CN114177119A

CN114177119A - Composition containing vegetable fruit oil and fat and application thereof

Info

Publication number: CN114177119A
Application number: CN202111527427.3A
Authority: CN
Inventors: 殷庆飞; 张瑶瑶
Original assignee: Shanghai Jahwa United Co Ltd
Current assignee: Shanghai Jahwa United Co Ltd
Priority date: 2021-12-14
Filing date: 2021-12-14
Publication date: 2022-03-15

Abstract

The invention provides a composition containing vegetable fruit oil, wherein the vegetable fruit oil is selected from the following group: shea butter, olive fruit oil, sweet almond oil, avocado fruit oil, sea buckthorn fruit oil, coconut fruit oil, citrus junos fruit oil, bergamot fruit oil, citron fruit oil, tomato fruit oil, rosa canina fruit oil, pepper fruit oil, myrtle fruit oil, vanilla planifolia fruit oil, anise fruit oil, ruined palm fruit oil, juniper berry oil, satsuma mandarin fruit oil, star palm fruit oil, dog rose fruit oil, coriander fruit oil, or combinations thereof, the composition comprising at least 0.5 wt.% of vegetable fruit oil, based on the total weight of the composition. The invention also relates to the application of the composition containing the plant fruit oil and fat in enhancing the skin barrier function and/or in preparing medicines and/or skin external preparations for enhancing the skin barrier function.

Description

Composition containing vegetable fruit oil and fat and application thereof

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to a composition containing plant fruit oil and fat and application thereof in the aspect of skin barrier function.

Background

The skin is the organ with the largest area of the human body, is the first natural defense line covered on the surface of the human body, has the maximum function of protection, can effectively protect various external stimuli, and can regulate and control the moisture loss of the skin from the inside, which is the barrier function of the skin. Then, with the increasing living standard, people have higher and higher makeup requirements on themselves, and in the process of pursuing beauty, a plurality of phenomena of skin barrier damage caused by improper skin care, improper repair after medical and artistic work, skin inflammation and the like appear. The oil can form an oil film barrier on the surface of the skin, so that external adverse factors cannot cause stimulation to the skin, and the effects of protecting the skin, inhibiting the evaporation of water in the skin and preserving moisture are achieved.

According to different sources and chemical components of the grease, the grease can be mainly divided into: vegetable oil and fat, animal oil and fat, mineral oil and synthetic oil and fat, the vegetable oil and fat is close to the structure of human skin fat membrane due to the skin-friendly texture, has good permeability, deeply permeates and nourishes the skin, and is popular with a plurality of skin care products. The vegetable oil and fat used for cosmetic mainly come from fruit, seed and embryo of plant, and part of the vegetable comes from other parts of plant such as leaf, bark, root, petal and stamen. The oils and fats containing plant fruits are mainly shea butter (Butyrospermum parkii) fruit fat (also known as shea butter), olive (Olea europaea) fruit oil (also known as olive oil), apricot (Prunus armeniaca) kernel oil (also known as sweet almond oil), avocado (Persea gratisma) fruit oil, sea buckthorn (Hippophae rhamnoides) fruit oil, coconut (Cocos nucifera) fruit oil, fragrant Citrus (Citrus junos) fruit oil, fragrant lemon (Citrus aurantium bergamia) fruit oil, citron (Citrus medica) fruit oil, tomato (Solanum lycopersicum) fruit oil, Rosa canina (Rosa canina) fruit oil, pepper (pepper nigrum) fruit oil, brazilian mahogany (Caryocar brasiliensis) fruit oil, Vanilla planifolia (Vanilla planifolia) fruit oil, anise (Pimpinella anisum) fruit oil, sorrel (Mauritia flexuosa) fruit oil, juniper (Juniperus communis) fruit oil, Litsea cubeba (Litsea cubeba) fruit oil, satsuma mandarin (Citrus unshiu) fruit oil, astrocarpus (astrocarpus vulgare) fruit oil, rose (Rosa multiflora) fruit oil, and coriander (coriander sativum) fruit oil.

Shea butter, also known as shea butter, has 3 colors, ivory, beige and yellow, the darker the color the better. Shea butter is a fruit directly extracted from a shea tree in africa, has soft and smooth texture, is the most effective natural moisturizing product, and is widely applied to the field of cosmetics. For example, the document Chenpeofeng, the application of Shea butter in cosmetic varieties, Fujian light textile [ J ],2003,12(175):1-4, indicates that Shea butter can be used not only as an emollient in skin care emulsions and creams to achieve a powerful moisturizing effect, but also in products such as lipstick, lip gloss and sun protection, and has good adhesion, softness and non-greasiness, and can assist in increasing the SPF (sun protection factor). In addition, the edible oil shea butter can be obtained by fractionation of shea butter, such as: the research of extraction process of bioactive substances in shea butter in the Bodhisaea of Huangxian, Chinese oil J, 2015,40(4):69-73 proposes that shea butter can be used as a substitute of cocoa butter for making chocolate, candy and the like. The shea butter has the advantages of being close to each index of sebum secretion oil of a human body, containing rich non-saponification components, being easy to be absorbed by the human body, preventing drying and cracking, further recovering and maintaining the natural elasticity of the skin, protecting the barrier function of the skin and playing a role in diminishing inflammation.

The olive oil is natural oil extracted from fresh olive fruits by a proper method, and is widely applied to the industries of medicine, daily chemicals and the like. For example, the literature is mazejora, the efficacy of natural vegetable oils in sunscreen cosmetics has been studied, and it is proposed in china oil [ J ],2021,46(1):71-75 that polyphenol components contained in olive oil have ultraviolet-absorbing and antioxidant effects, and also can eliminate facial wrinkles and prevent skin aging. The Wangcheng chapter, Chinese olive development process and industry prospect, and the biomass chemical engineering [ J ],2013,47(2):41-46 propose that olive oil can improve the digestive system, reduce cardiovascular diseases and prevent cancers, and the like, and meanwhile, oleic acid contained in olive oil can reduce blood pressure, balance blood fat and blood sugar. The olive oil has a slight skin feel, is very suitable for dry skin, contains squalene and fatty acid which can be quickly absorbed by the skin, effectively keeps the skin elastic and moist, contains abundant monounsaturated fatty acid, vitamin and phenolic substances, can eliminate facial wrinkles, prevents skin aging, and has the effects of protecting skin and hair, preventing rhagadia manus et pedis and the like.

