CN102701823B - Production method of selenium-rich high-quality honeysuckle - Google Patents
Production method of selenium-rich high-quality honeysuckle Download PDFInfo
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- CN102701823B CN102701823B CN201210187141.XA CN201210187141A CN102701823B CN 102701823 B CN102701823 B CN 102701823B CN 201210187141 A CN201210187141 A CN 201210187141A CN 102701823 B CN102701823 B CN 102701823B
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Abstract
The invention belongs to the technical field of plant cultivation, and provides a production method of selenium-rich high-quality honeysuckle. The production method comprises the following steps of: mixing farmyard manure and sodium selenite, and applying the mixture onto a root part of the honeysuckle when in budding dates of the honeysuckle; adding rare earth micronutrients, sodium selenite, sodium hydrogen sulfite and surface active agent into water, stirring and dissolving, and spraying the dissolved mixture onto leaf surfaces when in honeysuckle flourishing period and bud stage; and airing the collected honeysuckle to obtain a honeysuckle product. Due to a rear earth-amino acid binary coordination compound, the function of a plant growth regulator is achieved; the content of heavy metal ion is reduced; the contents of effective component chlorogenic acid and galuteolin are improved; the yield and the quality of the honeysuckle are improved further; and the sodium hydrogen sulfite provides an acid environment for the honeysuckle, so that the absorption on selenium elements is promoted. The production method of the selenium-rich high-quality honeysuckle adopts a biotransformation method so as to transform inorganic selenium to organic selenium; solves the problem that the honeysuckle produced by a traditional planting method only contains basic effective components, so as not to reach the aim of supplement of selenium after being eaten; and can be used for batch production of the honeysuckle.
Description
Technical field
The invention belongs to plant cultivation technical field, relate in particular to the production method of a kind of rich selenium high-quality honeysuckle.
Background technology
Selenium is the essential micronutrient elements of animals and humans.The international selenium recommendation daily intaking amount 60-400 μ g of association, daily intaking amount 50-200 μ g recommends in Chinese Soclety of Nutrition.And according to China's nutrition survey, the only 26-32 μ g of selenium intake being grown up every day.Therefore, population of China selemium nutrition is bad is universal phenomenon, and China lacks selenium soil and accounts for the whole nation 72%, lacks selenium population and reaches more than 100,000,000.
Science is verified now, selenium has the effect of 400 various diseases such as anti-cancer, anticancer, control cardiovascular and cerebrovascular disease, hepatopathy, diabetes, gynaecological disease, prostate and cataract, have anti-oxidant, remove interior free yl improve immunity, many specific functions delay senility, go down and aging course etc. as cardiovascular and cerebrovascular disease, cancer, diabetes, body immunity, all relevant with scarce selenium.And in the day by day serious situation of environmental pollution, selenium has the special physiological functions such as the heavy metal poisoning of releasing.Therefore, human body is mended selenium and is not only the requirement that improves immunity, is also the effective way that strengthens the resistance to environmental poisonous substance; Selenium in natural food can not meet the normal need of human body, and economic, healthy, safe selenium-enriched food is the effective way that the mankind mend selenium.Animal is mended sodium selenite for selenium and adds and food in the past, but its biological effectiveness is low, and takes in narrow range, easily produces excess intake and poisoning problem, and rich by reference selenium Lonicera flower tea can be when leisure be had tea easy to do Selenium Supplement element.
Studies confirm that, rare earth element does not belong to the nutritive element of plant, it is mainly that rare earth belongs to physiologically active of plant material that Rational Application rare earth impels the reason of increasing crop yield and high-quality, there is the function of regulating plant body physiological activity, can promote the absorption of crops to nutrients such as nitrogen, phosphorus, potassium, increase the chloroplast content of plant, improve intensity of photosynthesis, promote enzymic activity, promote crop floral organ to grow, early flowering, suitable rare earth concentration can promote seed germination to promote seed germination and root growth, reduce the crop incidence of disease, strengthen the anti-adversity ability of crop.Rare earth is residual little, is a kind of important plant growth regulator being worthy to be popularized.Therefore, someone thinks that rare earth application has been most important application and development since rare earth is found 200 years.
