CN104016752B - A kind of nutritional solution for improving pfaffia Root yield and application thereof - Google Patents
A kind of nutritional solution for improving pfaffia Root yield and application thereof Download PDFInfo
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Abstract
The present invention relates to field of plant cultivation, particularly to a kind of nutritional solution for improving pfaffia Root yield and application thereof. A kind of nutritional solution for improving pfaffia Root yield, described nutritional solution mainly consists of the following composition: sodium selenite 300mg/L～500mg/L, chitosan 30mg/L～50mg/L, sodium sulfite 250mg/L～400mg/L, solvent is water. This nutrient solution mode is simple, no side effects, and can significantly improve the yield of pfaffia root. While the nutritional solution using the present invention sprays pfaffia volume increase, its main component saponin content is stable.
Description
Technical field
The present invention relates to field of plant cultivation, particularly to a kind of nutritional solution for improving pfaffia Root yield and application thereof.
Background technology
Pfaffia (Pfaffiapaniculata) is also known as enamel phenanthrene sub-(Pfaffia), for Amaranthaceae (Amaranthaceae) Achyranthes herbaceos perennial, it is used as medicine with root, there is the effects such as heat-clearing and toxic substances removing, powerful tonifying YANG, anti-inflammatory and antalgic. It is mainly used in section's diseases such as treatment cardiovascular, nervus centralis, reproduction, digestive system. Enamel phenanthrene Asia originates in the Tropical rain forest area of the states such as South America Brazil. China has introduced a fine variety at present and has succeeded. Enamel phenanthrene subgenus sun plant, is generally grown in the place that sunlight is comparatively sufficient. Plant height 150cm-200cm, the usual 3-5 bar of root, cylindrical, in yellow. Cane is made up of some joints, and internode is longer, hollow, and the stem of joint is that knee is expanded, and side shoot is to life. The growth of enamel phenanthrene Asia is unbalanced, and nutrient substance distribution is inharmonious, and economic coefficient is low. At vegetative growth phase, the Asia aerial growth of enamel phenanthrene is rapid, and plant is tall and big; Under ground portion hypoevolutism, the growth of root is significantly restricted, root/shoot ratio numerous imbalances (up to 1:12), the particularly prosperous length of aerial parts stem, consume substantial amounts of nutrient substance, blade usually comes off in a large number, and plant is fragile, meets wind and easily lodges, seriously constrain the development in enamel phenanthrene Asia photosynthetic " source ", greatly reduce the quantity in " source ", add the nutrient competition in strong " storehouse " of stem, the impact of enamel phenanthrene Asia root yield is very big.
The present inventor has carried out substantial amounts of research and experiment for the problems referred to above in the process carrying out pfaffia cultivation, it is found surprisingly that the nutritional solution that adopts a kind of human configuration to spray plant in the moment of pfaffia seedling early growth and not only avoid the generation of the problems referred to above, but also improves the yield of pfaffia medical material.
Summary of the invention
The present invention provides a kind of nutritional solution for improving pfaffia Root yield, and this nutrient solution mode is simple, no side effects, and can significantly improve the yield of pfaffia root.
The preparation method that the present invention also provides for a kind of described nutritional solution.
The present invention also provides for a kind of method improving pfaffia Root yield.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of nutritional solution for improving pfaffia Root yield, described nutritional solution mainly consists of the following composition: sodium selenite 300mg/L～500mg/L, chitosan 30mg/L～50mg/L, sodium sulfite 250mg/L～400mg/L, solvent is water.This nutrient solution mode is simple, no side effects, and can significantly improve the yield of pfaffia root.
Described nutritional solution consists of the following composition: sodium selenite 350mg/L～450mg/L, chitosan 35mg/L～45mg/L, sodium sulfite 300mg/L～380mg/L, solvent is water.
Described nutritional solution consists of the following composition: sodium selenite 400mg/L, chitosan 45mg/L, sodium sulfite 350mg/L, and solvent is water.
