CN102627379A - Method for storing and exciting anaerobic ammonium oxidation sludge - Google Patents
Method for storing and exciting anaerobic ammonium oxidation sludge Download PDFInfo
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- CN102627379A CN102627379A CN2012100837233A CN201210083723A CN102627379A CN 102627379 A CN102627379 A CN 102627379A CN 2012100837233 A CN2012100837233 A CN 2012100837233A CN 201210083723 A CN201210083723 A CN 201210083723A CN 102627379 A CN102627379 A CN 102627379A
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- salt solution
- activation vessel
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- 238000000034 method Methods 0.000 title claims abstract description 53
- 230000003647 oxidation Effects 0.000 title abstract description 8
- 238000007254 oxidation reaction Methods 0.000 title abstract description 8
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 title abstract 7
- 239000010802 sludge Substances 0.000 title abstract 7
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 claims abstract description 32
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 31
- 239000012266 salt solution Substances 0.000 claims abstract description 31
- 239000000243 solution Substances 0.000 claims abstract description 23
- 239000011159 matrix material Substances 0.000 claims abstract description 17
- 229910052786 argon Inorganic materials 0.000 claims abstract description 16
- 239000007789 gas Substances 0.000 claims abstract description 16
- 238000005070 sampling Methods 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000004321 preservation Methods 0.000 claims description 74
- 230000004913 activation Effects 0.000 claims description 48
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 42
- 239000007788 liquid Substances 0.000 claims description 30
- 238000011010 flushing procedure Methods 0.000 claims description 14
- 230000000050 nutritive effect Effects 0.000 claims description 14
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 7
- 239000013049 sediment Substances 0.000 claims description 7
- 230000000694 effects Effects 0.000 abstract description 20
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 3
- 230000005284 excitation Effects 0.000 abstract 3
- 238000005406 washing Methods 0.000 abstract 2
- 238000004821 distillation Methods 0.000 abstract 1
- 230000014759 maintenance of location Effects 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 241001453382 Nitrosomonadales Species 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000006396 nitration reaction Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 2
- 241001314279 Zoopagales Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for storing and exciting anaerobic ammonium oxidation sludge. The method for storing the anaerobic ammonium oxidation sludge comprises the steps as follows: firstly, washing the anaerobic ammonium oxidation sludge needed to be stored by an inorganic salt solution, and placing in a storage container; secondly, adding the inorganic salt solution for adjusting the volatile suspended solid concentration of the anaerobic ammonium oxidation sludge to be 10-36 g/L and the pH value to be 7.5-8.0; thirdly, filling argon gas; and lastly, sealing the storage container, and storing in a refrigerator of 0-4 DEG C. The method for exciting the anaerobic ammonium oxidation sludge comprises the steps as follows: taking out the anaerobic ammonium oxidation sludge, washing by the inorganic salt solution, adding a nutrient solution, filling up distillation water into an excitation container, adjusting the pH value to be 7.0-7.5, filling the argon gas, vibrating for cultivation, sampling and measuring the concentrations of NH4+-N and NO2--N in the cultivation process, and adding a matrix concentration solution as needed. The method has the advantages of simple equipment requirements, higher activity retention rate, shorter excitation time and better excitation effect.
Description
Technical field
The present invention relates to a kind of preservation and Activiation method of Anammox mud, belong to the environmental protection field.
Background technology
Anammox is a kind of new and effective biological denitrification process; Not only can practice thrift and need the external source organic electronic donor that adds in oxygenation energy consumption and the anti-nitration reaction of nitration reaction in traditional denitrification process; Also improve nitration reaction and produce acid, denitrification produces alkali and all needs the neutral situation.Reduce the demand of chemical reagent, prevented the secondary pollution that possibly occur.Since coming out, just received and having shown great attention to.
Anaerobic ammonia oxidizing bacteria belongs to floating mustiness Zoopagales, is chemosynthetic autotroph biology, poor growth.Therefore caused this technology long shortcoming start time, and anaerobic ammonia oxidizing bacteria to envrionment conditions for example, temperature, salinity, pH etc. are unusual responsive, and the easy unstability of technology is recovered slowly.
