JP2011042644A - Composition for beautification - Google Patents
Composition for beautification Download PDFInfo
- Publication number
- JP2011042644A JP2011042644A JP2010091615A JP2010091615A JP2011042644A JP 2011042644 A JP2011042644 A JP 2011042644A JP 2010091615 A JP2010091615 A JP 2010091615A JP 2010091615 A JP2010091615 A JP 2010091615A JP 2011042644 A JP2011042644 A JP 2011042644A
- Authority
- JP
- Japan
- Prior art keywords
- solution
- composition
- extract
- flower
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 68
- 239000000284 extract Substances 0.000 claims abstract description 52
- 230000009759 skin aging Effects 0.000 claims abstract description 10
- 241000736199 Paeonia Species 0.000 claims description 30
- 235000006484 Paeonia officinalis Nutrition 0.000 claims description 29
- 239000002537 cosmetic Substances 0.000 claims description 29
- JSUVCAQGWVUMPD-QXUQJYLISA-N Arjuna Natural products O([C@H]([C@@H](O)C=O)[C@H]1[C@H](O)COC(=O)c2c(c(O)c(O)c(O)c2)-c2c(O)c(O)c3OC(=O)c4c(c(O)c(O)c5OC(=O)c2c3-c45)-c2c(O)c(O)c(O)cc2C(=O)O1)C(=O)c1cc(O)c(O)c(O)c1 JSUVCAQGWVUMPD-QXUQJYLISA-N 0.000 claims description 28
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 claims description 27
- 108010003272 Hyaluronate lyase Proteins 0.000 claims description 18
- 102000001974 Hyaluronidases Human genes 0.000 claims description 18
- 229960002773 hyaluronidase Drugs 0.000 claims description 18
- 230000037319 collagen production Effects 0.000 claims description 17
- 230000001737 promoting effect Effects 0.000 claims description 15
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 claims description 13
- -1 foundation Substances 0.000 claims description 13
- 230000002292 Radical scavenging effect Effects 0.000 claims description 10
- 230000002000 scavenging effect Effects 0.000 claims description 9
- 235000013305 food Nutrition 0.000 claims description 7
- 239000006210 lotion Substances 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 5
- 239000006071 cream Substances 0.000 claims description 4
- 229940079593 drug Drugs 0.000 claims description 3
- 239000000839 emulsion Substances 0.000 claims description 3
- 238000010410 dusting Methods 0.000 claims description 2
- 230000005764 inhibitory process Effects 0.000 claims description 2
- 244000236658 Paeonia lactiflora Species 0.000 abstract description 3
- 235000008598 Paeonia lactiflora Nutrition 0.000 abstract description 3
- 241001474977 Palla Species 0.000 abstract description 2
- 244000071109 Terminalia arjuna Species 0.000 abstract description 2
- 235000000538 Terminalia arjuna Nutrition 0.000 abstract description 2
- 241000328973 Tilia miqueliana Species 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 51
- 238000012360 testing method Methods 0.000 description 34
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 33
- 238000006243 chemical reaction Methods 0.000 description 32
- 238000002360 preparation method Methods 0.000 description 29
- 239000012490 blank solution Substances 0.000 description 22
- 230000000694 effects Effects 0.000 description 21
- 239000012488 sample solution Substances 0.000 description 18
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 16
- 230000002401 inhibitory effect Effects 0.000 description 16
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 15
- 210000003491 skin Anatomy 0.000 description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 11
- 229940088598 enzyme Drugs 0.000 description 11
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 10
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 10
- 229920002674 hyaluronan Polymers 0.000 description 10
- 229960003160 hyaluronic acid Drugs 0.000 description 10
- 102000008186 Collagen Human genes 0.000 description 8
- 108010035532 Collagen Proteins 0.000 description 8
- 238000002835 absorbance Methods 0.000 description 8
- 229920001436 collagen Polymers 0.000 description 8
- 238000003756 stirring Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 238000000605 extraction Methods 0.000 description 7
- 239000002994 raw material Substances 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 150000003254 radicals Chemical class 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- BGNGWHSBYQYVRX-UHFFFAOYSA-N 4-(dimethylamino)benzaldehyde Chemical compound CN(C)C1=CC=C(C=O)C=C1 BGNGWHSBYQYVRX-UHFFFAOYSA-N 0.000 description 5
- 240000007313 Tilia cordata Species 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 210000004207 dermis Anatomy 0.000 description 5
- 210000002950 fibroblast Anatomy 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 239000007987 MES buffer Substances 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 4
- 235000008504 concentrate Nutrition 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000004215 skin function Effects 0.000 description 4
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 3
- 239000004327 boric acid Substances 0.000 description 3
- 239000003205 fragrance Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000007665 sagging Methods 0.000 description 3
- JUJBNYBVVQSIOU-UHFFFAOYSA-M sodium;4-[2-(4-iodophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate Chemical compound [Na+].C1=CC([N+](=O)[O-])=CC=C1N1[N+](C=2C=CC(I)=CC=2)=NC(C=2C(=CC(=CC=2)S([O-])(=O)=O)S([O-])(=O)=O)=N1 JUJBNYBVVQSIOU-UHFFFAOYSA-M 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 239000012086 standard solution Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 230000037303 wrinkles Effects 0.000 description 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 2
- WNWHHMBRJJOGFJ-UHFFFAOYSA-N 16-methylheptadecan-1-ol Chemical compound CC(C)CCCCCCCCCCCCCCCO WNWHHMBRJJOGFJ-UHFFFAOYSA-N 0.000 description 2
- XDOFQFKRPWOURC-UHFFFAOYSA-N 16-methylheptadecanoic acid Chemical compound CC(C)CCCCCCCCCCCCCCC(O)=O XDOFQFKRPWOURC-UHFFFAOYSA-N 0.000 description 2
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- GLEVLJDDWXEYCO-UHFFFAOYSA-N Trolox Chemical compound O1C(C)(C(O)=O)CCC2=C1C(C)=C(C)C(O)=C2C GLEVLJDDWXEYCO-UHFFFAOYSA-N 0.000 description 2
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 2
- 239000008351 acetate buffer Substances 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 230000002921 anti-spasmodic effect Effects 0.000 description 2
- 239000012298 atmosphere Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000000812 cholinergic antagonist Substances 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 210000003953 foreskin Anatomy 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000002453 shampoo Substances 0.000 description 2
- 238000000967 suction filtration Methods 0.000 description 2
- WCDDVEOXEIYWFB-VXORFPGASA-N (2s,3s,4r,5r,6r)-3-[(2s,3r,5s,6r)-3-acetamido-5-hydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4,5,6-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@@H]1C[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O)[C@H](O)[C@H]1O WCDDVEOXEIYWFB-VXORFPGASA-N 0.000 description 1
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- 229940058015 1,3-butylene glycol Drugs 0.000 description 1
- FRPZMMHWLSIFAZ-UHFFFAOYSA-N 10-undecenoic acid Chemical compound OC(=O)CCCCCCCCC=C FRPZMMHWLSIFAZ-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- MOMKYJPSVWEWPM-UHFFFAOYSA-N 4-(chloromethyl)-2-(4-methylphenyl)-1,3-thiazole Chemical compound C1=CC(C)=CC=C1C1=NC(CCl)=CS1 MOMKYJPSVWEWPM-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 244000215068 Acacia senegal Species 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 244000020518 Carthamus tinctorius Species 0.000 description 1
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- ZAFNJMIOTHYJRJ-UHFFFAOYSA-N Diisopropyl ether Chemical compound CC(C)OC(C)C ZAFNJMIOTHYJRJ-UHFFFAOYSA-N 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- AFSDNFLWKVMVRB-UHFFFAOYSA-N Ellagic acid Chemical compound OC1=C(O)C(OC2=O)=C3C4=C2C=C(O)C(O)=C4OC(=O)C3=C1 AFSDNFLWKVMVRB-UHFFFAOYSA-N 0.000 description 1
- ATJXMQHAMYVHRX-CPCISQLKSA-N Ellagic acid Natural products OC1=C(O)[C@H]2OC(=O)c3cc(O)c(O)c4OC(=O)C(=C1)[C@H]2c34 ATJXMQHAMYVHRX-CPCISQLKSA-N 0.000 description 1
- 229920002079 Ellagic acid Polymers 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 206010019280 Heart failures Diseases 0.000 description 1
- 208000035150 Hypercholesterolemia Diseases 0.000 description 1
- 239000002211 L-ascorbic acid Substances 0.000 description 1
- 235000000069 L-ascorbic acid Nutrition 0.000 description 1
- 241000134253 Lanka Species 0.000 description 1
- 241000219071 Malvaceae Species 0.000 description 1
- NTIZESTWPVYFNL-UHFFFAOYSA-N Methyl isobutyl ketone Chemical compound CC(C)CC(C)=O NTIZESTWPVYFNL-UHFFFAOYSA-N 0.000 description 1
- UIHCLUNTQKBZGK-UHFFFAOYSA-N Methyl isobutyl ketone Natural products CCC(C)C(C)=O UIHCLUNTQKBZGK-UHFFFAOYSA-N 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 241000124449 Paeonia obovata Species 0.000 description 1
- 241000124501 Paeonia obovata var. japonica Species 0.000 description 1
- 241001106477 Paeoniaceae Species 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 235000019482 Palm oil Nutrition 0.000 description 1
- 239000004264 Petrolatum Substances 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000010632 SOD assay Methods 0.000 description 1
- 241000555745 Sciuridae Species 0.000 description 1
