CN102627379B - Method for storing and exciting anaerobic ammonium oxidation sludge - Google Patents

Abstract

The invention relates to a method for storing and exciting anaerobic ammonium oxidation sludge. The method for storing the anaerobic ammonium oxidation sludge comprises the steps as follows: firstly, washing the anaerobic ammonium oxidation sludge needed to be stored by an inorganic salt solution, and placing in a storage container; secondly, adding the inorganic salt solution for adjusting the volatile suspended solid concentration of the anaerobic ammonium oxidation sludge to be 10-36 g/L and the pH value to be 7.5-8.0; thirdly, filling argon gas; and lastly, sealing the storage container, and storing in a refrigerator of 0-4 DEG C. The method for exciting the anaerobic ammonium oxidation sludge comprises the steps as follows: taking out the anaerobic ammonium oxidation sludge, washing by the inorganic salt solution, adding a nutrient solution, filling up distillation water into an excitation container, adjusting the pH value to be 7.0-7.5, filling the argon gas, vibrating for cultivation, sampling and measuring the concentrations of NH4+-N and NO2--N in the cultivation process, and adding a matrix concentration solution as needed. The method has the advantages of simple equipment requirements, higher activity retention rate, shorter excitation time and better excitation effect.

Description

A kind of preservation of Anammox mud and Activiation method

Technical field

The present invention relates to a kind of preservation and Activiation method of Anammox mud, belong to field of Environment Protection.

Background technology

Anammox is a kind of new and effective biological denitrification process, not only can save in the oxygenation energy consumption of nitration reaction in traditional denitrification process and anti-nitration reaction and need the external source organic electronic donor added, also improve nitration reaction and produce acid, denitrification produces alkali and all needs the situation neutralized.Reduce the demand of chemical reagent, prevented the secondary pollution that may occur.Since coming out, just be subject to showing great attention to.

Anaerobic ammonia oxidizing bacteria belongs to Planctomycetes, is chemosynthetic autotroph biology, poor growth.Therefore caused this technique long shortcoming start time, and anaerobic ammonia oxidizing bacteria to envrionment conditions for example, temperature, salinity, pH etc. are extremely responsive, and the easy unstability of technique is recovered slowly.

Therefore can carry out effective preservation to the anaerobic ammonia oxidizing bacteria of high-quality, and can be in the situation that needs, by certain technique means, the activity that activates anaerobic ammonia oxidizing bacteria is significant.Its inoculation source as new startup reactor can be able to be realized to the quick startup of Anammox, or, in the situation that the reactor unstability is added organism as external source, guarantee the process operation efficient stable.

Summary of the invention

The object of the present invention is to provide a kind of method of preservation and activation of Anammox mud.

The inventive method comprises method for preserving and the Activiation method of Anammox mud, wherein

The method for preserving of Anammox mud is specifically: at first will need inorganic salt solution for the Anammox mud of preservation to rinse 2～5 times, be placed in the preservation container, the volatile suspended sediment concentration that adds inorganic salt solution to adjust Anammox mud is 10～36g/L, with the NaOH solution of 1～2mol/L or the HCl solution of 1～2mol/L, the pH value of preservation liquid in container is adjusted to 7.5～8.0, then be filled with argon gas, the preservation container sealing is placed on to 0～4 ℃ of refrigerator preservation.

The Activiation method of Anammox mud is specifically: take out as required part Anammox mud in the preservation container, be placed in activation vessel, with inorganic salt solution, rinse 2～5 times, add the nutritive medium that is equivalent to activation vessel effective volume 15～20 ﹪, add distilled water that activation vessel is filled it up with, with the NaOH solution of 1～2mol/L or the HCl solution of 1～2mol/L, the pH value of liquid in activation vessel is adjusted to 7.0～7.5, then be filled with argon gas, activation vessel is placed in to the shaking culture case, cultivates under 30～35 ℃, 120～180rpm rotating speed; In culturing process every 10～12h sampling and measuring NH ₄ ⁺-N and NO ₂ ^--N concentration, as wherein a kind of concentration is less than or equal to 5mg/L, add corresponding NH ₄ ⁺-N matrix concentrated solution or NO ₂ ^--N matrix concentrated solution, the concentration of matrix concentrated solution is 1000～1500mg/L, 1.5～2 ﹪ that add-on is the activation vessel effective volume; After cultivating 48～120h, taking-up mud can come into operation.