Therefore can carry out effective preservation to the fine anaerobic ammonia oxidizing bacteria, and can be under the situation of needs, through certain technique means, the activity that activates anaerobic ammonia oxidizing bacteria is significant.Can it can be realized the quick startup of Anammox as the new inoculation source that starts reactor drum, or under the situation of reactor drum unstability, add organism, guarantee technology operation efficient stable as external source.
Summary of the invention
The object of the present invention is to provide a kind of preservation and activated method of Anammox mud.
The inventive method comprises the method for preserving and the Activiation method of Anammox mud, wherein
The method for preserving of Anammox mud is specifically: the Anammox mud that at first will need preservation is with inorganic salt solution flushing 2～5 times; Place in the preservation container; The volatile suspended sediment concentration that adds inorganic salt solution adjustment Anammox mud is 10～36 g/L; With the HCl solution of the NaOH solution of 1～2mol/L or 1～2mol/L the pH value of preservation liquid in container is transferred to 7.5～8.0, charge into argon gas then, the preservation container sealing is placed on 0～4 ℃ of refrigerator preservation.
The Activiation method of Anammox mud is specifically: by taking out part Anammox mud in the preservation container as required; Place in the activation vessel; With inorganic salt solution flushing 2～5 times, add the nutritive medium that is equivalent to activation vessel effective volume 15～20 ﹪, add zero(ppm) water activation vessel is filled it up with; With the HCl solution of the NaOH solution of 1～2mol/L or 1～2mol/L the pH value of liquid in the activation vessel is transferred to 7.0～7.5; Charge into argon gas then, activation vessel is placed the shaking culture case, under 30～35 ℃, 120～180 rpm/min rotating speeds, cultivate; Every in the culturing process at a distance from 10～12h sampling and measuring NH
4 +-N and NO
2 --N concentration smaller or equal to 5mg/L, then adds corresponding NH like wherein a kind of concentration
4 +-N matrix liquid concentrator or NO
2 --N matrix liquid concentrator, the concentration of matrix liquid concentrator are 1000～1500mg/L, and add-on is 1.5～2 ﹪ of activation vessel effective volume; Taking-up mud can come into operation after cultivating 48～120h.
The component of the inorganic salt solution in method for preserving and the Activiation method and each component concentrations are: the KH of 10～15mg/L
2PO
4, 5.6～8.4 mg/L CaCl
22H
2The MgSO of O, 300～450 mg/L
47H
2The KHCO of O, 1000～1500mg/L
3
The component of the nutritive medium described in the Activiation method and each component concentrations are: the NH of 100～150mg/L
4 +The NO of-N, 100～150mg/L
2 -The KH of-N, 50～75mg/L
2PO
4, 28～42mg/L CaCl
22H
2The MgSO of O, 1500～2250 mg/L
47H
2The KHCO of O, 5000～7500mg/L
3, the EDTA of 125.5～187.5mg/L, the FeSO of 57.1～85.7mg/L
47H
2The H of O, 0.0875～0.13mg/L
3BO
4, 6.19～9.28mg/L MnCl
24H
2The CuSO of O, 1.56～2.34mg/L
45H
2The ZnSO of O, 2.69～4.03mg/L
47H
2The NiCl of O, 1.31～1.97mg/L
26H
2The NaMoO of O, 1.38～2.06mg/L
42H
2The CoCl of O, 1.5～2.25mg/L
26H
2O.
The inventive method equipment requirements is simple, does not need strict process control, can realize the long-term preservation of Anammox mud, and activity preservation rate is higher; Activationary time is shorter simultaneously, and activation effect is good; Applied widely; Cost is lower.This method has solved the inoculation source problem that anaerobic ammonia oxidation process is used, and has a extensive future.
Embodiment
Embodiment 1.
The method for preserving of Anammox mud: the Anammox mud that at first will need preservation is with inorganic salt solution flushing 2 times; Place in the preservation container; The volatile suspended sediment concentration that adds inorganic salt solution adjustment Anammox mud is 10 g/L; With the NaOH of 1mol/L or HCl solution the pH value of preservation liquid in container is transferred to 7.5, charge into argon gas then, the preservation container sealing is placed on 0 ℃ of refrigerator preservation.
