CN102485237B - Sweet wormwood herb brewed liquid, and brewing technology thereof - Google Patents

Abstract

The invention relates to sweet wormwood herb brewed liquid, which is a pure brewed liquid brewed with a modified ancient traditional fermentation technology and with sweet wormwood herb and glutinous rice as raw materials. A main effective component of sweet wormwood herb, artemisinin, exists in plant cell glands, and is not soluble in water. Currently, both organic solvent extraction and chemical synthesis are disadvantaged in complicated technology, high cost and a problem of toxicity. According to the invention, a multi-strain starter which has both functions of saccharification and fermentation, a rice feeding method, and a composite fermentation technology are adopted. The technology is characterized in that: the temperature during the whole brewing process is controlled below 35 DEG C; a fermentation period is long (above 90 days); and prepared sweet wormwood herb medicinal brewed liquid contains various nutrients and complete effective components in sweet wormwood herb. As a result of cytotoxic tests upon breast cancer cells and lung cancer cells, the cells can be completely killed within 20h. The brewed liquid is taken by more than 20 people. It is found that the brewed liquid has certain ideal effects against some stubborn diseases, and no toxic or side effect is found.

Description

Herba Artemisiae Annuae is made liquid and brewage thereof

Technical field

The present invention relates to the production technology that Chinese herbal medicine is made, the manufacture method that particularly relates to a kind of anti-malignant tumor, prevents and treats Herba Artemisiae Annuae medicine wine and brewage and its " song " of multiple difficult and complicated illness, belongs to field of medicaments.

Background technology

Chinese herbal artemisia, comes from motherland's traditional medicine treasure-house, and nature and flavor bitter cold is nontoxic, returns liver, gallbladder, kidney channel, and there is heat clearing away, drive away summer heat, remove the effects such as steaming, preventing the attack (or recurrence) of malaria, be traditional heat clearing away and summer-heat relieving medicine.Herba Artemisiae Annuae is apart from outside malaria function, still have the immunity of adjusting, anti-schistosomiasis, antiviral, antibacterial, antiendotoxin and eliminate the phlegm, antitussive, the effect such as relieving asthma; Increasing research is in recent years put in the antitumor research of artemisine compounds.

The arteannuin extracting from Herba Artemisiae Annuae, is the sesquiterpene lactones medicine that has peroxy-radical, by World Health Organization (WHO), is regarded as the alternative the most effective antimalarial of quinine of 21 century.Modern pharmacological research confirmation, so arteannuin only can be treated malaria, its mechanism of action is after by it, the high ferrous components in plasmodium is combined, and chemical reaction occurs, generation can destroy " free radical " of cell membrane, thereby makes unicellular plasmodium dead.Just based on this principle, scientists thinks, Herba Artemisiae Annuae have the potentiality for the treatment of cancer.

The research report that U.S. life sciences magazine (LS) publishes says, arteannuin can almost kill all human breast cancer cells of contact with it in 16 hours, claimed " it likely becomes nontoxic anticarcinogen ".Separately there are some researches show, after leukaemia contacts with arteannuin, in 8 hours, just eliminated.Research worker has also been done some tests: for example a Canis familiaris L. that suffers from serious osteocarcinoma can not walk, within 5 days after accepting arteannuin treatment, just recovers walking.About the problem of " arteannuin is expected to become anticarcinogen ", have a lot of reports both at home and abroad.