The component of the inorganic salt solution in method for preserving and Activiation method and the concentration of each component are: the KH of 10～15mg/L ₂pO ₄, 5.6～8.4mg/L CaCl ₂2H ₂the MgSO of O, 300～450mg/L ₄7H ₂the KHCO of O, 1000～1500mg/L ₃;

The component of the nutritive medium described in Activiation method and the concentration of each component are: the NH of 100～150mg/L ₄ ⁺the NO of-N, 100～150mg/L ₂ ^-the KH of-N, 50～75mg/L ₂pO ₄, 28～42mg/L CaCl ₂2H ₂the MgSO of O, 1500～2250mg/L ₄7H ₂the KHCO of O, 5000～7500mg/L ₃, the EDTA of 125.5～187.5mg/L, the FeSO of 57.1～85.7mg/L ₄7H ₂the H of O, 0.0875～0.13mg/L ₃bO ₄, 6.19～9.28mg/L MnCl ₂4H ₂the CuSO of O, 1.56～2.34mg/L ₄5H ₂the ZnSO of O, 2.69～4.03mg/L ₄7H ₂the NiCl of O, 1.31～1.97mg/L ₂6H ₂the NaMoO of O, 1.38～2.06mg/L ₄2H ₂the CoCl of O, 1.5～2.25mg/L ₂6H ₂o.

The inventive method equipment requirements is simple, does not need strict process control, can realize the long-term preservation of Anammox mud, and activity preservation rate is higher; Activationary time is shorter simultaneously, and activation effect is good; Applied widely; Cost is lower.The method has solved the inoculation source problem of anaerobic ammonia oxidation process application, has a extensive future.

Embodiment

Embodiment 1.

The method for preserving of Anammox mud: at first will need the Anammox mud of preservation to rinse 2 times with inorganic salt solution, be placed in the preservation container, the volatile suspended sediment concentration that adds inorganic salt solution to adjust Anammox mud is 10g/L, with NaOH or the HCl solution of 1mol/L, the pH value of preservation liquid in container is adjusted to 7.5, then be filled with argon gas, the preservation container sealing is placed on to 0 ℃ of refrigerator preservation.

In preservation, after 1st month, its activity is 17.8mgN (gVSSh) ^-1, activity preservation rate is 82.8%, can think effectively preservation Anammox mud of the method.

The Activiation method of Anammox mud: take out as required part Anammox mud in the preservation container, be placed in activation vessel, with inorganic salt solution, rinse 2 times, add the nutritive medium that is equivalent to activation vessel effective volume 15 ﹪, add distilled water that activation vessel is filled it up with, with NaOH or the HCl solution of 1mol/L, the pH value of liquid in activation vessel is adjusted to 7.0, then is filled with argon gas; Activation vessel is placed in to the shaking culture case, cultivates under 30 ℃, 180rpm rotating speed; In culturing process every 12h sampling and measuring NH ₄ ⁺-N and NO ₂ ^--N concentration, as wherein a kind of concentration is less than or equal to 5mg/L, add the NH that corresponding concentration is 1000mg/L ₄ ⁺-N matrix concentrated solution or NO ₂ ^--N matrix concentrated solution, 2 ﹪ that add-on is the activation vessel effective volume; After cultivating 48h, taking-up mud can come into operation.

