CN102696390A - Method for promoting budding of seedling budlings of Brazil rubber trees - Google Patents
Method for promoting budding of seedling budlings of Brazil rubber trees Download PDFInfo
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- CN102696390A CN102696390A CN2012102253049A CN201210225304A CN102696390A CN 102696390 A CN102696390 A CN 102696390A CN 2012102253049 A CN2012102253049 A CN 2012102253049A CN 201210225304 A CN201210225304 A CN 201210225304A CN 102696390 A CN102696390 A CN 102696390A
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Abstract
The invention discloses a method for promoting budding of seedling budlings of Brazil rubber trees. The method comprises the following steps of: topping pretreatment of seedling budlings, toping and bud removal for tree stock, budding promoter application to bud slices and culturing of strong seedlings. The method is simple in process flow and low in production cost, has no adverse effect on growth of seedlings, well promotes the budding effect, is stable in field performance, better solves the problems of slow budding and uneven budding of seedling budlings of Brazil rubber trees, is applicable to seedling budlings of Brazil rubber trees, which are grafted with buds and overwinter with apical buds left, lays a foundation for popularization of industrial seedling culturing of rubber trees.
Description
Technical field
The present invention relates to a kind of method that promotes that Para rubber tree budding seedling sprouts, belong to the crop cultivating technology field.
Background technology
Bamboo grows budding technology is the major technique means that employing is bred in the bamboo grows batch production, and this technology nursery stage is short, and land utilization rate is high, has effectively reduced material and labor cost, is the existing technology of promoting mainly.In the production practices of budding technology; Owing to the budling quality, cut the influence of factors such as bar mode, cultivation management measure and external condition; Its sprout rate is unstable, sprouts that it is longer to go through the phase, and the ratio of not sprouting is on average 8.5%; During miscarriage not sprout rate can reach more than 30%, influenced the regularity and the outplanting rate of seedling growth.Promote the method that the bamboo grows budling is sprouted; Huang Shoufeng, quality of stand husband and Xie Guishui etc. study per capita; Analyze influencing the factor that the bamboo grows budding sprouts, drawn preliminary conclusion, and affirmed that the basic element of cell division handles the effect of budling to promoting that the budding seedling sprouts; Also having provided with 6-BA is the prescription of main active ingredient, but fails on producing, to apply.The efficient branching promoter for plant that Luoyang woods section is developed it is reported and on the fruit-tree grafting seedling, uses, and can obtain the effect of sprouting preferably, but uses on bamboo grows budding seedling through test, and effect and blank do not have significant difference; It is coated with the method for bud picking sheet, but can use for reference.
Summary of the invention
The objective of the invention is in order to overcome the deficiency of prior art, a kind of method that promotes that bamboo grows budding seedling sprouts is provided, make that bamboo grows budding seedling sprouts neatly, robust growth, reduce invalid seedling ratio, shorten seedling raise period and improve outplanting rate.