- YIQKLZYTHXTDDT-UHFFFAOYSA-H Sirius red F3B Chemical compound C1=CC(=CC=C1N=NC2=CC(=C(C=C2)N=NC3=C(C=C4C=C(C=CC4=C3[O-])NC(=O)NC5=CC6=CC(=C(C(=C6C=C5)[O-])N=NC7=C(C=C(C=C7)N=NC8=CC=C(C=C8)S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)O)S(=O)(=O)O)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+] YIQKLZYTHXTDDT-UHFFFAOYSA-H 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- HVUMOYIDDBPOLL-XWVZOOPGSA-N Sorbitan monostearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O HVUMOYIDDBPOLL-XWVZOOPGSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 244000228451 Stevia rebaudiana Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 235000009330 Terminalia Nutrition 0.000 description 1
- 241001534869 Terminalia Species 0.000 description 1
- 241000907897 Tilia Species 0.000 description 1
- 235000015450 Tilia cordata Nutrition 0.000 description 1
- 235000002168 Tilia europaea Nutrition 0.000 description 1
- 235000021525 Tilia platyphyllos Nutrition 0.000 description 1
- 240000006909 Tilia x europaea Species 0.000 description 1
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 108010093894 Xanthine oxidase Proteins 0.000 description 1
- 102100033220 Xanthine oxidase Human genes 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 208000038016 acute inflammation Diseases 0.000 description 1
- 230000006022 acute inflammation Effects 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000001754 anti-pyretic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000002221 antipyretic Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000003788 bath preparation Substances 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 235000013871 bee wax Nutrition 0.000 description 1
- 239000012166 beeswax Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 235000019437 butane-1,3-diol Nutrition 0.000 description 1
- KHAVLLBUVKBTBG-UHFFFAOYSA-N caproleic acid Natural products OC(=O)CCCCCCCC=C KHAVLLBUVKBTBG-UHFFFAOYSA-N 0.000 description 1
- 239000000496 cardiotonic agent Substances 0.000 description 1
- 239000004203 carnauba wax Substances 0.000 description 1
- 235000013869 carnauba wax Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 229960000541 cetyl alcohol Drugs 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 229940110456 cocoa butter Drugs 0.000 description 1
- 235000019868 cocoa butter Nutrition 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 229920002055 compound 48/80 Polymers 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 239000008341 cosmetic lotion Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- SASYSVUEVMOWPL-NXVVXOECSA-N decyl oleate Chemical compound CCCCCCCCCCOC(=O)CCCCCCC\C=C/CCCCCCCC SASYSVUEVMOWPL-NXVVXOECSA-N 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000013024 dilution buffer Substances 0.000 description 1
- 239000002934 diuretic Substances 0.000 description 1
- 230000001882 diuretic effect Effects 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229960002852 ellagic acid Drugs 0.000 description 1
- 235000004132 ellagic acid Nutrition 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 229940075507 glyceryl monostearate Drugs 0.000 description 1
- 229940093915 gynecological organic acid Drugs 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 229940014041 hyaluronate Drugs 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- JMMWKPVZQRWMSS-UHFFFAOYSA-N isopropanol acetate Natural products CC(C)OC(C)=O JMMWKPVZQRWMSS-UHFFFAOYSA-N 0.000 description 1
- 229940011051 isopropyl acetate Drugs 0.000 description 1
- GWYFCOCPABKNJV-UHFFFAOYSA-N isovaleric acid Chemical compound CC(C)CC(O)=O GWYFCOCPABKNJV-UHFFFAOYSA-N 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 1
- 229960004705 kojic acid Drugs 0.000 description 1
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229940057995 liquid paraffin Drugs 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 description 1
- 229940078812 myristyl myristate Drugs 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 239000003346 palm kernel oil Substances 0.000 description 1
- 235000019865 palm kernel oil Nutrition 0.000 description 1
- 239000002540 palm oil Substances 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 229940056211 paraffin Drugs 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 235000019271 petrolatum Nutrition 0.000 description 1
- 229940066842 petrolatum Drugs 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- DQAKJEWZWDQURW-UHFFFAOYSA-N pyrrolidonecarboxylic acid Chemical class OC(=O)N1CCCC1=O DQAKJEWZWDQURW-UHFFFAOYSA-N 0.000 description 1
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000000932 sedative agent Substances 0.000 description 1
- 230000001624 sedative effect Effects 0.000 description 1
- 230000037380 skin damage Effects 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- 235000019983 sodium metaphosphate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 229950011392 sorbitan stearate Drugs 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- SFVFIFLLYFPGHH-UHFFFAOYSA-M stearalkonium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 SFVFIFLLYFPGHH-UHFFFAOYSA-M 0.000 description 1
- 239000011232 storage material Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000000475 sunscreen effect Effects 0.000 description 1
- 239000000516 sunscreening agent Substances 0.000 description 1
- 229940032362 superoxide dismutase Drugs 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 230000002381 testicular Effects 0.000 description 1
- DZKXJUASMGQEMA-UHFFFAOYSA-N tetradecyl tetradecanoate Chemical compound CCCCCCCCCCCCCCOC(=O)CCCCCCCCCCCCC DZKXJUASMGQEMA-UHFFFAOYSA-N 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 description 1
- GYDJEQRTZSCIOI-LJGSYFOKSA-N tranexamic acid Chemical compound NC[C@H]1CC[C@H](C(O)=O)CC1 GYDJEQRTZSCIOI-LJGSYFOKSA-N 0.000 description 1
- 229960000401 tranexamic acid Drugs 0.000 description 1
- ZIBGPFATKBEMQZ-UHFFFAOYSA-N triethylene glycol Chemical compound OCCOCCOCCO ZIBGPFATKBEMQZ-UHFFFAOYSA-N 0.000 description 1
- 229960002703 undecylenic acid Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 150000003712 vitamin E derivatives Chemical class 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
本発明は、コラーゲン産生促進用組成物、ヒアルロニダーゼ阻害用組成物、スーパーオキサイド消去用組成物およびＤＰＰＨ（1, 1-diphenyl-2-picrylhydrazyl）ラジカル消去用組成物、ならびにこれらの組成物を含む美容用組成物に関する。 The present invention relates to a composition for promoting collagen production, a composition for inhibiting hyaluronidase, a composition for eliminating superoxide and a composition for eliminating DPPH (1, 1-diphenyl-2-picrylhydrazyl) radicals, and a beauty comprising these compositions The present invention relates to a composition for use.
皮膚は、表皮、真皮および皮下組織の三層からなり、これらのうち真皮には、皮膚の弾力や保水性など、皮膚の機能維持に重要なコラーゲンおよびヒアルロン酸が多く含まれている。これらコラーゲンおよびヒアルロン酸は、真皮中に存在する線維芽細胞により産生される。 The skin consists of three layers of the epidermis, dermis and subcutaneous tissue, and among these, the dermis is rich in collagen and hyaluronic acid that are important for maintaining skin functions such as skin elasticity and water retention. These collagen and hyaluronic acid are produced by fibroblasts present in the dermis.
一方で、加齢や紫外線などのストレスにより線維芽細胞の機能が衰え、真皮におけるコラーゲンの変性および産生減少、ならびにヒアルロン酸の産生減少が起こる。特に、紫外線に曝露された皮膚においては、スーパーオキサイドやフリーラジカルなどが発生し、これらが皮膚の脂質やタンパク質に対する酸化反応を促進することにより、皮膚の急性炎症や真皮の障害などが引き起こされる。
その結果として、皮膚のしわ、たるみの発生、保水性や弾力などの皮膚機能の低下などのような皮膚の老化現象があらわれる。
On the other hand, the function of fibroblasts declines due to stress such as aging and ultraviolet rays, and collagen degeneration and production decrease in the dermis and hyaluronic acid production decrease occur. In particular, in the skin exposed to ultraviolet rays, superoxide, free radicals, and the like are generated, and these promote oxidative reaction to skin lipids and proteins, thereby causing acute inflammation of the skin, damage to the dermis, and the like.
As a result, skin aging phenomena such as skin wrinkles, sagging, and decreased skin functions such as water retention and elasticity appear.
このような皮膚の老化の防止および／または改善を目的として、糖、アミノ酸、有機酸、ピロリドンカルボン酸などを配合した組成物や失われたコラーゲンおよびヒアルロン酸を補うためにこれらを配合した組成物などが、これまでに開発されてきた。
しかしながら、これらは皮膚の保湿性を高めて、表皮の角質の状態を改善するだけであり、皮膚の老化を根本的に防止または改善するものではないため、いずれも満足できる効果を有していなかった。
Compositions containing sugars, amino acids, organic acids, pyrrolidone carboxylic acids, etc., and compositions containing these to supplement lost collagen and hyaluronic acid for the purpose of preventing and / or improving skin aging Etc. have been developed so far.
However, these only improve skin moisturization and improve the keratinous state of the epidermis, and do not fundamentally prevent or improve skin aging, so none have a satisfactory effect It was.
また、様々な植物体の抽出物などを配合した皮膚外用剤なども、これまでに開発されてきた（特許文献１および２参照）。しかしながら、これらはコラーゲンおよびヒアルロン酸の産生を促進し得るが、スーパーオキサイド、フリーラジカルなどに対しては有効ではなく、皮膚の老化の防止および／または改善には満足できるものではなかった。 In addition, skin external preparations containing various plant extracts have been developed so far (see Patent Documents 1 and 2). However, they can promote the production of collagen and hyaluronic acid, but are not effective against superoxide, free radicals, etc., and are not satisfactory in preventing and / or improving skin aging.
上記のような事情に鑑みて、本発明は、皮膚の老化の根本的な防止および／または改善に有用なコラーゲン産生促進用組成物、ヒアルロニダーゼ阻害用組成物、スーパーオキサイド消去用組成物およびＤＰＰＨラジカル消去用組成物、ならびにこれらの組成物のいずれかを含む美容用組成物を提供することを目的とする。 In view of the circumstances as described above, the present invention provides a composition for promoting collagen production, a composition for inhibiting hyaluronidase, a composition for eliminating superoxide, and a DPPH radical, which are useful for fundamentally preventing and / or improving skin aging. It is an object of the present invention to provide an erasing composition and a cosmetic composition comprising any of these compositions.