After 1st month, its activity is 17.8mgN (gVSSh) in preservation
-1, activity preservation rate is 82.8%, can think effectively preservation Anammox mud of this method.
The Activiation method of Anammox mud: by taking out part Anammox mud in the preservation container as required; Place in the activation vessel; With inorganic salt solution flushing 2 times, add the nutritive medium that is equivalent to activation vessel effective volume 15 ﹪, add zero(ppm) water activation vessel is filled it up with; With the NaOH of 1mol/L or HCl solution the pH value of liquid in the activation vessel is transferred to 7.0, charge into argon gas then; Activation vessel is placed the shaking culture case, under 30 ℃, 180 rpm/min rotating speeds, cultivate; Every in the culturing process at a distance from 12h sampling and measuring NH
4 +-N and NO
2 --N concentration, smaller or equal to 5mg/L, then adding corresponding concentration is the NH of 1000mg/L like wherein a kind of concentration
4 +-N matrix liquid concentrator or NO
2 --N matrix liquid concentrator, add-on are 2 ﹪ of activation vessel effective volume; Taking-up mud can come into operation after cultivating 48h.
The component of above-mentioned inorganic salt solution and each component concentrations are: the KH of 10mg/L
2PO
4, 8.4 mg/L CaCl
22H
2The MgSO of O, 300mg/L
47H
2The KHCO of O, 1500mg/L
3
The component of above-mentioned nutritive medium and each component concentrations are: the NH of 150mg/L
4 +The NO of-N, 150mg/L
2 -The KH of-N, 50mg/L
2PO
4, 28mg/L CaCl
22H
2The MgSO of O, 2250 mg/L
47H
2The KHCO of O, 7500mg/L
3, the EDTA of 125.5mg/L, the FeSO of 57.1mg/L
47H
2The H of O, 0.0875mg/L
3BO
4, 6.19mg/L MnCl
24H
2The CuSO of O, 2.34mg/L
45H
2The ZnSO of O, 4.03mg/L
47H
2The NiCl of O, 1.97mg/L
26H
2The NaMoO of O, 2.06mg/L
42H
2The CoCl of O, 2.25mg/L
26H
2O.
Experiment shows through 3 days activation to be handled, even 1 month mud of hungry preservation, its activity can reach 19.5 mgN (gVSSh)
-1, return to before the preservation active 90.7%.
Embodiment 2.
The method for preserving of Anammox mud: the Anammox mud that at first will need preservation is with inorganic salt solution flushing 3 times; Place in the preservation container; The volatile suspended sediment concentration that adds inorganic salt solution adjustment Anammox mud is 20 g/L; With the NaOH of 1.5mol/L or HCl solution the pH value of preservation liquid in container is transferred to 7.8, charge into argon gas then, the preservation container sealing is placed on 1 ℃ of refrigerator preservation.
After 1st month, its activity is 18.1mgN (gVSSh) in preservation
-1, activity preservation rate is 84.2%, can think effectively preservation Anammox mud of this method.
The Activiation method of Anammox mud: by taking out part Anammox mud in the preservation container as required; Place in the activation vessel; With inorganic salt solution flushing 3 times, add the nutritive medium that is equivalent to activation vessel effective volume 20 ﹪, add zero(ppm) water activation vessel is filled it up with; With the NaOH of 1.5mol/L or HCl solution the pH value of liquid in the activation vessel is transferred to 7.2, charge into argon gas then; Activation vessel is placed the shaking culture case, under 32 ℃, 150 rpm/min rotating speeds, cultivate; Every in the culturing process at a distance from 10h sampling and measuring NH
4 +-N and NO
2 --N concentration, smaller or equal to 5mg/L, then adding corresponding concentration is the NH of 1200mg/L like wherein a kind of concentration
4 +-N matrix liquid concentrator or NO
2 --N matrix liquid concentrator, add-on are 1.6 ﹪ of activation vessel effective volume; Taking-up mud can come into operation after cultivating 60h.