In recent years, along with going deep into of rare earth application theory and practice, people start rare earth element for Chinese herbal medicine, to improving Chinese herbal medicine quantity and quality, improve the content of Chinese herbal medicine effective ingredients undoubtedly, finally improve curative effect of medication, have opened up new way.Research shows to use rare earth on Chinese herbal medicine, can promote root growth, stratification, reduce disease, improve emergence rate, and accelerate seedling and grow tall and increase slightly, improve chlorophyll content, promotion nitrogen, phosphorus, potassium absorption, improve soluble sugar content and amino acid whose content, improve quality, strengthen plant resistance.Use the trophic level that rare earth can obviously improve Chinese herbal medicine, promote growing of plant, increase dry-matter accumulation and chlorophyll content, obviously reduce fruit drop.
Summary of the invention
The invention provides the production method of a kind of rich selenium high-quality honeysuckle, be intended to solve in the honeysuckle of prior art production, content and the validity of trace elements of selenium are lower, and effective component chlorogenic acid and galuteolin content are lower, the problem that the safety of honeysuckle and economy are poor.
The production method that the object of the present invention is to provide a kind of rich selenium high-quality honeysuckle, this production method comprises the following steps:
Step 1, at the sprouting period of honeysuckle, will impose on honeysuckle root after farmyard manure and sodium selenite mixing;
Step 2, RE microbial fertilizer, sodium selenite, sodium hydrogensulfite and the surfactant stirring and dissolving that is added to the water, carries out liquid level sprinkling by the mixed liquor of dissolving for a long time honeysuckle is prosperous;
Step 3, in the rice flower bud phase of honeysuckle, is sprayed on honeysuckle with the mixed liquor of preparing in step 2;
Step 4, dries the honeysuckle after gathering, and obtains honeysuckle finished product.
Further, the weight ratio of farmyard manure and sodium selenite is, farmyard manure: sodium selenite=100: 0.001-0.2; Fertilising consumption is the every pier of 2-3kg/.
Further, with RE microbial fertilizer, sodium selenite, sodium hydrogensulfite and the surfactant stirring and dissolving that is added to the water, the implementation method that obtains mixed liquor is:
Be uniformly mixed with 10 parts of weight ratio RE microbial fertilizer, sodium selenite 0.01-0.04 part, sodium hydrogensulfite 0.05-2 part;
It is that 20-50 DEG C, weight ratio are in the water of 100 parts that fertilizer after being uniformly mixed is joined to temperature, and adds surfactant stirring and dissolving, obtains mixed liquor.
Further, RE microbial fertilizer main component is nitrogen, phosphorus, potassium and rare earth-amino acid two-element match, the N in RE microbial fertilizer: P
2o
5: K
2o: the weight ratio of rare earth-amino acid two-element match is 20: 10: 10: 0.001-0.05.
Further, the general formula of the rare earth-amino acid two-element match in RE microbial fertilizer is REx-(AA) y, wherein x=1-2, y=1-4, rare earth RE in formula is any one trivalent ion in La, Ce, Pm, Nd, (AA) in formula is amino acid, is selected from lysine, threonine, glycine, leucine, isoleucine, valine, methionine, tyrosine, tryptophan, phenyl alanine.
Further, at the sprouting period of honeysuckle, by the implementation method that imposes on honeysuckle root after farmyard manure and sodium selenite mixing be: the mixture of farmyard manure and sodium selenite is executed and is embedded in topsoil 20cm, spread thin soil above and cover.
Further, in rice flower bud phase of honeysuckle while spraying the mixed liquor being formed by RE microbial fertilizer, sodium selenite, sodium hydrogensulfite and surfactant, after should being chosen in cloudy day or fine day at 4 o'clock in afternoon, spray in latter 24 hours, in case of rainy, fill spray 1 time.
Further, when the rare earth-amino acid two-element match in RE microbial fertilizer is La (Gly)
4cl
33H
2when O, rare earth-amino acid two-element match La (Gly)
4cl
33H
2the synthetic method of O is:
Take a certain amount of La
2o
3solid, is dissolved in HCl, allow it fully react and in water-bath slow evaporation to dry;
With acetone washing, be placed on and in NaOH drier, be dried to constant weight and make LaCl
3hydrate;
Accurately take 1mmoL LaCl
3hydrate, adds 25mL absolute ethyl alcohol, under stirring, drips wherein the aqueous solution 3mL of 4mmoL glycine;
Stir suction filtration after 0.5 hour, with ethanol washing, on silica gel, be dried to constant weight and make rare earth-amino acid two-element match La (Gly)
4cl
33H
2o.