For the attainment of one's purpose, a kind of method that promotes that bamboo grows budding seedling sprouts provided by the invention, this method comprises:
1), the pre-treatment of pinching of budding seedling: pinch the last week at the budding seedling, the thin liquid manure that pouring is macerated system with chicken manure, peanut press pulp and phosphate fertilizer once, and the foliage-spray ammino acid liquid fertilizer is once;
2), stock is pinched and the stock bud removes and to sprout: the budding seedling of normal bud grafting in season, 25～30d after bud grafting, the budding seedling that bud grafting becomes to live keeps first pair of true leaf and plucks the top, and the use scraper excavates the axillalry bud of rough leaf, separates simultaneously and ties up; The budding seedling that stays the top to survive the winter; Temperature gos up and pinches operation when being stabilized in more than 15 ℃; It is aging or use sharp secateurs above first pair of true leaf, to cut off at the 1cm place when just having sprouted to select stock phenology to be in the ceiling leaf; And use scraper to excavate the axillalry bud of rough leaf, and separate simultaneously and tie up, separate and want timely bud picking after tying up;
3), use the promoter of sprouting to handle the bud sheet: respectively with 6-BA and GA
3Be dissolved in the 0.1mol/LHCl solution, being mixed with volumetric concentration is the 6-BA solution of 35～45mg/L and the GA of 10～15 mg/L
3Solution; Respectively TDZ and NAA are dissolved in that to be mixed with volumetric concentration in the 0.1mol/LNaOH solution be the TDZ solution of 75～85mg/L and the NAA solution of 25～35mg/L; Again they are mixed; Use 0.1mol/LHCl solution or 0.1mol/LNaOH solution to regulate PH to 5.8～7.0 and promptly get the promoter of sprouting; The promoter of will sprouting adds the Tween 80 or the agricultural spreader-sticker of volume ratio 0.02%, shakes up, and existing mixing at present used; Wherein, use absorbent cotton to dip in the promoter of sprouting on a small scale, directly be coated with the bud picking sheet, be not gathered into water droplet degree of being with the moistening soup of whole bud sheet; Can the promoter of sprouting be diluted to a half strength in enormous quantities, carry out directed spraying after the use knapsack sprayer installs protective cover additional, squirt stock budding panel zone, avoid being sprayed onto blade and get final product; Handle in the 24h of back and do not water, meet rain in the outdoor cropping nursery stock 4h, need to stop using the soup coating of half strength after budding panel does not have ponding or filling spray once at rain;
4), strong seedling cultivation: pinch one week of back, top application is become thoroughly decomposed fertilizer once, and is affixed by liquid manure, and every first quarter moon is used foliage fertilizer once, keeps compost moistening, to promote to budder sprouting; Bud picking regularly, erase stock cotyledon axillalry bud and other sprout tillers destroy apical dominance, let the nutrient centralizedly supply budder; Prevention and elimination of disease and pests in time, the main damage by disease and insect that takes place are the secondary pearl lecanium of Eotetranychus sexmaculatus, rubber, powdery mildew, anthracnose; Physiological disease mainly is a nutritional deficiency, and wherein iron deficiency and magnesium deficiency are comparatively common, can and handle according to symptom identification; Budling survives the winter, and budling need place in the greenhouse and cultivate autumn, newly takes out budlet ability safe overwintering in the normal time, and like consecutive low temperature weather, the processing of then need heating in the greenhouse guarantees mean temperature more than 15 ℃, and minimum temperature is more than 8 ℃.
Introduce new plant growth regulator TDZ (Thidiazuron) as sprouting the main active ingredient of promoter, be used 6-BA (6-benzyl aminoadenine), GA
3(gibberellin) and NAA (a-methyl) reach and promote the healthy and strong effect of buddering and the method for using the bud sheet to smear; Dosage is few, and effect stability can make the budding seedling stalwartness of sprouting; Sprout promoter under by the concentration requirement operating position, have no adverse reaction, the high concentration use can be induced the first fluffy leaf scale bud petiole growth of buddering; But can not promote to budder produces the bud of growing thickly, and can recover normal when giving birth to the second fluffy leaf buddering to take out, and possesses higher safety.
The bamboo grows budding seedling provided by the present invention promotion method of sprouting; Guaranteeing under the situation of bud sheet and stock quality in early stage, can make budling 85% the phase of going through of sprouting shorten to 45 days, handling back 60 days by 120 days; Budling is sprouted and can reach more than 95%; Can shorten the budding growing-seedling period of growing seedlings, improve the budding seedling regularity that sprouts, improve the outplanting rate.
Technological process of the present invention is simple, and is workable, and effect stability and production cost are low, possess promotion advantage.Bamboo grows budding seedling sprout the popularization of promotion method will effectively improve the budding seedling sprout regularity, reduce invalid seedling ratio, shorten seedling raise period, improve the outplanting rate; Help the further perfect of bamboo grows budding technology, for the bamboo grows factorial seedling growth lays the foundation.
Embodiment
With embodiment the present invention is described further below.
1, stock and budling
Stock is selected the embryo seedling through the vernalization of husky bed, and in after planting about 2 ~ 3 weeks, growth of seedling is healthy and strong, and 15 ~ 30 centimetres of stem height are in elongating stage to the leaf stretching period stage.Budling is green budwood, recommends to use specialty to breed the small-sized green budwood that the garden produces, and also can use conventional propagation garden to segment branch and the tissue culture budling through identifying.
