and the like. The sweet almond oil can soften and condition skin, has good repairing and moisturizing effects on dry, cracked, itchy or irritated skin, and is particularly suitable for dry and sensitive skin. Avocado oil is used as a cosmetic lubricant, and has effects in smoothing skin, promoting skin to be smooth, promoting skin cell regeneration, and repairing sunburn and dry skin. Coconut oil is saturated fat, has stable lipid, is not easy to oxidize to generate free radical attack, has strong antioxidation capability to help human body to prevent the generation of free radicals, can be used for beautifying and protecting skin, has emulsification stabilizing effect and antioxidation, and can make cosmetics more uniform and fine.

The present invention has unexpectedly found that a composition comprising a plant fruit oil (e.g., shea butter, olive oil, etc.) has skin barrier-related efficacy, can increase skin barrier activity and promote the expression of skin barrier-related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1.

In some embodiments, the compositions of the present invention comprise from 0.1 to 100% by weight of vegetable fruit oil. In a preferred embodiment, the composition of the invention comprises from 0.1 to 50% by weight of vegetable fruit oil. In a preferred embodiment, the composition of the invention comprises from 0.1 to 20% by weight of vegetable fruit oil. In a preferred embodiment, the composition of the invention comprises from 0.5 to 10% by weight of vegetable fruit oil.

Plant seed oil

The plant seed oil used in the present invention is also called plant seed oil, including but not limited to: jojoba seed oil, safflower seed oil, meadowfoam seed oil and sunflower seed oil. The oil and fat containing plant seed mainly includes jojoba (Simmondsia chinensis) seed oil, safflower (Carthamus tinctorius) seed oil, Potentilla chinensis (Limnanthes alba) seed oil, sunflower (Helianthus annuus) seed oil, Macadamia ternifolia (Macadamia ternifolia) seed oil, Ricinus communis (Ricinus communis) seed oil, peony (Paeonia suffruticosa) seed oil, grape (Vitisia vinifera) seed oil, grape fruit (Citrus paradisi) seed oil, apple (Pyrus malus) seed oil, pomegranate (Punica grantum) seed oil, watermelon (Citrullus lanatus) seed oil, pumpkin (Cucura peltata) seed oil, flax (Linum usitatissimum) seed oil, rape (Brassica campestris) seed oil, sesame (Sempm sambucinum) seed oil, glass papaya (Borax grandis) seed oil, Caryopteria mangium sativum seed oil, papaya seed oil (Acacia mangium seed oil), Raphus sativum seed oil, Raphus chinensis seed oil, Rubus sativus seed oil, Raphus sativus seed oil, and Caryopteri seed oil, Monkey tree (Adansonia digita) seed oil, Calendula officinalis (Calendala officinalis) seed oil, bitter tree (Carapa guaianensis) seed oil, Moringa oleifera (Moringa oleifera) seed oil, Carya illinoinensis (Carya illinoinensis) seed oil, Perilla frutescens (Perilla octoides) seed oil, Babesia babassu (Orbignya oleica) seed oil, Lupinus albus (Lupinus albus) seed oil, and the like.

For example, jojoba seed oil is the most permeable base oil, is easily absorbed by skin, is fresh, moist and non-greasy, can restore skin pH balance, remove wrinkles, effectively improve oily skin, condition sebaceous gland secretion function, shrink pores, and is also the best skin moisturizing oil. The safflower seed oil is rich in linoleic acid and fat accompanying substances (vitamin E and vitamin A), is easy to deteriorate when contacting with air, has similar nutritional ingredients to sunflower oil, is added into cosmetics for generally moistening and lubricating skin, can improve eczema and rough skin, enables the skin to become tender and smooth, and helps to normalize metabolism. The meadowfoam seed oil contains more than 98% of long-chain fatty acid with an anti-oxidation effect, has a unique molecular structure, has a moisturizing function, can form a water-locking film on the surface of skin, locks water to resist dryness, can stretch skin texture, deeply repairs skin loss and improves the overall skin contour. The macadimia nut seed oil is characterized by being rich in unsaturated fatty acid, can prevent heart disease, can regulate and control blood sugar level, improves lipid metabolism of diabetics, can effectively moisturize and protect skin, and is a good sun-proof and skin-care product.

The present invention has surprisingly found that compositions comprising plant seed oil (e.g., jojoba oil, safflower seed oil, sunflower seed oil, etc.) have skin barrier related efficacy, can increase skin barrier activity and promote expression of skin barrier related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3, and ZO-1.

In some embodiments, the compositions of the present invention comprise 0.1 to 100% by weight of plant seed oil. In a preferred embodiment, the composition of the invention comprises 0.1 to 50 wt% of plant seed oil. In a preferred embodiment, the composition of the present invention comprises 0.1 to 20 wt% of plant seed oil. In a preferred embodiment, the composition of the invention comprises 0.5-10 wt% of plant seed oil.

Composition comprising vegetable fruit oil and vegetable seed oil

In some embodiments of the present invention, there is provided a composition comprising a plant fruit oil and a plant seed oil, wherein the weight ratio of the plant fruit oil to the plant seed oil is from 2:8 to 8: 2. In a preferred embodiment, the weight ratio of the plant fruit oil to the plant seed oil is 1: 1.

In some embodiments, the compositions of the present invention comprise from 0.1 to 100% by weight of vegetable fruit oil and vegetable seed oil. In a preferred embodiment, the composition of the invention comprises from 0.1 to 50% by weight of vegetable fruit oil and vegetable seed oil. In a preferred embodiment, the composition of the invention comprises 0.1 to 20 wt% of vegetable fruit oil and vegetable seed oil. In a preferred embodiment, the composition of the invention comprises 0.5 to 10% by weight of vegetable fruit oil and vegetable seed oil.

Natural surfactant

The compositions of the present invention may also comprise natural surfactants. Natural surfactants are mostly derived from animal and plant bodies and are relatively complex high molecular organic substances. Because of its strong hydrophilicity, it can form an emulsion. These substances usually have a high viscosity, which is beneficial to emulsion stability.