The sweet almond oil can soften and condition skin, has good repairing and moisturizing effects on dry, cracked, itchy or irritated skin, and is particularly suitable for dry and sensitive skin.

Avocado oil is used as a cosmetic lubricant, and has effects in smoothing skin, promoting skin to be smooth, promoting skin cell regeneration, and repairing sunburn and dry skin.

Coconut oil is saturated fat, has stable lipid, is not easy to oxidize to generate free radical attack, has strong antioxidation capability to help human body to prevent the generation of free radicals, can be used for beautifying and protecting skin, has emulsification stabilizing effect and antioxidation, and can make cosmetics more uniform and fine.

The skin barrier, generally referred to as the physical barrier of the skin, includes the stratum corneum layer of the epidermis of the skin and the sebaceous membrane located on the surface of the stratum corneum layer. The stratum corneum is located at the outermost layer of the epidermis and has a "brick-wall structure", wherein "bricks" refer to keratinocytes and "mortar" refers to intercellular lipids. In the process of skin barrier formation, more than 200 genes are highly associated with the skin barrier. For example, the barrier factors involved in the formation of the cornified envelope, silk polyprotein (FLG), Loricrin (LOR), endothelin (IVL), transglutaminase 1(TGM1), and the barrier moisturizing-related factors caspase 14(CASP14), HMGCoA reductase (HMGCR), aquaporin 3(AQP3), and the keratinocyte intercellular junction-related factor zonulin 1 (ZO-1).

In particular, Filaggrin (FLG) is an important constituent protein of the cornified envelope of the outer layer of the epidermis of the skin, mainly present in the stratum granulosum and stratum lucidum, and an important constituent structure of the skin barrier; and can be further degraded into hydrophilic free amino acids and water-absorbing derivatives, which can help skin store water and maintain skin water content, and has moisturizing effect. Loricrin (LOR) is a major component of the cornified envelope and plays an important role in the barrier function of the epidermis. Endothelin (IVL) is a marker protein for keratinocyte differentiation, mainly expressed in the upper part of the acanthocyte layer and the granular layer, and is located in the outer layer of the cornified envelope, and is covalently bound to a ceramide containing a hydroxyl group — OH, thereby linking the lipid matrix and the keratinocytes to each other to function. The characteristic resistance and insolubility of the cornified envelope is based on the very stable isopeptide bond formation catalyzed by transglutaminase 1(TGM 1).

HMGCoA reductase (HMGCR) is a rate-limiting enzyme for synthesizing cholesterol, exists in endoplasmic reticulum, catalyzes and synthesizes methyl dihydroxy pentanoic acid, then generates squalene through multi-step reactions such as decarboxylation, phosphorylation and the like, and finally converts the squalene into cholesterol after cyclization. Aquaporin 3(AQP3) is the most abundant aquaporin subtype expressed by AQPs family, has spatial hierarchy in the expression quantity of epidermis, and is mainly expressed in the basal layer, the acanthocyte layer and the granular layer of human epidermis and gradually disappears to the stratum corneum. This spatial distribution is consistent with the moisture content distribution of the skin: the water content of the basal layer and the upper part of the basal layer is about 75%, while the stratum corneum is only about 10% -15%. The high expression of AQP3 in the basal lamina membranacea can promote the transportation of water, glycerol and urea, so that the extracellular environment of the basal lamina membranacea is closer to a neutral equilibrium state; the closer to the stratum corneum, the less AQP3 was expressed, the more the water content decreased significantly, and the environment in the skin became increasingly acidic. In addition, AQP3 plays an important role in the transport of keratin glycerol, and AQP3 transports endogenous glycerol into the epidermis, which directly or indirectly affects the skin moisturizing effect.

Tight junctions are an important component of the physical barrier, mostly present in the epidermal layer of the skin, laterally at the top of the adjacent intercellular spaces. Tight junctions are composed of different types of transmembrane and intracellular cytoplasmic proteins. Transmembrane proteins such as sluice protein 1(CLDN1), Occludin (OCLN), etc.; cytoplasmic proteins include mainly zonula occludens-1 (ZO-1). ZO-1 is a cytoplasmic protein belonging to the family of membrane-associated guanylate kinase proteins, which aggregates atresia and sluice proteins via extracellular signaling pathways to form a basic cell junction band; ZO-1 is a cytoplasmic protein most characteristic of skin barrier function.

Plant biomimetic lipid technology (Phyto Bionic Sebum, PBS technology for short): a plurality of natural vegetable oils are selected, natural lipid components in a skin barrier structure are simulated through scientific proportioning, unsaturated fatty acids such as linolenic acid, linoleic acid and the like which are necessary for a skin cuticle are supplemented, lipid components lacking in the skin are supplemented, and the lipid components are compatible with sebum, so that the skin barrier is repaired quickly, and the skin is enabled to be in a healthy, moist and glossy state.

Wherein: the vegetable oil and fat is mainly derived from, but not limited to, plant fruits (e.g., shea butter, olive oil, almond oil, avocado oil, sea buckthorn oil, coconut oil, orange oil, bergamot oil, citron oil, tomato oil, rosa canina oil, piper oil, brazil nut oil, vanilla oil, anise oil, ruined palm oil, juniper berry oil, wenzhou mandarin fruit oil, star palm fruit oil, wild rose fruit oil, and coriander fruit oil), seeds (e.g., jojoba seed oil, safflower seed oil, meadowfoam nut oil, sunflower seed oil, macadamia, castor bean seed oil, peony seed oil, grape seed oil, apple seed oil, pomegranate seed oil, pumpkin seed oil, linseed oil, rapeseed oil, sesame seed oil, glass seed oil, brazil nut oil, berba seed oil, etc.), sesame seed oil, olive seed oil, apricot seed oil, olive seed oil, avocado seed oil, olive, Kapok seed oil, tea seed oil, Sichuan valley seed oil, papaya seed oil, raspberry seed oil, Adonis amurensis seed oil, calendula seed oil, bitter tree seed oil, moringa oleifera seed oil, pecan seed oil, perilla seed oil, babassu seed oil, white lupin seed oil, and the like) and germ (for example: rice germ oil, wheat germ oil, oat germ oil, corn germ oil, etc.), and also other parts such as leaves, barks, roots, petals, pistils, etc., which are partially derived from plants.