本発明者らは、鋭意研究の結果、ボダイジュ花、シャクヤク花およびテルミナリア アルジュナ（Terminalia arjuna）の各抽出物が、コラーゲン産生促進活性、ヒアルロニダーゼ阻害活性、スーパーオキサイド消去活性およびＤＰＰＨラジカル消去活性を有することを見出し、本発明を完成するに至った。 As a result of intensive studies, the present inventors have found that each extract of bodaiju flower, peony flower and Terminalia arjuna has collagen production promoting activity, hyaluronidase inhibitory activity, superoxide scavenging activity, and DPPH radical scavenging activity. As a result, the present invention has been completed.
すなわち、本発明によれば、ボダイジュ花、シャクヤク花およびテルミナリア アルジュナから選択される１種または２種以上の抽出物を含むコラーゲン産生促進用組成物、ヒアルロニダーゼ阻害用組成物、スーパーオキサイド消去用組成物およびＤＰＰＨラジカル消去用組成物、ならびにこれらの組成物を含む美容用組成物が提供される。 That is, according to the present invention, a composition for promoting collagen production, a composition for inhibiting hyaluronidase, or a composition for eliminating superoxide, comprising one or more extracts selected from bodaiju flowers, peony flowers and terminaria arjuna And DPPH radical scavenging compositions, and cosmetic compositions comprising these compositions are provided.
本発明のコラーゲン産生促進用組成物、ヒアルロニダーゼ阻害用組成物、スーパーオキサイド消去用組成物およびＤＰＰＨラジカル消去用組成物を使用または摂取することにより、線維芽細胞におけるコラーゲンの産生が促進され、ヒアルロニダーゼの活性が阻害されることによりヒアルロン酸が維持され、皮膚障害の原因であるスーパーオキサイドおよび／またはフリーラジカルが消去される効果が得られる。 By using or ingesting the composition for promoting collagen production, the composition for inhibiting hyaluronidase, the composition for eliminating superoxide and the composition for eliminating DPPH radical of the present invention, collagen production in fibroblasts is promoted, and hyaluronidase By inhibiting the activity, hyaluronic acid is maintained, and the effect of eliminating superoxide and / or free radicals that cause skin damage is obtained.
本明細書において、「皮膚の老化」とは、加齢や紫外線などの影響により引き起こされる皮膚のしわ、たるみの発生、保水性や弾力などの皮膚機能の低下などを意図する。 In the present specification, “skin aging” intends skin wrinkles and sagging caused by effects such as aging and ultraviolet rays, and reduction of skin functions such as water retention and elasticity.
ヒアルロニダーゼとは、皮膚、血管、関節などに存在するヒアルロン酸を分解する酵素である。該酵素の活性を阻害することにより、生体内でのヒアルロン酸の分解が抑制され、生体内のヒアルロン酸の量を一定に保ち得る。 Hyaluronidase is an enzyme that degrades hyaluronic acid present in skin, blood vessels, joints, and the like. By inhibiting the activity of the enzyme, the degradation of hyaluronic acid in the living body is suppressed, and the amount of hyaluronic acid in the living body can be kept constant.
スーパーオキサイド（Ｏ2 -）とは、活性酸素の一つであり、特に皮膚においては紫外線の曝露により発生することが知られている。一方、生体内にはスーパーオキサイドを消去する活性を有する酵素ＳＯＤ（Super Oxide Dismutase）が存在する。
スーパーオキサイド消去活性とは、ＳＯＤ様活性とも呼ばれ、スーパーオキサイドを分解して消去する活性を意味する。
Superoxide (O 2 − ) is one of active oxygens, and is known to be generated by exposure to ultraviolet rays, particularly in the skin. On the other hand, an enzyme SOD (Super Oxide Dismutase) having an activity of eliminating superoxide exists in the living body.
The superoxide erasing activity is also called SOD-like activity and means an activity of decomposing and erasing superoxide.
ＤＰＰＨは、安定なフリーラジカルとして知られ、ある物質が有するフリーラジカル消去活性を評価する際の標的物質として用いられる。したがって、ＤＰＰＨラジカル消去活性とは、ＤＰＰＨを用いて評価した、ある物質が有するフリーラジカルを消去する活性を意味する。 DPPH is known as a stable free radical and is used as a target substance when evaluating the free radical scavenging activity of a substance. Therefore, DPPH radical scavenging activity means the activity of scavenging free radicals possessed by a certain substance, evaluated using DPPH.
ボダイジュは、シナノキ科（Tiliaceae）の植物であり、その花を乾燥させた菩提樹花茶（リンデンフラワーティー）は、ヨーロッパを中心に１８世紀から飲用され、鎮静、鎮痙、発汗などの作用を有することが知られている。
本発明に用いられるボダイジュ花としては、ナツボダイジュ（Tilia platyphyllos Scop.）、フユボダイジュ（Tilia cordata Mill.）、セイヨウシナノキ（Tilia europaea L.）などのシナノキ科、シナノキ属（Tilia）の植物の花が挙げられるが、好ましくはこれらの花の花弁である。
Bodaiju is a plant of the Tiliaceae family, and its dried linden flower tea (Linden flower tea) has been drunk since the 18th century, mainly in Europe, and may have sedative, antispasmodic, and sweating effects. Are known.
As for the body squirrel flower used in the present invention, a plant flower of a linden family, such as Tilia platyphyllos Scop., Tilia cordata Mill., Tilia europaea L., and genus Tilia The petals of these flowers are preferred.
シャクヤクは、中国北西部、華北、西北地方から東シベリア、朝鮮半島にかけて自生するボタン科（Paeoniaceae）の植物であり、その根を乾燥させたものは、鎮痛、鎮痙、解熱、利尿などの作用を有する生薬として知られている。
本発明に用いられるシャクヤク花としては、シャクヤク（Paeonia lactiflora Pallas）、シベリアシャクヤク（Paeonia lactiflora）、ベニバナヤマシャクヤク（Paeonia obovata）、ヤマシャクヤク（Paeonia japonica）などのボタン科、ボタン属（Paeonia）の植物の花が挙げられるが、好ましくはこれらの花の花弁である。
Peonies are Paeoniaceae plants that grow naturally from northwestern China, North China, Northwest, East Siberia, and the Korean peninsula, and their dried roots have actions such as analgesic, antispasmodic, antipyretic, and diuretic. Known as a herbal medicine.
As the peony flowers used in the present invention, peony (Paeonia lactiflora Pallas), siberian peony (Paeonia lactiflora), safflower peony (Paeonia obovata), button family such as peony (Paeonia japonica), plant of genus (Paeonia) The petals of these flowers are preferred.
テルミナリア アルジュナとは、シクンシ科（Cambretaceae）モモタマナ属（Terminalia）の植物で、インド全域に植生し、約１８〜２８ｍの高さまで成長する落葉樹である。
テルミナリア アルジュナの樹皮は、インドでは強心剤として用いられており、また、冠動脈疾患、心不全および高コレステロール血症の治療、ならびに狭心症の疼痛緩和のために処方されてきた（特表２００８−５３７５３９号公報参照）。
本発明に用いられるテルミナリア アルジュナとしては、テルミナリア アルジュナの花、花穂、果実、果皮、茎、葉、枝、枝葉、幹、樹皮、根、根茎、根皮、種子などが挙げられるが、中でも根皮を用いるのが好ましい。
Terminaria Arjuna is a plant belonging to the genus Cambretaceae (Terminalia), and is a deciduous tree that grows up to a height of about 18-28 m throughout India.
Terminaria Arjuna bark has been used as a cardiotonic agent in India and has been prescribed for the treatment of coronary artery disease, heart failure and hypercholesterolemia, and pain relief for angina pectoris (No. 2008-537539). See the official gazette).
Examples of Terminaria arjuna used in the present invention include Terminaria arjuna flowers, flower spikes, fruits, pericarp, stems, leaves, branches, branches and leaves, stems, bark, roots, rhizomes, root barks, seeds, etc. Is preferably used.
本発明において原料として用いられるボダイジュ花、シャクヤク花およびテルミナリア アルジュナは、市場で入手することができる。
また、上記の各原料は、そのままの形態で用いてもよいが、乾燥および／または粉砕したものを用いるのが好ましい。
Bodaiju flowers, peony flowers and Terminaria arjuna used as raw materials in the present invention can be obtained on the market.
Each of the above raw materials may be used as it is, but it is preferable to use a dried and / or pulverized material.
本発明に用いられる原料を抽出するための溶媒は、特に限定されないが、水、親水性有機溶媒またはこれらの混液が好ましい。親水性有機溶媒としては、例えば、１価の低級アルコール（メタノール、エタノール、イソプロピルアルコール、ブタノールなど）、多価アルコール（グリセリン、エチレングリコール、プロピレングリコール、トリエチレングリコール、１，３−ブチレングリコールなど）、ケトン類（アセトン、メチルエチルケトン、メチルイソブチルケトンなど）、エステル類（酢酸エチル、酢酸イソプロピルなど）およびエーテル類（ジエチルエーテル、ジイソプロピルエーテルなど）が挙げられるが、中でもメタノールまたはエタノールが好ましい。 Although the solvent for extracting the raw material used for this invention is not specifically limited, Water, a hydrophilic organic solvent, or these liquid mixture is preferable. Examples of the hydrophilic organic solvent include monovalent lower alcohols (such as methanol, ethanol, isopropyl alcohol, and butanol), polyhydric alcohols (such as glycerin, ethylene glycol, propylene glycol, triethylene glycol, and 1,3-butylene glycol). , Ketones (acetone, methyl ethyl ketone, methyl isobutyl ketone, etc.), esters (ethyl acetate, isopropyl acetate, etc.) and ethers (diethyl ether, diisopropyl ether, etc.), among which methanol or ethanol is preferred.