Natural surfactants included in the compositions of the present invention include, but are not limited to: lecithin, cholesterol, lanolin, tea saponin, protein, saponins, saccharides, alkyl polyglycoside, etc. Lecithin exists in biological cells, such as animal egg, brain, etc., and plant seed or embryo, and yolk phospholipid is extracted from yolk; soybean is rich in lecithin. Lecithin has physiological activities of emulsification, dispersion, oxidation resistance and the like, and is a natural excellent surfactant and an important emulsifier.

The hydrogenated lecithin has strong hydrophilicity and moisture retention, has strong affinity to skin and mucous membrane, and can play the roles of moisture retention, emulsification, dispersion and the like when being used in a formula of cosmetics; as a surfactant, the oil-water-free emulsion can also condition the skin to achieve a good oil-water balance effect, and the hydrogenated lecithin can be used for developing advanced cosmetic products such as skin care cream, hand cream, lipstick, sunscreen oil and the like.

In some embodiments, the compositions of the present invention comprise from 0.1 to 20 wt% of a natural surfactant. In a preferred embodiment, the composition of the invention comprises from 0.1 to 10% by weight of natural surfactant. In a preferred embodiment, the composition of the invention comprises 0.1 to 5 wt% of a natural surfactant. In a preferred embodiment, the composition of the invention comprises 0.5 to 2 wt% of a natural surfactant.

In a preferred embodiment, the weight ratio of vegetable fats and oils (including vegetable fruit oils and vegetable seed oils) to natural surfactants in the compositions of the present invention is from 1:1 to 20: 1. In a more preferred embodiment, the weight ratio of vegetable oil or fat (including vegetable fruit oil and vegetable seed oil) to natural surfactant in the composition is from 10:1 to 20: 1.

Drug and/or external preparation for skin

The composition comprising the plant seed oil can be applied to medicines and/or skin external preparations for enhancing the skin barrier function.

In some embodiments, the pharmaceutical product is selected from: tablets, capsules, emulsions, suspensions, powders, granules, solutions, and various pharmaceutical dosage forms known in the art. Different amounts are added according to different types of dosage forms.

In some embodiments, the external skin agent is selected from: face cleaning lotion, cosmetic water, lotion, cream, jelly and facial mask. Different amounts are added according to different types of preparations.

The external preparation for skin is a general concept of all ingredients generally used for the external part of skin, and may be, for example, a cosmetic composition. The cosmetic composition can be basic cosmetics, face makeup cosmetics, body makeup cosmetics, hair care cosmetics and the like, and the dosage form of the cosmetic composition is not particularly limited and can be reasonably selected according to different purposes. The cosmetic composition also contains different cosmetically acceptable media or matrix excipients according to different dosage forms and purposes.

The external preparation for skin contains a dermatologically acceptable carrier or vehicle (e.g., lotion, cream, ointment, cleanser, etc.). One of ordinary skill in the art will be able to select a carrier that will dissolve or disperse the components in the concentrations described above, based on common general knowledge in the art.

One of ordinary skill in the art will be able to select suitable carriers, including for example water, alcohols, oils, and the like, based on the common general knowledge and their ability to dissolve or disperse in the active ingredient at the concentrations most suitable for treatment.

The skin external preparation of the present invention may be in the form of a topical application product, which can be externally applied to the skin, and can be prepared by those ordinary techniques well known in the art. The carrier may take any of a variety of practical forms such as a cream, dressing, gel, lotion, ointment or liquid comprising the applied and rinsed-off composition and incorporated into a carrier of material such as a dry or wet spread, hydrogel matrix, or adhesive (or non-adhesive) patch by methods well known in the art. Preferably, the carrier is a gel or a lotion that adds moisture, or a spread in dry or wet form.

Typical carriers include emulsions comprising water and/or an alcohol and an emollient such as hydrocarbon oils and waxes, silicone oils, hyaluronic acid, vegetable, animal or marine fats or oils, glyceride derivatives, fatty acids, or fatty acid esters or alcohols or alcohol ethers, lanolin and its derivatives, polyols or esters, wax esters, sterols, phospholipids and the like, and typically emulsifiers (nonionic, cationic or anionic), although some emollients inherently have emulsifying properties. In addition, these same components may be formulated into creams, gels, or solid sticks using different ratios of their components and/or by incorporating thickeners such as gums or other forms of hydrocolloids.

The skin external agent of the present invention may contain additional components commonly found in skin care compositions, such as emollients, skin conditioning agents, emulsifiers, preservatives, antioxidants, fragrances, chelating agents, and the like, as long as they are physically and chemically compatible with the other components of the skin external agent and do not affect the effect of the plant seed oil of the present invention.

In some embodiments of the external preparation for skin of the present invention, one or more preservatives may be used. Suitable preservatives include p-hydroxyacetophenone, C1-C4 alkyl parabens, and phenoxyethanol. Preservatives are used in amounts of about 0.5 to about 2 weight percent, preferably about 0.5 to 1 weight percent, based on the total weight of the composition.

In one example of the external preparation for skin of the present invention, one or more antioxidants may be used. Suitable antioxidants include Butylated Hydroxytoluene (BHT), ascorbyl palmitate (BHA), butylated hydroxyanisole, phenyl-alpha-naphthylamine, hydroquinone, gallopropyl, nordihydroguaiaretic acid, vitamin E or derivatives of vitamin E, vitamin C and its derivatives, calcium pantothenate, green tea extract and mixed polyphenols, and mixtures of the above. The antioxidant is used in an amount ranging from about 0.02 to 0.5 weight percent, more preferably from about 0.002 to 0.1 weight percent, based on the total weight of the composition.