A kind of preparation method of described nutritional solution, the method, particularly as follows: weigh sodium sulfite, sodium selenite and chitosan respectively, adds three kinds of compositions successively in water, stirs while adding dissolving, the addition sequence of three kinds of compositions is sodium sulfite, sodium selenite, chitosan, last constant volume.
A kind of described nutritional solution is for improving the application of pfaffia Root yield.
A kind of method improving pfaffia Root yield, uniformly sprays pfaffia plant between April to July with nutritional solution of the present invention, spray frequency be 4～20 days once.
Further, spray with the described nutritional solution front to the blade face of pfaffia plant and/or the back side, front and/or the back side and root.
The invention has the beneficial effects as follows: the nutritional solution of the present invention can remarkably promote the growth of pfaffia root and improve the yield of medical material. While the nutritional solution using the present invention sprays pfaffia volume increase, its main component saponin content is stable.
Detailed description of the invention
Below by specific embodiment, technical scheme is described in further detail. Should be appreciated that the enforcement of the present invention is not limited to the following examples, any pro forma accommodation and/or change that the present invention is made fall within scope.
In the present invention, if not refering in particular to, all of part, percentage ratio are unit of weight, and the equipment adopted and raw material etc. all can be buied from market or commonly used in the art. Method in following embodiment, if no special instructions, is the conventional method of this area.
" mg/L " writing a Chinese character in simplified form for unit " mg/litre " in the present invention, is construed to: preparing complete volume is the weight containing the materials such as sodium selenite in one liter of solution; " liter " with volume be 1 liter graduated cylinder for normal capacity; " mg " writes a Chinese character in simplified form for unit " milligram ", with the weight of the materials such as the sodium selenite that applying electronic balance weighs.
Sodium sulfite: molecular formula NaHSO3, molecular weight 104.06, purity >=99.5%;
Sodium selenite: molecular formula Na2SeO3, molecular weight 172.94, purity >=98%
Chitosan: also known as chitosan, molecular formula: C56H103N9O39, molecular weight: 1526.4539, purity >=99%.
Experimental example
Experimental example 1 contrasts paclobutrazol, sodium selenite, chitosan, sodium sulfite are used alone the effect that pfaffia promotes root growth
Paclobutrazol working concentration in the operation of this experimental example and mode according to being " paclobutrazol impact on enamel phenanthrene Asia growth promoter and root yield ", Ling Zhengzhu etc., time treasure's traditional Chinese medical science traditional Chinese medicines the 21st volume the 4th phase in 2010.
1, cuttage processes
The healthy and strong plant of selected annual pfaffia, cut middle part stem section and did cutting, in greenhouse sand bed cuttage, treat that cutting seedling grows 6 leaves, seedling is transplanted to sample plot mid-March. Transplant first 1～2 day to combine and plough deeply, use base fertilizer. Test adopts randomized block design, seeding row spacing 40cm × 40cm, every district 4m2, every district field planting 36 strain.Mid-May, pfaffia initially enter prosperous for a long time, then pfaffia carried out front, blade face uniformly spray different types of nutritional solution according to requirement of experiment, till dripping in blade face, within every four days, spray once. Then, the big and heavy of root is measured in October.
2, nutritional solution kind and concentration thereof are arranged
Paclobutrazol: for the experiment product of commercially available purity >=98%; Sodium selenite: for the experiment product of commercially available purity >=98%; Chitosan: for the experiment product of commercially available purity >=99%; Sodium sulfite, for the experiment product of commercially available purity >=99.5%.
This experiment is selected four kinds of additives to be individually configured to nutritional solution and is sprayed front and the back side of Brazilian's Rhizoma panacis majoris, within every four days, sprays once, and the concentration that each additive is arranged is as shown in table 1. Meanwhile, with the tap water without any additive for comparison (CK). Experiment being taken off with the root of plant and washed at the beginning of 10 months, then measure fresh weight respectively, then calculate the average weight of every strain pfaffia root.