Arteannuin derives from the leaf of Herba Artemisiae Annuae, and it is mainly present in the body of gland of plant cell, belongs to secondary metabolite in born of the same parents.Extract medium and first from feed particles outside, diffuse in granule interior and cell and dissolve arteannuin, due to inside and outside arteannuin concentration difference, arteannuin diffuses into main body solution again.Arteannuin is water insoluble, and in oil, dissolubility is also little; After when extracting, temperature surpasses 60 degree Celsius, peroxide bridge structure is destroyed very soon, loses drug effect completely.Current commercial production is extracted the method for arteannuin, is by artemisia leaf powder, with 120# gasoline lixiviate 2-3 time, separates out crude product, then use 50%, 95% ethyl alcohol recrystallization through concentrating under reduced pressure, then through separated, purification, obtain white arteannuin.Content of artemisinin in sweet wormwood only has 0.1-1%, lower than 0.5%, does not just have industrial abstract to be worth.By conventional organic solvents, stir and extract arteannuin, as high and stable in ether extract drug effect, but dosage is bigger than normal, and show toxicity after dosage increase, and need be further purified, better concentrate active substance, improve curative effect.Extraction link is many, wastes time and energy, and cost is high, yields poorly, and the problems such as toxicity, are difficult to satisfy the demand.

The mensuration of < < arteannuin structure and complete synthesis through in > > mono-literary composition: " Herba Artemisiae Annuae is a strain JIUMINGCAO ", arteannuin structure and complete synthesis realization, for the exploitation of arteannuin drug derivative is laid a good foundation, but itself can not put into production because complex process, cost are too high." (source: science Times date issued: 2008-12-02)

American scientist has been found a kind of new enzyme synthetic relevant with artelinic acid in Herba Artemisiae Annuae.To manufacture after the gene insertion saccharomyces cerevisiae of this kind of enzyme, yeast has produced the precursor substance of arteannuin---artelinic acid.Utilize microorganism synthetic chemical to increase output, to reduce costs, the expense that be expected to significantly to increase arteannuin output, reduces treatment malaria.But be put to practicality and at least also need several years.(source: Xinhua News Agency's typing time: 08.08。02。)

Magical drug effect due to Herba Artemisiae Annuae, has attracted world's sight.Current target is to develop that cost is low, price is low, can be the non-toxic oral medicine that outpatient accepts, to reach the object of " whole world is shared cheap ".The complete works of > > of < < China medicated wine (Shanghai science tech publishing house publishes in May, 1991) " preface " points out: " just having Rhizoma Coptidis wine to control pyobloody dysentery as far back as Shanxi in for < Zhouhou Beiji Fang >; the record of Herba Artemisiae Annuae wine treating malaria " " experiment also shows; some medicine is made after medicated wine, has that other dosage form does not have or incomparable effect far away ".In the 113rd page of this book, have that " Herba Artemisiae Annuae wine cures mainly asthenia chronic malaria.Preparation method: Herba Artemisiae Annuae is smash juice, decocts, wine brewing as usual.Source of data: bright. < < Compendium of Materia Medica > >.”

Summary of the invention

The object of the invention is:

1, provide a kind of fermentation technique that Herba Artemisiae Annuae condition, the low extraction ratio of cost are high, can produce in batches that meets.

2, so that a kind of whole active ingredient in Herba Artemisiae Annuae, nontoxic pure wine liquid of Herba Artemisiae Annuae herb of containing to be provided.

3, take provide a kind of price low, can as outpatient's malaria that accept, that be applicable to child, anti-malignant tumor and regulate immunologic function, antiendotoxin, antibacterial, antiviral, parasiticide and eliminate the phlegm, the pure Chinese herbal medicine of Herba Artemisiae Annuae oral, external of antitussive, the effect such as relieving asthma.

For achieving the above object, technical solution of the present invention is:

1, adopt the thinking foundation of brew Herba Artemisiae Annuae, the active ingredient that goes out Herba Artemisiae Annuae with abundant brew.

Ancient traditional yellow rice wine is called as nutritive beverage, and it is to take Oryza glutinosa as raw material, adopts " song " to carry out compound fermentation, and it is extremely abundant that energy brew goes out nutrition, as contained 21 seed amino acids, wherein 8 kinds of essential amino acid A wide selection of colours and designs; Containing higher functional oligose; Detected inorganic salt has 18 kinds more than, comprises the macroelements such as calcium, magnesium, potassium, phosphorus and ferrum, copper, zinc, selenium and other trace elements; Also containing physiologically active ingredients such as polyphenol substance, melanoidin, glutathion.