The component of above-mentioned inorganic salt solution and the concentration of each component are: the KH of 10mg/L ₂pO ₄, 8.4mg/L CaCl ₂2H ₂the MgSO of O, 300mg/L ₄7H ₂the KHCO of O, 1500mg/L ₃;

The component of above-mentioned nutritive medium and the concentration of each component are: the NH of 150mg/L ₄ ⁺the NO of-N, 150mg/L ₂ ^-the KH of-N, 50mg/L ₂pO ₄, 28mg/L CaCl ₂2H ₂the MgSO of O, 2250mg/L ₄7H ₂the KHCO of O, 7500mg/L ₃, the EDTA of 125.5mg/L, the FeSO of 57.1mg/L ₄7H ₂the H of O, 0.0875mg/L ₃bO ₄, 6.19mg/L MnCl ₂4H ₂the CuSO of O, 2.34mg/L ₄5H ₂the ZnSO of O, 4.03mg/L ₄7H ₂the NiCl of O, 1.97mg/L ₂6H ₂the NaMoO of O, 2.06mg/L ₄2H ₂the CoCl of O, 2.25mg/L ₂6H ₂o.

Experiment shows to process through the activation of 3 days, even the hungry preservation mud of 1 month, its activity can reach 19.5mgN (gVSSh) ^-1, return to 90.7% of the front activity of preservation.

Embodiment 2.

The method for preserving of Anammox mud: at first will need the Anammox mud of preservation to rinse 3 times with inorganic salt solution, be placed in the preservation container, the volatile suspended sediment concentration that adds inorganic salt solution to adjust Anammox mud is 20g/L, with NaOH or the HCl solution of 1.5mol/L, the pH value of preservation liquid in container is adjusted to 7.8, then be filled with argon gas, the preservation container sealing is placed on to 1 ℃ of refrigerator preservation.

In preservation, after 1st month, its activity is 18.1mgN (gVSSh) ^-1, activity preservation rate is 84.2%, can think effectively preservation Anammox mud of the method.

The Activiation method of Anammox mud: take out as required part Anammox mud in the preservation container, be placed in activation vessel, with inorganic salt solution, rinse 3 times, add the nutritive medium that is equivalent to activation vessel effective volume 20 ﹪, add distilled water that activation vessel is filled it up with, with NaOH or the HCl solution of 1.5mol/L, the pH value of liquid in activation vessel is adjusted to 7.2, then is filled with argon gas; Activation vessel is placed in to the shaking culture case, cultivates under 32 ℃, 150rpm rotating speed; In culturing process every 10h sampling and measuring NH ₄ ⁺-N and NO ₂ ^--N concentration, as wherein a kind of concentration is less than or equal to 5mg/L, add the NH that corresponding concentration is 1200mg/L ₄ ⁺-N matrix concentrated solution or NO ₂ ^--N matrix concentrated solution, 1.6 ﹪ that add-on is the activation vessel effective volume; After cultivating 60h, taking-up mud can come into operation.

The component of above-mentioned inorganic salt solution and the concentration of each component are: the KH of 12mg/L ₂pO ₄, 8.0mg/L CaCl ₂2H ₂the MgSO of O, 450mg/L ₄7H ₂the KHCO of O, 1200mg/L ₃;

The component of above-mentioned nutritive medium and the concentration of each component are: the NH of 100mg/L ₄ ⁺the NO of-N, 100mg/L ₂ ^-the KH of-N, 60mg/L ₂pO ₄, 35mg/L CaCl ₂2H ₂the MgSO of O, 1500mg/L ₄7H ₂the KHCO of O, 6000mg/L ₃, the EDTA of 151mg/L, the FeSO of 60.5mg/L ₄7H ₂the H of O, 0.1mg/L ₃bO ₄, 7.23mg/L MnCl ₂4H ₂the CuSO of O, 1.56mg/L ₄5H ₂the ZnSO of O, 2.69mg/L ₄7H ₂the NiCl of O, 1.31mg/L ₂6H ₂the NaMoO of O, 1.38mg/L ₄2H ₂the CoCl of O, 1.5mg/L ₂6H ₂o.

Experiment shows to process through the activation of 3 days, even the hungry preservation mud of 1 month, its activity can reach 19.3mgN (gVSSh) ^-1, return to 89.8% of the front activity of preservation.