The preparation of each nutritional solution:
Compound concentration is the paclobutrazol solution of 300mg/L: with electronic balance accurate measuring 300mg paclobutrazol, 1L beaker adds about 500ml tap water or pure water, then the 300mg paclobutrazol measured is added in beaker, stirring while adding until all dissolving, finally this solution all being poured in the graduated cylinder that volume is 1L standby after constant volume again.
All the other each solution of additive manner of formulation are identical with the paclobutrazol solution allocation mode of above-mentioned 300mg/L, namely join and namely use.
Be can be seen that by result in table 1, behind the blade face individually spraying pfaffia with each additive, root heavily be there is no the effect of being obviously promoted, otherwise, when paclobutrazol concentration reach 500mg/L, concentration of sodium selenite reaches 500mg/L, chitosan concentration reaches 50mg/L time, the fresh weight of pfaffia root is suppressed on the contrary. Not finding that pfaffia root growth is played obvious facilitation when 300mg/L by paclobutrazol, " paclobutrazol impact on enamel phenanthrene Asia growth promoter and root yield " research that this delivers from Ling Zhengzhu etc. is different. In a word, find in the research of the applicant, individually add the additive in table 1 substantially without promoting pfaffia root growth, namely do not increase the yield of pfaffia medical material.
The independent additive impact on pfaffia root growth of table 1
Experimental example 2 contrasts paclobutrazol, sodium selenite, chitosan, the sodium sulfite combination effect to pfaffia promotion root growth
This experimentation is for the impact on pfaffia root growth of the compound mode of additive, and wherein, kind and the concentration thereof of additive are as shown in table 2. Cutting seedling, spray mode and number of times, experiment strain number, gravimetry and calculate all identical with experimental example 1.
Solution preparation mode containing additive:
The solution preparation mode of 1,2,4 of being numbered is without particularity, have only to weigh aequum additive respectively according to concentration requirement electronic balance, then each additive is taked the mode stirred while adding pour in the beaker filling pure water or tap water, until all dissolving, finally this solution is all poured in the graduated cylinder that volume is 1L standby after constant volume again.
It is numbered the solution preparation mode of 3,5,6, for numbering 3 solution: first weigh the paclobutrazol of 300mg/L with electronic balance, then the mode that paclobutrazol stirs while adding poured in the beaker filling pure water or tap water, until all dissolving;Weigh the chitosan of 30mg again, stirring while adding in this solution of addition, until all dissolving, finally this solution all being poured in the graduated cylinder that volume is 1L standby after constant volume again. All the other solution containing shell sugar composition are both needed to prepare solution according to the mode of numbering 3.
By result in table 2 it can be seen that after using two kinds of additive combination solutions to spray the blade face of pfaffia, root heavily be there is no the effect of being obviously promoted.
The 2 two kinds of additive combinations of the table impact on pfaffia root growth
Experimental example 3 contrasts paclobutrazol, sodium selenite, chitosan, the three kinds of composition combinations of the sodium sulfite effect to pfaffia promotion root growth
Experiment purpose and method: this experimentation is for the impact on pfaffia root growth of the compound mode of additive, and wherein, kind and the concentration thereof of additive are as shown in table 3. Cutting seedling, spray mode and number of times, experiment strain number, gravimetry and calculate all identical with experimental example 1.
Solution preparation mode containing additive: be numbered in 2,3,4 solution and all contain chitosan, when preparing solution, as shown in experimental example 2, chitosan need to add after all the other additives dissolve, finally standby with graduated cylinder constant volume.
Data as shown in Table 3 are it can be seen that these four additive is configured to after solution sprays Brazilian's Rhizoma panacis majoris after every three kinds of combinations, and only the solution of numbering 4 has and is obviously promoted the effect that pfaffia root fresh weight increases, and it is inconspicuous that all the other combine dry root weight increase.