In one example of the external preparation for skin of the present invention, one or more emollients may be used, functioning as a lubricant by their ability to remain on the surface of the skin or in the stratum corneum, to reduce flaking, and to improve the appearance of the skin. Typical emollients include fatty esters, fatty alcohols, mineral oils, polyether siloxane copolymers, and the like. Examples of suitable emollients include, without limitation, polypropylene glycol ("PPG") -15 stearyl ether, PPG-10 cetyl ether, Steareth-10, Oleth-8, PPG-4 lauryl ether, vitamin E acetate, lanolin, cetyl alcohol, cetearyl ethylhexanoate, cetearyl alcohol, glyceryl stearate, octyl hydroxystearate, dimethylpolysiloxane, and combinations thereof. Cetyl alcohol, cetearyl ethylhexanoate, cetostearyl alcohol, glyceryl stearate and combinations thereof are preferred. When used, the emollient is present in an amount ranging from about 0.1 to about 30 weight percent, preferably from about 1 to about 30 weight percent, based on the total weight of the composition.

In one example of the external preparation for skin of the present invention, one or more moisturizers may also be used. Humectants, also known as humectants, help to increase the effectiveness of the emollient, reduce flaking, stimulate the removal of compositional scales, and improve skin feel. Polyhydric alcohols may be used as humectants, including, but not limited to, glycerin, polyalkylene glycols, alkylene polyols and derivatives thereof, including butylene glycol, propylene glycol, dipropylene glycol, polyglycerol, polyethylene glycol and derivatives thereof, sorbitol, hydroxypropyl sorbitol, hexylene glycol, 1, 3-dibutylene glycol, 1,2, 6-hexanetriol, ethoxylated glycerin, propoxylated glycerin, and combinations thereof. When used, the humectant is present in an amount of about 0.1 to about 20 weight percent, preferably about 1 to about 15 weight percent, based on the total weight of the composition.

In one example of the external preparation for skin of the present invention, one or more emulsifiers may be used. The emulsifier may be used in an effective stabilizing amount range. Preferably, the emulsifier is used in an amount of about 1.0 to about 10.0 wt%, more preferably about 3.0 to about 6.0 wt%, based on the total weight of the composition. Any emulsifier that is compatible with the components of the composition may be used. Suitable emulsifiers include stearic acid, cetyl alcohol, glyceryl stearate, lecithin, stearyl alcohol, Steareth-2, Steareth-20, acrylic/C10-30 alkanol acrylate crosspolymer, and combinations thereof.

In one example of the external preparation for skin of the present invention, one or more pH adjusting agents may be used. The pH adjusting agent useful in the skin external preparation of the present invention includes tromethamine. When used, the pH adjusting agent is used in an amount of about 0.1 to about 2 weight percent, preferably about 0.1 to about 1 weight percent, based on the total weight of the composition.

In one embodiment of the present invention, the external preparation for skin comprises acrylic acid/C10-30 alkanol acrylate crosspolymer, glycerin, p-hydroxyacetophenone, glyceryl stearate and lecithin, cetostearyl alcohol, cetearyl ethyl hexanoate, tromethamine or a combination thereof.

In some embodiments of the present invention, the composition of the present invention is used in an amount of 0.001% to 100% (w/w), preferably 0.01% to 60% (w/w), and more preferably 0.01% to 40% (w/w) in the skin external preparation.

Examples

The invention will be further illustrated by the following specific examples. It should be noted that the examples are given solely for the purpose of illustration and are not to be construed as limitations on the scope of the invention, as many insubstantial modifications and variations of the invention may be made by those skilled in the art in light of the above teachings. Test methods in which specific conditions are not specified in the following examples are generally carried out under conventional conditions or under conditions recommended by the manufacturer. All percentages and parts are by weight unless otherwise indicated.

The experimental materials used in the following examples include:

butyrospermum PARKII (Butyrum PARKII) butter, a trade name of refined shea butter, available from basf New materials Co., Ltd

Olive (OLEA EUROPAEA) fruit oil, sold under the trade name refined olive oil, available from Textron tenicnica, s.l.u.

Jojoba seed oil, tradename jojoba oil, is available from Vantage Specialty Ingredients, Inc.

Safflower (CARTHAMUS TINCTORIUS) seed oil, commercially available as safflower seed oil, is available from Northstar Lipids UK Ltd.

Potentilla chinensis (LIMNANTHES ALBA) seed oil, tradename Potentilla chinensis seed oil, available from Haimines specialty Chemicals

Sunflower (Helianthus ANNUUS) seed oil, tradename sunflower seed oil, available from Jiaji grain oil (Nantong) Ltd

Hydrogenated lecithin, trade name hydrogenated lecithin, was purchased from NIKKO CHEMICALS co.

The experimental apparatus used in the examples included:

weighing a balance: PB4002-N type from METTLER TOLEDO

A pH meter: SEVENCULTI-TYPE METTLER TOLEDO CORPORATION

A water bath kettle: electric heating constant temperature water bath kettle of Shanghai-Hengshi Co Ltd HWS28

A homogenizer: POLYTRON PT 3100D

A desk type stirrer: IKA (IKA)^@EUROSTAR,power control-visc。

Example 1: preparation of the composition

Weighing 0.5 part by mass of hydrogenated lecithin in a beaker, adding 99.5 parts by mass of deionized water, and uniformly stirring for later use.