The component of above-mentioned inorganic salt solution and each component concentrations are: the KH of 12mg/L
2PO
4, 8.0 mg/L CaCl
22H
2The MgSO of O, 450mg/L
47H
2The KHCO of O, 1200mg/L
3
The component of above-mentioned nutritive medium and each component concentrations are: the NH of 100mg/L
4 +The NO of-N, 100mg/L
2 -The KH of-N, 60mg/L
2PO
4, 35mg/L CaCl
22H
2The MgSO of O, 1500 mg/L
47H
2The KHCO of O, 6000mg/L
3, the EDTA of 151mg/L, the FeSO of 60.5mg/L
47H
2The H of O, 0.1mg/L
3BO
4, 7.23mg/L MnCl
24H
2The CuSO of O, 1.56mg/L
45H
2The ZnSO of O, 2.69mg/L
47H
2The NiCl of O, 1.31mg/L
26H
2The NaMoO of O, 1.38mg/L
42H
2The CoCl of O, 1.5mg/L
26H
2O.
Experiment shows through 3 days activation to be handled, even 1 month mud of hungry preservation, its activity can reach 19.3 mgN (gVSSh)
-1, return to before the preservation active 89.8%.
Embodiment 3
The method for preserving of Anammox mud: the Anammox mud that at first will need preservation is with inorganic salt solution flushing 4 times; Place in the preservation container; The volatile suspended sediment concentration that adds inorganic salt solution adjustment Anammox mud is 30g/L; With the NaOH of 2mol/L or HCl solution the pH value of preservation liquid in container is transferred to 8.0, charge into argon gas then, the preservation container sealing is placed on 4 ℃ of refrigerator preservations.
After 1st month, its activity is 17.9mgN (gVSSh) in preservation
-1, activity preservation rate is 83.3%, can think effectively preservation Anammox mud of this method.
The Activiation method of Anammox mud: by taking out part Anammox mud in the preservation container as required; Place in the activation vessel; With inorganic salt solution flushing 4 times, add the nutritive medium that is equivalent to activation vessel effective volume 15 ﹪, add zero(ppm) water activation vessel is filled it up with; With the NaOH of 2mol/L or HCl solution the pH value of liquid in the activation vessel is transferred to 7.5, charge into argon gas then; Activation vessel is placed the shaking culture case, under 35 ℃, 120 rpm/min rotating speeds, cultivate; Every in the culturing process at a distance from 11h sampling and measuring NH
4 +-N and NO
2 --N concentration, smaller or equal to 5mg/L, then adding corresponding concentration is the NH of 1500mg/L like wherein a kind of concentration
4 +-N matrix liquid concentrator or NO
2 --N matrix liquid concentrator, add-on are 1.5 ﹪ of activation vessel effective volume; Taking-up mud can come into operation after cultivating 120h.
The component of above-mentioned inorganic salt solution and each component concentrations are: the KH of 15mg/L
2PO
4, 5.6mg/L CaCl
22H
2The MgSO of O, 400mg/L
47H
2The KHCO of O, 1000mg/L
3
The component of above-mentioned nutritive medium and each component concentrations are: the NH of 120mg/L
4 +The NO of-N, 120mg/L
2 -The KH of-N, 75mg/L
2PO
4, 42mg/L CaCl
22H
2The MgSO of O, 2000 mg/L
47H
2The KHCO of O, 5000mg/L
3, the EDTA of 187.5mg/L, the FeSO of 85.7mg/L
47H
2The H of O, 0.13mg/L
3BO
4, 9.28mg/L MnCl
24H
2The CuSO of O, 2.04mg/L
45H
2The ZnSO of O, 3.25mg/L
47H
2The NiCl of O, 1.58mg/L
26H
2The NaMoO of O, 1.65mg/L
42H
2The CoCl of O, 2.0mg/L
26H
2O.
Experiment shows through 3 days activation to be handled, even 1 month mud of hungry preservation, its activity can reach 19.1 mgN (gVSSh)
-1, return to before the preservation active 88.9%.