2, bud grafting and budling field planting
Use conventional budding method to carry out, handle with care in the bud grafting process as far as possible, protection stock seed is also avoided mechanical wounding.The straight field planting privately of the seedling that bud grafting is good is in Seedling bag central authorities, and the field planting degree of depth soil face degree of depth is concordant with nursery stock rhizome junction, covers the sunshade net, keeps ground moistening, and is healthy and strong to promote growth of seedling.
3, the budding seedling pre-treatment of pinching.
Pinch the last week at the budding seedling, the thin liquid manure that pouring is macerated system with chicken manure, peanut press pulp and phosphate fertilizer once, and the foliage-spray ammino acid liquid fertilizer is once.
4, stock is pinched and the stock bud removes and to sprout.
The budding seedling of normal bud grafting in season, 25 ~ 30d after bud grafting, the budding seedling that bud grafting becomes to live keeps first pair of true leaf and plucks the top, and uses scraper to excavate the axillalry bud of rough leaf, separates simultaneously and ties up.The budding seedling that stays the top to survive the winter; Temperature gos up and just can pinch operation when being stabilized in more than 15 ℃; It is aging or use sharp secateurs above first pair of true leaf, to cut off at 1cm place when just having sprouted to select stock phenology to be in the ceiling leaf, and the use scraper excavates the axillalry bud of rough leaf, separates simultaneously and ties up.Separate and want timely bud picking after tying up, promote scion to take out and send out.
5, use the promoter of sprouting to handle the bud sheet.
Respectively with 6-BA and GA
3Be dissolved in the 0.1mol/LHCl solution, being mixed with volumetric concentration is the 6-BA solution of 35～45mg/L and the GA of 10～15 mg/L
3Solution; Respectively TDZ and NAA are dissolved in that to be mixed with volumetric concentration in the 0.1mol/LNaOH solution be the TDZ solution of 75～85mg/L and the NAA solution of 25～35mg/L; Again they are mixed, use 0.1mol/LHCl solution or 0.1mol/LNaOH solution to regulate PH to 5.8～7.0 and promptly get the promoter of sprouting.The promoter of will sprouting earlier during use adding volume ratio is 0.02% Tween 80 or agricultural spreader-sticker, shakes up.The promoter of sprouting of on a small scale using absorbent cotton to dip in to prepare directly is coated with the bud picking sheet, is not gathered into water droplet degree of being with the moistening soup of whole bud sheet; Can the promoter of sprouting be diluted to a half strength in enormous quantities, carry out directed spraying after the use knapsack sprayer installs protective cover additional, squirt stock budding panel zone, avoid being sprayed onto blade and get final product.Handle in the 24h of back and do not water, meet rain in the outdoor cropping nursery stock 4h, need to stop using the soup coating of half strength after budding panel does not have ponding or filling spray once at rain.
6, strong seedling cultivation.
For making the robust growth of buddering, it is withered to prevent to budder back, manages after needing to strengthen cutting bar.(1) pinch one week of back, top application is become thoroughly decomposed fertilizer once, and is affixed by liquid manure, and every first quarter moon is used foliage fertilizer once, keeps compost moistening, to promote to budder sprouting; (2) bud picking regularly, erase stock cotyledon axillalry bud and other sprout tillers destroy apical dominance, let the nutrient centralizedly supply budder; (3) in time insect pests (mite) is harmful, and main sick worm (mite) evil that takes place is Eotetranychus sexmaculatus, rubber pair pearl lecanium, powdery mildew, anthracnose; Physiological disease mainly is a nutritional deficiency, and wherein iron deficiency and magnesium deficiency are comparatively common, can and handle according to symptom identification; (4) budling survives the winter, and budling need place in the greenhouse and cultivate autumn, newly takes out budlet ability safe overwintering in the normal time, and like consecutive low temperature weather, the processing of then need heating in the greenhouse guarantees mean temperature more than 15 ℃, and minimum temperature is more than 8 ℃.