抽出方法は、特に限定されないが、室温〜加熱下、溶媒に原料を浸漬して抽出する方法が好ましい。例えば、室温〜１００℃、好ましくは４０〜８０℃の温度で、撹拌下または非撹拌下に原料を浸漬することによって、抽出することができる。
抽出の際の溶媒量は、原料の量に応じて適宜設定できるが、通常、重量比で原料の１〜
３０倍量、好ましくは５〜２０倍量である。また、抽出時間は、特に限定されないが、撹拌下に浸漬する場合は１〜３時間程度、非撹拌下に浸漬する場合は５〜２４時間程度が適当である。
なお、上記の温度より低い温度で抽出することも可能であるが、その場合には、上記の抽出時間より長く浸漬することが好ましい。
また、抽出処理は、同一原料について１回のみ行ってもよいが、複数回、例えば、２〜５回程度行うこともできる。
The extraction method is not particularly limited, but a method of extracting by immersing the raw material in a solvent at room temperature to heating is preferable. For example, the extraction can be performed by immersing the raw material at room temperature to 100 ° C., preferably at 40 to 80 ° C., with stirring or without stirring.
The amount of solvent at the time of extraction can be appropriately set according to the amount of the raw material, but is usually 1 to
The amount is 30 times, preferably 5 to 20 times. Further, the extraction time is not particularly limited, but about 1 to 3 hours are appropriate for immersion under stirring, and about 5 to 24 hours are appropriate for immersion under non-agitation.
In addition, although it is also possible to extract at a temperature lower than said temperature, in that case, it is preferable to immerse longer than said extraction time.
Moreover, although the extraction process may be performed only once for the same raw material, it can be performed a plurality of times, for example, about 2 to 5 times.
上記の抽出方法により得られるボダイジュ花、シャクヤク花およびテルミナリア アルジュナの各抽出物は、そのままでもコラーゲン産生促進用組成物、ヒアルロニダーゼ阻害用組成物、スーパーオキサイド消去用組成物およびＤＰＰＨラジカル消去用組成物として用い得るが、必要に応じて、濃縮、希釈、濾過、脱臭、脱色、乾燥などの処理に付してもよい。また、各抽出物に含まれる特定の成分をカラム精製などにより、単離および／または濃縮してもよい。 The extracts of bodaiju flower, peony flower and terminaria arjuna obtained by the above extraction method are, as they are, a composition for promoting collagen production, a composition for inhibiting hyaluronidase, a composition for eliminating superoxide, and a composition for eliminating DPPH radicals. Although it can be used, if necessary, it may be subjected to a treatment such as concentration, dilution, filtration, deodorization, decolorization, and drying. Moreover, you may isolate and / or concentrate the specific component contained in each extract by column purification.
本発明のコラーゲン産生促進用組成物、ヒアルロニダーゼ阻害用組成物、スーパーオキサイド消去用組成物およびＤＰＰＨラジカル消去用組成物は、ボダイジュ花、シャクヤク花およびテルミナリア アルジュナから選択される１種または２種以上の抽出物を含むことを特徴とするが、中でもテルミナリア アルジュナの抽出物を含むことが好ましく、また、上記の３種の抽出物を全て含んでいてもよい。 The composition for promoting collagen production, the composition for inhibiting hyaluronidase, the composition for eliminating superoxide, and the composition for eliminating DPPH radical of the present invention are one or more selected from bodaiju flowers, peony flowers and terminaria arjuna Although it is characterized by including an extract, it is preferable to include an extract of Terminaria arjuna, and all the above three types of extracts may be included.
また、上記の各抽出物に、これらの抽出物が有する活性を損なわない範囲で、化粧品、医薬部外品、医薬品または食品（特定保健用食品、栄養機能食品を含む）などの技術分野において通常用いられている、他の成分を適宜配合してもよい。
そのような成分としては、例えば、油脂類（カカオ脂、ヤシ油、パーム核油など）、ロウ類（ミツロウ、カルナバロウなど）、炭化水素類（固形パラフィン、流動パラフィン、ワセリンなど）、脂肪酸類（オレイン酸、リノール酸、イソステアリン酸、ウンデシレン酸など）、アルコール類（エタノール、セチルアルコール、イソステアリルアルコールなど）、エステル類（ミリスチン酸ミリスチル、モノステアリン酸グリセリン、オレイン酸デシルなど）、界面活性剤（ラウリル硫酸ナトリウム、ステアリン酸ソルビタン、塩化ステアリルジメチルベンジルアンモニウムなど）、香料（合成香料、天然香料など）、甘味料（ショ糖、ステビアなど）、ｐＨ調整剤（炭酸水素ナトリウム、炭酸カリウムなど）、保存剤（安息香酸ナトリウム、ソルビン酸など）、保湿剤（グリセリン、コラーゲン、ヒアルロン酸など）、美白剤（Ｌ−アスコルビン酸、ハイドロキノンβ−Ｄ−グルコース、エラグ酸、コウジ酸、トラネキサム酸など）、粉体（顔料、色素、樹脂など）、紫外線吸収剤（パラメトキシケイ皮酸２エチルヘキシルなど）、紫外線散乱剤（酸化チタン、酸化亜鉛など）、増粘剤（アラビアゴム、メチルセルロースなど）、酸化防止剤（ビタミンＣ、ビタミンＥなど）、キレート剤（エデト酸二ナトリウム、クエン酸ナトリウム、メタリン酸ナトリウムなど）、滑沢剤（ステアリン酸マグネシウム、タルク、ポリエチレングリコールなど）、崩壊剤（デンプン類、結晶セルロースなど）などが挙げられる。
In addition, the above-mentioned extracts are usually used in technical fields such as cosmetics, quasi-drugs, pharmaceuticals or foods (including foods for specified health use and nutritional functional foods) as long as the activities of these extracts are not impaired. You may mix | blend the other component currently used suitably.
Examples of such components include oils and fats (cocoa butter, palm oil, palm kernel oil, etc.), waxes (e.g. beeswax, carnauba wax, etc.), hydrocarbons (solid paraffin, liquid paraffin, petrolatum, etc.), fatty acids (e.g. Oleic acid, linoleic acid, isostearic acid, undecylenic acid, etc.), alcohols (ethanol, cetyl alcohol, isostearyl alcohol, etc.), esters (myristyl myristate, glyceryl monostearate, decyl oleate, etc.), surfactants ( Sodium lauryl sulfate, sorbitan stearate, stearyldimethylbenzylammonium chloride, etc.), fragrance (synthetic fragrance, natural fragrance, etc.), sweetener (sucrose, stevia, etc.), pH adjuster (sodium bicarbonate, potassium carbonate, etc.), storage Agent (sodium benzoate, Rubic acid, etc.), moisturizers (glycerin, collagen, hyaluronic acid, etc.), whitening agents (L-ascorbic acid, hydroquinone β-D-glucose, ellagic acid, kojic acid, tranexamic acid, etc.), powders (pigments, dyes, Resin), ultraviolet absorbers (such as 2-ethylhexyl paramethoxycinnamate), ultraviolet scattering agents (such as titanium oxide and zinc oxide), thickeners (such as gum arabic and methylcellulose), antioxidants (vitamin C, vitamin E) Etc.), chelating agents (disodium edetate, sodium citrate, sodium metaphosphate, etc.), lubricants (magnesium stearate, talc, polyethylene glycol, etc.), disintegrating agents (starch, crystalline cellulose, etc.) .
本発明のコラーゲン産生促進用組成物、ヒアルロニダーゼ阻害用組成物、スーパーオキサイド消去用組成物およびＤＰＰＨラジカル消去用組成物は、これらの組成物を含む美容用組成物、好ましくは皮膚の老化の防止および／または改善するための美容用組成物として提供することができる。
本発明の美容用組成物を使用または摂取することにより、美容効果、特にしわ、たるみの発生、保水性や弾力などの皮膚機能の低下などの皮膚の老化を防止および／または改善する効果が期待できる。
The composition for promoting collagen production, the composition for inhibiting hyaluronidase, the composition for eliminating superoxide, and the composition for eliminating DPPH radicals of the present invention are cosmetic compositions containing these compositions, preferably preventing skin aging and It can be provided as a cosmetic composition for improving.
Use or ingestion of the cosmetic composition of the present invention is expected to have a cosmetic effect, in particular, an effect of preventing and / or improving skin aging such as generation of wrinkles, sagging, skin function such as water retention and elasticity. it can.
本発明の美容用組成物の形態としては、例えば、化粧水、クリーム、乳液、ファンデーション、エッセンス（エキス）、オイル、パック、マスク、口紅、打粉、クレンジングローション、シャンプー、リンス、コンディショナー、石鹸、ボディーシャンプー、浴用剤、日焼け止めローション、軟膏剤、パップ剤、散剤、錠剤、乳剤、トローチ剤、内用液剤、シロップ剤、ゲル剤、エアロゾル剤、飲料、菓子類などが挙げられる。 Examples of the cosmetic composition of the present invention include lotion, cream, emulsion, foundation, essence (extract), oil, pack, mask, lipstick, dusting, cleansing lotion, shampoo, rinse, conditioner, soap, body Shampoos, bath preparations, sunscreen lotions, ointments, poultices, powders, tablets, emulsions, troches, liquids for internal use, syrups, gels, aerosols, beverages, confectionery and the like can be mentioned.
上記の化粧品、医薬部外品、医薬品または食品（特定保健用食品、栄養機能食品を含む）などの形態の美容用組成物における、ボダイジュ花、シャクヤク花およびテルミナリア アルジュナの各抽出物の含量は、特に制限されず、該組成物の形態に応じて適宜選定することができる。
一例を挙げれば、上記の各抽出物の乾燥重量として、美容用組成物の全重量に対して、０．０００１〜５０重量％、好ましくは０．０００５〜４０重量％、より好ましくは０．００１〜３０重量％である。
In the cosmetic composition in the form of cosmetics, quasi-drugs, pharmaceuticals or foods (including foods for specified health use and nutritional functional foods), the content of each extract of bodaiju flower, peony flower and terminaria arjuna is It does not restrict | limit in particular, According to the form of this composition, it can select suitably.
As an example, the dry weight of each of the above extracts is 0.0001 to 50% by weight, preferably 0.0005 to 40% by weight, more preferably 0.001 based on the total weight of the cosmetic composition. ~ 30% by weight.