Embodiment 4
The method for preserving of Anammox mud: the Anammox mud that at first will need preservation is with inorganic salt solution flushing 5 times; Place in the preservation container; The volatile suspended sediment concentration that adds inorganic salt solution adjustment Anammox mud is 36g/L; With the NaOH solution of 1.2mol/L or HCl solution the pH value of preservation liquid in container is transferred to 7.6, charge into argon gas then, the preservation container sealing is placed on 2 ℃ of refrigerator preservations.
After 1st month, its activity is 18.4mgN (gVSSh) in preservation
-1, activity preservation rate is 85.6%, can think effectively preservation Anammox mud of this method.
The Activiation method of Anammox mud: by taking out part Anammox mud in the preservation container as required; Place in the activation vessel; With inorganic salt solution flushing 5 times, add the nutritive medium that is equivalent to activation vessel effective volume 18 ﹪, add zero(ppm) water activation vessel is filled it up with; With the NaOH of 1.2mol/L or HCl solution the pH value of liquid in the activation vessel is transferred to 7.3, charge into argon gas then; Activation vessel is placed the shaking culture case, under 30 ℃, 150 rpm/min rotating speeds, cultivate; Every in the culturing process at a distance from 12h sampling and measuring NH
4 +-N and NO
2 --N concentration, smaller or equal to 5mg/L, then adding corresponding concentration is the NH of 1500mg/L like wherein a kind of concentration
4 +-N matrix liquid concentrator or NO
2 --N matrix liquid concentrator, add-on are 2 ﹪ of activation vessel effective volume; Taking-up mud can come into operation after cultivating 96h.
The component of above-mentioned inorganic salt solution and each component concentrations are: the KH of 13mg/L
2PO
4, 6.4 mg/L CaCl
22H
2The MgSO of O, 350mg/L
47H
2The KHCO of O, 1300mg/L
3
The component of above-mentioned nutritive medium and each component concentrations are: the NH of 130mg/L
4 +The NO of-N, 130mg/L
2 -The KH of-N, 55mg/L
2PO
4, 30mg/L CaCl
22H
2The MgSO of O, 1800 mg/L
47H
2The KHCO of O, 7000mg/L
3, the EDTA of 165.5mg/L, the FeSO of 80.2mg/L
47H
2The H of O, 0.125mg/L
3BO
4, 8.31mg/L MnCl
24H
2The CuSO of O, 1.84mg/L
45H
2The ZnSO of O, 3.53mg/L
47H
2The NiCl of O, 1.45mg/L
26H
2The NaMoO of O, 1.86mg/L
42H
2The CoCl of O, 1.85mg/L
26H
2O.
Experiment shows through 3 days activation to be handled, even 1 month mud of hungry preservation, its activity can reach 19.3 mgN (gVSSh)
-1, return to before the preservation active 89.8%.
Embodiment 5
The method for preserving of Anammox mud: the Anammox mud that at first will need preservation is with inorganic salt solution flushing 3 times; Place in the preservation container; The volatile suspended sediment concentration that adds inorganic salt solution adjustment Anammox mud is 30g/L; With the NaOH solution of 1mol/L or HCl solution the pH value of preservation liquid in container is transferred to 7.0, charge into argon gas then, the preservation container sealing is placed on 3 ℃ of refrigerator preservations.
After 1st month, its activity is 18.6mgN (gVSSh) in preservation
-1, activity preservation rate is 86.5%.Therefore can think effectively preservation Anammox mud of this method.
The Activiation method of Anammox mud: by taking out part Anammox mud in the preservation container as required; Place in the activation vessel; With inorganic salt solution flushing 3 times, add the nutritive medium that is equivalent to activation vessel effective volume 20 ﹪, add zero(ppm) water activation vessel is filled it up with; With the NaOH solution of 1mol/L or HCl solution the pH value of liquid in the activation vessel is transferred to 7.5, charge into argon gas then; Activation vessel is placed the shaking culture case, under 35 ℃, 120 rpm/min rotating speeds, cultivate; Every in the culturing process at a distance from 10h sampling and measuring NH
4 +-N and NO
2 --N concentration, smaller or equal to 5mg/L, then adding corresponding concentration is the NH of 1500mg/L like wherein a kind of concentration
4 +-N matrix liquid concentrator or NO
2 --N matrix liquid concentrator, add-on are 1.8 ﹪ of activation vessel effective volume; Taking-up mud can come into operation after cultivating 72h.