The invention unexpectedly discovers that the plant fruit oil (such as shea butter, olive oil and the like) has skin barrier related efficacy, can improve the skin barrier activity and promote the expression of skin barrier related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1. Therefore, the composition comprising the vegetable fruit oil can be applied to medicines and skin external preparations as a efficacy additive.

Disclosure of Invention

In one aspect, the present invention provides a composition comprising vegetable fruit oil, wherein the vegetable fruit oil is selected from the group consisting of: shea butter, olive fruit oil, sweet almond oil, avocado fruit oil, sea buckthorn fruit oil, coconut fruit oil, citrus junos fruit oil, bergamot fruit oil, citron fruit oil, tomato fruit oil, rosa canina fruit oil, pepper fruit oil, myrtle fruit oil, vanilla planifolia fruit oil, anise fruit oil, ruined palm fruit oil, juniper berry oil, satsuma mandarin fruit oil, star palm fruit oil, dog rose fruit oil, coriander fruit oil, or combinations thereof, the composition comprising at least 0.5 wt.% of vegetable fruit oil, based on the total weight of the composition.

In a preferred embodiment, the composition comprising vegetable fruit oil further comprises a natural surfactant selected from the group consisting of: lecithin, cholesterol, lanolin, tea saponin, protein, saponins, saccharides, alkyl polyglycoside or their combination. In a more preferred embodiment, the natural surfactant is hydrogenated lecithin.

In a preferred embodiment, the weight ratio of the vegetable fruit oil to the natural surfactant in the composition is from 1:1 to 20: 1. In a more preferred embodiment, the weight ratio of the vegetable fruit oil to the natural surfactant in the composition is from 10:1 to 20: 1.

In another aspect, the present invention also relates to the use of a composition comprising vegetable fruit oil for enhancing the barrier function of skin. In a preferred embodiment, the enhancement of the skin barrier function is achieved by increasing the skin barrier activity and/or promoting the expression of skin barrier-associated factors.

In yet another aspect, the present invention relates to the use of a composition comprising vegetable fruit oil for the preparation of a medicament and/or a skin external preparation for enhancing the barrier function of the skin. In a preferred embodiment, the pharmaceutical product and/or the external preparation for skin comprises 0.001 to 100% by weight of the composition.

Detailed Description

The invention relates to a composition containing plant fruit oil and fat and application thereof, and the composition is found to have the effects of improving skin barrier activity and promoting the expression of skin barrier related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 in research. Therefore, the composition containing the vegetable fruit oil can be used as an efficacy additive for preparing medicines and/or skin external preparations for enhancing the skin barrier function.

To provide a more concise description, some of the quantitative representations presented herein are not modified by the term "about". It is understood that each quantity given herein is intended to refer to the actual given value, regardless of whether the term "about" is explicitly used, and also to refer to the approximation to such given value that would reasonably be inferred by one of ordinary skill in the art, including approximations due to experimental and/or measurement conditions for such given value.

To provide a more concise description, some quantitative expressions are recited herein as a range from about an X amount to about a Y amount. It should be understood that when a range is recited, the range is not limited to the upper and lower limits recited, but includes the entire range from about the X amount to about the Y amount or any amount therebetween.

Plant fruit oil

The plant fruit oil in the invention is also called plant fruit oil, which is mainly from plant fruits, seeds and embryos, and also has parts from other parts of plants such as leaves, barks, roots, petals, stamens and the like. Vegetable fruit oils include, but are not limited to, Butyrospermum parkii (Butyrospermum parkii) fruit fat (also known as shea butter), Olea europaea (Olea europaea) fruit oil (also known as olive oil), apricot (Prunus armeniaca) kernel oil (also known as sweet almond oil), avocado (Persea gratisma) fruit oil, Hippophae rhamnoides (Hippophae rhamnoides) fruit oil, Cocos nucifera fruit oil, Citrus junos (Citrus junos) fruit oil, Citrus bergamia (Citrus aurantium bergamia) fruit oil, Citrus medica (Citrus bergamia) fruit oil, Lycopersicon esculentum (Solomonisco sium) fruit oil, Citrus canina (Rosa canina) fruit oil, Pepper (Pipernium) fruit oil, Myrothecium brazianum (Carriera) fruit oil, Citrus depressa (Pimpinella) fruit oil, Citrus depressa officinalis fruit oil, Citrus depressa (Pimpinella) fruit oil, Citrus depressa (Pimpinella) fruit oil, and Pimpinella oil, Fruit oil of Rosa multiflora (Rosa multiflora) and fruit oil of Coriandrum sativum (Coriandrum sativum).

For example, shea butter has indexes close to those of sebum secretion oil of human body, contains rich non-saponified components, is easy to be absorbed by human body, can prevent drying and cracking, further recovers and maintains the natural elasticity of skin, protects the barrier function of skin, and also has the function of diminishing inflammation. The olive oil has a slight skin feel, is very suitable for dry skin, contains squalene and fatty acid which can be quickly absorbed by the skin, effectively keeps the skin elastic and moist, contains abundant monounsaturated fatty acid, vitamin and phenolic substances, can eliminate facial wrinkles, prevents skin aging, and has the effects of protecting skin and hair, preventing rhagadia manus et pedis and the like. The sweet almond oil can soften and condition skin, has good repairing and moisturizing effects on dry, cracked, itchy or irritated skin, and is particularly suitable for dry and sensitive skin. Avocado oil is used as a cosmetic lubricant, and has effects in smoothing skin, promoting skin to be smooth, promoting skin cell regeneration, and repairing sunburn and dry skin. Coconut oil is saturated fat, has stable lipid, is not easy to oxidize to generate free radical attack, has strong antioxidation capability to help human body to prevent the generation of free radicals, can be used for beautifying and protecting skin, has emulsification stabilizing effect and antioxidation, and can make cosmetics more uniform and fine.

The present invention has unexpectedly found that a composition comprising a plant fruit oil (e.g., shea butter, olive oil, etc.) has skin barrier-related efficacy, can increase skin barrier activity and promote the expression of skin barrier-related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1.