本発明の美容用組成物が皮膚外用剤である場合、上記の各抽出物の乾燥重量としての含量は、通常、該皮膚外用剤の全重量に対して、０．００１〜１０重量％であればよい。この含量が、０．００１重量％未満では十分な美容効果を期待できず、逆に、１０重量％を超えても超過分に見合った美容効果の増強は期待できず、不経済である。 When the cosmetic composition of the present invention is a skin external preparation, the content of each extract as a dry weight is usually 0.001 to 10% by weight based on the total weight of the skin external preparation. That's fine. If this content is less than 0.001% by weight, a sufficient cosmetic effect cannot be expected. Conversely, if it exceeds 10% by weight, the enhancement of the cosmetic effect corresponding to the excess cannot be expected, which is uneconomical.
また、本発明の美容用組成物を内服する場合の摂取量は、投与形態、年齢、体重などによって異なるが、上記の各抽出物の乾燥重量として、０．０１〜１０ｇ／日、好ましくは０．１〜３ｇ／日である。 In addition, the amount of intake when taking the cosmetic composition of the present invention varies depending on the dosage form, age, body weight, etc., but is 0.01 to 10 g / day, preferably 0, as the dry weight of each of the above extracts. .1-3 g / day.
以下に、本発明を実施例により詳細に説明するが、本発明はこれらの実施例に限定されるものではない。 EXAMPLES The present invention will be described in detail below by examples, but the present invention is not limited to these examples.
製造例１：ボダイジュ花、シャクヤク花およびテルミナリア アルジュナの抽出物の調製
以下のようにして、ボダイジュ花、シャクヤク花およびテルミナリア アルジュナの抽出物を得た。
（１）ボダイジュ花抽出物の調製
ボダイジュの花弁乾燥品（中国産）の粗粉砕物４０ｇを５０％（ｖ／ｖ）エタノール１０００ｍｌに投入し、５５℃で撹拌下に１時間浸漬した後、これを吸引ろ過して抽出液を得た。この抽出液を５５℃で減圧下に濃縮し、得られた濃縮液を凍結乾燥して、粉末状のボダイジュ花抽出物４．３ｇを得た。
Production Example 1: Preparation of Bodaiju Flower, Peonies Flower and Terminaria Arjuna Extracts As follows, bodaiju flower, peony flowers and Terminaria arjuna extracts were obtained.
(1) Preparation of bodaiju flower extract 40 g of coarsely ground bodaiju petal dried product (made in China) was put into 1000 ml of 50% (v / v) ethanol and immersed for 1 hour at 55 ° C. with stirring. Was subjected to suction filtration to obtain an extract. The extract was concentrated at 55 ° C. under reduced pressure, and the resulting concentrate was lyophilized to obtain 4.3 g of a powdered Bodaige flower extract.
（２）シャクヤク花抽出物の調製
シャクヤクの花弁乾燥品（中国産）の粗粉砕物３０ｇを５０％（ｖ／ｖ）エタノール５００ｍｌに投入し、５５℃で撹拌下に１時間浸漬した後、これを吸引ろ過して抽出液を得た。この抽出液を５５℃で減圧下に濃縮し、得られた濃縮液を凍結乾燥して、粉末状のシャクヤク花抽出物１１．２ｇを得た。
(2) Preparation of Peony Flower Extract 30 g of coarsely pulverized dried peony petal product (made in China) was put into 500 ml of 50% (v / v) ethanol and immersed for 1 hour at 55 ° C. with stirring. Was subjected to suction filtration to obtain an extract. The extract was concentrated at 55 ° C. under reduced pressure, and the resulting concentrate was lyophilized to obtain 11.2 g of a powdered peony flower extract.
（３）テルミナリア アルジュナ抽出物の調製
テルミナリア アルジュナの根皮乾燥品（スリランカ産）の粗粉砕物３０ｇを水５００ｍｌに投入し、５５℃で撹拌下に１時間浸漬した後、これを吸引ろ過して抽出液を得た。この抽出液を５５℃で減圧下に濃縮し、得られた濃縮液を凍結乾燥して、粉末状のテルミナリア アルジュナ抽出物６．１ｇを得た。
(3) Preparation of Terminaria arjuna extract 30 g of coarsely pulverized dried product of Terminaria arjuna root bark (produced in Sri Lanka) was poured into 500 ml of water and immersed for 1 hour at 55 ° C. with stirring. An extract was obtained. The extract was concentrated under reduced pressure at 55 ° C., and the resulting concentrate was lyophilized to obtain 6.1 g of a powdered Terminaria arjuna extract.
試験例１：コラーゲン産生促進活性試験
ボダイジュ花、シャクヤク花およびテルミナリア アルジュナの各抽出物のコラーゲン産生促進活性を、コラーゲンを特異的に染色する色素シリウスレッドを用いて、以下のように評価した。
（１）各抽出物の試料溶液の調製
ボダイジュ花抽出物、シャクヤク花抽出物およびテルミナリア アルジュナ抽出物を、それぞれジメチルスルホキシド（ＤＭＳＯ）に溶解して、各試料溶液（１０ｍｇ／ｍｌ）を調製した。
（２）細胞培養
正常ヒト新生児***線維芽細胞ＮＨＤＦ（ＮＢ）（倉敷紡績株式会社）を、正常ヒト皮膚繊維芽細胞増殖用低血清培地Ｍｅｄｉｕｍ １０６Ｓ（倉敷紡績株式会社）を用いて、３７℃、５％ＣＯ2雰囲気下で５日間培養した。
５日後、前記培地にて２×１０4ｃｅｌｌｓ／ｍｌに調製した細胞浮遊液を９６ウェルプレート（マイクロプレート：ＩＷＡＫＩ社製）に１００μｌ／ウェルで播種し、３７℃、５％ＣＯ2雰囲気下で４８時間培養した。
４８時間後、各ウェルの培地を除去し、これらのウェルに前記培地９９μｌと試料溶液１μｌとの混合液（試料終濃度１００μｇ／ｍｌ）を添加し、さらに４８時間培養した。また、対照ブランクとして、試料溶液に代えてＤＭＳＯ１μｌを前記培地９９μｌと混合した液を対照用のウェルに添加した。
Test Example 1: Collagen production promoting activity test Collagen production promoting activity of each extract of bodaiju flower, peony flower and terminaria arjuna was evaluated as follows using a pigment Sirius red that specifically stains collagen.
(1) Preparation of sample solution of each extract Bodaiju flower extract, peony flower extract and Terminaria arjuna extract were each dissolved in dimethyl sulfoxide (DMSO) to prepare each sample solution (10 mg / ml).
(2) Cell culture Normal human newborn foreskin fibroblasts NHDF (NB) (Kurashiki Boseki Co., Ltd.) were used at 37 ° C. using medium 106S (Kurashiki Boseki Co., Ltd.) The cells were cultured for 5 days in a 5% CO 2 atmosphere.
After 5 days, the cell suspension prepared to 2 × 10 4 cells / ml in the above medium was seeded in a 96-well plate (microplate: manufactured by IWAKI) at 100 μl / well, and at 37 ° C. in a 5% CO 2 atmosphere. Cultured for 48 hours.
After 48 hours, the medium in each well was removed, and a mixture of 99 μl of the medium and 1 μl of the sample solution (final sample concentration 100 μg / ml) was added to these wells, followed by further incubation for 48 hours. As a control blank, a solution prepared by mixing 1 μl of DMSO with 99 μl of the medium instead of the sample solution was added to the control well.
（３）被験反応液および対照ブランク液の調製
上記の工程（２）の培養後、各ウェルから培地を除去し、ＰＢＳ（−）で細胞を洗浄した後、シリウスレッド試薬（０．１％ Ｓｉｒｉｕｓ Ｒｅｄ Ｆ３ＢＡ（Ｄｉｒｅｃｔ ｒｅｄ ８０：ＳＩＧＭＡ社）／０．５Ｍ 酢酸水溶液））を各ウェルに５０μｌ滴下し、室温で１時間インキュベーションした。その後、各ウェルの細胞を１０ｍＭ 塩酸で３回洗浄し、０．１Ｍ 水酸化ナトリウム水溶液を各ウェルに１００μｌ添加し、室温で５分間インキュベーションして色素を抽出して、被験反応液および対照ブランク液を得た。
（４）吸光度の測定およびコラーゲン産生促進活性の算出
上記の工程（３）のインキュベーション終了後、プレートを軽く撹拌し、マイクロプレートリーダー（モデル６８０：バイオラッド社製）を用いて、プレート中の被験反応液および対照ブランク液の５４０ｎｍの吸光度を測定した。なお、測定される吸光度は、コラーゲンに特異的結合したシリウスレッドに由来する。
下記の式を用いて、得られた各測定値からコラーゲン産生促進活性を算出した。なお、該活性は、試料溶液を添加していない対照ブランクの細胞のコラーゲン産生活性を１００％とした場合の相対的な数値として算出される。
コラーゲン産生促進活性（％）＝（Ｓ／Ｃ）×１００
（Ｓ：被験反応液の測定値、Ｃ：対照ブランク液の測定値）
得られた結果を表１に示す。
表１より、ボダイジュ花、シャクヤク花およびテルミナリア アルジュナの各抽出物は、正常ヒト新生児***線維芽細胞ＮＨＤＦ（ＮＢ）に対してコラーゲン産生促進活性を示すことが確認された。
(3) Preparation of test reaction solution and control blank solution After culturing in the above step (2), the medium was removed from each well and the cells were washed with PBS (-), and then Sirius red reagent (0.1% Sirius 50 μl of Red F3BA (Direct red 80: SIGMA) /0.5 M acetic acid aqueous solution) was dropped into each well and incubated at room temperature for 1 hour. Thereafter, the cells in each well were washed 3 times with 10 mM hydrochloric acid, 100 μl of 0.1 M aqueous sodium hydroxide solution was added to each well, and the dye was extracted by incubation at room temperature for 5 minutes. Got.