The component of above-mentioned inorganic salt solution and each component concentrations are: the KH of 11mg/L
2PO
4, 8.4 mg/L CaCl
22H
2The MgSO of O, 400mg/L
47H
2The KHCO of O, 1200mg/L
3
The component of above-mentioned nutritive medium and each component concentrations are: the NH of 150mg/L
4 +The NO of-N, 150mg/L
2 -The KH of-N, 50mg/L
2PO
4, 42mg/L CaCl
22H
2The MgSO of O, 2000 mg/L
47H
2The KHCO of O, 5500mg/L
3, the EDTA of 143.5mg/L, the FeSO of 57.1mg/L
47H
2The H of O, 0.13mg/L
3BO
4, 6.19mg/L MnCl
24H
2The CuSO of O, 1.56mg/L
45H
2The ZnSO of O, 3.45mg/L
47H
2The NiCl of O, 1.78mg/L
26H
2The NaMoO of O, 1.66mg/L
42H
2The CoCl of O, 1.65mg/L
26H
2O.
Experiment shows through 3 days activation to be handled, even 1 month mud of hungry preservation, its activity can reach 19.5 mgN (gVSSh)
-1, return to before the preservation active 90.7%.
Claims (1)
1. the preservation of an Anammox mud and Activiation method comprise it is characterized in that the method for preserving and the Activiation method of Anammox mud:
The method for preserving of described Anammox mud is specifically: the Anammox mud that at first will need preservation is with inorganic salt solution flushing 2～5 times; Place in the preservation container; The volatile suspended sediment concentration that adds inorganic salt solution adjustment Anammox mud is 10～36 g/L; With the HCl solution of the NaOH solution of 1～2mol/L or 1～2mol/L the pH value of preservation liquid in container is transferred to 7.5～8.0; Charge into argon gas then, the preservation container sealing is placed on 0～4 ℃ of refrigerator preservation;
The Activiation method of described Anammox mud is specifically: by taking out part Anammox mud in the preservation container as required; Place in the activation vessel; With inorganic salt solution flushing 2～5 times, add the nutritive medium that is equivalent to activation vessel effective volume 15～20 ﹪, add zero(ppm) water activation vessel is filled it up with; With the HCl solution of the NaOH solution of 1～2mol/L or 1～2mol/L the pH value of liquid in the activation vessel is transferred to 7.0～7.5; Charge into argon gas then, activation vessel is placed the shaking culture case, under 30～35 ℃, 120～180 rpm/min rotating speeds, cultivate; Every in the culturing process at a distance from 10～12h sampling and measuring NH
4 +-N and NO
2 --N concentration smaller or equal to 5mg/L, then adds corresponding NH like wherein a kind of concentration
4 +-N matrix liquid concentrator or NO
2 --N matrix liquid concentrator, the concentration of matrix liquid concentrator are 1000～1500mg/L, and add-on is 1.5～2 ﹪ of activation vessel effective volume; Taking-up mud can come into operation after cultivating 48～120h;
The component of the inorganic salt solution described in method for preserving and the Activiation method and each component concentrations are: the KH of 10～15mg/L
2PO
4, 5.6～8.4 mg/L CaCl
22H
2The MgSO of O, 300～450 mg/L
47H
2The KHCO of O, 1000～1500mg/L
3
The component of the nutritive medium described in the Activiation method and each component concentrations are: the NH of 100～150mg/L
4 +The NO of-N, 100～150mg/L
2 -The KH of-N, 50～75mg/L
2PO
4, 28～42mg/L CaCl
22H
2The MgSO of O, 1500～2250 mg/L
47H
2The KHCO of O, 5000～7500mg/L
3, the EDTA of 125.5～187.5mg/L, the FeSO of 57.1～85.7mg/L
47H
2The H of O, 0.0875～0.13mg/L
3BO
4, 6.19～9.28mg/L MnCl
24H
2The CuSO of O, 1.56～2.34mg/L
45H
2The ZnSO of O, 2.69～4.03mg/L
47H
2The NiCl of O, 1.31～1.97mg/L
26H
2The NaMoO of O, 1.38～2.06mg/L
42H
2The CoCl of O, 1.5～2.25mg/L
26H