In some embodiments, the compositions of the present invention comprise from 0.1 to 100% by weight of vegetable fruit oil. In a preferred embodiment, the composition of the invention comprises from 0.1 to 50% by weight of vegetable fruit oil. In a preferred embodiment, the composition of the invention comprises from 0.1 to 20% by weight of vegetable fruit oil. In a preferred embodiment, the composition of the invention comprises from 0.5 to 10% by weight of vegetable fruit oil.

Natural surfactant

The compositions of the present invention may also comprise natural surfactants. Natural surfactants are mostly derived from animal and plant bodies and are relatively complex high molecular organic substances. Because of its strong hydrophilicity, it can form an emulsion. These substances usually have a high viscosity, which is beneficial to emulsion stability.

Natural surfactants included in the compositions of the present invention include, but are not limited to: lecithin, cholesterol, lanolin, tea saponin, protein, saponins, saccharides, alkyl polyglycoside, etc. Lecithin exists in biological cells, such as animal egg, brain, etc., and plant seed or embryo, and yolk phospholipid is extracted from yolk; soybean is rich in lecithin. Lecithin has physiological activities of emulsification, dispersion, oxidation resistance and the like, and is a natural excellent surfactant and an important emulsifier.

The hydrogenated lecithin has strong hydrophilicity and moisture retention, has strong affinity to skin and mucous membrane, and can play the roles of moisture retention, emulsification, dispersion and the like when being used in a formula of cosmetics; as a surfactant, the oil-water-free emulsion can also condition the skin to achieve a good oil-water balance effect, and the hydrogenated lecithin can be used for developing advanced cosmetic products such as skin care cream, hand cream, lipstick, sunscreen oil and the like.

In some embodiments, the compositions of the present invention comprise from 0.1 to 20 wt% of a natural surfactant. In a preferred embodiment, the composition of the invention comprises from 0.1 to 10% by weight of natural surfactant. In a preferred embodiment, the composition of the invention comprises 0.1 to 5 wt% of a natural surfactant. In a preferred embodiment, the composition of the invention comprises 0.5 to 2 wt% of a natural surfactant.

In a preferred embodiment, the weight ratio of the vegetable fruit oil to the natural surfactant in the composition of the invention is from 1:1 to 20: 1. In a more preferred embodiment, the weight ratio of the vegetable fruit oil to the natural surfactant in the composition is from 10:1 to 20: 1.

Drug and/or external preparation for skin

The composition comprising the vegetable fruit oil can be applied to a pharmaceutical product and/or a skin external preparation for enhancing the skin barrier function.

In some embodiments, the pharmaceutical product is selected from: tablets, capsules, emulsions, suspensions, powders, granules, solutions, and various pharmaceutical dosage forms known in the art. Different amounts are added according to different types of dosage forms.

In some embodiments, the external skin agent is selected from: face cleaning lotion, cosmetic water, lotion, cream, jelly and facial mask. Different amounts are added according to different types of preparations.

The external preparation for skin is a general concept of all ingredients generally used for the external part of skin, and may be, for example, a cosmetic composition. The cosmetic composition can be basic cosmetics, face makeup cosmetics, body makeup cosmetics, hair care cosmetics and the like, and the dosage form of the cosmetic composition is not particularly limited and can be reasonably selected according to different purposes. The cosmetic composition also contains different cosmetically acceptable media or matrix excipients according to different dosage forms and purposes.

The external preparation for skin contains a dermatologically acceptable carrier or vehicle (e.g., lotion, cream, ointment, cleanser, etc.). One of ordinary skill in the art will be able to select a carrier that will dissolve or disperse the components in the concentrations described above, based on common general knowledge in the art.

One of ordinary skill in the art will be able to select suitable carriers, including for example water, alcohols, oils, and the like, based on the common general knowledge and their ability to dissolve or disperse in the active ingredient at the concentrations most suitable for treatment.

The skin external preparation of the present invention may be in the form of a topical application product, which can be externally applied to the skin, and can be prepared by those ordinary techniques well known in the art. The carrier may take any of a variety of practical forms such as a cream, dressing, gel, lotion, ointment or liquid comprising the applied and rinsed-off composition and incorporated into a carrier of material such as a dry or wet spread, hydrogel matrix, or adhesive (or non-adhesive) patch by methods well known in the art. Preferably, the carrier is a gel or a lotion that adds moisture, or a spread in dry or wet form.

Typical carriers include emulsions comprising water and/or an alcohol and an emollient such as hydrocarbon oils and waxes, silicone oils, hyaluronic acid, vegetable, animal or marine fats or oils, glyceride derivatives, fatty acids, or fatty acid esters or alcohols or alcohol ethers, lanolin and its derivatives, polyols or esters, wax esters, sterols, phospholipids and the like, and typically emulsifiers (nonionic, cationic or anionic), although some emollients inherently have emulsifying properties. In addition, these same components may be formulated into creams, gels, or solid sticks using different ratios of their components and/or by incorporating thickeners such as gums or other forms of hydrocolloids.

The skin external agent of the present invention may contain additional components commonly found in skin care compositions, such as emollients, skin conditioning agents, emulsifiers, preservatives, antioxidants, fragrances, chelating agents, and the like, as long as they are physically and chemically compatible with the other components of the skin external agent and do not affect the effect of the vegetable fruit oil of the present invention.

In some embodiments of the external preparation for skin of the present invention, one or more preservatives may be used. Suitable preservatives include p-hydroxyacetophenone, C1-C4 alkyl parabens, and phenoxyethanol. Preservatives are used in amounts of about 0.5 to about 2 weight percent, preferably about 0.5 to 1 weight percent, based on the total weight of the composition.

In one example of the external preparation for skin of the present invention, one or more antioxidants may be used. Suitable antioxidants include Butylated Hydroxytoluene (BHT), ascorbyl palmitate (BHA), butylated hydroxyanisole, phenyl-alpha-naphthylamine, hydroquinone, gallopropyl, nordihydroguaiaretic acid, vitamin E or derivatives of vitamin E, vitamin C and its derivatives, calcium pantothenate, green tea extract and mixed polyphenols, and mixtures of the above. The antioxidant is used in an amount ranging from about 0.02 to 0.5 weight percent, more preferably from about 0.002 to 0.1 weight percent, based on the total weight of the composition.