(4) Measurement of absorbance and calculation of collagen production promoting activity After the completion of the incubation in the above step (3), the plate was gently stirred and tested in the plate using a microplate reader (Model 680: manufactured by Bio-Rad). The absorbance at 540 nm of the reaction solution and the control blank solution was measured. The absorbance measured is derived from sirius red that is specifically bound to collagen.
Collagen production promoting activity was calculated from the obtained measured values using the following formula. The activity is calculated as a relative numerical value when the collagen production activity of the control blank cells to which no sample solution is added is 100%.
Collagen production promoting activity (%) = (S / C) × 100
(S: measured value of test reaction solution, C: measured value of control blank solution)
The obtained results are shown in Table 1.
From Table 1, it was confirmed that each extract of Bodaiju flower, peony flower and Terminaria arjuna exhibits collagen production promoting activity against normal human neonatal foreskin fibroblasts NHDF (NB).
試験例２：ヒアルロニダーゼ阻害活性試験
ボダイジュ花、シャクヤク花およびテルミナリア アルジュナの各抽出物のヒアルロニダーゼ阻害活性を、ヒアルロン酸などの糖類の定量法であるモルガン−エルソン法（生化学実験，第１１巻，第２２−２３頁（１９６８年）参照）を応用して評価した。
（１）試料溶液の調製
ボダイジュ花抽出物、シャクヤク花抽出物およびテルミナリア アルジュナ抽出物を、ジメチルスルホキシド（ＤＭＳＯ）に溶解し、各試料溶液（１２．５ｍｇ/ｍｌ）を調製した。
（２）酵素溶液の調製
牛精巣ヒアルロニダーゼ（ＳＩＧＭＡ社）を０．１M酢酸緩衝液（ｐＨ４．０）に溶解し、酵素溶液（４００ユニット／ｍｌ）を調製した。
（３）酵素活性化溶液の調製
ｃｏｍｐｏｕｎｄ４８／８０（ＳＩＧＭＡ社）を０．１M酢酸緩衝液（ｐＨ４．０）に溶解し、酵素活性化溶液（０．１ｍｇ／ｍｌ）を調製した。
Test Example 2: Hyaluronidase Inhibitory Activity Test The hyaluronidase inhibitory activity of each extract of bodaiju flower, peony flower and terminaria arjuna was determined using the Morgan-Elson method (Biochemical Experiments, Vol. 11, Vol. 11). 22-23 (1968)) was applied for evaluation.
(1) Preparation of sample solution Bodaiju flower extract, peony flower extract and Terminaria arjuna extract were dissolved in dimethyl sulfoxide (DMSO) to prepare each sample solution (12.5 mg / ml).
(2) Preparation of enzyme solution Cattle testicular hyaluronidase (SIGMA) was dissolved in 0.1 M acetic acid buffer (pH 4.0) to prepare an enzyme solution (400 units / ml).
(3) Preparation of enzyme activation solution Compound 48/80 (SIGMA) was dissolved in 0.1 M acetate buffer (pH 4.0) to prepare an enzyme activation solution (0.1 mg / ml).
（４）基質溶液の調製
ヒアルロン酸カリウム（和光純薬社）を０．１M酢酸緩衝液（ｐＨ４．０）に溶解し、基質溶液（０．４ｍｇ／ｍｌ）を調製した。
（５）ホウ酸溶液の調製
ホウ酸（和光純薬社）４．９５ｇに水５０ｍｌを加え、１Ｎ 水酸化ナトリウム溶液でｐＨ９．１にした後、水を加えて１００ｍｌに調製した。
（６）ｐ−ジメチルアミノベンズアルデヒド（ｐ−ＤＡＢ）試薬の調製
ｐ−ＤＡＢ（和光純薬社）１０ｇを１０Ｎ 塩酸１２．５ｍｌと酢酸８７．５ｍｌとの混液に溶解し、ｐ−ＤＡＢ試薬（０．１ｇ／ｍｌ）を調製した。なお、該試薬は使用時まで冷蔵保存し、使用直前に酢酸で１０倍容量に希釈して、濃度を１０ｍｇ／ｍｌとした。
(4) Preparation of substrate solution Potassium hyaluronate (Wako Pure Chemical Industries, Ltd.) was dissolved in 0.1 M acetic acid buffer (pH 4.0) to prepare a substrate solution (0.4 mg / ml).
(5) Preparation of boric acid solution 50 ml of water was added to 4.95 g of boric acid (Wako Pure Chemical Industries, Ltd.), adjusted to pH 9.1 with 1N sodium hydroxide solution, and then adjusted to 100 ml with water.
(6) Preparation of p-dimethylaminobenzaldehyde (p-DAB) reagent 10 g of p-DAB (Wako Pure Chemical Industries, Ltd.) was dissolved in a mixed solution of 12.5 ml of 10N hydrochloric acid and 87.5 ml of acetic acid, and p-DAB reagent (0 0.1 g / ml). The reagent was stored refrigerated until use, and diluted 10-fold with acetic acid immediately before use to give a concentration of 10 mg / ml.
（７）被験反応液および被験ブランク液の調製
９６ディープウェルプレート（マイクロプレート：ステム社製）に各試料溶液を２μｌ／ウェルで添加し、次いでこれらのウェルに酵素溶液２８μｌを加えて、３７℃で２０分間インキュベーションした。また、被験ブランクとして、酵素溶液に代えて０．１M酢酸緩衝液（ｐＨ４．０）２８μｌを被験ブランク用のウェルに添加した。
次いで、酵素活性化溶液２０μｌを各ウェルに加えて３７℃で２０分間インキュベーションした。さらに、基質溶液５０μｌを加えて３７℃で４０分間反応させた。その後、０．４Ｎ 水酸化ナトリウム水溶液２０μｌを加え、プレートを氷冷して反応を停止させた。そして、ホウ酸溶液２０μｌを加え、プレートを沸騰浴にて１００〜１２０℃で５分間加熱した後、氷冷した。その後、各ウェルにｐ−ＤＡＢ試薬３００μｌを加え、プレートを３７℃で２０分間インキュベーションして発色させて、被験反応液および被験ブランク液を得た。
（８）対照反応液および対照ブランク液の調製
上記の工程（７）において、各試料溶液に代えてＤＭＳＯを９６ディープウェルプレートに２μｌ／ウェルで添加し、以後の操作を上記の工程（７）と同様にして、対照反応液および対照ブランク液を得た。
(7) Preparation of test reaction solution and test blank solution Each sample solution was added to a 96 deep well plate (Microplate: manufactured by Stem) at 2 μl / well, and then 28 μl of enzyme solution was added to these wells at 37 ° C. For 20 minutes. As a test blank, 28 μl of 0.1 M acetate buffer (pH 4.0) was added to the well for the test blank instead of the enzyme solution.
Then 20 μl of enzyme activation solution was added to each well and incubated at 37 ° C. for 20 minutes. Further, 50 μl of the substrate solution was added and reacted at 37 ° C. for 40 minutes. Thereafter, 20 μl of 0.4N aqueous sodium hydroxide solution was added, and the reaction was stopped by cooling the plate with ice. Then, 20 μl of boric acid solution was added, and the plate was heated in a boiling bath at 100 to 120 ° C. for 5 minutes, and then cooled on ice. Thereafter, 300 μl of p-DAB reagent was added to each well, and the plate was incubated at 37 ° C. for 20 minutes to develop a color to obtain a test reaction solution and a test blank solution.
(8) Preparation of control reaction solution and control blank solution In the above step (7), DMSO was added to the 96 deep well plate at 2 μl / well instead of each sample solution, and the subsequent operation was performed in the above step (7). In the same manner as described above, a control reaction solution and a control blank solution were obtained.
（９）吸光度の測定およびヒアルロニダーゼ阻害活性の算出
上記の工程（７）および（８）のインキュベーション終了後、マイクロプレートリーダー（モデル６８０：バイオラッド社製）を用いて、プレート中の被験反応液、被験ブランク液、対照反応液および対照ブランク液の５８５ｎｍにおける吸光度を測定した。
そして、次の式を用いて、得られた各測定値からヒアルロニダーゼ阻害活性を算出した。
ヒアルロニダーゼ阻害活性（％）＝｛１−（Ｓ−Ｓb）／（Ｃ−Ｃb）｝×１００
（Ｓ：被験反応液の測定値、Ｓb：被験ブランク液の測定値、Ｃ：対照反応液の測定値、
Ｃb：対照ブランク液の測定値）
得られた結果を表２に示す。
表２より、ボダイジュ花、シャクヤク花およびテルミナリア アルジュナの各抽出物がヒアルロニダーゼ阻害活性を有することが確認された。
(9) Measurement of absorbance and calculation of hyaluronidase inhibitory activity After completion of the incubation in the above steps (7) and (8), using a microplate reader (Model 680: manufactured by Bio-Rad), the test reaction solution in the plate, The absorbance at 585 nm of the test blank solution, the control reaction solution, and the control blank solution was measured.
And the hyaluronidase inhibitory activity was computed from each obtained measured value using the following formula | equation.
Hyaluronidase inhibitory activity (%) = {1− (S−S b ) / (C−C b )} × 100
(S: measured value of test reaction solution, S b : measured value of test blank solution, C: measured value of control reaction solution,
Cb : measured value of control blank solution)
The obtained results are shown in Table 2.
From Table 2, it was confirmed that each extract of Bodaiju flower, peony flower and Terminaria arjuna has hyaluronidase inhibitory activity.