2O.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210083723 CN102627379B (en) | 2012-03-27 | 2012-03-27 | Method for storing and exciting anaerobic ammonium oxidation sludge |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210083723 CN102627379B (en) | 2012-03-27 | 2012-03-27 | Method for storing and exciting anaerobic ammonium oxidation sludge |
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| CN102627379B CN102627379B (en) | 2013-12-25 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103755109A (en) * | 2013-12-29 | 2014-04-30 | 杭州师范大学 | Storage and reactivation methods of anaerobic ammonia oxidation granule sludge |
| CN110129203A (en) * | 2019-04-23 | 2019-08-16 | 杭州师范大学 | A kind of preservation and restoration methods of Anammox strain |
| CN110255710A (en) * | 2019-06-14 | 2019-09-20 | 杭州师范大学 | A kind of tandem type anaerobic ammonia oxidation process for high-ammonia wastewater room temperature denitrogenation |
| CN111170451A (en) * | 2020-01-20 | 2020-05-19 | 杭州师范大学 | Method for rapidly improving activity of anaerobic ammonium oxidation sludge in short period |
| CN116694502A (en) * | 2023-03-13 | 2023-09-05 | 浙江大学 | Resuscitating method for anaerobic ammonia oxidation granular sludge after long-term low-temperature preservation |
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| CN101831392A (en) * | 2009-03-10 | 2010-09-15 | 中国科学院成都生物研究所 | Autotrophic and allotrophic symbiosis ammonia oxidation bacterial agent as well as culture method and application thereof |
| CN102061281A (en) * | 2010-11-30 | 2011-05-18 | 哈尔滨工业大学 | Method for enriching and culturing anaerobic ammonia oxidizing bacteria by using membrane bioreactor |
| CN102190371A (en) * | 2010-03-18 | 2011-09-21 | 华东师范大学 | Breeding method for anaerobic ammonium oxidation granular sludge |
| CN102336505A (en) * | 2011-07-18 | 2012-02-01 | 杭州师范大学 | Preservation method for anaerobic ammonium oxidation granular sludge |
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| CN101831392A (en) * | 2009-03-10 | 2010-09-15 | 中国科学院成都生物研究所 | Autotrophic and allotrophic symbiosis ammonia oxidation bacterial agent as well as culture method and application thereof |
| CN102190371A (en) * | 2010-03-18 | 2011-09-21 | 华东师范大学 | Breeding method for anaerobic ammonium oxidation granular sludge |
| CN102061281A (en) * | 2010-11-30 | 2011-05-18 | 哈尔滨工业大学 | Method for enriching and culturing anaerobic ammonia oxidizing bacteria by using membrane bioreactor |
| CN102336505A (en) * | 2011-07-18 | 2012-02-01 | 杭州师范大学 | Preservation method for anaerobic ammonium oxidation granular sludge |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103755109A (en) * | 2013-12-29 | 2014-04-30 | 杭州师范大学 | Storage and reactivation methods of anaerobic ammonia oxidation granule sludge |
| CN103755109B (en) * | 2013-12-29 | 2015-04-22 | 杭州师范大学 | Storage and reactivation methods of anaerobic ammonia oxidation granule sludge |
| CN110129203A (en) * | 2019-04-23 | 2019-08-16 | 杭州师范大学 | A kind of preservation and restoration methods of Anammox strain |
| CN110255710A (en) * | 2019-06-14 | 2019-09-20 | 杭州师范大学 | A kind of tandem type anaerobic ammonia oxidation process for high-ammonia wastewater room temperature denitrogenation |
| CN111170451A (en) * | 2020-01-20 | 2020-05-19 | 杭州师范大学 | Method for rapidly improving activity of anaerobic ammonium oxidation sludge in short period |
| CN116694502A (en) * | 2023-03-13 | 2023-09-05 | 浙江大学 | Resuscitating method for anaerobic ammonia oxidation granular sludge after long-term low-temperature preservation |
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