CN102061281A - Method for enriching and culturing anaerobic ammonia oxidizing bacteria by using membrane bioreactor - Google Patents
Method for enriching and culturing anaerobic ammonia oxidizing bacteria by using membrane bioreactor Download PDFInfo
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Abstract
The invention discloses a method for enriching and culturing anaerobic ammonia oxidizing bacteria by using a membrane bioreactor, relating to a method for enriching and culturing anaerobic ammonia oxidizing bacteria and solving the problems of long enriching time and low enriching efficiency of the anaerobic ammonia oxidizing bacteria in the traditional anaerobic ammonia oxidation process. The method for enriching and culturing the anaerobic ammonia oxidizing bacteria by using the membrane bioreactor as an enriching reactor comprises the steps of: inoculating activated sludge of a sewage plant in the membrane bioreactor; and 2, enriching and culturing after inoculating. Compared with the traditional enriching method, the method for enriching and culturing the anaerobic ammonia oxidizing bacteria by using the membrane bioreactor realizes rapid enrichment of the anaerobic ammonia oxidizing bacteria, ensures that the enriching efficiency of the anaerobic ammonia oxidizing bacteria is improved by 80-95 percent, and can be widely applied to the biological denitrification technical field.
Description
Technical field
The present invention relates to a kind of method of enrichment culture anaerobic ammonium oxidizing bacteria.
Background technology
Bio-denitrification technology is widely used in the production practice having obtained develop rapidly over nearly 20 years.Using maximum still is traditional digestion denitrification process, but there are problems in traditional technology, and longer as flow process, floor space is big, the initial cost height; For obtaining higher sludge concentration and nitric efficiency, need carry out mud backflow and nitrification liquid and reflux, increased power charge; Add in the alkali He in the nitrifying process and produce acidity, the denitrification process additional carbon has increased running cost; A little less than the active sludge capacity of resisting impact load; May cause secondary pollution etc.
Therefore, seek less energy-consumption, high efficiency novel denitride technology is imperative.In novel biological denitrification process, the anaerobic ammonia oxidation process advantage significantly receives much concern.Compare with traditional technology, it can save 62.5% aeration rate and 50% alkali consumption, and need not to add organic carbon source, has saved power charge greatly and has reduced running cost; In addition, because the cell yield of Anammox is well below denitrifying bacteria, the sludge yield of anaerobic ammonia oxidation process has only 15% of traditional denitrification process, alleviates the processing and the disposal of excess sludge in a large number, has reduced pollution.
The greatest problem that anaerobic ammonia oxidation process faces is: the anaerobic ammonium oxidizing bacteria poor growth, and generation time is long, and the doubling time reaches 11 days, and cell yield is low, is 0.11gVSS/gNH
4 +-N causes the enrichment time of anaerobic ammonia oxidation reactor very long, and generally in 1～1.5 year, the bioaccumulation efficiency of anaerobic ammonium oxidizing bacteria is low.For poky bacterial classification, keep its concentration in reactor most important for smooth enrichment, that is to say that can the ability that reactor is held back mud often determines successful enrichment anaerobic ammonium oxidation process.Utilize membrane bioreactor that muddy water is separated fully, make the thorough isolating characteristics of hydraulic detention time (HRT) and sludge age, membrane bioreactor and anaerobic ammonia oxidation process are combined, develop the denitrification process that the external placed type anaerobic membrane bioreactor is realized Anammox.
Summary of the invention
Long in order to solve the anaerobic ammonia oxidation reactor enrichment time that existing anaerobic ammonia oxidation process faced, the problem that the anaerobic ammonium oxidizing bacteria bioaccumulation efficiency the is low and method of utilizing membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria is provided.