In one example of the external preparation for skin of the present invention, one or more emollients may be used, functioning as a lubricant by their ability to remain on the surface of the skin or in the stratum corneum, to reduce flaking, and to improve the appearance of the skin. Typical emollients include fatty esters, fatty alcohols, mineral oils, polyether siloxane copolymers, and the like. Examples of suitable emollients include, without limitation, polypropylene glycol ("PPG") -15 stearyl ether, PPG-10 cetyl ether, Steareth-10, Oleth-8, PPG-4 lauryl ether, vitamin E acetate, lanolin, cetyl alcohol, cetearyl ethylhexanoate, cetearyl alcohol, glyceryl stearate, octyl hydroxystearate, dimethylpolysiloxane, and combinations thereof. Cetyl alcohol, cetearyl ethylhexanoate, cetostearyl alcohol, glyceryl stearate and combinations thereof are preferred. When used, the emollient is present in an amount ranging from about 0.1 to about 30 weight percent, preferably from about 1 to about 30 weight percent, based on the total weight of the composition.

In one example of the external preparation for skin of the present invention, one or more moisturizers may also be used. Humectants, also known as humectants, help to increase the effectiveness of the emollient, reduce flaking, stimulate the removal of compositional scales, and improve skin feel. Polyhydric alcohols may be used as humectants, including, but not limited to, glycerin, polyalkylene glycols, alkylene polyols and derivatives thereof, including butylene glycol, propylene glycol, dipropylene glycol, polyglycerol, polyethylene glycol and derivatives thereof, sorbitol, hydroxypropyl sorbitol, hexylene glycol, 1, 3-dibutylene glycol, 1,2, 6-hexanetriol, ethoxylated glycerin, propoxylated glycerin, and combinations thereof. When used, the humectant is present in an amount of about 0.1 to about 20 weight percent, preferably about 1 to about 15 weight percent, based on the total weight of the composition.

In one example of the external preparation for skin of the present invention, one or more emulsifiers may be used. The emulsifier may be used in an effective stabilizing amount range. Preferably, the emulsifier is used in an amount of about 1.0 to about 10.0 wt%, more preferably about 3.0 to about 6.0 wt%, based on the total weight of the composition. Any emulsifier that is compatible with the components of the composition may be used. Suitable emulsifiers include stearic acid, cetyl alcohol, glyceryl stearate, lecithin, stearyl alcohol, Steareth-2, Steareth-20, acrylic/C10-30 alkanol acrylate crosspolymer, and combinations thereof.

In one example of the external preparation for skin of the present invention, one or more pH adjusting agents may be used. The pH adjusting agent useful in the skin external preparation of the present invention includes tromethamine. When used, the pH adjusting agent is used in an amount of about 0.1 to about 2 weight percent, preferably about 0.1 to about 1 weight percent, based on the total weight of the composition.

In one embodiment of the present invention, the external preparation for skin comprises acrylic acid/C10-30 alkanol acrylate crosspolymer, glycerin, p-hydroxyacetophenone, glyceryl stearate and lecithin, cetostearyl alcohol, cetearyl ethyl hexanoate, tromethamine or a combination thereof.

In some embodiments of the present invention, the composition of the present invention is used in an amount of 0.001% to 100% (w/w), preferably 0.01% to 60% (w/w), and more preferably 0.01% to 40% (w/w) in the skin external preparation.

Examples

The invention will be further illustrated by the following specific examples. It should be noted that the examples are given solely for the purpose of illustration and are not to be construed as limitations on the scope of the invention, as many insubstantial modifications and variations of the invention may be made by those skilled in the art in light of the above teachings. Test methods in which specific conditions are not specified in the following examples are generally carried out under conventional conditions or under conditions recommended by the manufacturer. All percentages and parts are by weight unless otherwise indicated.

The experimental materials used in the following examples include:

butyrospermum parkii (Butyrum parkii) fruit fat, a trade name of refined shea butter, available from basf New materials, Inc.;

olive (Olea europaea) fruit oil, sold under the trade name refined olive oil, available from Textron tenica, s.l.u.;

hydrogenated lecithin, trade name hydrogenated lecithin, was purchased from NIKKO CHEMICALS co.

The experimental apparatus used in the examples included:

weighing a balance: PB4002-N type by METTLER TOLEDO;

a pH meter: SEVENMULTI model METTLER TOLEDO;

a water bath kettle: an electric heating constant temperature water bath kettle of HWS28 model of Shanghai constant science and technology Limited;

a homogenizer: POLYTRON PT 3100D;

a desk type stirrer: IKA @ EUROSTAR, power control-visc.

Example 1: preparation of the composition

Weighing 0.5 part by mass of hydrogenated lecithin in a beaker, adding 99.5 parts by mass of deionized water, and uniformly stirring for later use.

Example 2: preparation of composition containing vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 0.5 part by mass of shea butter in a beaker, heating to 75-80 ℃ while stirring, then weighing 99 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 3: preparation of composition containing vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 2.5 parts by mass of shea butter in a beaker, heating to 75-80 ℃ while stirring, then weighing 97 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 4: preparation of composition containing vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 5 parts by mass of shea butter in a beaker, heating to 75-80 ℃ while stirring, then weighing 94.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 5: preparation of composition containing vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 7.5 parts by mass of shea butter in a beaker, heating to 75-80 ℃ while stirring, then weighing 92 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 6: preparation of composition containing vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 10 parts by mass of shea butter in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 7: preparation of composition containing vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin and 5 parts by mass of olive oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 94.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 8: preparation of composition containing vegetable fruit oil

Weighing 0.5 part by mass of hydrogenated lecithin, 5 parts by mass of shea butter and 5 parts by mass of olive oil in a beaker, heating to 75-80 ℃ while stirring, then weighing 89.5 parts by mass of deionized water at 75-80 ℃ in the beaker, starting homogenizing at 3000 r/min for 5-10 min for later use.

Example 9: preparation of composition containing vegetable fruit oil

Weighing 100 parts by mass of shea butter and testing.