Claims (1)
1. method that promotes that Para rubber tree budding seedling sprouts is characterized in that this method comprises:
1), the pre-treatment of pinching of budding seedling: pinch the last week at the budding seedling, the thin liquid manure that pouring is macerated system with chicken manure, peanut press pulp and phosphate fertilizer once, and the foliage-spray ammino acid liquid fertilizer is once;
2), stock is pinched and the stock bud removes and to sprout: the budding seedling of normal bud grafting in season, 25～30d after bud grafting, the budding seedling that bud grafting becomes to live keeps first pair of true leaf and plucks the top, and the use scraper excavates the axillalry bud of rough leaf, separates simultaneously and ties up; The budding seedling that stays the top to survive the winter; Temperature gos up and pinches operation when being stabilized in more than 15 ℃; It is aging or use sharp secateurs above first pair of true leaf, to cut off at the 1cm place when just having sprouted to select stock phenology to be in the ceiling leaf; And use scraper to excavate the axillalry bud of rough leaf, and separate simultaneously and tie up, separate and want timely bud picking after tying up;
3), use the promoter of sprouting to handle the bud sheet: respectively with 6-BA and GA
3Be dissolved in the 0.1mol/LHCl solution, being mixed with volumetric concentration is the 6-BA solution of 35～45mg/L and the GA of 10～15 mg/L
3Solution; Respectively TDZ and NAA are dissolved in that to be mixed with volumetric concentration in the 0.1mol/LNaOH solution be the TDZ solution of 75～85mg/L and the NAA solution of 25～35mg/L; Again they are mixed; Use 0.1mol/LHCl solution or 0.1mol/LNaOH solution to regulate PH to 5.8～7.0 and promptly get the promoter of sprouting; The promoter of will sprouting adds the Tween 80 or the agricultural spreader-sticker of volume ratio 0.02%, shakes up, and existing mixing at present used; Wherein, use absorbent cotton to dip in the promoter of sprouting on a small scale, directly be coated with the bud picking sheet, be not gathered into water droplet degree of being with the moistening soup of whole bud sheet; Can the promoter of sprouting be diluted to a half strength in enormous quantities, carry out directed spraying after the use knapsack sprayer installs protective cover additional, squirt stock budding panel zone, avoid being sprayed onto blade and get final product; Handle in the 24h of back and do not water, meet rain in the outdoor cropping nursery stock 4h, need to stop using the soup coating of half strength after budding panel does not have ponding or filling spray once at rain;
4), strong seedling cultivation: pinch one week of back, top application is become thoroughly decomposed fertilizer once, and is affixed by liquid manure, and every first quarter moon is used foliage fertilizer once, keeps compost moistening, to promote to budder sprouting; Bud picking regularly, erase stock cotyledon axillalry bud and other sprout tillers destroy apical dominance, let the nutrient centralizedly supply budder; Prevention and elimination of disease and pests in time, the main damage by disease and insect that takes place are the secondary pearl lecanium of Eotetranychus sexmaculatus, rubber, powdery mildew, anthracnose; Physiological disease mainly is a nutritional deficiency, and wherein iron deficiency and magnesium deficiency are comparatively common, can and handle according to symptom identification; Budling survives the winter, and budling need place in the greenhouse and cultivate autumn, newly takes out budlet ability safe overwintering in the normal time, and like consecutive low temperature weather, the processing of then need heating in the greenhouse guarantees mean temperature more than 15 ℃, and minimum temperature is more than 8 ℃.