Embodiment 3

The method for preserving of Anammox mud: at first will need the Anammox mud of preservation to rinse 4 times with inorganic salt solution, be placed in the preservation container, the volatile suspended sediment concentration that adds inorganic salt solution to adjust Anammox mud is 30g/L, with NaOH or the HCl solution of 2mol/L, the pH value of preservation liquid in container is adjusted to 8.0, then be filled with argon gas, the preservation container sealing is placed on to 4 ℃ of refrigerator preservations.

In preservation, after 1st month, its activity is 17.9mgN (gVSSh) ^-1, activity preservation rate is 83.3%, can think effectively preservation Anammox mud of the method.

The Activiation method of Anammox mud: take out as required part Anammox mud in the preservation container, be placed in activation vessel, with inorganic salt solution, rinse 4 times, add the nutritive medium that is equivalent to activation vessel effective volume 15 ﹪, add distilled water that activation vessel is filled it up with, with NaOH or the HCl solution of 2mol/L, the pH value of liquid in activation vessel is adjusted to 7.5, then is filled with argon gas; Activation vessel is placed in to the shaking culture case, cultivates under 35 ℃, 120rpm rotating speed; In culturing process every 11h sampling and measuring NH ₄ ⁺-N and NO ₂ ^--N concentration, as wherein a kind of concentration is less than or equal to 5mg/L, add the NH that corresponding concentration is 1500mg/L ₄ ⁺-N matrix concentrated solution or NO ₂ ^--N matrix concentrated solution, 1.5 ﹪ that add-on is the activation vessel effective volume; After cultivating 120h, taking-up mud can come into operation.

The component of above-mentioned inorganic salt solution and the concentration of each component are: the KH of 15mg/L ₂pO ₄, 5.6mg/L CaCl ₂2H ₂the MgSO of O, 400mg/L ₄7H ₂the KHCO of O, 1000mg/L ₃;

The component of above-mentioned nutritive medium and the concentration of each component are: the NH of 120mg/L ₄ ⁺the NO of-N, 120mg/L ₂ ^-the KH of-N, 75mg/L ₂pO ₄, 42mg/L CaCl ₂2H ₂the MgSO of O, 2000mg/L ₄7H ₂the KHCO of O, 5000mg/L ₃, the EDTA of 187.5mg/L, the FeSO of 85.7mg/L ₄7H ₂the H of O, 0.13mg/L ₃bO ₄, 9.28mg/L MnCl ₂4H ₂the CuSO of O, 2.04mg/L ₄5H ₂the ZnSO of O, 3.25mg/L ₄7H ₂the NiCl of O, 1.58mg/L ₂6H ₂the NaMoO of O, 1.65mg/L ₄2H ₂the CoCl of O, 2.0mg/L ₂6H ₂o.

Experiment shows to process through the activation of 3 days, even the hungry preservation mud of 1 month, its activity can reach 19.1mgN (gVSSh) ^-1, return to 88.9% of the front activity of preservation.

Embodiment 4

The method for preserving of Anammox mud: at first will need the Anammox mud of preservation to rinse 5 times with inorganic salt solution, be placed in the preservation container, the volatile suspended sediment concentration that adds inorganic salt solution to adjust Anammox mud is 36g/L, with NaOH solution or the HCl solution of 1.2mol/L, the pH value of preservation liquid in container is adjusted to 7.6, then be filled with argon gas, the preservation container sealing is placed on to 2 ℃ of refrigerator preservations.

In preservation, after 1st month, its activity is 18.4mgN (gVSSh) ^-1, activity preservation rate is 85.6%, can think effectively preservation Anammox mud of the method.