The production method of rich selenium high-quality honeysuckle provided by the invention, at the sprouting period of honeysuckle, will impose on honeysuckle root after farmyard manure and sodium selenite mixing; RE microbial fertilizer, sodium selenite, sodium hydrogensulfite and the surfactant stirring and dissolving that is added to the water, carries out liquid level sprinkling by the mixed liquor of dissolving in prosperous long-term and rice flower bud phase of honeysuckle; Honeysuckle after gathering is dried, obtain honeysuckle finished product; The effect of plant growth regulator is played in the use of rare earth-amino acid two-element match, compared with the micro-fertilizer of traditional rare earth, interior suction conductivity is high, the metabolism of energy coordinate plant growth, promotes the absorption of plant to organic in environment and inorganic nutrients, increases chlorophyll content, improve photosynthesis, promote dry matter transhipment, accumulation and secondary metabolism, reduce heavy metal ion content, improve yield and quality.Not reducing its high-quality effect of increasing production simultaneously, obviously reduce using dosage, improve thus the safety of agricultural production.The use of sodium hydrogensulfite, provides sour environment, promotes the absorption of crop to selenium element, and can suppress photorespiration, promotes dry-matter accumulation; The production method of general rich selenium high-quality honeysuckle is completely by the absorption in honeysuckle process of growth, adopt the method for biological transformation that inorganic selenium is converted into Organic Selenium, the honeysuckle that has solved well traditional cultivation method production only contains basic effective component, after edible, do not reach the problem of mending selenium, safe and reliable, can carry out the production in enormous quantities of honeysuckle, there is stronger propagation and employment and be worth.
Brief description of the drawings
Fig. 1 is the realization flow figure of the production method of the rich selenium high-quality honeysuckle that provides of the embodiment of the present invention.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is described in further detail.Should be appreciated that specific embodiment described herein is only in order to explain the present invention, and be not used in restriction invention.
Fig. 1 shows the realization flow of the production method of the rich selenium high-quality honeysuckle that the embodiment of the present invention provides.
This production method comprises the following steps:
In step S101, at the sprouting period of honeysuckle, will after farmyard manure and sodium selenite mixing, impose on honeysuckle root;
In step S102, RE microbial fertilizer, sodium selenite, sodium hydrogensulfite and the surfactant stirring and dissolving that is added to the water, carries out liquid level sprinkling by the mixed liquor of dissolving for a long time honeysuckle is prosperous;
In step S103, in the rice flower bud phase of honeysuckle, be sprayed on honeysuckle with the mixed liquor of preparing in step S102;
In step S104, the honeysuckle after gathering is dried, obtain honeysuckle finished product.
In embodiments of the present invention, the weight ratio of farmyard manure and sodium selenite is, farmyard manure: sodium selenite=100: 0.001-0.2; Fertilising consumption is the every pier of 2-3kg/.
In embodiments of the present invention, with RE microbial fertilizer, sodium selenite, sodium hydrogensulfite and the surfactant stirring and dissolving that is added to the water, the implementation method that obtains mixed liquor is:
Be uniformly mixed with 10 parts of weight ratio RE microbial fertilizer, sodium selenite 0.01-0.04 part, sodium hydrogensulfite 0.05-0.2 part;
It is that 20-50 DEG C, weight ratio are in the water of 100 parts that fertilizer after being uniformly mixed is joined to temperature, and adds surfactant stirring and dissolving, obtains mixed liquor.
In embodiments of the present invention, RE microbial fertilizer main component is nitrogen, phosphorus, potassium and rare earth-amino acid two-element match, the N in RE microbial fertilizer: P
2o
5: K
2o: the weight ratio of rare earth-amino acid two-element match is 20: 10: 10: 0.001-0.05.
In embodiments of the present invention, the general formula of the rare earth-amino acid two-element match in RE microbial fertilizer is REx (AA) y, wherein x=1-2, y=1-4, rare earth RE in formula is any one trivalent ion in La, Ce, Pm, Nd, (AA) in formula is amino acid, is selected from lysine, threonine, glycine, leucine, isoleucine, valine, methionine, tyrosine, tryptophan, phenyl alanine.