Example 2: preparation of a composition comprising vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 0.5 part by mass of shea butter in a beaker, heating to 75-80 ℃ while stirring, then weighing 99 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 3: preparation of a composition comprising vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 2.5 parts by mass of shea butter in a beaker, heating to 75-80 ℃ while stirring, then weighing 97 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 4: preparation of a composition comprising vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 5 parts by mass of shea butter in a beaker, heating to 75-80 ℃ while stirring, then weighing 94.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 5: preparation of a composition comprising vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 7.5 parts by mass of shea butter in a beaker, heating to 75-80 ℃ while stirring, then weighing 92 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 6: preparation of a composition comprising vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 10 parts by mass of shea butter in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 7: preparation of a composition comprising a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin and 0.5 part by mass of jojoba oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 99 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 8: preparation of a composition comprising a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin and 2.5 parts by mass of jojoba oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 97 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 9: preparation of a composition comprising a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin and 5 parts by mass of jojoba oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 94.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 10: preparation of a composition comprising a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin and 7.5 parts by mass of jojoba oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 92.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 11: preparation of a composition comprising a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin and 10 parts by mass of jojoba oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 12: preparation of a composition comprising a vegetable fruit oil and a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin, 5 parts by mass of shea butter and 5 parts by mass of jojoba oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 13: preparation of a composition comprising a vegetable fruit oil and a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin, 2 parts by mass of shea butter and 8 parts by mass of jojoba oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 14: preparation of a composition comprising a vegetable fruit oil and a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin, 4 parts by mass of shea butter and 6 parts by mass of jojoba oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 15: preparation of a composition comprising a vegetable fruit oil and a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin, 6 parts by mass of shea butter and 4 parts by mass of jojoba oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 16: preparation of a composition comprising a vegetable fruit oil and a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin, 8 parts by mass of shea butter and 2 parts by mass of jojoba oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 17: preparation of a composition comprising a vegetable fruit oil and a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin, 5 parts by mass of shea butter and 5 parts by mass of olive oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 18: preparation of a composition comprising a vegetable fruit oil and a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin, 5 parts by mass of shea butter and 5 parts by mass of safflower seed oil, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in a beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 19: preparation of a composition comprising a vegetable fruit oil and a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin, 5 parts by mass of shea butter and 5 parts by mass of white pond flower seed oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 20: preparation of a composition comprising a vegetable fruit oil and a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin, 5 parts by mass of shea butter and 5 parts by mass of rice bran oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 minutes for later use.

Example 21: preparation of a composition comprising a vegetable fruit oil and a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin, 5 parts by mass of shea butter and 5 parts by mass of sunflower seed oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 minutes for later use.

Example 22: preparation of a composition comprising a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin and 5 parts by mass of safflower seed oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 94.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 23: preparation of a composition comprising vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 5 parts by mass of olive oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 94.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 24: preparation of a composition comprising a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin and 5 parts by mass of sunflower seed oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 94.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 25: preparation of a composition comprising a vegetable seed oil

Weighing 0.5 part by mass of hydrogenated lecithin and 5 parts by mass of white Potentilla chinensis seed oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 94.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 26: preparation of the composition

Weighing 100 parts by mass of shea butter and testing.

Example 27: preparation of the composition

Weighing 100 parts by mass of jojoba oil to be tested.

Example 28: preparation of the composition

Weighing 100 parts by mass of safflower seed oil to be tested.

Test example 1: 3D epidermal recombination model tissue viability test

The 3D epidermal recombination model is a human 3D epidermal model and can simulate the structure of each layer of the epidermis differentiated by keratinocytes. According to the 3D epidermal recombination model tissue viability testing method, firstly, a certain damage is caused to the model by using a skin irritant SDS, and then, a proper amount of sample prepared by the embodiment is covered on the surface of the damaged model for 24 hours to repair. After 24 hours, the samples were washed away and tested for tissue viability recovery using the MTT method. The skin barrier repair ability of the example samples can be evaluated according to the tissue viability recovery.

1. Test materials

1.1 cells: the skin model is a 3D epidermal skin model

(EpiSkin)。

1.2 reagent:

3D skin model maintenance medium, 3D skin model detection medium (EpiSkin); sodium Dodecyl Sulfate (SDS) (Sigma); calcium and magnesium containing dpbs (gibco); mtt (sigma); isopropanol and concentrated hydrochloric acid (national drug group).

1.3 Main Equipment

CO₂An incubator HERAcell 240i, a biological safety cabinet 1300SERIES A2, a microplate reader Multiskan Mk3 (Thermo); forward pipettor M25 (Gilson); 50mL Multipette plus (Eppendorf) continuous applicator tip; skin punch (area 0.38 cm)²)。

2. The experimental method comprises the following steps:

3D recombinant epidermal model reception and resuscitation

Receiving the 3D epidermal recombination model, checking the integrity of the package, checking the number of models, checking the maintenance medium, and detecting the color and volume of the medium. And after the error is confirmed, starting to perform model recovery.

The resuscitation operation is performed in a biosafety cabinet. A number of 12-well plates were taken, 2 ml/well of maintenance medium was added to the first row, then the 3D recombinant epidermis model was removed with forceps, the surface gel was carefully removed with a cotton swab, placed in the wells containing maintenance medium, and allowed to resuscitate for 24 hours. One 12-well plate can resuscitate 4 3D recombinant epidermal models. The culture conditions are as follows: 5% CO₂And culturing in a constant-temperature incubator at 37 ℃.

3D recombination epidermis model damage treatment and sample adding product

A1.5% (w/v) Sodium Dodecyl Sulfate (SDS) solution was prepared using a 1 XPhosphate buffer solution (DPBS).

The model was divided into a blank control group (3 replicates without any treatment), a negative control group (3 replicates with 1.5% SDS) and sample groups of examples 1-21 and 26-28 (3 replicates per sample).

2 ml/well of maintenance medium was added to the second row of the 12-well plate, and then the 3D recombinant epidermal model located in the first row was transferred to the corresponding second row with tweezers.

50 microliters of 1.5% SDS solution was added dropwise to the surface of the negative control and sample groups of examples 1-6 and 8-9 using a pipette, and the coverage was secured to be uniform, and incubated at room temperature for 15 minutes. After 15 minutes, immediately wash with 1 × DPBS and gently wipe the residual liquid with a cotton swab. Then, 15. mu.l of the sample was applied to the surface of the lesion-treated (1.5% SDS) sample group model by using a piston displacement pipette (M25) and treated for 24 hours. All models were placed in a cell incubator under the following culture conditions: 5% CO₂And culturing in a constant-temperature incubator at 37 ℃.

3D recombinant epidermal model viability test

3 mg/ml of thiazole blue (MTT) stock was prepared with 1 XDPBS and the MTT stock was diluted to 0.3 mg/ml in the test medium. 2 ml/well of diluted MTT solution was added in the third row of the 12-well plate.

The sample on the surface of the model was washed off (not directly aligned for washing, but aligned to the side wall of the skin model support frame) by pipetting 1 XDPBS with a 50ml continuous pipette, and then pipetting the residual liquid with a cotton swab into a 12-well plate in the third line MTT solution. Finally placing the mixture into a cell culture box, and placing the cell culture box at 37 ℃ and 5% CO₂Was incubated in the incubator of (1) for 3 hours.