Test example 1: 3D epidermal recombination model tissue viability test

The 3D epidermal recombination model is a human 3D epidermal model and can simulate the structure of each layer of the epidermis differentiated by keratinocytes. According to the 3D epidermal recombination model tissue viability testing method, firstly, a certain damage is caused to the model by using a skin irritant SDS, and then, a proper amount of sample prepared by the embodiment is covered on the surface of the damaged model for 24 hours to repair. After 24 hours, the samples were washed away and tested for tissue viability recovery using the MTT method. The skin barrier repair ability of the example samples can be evaluated according to the tissue viability recovery.

1. Test materials

1.1 cells: the skin model is a 3D epidermal skin model

(EpiSkin)。

1.2 reagent:

3D skin model maintenance medium, 3D skin model detection medium (EpiSkin); sodium Dodecyl Sulfate (SDS) (Sigma); calcium and magnesium containing dpbs (gibco); mtt (sigma); isopropanol and concentrated hydrochloric acid (national drug group).

1.3 Main Equipment

CO₂An incubator HERAcell 240i, a biological safety cabinet 1300SERIES A2, a microplate reader Multiskan Mk3 (Thermo); forward pipettor M25 (Gilson); 50mL Multipette plus (Eppendorf) continuous applicator tip; skin punch (area 0.38 cm)²)。

2. The experimental method comprises the following steps:

3D recombinant epidermal model reception and resuscitation

Receiving the 3D epidermal recombination model, checking the integrity of the package, checking the number of models, checking the maintenance medium, and detecting the color and volume of the medium. And after the error is confirmed, starting to perform model recovery.

The resuscitation operation is performed in a biosafety cabinet. A number of 12-well plates were taken, 2 ml/well of maintenance medium was added to the first row, then the 3D recombinant epidermis model was removed with forceps, the surface gel was carefully removed with a cotton swab, placed in the wells containing maintenance medium, and allowed to resuscitate for 24 hours. One 12-well plate can resuscitate 4 3D recombinant epidermal models. The culture conditions are as follows: 5% CO₂And culturing in a constant-temperature incubator at 37 ℃.

3D recombination epidermis model damage treatment and sample adding product

A1.5% (w/v) Sodium Dodecyl Sulfate (SDS) solution was prepared using a 1 XPhosphate buffer solution (DPBS).

The model was divided into a blank control group (3 replicates without any treatment), a negative control group (3 replicates with 1.5% SDS) and sample groups of examples 1-6 and 8-9 (3 replicates per sample).

2 ml/well of maintenance medium was added to the second row of the 12-well plate, and then the 3D recombinant epidermal model located in the first row was transferred to the corresponding second row with tweezers.

50 microliters of 1.5% SDS solution was added dropwise to the surface of the negative control and sample groups of examples 1-6 and 8-9 using a pipette, and the coverage was secured to be uniform, and incubated at room temperature for 15 minutes. After 15 minutes, immediately wash with 1 × DPBS and gently wipe the residual liquid with a cotton swab. Then, 15. mu.l of the sample was applied to the surface of the lesion-treated (1.5% SDS) sample group model by using a piston displacement pipette (M25) and treated for 24 hours. All models were placed in a cell incubator under the following culture conditions: 5% CO₂And culturing in a constant-temperature incubator at 37 ℃.

3D recombinant epidermal model viability test

A 3 mg/ml stock solution of thiazole blue (MTT) was prepared with 1 xdpbs and the MTT stock solution was diluted to 0.3 mg/ml in the test medium. 2 ml/well of diluted MTT solution was added in the third row of the 12-well plate.

The sample on the surface of the model was washed off (not directly aligned for washing, but aligned to the side wall of the skin model support frame) by pipetting 1 XDPBS with a 50ml continuous pipette, and then pipetting the residual liquid with a cotton swab into a 12-well plate in the third line MTT solution. Finally placing the mixture into a cell culture box, and placing the cell culture box at 37 ℃ and 5% CO₂Was incubated in the incubator of (1) for 3 hours.

Acidic isopropanol was prepared and 1.8 ml of hydrochloric acid was added to 500 ml of isopropanol. A corresponding number of 1.5 ml microcentrifuge tubes (EP tubes) were molded and 500. mu.l of the acidic isopropanol was added to each tube. After the epidermal tissue was incubated in MTT for 3 hours, the epidermal tissue was removed with a punch, separated from the underlying collagen matrix with two small-gauge elbow forceps, and inverted, and then transferred together with the collagen matrix to a 1.5 ml EP tube containing acidic isopropanol. The EP tube was shaken and mixed on a vortex, and placed in a refrigerator at 4 ℃ for 72 hours for color extraction. Meanwhile, prepare an EP tube containing 1 ml of acid isopropanol, as the blank hole when the enzyme-linked immunosorbent assay detects.

The 1.5 ml EP tube containing the acid isopropanol and the skin model was taken out from the 4 ℃ freezer, the solution was mixed by shaking, 150 μ l of the extraction solution was taken out per tube and transferred to a 96-well plate, and 3 times of the extraction solution were taken out and added to the 96-well plate. Meanwhile, 3-6 wells of acidic isopropanol were prepared as blank control during detection, excluding background optical density (OD value). The OD was read at a wavelength of 570 nm. Relative activity of each group of models (blank control 100%) was calculated according to the following formula:

3. result judgment

The blank control group is an undamaged group, and the relative activity is 100%; the relative activity of the negative control group is controlled to be 40-60% compared with that of the blank control group; a higher relative activity in the sample group than in the negative control group indicates that the sample group has the ability to promote barrier repair, and a lower relative activity in the sample group than in the negative control group indicates that the sample group does not have the ability to promote barrier repair.

Table 1: MTT method for detecting relative activity condition of each group of tissues

As shown in table 1, the average relative viability of the skin model tissue of the blank control group was 100%, and the average relative viability of the skin model tissue of the negative control group (1.5% SDS treatment) was 41.0%. The average relative viability of the skin model tissue was 48.4% for the sample of example 1 (shea butter 0%), 64.1% for the sample of example 2 (shea butter 0.5%), 75.5% for the sample of example 3 (shea butter 2.5%), 75.3% for the sample of example 4 (shea butter 5%), 76.1% for the sample of example 5 (shea butter 7.5%), 76.1% for the sample of example 6 (shea butter 10%), 69.0% for the sample of example 8 (shea butter 5% + olive oil 5%), and 68.4% for the sample of example 9 (shea butter 100%) (see table 1).