In embodiments of the present invention, at the sprouting period of honeysuckle, by the implementation method that imposes on honeysuckle root after farmyard manure and sodium selenite mixing be: the mixture of farmyard manure and sodium selenite is executed and is embedded in topsoil 20cm, spread thin soil above and cover.
In embodiments of the present invention, while spraying in a prosperous long-term and rice flower bud phase for honeysuckle the mixed liquor being formed by RE microbial fertilizer, sodium selenite, sodium hydrogensulfite and surfactant, after should being chosen in cloudy day or fine day at 4 o'clock in afternoon, spray in latter 24 hours, in case of rainy, fill spray 1 time.
In embodiments of the present invention, when the rare earth-amino acid two-element match in RE microbial fertilizer is La (Gly)
4cl
33H
2when O, rare earth-amino acid two-element match La (Gly)
4cl
33H
2the synthetic method of O is:
Take a certain amount of La
2o
3solid, is dissolved in HCl, allow it fully react and in water-bath slow evaporation to dry;
With acetone washing, be placed on and in NaOH drier, be dried to constant weight and make LaCl
3hydrate;
Accurately take 1mmoL LaCl
3hydrate, adds 25mL absolute ethyl alcohol, under stirring, drips wherein the aqueous solution 3mL of 4mmoL glycine;
Stir suction filtration after 0.5 hour, with ethanol washing, on silica gel, be dried to constant weight and make rare earth-amino acid two-element match La (Gly)
4cl
33H
2o.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
As shown in Figure 1, this production is biological selenium-enriched, and good quality and high output honeysuckle method is carried out in the steps below:
A, after sprouting period when fertilising of honeysuckle adopts farmyard manure and sodium selenite to mix, impose on root, the weight ratio of sodium selenite and farmyard manure is, farmyard manure: sodium selenite=100: 0.001-0.2; Fertilising consumption is the every pier of 2-3kg/;
B, leaf stretching period spray: honeysuckle-leaf growth is prosperous when long, taking RE microbial fertilizer as carrier, add sodium selenite, sodium hydrogensulfite, with 10 parts of weight ratio RE microbial fertilizer, sodium selenite 0.01-0.04 part, sodium hydrogensulfite 0.05-0.2 part is mixed, mixed fertilizer joins temperature 20-50 DEG C, weight ratio is in the water of 100 parts, and adds surfactant stirring and dissolving, and the mixed liquor of dissolving carries out liquid level sprinkling;
C, rice flower bud phase spray: in the rice flower bud phase of honeysuckle, with the mixed liquor of preparing in step b, be sprayed on honeysuckle;
After above-mentioned steps is processed, honeysuckle is dried and gets product after gathering.
In above-mentioned steps a, the mixture of farmyard manure and sodium selenite is executed in topsoil 20cm, spreads thin soil above and covers;
The micro-fertilizer of above-mentioned steps b middle rare earth is mainly the mixture of nitrogen, phosphorus, potassium and rare earth-amino acid two-element match, the N in this RE microbial fertilizer: P
2o
5: K
2o: the weight ratio of rare earth-amino acid two-element match is 20: 10: 10: 0.01-0.5;
In above-mentioned steps b, the general formula of the rare earth-amino acid two-element match in RE microbial fertilizer is REx (AA) y, wherein x=1-2, y=1-4, the rare earth RE in formula is La, Ce, Pm, any one trivalent ion in Nd, (AA) in formula is amino acid, is selected from lysine, threonine, glycine, leucine, isoleucine, valine, methionine, tyrosine, tryptophan, phenyl alanine.
Embodiment 1:
The present invention is a kind of method of producing rich selenium, good quality and high output honeysuckle, the first step: first in the time of the sprouting period fertilising of honeysuckle, after adopting farmyard manure and sodium selenite to mix, execute at honeysuckle root, farmyard manure mixes with sodium selenite by dry with poultry, livestock or people's ight soil, and the weight ratio of farmyard manure and sodium selenite is: farmyard manure: sodium selenite=100: 0.005; Fertilising consumption is the every pier of 3kg/; Farmyard manure is natural material, can provide required nutriment for the growth of gold and silver flower seedling, but mix with sodium selenite after must being dried, otherwise the soluble loss of sodium selenite;
The mixture of farmyard manure and sodium selenite is executed and is embedded in topsoil 20cm, spreads thin soil above and covers.The soluble moisture in soil of sodium selenite, and be easy to flow away downwards, crops seedling stage, spreading thin soil covering is above the effect of playing isolated air, avoids oxidized.