The acidic isopropanol was prepared and 1.8 ml of hydrochloric acid was added to 500 ml of isopropanol. A corresponding number of 1.5 ml microcentrifuge tubes (EP tubes) were molded and 500. mu.l of the acidic isopropanol was added to each tube. After the epidermal tissue was incubated in MTT for 3 hours, the epidermal tissue was removed with a punch, separated from the underlying collagen matrix with two small-gauge elbow forceps, and inverted, and then transferred together with the collagen matrix to a 1.5 ml EP tube containing acidic isopropanol. The EP tube was shaken and mixed on a vortex, and placed in a refrigerator at 4 ℃ for 72 hours for color extraction. Meanwhile, prepare an EP tube containing 1 ml of acid isopropanol, as the blank hole when the enzyme-linked immunosorbent assay detects.

The 1.5 ml EP tube containing the acid isopropanol and the skin model was taken out from the 4 ℃ freezer, the solution was mixed by shaking, 150 μ l of the extraction solution was taken out per tube and transferred to a 96-well plate, and 3 times of the extraction solution were taken out and added to the 96-well plate. Meanwhile, 3-6 wells of acidic isopropanol were prepared as blank control during detection, excluding background optical density (OD value). The OD was read at a wavelength of 570 nm. Relative activity of each group of models (blank control 100%) was calculated according to the following formula:

3. result judgment

The blank control group is an undamaged group, and the relative activity is 100%; the relative activity of the negative control group is controlled to be 40-60% compared with that of the blank control group; a higher relative activity in the sample group than in the negative control group indicates that the sample group has the ability to promote barrier repair, and a lower relative activity in the sample group than in the negative control group indicates that the sample group does not have the ability to promote barrier repair.

Table 1: MTT method for detecting relative activity condition of each group of tissues

As shown in table 1, the average relative viability of the skin model tissue of the blank control group was 100%, and the average relative viability of the skin model tissue of the negative control group (1.5% SDS treatment) was 41.0%. Example 1 (0% + jojoba oil 0%) skin model tissue average relative viability was 48.4%, example 2 (0.5%) skin model tissue average relative viability was 64.1%, example 3 (2.5%) skin model tissue average relative viability was 75.5%, example 4 (5%) skin model tissue average relative viability was 75.3%, example 5 (7.5%) skin model tissue average relative viability was 76.1%, example 6 (10% shea butter) skin model tissue average relative viability was 76.1%, example 7 (0.5% jojoba oil) skin model tissue average relative viability was 60.6%, example 8 (2.5% jojoba oil) skin model tissue average relative viability was 57.0%, example 9 (5% jojoba oil) skin model tissue average relative viability was 66.9%, example 10 (jojoba oil 7.5%) skin model tissue average relative viability was 68.9%, example 11 (jojoba oil 10%) skin model tissue average relative viability was 63.0%, example 12 (shea butter 5% + jojoba oil 5%) skin model tissue average relative viability was 66.5%, example 13 (shea butter 2% + jojoba oil 8%) skin model tissue average relative viability was 76.9%, example 14 (shea butter 4% + jojoba oil 6%) skin model tissue average relative viability was 69.7%, example 15 (shea butter 6% + jojoba oil 4%) skin model tissue average relative viability was 69.8%, example 16 (shea butter 8% + jojoba oil 2%) skin model tissue average relative viability was 57.4%, example 17% (shea butter 5% + jojoba oil 5%) skin model tissue average relative viability was 69.0%, example 18 (shea butter 5% + safflower seed oil 5%) skin model tissue mean relative viability was 54.3%, example 19 (shea butter 5% + meadow seed oil 5%) skin model tissue mean relative viability was 68.0%, example 20 (shea butter 5% + rice bran oil 5%) skin model tissue mean relative viability was 52.5%, example 21 (shea butter 5% + sunflower seed oil 5%) skin model tissue mean relative viability was 54.2%, example 26 (shea butter 100%) skin model tissue mean relative viability was 68.4%, example 27 (jojoba oil 100%) skin model tissue mean relative viability was 65.2%, example 28 (safflower seed oil 100%) skin model tissue mean relative viability was 70.8% (see table 1).

In conclusion, the shea butter in examples 2-6 and 26 has a better tissue repair ability in the concentration range of 0.5-100%; jojoba oil in examples 7-11 and 27 has better tissue repair capacity in the concentration range of 0.5-100%; in examples 12-16, Shea butter and jojoba oil have good tissue repair ability within a compounding ratio of 5:5/2:8/4:6/6:4/8: 2; in examples 17-21, shea butter compounded with olive oil, safflower seed oil, rice bran oil, and sunflower seed oil (5% + 5%) has better tissue repair ability.

Test example 2: 3D recombinant skin epidermal model barrier-related gene expression test

The 3D epidermal recombination model is a human 3D epidermal model and can simulate the structure of each layer of the epidermis differentiated by keratinocytes. Covering a proper amount of samples on an epidermis model for 24 hours, then collecting total ribonucleic acid (RNA) of epidermis tissues, and quantitatively detecting related genes of the skin barrier by adopting an RNA reverse transcription technology and a gene microarray technology so as to evaluate the skin barrier moisturizing and repairing capacity of the samples.

1. Test materials

1.1 cells: the skin model is a 3D epidermal skin model

(EpiSkin)。

1.2 reagent:

3D skin model maintenance medium (EpiSkin); calcium and magnesium containing dpbs (gibco); isopropanol, 75% absolute ethanol (national pharmaceutical group); DEPC water (tiangen organisms); total RNA extraction reagent trizol (thermofisher); reverse transcription Kit iScript cDNA Synthesis Kit and fluorescent real-time quantitative PCR reagent iTaqTMUniversal

Green Supermix(Bio-rad)。

1.3 Main Equipment

CO₂Incubator HERACell 240i, biosafety cabinet 1300SERIES A2, ultramicro UV spectrophotometer NanoDrop ONE (Thermo); gene amplification instrument C1000 Touch, real-time quantitative PCR instrument CFX Connect (Bio-rad); high speed refrigerated centrifuge MIKRO 220r (hittich); forward pipettor M25 (Gilson); 50mL Multipette plus (Eppendorf) continuous applicator tip; skin punch (area 0.38 cm)²)。

2. The experimental method comprises the following steps:

3D recombinant epidermal model reception and resuscitation

3D recombinant epidermal model sample treatment

The model was divided into the sample groups of examples 1,4, 9, 12, 17-25 (3 replicates per sample) and the positive control group (rosiglitazone, 3 replicates).