The 3 three kinds of additive combinations of the table impact on pfaffia root growth
The additive of experimental example 4 variable concentrations combines the impact on pfaffia root growth
Experiment purpose and method: this experimentation is for the impact on pfaffia root growth of the concentration of additive, and wherein, kind and the concentration thereof of additive are as shown in table 4. Cutting seedling, spray mode and number of times, experiment strain number, gravimetry and calculate all identical with experimental example 1.
Solution preparation mode containing additive: except CK, all contains chitosan in solution, when preparing solution, as shown in experimental example 2, chitosan need to add after all the other additives dissolve, finally standby with graduated cylinder constant volume.
As can be seen from Table 4: three kinds of additive compositions are respectively within the scope of finite concentration and with the use of just playing the effect promoting pfaffia root to increase weight and to improve yield, such as, when concentration of sodium selenite reaches 550mg/L, inhibit the weight of pfaffia root on the contrary; When the concentration of chitosan reaches 60mg/L, this combination nutrient liquid is not notable to pfaffia dry root weight increase.
The additive of table 4 variable concentrations combines the impact on pfaffia root growth
Embodiment
Embodiment 1
The healthy and strong plant of selected annual pfaffia, cut middle part stem section and did cutting, in greenhouse sand bed cuttage, treat that cutting seedling grows 6 leaves, seedling is transplanted to sample plot mid-March. Transplant first 1～2 day to combine and plough deeply, use base fertilizer. Test adopts randomized block design, seeding row spacing 40cm × 40cm, every district 12m2, every district field planting 100 strain. Mid-June, pfaffia carrying out the uniform spray nutritious liquor of blade face obverse and reverse, wherein, the composition of nutritional solution is: sodium selenite 300mg/L, sodium sulfite 400mg/L, chitosan 45mg/L, surplus are water. The manner of formulation of this nutritional solution such as identical described in experimental example. Till dripping in blade face, within every 8 days, spray once. Then, the big and heavy of root is measured and to calculate the counterpoise of every strain pfaffia root be 63.36mg in October.
Embodiment 2
The healthy and strong plant of selected annual pfaffia, cut middle part stem section and did cutting, in greenhouse sand bed cuttage, treat that cutting seedling grows 6 leaves, seedling is transplanted to sample plot mid-March. Transplant first 1～2 day to combine and plough deeply, use base fertilizer. Test adopts randomized block design, seeding row spacing 40cm × 40cm, every district 12m2, every district field planting 100 strain. Mid-April, pfaffia carrying out the uniform spray nutritious liquor of blade face positive and negative and root cultivation matrix surface, the composition of nutritional solution is: sodium selenite 500mg/L, sodium sulfite 250mg/L, chitosan 30mg/L, surplus are water. The manner of formulation of this nutritional solution such as identical described in experimental example. Till dripping in blade face, within every 12 days, spray once. Then, the big and heavy of root is measured and to calculate the counterpoise of every strain pfaffia root be 62.35mg in October.
Embodiment 3
The healthy and strong plant of selected annual pfaffia, cut middle part stem section and did cutting, in greenhouse sand bed cuttage, treat that cutting seedling grows 6 leaves, seedling is transplanted to sample plot mid-March. Transplant first 1～2 day to combine and plough deeply, use base fertilizer. Test adopts randomized block design, seeding row spacing 40cm × 40cm, every district 12m2, every district field planting 100 strain. Mid-April, pfaffia carrying out the uniform spray nutritious liquor of blade face reverse side and root cultivation matrix surface, the composition of nutritional solution is: sodium selenite 350mg/L, sodium sulfite 380mg/L, chitosan 45mg/L, surplus are water. The manner of formulation of this nutritional solution such as identical described in experimental example. Till dripping in blade face, within every 20 days, spray once. Then, measure the big and heavy of root in October and calculate the counterpoise 62.13mg of every strain pfaffia root.