In conclusion, shea butter in examples 2-6 and 9 has a good tissue repair ability at a concentration ranging from 0.5 to 100% by weight.

Test example 2: 3D recombinant skin epidermal model barrier-related gene expression test

The 3D epidermal recombination model is a human 3D epidermal model and can simulate the structure of each layer of the epidermis differentiated by keratinocytes. Covering a proper amount of samples on an epidermis model for 24 hours, then collecting total ribonucleic acid (RNA) of epidermis tissues, and quantitatively detecting related genes of the skin barrier by adopting an RNA reverse transcription technology and a gene microarray technology so as to evaluate the skin barrier moisturizing and repairing capacity of the samples.

1. Test materials

1.1 cells: the skin model is a 3D epidermal skin model

(EpiSkin)。

1.2 reagent:

3D skin model maintenance medium (EpiSkin); calcium and magnesium containing dpbs (gibco); isopropanol, 75% absolute ethanol (national pharmaceutical group); DEPC water (tiangen organisms); total RNA extraction reagent trizol (thermofisher); reverse transcription Kit iScript cDNA Synthesis Kit and fluorescent real-time quantitative PCR reagent iTaq^TM Universal

Green Supermix(Bio-rad)。

1.3 Main Equipment

CO₂Incubator HERACell 240i, biosafety cabinet 1300SERIES A2, ultramicro UV spectrophotometer NanoDrop ONE (Thermo); gene amplificationAn instrument C1000 Touch, a real-time quantitative PCR instrument CFX Connect (Bio-rad); high speed refrigerated centrifuge MIKRO 220r (hittich); forward pipettor M25 (Gilson); 50mL Multipette plus (Eppendorf) continuous applicator tip; skin punch (area 0.38 cm)²)。

2. The experimental method comprises the following steps:

3D recombinant epidermal model reception and resuscitation

3D recombinant epidermal model sample treatment

The model was divided into the sample groups of examples 1,4, 7, 8 (3 replicates per sample) and the positive control group (rosiglitazone, 3 replicates).

A piston displacement pipettor (M25) was used to add 15 microliters of rosiglitazone solution (100 micromoles in 1 x DPBS) to the positive control group, 15 microliters of sample was added to the sample group, and the samples were gently spread evenly over the skin model surface using a suction nozzle, in triplicate for each sample. All models were placed in a cell incubator and incubated for 24 hours. The culture conditions are as follows: 5% CO₂And culturing in a constant-temperature incubator at 37 ℃.

3D recombinant epidermal model Collection of Total RNA samples

The sample on the surface of the model was washed off by sucking 1 XDPBS with a 50ml continuous sample applicator (sample cannot be directly aligned for washing, and the skin model support needs to be aligned for washing)Frame side walls) and then swab off the residual liquid. The epidermal tissue was cut with a punch, separated from the underlying collagen base with two small-gauge bent tweezers, placed in an EP tube containing 1 ml of total RNA extraction reagent (TRIzol), and repeatedly tapped with 1 ml of a rnase-free pipette tip until only the transparent stratum corneum remained. Subsequently, 0.2 ml of chloroform was added to the original EP tube, vigorously shaken, and centrifuged at a high speed at a low temperature for 15 minutes. Taking 500 microliters of the upper aqueous phase by using a pipette nozzle without RNase, adding equal volume of precooled (minus 20 ℃) isopropanol, reversing, mixing uniformly, and centrifuging at a high speed for 10 minutes at a low temperature. The centrifuged RNA forms a white flaky precipitate at the bottom of the EP tube, the precipitate is rinsed twice with 75% ethanol, residual ethanol is removed by a centrifuge and a suction nozzle, and the precipitate is opened and dried to be transparent at room temperature. With 40. mu.l RNase-free H₂Dissolving O, and mixing. The RNA concentration was measured again using a microspectrophotometer Nanodrop and the integrity of the RNA was checked by agarose gel electrophoresis. The dissolved total RNA of the epidermal model tissue is used for subsequent experiments or is stored for a long time at minus 80 ℃ after being subpackaged.

Quantitative detection of skin barrier related gene by RT-qPCR method

Taking 1 microgram of total RNA for reverse transcription, and adopting a reverse transcription Kit iScript cDNA Synthesis Kit for reverse transcription to obtain cDNA.

The reverse transcription system is as follows:

the reverse transcription procedure was as follows: 5 minutes at 25 ℃; 30 minutes at 42 ℃; 5 minutes at 85 ℃.

Real-time quantification of Polymerase Chain Reaction (PCR) reagent iTaq using fluorescence^TM Universal

Green Supermix is used for carrying out fluorescence real-time quantitative detection, and the expression condition of messenger RNA (mRNA) of the barrier related gene is analyzed.

The reaction system is as follows:

the reaction procedure was as follows: at 95 ℃ for 30 seconds; at 95 deg.C, 15 seconds, 60 deg.C, 30 seconds, 40 cycles; 65-95 deg.C, each time by 0.5 deg.C (melting curve).

The primer sequences used in this example were designed as follows:

by using

The gene expression of the sample group and the positive control group (rosiglitazone) of example was analyzed for changes relative to the gene expression of the control group of example 1.

3. Result judgment

Example 1 the gene expression levels of the sample groups were taken as 100%, and each group compared to it, more than 100%, indicated up-regulation of gene expression, and less than 100%, indicated down-regulation of gene expression. FLG, LOR and other genes are up-regulated, which indicates that the sample group has the capacity of moisturizing and promoting barrier repair; genes such as FLG, LOR were down-regulated, indicating that this sample group did not have the ability to moisturize and promote barrier repair.

Table 2: expression of skin Barrier factor after treatment of Each sample

As shown in Table 2, the relative gene expression level of the sample (Shea butter 0%) of example 1 was 100% as a control, and the gene expression levels of the samples of examples 4, 7 and 8 and the positive control sample were compared to obtain the corresponding percentage.