A piston displacement pipettor (M25) was used to add 15 microliters of rosiglitazone solution (100 micromoles in 1 x DPBS) to the positive control group, 15 microliters of sample was added to the sample group, and the samples were gently spread evenly over the skin model surface using a suction nozzle, in triplicate for each sample. All models were placed in a cell incubator and incubated for 24 hours. The culture conditions are as follows: 5% CO₂And culturing in a constant-temperature incubator at 37 ℃.

3D recombinant epidermal model Collection of Total RNA samples

The 1 XDPBS was aspirated with a 50ml continuous applicator, the sample was rinsed off the surface of the model (the sample was not directly aligned for rinsing, but was aligned with the side wall of the skin model support frame), and then the residual liquid was aspirated with a cotton swab. The epidermal tissue was cut with a punch, separated from the underlying collagen base with two small-gauge bent tweezers, placed in an EP tube containing 1 ml of total RNA extraction reagent (TRIzol), and repeatedly tapped with 1 ml of a rnase-free pipette tip until only the transparent stratum corneum remained. Subsequently, 0.2 ml of chloroform was added to the original EP tube, vigorously shaken, and centrifuged at a high speed at a low temperature for 15 minutes. Taking 500 microliters of the upper aqueous phase by using a pipette nozzle without RNase, adding equal volume of precooled (minus 20 ℃) isopropanol, reversing, mixing uniformly, and centrifuging at a high speed for 10 minutes at a low temperature. The centrifuged RNA forms a white flaky precipitate at the bottom of the EP tube, the precipitate is rinsed twice with 75% ethanol, residual ethanol is removed by a centrifuge and a suction nozzle, and the precipitate is opened and dried to be transparent at room temperature. With 40. mu.l RNase-free H₂Dissolving O, and mixing. The RNA concentration was measured again using a microspectrophotometer Nanodrop and the integrity of the RNA was checked by agarose gel electrophoresis. The dissolved total RNA of the epidermal model tissue is used for subsequent experiments or is stored for a long time at minus 80 ℃ after being subpackaged.

Quantitative detection of skin barrier related gene by RT-qPCR method

Taking 1 microgram of total RNA for reverse transcription, and adopting a reverse transcription Kit iScript cDNA Synthesis Kit for reverse transcription to obtain cDNA.

The reverse transcription system is as follows:

the reverse transcription procedure was as follows: 5 minutes at 25 ℃; 30 minutes at 42 ℃; 5 minutes at 85 ℃.

Real-time quantification of Polymerase Chain Reaction (PCR) reagent iTaqTMUniversal with fluorescence

Green SuPermix carries out fluorescence real-time quantitative detection and analyzes the expression condition of messenger RNA (mRNA) of the barrier related gene.

The reaction system is as follows:

the reaction procedure was as follows: at 95 ℃ for 30 seconds; at 95 deg.C, 15 seconds, 60 deg.C, 30 seconds, 40 cycles; 65-95 deg.C, each time by 0.5 deg.C (melting curve).

The primer sequences used in this example were designed as follows:

by using 2^-△△CtThe gene expression of the sample group and the positive control group (rosiglitazone) of example was analyzed for changes relative to the gene expression of the control group of example 1.

3. Result judgment

Example 1 the gene expression levels of the sample groups were taken as 100%, and each group compared to it, more than 100%, indicated up-regulation of gene expression, and less than 100%, indicated down-regulation of gene expression. FLG, LOR and other genes are up-regulated, which indicates that the sample group has the capacity of moisturizing and promoting barrier repair; genes such as FLG, LOR were down-regulated, indicating that this sample group did not have the ability to moisturize and promote barrier repair.

Table 2: skin barrier factor expression after treatment of each group of samples

As shown in table 2, the relative gene expression level of example 1 (0% of shea butter + 0% of jojoba oil) as a control is 100%, and the gene expression levels of the samples of examples 4, 9, 12, 17, 18, 19, 20, 21, 22, 23, 24 and 25 and the positive control sample are compared to obtain the corresponding percentage.

Specifically, the barrier factor FLG was compared to the samples of example 1 (shea butter 0% + jojoba oil 0%) after the treatment of the samples of example 4 (shea butter 5%), the samples of example 9 (jojoba oil 5%), the samples of example 12 (shea butter 5% + jojoba oil 5%), the samples of example 17 (shea butter 5% + olive oil 5%), the samples of example 18 (shea butter 5% + safflower seed oil 5%), the samples of example 19 (shea butter 5% + meadow seed oil 5%), the samples of example 20 (shea butter 5% + rice bran oil 5%), the samples of example 21 (shea butter 5% + sunflower seed oil 5%), the samples of example 22 (safflower seed oil 5%), the samples of example 23 (olive oil 5%), the samples of example 24 (sunflower seed oil 5%), the samples of example 25 (meadow seed oil 5%) and the positive control (rosiglitazone), 222%, 218%, 225%, 265%, 283%, 223%, 258%, 213%, 274%, 205%, 283%, 175% and 362%, respectively.