Second step, prosperous long-term honeysuckle, should strengthen benefit selenium, and regulate with rare earth-amino acid plant growth regulator.Taking RE microbial fertilizer as carrier, add sodium selenite, sodium hydrogensulfite, with 10 parts of weight ratio RE microbial fertilizer, sodium selenite 0.001-0.04 part, sodium hydrogensulfite 0.05-0.2 part is mixed, and mixed fertilizer joins temperature 20-50 DEG C, and weight ratio is in the water of 100 parts, and adding organic surface active agent stirring and dissolving, the mixed liquor of dissolving carries out liquid level sprinkling.RE microbial fertilizer is laboratory preparation, wherein, and N: P
2o
5: K
2the weight ratio of O is 20: 10: 10, then adds rare earth-amino acid two-element match La (Gly)
4cl
33H
2o;
Selenium element is more difficult by liquid level absorptance, need to repeatedly spray, but selenium element is easier to be absorbed by plants under sour environment, and sodium hydrogensulfite can provide weak sour environment, can promote the absorption of crop to selenium; Rare earth-amino acid two-element match La (Gly)
4cl
33H
2the synthetic method of O: take a certain amount of La
2o
3solid, is dissolved in HCl, allow it fully react and in water-bath slow evaporation to dry, then with acetone washing, be placed on and in NaOH drier, be dried to constant weight and make LaCl
3hydrate.Accurately take 1mmoL LaCl
3hydrate, adds 25mL absolute ethyl alcohol, under stirring, drips wherein the aqueous solution 3mL of 4mmoL glycine, stirs suction filtration after 0.5 hour, with ethanol washing, is dried to constant weight and makes complex on silica gel.By analysis must this two-element match consist of La (Gly)
4cl
33H
2o.
Sodium selenite, rare earth-amino acid two-element match fertilizer, sodium hydrogensulfite are mixed in proportion, be dissolved in the water of 20-50 DEG C, and add a certain amount of organic surface active agent, honeysuckle is implemented to liquid level and spray.
The 3rd step, the rice flower bud phase sprays: in the rice flower bud phase of honeysuckle, be sprayed on honeysuckle by the mixed liquor of preparing in step b.
In the operation of second step and the 3rd step, when sprinkling, the choosing cloudy day, or after fine day at 4 o'clock in afternoon, spray in latter 24 hours, in case of rainy, fill spray 1 time.
After above-mentioned steps is processed, honeysuckle is dried and gets product after gathering.
Through above-mentioned steps honeysuckle product after treatment, wherein Se content is 786 μ g/kg after measured, and chlorogenic acid and galuteolin content increase respectively 5-15% than untreated sample, and output increases 5-22%.
Embodiment 2:
The production technology of a kind of biological selenium-enriched, good quality and high output honeysuckle of the present invention.
First in the time of the sprouting period fertilising of honeysuckle, adopt farmyard manure and sodium selenite to mix rear root and execute at honeysuckle root, farmyard manure: sodium selenite=100: 0.01; Fertilising consumption is the every pier of 2kg/; The mixture of farmyard manure and sodium selenite is executed and is embedded in topsoil 20cm, spreads thin soil above and covers.
Secondly, gold and silver flower seedling prosperous long-term (i.e. before the rice flower bud phase about 30 days), strengthening benefit selenium, and regulate with rare earth-amino acid plant growth regulator.Taking weight ratio as RE microbial fertilizer 20kg, wherein include La (Gly)
4cl
33H
2o 0.005kg, adds sodium selenite 0.001-0.006kg, and bisulfite 0.005-0.025kg mixes, get mixed fertilizer 0.05kg fertilizer and join temperature 20-50 DEG C, weight ratio is in the water of 100kg, and adds organic surface active agent stirring and dissolving, and the mixed liquor of dissolving carries out liquid level sprinkling.RE microbial fertilizer is laboratory preparation, wherein, and N: P
2o
5: K
2the weight ratio of O is 20: 10: 10, then adds rare earth-amino acid two-element match La (Gly)
4cl
33H
2o.
Finally, the rice flower bud phase sprays: in the rice flower bud phase of honeysuckle, be sprayed on honeysuckle by the mixed liquor of preparing in step b.