2, brew Herba Artemisiae Annuae has following main points:

(1) medicinal ingredient such as arteannuin mostly is intracellular product, and during extraction, effective ingredient discharges in born of the same parents, diffuses into that to extract medium slow, therefore will select yeast strong " song ", and yeast phase will be grown;

(2), while extracting arteannuin, after temperature surpasses 60 degree Celsius, peroxide bridge structure is destroyed very soon, loses drug effect completely.Therefore, Herba Artemisiae Annuae raw material can not be tortured through high temperature or in boiling water, and temperature will be strictly controlled in whole technological process well;

(3) Herba Artemisiae Annuae is made liquid without high temperature sterilization.Therefore upper the wanting of " song " and operation can strictly be suppressed varied bacteria growing.

Traditional zymotic technique is being improved in the brew that is applied to Chinese herbal medicine, the selection-breeding that " song " planted, raised and train, and the operational approach of brew, carried out continuous exploration, research and summary, raising.

The specific embodiment

The fermentation technique of Herba Artemisiae Annuae

Raw material: Oryza glutinosa, Herba Artemisiae Annuae (preferably the leaf of fresh grass or cut off then the leaf drying), bent, water.

Ratio (jin): 100 jin, rice; Approximately 50 jin of Herba Artemisiae Annuae cured leafs; Bent approximately 1 jin; Approximately 150 jin, water.

The principle of brew and operational approach:

Should note some:

The sanitation and hygiene that 1, will keep fermenting cellar, equipment and staff;

2, brew is many in cold season, and the south of the River is many in the Beginning of Winter, and stop the beginning of spring.In Xi'an, can start in October.Harmful microorganism is difficult for growth and breeding at low temperatures, and is conducive to long-time slowly fermenting.

3, strict controlled fermentation temperature, prevents from becoming sour.Front ferment temperature drift, ferment middle yeast falls into a decline, and easily causes living contaminants breeding and becomes sour.The temperature of after fermentation phase is preferably controlled at below 15 degree.

4, produce and carry out in cylinder, altar, adopt the operating procedure for micro-oxygen.The airing function that the pore of cylinder, altar has can discharge in time carbon dioxide, absorb fresh air, impels yeast constantly to breed yeast phase long.

Operational approach

1, clean artemisia leaf is pulverized, by " song " grinds, standby;

2, Oryza glutinosa soaks, cooks rear cold water pouring but, makes to be cooled to 28～30 ℃, enters vat mixed song, takes unstrained spirits, adds a cover insulation;

3, after 24-30 hour, go out to make liquid, start cylinder cap, cylinder temperature is lowered, and can discharge carbon dioxide, oxygen gas-supplying, promotes fermentation;

4, after 24 hours, make piece again and float, pour in proportion cold water into, stripping and slicing, upset;

5,, again after one day, feeding, pours 30 jin of Herba Artemisiae Annuaes into for the first time, adds song and mixes thoroughly, carries out prior fermentation.Use feeding method, in the situation that raw material progressively increases continuous fermentation, yeast constantly obtains new nutrition, and has played repeatedly amplification culture effect, can generate more yeast cells, makes yeast be difficult for senilism.

6, excessively after 12-18 hour, drive rake, mix cylinder.By stirring up and down, not only regulate product temperature, and increased to yeast oxygen-supplying amount, get rid of carbon dioxide, the absolute advantages of performance yeast in unstrained spirits;

7, next day, carry out feeding for the second time, every cylinder is poured 20 jin of Herba Artemisiae Annuaes into, adds song, mixes cylinder;

8, again after one day, pour into the little field of razor clam (30 jin of every field of razor clams), liquid level, apart from approximately 10 centimeters of Cheng Kou, ties Cheng Kou, is beneficial to carry out slowly fermenting under low temperature, hypoxia; Slowly fermenting phase length is relevant with weather, generally more than 90 days.

9, squeeze and filter

10, pour into the little field of razor clam (with Folium Nelumbinis, Folium Bambusae, mudding mouth) warehouse-in, display more than one month.

11, filter → bottling;

12, paste label → packing;

13, be up to the standards → dispatch from the factory

The manufacture method of " song "

The manufacture method of " song " has two kinds of Traditional Method and purebred methods, and we adopt is that many strains of making of Traditional Method are bent, facts have proved: many strains song that the song of single culture is made not as good as Traditional Method is good.In master worker among the people in an ancient town of millennium that retains ancient traditional brewage, select " song " that pass on and plant, then through long-term artificially breeding, raise and train, thereby obtained, meet required " song " kind of brew Herba Artemisiae Annuae condition.

Raw material: new early rice flour; Fresh Herba polygoni hydropiperis; Water; Bent female (select goodly, fragrance is good, and quality is loose, free from insect pests, and in large production, fermentation is normal, coordinates the speed between saccharifying and fermentation, and easy control of temperature, suppresses varied bacteria growing, and raw acid amount is few, yeast is strong).

Batching: new early rice flour: fresh Herba polygoni hydropiperis: water=20: (0.4-0.6): (10.5-11)

Method for making

1, extract fresh Herba polygoni hydropiperis and dry, get leaf, be ground into powder, standby;

2, new early paddy removes shell, pulverizing, sieves, and adds in proportion Herba polygoni hydropiperis powder, water, after mixing thoroughly;

3, flatten, stripping and slicing, inoculates after rolling angle;

4, dress curtain.While putting, between curved billet, keep 0.5 centimetre of space, the temperature 32-35 ℃ of culturing room, relative humidity should rest in 95-100%, as does not reach, can be to locular wall and ground watering humidification; Through 4-6 hour, spore starts to sprout;

5, after 10-16 hour, the highest product temperature should be controlled at 35 ℃, and as too high, turnable once, omits door window, and puts tide, and now relative humidity can rest in 85-90%;

6,, again through 24-28 hour, after covering with mycelia, surface looks just like a slice floss hair;

7, go out nest, shine medicine.Shine medicine temperature at 40-45 ℃, approximately shine 5-6 days altogether, now water content is 14-15%.

8, finished product (preserving in the altar that enters to make pottery).

Lime light:

1, administered the temperature of culturing room, the highest product temperature should be controlled at 35 ℃.

2, in whole operating process, operator enter culturing room all will dress clothing, cap and the shoes of sterilizing, and will sterilize by 75% alcohol hand, and note Personal hygiene during operation.

Herba Artemisiae Annuae is made the detection of liquid

Sense organ: at previous month of fermentation, be the liquid of adularescent suspended matter, have vaporific white thing in sediment; Be after two months golden transparent liquid, the vaporific white thing in sediment dies down.

Delicate fragrance, bitter in the mouth, have refrigerant sense.

Herba Artemisiae Annuae is made liquid and is detected (wine liquid during brew one and a half months)

Artemislnin content: 294.9ug/g in raw material Herba Artemisiae Annuae cured leaf (this batch of raw material Herba Artemisiae Annuae cured leaf is the acquisition of honest Pharma Inc. from Ankang, and stock is 2 years, and the content of arteannuin is obviously on the low side).

Artemislnin content in the supernatant of fermentation liquid: 1.432ug/ml

Artemislnin content: 180.7ug/g in fermentation liquid precipitate (slag)

Some is inquired into:

1, the liquid that is adularescent suspended matter cannot be done quantitative analysis, can only directly get supernatant and after micro-pore-film filtration, detect gained (the 233rd page of < < Herba Artemisiae Annuae and artemisinin-based drug > >).The brew phase, the thing that is creamy white is suspended in to be made in liquid and precipitate in short-term, and this milky thing may be exactly arteannuin.