The present invention utilizes the method for membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria, this method is utilized membrane bioreactor to cultivate anaerobic ammonium oxidizing bacteria as enrichment reactor and is realized according to the following steps: one, in the membrane bioreactor inlet chest, add the simulated sewage substratum, get municipal wastewater treatment plant A/O technology anoxic biochemistry pool mud, anoxic biochemistry pool temperature is 16～20 ℃ when getting mud, mud anaerobism under room temperature environment was preserved 3～5 days, then with sludge seeding in the membrane bioreactor anaerobic jar, the inoculation condition of mud is total suspended solid concentration 9920～9940mg/L, volatile suspended solid concentration is 4300～4340mg/L, and inoculum size is 2～4L; Two, enrichment culture is carried out in the inoculation back in membrane bioreactor, the enrichment culture condition is as follows: temperature is 34～36 ℃ in the control anaerobic jar, the pH value is 7.5～8.5 during water inlet, dissolved oxygen is 0.06～0.09mg/L during water inlet, the pH value is 7.0～8.0 in the anaerobic jar, dissolved oxygen in the anaerobic jar is 0～0.01mg/L, hydraulic detention time is 2 days, mud mean residence time in membrane module is 1.4～1.6min, total organic carbon is 0～0.01mg/L during water inlet, and total organic carbon is 18～20mg/L in the anaerobic jar; The enrichment culture pattern be take after the spoil disposal quiet heavy-training mode of row's supernatant liquor or do not take after the spoil disposal quiet heavy-training mode of row's supernatant liquor, be cultured to when ammonia nitrogen and nitrite nitrogen are removed simultaneously fully in the simulated sewage substratum, promptly finish and utilize membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria; Wherein the simulated sewage substratum is with (NH in the step 1
4)
2SO
4, NaNO
2, NaNO
3, KHCO
3, KH
2PO
4, MgSO
47H
2O, CaCl
22H
2O, Fe
2(SO
4)
3, EDTA, ZnSO
47H
2O, CuSO
45H
2O, MnCl
24H
2O, NiCl6H
2O, CoCl
26H
2O, H
3BO
4, NaCeO
410H
2O and NaMoO
42H
2O be dissolved in the water preparation substratum, wherein (NH
4)
2SO
4132～1980g/L, NaNO
269～2070g/L, NaNO
30～2550g/L, KHCO
31000g/L, KH
2PO
410g/L, MgSO
47H
2O 60g/L, CaCl
22H
2O 5g/L, Fe
2(SO4)
36.25g/L, EDTA 6.25g/L, ZnSO
47H
2O 430g/L, CuSO
45H
2O 250g/L, MnCl
24H
2O 990g/L, NiCl6H
2O 190g/L, CoCl
26H
2O 240g/L, H
3BO
414g/L, NaCeO
410H
2O 210g/L and NaMoO
42H
2O 220g/L boils deoxygenation.
Membrane bioreactor can make muddy water separate fully, thoroughly separates with sludge age thereby reach hydraulic detention time (HRT).The present invention combines membrane bioreactor and anaerobic ammonia oxidation process, develops the fast enriching of external placed type anaerobic membrane bioreactor with the realization anaerobic ammonia oxidizing bacteria, and external membrane bioreactor can be stablized, operation reliably.
The membrane module of membrane bioreactor is detachable, and lays in a plurality of film silks, when the degradation of existing film silk and can't be when washing pollution abatement repeatedly, then needs the film silk that in time more renews, and old film silk is carried out matting with recycle.External membrane bioreactor used in the present invention can't wash away the film silk as built-in aeration, therefore in mud total suspended solid concentration the film pollution condition easily takes place during greater than 3000mg/L, show that film is seriously polluted, the membrane module that must more renew by transmembrane pressure sensor detecting transmembrane pressure during greater than 50MPa.The cycle that the present invention changes membrane module is 30～40 days.
The present invention utilizes being masked as that membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria process finishes: ammonia nitrogen and nitrite nitrogen are existed in a large number by the cell of removing fully simultaneously or utilize fluorescence in situ hybridization technique to detect anaerobic ammonium oxidizing bacteria in the simulated sewage substratum.
The present invention utilizes the method for membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria to realize the fast enriching cultivation of anaerobic ammonium oxidizing bacteria, enrichment time is 5～7 months, obviously shorten in the existing enrichment time in 1～1.5 year of anaerobic ammonia oxidation process, and the more traditional enriching method of this method can make the bioaccumulation efficiency of anaerobic ammonium oxidizing bacteria improve 80%～95%.