Embodiment 4
The healthy and strong plant of selected annual pfaffia, cut middle part stem section and did cutting, in greenhouse sand bed cuttage, treat that cutting seedling grows 6 leaves, seedling is transplanted to sample plot mid-March. Transplant first 1～2 day to combine and plough deeply, use base fertilizer. Test adopts randomized block design, seeding row spacing 40cm × 40cm, every district 12m2, every district field planting 100 strain. Mid-April, pfaffia carrying out blade face just and the uniform spray nutritious liquor in root cultivation matrix surface, the composition of nutritional solution is: sodium selenite 450mg/L, sodium sulfite 300mg/L, chitosan 35mg/L, surplus are water. The manner of formulation of this nutritional solution such as identical described in experimental example. Till dripping in blade face, within every 20 days, spray once. Then, the big and heavy of root is measured and to calculate the counterpoise of every strain pfaffia root be 65.53mg in October.
Embodiment 5
The healthy and strong plant of selected annual pfaffia, cut middle part stem section and did cutting, in greenhouse sand bed cuttage, treat that cutting seedling grows 6 leaves, seedling is transplanted to sample plot mid-March. Transplant first 1～2 day to combine and plough deeply, use base fertilizer. Test adopts randomized block design, seeding row spacing 40cm × 40cm, every district 12m2, every district field planting 100 strain. Mid-April, pfaffia only carrying out the uniform spray nutritious liquor in front, blade face, the composition of nutritional solution is: sodium selenite 400mg/L, sodium sulfite 350mg/L, chitosan 45mg/L, surplus are water. The manner of formulation of this nutritional solution such as identical described in experimental example. Till dripping in blade face, within every 4 days, spray once. Then, measure the big and heavy of root in October and calculate the counterpoise 67.55mg of every strain pfaffia root.
Embodiment 6
Randomly select without the pfaffia root in spray nutritious liquor process and embodiment 5 standby after drying.
The preparation of 1 solution
Standard solution prepares accurately weighed oleanolic acid reference substance, adds methanol and is configured to the solution of 0.29mg/ml, as reference substance solution.
The preparation of need testing solution:
Precision weighs the powder 2g of the dry constant weight of pfaffia root processed and in embodiment 5 without spray nutritious liquor, in 250ml flask, add 80% ethanol 50ml, microwave extraction, filter, take subsequent filtrate 25ml, the 10ml that adds water after water bath method dissolves, with the n-butanol extraction 3 times of water saturation, and 15ml/ time, merge butanol extraction liquid, again with the water washing of the saturated mistake of 15ml n-butyl alcohol, collect n-butyl alcohol liquid, dissolve with methanol after flinging to n-butyl alcohol, put in 50ml volumetric flask, and by methanol constant volume to scale.
2 selections measuring wavelength
Taking the oleanolic acid comparison liquid of 0.5ml, be evaporated in water-bath dry, add the 5% vanillin-glacial acetic acid 0.2ml of new preparation, perchloric acid 0.8ml, close plug, after 70 DEG C of water-baths develop the color 15min, circulating water is cooled to room temperature. Adding ethyl acetate 5ml, shake up, scan at ultraviolet spectrophotometer immediately under 400～800nm wavelength, blank solution makes reference simultaneously, has absorption maximum at 542nm place, thereby determines that detection wavelength is 542nm.
3 linear relationships are investigated
The above-mentioned reference substance solution 0.00,0.10,0.20,0.30 of accurate absorption, 0.40,0.50ml, water-bath is evaporated to dry, adds 5% vanillin-glacial acetic acid (facing with newly joining) 0.2ml, perchloric acid 0.8ml, after 70 DEG C of water-baths develop the color 15min, puts and is cooled to room temperature in circulating water. Adding ethyl acetate 5ml, shake up, blank assay is done in retinue, measures absorbance at 542nm place. Calculating regression equation is: A=6.1775C-0.0089, r=0.9990, in good linear relationship within the scope of 0.0058～0.029mg/ml.
4 stability tests
Accurate oleanolic acid reference substance solution (0.29mg/ml) 0.2ml that draws, in tool plug test tube, develops the color by standard curve method, and blank made by retinue reagent, measures in the timing of 542nm wavelength place, measures 1 absorbance every 5min, measures 30min altogether. After reference substance solution colour developing, absorbance is stable in 5～30min, RSD=0.068%, therefore colorimetric determination completes impact not quite in 30min.
5 Precision Experiment precisions draw oleanolic acid comparison liquid 0.20ml, by operation 5 times under " 6.3 " item, measure absorbance. Calculating RSD is 0.051%.
6 sample sizes measure
Precision measures sample solution 0.4ml, by operation under " 4 stability test " item, carries out assay.
7 results and analysis
The pfaffia root saponin content spraying process without nutritional solution is 6.78%, and spraying the pfaffia root saponin content of process through nutritional solution in embodiment 5 is 6.81%.
From the above results, while the nutritional solution using the present invention sprays pfaffia volume increase, its main component saponin content is stable.
Embodiment described above is the one preferably scheme of the present invention, not the present invention is done any pro forma restriction, also has other variant and remodeling under the premise without departing from the technical scheme described in claim.
Claims (7)
1. the nutritional solution being used for improving pfaffia Root yield, it is characterized in that described nutritional solution comprises the following ingredients: sodium selenite 300mg/L～500mg/L, chitosan 30mg/L～50mg/L, sodium sulfite 250mg/L～400mg/L, solvent is water.
2. nutritional solution according to claim 1, it is characterised in that described nutritional solution consists of the following composition: sodium selenite 350mg/L～450mg/L, chitosan 35mg/L～45mg/L, sodium sulfite 300mg/L～380mg/L, solvent is water.
3. nutritional solution according to claim 1, it is characterised in that described nutritional solution consists of the following composition: sodium selenite 400mg/L, chitosan 45mg/L, sodium sulfite 350mg/L, solvent is water.
4. the preparation method of the nutritional solution described in a claim 1, it is characterized in that the method particularly as follows: weigh sodium sulfite, sodium selenite and chitosan respectively, three kinds of compositions are added successively in water, stir while adding dissolving, the addition sequence of three kinds of compositions is sodium sulfite, sodium selenite, chitosan, last constant volume.
5. the nutritional solution described in a claim 1 is for improving the application of pfaffia Root yield.
6. the method improving pfaffia Root yield, it is characterised in that: pfaffia plant is uniformly sprayed between April to July with the nutritional solution described in claim 1, spray frequency be 4～20 days once.
7. the method for raising pfaffia Root yield according to claim 6, it is characterised in that: spray with the described nutritional solution front to the blade face of pfaffia plant and/or the back side, front and/or the back side and root.
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Citations (3)
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| CN101157581A (en) * | 2007-09-21 | 2008-04-09 | 长沙三元催化剂有限公司 | Selenium-enriched organic nourishment modifier and method for making same |
| CN101250075A (en) * | 2008-03-18 | 2008-08-27 | 吉林农业大学 | Ginseng foliar fertilizer |
| CN102701823A (en) * | 2012-06-08 | 2012-10-03 | 临沂大学 | Production method of selenium-rich high-quality honeysuckle |
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| KR100729713B1 (en) * | 2006-05-24 | 2007-06-18 | 재단법인 전라북도생물산업진흥원 | Fetilizer containing stevia-extracts and mineral matter and |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101157581A (en) * | 2007-09-21 | 2008-04-09 | 长沙三元催化剂有限公司 | Selenium-enriched organic nourishment modifier and method for making same |
| CN101250075A (en) * | 2008-03-18 | 2008-08-27 | 吉林农业大学 | Ginseng foliar fertilizer |
| CN102701823A (en) * | 2012-06-08 | 2012-10-03 | 临沂大学 | Production method of selenium-rich high-quality honeysuckle |
Non-Patent Citations (1)
| Title |
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| 多效唑对珐菲亚生长发育和药材产量的影响;凌征柱 等;《时珍国医国药》;20100420;第21卷(第4期);第992页第2.1节 * |
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