2, Herba Artemisiae Annuae is made the sense organ variation of liquid: because of in after fermentation process, along with the prolongation of time, milky thing fades to lose and forms golden transparent liquid; Owing to being subject to the impact of microorganism and other physical chemical factors, can also generate the nitrogen substance of various higher alcohols, organic acid, esters, aldehydes, ketone and microbial cell self etc., increase lipotropy (oil-soluble) and the stability of medicine, and they itself also there is certain medical value.For example: during the fermentation because yeast vital movement produces succinic acid, whether can form the artemisinin derivatives such as artesunate class.

3, make in liquid and also contain active ingredient in arteannuin Herba Artemisiae Annuae in addition, due to the complex biochemical reaction of enzyme, and generate Herba Artemisiae Annuae derivant.

4, in Oryza glutinosa in raw material and artemisia leaf end ratio, in precipitate, Herba Artemisiae Annuae accounts for 1/3rd, and in every gram of Herba Artemisiae Annuae precipitate, artemislnin content answers * 3, that is: 180.7 * 3=542.1ug/g

Artemislnin content in fermentation liquid precipitate (sediment): artemislnin content in 542.1ug/g+ fermented supernatant fluid: the 1.432ug/ml+ overwhelming majority be artemislnin content in the liquid that has vaporific suspended matter.This explanation: with in the wine liquid of brew one and a half months of the present invention, apparently higher than the artemislnin content (294.9ug/g) of organic solvent extraction.

5, the Herba Artemisiae Annuae cured leaf of this batch of raw material is the acquisition of honest Pharma Inc. from Ankang, and stock is 2 years, and the content of arteannuin is obviously on the low side, has not been suitable for current suitability for industrialized production; And employing the present invention can produce the Herba Artemisiae Annuae that contains finite concentration active ingredient and make liquid.Saved herb resource.

In < < Herba Artemisiae Annuae and artemisinin-based drug > > book, point out: " chemical composition of Herba Artemisiae Annuae, therefrom separation obtains more than 170 compounds so far ".The arteannuin of organic solvent extraction is 1 compound in these more than 170.

Toxicological test

From Gu doctor's nationality and modern pharmacology, toxicologic study, prove, Herba Artemisiae Annuae is nontoxic.This brew is to adopt ancient traditional zymotic technology, with Oryza glutinosa and song, takes unstrained spirits, there is no fear of generating noxious substance.But in order in person first to experience, first to have more than 10 people by the development personnel etc. that participate in Herba Artemisiae Annuae brew and take, the situation of non-toxic reaction occurs, wherein one, take continuously more than six months, 100 milliliters of every days during beginning, minute take for 3-4 time; 60 milliliters of every days after one month, minute 3 clothes, so far non-toxic reactions.

Do local skin and stick or be coated with and put on the skin, nonirritant and untoward reaction.

Clinical trial

Regular clinical trial, awaits doing clinical at the military region, Guangzhou Zong Yuan.The personnel that we have taken have following cognition:

Example 1, dermatosis.Dermatitis is more than half a year before two shanks, and pachylosis, itches, and especially itches evening to such an extent that sit up and grab.With the sediment that Herba Artemisiae Annuae is made liquid, be coated with and put, stick part on the skin, days 3 times, itch good day by day, after two weeks, itch and obviously alleviate, after one month, skin normally, is not itched and completely good.

Have and play pimple with it, bathtub water temperature when spending less than 35, is grabbed a Herba Artemisiae Annuae and is made the sediment of liquid and put into, people's bubble inside tens minutes, feel skin send out sliding, whole body is very comfortable.

Example 2, flu.Because of change in weather, lower the temperature, suffer from cold, cause sneeze, flow clear nasal mucus, throat pain.Buccal Herba Artemisiae Annuae make liquid, and be stained with plug in nasal cavity with cotton swab, the 3rd day all right, the symptom such as do not occur having a fever, coughing, spit.

Common cold of gastrointestinal type.Oral Herba Artemisiae Annuae wine liquid adds uses moxa stick moxibustion abdominal acupoints, takes effect immediately.

There is the large chronic ulcer of a Semen Coicis grain in example 3, oral cavity, be stained with Herba Artemisiae Annuae wine liquid smear with cotton swab, and after three days, ulcer surface disappears.

Example 4, effective to comedo.

Example 5, senile scratching where it itches, coat Herba Artemisiae Annuae wine liquid can be effectively at once.

Example 6, man, 81 years old.Blood pressure is higher, and at ordinary times more than 160/90, especially, in the situation that change in weather, air pressure are low, blood pressure shrinks and is pressed onto 170-190, and diastole is pressed onto 101, pulse occur obviously leaking jump, dizziness.Clinic gives clothes " An Bowei " invalid, and blood pressure lowering dose strengthens and can make blood pressure drops but surely not live.May 11 start to take Herba Artemisiae Annuae and make liquid, day 2 each 20-30 milliliters, blood pressure is gradually stabilized in normal range, and after one month, though Changes in weather is large, generally blood pressure is all at 121-138/80-86, and heart rate also becomes normally, without obviously leaking, jumps phenomenon.

Example 7, hyperthyroidism.Female, 75 years old.Within 2005, because of overworked, JIUYUE starts body weight and drops to 84 jin from 102 jin to November, within 2008, is down to less than 70 jin.Feel whole body heating, be usually sweating all over, heart beating obtains severe, and heart rate is everlasting more than 100 beats/min, and has obvious leakage to jump.Touching cervical region has lump, and doubtful thyroid goes wrong, and goes to hospital to do color ultrasound examination: lobus dexter thyroid size 2.2 * 2.8 * 3.5; Lobus sinister: 2.0 * 2.5 * 4.0.Profile is clear, the thick close skewness of luminous point in body of gland, and color Doppler is shown in more rich blood flow signal in showing.Isthmus thickness 0.7.Color ultrasound prompting: bilateral thyroid anthorisma (prompting: hyperthyroidism).Have a blood test: free triiodothyronine 12.15, free thyroxine 18.78, thyrotropin < 0.005, is diagnosed as hyperthyroidism.I am reluctant to accept isotope therapy.Start buccal Herba Artemisiae Annuae March 12 this year and make liquid, stick thyroid part simultaneously with sediment, clinical symptoms fades away, left side thyroid touched less than, right side also obviously dwindles (can touch and be less than treatment of distal thumb), to May 20, body weight increased by 10 jin, a traditional unit of weight.Arteannuin can be controlled systemic lupus erythematosus (sle), and hyperthyroidism is also a kind of autoimmune systemic disease, also occurs itch all over, and especially two shanks, the back of the body and upper arm, scrape and control rear discharge goo, and shank ankle is swollen to disappear; Eyes flow out colourless mucus, and throat has dope to spue, and the persistent period is longer.

Through the military region, Guangzhou, pharmacology portion of total institute makes cytotoxicity test to breast carcinoma, lung carcinoma cell, can in 20 hours, all kill.Existing WKG working animal experiment, after do again clinical.

Good effect of the present invention is:

1, the present invention is the pure wine liquid of Herba Artemisiae Annuae that brew goes out the whole active ingredient of Herba Artemisiae Annuae, and it has the drug action of Herba Artemisiae Annuae herb.Herba Artemisiae Annuae, derives from classics recipe in ancient times, refers to the drug effect of Herba Artemisiae Annuae herb.At present clinically application only have arteannuin and several derivant thereof, arteannuin is a compound in more than 170 of Herba Artemisiae Annuae.

2, technique of the present invention is the many strains " song " that adopt saccharifying, ferment parallel, first in vat, takes unstrained spirits, with " feeding method ", carries out compound fermentation; After pour into again little Cheng Zhong, low temperature (be preferably in below 15 ℃, under hypoxia (liquid level is apart from approximately 10 centimeters of the Cheng Kou that tie), lucifuge long-time (more than three months) carry out slowly fermenting, yeast phase is long, with abundant brew, go out active ingredient in water solublity and the undissolvable raw material of water, extraction ratio is higher than organic solvent.

4, wine liquid of the present invention is nontoxic, does not adopt edible ethanol or other Chinese liquor; , containing hormone, do not add any chemical industry flavoring agent and the additives such as pigment, essence, antiseptic.The arteannuin of organic solvent extraction is poisonous.

5, cost of the present invention is low, be expected to become valency low, nontoxic, can be the oral medicine that outpatient accepts.

6, wine liquid of the present invention is containing the beverage of a small amount of ethanol (wine degree 13 left and right, pol < 5, acidity < 0.45), absorb fast, very fast sensible collaterals of human blood vessels after taking.Oral type arteannuin easily decomposes in gastrointestinal, and excretion is fast, absorbs poor.

7, long shelf-life.Country points out about the pre-packaged beverage wine of < < label general rule > >: " wine and alcoholic strength surpass other alcoholic drink of 10 degree can exempt the mark shelf-life.”

" Herba Artemisiae Annuae is a strain JIUMINGCAO ", Herba Artemisiae Annuae comes from motherland's traditional medicine treasure-house, is willing to that the present invention can make Herba Artemisiae Annuae bring into play better its drug action, to bring benefit to the mankind.

List of references:

< < Herba Artemisiae Annuae and artemisinin-based drug > > slaughter cry of a deer write Chemical Industry Press and publish 2009.1

< < Compendium of Materia Medica > > is bright.Li Shizhen (1518-1593 A.D.) work People's Health Publisher publishes 1999.7

The complete works of > > of < < China medicated wine Chen Yi chief editor Shanghai science tech publishing house publishes 1991.5

< < yellow wine production technology > > light industry skilled worker training materials < < yellow wine production technology > > writes group and compiles 1983.1

< < wine, fruit wine and assembled alcoholic drinks production technology > > Chen Tao-sound chief editor Chemical Industry Press 1991.1

< < distillers yeast production practical technique > > Chinese food publishing house publishes

Claims (2)

1. Herba Artemisiae Annuae is made a preparation method for liquid, and its technological process comprises:

(1), clean artemisia leaf is pulverized, by bent grinds, standby;

(2), Oryza glutinosa soak, cook after cold water drench but, make to be cooled to 28～30 ℃, enter vat mixed song, take unstrained spirits, add a cover, be incubated;

(3), after 24-30 hour, go out to make liquid, start cylinder cap, cylinder temperature is lowered, and can discharge carbon dioxide, oxygen gas-supplying, promotes fermentation;

(4), after 24 hours, make piece again and float, pour in proportion cold water into, stripping and slicing, upset;

(5), again after one day, feeding, pours 30 jin of Herba Artemisiae Annuaes into for the first time, adds song and mixes thoroughly, carries out prior fermentation;

(6), drive rake after spending 12-18 hour, mix cylinder;

(7), next day, carry out feeding for the second time, in cylinder, pour 20 jin of Herba Artemisiae Annuaes into, add song, mix cylinder;

(8), again after one day, pour into the little field of razor clam, 30 jin of every field of razor clams, liquid level, apart from 10 centimeters, field of razor clam mouth, ties Cheng Kou, is beneficial to carry out slowly fermenting under low temperature, hypoxia; Slowly fermenting phase length is relevant with weather, more than 90 days;

(9), squeeze and filter;

(10), pour into the little field of razor clam, with Folium Nelumbinis, Folium Bambusae, mudding mouth, warehouse-in, displays more than one month;

(11), filter, bottle;

(12), paste label, packing;

(13), be up to the standards, dispatch from the factory;

Wherein, the ratio of raw material Oryza glutinosa, Herba Artemisiae Annuae, song and water is: 100 jin, Oryza glutinosa, 50 jin of Herba Artemisiae Annuae cured leafs, bent 1 jin, 150 jin, water.

2. the Herba Artemisiae Annuae that preparation method claimed in claim 1 obtains is made liquid.