The Activiation method of Anammox mud: take out as required part Anammox mud in the preservation container, be placed in activation vessel, with inorganic salt solution, rinse 5 times, add the nutritive medium that is equivalent to activation vessel effective volume 18 ﹪, add distilled water that activation vessel is filled it up with, with NaOH or the HCl solution of 1.2mol/L, the pH value of liquid in activation vessel is adjusted to 7.3, then is filled with argon gas; Activation vessel is placed in to the shaking culture case, cultivates under 30 ℃, 150rpm rotating speed; In culturing process every 12h sampling and measuring NH ₄ ⁺-N and NO ₂ ^--N concentration, as wherein a kind of concentration is less than or equal to 5mg/L, add the NH that corresponding concentration is 1500mg/L ₄ ⁺-N matrix concentrated solution or NO ₂ ^--N matrix concentrated solution, 2 ﹪ that add-on is the activation vessel effective volume; After cultivating 96h, taking-up mud can come into operation.

The component of above-mentioned inorganic salt solution and the concentration of each component are: the KH of 13mg/L ₂pO ₄, 6.4mg/L CaCl ₂2H ₂the MgSO of O, 350mg/L ₄7H ₂the KHCO of O, 1300mg/L ₃;

The component of above-mentioned nutritive medium and the concentration of each component are: the NH of 130mg/L ₄ ⁺the NO of-N, 130mg/L ₂ ^-the KH of-N, 55mg/L ₂pO ₄, 30mg/L CaCl ₂2H ₂the MgSO of O, 1800mg/L ₄7H ₂the KHCO of O, 7000mg/L ₃, the EDTA of 165.5mg/L, the FeSO of 80.2mg/L ₄7H ₂the H of O, 0.125mg/L ₃bO ₄, 8.31mg/L MnCl ₂4H ₂the CuSO of O, 1.84mg/L ₄5H ₂the ZnSO of O, 3.53mg/L ₄7H ₂the NiCl of O, 1.45mg/L ₂6H ₂the NaMoO of O, 1.86mg/L ₄2H ₂the CoCl of O, 1.85mg/L ₂6H ₂o.

Experiment shows to process through the activation of 3 days, even the hungry preservation mud of 1 month, its activity can reach 19.3mgN (gVSSh) ^-1, return to 89.8% of the front activity of preservation.

Embodiment 5

The method for preserving of Anammox mud: at first will need the Anammox mud of preservation to rinse 3 times with inorganic salt solution, be placed in the preservation container, the volatile suspended sediment concentration that adds inorganic salt solution to adjust Anammox mud is 30g/L, with NaOH solution or the HCl solution of 1mol/L, the pH value of preservation liquid in container is adjusted to 7.0, then be filled with argon gas, the preservation container sealing is placed on to 3 ℃ of refrigerator preservations.

In preservation, after 1st month, its activity is 18.6mgN (gVSSh) ^-1, activity preservation rate is 86.5%.Therefore can think effectively preservation Anammox mud of the method.

The Activiation method of Anammox mud: take out as required part Anammox mud in the preservation container, be placed in activation vessel, with inorganic salt solution, rinse 3 times, add the nutritive medium that is equivalent to activation vessel effective volume 20 ﹪, add distilled water that activation vessel is filled it up with, with NaOH solution or the HCl solution of 1mol/L, the pH value of liquid in activation vessel is adjusted to 7.5, then is filled with argon gas; Activation vessel is placed in to the shaking culture case, cultivates under 35 ℃, 120rpm rotating speed; In culturing process every 10h sampling and measuring NH ₄ ⁺-N and NO ₂ ^--N concentration, as wherein a kind of concentration is less than or equal to 5mg/L, add the NH that corresponding concentration is 1500mg/L ₄ ⁺-N matrix concentrated solution or NO ₂ ^--N matrix concentrated solution, 1.8 ﹪ that add-on is the activation vessel effective volume; After cultivating 72h, taking-up mud can come into operation.