試験例３：スーパーオキサイド消去活性（ＳＯＤ様活性）試験
ボダイジュ花、シャクヤク花およびテルミナリア アルジュナの各抽出物のスーパーオキサイド消去活性を、以下のようにＳＯＤ Ａｓｓａｙ Ｋｉｔ−ＷＳＴ（株式会社同仁化学研究所）を用いて評価した。
上記のキットは、キサンチンとキサンチンオキシダーゼとの反応によってスーパーオキサイドを発生させ、さらに、スーパーオキサイドと該キットの発色試薬との反応によって黄色のテトラゾリウム塩ＷＳＴ−１が生成することを利用するものである。例えば、試験対象の試料がスーパーオキサイド消去活性を有する場合、発生したスーパーオキサイドが消去されるため、ＷＳＴ−１の生成量が減少し、反応液の黄色の発色が抑制される。したがって、該反応液の吸光度を測定することにより、スーパーオキサイド消去活性をＷＳＴ−１の生成抑制率として求めることができる。
Test Example 3: Superoxide scavenging activity (SOD-like activity) test The superoxide scavenging activity of each extract of bodaiju flower, peony flower and terminaria arjuna was determined as follows. SOD Assay Kit-WST (Dojindo Laboratories, Inc.) Was used to evaluate.
The above kit utilizes the fact that superoxide is generated by the reaction of xanthine and xanthine oxidase, and the yellow tetrazolium salt WST-1 is generated by the reaction of the superoxide and the coloring reagent of the kit. . For example, when the sample to be tested has superoxide scavenging activity, the generated superoxide is erased, so the amount of WST-1 produced is reduced, and yellow color development of the reaction solution is suppressed. Therefore, by measuring the absorbance of the reaction solution, the superoxide scavenging activity can be determined as the WST-1 production inhibition rate.
（１）試験溶液の調製
ボダイジュ花抽出物、シャクヤク花抽出物及びテルミナリア アルジュナ抽出物を、５０％（ｖ／ｖ）エタノールに溶解し、各試料溶液（１２０μｇ／ｍｌ）を調製した。
（２）被験反応液および被験ブランク液の調製
９６ウェルプレート（マイクロプレート：アズワン株式会社製）に各試料溶液を２０μｌ／ウェルで添加し、次いでこれらのウェルにＷＳＴ溶液を２００μｌ加え、プレートミキサーで撹拌した。その後、各ウェルに酵素溶液を２０μｌ加えた。また、ブランク対照として、酵素溶液に代えて希釈緩衝液を２０μｌ加えた。その後、プレートを３７℃で２０分間インキュベートし、被験反応液および被験ブランク液を得た。
(1) Preparation of test solution Bodaiju flower extract, peony flower extract and Terminaria arjuna extract were dissolved in 50% (v / v) ethanol to prepare each sample solution (120 μg / ml).
(2) Preparation of test reaction solution and test blank solution Each sample solution was added to a 96-well plate (Microplate: manufactured by ASONE Co., Ltd.) at 20 μl / well, and then 200 μl of WST solution was added to these wells. Stir. Thereafter, 20 μl of enzyme solution was added to each well. As a blank control, 20 μl of dilution buffer was added instead of the enzyme solution. Thereafter, the plate was incubated at 37 ° C. for 20 minutes to obtain a test reaction solution and a test blank solution.
（３）対照反応液および対照ブランク液の調製
上記の工程（２）において、各試料溶液に代えて５０％（ｖ／ｖ）エタノールを９６ウェルプレートに２０μｌ／ウェルで添加し、以後の操作を上記の工程（２）と同様にして、対照反応液および対照ブランク液を得た。
（４）吸光度の測定およびスーパーオキサイド消去活性の算出
上記の工程（２）および（３）のインキュベーション終了後、マイクロプレートリーダー（モデル６８０：バイオラッド社製）を用いて、プレート中の被験反応液、被験ブランク液、対照反応液および対照ブランク液の４５０ｎｍにおける吸光度を測定した。
そして、次の式を用いて、得られた各測定値からスーパーオキサイド消去活性を算出した。
スーパーオキサイド消去活性（％）＝｛１−（Ｓ−Ｓb）／（Ｃ−Ｃb）｝×１００
（Ｓ：被験反応液の測定値、Ｓb：被験ブランク液の測定値、Ｃ：対照反応液の測定値、
Ｃb：対照ブランク液の測定値）
得られた結果を表３に示す。
表３より、ボダイジュ花、シャクヤク花およびテルミナリア アルジュナの各抽出物がスーパーオキサイド消去活性を有することが確認された。
(3) Preparation of control reaction solution and control blank solution In the above step (2), instead of each sample solution, 50% (v / v) ethanol was added to a 96-well plate at 20 μl / well, and the subsequent operation was performed. A control reaction solution and a control blank solution were obtained in the same manner as in the above step (2).
(4) Measurement of absorbance and calculation of superoxide scavenging activity After completion of the incubation in the above steps (2) and (3), using a microplate reader (model 680: manufactured by Bio-Rad), a test reaction solution in the plate The absorbance at 450 nm of the test blank solution, the control reaction solution, and the control blank solution was measured.
And superoxide elimination activity was computed from each obtained measured value using the following formula.
Superoxide elimination activity (%) = {1− (S−S b ) / (C−C b )} × 100
(S: measured value of test reaction solution, S b : measured value of test blank solution, C: measured value of control reaction solution,
Cb : measured value of control blank solution)
The obtained results are shown in Table 3.
From Table 3, it was confirmed that each extract of Bodaiju flower, peony flower and Terminaria arjuna has superoxide scavenging activity.
試験例４：ＤＰＰＨラジカル消去活性試験
ボダイジュ花、シャクヤク花およびテルミナリア アルジュナの各抽出物のＤＰＰＨラジカル消去活性を、ＤＰＰＨラジカルの減少量を測定することにより評価した。なお、この試験においては、ラジカル消去活性を有する標準物質としてビタミンＥ誘導体Ｔｒｏｌｏｘ（商標）を用いた。
（１）試料溶液の調製
ボダイジュ花抽出物、シャクヤク花抽出物およびテルミナリア アルジュナ抽出物を、ＤＭＳＯに溶解し、各試料溶液（２ｍｇ/ｍｌ）を調製した。
（２）ＤＰＰＨ／エタノール溶液の調製
ＤＰＰＨ（和光純薬株式会社）をエタノールに溶解し、ＤＰＰＨ／エタノール溶液（０．４ｍＭ）を調製した。なお、試験には調製から２時間以内のものを使用した。
（３）ＭＥＳ緩衝液の調製
ＭＥＳ緩衝液（株式会社同仁化学研究所）を水で希釈し、１Ｎ 水酸化ナトリウム水溶液でｐＨ６．１に調整後、２００ｍＭ ＭＥＳ緩衝液とした。
（４）ＤＰＰＨ希釈標準溶液の調製
０．４ｍＭ ＤＰＰＨ／エタノール溶液、２００ｍＭ ＭＥＳ緩衝液および水を２：１：１の割合で混合し、ＤＰＰＨ希釈標準溶液を調製した。
（５）被験反応液および対照ブランク液の調製
９６ウェルプレート（マイクロプレート：アズワン株式会社製）に各試料溶液を１０μｌ／ウェルで添加し、次いでこれらのウェルにＤＰＰＨ希釈標準溶液１９０μlを加えた。また、被験ブランクとして試料溶液に代えて、ＤＭＳＯを各ウェルに１０μｌ添加した。その後、プレートを室温で３０分間インキュベートして、被験反応液および対照ブランク液を得た。
（６）対照反応液の調製
上記の工程（５）において、各試料溶液に代えて２０ｍＭ Ｔｒｏｌｏｘ（商標）（和光純薬工業株式会社）を９６ウェルプレートの各ウェルに２０μｌ添加し、以後の操作を上記の工程（５）と同様にして、対照反応液を得た。
そして、次の式を用いて、対照反応液のＤＰＰＨラジカル消去活性を１００％として各試料溶液の該活性を算出した。
ＤＰＰＨラジカル消去活性（％）＝｛（Ｃb−Ｓ）／（Ｃb−Ｃ）｝×１００
（Ｓ：被験反応液の測定値、Ｃb：対照ブランク液の測定値、Ｃ：対照反応液の測定値）
得られた結果を表４に示す。
表４より、ボダイジュ花、シャクヤク花およびテルミナリア アルジュナの各抽出物がＤＰＰＨラジカル消去活性を有することが確認された。
Test Example 4: DPPH Radical Scavenging Activity Test DPPH radical scavenging activity of each extract of bodaiju flower, peony flower and terminaria arjuna was evaluated by measuring the decrease in DPPH radical. In this test, a vitamin E derivative Trolox (trademark) was used as a standard substance having radical scavenging activity.
(1) Preparation of sample solution Bodaiju flower extract, peony flower extract and Terminaria arjuna extract were dissolved in DMSO to prepare each sample solution (2 mg / ml).
(2) Preparation of DPPH / ethanol solution DPPH (Wako Pure Chemical Industries, Ltd.) was dissolved in ethanol to prepare a DPPH / ethanol solution (0.4 mM). The test was performed within 2 hours after preparation.
(3) Preparation of MES buffer MES buffer (Dojindo Laboratories, Inc.) was diluted with water, adjusted to pH 6.1 with 1N aqueous sodium hydroxide solution, and then used as 200 mM MES buffer.
(4) Preparation of DPPH diluted standard solution 0.4 mM DPPH / ethanol solution, 200 mM MES buffer and water were mixed at a ratio of 2: 1: 1 to prepare a DPPH diluted standard solution.
(5) Preparation of test reaction solution and control blank solution Each sample solution was added at 10 μl / well to a 96-well plate (Microplate: manufactured by ASONE Co., Ltd.), and then 190 μl of DPPH diluted standard solution was added to these wells. In addition, 10 μl of DMSO was added to each well instead of the sample solution as a test blank. Thereafter, the plate was incubated at room temperature for 30 minutes to obtain a test reaction solution and a control blank solution.
(6) Preparation of Control Reaction Solution In the above step (5), 20 μl of 20 mM Trolox ™ (Wako Pure Chemical Industries, Ltd.) was added to each well of a 96-well plate instead of each sample solution, and the subsequent operations In the same manner as in the above step (5), a control reaction solution was obtained.
Then, using the following formula, the activity of each sample solution was calculated with the DPPH radical scavenging activity of the control reaction solution as 100%.