Description of drawings
Fig. 1 is that embodiment four utilizes membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria to take the fluorescence in situ hybridization technique photo of the training mode of spoil disposal and quiet sinking-row's supernatant liquor;
Fig. 2 is that embodiment five utilizes membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria not take the fluorescence in situ hybridization technique photo of the training mode of spoil disposal and quiet sinking-row's supernatant liquor.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: present embodiment is utilized the method for membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria, it is characterized in that the method for utilizing membrane bioreactor to cultivate anaerobic ammonium oxidizing bacteria as enrichment reactor realizes according to the following steps: one, in the membrane bioreactor inlet chest, add the simulated sewage substratum, get municipal wastewater treatment plant A/O technology anoxic biochemistry pool mud, anoxic biochemistry pool temperature is 16～20 ℃ when getting mud, mud anaerobism under room temperature environment was preserved 3～5 days, then with sludge seeding in the membrane bioreactor anaerobic jar, the inoculation condition of mud is total suspended solid concentration 9920～9940mg/L, volatile suspended solid concentration is 4300～4340mg/L, and inoculum size is 2～4L; Two, enrichment culture is carried out in the inoculation back in membrane bioreactor, the enrichment culture condition is as follows: temperature is 34～36 ℃ in the control anaerobic jar, the pH value is 7.5～8.5 during water inlet, dissolved oxygen is 0.06～0.09mg/L during water inlet, the pH value is 7.0～8.0 in the anaerobic jar, dissolved oxygen in the anaerobic jar is 0～0.01mg/L, hydraulic detention time is 2 days, mud mean residence time in membrane module is 1.4～1.6min, total organic carbon is 0～0.01mg/L during water inlet, and total organic carbon is 18～20mg/L in the anaerobic jar; The enrichment culture pattern be take after the spoil disposal quiet heavy-training mode of row's supernatant liquor or do not take after the spoil disposal quiet heavy-training mode of row's supernatant liquor, be cultured to when ammonia nitrogen and nitrite nitrogen are removed simultaneously fully in the simulated sewage substratum, promptly finish and utilize membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria; Wherein the simulated sewage substratum is with (NH in the step 1
4)
2SO
4, NaNO
2, NaNO
3, KHCO
3, KH
2PO
4, MgSO
47H
2O, CaCl
22H
2O, Fe
2(SO4)
3, EDTA, ZnSO
47H
2O, CuSO
45H
2O, MnCl
24H
2O, NiCl6H
2O, CoCl
26H
2O, H
3BO
4, NaCeO
410H
2O and NaMoO
42H
2O be dissolved in the water preparation substratum, wherein (NH
4)
2SO
4132～1980g/L, NaNO
269～2070g/L, NaNO
30～2550g/L, KHCO
31000g/L, KH
2PO
410g/L, MgSO
47H
2O 60g/L, CaCl
22H
2O 5g/L, Fe
2(SO4)
36.25g/L, EDTA 6.25g/L, ZnSO
47H
2O 430g/L, CuSO
45H
2O 250g/L, MnCl
24H
2O 990g/L, NiCl6H
2O190g/L, CoCl
26H
2O 240g/L, H
3BO
414g/L, NaCeO
410H
2O 210g/L and NaMoO
42H
2O 220g/L boils deoxygenation.
Embodiment two: what present embodiment and embodiment one were different is that anoxic biochemistry pool temperature is 17～19 ℃ when getting mud in the step 1, and mud anaerobism under room temperature environment was preserved 4 days, and inoculum size is 3L.Other steps are identical with embodiment one with parameter.
Embodiment three: what present embodiment was different with embodiment one or two is that spoil disposal was meant in enrichment culture in the time of 1～20 day in the step 2, discharges the 50mL mud mixture every day; Quiet sinking-row's supernatant liquor is meant in enrichment culture in the time of 1～20 day, stopper film bio-reactor operation every day 30min, the quiet heavy 30min of mud in the membrane bioreactor, the liquid of then getting rid of supernatant liquor volume fraction 75% in the membrane bioreactor by siphonage, replenish subsequently with the simulated sewage substratum of getting rid of the liquid equal volume to membrane bioreactor.Other steps are identical with embodiment one or two with parameter.
Embodiment four: present embodiment utilizes the method for membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria to realize according to the following steps: one, add the simulated sewage substratum in the membrane bioreactor inlet chest, get municipal wastewater treatment plant A/O technology anoxic biochemistry pool mud, anoxic biochemistry pool temperature is 18.9 ℃ when getting mud, mud anaerobism under room temperature environment was preserved 4 days, then with sludge seeding in the membrane bioreactor anaerobic jar, the inoculation condition of mud is total suspended solid concentration 9937mg/L, volatile suspended solid concentration 4322mg/L, inoculum size is 3L; Two, enrichment culture is carried out in the inoculation back in membrane bioreactor, the enrichment culture condition is as follows: temperature is 34～36 ℃ in the control anaerobic jar, the pH value is 7.5～8.5 during water inlet, dissolved oxygen is 0.06～0.09mg/L during water inlet, and the pH value is 7.0～8.0 in the anaerobic jar, and the dissolved oxygen in the anaerobic jar is 0～0.01mg/L, hydraulic detention time is 2 days, mud mean residence time in membrane module is 1.5min, and total organic carbon is 0mg/L during water inlet, and total organic carbon is 18～20mg/L in the anaerobic jar; The enrichment culture pattern be take after the spoil disposal quiet heavy-training mode of row's supernatant liquor, be cultured to when ammonia nitrogen and nitrite nitrogen are removed simultaneously fully in the simulated sewage substratum, promptly finish and utilize membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria.
The simulated sewage medium component is shown in following table 1 and table 2 in the present embodiment step 1:
Table 1
Table 2
Present embodiment adopts fluorescence in situ hybridization technique to detect the anaerobic ammonium oxidizing bacteria bioaccumulation efficiency.
Fluorescence in situ hybridization technique detects and can select to number S-in the present embodiment
*-Amx-0368-a-A-18 (CCTTTCGGG-CATTGCGAA) and numbering S-
*The probe of-Amx-0820-a-A-22 (AAAACCCCTCTACTTAGTGCCC) is tested.
Present embodiment utilize membrane bioreactor take after the spoil disposal quiet heavy-the fluorescence in situ hybridization technique photo of the training mode enrichment culture anaerobic ammonium oxidizing bacteria of row's supernatant liquor as shown in Figure 1, present embodiment Billy do not take with membrane bioreactor spoil disposal and quiet heavy-efficient of arranging the training mode enrichment anaerobic ammonium oxidizing bacteria of supernatant liquor improved 30%.
Embodiment five: present embodiment utilizes the method for membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria to realize according to the following steps: one, add the simulated sewage substratum in the membrane bioreactor inlet chest, get municipal wastewater treatment plant A/O technology anoxic biochemistry pool mud, anoxic biochemistry pool temperature is 18.9 ℃ when getting mud, mud anaerobism under room temperature environment was preserved 4 days, then with sludge seeding in the membrane bioreactor anaerobic jar, the inoculation condition of mud is total suspended solid concentration 9937mg/L, volatile suspended solid concentration 4322mg/L, inoculum size is 3L; Two, enrichment culture is carried out in the inoculation back in membrane bioreactor, the enrichment culture condition is as follows: temperature is 34～36 ℃ in the control anaerobic jar, the pH value is 7.5～8.5 during water inlet, dissolved oxygen is 0.06～0.09mg/L during water inlet, and the pH value is 7.0～8.0 in the anaerobic jar, and the dissolved oxygen in the anaerobic jar is 0～0.01mg/L, hydraulic detention time is 2 days, mud mean residence time in membrane module is 1.5min, and total organic carbon is 0mg/L during water inlet, and total organic carbon is 18～20mg/L in the anaerobic jar; The enrichment culture pattern for do not take after the spoil disposal quiet heavy-training mode of row's supernatant liquor, be cultured to when ammonia nitrogen and nitrite nitrogen were removed fully by the while in the simulated sewage substratum, promptly finish and utilize membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria.
Present embodiment utilize membrane bioreactor do not take spoil disposal and quiet heavy-the fluorescence in situ hybridization technique photo of the training mode enrichment culture anaerobic ammonium oxidizing bacteria of row's supernatant liquor as shown in Figure 2, present embodiment has improved 80% than the efficient of the traditional cultural method enrichment anaerobic ammonium oxidizing bacteria that does not utilize membrane bioreactor.
Claims (3)
1. utilize the method for membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria, it is characterized in that the method for utilizing membrane bioreactor to cultivate anaerobic ammonium oxidizing bacteria as enrichment reactor realizes according to the following steps: one, in the membrane bioreactor inlet chest, add the simulated sewage substratum, get municipal wastewater treatment plant A/O technology anoxic biochemistry pool mud, anoxic biochemistry pool temperature is 16～20 ℃ when getting mud, mud anaerobism under room temperature environment was preserved 3～5 days, then with sludge seeding in the membrane bioreactor anaerobic jar, the inoculation condition of mud is total suspended solid concentration 9920～9940mg/L, volatile suspended solid concentration is 4300～4340mg/L, and inoculum size is 2～4L; Two, enrichment culture is carried out in the inoculation back in membrane bioreactor, the enrichment culture condition is as follows: temperature is 34～36 ℃ in the control anaerobic jar, the pH value is 7.5～8.5 during water inlet, dissolved oxygen is 0.06～0.09mg/L during water inlet, the pH value is 7.0～8.0 in the anaerobic jar, dissolved oxygen in the anaerobic jar is 0～0.01mg/L, hydraulic detention time is 2 days, mud mean residence time in membrane module is 1.4～1.6min, total organic carbon is 0～0.01mg/L during water inlet, and total organic carbon is 18～20mg/L in the anaerobic jar; The enrichment culture pattern be take after the spoil disposal quiet heavy-training mode of row's supernatant liquor or do not take after the spoil disposal quiet heavy-training mode of row's supernatant liquor, be cultured to when ammonia nitrogen and nitrite nitrogen are removed simultaneously fully in the simulated sewage substratum, promptly finish and utilize membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria; Wherein the simulated sewage substratum is with (NH in the step 1
4)
2SO
4, NaNO
2, NaNO
3, KHCO
3, KH
2PO
4, MgSO
47H
2O, CaCl
22H
2O, Fe
2(SO4)
3, EDTA, ZnSO
47H
2O, CuSO
45H
2O, MnCl
24H
2O, NiCl6H
2O, CoCl
26H
2O, H
3BO
4, NaCeO
410H
2O and NaMoO
42H
2O be dissolved in the water preparation substratum, wherein (NH
4)
2SO
4132～1980g/L, NaNO
269～2070g/L, NaNO
30～2550g/L, KHCO
31000g/L, KH
2PO
410g/L, MgSO
47H
2O 60g/L, CaCl
22H
2O 5g/L, Fe
2(SO4)
36.25g/L, EDTA 6.25g/L, ZnSO
47H
2O430g/L, CuSO
45H
2O 250g/L, MnCl
24H
2O 990g/L, NiCl6H
2O 190g/L, CoCl
26H
2O 240g/L, H
3BO
414g/L, NaCeO
410H
2O 210g/L and NaMoO
42H
2O 220g/L boils deoxygenation.
2. the method for utilizing membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria according to claim 1, anoxic biochemistry pool temperature is 17～19 ℃ when it is characterized in that getting mud in the step 1, and mud anaerobism under room temperature environment was preserved 4 days, and inoculum size is 3L.
3. the method for utilizing membrane bioreactor enrichment culture anaerobic ammonium oxidizing bacteria according to claim 1 and 2 is characterized in that spoil disposal was meant in enrichment culture in the time of 1～20 day in the step 2, discharges the 50mL mud mixture every day; Quiet sinking-row's supernatant liquor is meant in enrichment culture in the time of 1～20 day, stopper film bio-reactor operation every day 30min, the quiet heavy 30min of mud in the membrane bioreactor, the liquid of then getting rid of supernatant liquor volume fraction 75% in the membrane bioreactor by siphonage, replenish subsequently with the simulated sewage substratum of getting rid of the liquid equal volume to membrane bioreactor.
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| CN102627379A (en) * | 2012-03-27 | 2012-08-08 | 杭州师范大学 | Method for storing and exciting anaerobic ammonium oxidation sludge |
| CN103601292A (en) * | 2013-12-06 | 2014-02-26 | 哈尔滨工业大学 | Culture method of AOB-ANAMMOX granular sludge |
| CN103710287A (en) * | 2013-12-25 | 2014-04-09 | 天津凯英科技发展有限公司 | Culturing method for ammonia oxidizing bacteria |
| CN105236568A (en) * | 2015-08-07 | 2016-01-13 | 北京大学 | Method for running anammox by utilizing microbial immobilization membrane bioreactor |
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| CN111394429A (en) * | 2020-04-13 | 2020-07-10 | 唐山师范学院 | Novel fluorescence in-situ hybridization detection method for microbial culture |
| CN111547866A (en) * | 2020-06-11 | 2020-08-18 | 北京工业大学 | Device and method for culturing and enriching anaerobic ammonium oxidation sludge |
| CN111547866B (en) * | 2020-06-11 | 2021-12-07 | 北京工业大学 | Device and method for culturing and enriching anaerobic ammonium oxidation sludge |
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| CN115536145A (en) * | 2021-06-29 | 2022-12-30 | 中国石油化工股份有限公司 | Enrichment culture method and device for anaerobic ammonium oxidation bacteria |
| CN113603311A (en) * | 2021-09-10 | 2021-11-05 | 上海环境保护有限公司 | Agent and process for removing total nitrogen in brewing wastewater |
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