Specifically, the barrier factor LOR was compared to the samples of example 1 (shea butter 0% + jojoba oil 0%) after the treatment of the samples of example 4 (shea butter 5%), the samples of example 9 (jojoba oil 5%), the samples of example 12 (shea butter 5% + jojoba oil 5%), the samples of example 17 (shea butter 5% + olive oil 5%), the samples of example 18 (shea butter 5% + safflower seed oil 5%), the samples of example 19 (shea butter 5% + meadow seed oil 5%), the samples of example 20 (shea butter 5% + rice bran oil 5%), the samples of example 21 (shea butter 5% + sunflower seed oil 5%), the samples of example 22 (safflower seed oil 5%), the samples of example 23 (olive oil 5%), the samples of example 24 (sunflower seed oil 5%), the samples of example 25 (meadow seed oil 5%) and the positive control (rosiglitazone), 240%, 236%, 227%, 242%, 247%, 208%, 281%, 217%, 307%, 216%, 293%, 201% and 351%, respectively.

Specifically, the barrier factor IVL was compared to the samples of example 1 (shea butter 0% + jojoba oil 0%) after the treatment of the samples of example 4 (shea butter 5%), the samples of example 9 (jojoba oil 5%), the samples of example 12 (shea butter 5% + jojoba oil 5%), the samples of example 17 (shea butter 5% + olive oil 5%), the samples of example 18 (shea butter 5% + safflower seed oil 5%), the samples of example 19 (shea butter 5% + meadow seed oil 5%), the samples of example 20 (shea butter 5% + rice bran oil 5%), the samples of example 21 (shea butter 5% + sunflower seed oil 5%), the samples of example 22 (safflower seed oil 5%), the samples of example 23 (olive oil 5%), the samples of example 24 (sunflower seed oil 5%), the samples of example 25 (meadow seed oil 5%) and the positive control (rosiglitazone), 113%, 87%, 130%, 107%, 109%, 125%, 127%, 117%, 97%, 90%, 103% and 103%, respectively.

Specifically, the barrier factor TGM1 was compared to the samples of example 1 (shea butter 0% + jojoba oil 0%) after the treatment of the samples of example 4 (shea butter 5%), example 9 (jojoba oil 5%), example 12 (shea butter 5% + jojoba oil 5%), example 17 (shea butter 5% + olive oil 5%), example 18 (shea butter 5% + safflower seed oil 5%), example 19 (shea butter 5% + meadowfoam seed oil 5%), example 20 (shea butter 5% + rice bran oil 5%), example 21 (shea butter 5% + sunflower seed oil 5%), example 22 (safflower seed oil 5%), example 23 (olive oil 5%), example 24 (sunflower seed oil 5%), example 25 (meadow flower seed oil 5%) and positive control (glitazone), 134%, 126%, 176%, 170%, 176%, 201%, 161%, 160%, 201%, 156%, 146%, 149%, and 161%, respectively.

Specifically, the barrier factor CASP14 was compared to the samples of example 1 (shea butter 0% + jojoba oil 0%) after the treatment of the samples of example 4 (shea butter 5%), example 9 (jojoba oil 5%), example 12 (shea butter 5% + jojoba oil 5%), example 17 (shea butter 5% + olive oil 5%), example 18 (shea butter 5% + safflower seed oil 5%), example 19 (shea butter 5% + meadow flower seed oil 5%), example 20 (shea butter 5% + rice bran oil 5%), example 21 (shea butter 5% + sunflower seed oil 5%), example 22 (safflower seed oil 5%), example 23 (olive oil 5%), example 24 (sunflower seed oil 5%), example 25 (meadow flower seed oil 5%) and positive control (glitazone), 188%, 275%, 169%, 201%, 228%, 162%, 175%, 192%, 493%, 198%, 248%, 279%, and 330%, respectively.

Specifically, the barrier factor HMGCR was compared with the samples of example 1 (shea butter 0% + jojoba oil 0%) after the treatment of the samples of example 4 (shea butter 5%), the samples of example 9 (jojoba oil 5%), the samples of example 12 (shea butter 5% + jojoba oil 5%), the samples of example 17 (shea butter 5% + olive oil 5%), the samples of example 18 (shea butter 5% + safflower seed oil 5%), the samples of example 19 (shea butter 5% + meadowfoam seed oil 5%), the samples of example 20 (shea butter 5% + rice bran oil 5%), the samples of example 21 (shea butter 5% + sunflower seed oil 5%), the samples of example 22 (safflower seed oil 5%), the samples of example 23 (olive oil 5%), the samples of example 24 (sunflower seed oil 5%), the samples of example 25 (meadowfoam seed oil 5%) and the positive control (rosiglitazone), 170%, 223%, 226%, 208%, 253%, 213%, 199%, 192%, 242%, 188%, 202%, 175%, and 229%, respectively.

Specifically, the barrier factor AQP3 was compared to the samples of example 1 (shea butter 0% + jojoba oil 0%) after the treatment of the samples of example 4 (shea butter 5%), example 9 (jojoba oil 5%), example 12 (shea butter 5% + jojoba oil 5%), example 17 (shea butter 5% + olive oil 5%), example 18 (shea butter 5% + safflower seed oil 5%), example 19 (shea butter 5% + meadowfoam seed oil 5%), example 20 (shea butter 5% + rice bran oil 5%), example 21 (shea butter 5% + sunflower seed oil 5%), example 22 (safflower seed oil 5%), example 23 (olive oil 5%), example 24 (sunflower seed oil 5%), example 25 (meadow flower seed oil 5%) and positive control (glitazone), 112%, 105%, 141%, 125%, 133%, 116%, 163%, 172%, 148%, 99%, 116%, 97%, and 131%, respectively.

Specifically, the barrier factor ZO-1 was compared with the samples of example 1 (shea butter 0% + jojoba oil 0%) after the treatment of the samples of example 4 (shea butter 5%), the samples of example 9 (jojoba oil 5%), the samples of example 12 (shea butter 5% + jojoba oil 5%), the samples of example 17 (shea butter 5% + olive oil 5%), the samples of example 18 (shea butter 5% + safflower seed oil 5%), the samples of example 19 (shea butter 5% + meadowfoam seed oil 5%), the samples of example 20 (shea butter 5% + rice bran oil 5%), the samples of example 21 (shea butter 5% + sunflower seed oil 5%), the samples of example 22 (safflower seed oil 5%), the samples of example 23 (olive oil 5%), the samples of example 24 (sunflower seed oil 5%), the samples of example 25 (meadow flower seed oil 5%) and the positive control (glitazone), 143%, 151%, 160%, 138%, 147%, 128%, 180%, 190%, 203%, 131%, 170%, 134% and 246%, respectively.