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Cited By (9)
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| CN103503699A (en) * | 2013-07-08 | 2014-01-15 | 云南省热带作物科学研究所 | Reproduction method for rejuvenizing excellent mature individual plant of rubber tree |
| CN103524188A (en) * | 2013-10-22 | 2014-01-22 | 李桥旺 | Fertilizer for fruits or vegetables and preparation method thereof |
| CN103749177A (en) * | 2014-01-21 | 2014-04-30 | 中国热带农业科学院橡胶研究所 | Bud picking method for rubber tree bud grafted seedlings |
| CN103947448A (en) * | 2014-05-20 | 2014-07-30 | 中国热带农业科学院橡胶研究所 | Method for increasing budding and growing consistency of bud grafting seedlings of rubber seeds |
| CN104620924A (en) * | 2015-01-29 | 2015-05-20 | 云南农垦集团西盟橡胶有限责任公司 | Method for planting rubber trees |
| CN104871752A (en) * | 2014-02-28 | 2015-09-02 | 中国热带农业科学院橡胶研究所 | Explant pretreatment method before inducing and culturing multiple shoot of rubber tree nursery stock |
| CN105746282A (en) * | 2016-03-22 | 2016-07-13 | 中国热带农业科学院橡胶研究所 | Method for nursery multiplication as well as rejuvenation and replanting of rubber tree buds |
| CN106489651A (en) * | 2016-11-17 | 2017-03-15 | 云南省热带作物科学研究所 | A kind of method for culturing seedlings of fast culture rubber tree seedling |
| CN111386891A (en) * | 2020-04-20 | 2020-07-10 | 中国热带农业科学院橡胶研究所 | Bud picking-free method for rubber tree seedling bud grafting stock seedlings |
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Cited By (16)
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| CN103503699A (en) * | 2013-07-08 | 2014-01-15 | 云南省热带作物科学研究所 | Reproduction method for rejuvenizing excellent mature individual plant of rubber tree |
| CN103503699B (en) * | 2013-07-08 | 2015-05-13 | 云南省热带作物科学研究所 | Reproduction method for rejuvenizing excellent mature individual plant of rubber tree |
| CN103524188B (en) * | 2013-10-22 | 2015-03-25 | 李桥旺 | Fertilizer for fruits or vegetables and preparation method thereof |
| CN103524188A (en) * | 2013-10-22 | 2014-01-22 | 李桥旺 | Fertilizer for fruits or vegetables and preparation method thereof |
| CN103749177B (en) * | 2014-01-21 | 2015-10-28 | 中国热带农业科学院橡胶研究所 | A kind of Budding In Hevea seedling bud picking method |
| CN103749177A (en) * | 2014-01-21 | 2014-04-30 | 中国热带农业科学院橡胶研究所 | Bud picking method for rubber tree bud grafted seedlings |
| CN104871752A (en) * | 2014-02-28 | 2015-09-02 | 中国热带农业科学院橡胶研究所 | Explant pretreatment method before inducing and culturing multiple shoot of rubber tree nursery stock |
| CN104871752B (en) * | 2014-02-28 | 2017-07-11 | 中国热带农业科学院橡胶研究所 | A kind of explant preprocess method before rubber tree nursery stock inducing clumping bud culture |
| CN103947448A (en) * | 2014-05-20 | 2014-07-30 | 中国热带农业科学院橡胶研究所 | Method for increasing budding and growing consistency of bud grafting seedlings of rubber seeds |
| CN104620924A (en) * | 2015-01-29 | 2015-05-20 | 云南农垦集团西盟橡胶有限责任公司 | Method for planting rubber trees |
| CN104620924B (en) * | 2015-01-29 | 2017-02-22 | 云南农垦集团西盟橡胶有限责任公司 | Method for planting rubber trees |
| CN105746282A (en) * | 2016-03-22 | 2016-07-13 | 中国热带农业科学院橡胶研究所 | Method for nursery multiplication as well as rejuvenation and replanting of rubber tree buds |
| CN105746282B (en) * | 2016-03-22 | 2018-07-27 | 中国热带农业科学院橡胶研究所 | Rubber tree budling nursery proliferation and rejuvenation thereafter and method for changing |
| CN106489651A (en) * | 2016-11-17 | 2017-03-15 | 云南省热带作物科学研究所 | A kind of method for culturing seedlings of fast culture rubber tree seedling |
| CN111386891A (en) * | 2020-04-20 | 2020-07-10 | 中国热带农业科学院橡胶研究所 | Bud picking-free method for rubber tree seedling bud grafting stock seedlings |
| CN111386891B (en) * | 2020-04-20 | 2021-09-10 | 中国热带农业科学院橡胶研究所 | Bud picking-free method for rubber tree seedling bud grafting stock seedlings |
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