DPPH radical scavenging activity (%) = {(C b -S) / (C b -C)} × 100
(S: measured value of test reaction solution, C b : measured value of control blank solution, C: measured value of control reaction solution)
Table 4 shows the obtained results.
From Table 4, it was confirmed that each extract of Bodaiju flower, Peonies flower and Terminaria arjuna has DPPH radical scavenging activity.
以下に、本発明の美容用組成物の処方例を示す。なお、各抽出物は、製造例１の方法によって得られたものを用いた。
実施例１〜７
クリーム
本発明の美容用組成物（皮膚外用剤）である、化粧品（クリーム）を製造した。各実施例における各成分の配合割合を表５に重量％で示す。
（Ａ）の各成分を混合し、８０℃に加熱した。一方、（Ｂ）の各成分をそれぞれ混合し８０℃に加熱した。（Ａ）の混合物に、（Ｂ）の混合物を撹拌しながら徐々に加えて乳化させ、その後３５℃に冷却して、クリームを得た。
Below, the formulation example of the cosmetic composition of this invention is shown. In addition, what was obtained by the method of manufacture example 1 was used for each extract.
Examples 1-7
Cream A cosmetic (cream), which is the cosmetic composition (external preparation for skin) of the present invention, was produced. The blending ratio of each component in each example is shown in Table 5 in wt%.
Each component of (A) was mixed and heated to 80 ° C. On the other hand, the components (B) were mixed and heated to 80 ° C. To the mixture of (A), the mixture of (B) was gradually added and emulsified with stirring, and then cooled to 35 ° C. to obtain a cream.
実施例８〜１４
化粧水
本発明の美容用組成物（皮膚外用剤）である、化粧品（化粧水）を製造した。各実施例における各成分の配合割合を表６に重量％で示す。
（Ａ）の各成分を混合し、８０℃に加熱して、さらに混合、均一化した後、冷却する。これに３５℃で（Ｂ）の各成分を順次添加し、混合、均一化して、ローションを得た。
Examples 8-14
Cosmetic lotion A cosmetic product (skin lotion) which is the cosmetic composition (external preparation for skin) of the present invention was produced. The blending ratio of each component in each example is shown in Table 6 in wt%.
The components (A) are mixed, heated to 80 ° C., further mixed and homogenized, and then cooled. To this, each component of (B) was sequentially added at 35 ° C., mixed and homogenized to obtain a lotion.
実施例１５〜２１
錠剤
本発明の美容用組成物である、錠剤の形態にある食品を製造した。各実施例における各成分の配合割合を表７に重量％で示す。
各成分を混合、均一化した後、これを打錠機により形成し、錠剤の形態にある食品を得た。
Examples 15-21
Tablet A food product in the form of a tablet, which is the cosmetic composition of the present invention, was produced. The blending ratio of each component in each example is shown in Table 7 in wt%.
After each component was mixed and homogenized, it was formed with a tableting machine to obtain a food in the form of tablets.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010091615A JP5921056B2 (en) | 2009-07-22 | 2010-04-12 | Cosmetic composition |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009171143 | 2009-07-22 | ||
| JP2009171143 | 2009-07-22 | ||
| JP2010091615A JP5921056B2 (en) | 2009-07-22 | 2010-04-12 | Cosmetic composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2011042644A true JP2011042644A (en) | 2011-03-03 |
| JP5921056B2 JP5921056B2 (en) | 2016-05-24 |
Family
ID=43830343
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010091615A Active JP5921056B2 (en) | 2009-07-22 | 2010-04-12 | Cosmetic composition |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5921056B2 (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012087112A (en) * | 2010-09-21 | 2012-05-10 | Kyoei Kagaku Kogyo Kk | Cosmetic |
| JP2013107859A (en) * | 2011-11-24 | 2013-06-06 | Nihon Kolmar Co Ltd | Skin cosmetic preparation for external use comprising connarus extract |
| JP2013203734A (en) * | 2012-03-29 | 2013-10-07 | Nagase Chemtex Corp | Photoaging inhibitor |
| JP5888574B1 (en) * | 2015-07-02 | 2016-03-22 | 株式会社東洋新薬 | Blood flow improver |
| JP2016135811A (en) * | 2016-04-26 | 2016-07-28 | 日本コルマー株式会社 | Skin cosmetics for external use containing connarus ruber extract |
| JP2019059700A (en) * | 2017-09-28 | 2019-04-18 | ポーラ化成工業株式会社 | Labial hyaluronic acid production promoter |
| JP2020128382A (en) * | 2015-05-18 | 2020-08-27 | 共栄化学工業株式会社 | Vitamin d-like active agent |
-
2010
- 2010-04-12 JP JP2010091615A patent/JP5921056B2/en active Active
Non-Patent Citations (5)
| Title |
|---|
| JPN6014003518; Ｆｏｏｄ Ｍｉｃｒｏｂｉｏｌｏｇｙ ４（１）, 1987, ９７-１００ * |
| JPN6014003519; フレグランスジャーナル臨時増刊 Ｎｏ．６, 1986, 第１９６， ３２９， ３３０， ３３７， ３４０頁 * |
| JPN6014003521; フレグランスジャーナル ３２（１２）, 2004, ５１-５５ * |
| JPN6014003522; 世界有用植物事典 , 1989, 第１０４２， １０４３頁 * |
| JPN6014003523; フレグランスジャーナル ２４（１２）, 1996, ５３-５８ * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012087112A (en) * | 2010-09-21 | 2012-05-10 | Kyoei Kagaku Kogyo Kk | Cosmetic |
| JP2013107859A (en) * | 2011-11-24 | 2013-06-06 | Nihon Kolmar Co Ltd | Skin cosmetic preparation for external use comprising connarus extract |
| JP2013203734A (en) * | 2012-03-29 | 2013-10-07 | Nagase Chemtex Corp | Photoaging inhibitor |
| JP2020128382A (en) * | 2015-05-18 | 2020-08-27 | 共栄化学工業株式会社 | Vitamin d-like active agent |
| JP7033160B2 (en) | 2015-05-18 | 2022-03-09 | 共栄化学工業株式会社 | Vitamin D-like agent |
| JP5888574B1 (en) * | 2015-07-02 | 2016-03-22 | 株式会社東洋新薬 | Blood flow improver |
| JP2017014165A (en) * | 2015-07-02 | 2017-01-19 | 株式会社東洋新薬 | Blood flow improvement agent |
| JP2016135811A (en) * | 2016-04-26 | 2016-07-28 | 日本コルマー株式会社 | Skin cosmetics for external use containing connarus ruber extract |
| JP2019059700A (en) * | 2017-09-28 | 2019-04-18 | ポーラ化成工業株式会社 | Labial hyaluronic acid production promoter |
| JP7039812B2 (en) | 2017-09-28 | 2022-03-23 | ポーラ化成工業株式会社 | Hyaluronic acid production promoter for lips |
Also Published As
| Publication number | Publication date |
|---|---|
| JP5921056B2 (en) | 2016-05-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5850929B2 (en) | Composition for improving the appearance of the skin by stimulating MAGP-1 | |
| JP5921056B2 (en) | Cosmetic composition | |
| KR20200026646A (en) | Cosmetic Composition for Improving Skin Condition Comprising Plant cell complex cultures to improve skin radiance and vitality | |
| KR101387308B1 (en) | Skin whitening composition by using of dendropanax morbifera ferment extract | |
| JP4931356B2 (en) | Glutamate decarboxylase activator | |
| TW201206494A (en) | Use of Tiliacora triandra in cosmetics and compositions thereof | |
| CN113694115A (en) | Application of Fuzhuan tea extract in preparation of skin conditioning product | |
| KR20230147028A (en) | Composition for anti-aging, anti-inflammation or skin regeneration containing Cannabis sativa stem extract as effective component | |
| KR101313086B1 (en) | Cosmetic composition having anti-aging effect | |
| KR20100014100A (en) | Cosmetic composition containing fermented extracts with salt of natural materials | |
| KR101528275B1 (en) | Cosmetic composition with the extract of Solidago virgaurea L. var coreana Nakai, Aster tataricus, Saxifraga stolonifera | |
| JP2006124356A (en) | INTERLEUKIN-1alpha PRODUCTION INHIBITOR | |
| CN109528536A (en) | A kind of skin care item and preparation method thereof containing choline ellagic acid salt | |
| KR20140145278A (en) | Cosmetic composition containing Cimicifuga heracleifolia extract, Cornus officinalis extract and Geranium nepalense extract for skin convergence and elasticity effect | |
| KR101489935B1 (en) | Method for preparing mugwort extract and cosmetic composition comprising mugwort extract prepared therefrom | |
| JP2023171950A (en) | Anti-aging agent, antioxidant, anti-inflammatory agent, and whitening agent, as well as cosmetic | |
| KR102029546B1 (en) | Anti-aging and anti-allergic cosmetic compositon comprising the extract of the flower, root, callus and seed of Hibiscus syriacus L. as an active ingredient and preparation method of the same | |
| JP2006182731A (en) | Cosmetic composition or food and drink | |
| JP2011020990A (en) | Magnolia obovata extract, method for producing the same, and skin-beautifying composition containing the same | |
| KR101550275B1 (en) | Method for making glue from soft-shelled turtle and cosmetics containing the same | |
| JP2004191106A (en) | Screening method of hair cultivating agent effective component and its utilization | |
| KR20180082058A (en) | A cosmetic composition for skin desquamation comprising natural complex extract | |
| JP5134759B2 (en) | Degreasing inhibitor and topical skin preparation containing degranulating inhibitor | |
| JP7233832B2 (en) | hair cosmetics | |
| KR20140082276A (en) | A cosmetic composition comprising extracts of Rhynchosia nulubilis processed with Glycyrrhiza uralensis for whitening skin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20130129 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140204 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140312 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20140805 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20141104 |
|
| A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20141111 |
|
| A912 | Re-examination (zenchi) completed and case transferred to appeal board |
Free format text: JAPANESE INTERMEDIATE CODE: A912 Effective date: 20150109 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20160412 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 5921056 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |