CN102524115A - Method and equipment for cultivating oryzias latipes artificially - Google Patents
Method and equipment for cultivating oryzias latipes artificially Download PDFInfo
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- CN102524115A CN102524115A CN2010106140642A CN201010614064A CN102524115A CN 102524115 A CN102524115 A CN 102524115A CN 2010106140642 A CN2010106140642 A CN 2010106140642A CN 201010614064 A CN201010614064 A CN 201010614064A CN 102524115 A CN102524115 A CN 102524115A
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention relates to a method and equipment for cultivating oryzias latipes artificially. The method comprises the following steps of: feeding the oryzias latipes under the controllable indoor environment, filling tap water which is aerated for 2 to 3 days or river water which is subjected to sand filtration into an aquarium, cultivating oryzias latipes at the temperature of below 30 DEG C, and putting artificial fish nests, wherein the feeding density of parent fish is below 2 fish/L; and putting fish fries into a floating net cage for feeding. The method and the equipment are suitable for the large-scale cultivation of the oryzias latipes, and have the characteristics of high yield, high controllability and the like.
Description
Technical field
The invention belongs to animal farming industry, be specifically related to a kind of artificial cultivating method and cultivation equipment of medaka.
Background technology
Medaka belongs to Chordata, Vertebrate, net-rope, bony fish subclass, Medaka shape order, jaw Medaka suborder, Medaka section, Medaka genus.The distribution of medaka is more extensive, can adapt to the weather conditions of Chinese most of areas, and at the degree of saltiness aqua region in area, river mouth distribution is arranged also.
The biological study background of medaka very fully.Medaka is approved by the international organization of majority as a kind of laboratory animal.Because medaka is comparatively responsive to the variation of water quality, is classified as toxicity test the eighties in 20th century by International Standards Organization and one of is planted experimentally.At present, medaka is applied in the multiple water quality monitoring widely.
Yet owing to the pressure of environment damage and species invasion, medaka is fewer and feweri in the distribution of occurring in nature at present.Simultaneously, the source of medaka is main to fish for still, and it is less that artificial culture work is carried out, and existing artificial culture mode also exists a series of problems such as output is lower of cultivating simultaneously, and therefore present scale is cultivated and had certain difficulty.
Summary of the invention
The objective of the invention is to overcome the defective of prior art, a kind of artificial cultivating method and cultivation equipment of medaka is provided.
The present invention adopts following technical scheme to solve the problems of the technologies described above:
The artificial cultivating method of a kind of medaka (O.latipes); Be under indoor controlled environment, to raise medaka; In aquarium, inject 2～3 days running water of aeration or through the river of husky filter, the temperature in the breeding process is below 30 ℃, be the basis in the volume of water in the aquarium; Kind of fish stocking density is 2 tails/below the L, and throws in artificial fish nest; Fry places the floating type net cage to raise.
Preferable, the temperature of said breeding water body is below 30 ℃, further is preferably 22 ℃～29 ℃.Generally, the temperature of breeding water body is 22 ℃～26 ℃, and during hatch fish roe, the temperature of breeding water body is adjusted into 27～29 ℃, preferred 28 ℃.
Preferable, said inoculum density (promptly planting the fish stocking density) further is preferably 1～2 tail/L, 1 tail/L most preferably, and female-male proportion is preferably 2～3: 2.
Breeding water selects 2～3 days running water of aeration for use or through the husky river of filtering, water level generally is lower than container upper limb 5～8cm.Water body oxygen content DO＞5mg/L.
Volume in water in the floating type net cage is the basis, and fry density is preferably 5～6 tails/L.The floating type net cage places aquarium.
Preferable, in the breeding process of medaka, plant fish and need throw something and feed at regular time and quantity.Preferred bait is Daphnia magna, thin slice feed etc., and most preferred bait is Daphnia magna; Said bait injected volume is every day 3 times, is 4%～6% of medaka body weight at every turn, and most preferred injected volume is 5% of a medaka body weight.The fry that hatches is used pulvis feed or halogen worm just incubates nauplius and feeds every day 5 times.
Preferable, in the breeding process of medaka, need to use the biochemistry filtered device of gas stripping type to promote water circulation, carry out water treatment.
Preferable, in the breeding process of medaka, need regularly change water according to the situation of water quality, adopt siphonage to extract former water out part (as 1/3), add through 2～3 days running water of aeration or through the river of husky filter, the temperature difference of water temperature is no more than ± and 1 ℃.
Preferable, in the medaka breeding process, need to throw in artificial fish nest or pasture and water, be preferably the artificial fish nest that fortune windmillpalm sheath-fibre or nylon wire are processed, the fish nest is a planar structure, the artificial fish nest of most preferred artificial fish nest for being processed by 30 order nylon wires.
Preferably, the preparation method of described artificial fish nest is: using mesh is the green nylon wires of 30 purposes, cuts out model and is long 15cm, wide 10cm.Fish nest lower edge is made weight, and fish nest upper limb is made the nylon wire of two long 20cm.
Preferable, place 10 artificial fish nests in the single aquarium, all artificial fish nests were gathered in per 8 days, and place the floating type net cage to hatch.
The artificial cultivating method of optimum medaka comprises the steps:
1) medaka kind fish screening: screening is healthy, great-hearted medaka, and removes harmful organisms;
2) raising of medaka kind fish: the river that in aquarium, injects 2～3 days running water of aeration or filter through sand; Temperature in the breeding process is 22～26 ℃; The kind fish that screening is good is according to female: hero is that 3: 2 ratio is put into aquarium with medaka and raised, and density is 1 tail/L;
3) hatching of medaka ovum: the artificial fish nest that has the medaka ovum gathered once in per 8 days, the fish-egg of gathering places dissolved oxygen to hatch greater than the water body of 5mg/L, and water temperature is controlled at 28 ℃;
4) raising of medaka seedling: the fry that hatches is used pulvis feed or halogen worm just incubates nauplius and feeds every day 5 times.
Preferable, the first of halogen worm incubates nauplius through the green alga reinforcement.
The halogen worm that green alga was strengthened can be adopted and used the bait that contains green alga to raise the acquisition of halogen worm.
In the step 1, described health, great-hearted medaka is meant: body surface does not have parasite, and is disease-free, the maturation kind fish below 1 age.Said harmful organisms comprises: nymph of the dragonfly 、 Acenthogobius flavimanus etc.
Preferably, in the said step 2, the condition of water quality that the water quality of assurance breeding water body and kind are originated during raising differs can not be too big, guarantees the water temperature domain of walker within 1～2 ℃, and pH value fluctuation range is within 0.1～0.3 scope.
The invention also discloses a kind of cultivation equipment of medaka, this cultivation equipment is aquarium and supporting artificial fish nest and the floating type net cage of growing seedlings.
The preferred 36L of said aquarium right angle glass aquarium is of a size of 60cm * 30cm * 20cm.
Described artificial fish nest is a planar structure.Size can be 15cm * 10cm, and mesh size is 30 orders.
The said floating type net cage floor space of growing seedlings is 90cm
2, height is 30cm, mesh diameter is 0.25～0.28mm, and behind the aquarium water-filling, the floating type net cage floatability of growing seedlings, when net cage was floating, the upper surface of net cage was above water, and the upper surface of said net cage and the vertical range of horizontal plane are 0.1～0.2mm.
Preferable, the cultivation equipment of said medaka also is provided with water circulation system and Temperature-controlled appliance.The preferred gas stripping type filter of water circulation system.
Cultural method of the present invention has following characteristics:
The present invention utilizes totally-enclosed indoor controlled cultivating system, in aquarium, raises medaka, and according to the rule that medaka is laid eggs, the brooding time of control fish-egg is to reach the purpose of high density hatching.Guarantee the quality of the medaka of output simultaneously, made things convenient for standardized work.Be primarily characterized in that: under indoor controlled environmental condition, raise medaka, keep water temperature to be stabilized in 22～26 ℃; Oxygen content DO＞5mg/L during the breed, carries out throwing something and feeding for three times to kind of fish and through the regulation and control of temperature being carried out the concentrated hatching of fish-egg every day.
The present invention also utilizes the biochemistry filtered device of gas stripping type to promote water circulation to make the oxygen of aquarium sufficient, makes it possible to carry out high density, and the breed of high water body availability improves water quality simultaneously.
Compared with prior art; Adopting method of the present invention to carry out medaka cultures; Have breeding water body little, pollute little, culture controllability strong, culture efficient high, fish for conveniently, characteristics such as output and quality height, simple and convenient management, a large amount of standardization laboratory animal can be provided for the Experiment of Zoology of China.
Description of drawings
Fig. 1 medaka cultivator of the present invention sketch map.
Fig. 2 floating type cage structure sketch map of growing seedlings.
Fig. 3 fish nest structural representation.
Embodiment
Further describe technical scheme of the present invention through specific embodiment below.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.
Embodiment 1
Medaka cultivator as shown in fig. 1, this device comprise closed aquarium and the supporting artificial fish nest and the net cage of growing seedlings.
Closed aquarium is a 36L right angle glass aquarium, is of a size of 60cm * 30cm * 20cm.
Artificial fish nest is as shown in Figure 3, is planar structure, is of a size of 15cm * 10cm, and mesh size is 30 orders, and top is provided with to fix uses nylon rope, and the bottom is provided with weight.
The net cage of growing seedlings is as shown in Figure 2, and the net cage floor space of growing seedlings is 90cm
2, height is 30cm, and mesh diameter is 0.25～0.28mm, and the upper surface of net cage is above water, and the vertical range of upper surface and horizontal plane is 0.1～0.2mm, and its top is provided with buoyant module.
The artificial cultivating method of medaka:
Screen health, great-hearted medaka kind fish and remove harmful organisms in spring;
The river that in aquarium, injects 2～3 days running water of aeration or filter through sand is raised the medaka after the screening in aquarium, and stocking density is 1 tail/L, and female-male proportion is 3: 2; And placement artificial fish nest; In breeding process, maintain the temperature between 22～26 ℃, and throw something and feed at regular time and quantity every day, bait is Daphnia magna or thin slice feed, and the bait injected volume is every day 3 times, is 4%～6% of medaka body weight at every turn; With 8 days was the cycle artificial fish nest to be gathered.
The artificial fish nest that takes out is positioned over concentrates hatching in the floating type net cage, temperature is controlled at 28 ± 1 ℃.
The medaka prelarva that hatches uses pulvis feed or artemia nauplii to throw something and feed every day 5 times.
In the breeding process of medaka, regularly change water according to the situation of water quality, adopt siphonage to extract former water out 1/3, add through 2～3 days running water of aeration or through the river of husky filter, the temperature difference of water temperature is no more than ± and 1 ℃.
The dissolved oxygen that in whole breeding process, uses the biochemistry filtered device assurance of gas stripping type water body guarantees that simultaneously the water body quality is good more than 5mg/L.
Adopt above-mentioned raising method, the medaka survival rate reaches 70%, medaka year output reach 2400 tails/L.
Claims (13)
1. the artificial cultivating method of a medaka; Be under indoor controlled environment, to raise medaka; In aquarium, inject 2～3 days running water of aeration or through the river of husky filter, the temperature in the breeding process is below 30 ℃, plant the fish stocking density and be 2 tails/below the L; And the input artificial fish nest, fry places the floating type net cage to raise.
2. the artificial cultivating method of medaka according to claim 1 is characterized in that the temperature of said breeding water body is 22 ℃～29 ℃, the oxygen content DO of breeding water body＞5mg/L.
3. the artificial cultivating method of medaka according to claim 1 is characterized in that, is the basis in the volume of water in the aquarium, and said kind of fish stocking density is 1～2 tail/L, and planting the fish female-male proportion is 2～3: 2; Volume in water in the floating type net cage is the basis, and the fry stocking density is 5～6 tails/L.
4. the artificial cultivating method of medaka according to claim 1 is characterized in that, in the breeding process of medaka, plants fish and throws something and feeds at regular time and quantity, and bait is Daphnia magna or thin slice feed, and the bait injected volume is every day 3 times, is 4%～6% of medaka body weight at every turn; The fry that hatches is used pulvis feed or halogen worm just incubates nauplius and feeds every day 5 times.
5. the artificial cultivating method of medaka according to claim 1 is characterized in that, in the breeding process of medaka, adopts the gas stripping type filter to promote water circulation, carries out water treatment.
6. the artificial cultivating method of medaka according to claim 1; It is characterized in that; In the breeding process of medaka, need regularly change water, adopt siphonage that partial raw water is extracted out; Add the river through 2～3 days running water of aeration or the husky filter of process, Yuan Shui is no more than 1 ℃ with the water temperature temperature difference of newly changing water.
7. the artificial cultivating method of medaka according to claim 1 is characterized in that said artificial fish nest is the artificial fish nest that fortune windmillpalm sheath-fibre or nylon wire are processed.
8. the artificial cultivating method of medaka according to claim 1 is characterized in that, all artificial fish nests is gathered in per 8 days, and places the floating type net cage to hatch.
9. like the artificial cultivating method of the arbitrary said medaka of claim 1～8, it is characterized in that the artificial cultivating method of said medaka comprises the steps:
1) medaka kind fish screening: screening is healthy, great-hearted medaka, and removes harmful organisms;
2) raising of medaka kind fish: the river that in aquarium, injects 2～3 days running water of aeration or filter through sand; Temperature in the breeding process is 22～26 ℃; The kind fish that screening is good is according to female: hero is that 3: 2 ratio is put into aquarium with medaka and raised, and density is 1 tail/L;
3) hatching of medaka ovum: the artificial fish nest that has the medaka ovum gathered once in per 8 days, the fish-egg of gathering places dissolved oxygen to hatch greater than the water body of 5mg/L, and water temperature is controlled at 27～29 ℃;
4) raising of medaka seedling: the fry that hatches is used pulvis feed or halogen worm just incubates nauplius and feeds every day 5 times.
10. the cultivation equipment of a medaka comprises aquarium and the supporting artificial fish nest and the net cage of growing seedlings.
11. the cultivation equipment like the said medaka of claim 10 is characterized in that, said aquarium is a 36L right angle glass aquarium; Described artificial fish nest is a planar structure, is of a size of 15cm * 10cm, and mesh size is 30 orders; The said net cage floor space of growing seedlings is 90cm
2, height is 30cm, and mesh diameter is 0.25～0.28mm, and when the said net cage of growing seedlings was floating, the upper surface of net cage was above water, and the vertical range of the upper surface of said net cage and horizontal plane is 0.1～0.2mm.
12. the cultivation equipment like claim 10 or 11 said medakas is characterized in that the cultivation equipment of said medaka also is provided with water circulation system and Temperature-controlled appliance.
13. the cultivation equipment like the said medaka of claim 12 is characterized in that, said water circulation system is the gas stripping type filter.
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| CN2010106140642A CN102524115A (en) | 2010-12-30 | 2010-12-30 | Method and equipment for cultivating oryzias latipes artificially |
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| CN2010106140642A CN102524115A (en) | 2010-12-30 | 2010-12-30 | Method and equipment for cultivating oryzias latipes artificially |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103518654A (en) * | 2013-09-18 | 2014-01-22 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Killifish cultivation method |
| CN108633794A (en) * | 2018-03-21 | 2018-10-12 | 浙江师范大学 | A kind of method for arranging of black pearl Medaka fish breeding spawning tank |
| WO2020073280A1 (en) * | 2018-10-10 | 2020-04-16 | 苏州鱼之源生物科技有限公司 | Block-type ecology-imitating pond breeding and fry-rearing device and method |
| CN111248115A (en) * | 2020-01-10 | 2020-06-09 | 山东省海洋生物研究院 | Indoor culture method for obtaining high-quality fertilized eggs of seawater medaka |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103518654A (en) * | 2013-09-18 | 2014-01-22 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Killifish cultivation method |
| CN108633794A (en) * | 2018-03-21 | 2018-10-12 | 浙江师范大学 | A kind of method for arranging of black pearl Medaka fish breeding spawning tank |
| CN108633794B (en) * | 2018-03-21 | 2020-10-23 | 浙江师范大学 | Arrangement method of egg laying vat for breeding black pearl killifish |
| WO2020073280A1 (en) * | 2018-10-10 | 2020-04-16 | 苏州鱼之源生物科技有限公司 | Block-type ecology-imitating pond breeding and fry-rearing device and method |
| CN111248115A (en) * | 2020-01-10 | 2020-06-09 | 山东省海洋生物研究院 | Indoor culture method for obtaining high-quality fertilized eggs of seawater medaka |
| CN111248115B (en) * | 2020-01-10 | 2021-10-26 | 山东省海洋生物研究院 | Indoor culture method for obtaining high-quality fertilized eggs of seawater medaka |
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Application publication date: 20120704 |