Specifically, the barrier factor FLG was 222%, 205%, 265% and 362% compared to example 1 (shea butter 0%) after treatment of the example 4 sample (shea butter 5%), the example 7 sample (olive butter 5%), the example 8 sample (shea butter 5% + olive butter 5%) and the positive control (rosiglitazone), respectively.

Specifically, the barrier factors LOR were 240%, 216%, 242% and 351% after treatment of the example 4 sample (shea butter 5%), the example 7 sample (olive butter 5%), the example 8 sample (shea butter 5% + olive butter 5%) and the positive control (rosiglitazone), respectively, compared to the example 1 (shea butter 0%) sample.

Specifically, the barrier factor IVL was 113%, 97%, 107% and 103% after treatment of the example 4 sample (shea butter 5%), the example 7 sample (olive butter 5%), the example 8 sample (shea butter 5% + olive butter 5%) and the positive control (rosiglitazone), respectively, compared to the example 1 sample (shea butter 0%).

Specifically, the barrier factor TGM1 was 134%, 156%, 170% and 161% compared to the example 1 sample (shea butter 0%) after treatment of the example 4 sample (shea butter 5%), the example 7 sample (olive oil 5%), the example 8 sample (shea butter 5% + olive oil 5%) and the positive control (rosiglitazone), respectively.

Specifically, the barrier factors CASP14 were 188%, 198%, 201% and 330% after treatment of the example 4 sample (shea butter 5%), the example 7 sample (olive oil 5%), the example 8 sample (shea butter 5% + olive oil 5%) and the positive control (rosiglitazone), respectively, compared to the example 1 sample (shea butter 0%).

In particular, the barrier factors HMGCR were 170%, 188%, 208% and 229% after treatment of the example 4 sample (shea butter 5%), the example 7 sample (olive oil 5%), the example 8 sample (shea butter 5% + olive oil 5%) and the positive control (rosiglitazone), respectively, compared to the example 1 sample (shea butter 0%).

Specifically, the barrier factors AQP3 were 112%, 99%, 138% and 131% compared to the example 1 (shea butter 0%) sample after treatment of the example 4 sample (shea butter 5%), the example 7 sample (olive oil 5%), the example 8 sample (shea butter 5% + olive oil 5%) and the positive control (rosiglitazone), respectively.

Specifically, the barrier factor ZO-1 was 143%, 131%, 229% and 246% after treatment of the example 4 sample (shea butter 5%), the example 7 sample (olive oil 5%), the example 8 sample (shea butter 5% + olive oil 5%) and the positive control (rosiglitazone), respectively, compared to the example 1 (shea butter 0%) sample.

In conclusion, the sample of example 4 (shea butter 5%) promoted the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, facilitating barrier formation, and had skin moisturizing and barrier repair promoting effects. Example 7 sample (olive oil 5%) promoted the expression of FLG, LOR, TGM1, CASP14, HMGCR and ZO-1 barrier factors, favoured barrier formation, with skin moisturizing and barrier repair promoting efficacy. Example 8 samples (Shea butter 5% + olive oil 5%) promoted the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, favouring barrier formation, with skin moisturizing and barrier repair promoting efficacy. Meanwhile, analysis of the relative expression amounts of FLG, LOR, TGM1, CASP14, HMGCR and ZO-1 in the samples of example 4 (shea butter 5%), example 7 (olive oil 5%) and example 8 (shea butter 5% + olive oil 5%) showed that shea butter 5% had a synergistic effect with olive oil 5%.

Application example

The composition containing the plant fruit oil can be used as an intermediate raw material or a functional additive for preparing medicines or skin external preparations, and the skin external preparations are preferably cosmetic compositions, including but not limited to preparation of products in dosage forms of cream, lotion, gel, lotion, essence, facial mask, eye cream, aerosol (cleaning foam), spray, shower gel, massage oil, facial cleanser and the like.

The weight percentage of the composition containing the plant fruit oil in the skin external preparation is 0.001-100% (w/w); preferably 0.01-60% (w/w); more preferably 0.01-40% (w/w).

The following are specific examples of application of the compositions containing vegetable fruit oils and fats obtained in examples 2 to 6 to external preparations for skin, and the formulations and preparation methods of these preparations. The specific application examples are as follows:

application example 1: preparation of face cream

Application example 2: preparation of the emulsion

Application example 3: preparation of eye cream

Application example 4: preparation of facial mask

Application example 5: preparation of essence

Application example 6: examples 2 to 9 of the present invention can also be used alone as a skin external preparation alone.

Claims

1. A composition comprising vegetable fruit oil and fat, wherein the vegetable fruit oil and fat is selected from the group consisting of: shea butter, olive fruit oil, sweet almond oil, avocado fruit oil, sea buckthorn fruit oil, coconut fruit oil, citrus junos fruit oil, bergamot fruit oil, citron fruit oil, tomato fruit oil, rosa canina fruit oil, pepper fruit oil, myrtle fruit oil, vanilla planifolia fruit oil, anise fruit oil, ruined palm fruit oil, juniper berry oil, satsuma mandarin fruit oil, star palm fruit oil, dog rose fruit oil, coriander fruit oil, or combinations thereof, the composition comprising at least 0.5 wt.% of vegetable fruit oil, based on the total weight of the composition.

2. The composition of claim 1, further comprising a natural surfactant.

3. The composition of claim 2, wherein the natural surfactant is selected from the group consisting of: lecithin, cholesterol, lanolin, tea saponin, protein, saponins, saccharides, alkyl polyglycoside or their combination.

4. The composition of claim 2, wherein the natural surfactant is hydrogenated lecithin.

5. The composition of any one of claims 2-4, wherein the weight ratio of the vegetable fruit oil to the natural surfactant is from 1:1 to 20: 1.

6. The composition of claim 5, wherein the weight ratio of the vegetable fruit oil to the natural surfactant is from 10:1 to 20: 1.

7. Use of a composition according to claim 1 for enhancing the barrier function of skin.

8. Use according to claim 7, wherein the enhancement of skin barrier function is achieved by increasing skin barrier activity and/or promoting expression of skin barrier related factors.

9. Use of the composition according to claim 1 for the preparation of a medicament and/or a skin external preparation for enhancing the barrier function of the skin.

10. The use according to claim 9, wherein the pharmaceutical and/or dermatological preparations for external use comprise 0.001 to 100% by weight of the composition.