In rear two steps operations, when sprinkling, the choosing cloudy day, or after fine day at 4 o'clock in afternoon, spray in latter 24 hours, in case of rainy, fill spray 1 time.
After above-mentioned steps is processed, honeysuckle is dried and gets product after gathering.
Through above-mentioned steps honeysuckle product after treatment, wherein Se content is 762 μ g/kg after measured, and chlorogenic acid and galuteolin content increase respectively 9-19% than untreated sample, and output increases 8-21%.
By above method, with containing 30mgL
-1glycine lanthanum process Pingyi pan honeysuckle flower (mountain honeysuckle flower), can adopt following methods to carry out the mensuration of chlorogenic acid and galuteolin content.
The mensuration of chlorogenic acid:
Chromatographic condition: taking octadecylsilane chemically bonded silica as filler, taking acetonitrile-0.4% phosphoric acid solution (13: 87) as mobile phase, detecting 327nm, is the characteristic peak of 17.9min place for chlorogenic acid in retention time.
The preparation of need testing solution: the about 0.5g of extracting honeysuckle powder (crossing sieve No. four), accurately weighed, put in tool plug conical flask, precision adds 50% methyl alcohol 50mL, weighed weight, ultrasonic processing (power 250w, frequency 35Hz) 30min, let cool, more weighed weight, the weight of supplying less loss with 50% methyl alcohol, shakes up, and filters, precision measures subsequent filtrate 5mL, puts in 25mL brown bottle, adds 50% methyl alcohol to scale, shake up, to obtain final product.The accurate reference substance 10 μ L that draw respectively, injection liquid chromatography, measures, and to obtain final product.Recording without the Chlorogenic Acid of Flos Lonicerae content of glycine lanthanum processing is 2.12%, through containing 30mgL
-1the Chlorogenic Acid of Flos Lonicerae content of glycine lanthanum processing be 2.34%, increased by 10.38% than contrast.
The mensuration of galuteolin:
Chromatographic condition: taking octadecylsilane chemically bonded silica as filler, taking acetonitrile as mobile phase A, taking 0.5% glacial acetic acid solution as Mobile phase B, press 0-30min, mobile phase A: 10%-30%, Mobile phase B: 90%-70% carries out gradient elution, and detection wavelength is 350nm.Be the characteristic peak that 48.1min place is galuteolin in retention time.
The preparation of need testing solution: the about 3g of extracting honeysuckle fine powder (crossing sieve No. four), accurately weighed, put in tool plug conical flask, precision adds 70% ethanol 50mL, weighed weight, ultrasonic processing (power 250w, frequency 35kHz) 1 hour, let cool, more weighed weight, supply the weight of less loss with 70% ethanol, shake up, filter, get subsequent filtrate, to obtain final product.The accurate need testing solution 10 μ L that draw, injection liquid chromatography, measures, and to obtain final product.Recording without galuteolin content in the honeysuckle of glycine lanthanum processing is 0.14%, through containing 30mgL
-1the honeysuckle of glycine lanthanum processing in galuteolin content be 0.16%, increased by 14.28% than contrast.
The production method of the rich selenium high-quality honeysuckle that the embodiment of the present invention provides, at the sprouting period of honeysuckle, will impose on honeysuckle root after farmyard manure and sodium selenite mixing; RE microbial fertilizer, sodium selenite, sodium hydrogensulfite and the surfactant stirring and dissolving that is added to the water, carries out liquid level sprinkling by the mixed liquor of dissolving in prosperous long-term and rice flower bud phase of honeysuckle; Honeysuckle after gathering is dried, obtain honeysuckle finished product; The effect of plant growth regulator is played in the use of rare earth-amino acid two-element match, compared with the micro-fertilizer of traditional rare earth, interior suction conductivity is high, the metabolism of energy coordinate plant growth, promotes the absorption of plant to organic in environment and inorganic nutrients, increases chlorophyll content, improve photosynthesis, promote dry matter transhipment, accumulation and secondary metabolism, reduce heavy metal ion content, improve yield and quality.Not reducing its high-quality effect of increasing production simultaneously, obviously reduce using dosage, improve thus the safety of agricultural production.The use of sodium hydrogensulfite, provides sour environment, promotes the absorption of crop to selenium element, and can suppress photorespiration, promotes dry-matter accumulation; The production method of general rich selenium high-quality honeysuckle is completely by the absorption in honeysuckle process of growth, adopt the method for biological transformation that inorganic selenium is converted into Organic Selenium, the honeysuckle that has solved well traditional cultivation method production only contains basic effective component, after edible, do not reach the problem of mending selenium, safe and reliable, can carry out the production in enormous quantities of honeysuckle, there is stronger propagation and employment and be worth.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (2)
1. a production method for rich selenium high-quality honeysuckle, is characterized in that, this production method comprises the following steps:
Step 1, at the sprouting period of honeysuckle, will impose on honeysuckle root after farmyard manure and sodium selenite mixing;
Step 2, RE microbial fertilizer, sodium selenite, sodium hydrogensulfite and the surfactant stirring and dissolving that is added to the water, carries out foliage spray by the mixed liquor of dissolving for a long time honeysuckle is prosperous;
Step 3, in the rice flower bud phase of honeysuckle, is sprayed on honeysuckle with the mixed liquor of preparing in step 2;
Step 4, dries the honeysuckle after gathering, and obtains honeysuckle finished product;
The weight ratio of farmyard manure and sodium selenite is, farmyard manure: sodium selenite=100: 0.001-0.2; Fertilising consumption is that 2-3kg/ is per ton;
With RE microbial fertilizer, sodium selenite, sodium hydrogensulfite and the surfactant stirring and dissolving that is added to the water, the implementation method that obtains mixed liquor is:
Be uniformly mixed with 10 parts of weight ratio RE microbial fertilizer, sodium selenite 0.01-0.04 part, sodium hydrogensulfite 0.05-2 part;
It is that 20-50 DEG C, weight ratio are in the water of 100 parts that fertilizer after being uniformly mixed is joined to temperature, and adds surfactant stirring and dissolving, obtains mixed liquor;
RE microbial fertilizer main component is nitrogen, phosphorus, potassium and rare earth-amino acid two-element match, the N in RE microbial fertilizer: P
2o
5: K
2o: the weight ratio of rare earth-amino acid two-element match is 20: 10: 10: 0.001-0.05;
The general formula of the rare earth-amino acid two-element match in RE microbial fertilizer is REx-(AA) y, wherein x=1-2, y=1-4, rare earth RE in formula is any one trivalent ion in La, Ce, Pm, Nd, (AA) in formula is amino acid, is selected from lysine, threonine, glycine, leucine, isoleucine, valine, methionine, tyrosine, tryptophan or phenyl alanine;
At the sprouting period of honeysuckle, by the implementation method that imposes on honeysuckle root after farmyard manure and sodium selenite mixing be: the mixture of farmyard manure and sodium selenite is executed and is embedded in topsoil 20cm, spread thin soil above and cover;
In rice flower bud phase of honeysuckle while spraying the mixed liquor being formed by RE microbial fertilizer, sodium selenite, sodium hydrogensulfite and surfactant, after should being chosen in cloudy day or fine day at 4 o'clock in afternoon, spray in latter 24 hours, in case of rainy, fill spray 1 time.
2. production method as claimed in claim 1, is characterized in that, when the rare earth-amino acid two-element match in RE microbial fertilizer is La (Gly)
4cl
33H
2when O, rare earth-amino acid binary coordinates La (Gly)
4cl
33H
2the synthetic method of O is:
Take a certain amount of La
2o
3solid, is dissolved in HCl, allow it fully react and in water-bath slow evaporation to dry;
With acetone washing, be placed on and in NaOH drier, be dried to constant weight and make LaCl
3hydrate;
Accurately take 1mmoL LaCl
3hydrate, adds 25mL absolute ethyl alcohol, under stirring, drips wherein the aqueous solution 3mL of 4mmoL glycine;
Stir suction filtration after 0.5 hour, with ethanol washing, on silica gel, be dried to constant weight and make rare earth-amino acid two-element match La (Gly)
4cl
33H
2o.
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| CN114830977A (en) * | 2022-04-24 | 2022-08-02 | 麻城市德应米业股份有限公司 | Production method of green selenium-rich nutritional rice |
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| CN102424634A (en) * | 2011-09-18 | 2012-04-25 | 山东鲁峰金银花有限公司 | Selenium-enriched special fertilizer for honeysuckle flower and production method thereof |
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