In conclusion, the sample of example 4 (shea butter 5%) promoted the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, facilitating barrier formation, and had skin moisturizing and barrier repair promoting effects. Example 9 sample (jojoba oil 5%) promoted the expression of FLG, LOR, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, facilitated barrier formation, and had skin moisturizing and barrier repair promoting efficacy. Example 12 samples (shea butter 5% + jojoba oil 5%) promoted the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, favoured barrier formation, and had skin moisturizing and barrier repair promoting efficacy. Example 17 samples (Shea butter 5% + olive oil 5%) promoted the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, favoured barrier formation, with skin moisturizing and barrier repair promoting efficacy. Example 18 samples (shea butter 5% + safflower seed oil 5%) promoted the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, favouring barrier formation, with skin moisturizing and barrier repair promoting efficacy. Example 19 samples (Shea butter 5% + Shea butter 5%) promoted the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, favoured barrier formation, and had skin moisturizing and barrier repair promoting efficacy. Example 20 samples (shea butter 5% + rice bran oil 5%) promoted the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, favoured barrier formation, and had skin moisturizing and barrier repair promoting efficacy. Example 21 samples (shea butter 5% + sunflower seed oil 5%) promoted the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, favouring barrier formation, with skin moisturizing and barrier repair benefits. Example 22 sample (safflower seed oil 5%) promoted the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, facilitated barrier formation, and had skin moisturizing and barrier repair promoting effects. Example 23 sample (olive oil 5%) promoted the expression of FLG, LOR, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, facilitated barrier formation, had skin moisturizing and barrier repair efficacy. Example 24 sample (sunflower seed oil 5%) promoted the expression of FLG, LOR, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, facilitated barrier formation, had skin moisturizing and barrier repair benefits. Example 25 sample (meadowfoam seed oil 5%) promoted the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR and ZO-1 barrier factors, facilitated barrier formation, had skin moisturizing and barrier repair efficacy.

Application example

The composition containing the plant fruit oil and/or the plant seed oil can be used as an intermediate raw material or a efficacy additive for preparing medicines or skin external preparations, and the skin external preparations are preferably cosmetic compositions, including but not limited to cream, lotion, jelly, lotion, essence, facial masks, eye cream, aerosol (cleansing foam), spray, shower gel, massage oil, facial cleanser and other dosage forms.

The weight percentage of the composition comprising the plant fruit oil and/or the plant seed oil in the skin external preparation is 0.001-100% (w/w); preferably 0.01-60% (w/w); most preferably 0.01% to 40% (w/w).

The following are specific application examples of the compositions comprising vegetable fruit oil and/or vegetable seed oil obtained in examples 3, 7, 9, 20 and 23 to skin external preparations, and formulations and preparation methods of these formulations. The specific application examples are as follows:

application example 1: preparation of face cream

Application example 2: preparation of the emulsion

Application example 3: preparation of eye cream

Application example 4: preparation of facial mask

Application example 5: preparation of essence

Application example 6: examples 2 to 28 in the present invention can also be used alone as a skin external preparation alone.

Claims

1. A composition comprises vegetable oil and fat, wherein the vegetable oil and fat comprises vegetable fruit oil and vegetable seed oil,

wherein the vegetable fruit oil is selected from: shea butter, olive fruit oil, sweet almond oil, avocado fruit oil, sea buckthorn fruit oil, coconut fruit oil, orange fruit oil, bergamot fruit oil, citron fruit oil, tomato fruit oil, rosa canina fruit oil, pepper fruit oil, myrtle fruit oil, vanilla planifolia fruit oil, anise fruit oil, maoregano fruit oil, juniper berry oil, satsuma mandarin fruit oil, star palm fruit oil, dog rose fruit oil, coriander fruit oil, or combinations thereof;

wherein the plant seed oil is selected from: jojoba seed oil, safflower seed oil, meadowfoam seed oil, sunflower seed oil, rice bran oil, macadamia nut seed oil, castor bean oil, peony seed oil, grape seed oil, grapefruit seed oil, apple seed oil, pomegranate seed oil, watermelon seed oil, pumpkin seed oil, linseed oil, rapeseed oil, sesame seed oil, glass chicory seed oil, burkholderia walnut seed oil, grass cotton seed oil, tea seed oil, Sichuan valley seed oil, papaya seed oil, raspberry seed oil, monkey bushy seed oil, calendula seed oil, bitter tree seed oil, moringa seed oil, pecan seed oil, perilla seed oil, babassu seed oil, white lupin seed oil or a combination thereof,

wherein the composition comprises at least 0.5 wt.% vegetable fat based on the total weight of the composition.

2. The composition of claim 1, comprising a plant fruit oil and a plant seed oil in a weight ratio of plant fruit oil to plant seed oil of from 2:8 to 8: 2.

3. The composition of claim 1, wherein the composition further comprises a natural surfactant selected from the group consisting of: lecithin, cholesterol, lanolin, tea saponin, protein, saponins, saccharides, alkyl polyglycoside or their combination.

4. The composition of claim 3, wherein the natural surfactant is hydrogenated lecithin.

5. The composition according to any one of claims 3 to 4, wherein the weight ratio of the vegetable oil or fat to the natural surfactant is from 1:1 to 20: 1.

6. The composition of claim 5, wherein the weight ratio of the vegetable oil to the natural surfactant is from 10:1 to 20: 1.

7. Use of a composition according to claim 1 for enhancing the barrier function of skin.

8. Use according to claim 7, wherein the enhancement of skin barrier function is achieved by increasing skin barrier activity and/or promoting expression of skin barrier related factors.

9. Use of the composition according to claim 1 for the preparation of a medicament and/or a skin external preparation for enhancing the barrier function of the skin.

10. The use according to claim 9, wherein the pharmaceutical and/or dermatological preparations for external use comprise 0.001 to 100% by weight of the composition.