The component of above-mentioned inorganic salt solution and the concentration of each component are: the KH of 11mg/L ₂pO ₄, 8.4mg/L CaCl ₂2H ₂the MgSO of O, 400mg/L ₄7H ₂the KHCO of O, 1200mg/L ₃;

The component of above-mentioned nutritive medium and the concentration of each component are: the NH of 150mg/L ₄ ⁺the NO of-N, 150mg/L ₂ ^-the KH of-N, 50mg/L ₂pO ₄, 42mg/L CaCl ₂2H ₂the MgSO of O, 2000mg/L ₄7H ₂the KHCO of O, 5500mg/L ₃, the EDTA of 143.5mg/L, the FeSO of 57.1mg/L ₄7H ₂the H of O, 0.13mg/L ₃bO ₄, 6.19mg/L MnCl ₂4H ₂the CuSO of O, 1.56mg/L ₄5H ₂the ZnSO of O, 3.45mg/L ₄7H ₂the NiCl of O, 1.78mg/L ₂6H ₂the NaMoO of O, 1.66mg/L ₄2H ₂the CoCl of O, 1.65mg/L ₂6H ₂o.

Experiment shows to process through the activation of 3 days, even the hungry preservation mud of 1 month, its activity can reach 19.5mgN (gVSSh) ^-1, return to 90.7% of the front activity of preservation.

Claims (1)

1. the preservation of an Anammox mud and Activiation method comprise and it is characterized in that method for preserving and the Activiation method of Anammox mud:

The method for preserving of described Anammox mud is specifically: at first will need inorganic salt solution for the Anammox mud of preservation to rinse 2～5 times, be placed in the preservation container, the volatile suspended sediment concentration that adds inorganic salt solution to adjust Anammox mud is 10～36g/L, with the NaOH solution of 1～2mol/L or the HCl solution of 1～2mol/L, the pH value of preservation liquid in container is adjusted to 7.5～8.0, then be filled with argon gas, the preservation container sealing is placed on to 0～4 ℃ of refrigerator preservation;

The Activiation method of described Anammox mud is specifically: take out as required part Anammox mud in the preservation container, be placed in activation vessel, with inorganic salt solution, rinse 2～5 times, add the nutritive medium that is equivalent to activation vessel effective volume 15～20 ﹪, add distilled water that activation vessel is filled it up with, with the NaOH solution of 1～2mol/L or the HCl solution of 1～2mol/L, the pH value of liquid in activation vessel is adjusted to 7.0～7.5, then be filled with argon gas, activation vessel is placed in to the shaking culture case, cultivates under 30～35 ℃, 120～180rpm rotating speed; In culturing process every 10～12h sampling and measuring NH ₄ ⁺-N and NO ₂ ^--N concentration, as wherein a kind of concentration is less than or equal to 5mg/L, add corresponding NH ₄ ⁺-N matrix concentrated solution or NO ₂ ^--N matrix concentrated solution, the concentration of matrix concentrated solution is 1000～1500mg/L, 1.5～2 ﹪ that add-on is the activation vessel effective volume; After cultivating 48～120h, taking-up mud can come into operation;

The component of the inorganic salt solution described in method for preserving and Activiation method and the concentration of each component are: the KH of 10～15mg/L ₂pO ₄, 5.6～8.4mg/L CaCl ₂2H ₂the MgSO of O, 300～450mg/L ₄7H ₂the KHCO of O, 1000～1500mg/L ₃;

The component of the nutritive medium described in Activiation method and the concentration of each component are: the NH of 100～150mg/L ₄ ⁺the NO of-N, 100～150mg/L ₂ ^-the KH of-N, 50～75mg/L ₂pO ₄, 28～42mg/L CaCl ₂2H ₂the MgSO of O, 1500～2250mg/L ₄7H ₂the KHCO of O, 5000～7500mg/L ₃, the EDTA of 125.5～187.5mg/L, the FeSO of 57.1～85.7mg/L ₄7H ₂the H of O, 0.0875～0.13mg/L ₃bO ₄, 6.19～9.28mg/L MnCl ₂4H ₂the CuSO of O, 1.56～2.34mg/L ₄5H ₂the ZnSO of O, 2.69～4.03mg/L ₄7H ₂the NiCl of O, 1.31～1.97mg/L ₂6H ₂the NaMoO of O, 1.38～2.06mg/L ₄2H ₂the CoCl of O, 1.5～2.25mg/L ₂6H ₂o.